Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002984

Physical Location & Seq

Genomic Coordinates Forward (+)

3148523 .. 3153422

Gene Structure 4900 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1028200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1737 bp

ATGATGGATATTACAGCAATAAAGTTGCTTGTTGAAGTTATTTTTCTCGCTTGTTGTGTTTTTGCTATAAACAACATTACCGGAGTCGAAGCGTTGCACAATGTTTATCCCGAGTTTCAGTCTCTTGCCGCCGTTAAGGTGAAACAAATCCACAGAACTGCGTTTCACTTTCAACCTCCCAGAAACTGGATTAACGGACCCATGTATTTTGGTGGTCTGTACCACTTGTTCTATCAGTACAATCCTGAAGGGGCTGTGTGGGGCAACATTGTTTGGGCCCATTCAGTGTCCAAGGACCTCATCAATTGGGAAGCTCTTGAGCCAGCCATATACCCATCCAAGCCCTTTGACGTAAATGGGTGTTGGTCCGGATCCGCCACTGTTCTCCCGGGTAACAAGCCCATTATGCTATACACCGGCGTCGACTCCCAAAACCGGCAAGTCCAAAACTATGCTATCCCGAAAAATGCGTCGGACCCCTATCTCCGTAAATGGATTAAGCCCGATAACAACCCGCTTGTGATTCCGGACACAGGTATCAACGCCACTGCGTTCCGAGACCCGACAACGGCTTGGTGGGGAAATGGACATTGGAAGATGTTGGTGGGAGGCAAAAGGAAGCACAGAGGAATGGCCCATTTGTATAGGAGCAAGGATTTTGTGCATTGGGTGAAGGCCCATCATCCTCTCCACTCTGCTCCCCAAACGGGTATGTGGGAGTGCCCCGATTTTTACCCGGTTCCATTGGTTGGAAAATTAGGGTTGGACACATCAAAAGTTGGTGGAGATGTGAAACATGTGTTGAAAGTGAGCCTTGATGAGACTAGGTATGAGTACTACACAGTTGGAAAATATTTTCCTGAGAAGGATACGTATGTTCCTGATAAAGAATTGGTGGATGGCTGGAGTGGATTGAGATATGACTATGGTAACTTTTATGCATCCAAAACTTTCTTTGATCCTGCAAAGAAGAGGAGAATTCTGTGGGGTTGGGCTAATGAATCTGATACAGCTAGCAATGATGTTGCTAAGGGATGGGCCGGAATTCAGACAATTCCAAGGGATGTGTGGCTTAGTCCTGATGGTAAACAATTGCTACAATGGCCTGTTGTAGAATTAGAAACTTTACGAGGGAAAAAGGTGGGCATCAACAATCAAATTCTCCAACAGGGATCTCATGTTGAAGTCAAAGGACTAACTGCTGCTCAGGCTGATGTTGAAGTTATTTTCTCACTTCCGAGTTTGGCCAAGGCTGAAGAATTTAACCCTAGCTGGAGTAACCTTGATGCCCAAACACTATGTGGTAAAAAGGGTTCAAAAATCCAAGGTGGAATTGGTCCTTTTGGACTCTTGACATTAGCTTCAGAAAACCTAGAGGAATTCACTCCTGTCTTCTTTAGGGTTTTCAAAGCTAAAGGCAGACACGTAGTTCTCATGTGCTCTGAAGCAACAAGTTCATCTTTGCAGGACAACCTGTACAGACCATCATTTGCTGGCTTTGTAGATGTAGATTTATCTCACTCTAAGAAGCTTTCCCTCAGGAGTTTGATTGATCATTCTGTTGTTGAAAGTTACGGAGCTGGAGGGAAAACATGCATCACATCTAGGGTTTACCCGACTCTAGCGGTAAACAACGGTGCTCACTTGTATGTGTTCAACAATGGGACTCAGCCTGTTACTGCGGAGAGTCTCAATGCATGGAGTATGAATGCTCCTACGATGAACAAGCAGATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0030312 GO:0033036 GO:0042742 GO:0043207 GO:0044464 GO:0050832 GO:0050896 GO:0051179 GO:0051704 GO:0051707 GO:0052575 GO:0052576 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

579

Amino Acids

64.6

Weight (kDa)

8.68

Isoelectric Point (pI)

30.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_32N	PF00251	56 - 369	5e-101	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	372 - 568	7e-22	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36190 AT3G52600 AT3G52600 AT3G52600
fragaria_vesca	FvH4_6g19270 FvH4_6g19270 FvH4_6g19271
malus_domestica	MD12G1028200.v1.1 MD14G1028500.v1.1
prunus_persica	Prupe.7G103100_v2.0.a1 Prupe.7G103200_v2.0.a1 Prupe.7G103300_v2.0.a1 Prupe.7G103400_v2.0.a1 Prupe.7G103400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0474371 RchiOBHm_Chr3g0474381
rosa_laevigata	RLG00000023930
rosa_multiflora	Rmu_co8392863.1_g000001 Rmu_co8415991.1_g000001 Rmu_co8444851.1_g000001 Rmu_sc0004507.1_g000003 Rmu_sc0004507.1_g000004
rosa_roxburghii	Rroxscaffold_6G00407310 Rroxscaffold_6G00407320
rosa_rugosa	Rorug03G0140200 Rorug03G0140300 Rorug03G0140400 Rorug03G0140500
rosa_samantha	Rh3AG191100 Rh3AG191200 Rh3BG220300 Rh3BG220400 Rh3CG215600 Rh3CG215700 Rh3DG216000 Rh3DG216100
rosa_wichuraiana	Rw3G017430 Rw3G017440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	186, 749
AccI	GTMKAC	1 cut(s)	423
AccIII	TCCGGA	2 cut(s)	368, 526
AciI	CCGC	5 cut(s)	129, 375, 515, 1625, 1682
AclWI	GGATC	4 cut(s)	366, 379, 953, 1180
AcoI	YGGCCR	1 cut(s)	1245
AcsI	RAATTY	5 cut(s)	978, 1044, 1158, 1259, 1379
AcuI	CTGAAG	4 cut(s)	267, 1275, 1347, 1464
AcyI	GRCGYC	1 cut(s)	420
AdeI	CACNNNGTG	1 cut(s)	1301
AfaI	GTAC	4 cut(s)	221, 239, 836, 1478
AfiI	CCNNNNNNNGG	6 cut(s)	186, 435, 533, 568, 707, 749
AflIII	ACRYGT	2 cut(s)	796, 1423
AgsI	TTSAA	9 cut(s)	35, 173, 805, 1184, 1220, 1317, 1408, 1568, 1657
AjuI	GAANNNNNNNTTGG	2 cut(s)	574, 606
AluBI	AGCT	7 cut(s)	314, 1013, 1272, 1361, 1412, 1531, 1580
AluI	AGCT	7 cut(s)	314, 1013, 1272, 1361, 1412, 1531, 1580
Alw21I	GWGCWC	2 cut(s)	1442, 1642
Alw26I	GTCTC	4 cut(s)	126, 552, 815, 1694
AlwI	GGATC	4 cut(s)	366, 379, 953, 1180
AlwNI	CAGNNNCTG	1 cut(s)	186
Ama87I	CYCGRG	2 cut(s)	110, 388
Aor13HI	TCCGGA	2 cut(s)	368, 526
AoxI	GGCC	6 cut(s)	276, 633, 675, 1038, 1103, 1245
ApaI	GGGCCC	1 cut(s)	280
ApeKI	GCWGC	1 cut(s)	1202
ApoI	RAATTY	5 cut(s)	978, 1044, 1158, 1259, 1379
ArsI	GACNNNNNNTTYG	2 cut(s)	455, 487
AsuC2I	CCSGG	3 cut(s)	389, 390, 737
AsuHPI	GGTGA	2 cut(s)	151, 682
AsuNHI	GCTAGC	1 cut(s)	1013
AvaI	CYCGRG	2 cut(s)	110, 388
AvaII	GGWCC	5 cut(s)	197, 295, 366, 475, 1337
AxyI	CCTNAGG	1 cut(s)	1538
BaeGI	GKGCMC	2 cut(s)	280, 725
BaeI	ACNNNNGTAYC	4 cut(s)	520, 553, 861, 894
BalI	TGGCCA	1 cut(s)	1247
BamHI	GGATCC	1 cut(s)	371
BanII	GRGCYC	1 cut(s)	280
BbsI	GAAGAC	1 cut(s)	1384
Bbv12I	GWGCWC	2 cut(s)	1442, 1642
BbvI	GCAGC	1 cut(s)	1189
BccI	CCATC	6 cut(s)	343, 687, 893, 1029, 1076, 1492
BceAI	ACGGC	2 cut(s)	116, 585
BciVI	GTATCC	1 cut(s)	862
BclI	TGATCA	1 cut(s)	1552
BcnI	CCSGG	3 cut(s)	389, 390, 737
BcoDI	GTCTC	4 cut(s)	126, 552, 815, 1694
BfaI	CTAG	6 cut(s)	825, 1014, 1269, 1373, 1605, 1622
BfuI	GTATCC	1 cut(s)	862
BisI	GCNGC	2 cut(s)	129, 1203
BlsI	GCNGC	2 cut(s)	130, 1204
BmcAI	AGTACT	1 cut(s)	836
Bme1390I	CCNGG	3 cut(s)	389, 390, 737
Bme18I	GGWCC	5 cut(s)	197, 295, 366, 475, 1337
BmeT110I	CYCGRG	2 cut(s)	110, 388
BmiI	GGNNCC	5 cut(s)	199, 278, 373, 477, 741
BmrFI	CCNGG	3 cut(s)	389, 390, 737
BmsI	GCATC	4 cut(s)	950, 1155, 1276, 1605
BmtI	GCTAGC	1 cut(s)	1017
BpiI	GAAGAC	1 cut(s)	1384
BpmI	CTGGAG	3 cut(s)	925, 1294, 1602
Bpu10I	CCTNAGC	2 cut(s)	1029, 1206
BpuEI	CTTGAG	1 cut(s)	338
BpuMI	CCSGG	3 cut(s)	389, 390, 737
BsaAI	YACGTR	2 cut(s)	873, 1426
BsaHI	GRCGYC	1 cut(s)	420
BsaI	GGTCTC	1 cut(s)	552
BsaJI	CCNNGG	5 cut(s)	291, 388, 1058, 1248, 1324
BsaWI	WCCGGW	3 cut(s)	80, 368, 526
Bsc4I	CCNNNNNNNGG	6 cut(s)	186, 435, 533, 568, 707, 749
Bse118I	RCCGGY	2 cut(s)	416, 435
Bse1I	ACTGG	1 cut(s)	191
Bse21I	CCTNAGG	1 cut(s)	1538
Bse3DI	GCAATG	1 cut(s)	1024
BseAI	TCCGGA	2 cut(s)	368, 526
BseDI	CCNNGG	5 cut(s)	291, 388, 1058, 1248, 1324
BseGI	GGATG	6 cut(s)	335, 682, 904, 941, 1040, 1069
BseLI	CCNNNNNNNGG	6 cut(s)	186, 435, 533, 568, 707, 749
BseMI	GCAATG	1 cut(s)	1024
BseMII	CTCAG	4 cut(s)	852, 1220, 1552, 1682
BseNI	ACTGG	1 cut(s)	191
BseRI	GAGGAG	1 cut(s)	988
BseSI	GKGCMC	2 cut(s)	280, 725
BseXI	GCAGC	1 cut(s)	1189
BshFI	GGCC	6 cut(s)	278, 635, 677, 1040, 1105, 1247
BsiHKAI	GWGCWC	2 cut(s)	1442, 1642
BsiHKCI	CYCGRG	2 cut(s)	110, 388
BsiSI	CCGG	8 cut(s)	81, 369, 389, 417, 436, 527, 737, 1041
BslFI	GGGAC	1 cut(s)	1678
BslI	CCNNNNNNNGG	6 cut(s)	186, 435, 533, 568, 707, 749
BsmAI	GTCTC	4 cut(s)	126, 552, 815, 1694
BsmFI	GGGAC	1 cut(s)	1678
BsmI	GAATGC	1 cut(s)	1714
BsnI	GGCC	6 cut(s)	278, 635, 677, 1040, 1105, 1247
Bso31I	GGTCTC	1 cut(s)	552
BsoBI	CYCGRG	2 cut(s)	110, 388
Bsp120I	GGGCCC	1 cut(s)	276
Bsp1286I	GDGCHC	4 cut(s)	280, 725, 1442, 1642
Bsp13I	TCCGGA	2 cut(s)	368, 526
Bsp1407I	TGTACA	1 cut(s)	1476
Bsp143I	GATC	4 cut(s)	371, 958, 1172, 1552
BspACI	CCGC	5 cut(s)	129, 375, 515, 1625, 1682
BspANI	GGCC	6 cut(s)	278, 635, 677, 1040, 1105, 1247
BspCNI	CTCAG	4 cut(s)	853, 1219, 1551, 1681
BspEI	TCCGGA	2 cut(s)	368, 526
BspLI	GGNNCC	5 cut(s)	199, 278, 373, 477, 741
BspOI	GCTAGC	1 cut(s)	1017
BspPI	GGATC	4 cut(s)	366, 379, 953, 1180
BspTNI	GGTCTC	1 cut(s)	552
BsrDI	GCAATG	1 cut(s)	1024
BsrFI	RCCGGY	2 cut(s)	416, 435
BsrGI	TGTACA	1 cut(s)	1476
BsrI	ACTGG	1 cut(s)	191
BssAI	RCCGGY	2 cut(s)	416, 435
BssECI	CCNNGG	5 cut(s)	291, 388, 1058, 1248, 1324
BssMI	GATC	4 cut(s)	371, 958, 1172, 1552
BssNI	GRCGYC	1 cut(s)	420
BssT1I	CCWWGG	4 cut(s)	291, 1058, 1248, 1324
Bst4CI	ACNGT	3 cut(s)	382, 844, 1637
Bst6I	CTCTTC	1 cut(s)	965
BstACI	GRCGYC	1 cut(s)	420
BstAUI	TGTACA	1 cut(s)	1476
BstBAI	YACGTR	2 cut(s)	873, 1426
BstC8I	GCNNGC	3 cut(s)	324, 1015, 1495
BstDEI	CTNAG	7 cut(s)	861, 1029, 1073, 1206, 1524, 1538, 1668
BstF5I	GGATG	6 cut(s)	335, 682, 904, 941, 1040, 1069
BstKTI	GATC	4 cut(s)	374, 961, 1175, 1555
BstMAI	GTCTC	4 cut(s)	126, 552, 815, 1694
BstMBI	GATC	4 cut(s)	371, 958, 1172, 1552
BstMWI	GCNNNNNNNGC	3 cut(s)	406, 1102, 1208
BstNSI	RCATGY	2 cut(s)	800, 1596
BstSCI	CCNGG	3 cut(s)	387, 388, 735
BstSLI	GKGCMC	2 cut(s)	280, 725
BstSNI	TACGTA	1 cut(s)	873
BstV1I	GCAGC	1 cut(s)	1189
BstV2I	GAAGAC	1 cut(s)	1384
BstX2I	RGATCY	2 cut(s)	371, 1172
BstYI	RGATCY	2 cut(s)	371, 1172
Bsu36I	CCTNAGG	1 cut(s)	1538
BsuI	GTATCC	1 cut(s)	862
BsuRI	GGCC	6 cut(s)	278, 635, 677, 1040, 1105, 1247
BtsCI	GGATG	6 cut(s)	335, 682, 904, 941, 1040, 1069
BtsI	GCAGTG	1 cut(s)	546
BtsIMutI	CAGTG	3 cut(s)	291, 378, 546
Cac8I	GCNNGC	3 cut(s)	324, 1015, 1495
CaiI	CAGNNNCTG	1 cut(s)	186
Cfr10I	RCCGGY	2 cut(s)	416, 435
Cfr9I	CCCGGG	1 cut(s)	388
CseI	GACGC	2 cut(s)	409, 459
Csp6I	GTAC	4 cut(s)	220, 238, 835, 1477
CviAII	CATG	6 cut(s)	202, 797, 1178, 1435, 1593, 1698
CviQI	GTAC	4 cut(s)	220, 238, 835, 1477
DdeI	CTNAG	7 cut(s)	861, 1029, 1073, 1206, 1524, 1538, 1668
DpnI	GATC	4 cut(s)	373, 960, 1174, 1554
DpnII	GATC	4 cut(s)	371, 958, 1172, 1552
DraIII	CACNNNGTG	1 cut(s)	1301
EaeI	YGGCCR	1 cut(s)	1245
Eam1104I	CTCTTC	1 cut(s)	965
EarI	CTCTTC	1 cut(s)	965
EciI	GGCGGA	1 cut(s)	364
Eco105I	TACGTA	1 cut(s)	873
Eco130I	CCWWGG	4 cut(s)	291, 1058, 1248, 1324
Eco24I	GRGCYC	1 cut(s)	280
Eco31I	GGTCTC	1 cut(s)	552
Eco47I	GGWCC	5 cut(s)	197, 295, 366, 475, 1337
Eco57I	CTGAAG	4 cut(s)	267, 1275, 1347, 1464
Eco81I	CCTNAGG	1 cut(s)	1538
Eco88I	CYCGRG	2 cut(s)	110, 388
EcoO109I	RGGNCCY	1 cut(s)	295
EcoRI	GAATTC	3 cut(s)	978, 1044, 1379
EcoT14I	CCWWGG	4 cut(s)	291, 1058, 1248, 1324
EcoT22I	ATGCAT	3 cut(s)	943, 1598, 1699
EcoT38I	GRGCYC	1 cut(s)	280
ErhI	CCWWGG	4 cut(s)	291, 1058, 1248, 1324
FaeI	CATG	6 cut(s)	205, 800, 1181, 1438, 1596, 1701
FalI	AAGNNNNNCTT	4 cut(s)	798, 830, 1444, 1476
FaqI	GGGAC	1 cut(s)	1678
FatI	CATG	6 cut(s)	201, 796, 1177, 1434, 1592, 1697
FauI	CCCGC	1 cut(s)	522
FbaI	TGATCA	1 cut(s)	1552
FblI	GTMKAC	1 cut(s)	423
Fnu4HI	GCNGC	2 cut(s)	129, 1203
FokI	GGATG	6 cut(s)	322, 669, 911, 928, 1047, 1076
FriOI	GRGCYC	1 cut(s)	280
Fsp4HI	GCNGC	2 cut(s)	129, 1203
FspBI	CTAG	6 cut(s)	825, 1014, 1269, 1373, 1605, 1622
GluI	GCNGC	2 cut(s)	129, 1203
GsuI	CTGGAG	3 cut(s)	925, 1294, 1602
HaeIII	GGCC	6 cut(s)	278, 635, 677, 1040, 1105, 1247
HapII	CCGG	8 cut(s)	81, 369, 389, 417, 436, 527, 737, 1041
HgaI	GACGC	2 cut(s)	409, 459
Hin1I	GRCGYC	1 cut(s)	420
Hin1II	CATG	6 cut(s)	205, 800, 1181, 1438, 1596, 1701
HincII	GTYRAC	1 cut(s)	424
HindII	GTYRAC	1 cut(s)	424
HindIII	AAGCTT	1 cut(s)	1529
HinfI	GANTC	8 cut(s)	84, 425, 523, 1001, 1347, 1618, 1666, 1687
HpaII	CCGG	8 cut(s)	81, 369, 389, 417, 436, 527, 737, 1041
HphI	GGTGA	2 cut(s)	151, 682
Hpy166II	GTNNAC	4 cut(s)	424, 1088, 1612, 1630
Hpy188I	TCNGA	7 cut(s)	475, 557, 1006, 1050, 1239, 1366, 1444
Hpy8I	GTNNAC	4 cut(s)	424, 1088, 1612, 1630
Hpy99I	CGWCG	2 cut(s)	425, 475
HpyAV	CCTTC	3 cut(s)	242, 667, 859
HpyCH4III	ACNGT	3 cut(s)	382, 844, 1637
HpyCH4IV	ACGT	3 cut(s)	351, 872, 1425
HpyCH4V	TGCA	7 cut(s)	97, 664, 941, 965, 1465, 1596, 1697
HpyF10VI	GCNNNNNNNGC	3 cut(s)	406, 1102, 1208
HpyF3I	CTNAG	7 cut(s)	861, 1029, 1073, 1206, 1524, 1538, 1668
HpySE526I	ACGT	3 cut(s)	351, 872, 1425
Hsp92I	GRCGYC	1 cut(s)	420
Hsp92II	CATG	6 cut(s)	205, 800, 1181, 1438, 1596, 1701
Kpn2I	TCCGGA	2 cut(s)	368, 526
Ksp22I	TGATCA	1 cut(s)	1552
Kzo9I	GATC	4 cut(s)	371, 958, 1172, 1552
LmnI	GCTCC	4 cut(s)	648, 703, 1577, 1717
Lsp1109I	GCAGC	1 cut(s)	1189
LweI	GCATC	4 cut(s)	950, 1155, 1276, 1605
MaeI	CTAG	6 cut(s)	825, 1014, 1269, 1373, 1605, 1622
MaeII	ACGT	3 cut(s)	351, 872, 1425
MaeIII	GTNAC	5 cut(s)	392, 929, 1277, 1571, 1675
MalI	GATC	4 cut(s)	373, 960, 1174, 1554
MboI	GATC	4 cut(s)	371, 958, 1172, 1552
MboII	GAAGA	4 cut(s)	607, 982, 1268, 1384
MfeI	CAATTG	2 cut(s)	304, 1091
MflI	RGATCY	2 cut(s)	371, 1172
MhlI	GDGCHC	4 cut(s)	280, 725, 1442, 1642
MlsI	TGGCCA	1 cut(s)	1247
MluNI	TGGCCA	1 cut(s)	1247
MlyI	GAGTC	6 cut(s)	93, 419, 1341, 1612, 1660, 1696
MmeI	TCCRAC	5 cut(s)	453, 730, 744, 826, 1189
Mox20I	TGGCCA	1 cut(s)	1247
Mph1103I	ATGCAT	3 cut(s)	943, 1598, 1699
MroI	TCCGGA	2 cut(s)	368, 526
MscI	TGGCCA	1 cut(s)	1247
MseI	TTAA	4 cut(s)	135, 192, 498, 1263
MslI	CAYNNNNRTG	1 cut(s)	1647
Msp20I	TGGCCA	1 cut(s)	1247
MspI	CCGG	8 cut(s)	81, 369, 389, 417, 436, 527, 737, 1041
MspR9I	CCNGG	3 cut(s)	389, 390, 737
MunI	CAATTG	2 cut(s)	304, 1091
Mva1269I	GAATGC	1 cut(s)	1714
MwoI	GCNNNNNNNGC	3 cut(s)	406, 1102, 1208
NciI	CCSGG	3 cut(s)	389, 390, 737
NdeII	GATC	4 cut(s)	371, 958, 1172, 1552
NheI	GCTAGC	1 cut(s)	1013
NlaIII	CATG	6 cut(s)	205, 800, 1181, 1438, 1596, 1701
NlaIV	GGNNCC	5 cut(s)	199, 278, 373, 477, 741
NsiI	ATGCAT	3 cut(s)	943, 1598, 1699
NspI	RCATGY	2 cut(s)	800, 1596
PciI	ACATGT	1 cut(s)	796
PctI	GAATGC	1 cut(s)	1714
PfeI	GAWTC	2 cut(s)	523, 1001
PflMI	CCANNNNNTGG	2 cut(s)	186, 749
PkrI	GCNGC	2 cut(s)	130, 1204
PleI	GAGTC	6 cut(s)	92, 419, 1341, 1612, 1660, 1695
PpsI	GAGTC	6 cut(s)	92, 419, 1341, 1612, 1660, 1695
Ppu21I	YACGTR	2 cut(s)	873, 1426
PpuMI	RGGWCCY	1 cut(s)	295
PscI	ACATGT	1 cut(s)	796
Psp5II	RGGWCCY	1 cut(s)	295
PspN4I	GGNNCC	5 cut(s)	199, 278, 373, 477, 741
PspOMI	GGGCCC	1 cut(s)	276
PspPPI	RGGWCCY	1 cut(s)	295
PsrI	GAACNNNNNNTAC	4 cut(s)	212, 244, 1297, 1329
PstNI	CAGNNNCTG	1 cut(s)	186
PsuI	RGATCY	2 cut(s)	371, 1172
RsaI	GTAC	4 cut(s)	221, 239, 836, 1478
RsaNI	GTAC	4 cut(s)	220, 238, 835, 1477
RseI	CAYNNNNRTG	1 cut(s)	1647
SalI	GTCGAC	1 cut(s)	422
SaqAI	TTAA	4 cut(s)	135, 192, 498, 1263
SatI	GCNGC	2 cut(s)	129, 1203
Sau3AI	GATC	4 cut(s)	371, 958, 1172, 1552
ScaI	AGTACT	1 cut(s)	836
SchI	GAGTC	6 cut(s)	93, 419, 1341, 1612, 1660, 1696
ScrFI	CCNGG	3 cut(s)	389, 390, 737
SduI	GDGCHC	4 cut(s)	280, 725, 1442, 1642
SfaNI	GCATC	4 cut(s)	950, 1155, 1276, 1605
SgrAI	CRCCGGYG	1 cut(s)	416
SinI	GGWCC	5 cut(s)	197, 295, 366, 475, 1337
SmaI	CCCGGG	1 cut(s)	390
SmiMI	CAYNNNNRTG	1 cut(s)	1647
SmlI	CTYRAG	1 cut(s)	317
SmoI	CTYRAG	1 cut(s)	317
SnaBI	TACGTA	1 cut(s)	873
SsiI	CCGC	5 cut(s)	129, 375, 515, 1625, 1682
SspI	AATATT	1 cut(s)	854
SspMI	CTAG	6 cut(s)	825, 1014, 1269, 1373, 1605, 1622
StyD4I	CCNGG	3 cut(s)	387, 388, 735
StyI	CCWWGG	4 cut(s)	291, 1058, 1248, 1324
TaaI	ACNGT	3 cut(s)	382, 844, 1637
TaiI	ACGT	3 cut(s)	354, 875, 1428
TaqI	TCGA	2 cut(s)	87, 423
TatI	WGTACW	3 cut(s)	237, 834, 1476
TauI	GCSGC	1 cut(s)	131
TfiI	GAWTC	2 cut(s)	523, 1001
Tru1I	TTAA	4 cut(s)	135, 192, 498, 1263
Tru9I	TTAA	4 cut(s)	135, 192, 498, 1263
TscAI	CASTG	3 cut(s)	291, 385, 553
TseI	GCWGC	1 cut(s)	1202
TspDTI	ATGAA	4 cut(s)	1014, 1446, 1721, 1736
TspGWI	ACGGA	3 cut(s)	210, 476, 1590
TspMI	CCCGGG	1 cut(s)	388
TspRI	CASTG	3 cut(s)	291, 385, 553
Van91I	CCANNNNNTGG	2 cut(s)	186, 749
VpaK11BI	GGWCC	5 cut(s)	197, 295, 366, 475, 1337
XapI	RAATTY	5 cut(s)	978, 1044, 1158, 1259, 1379
XceI	RCATGY	2 cut(s)	800, 1596
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1065, 1298, 1331
XmaI	CCCGGG	1 cut(s)	388
XmiI	GTMKAC	1 cut(s)	423
XspI	CTAG	6 cut(s)	825, 1014, 1269, 1373, 1605, 1622
ZrmI	AGTACT	1 cut(s)	836
Zsp2I	ATGCAT	3 cut(s)	943, 1598, 1699

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.