Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0002984

Physical Location & Seq

Genomic Coordinates Reverse (-)

30002661 .. 30005838

Gene Structure 3178 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00407320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGTCCTATTAGAGATGGCAAAAATCCCCCAGCGGGCGGAGCTCCATTGGATACCCGCCCCTAATGGGGGGAGAATTCGGGGACAAATGGGGAATGGGGATGGGGATCCCCAATCCCCGCTATCTAAGATTGGGGATGGGGCGGGGATGTACAATCCCAAAGGAGCTGTTTGGGGCAACATTGTATGGGCCCATTCAGTCTCTAAGGATCTGATCAATTGGCAAGCCCTTGAACCCGCCATATACCCATCCAAGCCCTTTGACATAAACGGGTGCTGGTCCGGATCCGCCACCCTTCTCCCGGGCAACAAGCCTATCATCCTCTACACCGGACTTGACCCGGAAAAGCGCCAGGTCCAAAACTACGCGGTGCCCGCAAATGTGTCCGACCCATATCTACGCAAATGGGTTAAGCCCGACAACAACCCGCTAGTGGTTCCGGATGCAAGTATGAATGCATCCCAGTTCCGTGACCCGACAACCGCTTGGTGGAGCAATGGGCACTGGAAGATGTTGGTTGGTGGCAAGAGAAAGCATAGAGGGATGGCTCATTTGTATAGGAGCAAGGATTTTGTTTATTGGGTCAAGGCCCAGCACCCGCTTCACTCTGCCCCGAATACGGGTATGTGGGAATGCCCGGATTTTTACCCGGTTTCATTGAAGGGCAAGGCAGGGTTGGACACATCAAAAGATGGTGGGAATGTGAAGCATGTTTTGAAAGTGAGCCTTGATGAGACTAGGTATGACTACTATACTGTTGGAAAATACTTTCCTAAACAAGATAGGTATGTTCCTGATAAGGAATTGGTGGATGGTTGGGCTGGATTGAGATATGATTATGGGAACTTCTATGCTTCCAAAACTTTCTTTGATCCTGCAAAGAATAGAAGAATTTTGTGGGGCTGGGCTAATGAGTCTGATACTCCTAACAATGATGTTGAAAAGGGATGGGCTGGAGTTCAGGCCGATGTTGAAGTTATATTCTCTTTCAAGAGCTTGGACAAAGCAGAGAAGTTTGACCCTAAATGGTCAAACCTTGATGCCCAAGCAATATGTGGACTAACGGGTTCAAAAATTGGAGGTGGCATTGGCCCATTTGGGCTATTGACTTTAGCTTCACAAAACCTAGAGGAATTCACTCCTGTCTTCTTTAGGGTGTTCAAAACTCAAGGCAATAAGCATAAGGTTCTCCTGTGCTCTGATGCAAGAAGTTCCTCTTGGCAGGATAATCTGTACAAACCATCATTTGCTGGTTTTGTAGATGTAGACTTATCTGATAAGAAGCTCTCCCTCAGGAGTTTGATTGATCATTCTGTTGTTGAGAGTTTTGGAGCTGGAGGAAAAACATGCATTACTTCTAGGGTTTACCCCACTCTAGCTGTTGACAATAAAGCTCACTTGTATGTGTTCAACAATGGGACTGAGGCTGTCACTATTGAGAGTCTCGATGCGTGGACTATGAATGCTCCTAAGTTGATGAACCAATATTCCCAGGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0030312 GO:0033036 GO:0042742 GO:0043207 GO:0044464 GO:0050832 GO:0050896 GO:0051179 GO:0051704 GO:0051707 GO:0052575 GO:0052576 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

499

Amino Acids

55.64

Weight (kDa)

8.76

Isoelectric Point (pI)

29.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_32N	PF00251	51 - 322	1.9e-79	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	317 - 485	8.7e-21	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36190 AT3G52600 AT3G52600 AT3G52600
fragaria_vesca	FvH4_6g19270 FvH4_6g19270 FvH4_6g19271
malus_domestica	MD12G1028200.v1.1 MD14G1028500.v1.1
prunus_persica	Prupe.7G103100_v2.0.a1 Prupe.7G103200_v2.0.a1 Prupe.7G103300_v2.0.a1 Prupe.7G103400_v2.0.a1 Prupe.7G103400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0474371 RchiOBHm_Chr3g0474381
rosa_laevigata	RLG00000023930
rosa_multiflora	Rmu_co8392863.1_g000001 Rmu_co8415991.1_g000001 Rmu_co8444851.1_g000001 Rmu_sc0004507.1_g000003 Rmu_sc0004507.1_g000004
rosa_roxburghii	Rroxscaffold_6G00407310 Rroxscaffold_6G00407320
rosa_rugosa	Rorug03G0140200 Rorug03G0140300 Rorug03G0140400 Rorug03G0140500
rosa_samantha	Rh3AG191100 Rh3AG191200 Rh3BG220300 Rh3BG220400 Rh3CG215600 Rh3CG215700 Rh3DG216000 Rh3DG216100
rosa_wichuraiana	Rw3G017430 Rw3G017440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	370
AccI	GTMKAC	1 cut(s)	1266
AccII	CGCG	1 cut(s)	368
AccIII	TCCGGA	2 cut(s)	281, 439
AclWI	GGATC	6 cut(s)	101, 114, 216, 279, 292, 866
AcsI	RAATTY	3 cut(s)	75, 891, 1133
AfaI	GTAC	2 cut(s)	152, 1235
AgsI	TTSAA	9 cut(s)	233, 661, 718, 941, 974, 991, 1071, 1162, 1411
AjnI	CCWGG	2 cut(s)	351, 1491
AjuI	GAANNNNNNNTTGG	2 cut(s)	1201, 1233
AloI	GAACNNNNNNTCC	1 cut(s)	1472
AluBI	AGCT	8 cut(s)	43, 167, 996, 1115, 1285, 1334, 1379, 1394
AluI	AGCT	8 cut(s)	43, 167, 996, 1115, 1285, 1334, 1379, 1394
Alw21I	GWGCWC	2 cut(s)	45, 1199
Alw26I	GTCTC	3 cut(s)	205, 728, 1448
AlwI	GGATC	6 cut(s)	101, 114, 216, 279, 292, 866
Ama87I	CYCGRG	1 cut(s)	301
Aor13HI	TCCGGA	2 cut(s)	281, 439
AoxI	GGCC	4 cut(s)	189, 588, 963, 1090
ApaI	GGGCCC	1 cut(s)	193
ApoI	RAATTY	3 cut(s)	75, 891, 1133
AspLEI	GCGC	1 cut(s)	351
AspS9I	GGNCC	7 cut(s)	4, 189, 190, 279, 355, 589, 1091
AsuC2I	CCSGG	5 cut(s)	302, 303, 341, 638, 650
AvaI	CYCGRG	1 cut(s)	301
AvaII	GGWCC	3 cut(s)	4, 279, 355
AxyI	CCTNAGG	1 cut(s)	1292
BaeGI	GKGCMC	3 cut(s)	193, 375, 504
BamHI	GGATCC	2 cut(s)	106, 284
BanI	GGYRCC	1 cut(s)	370
BanII	GRGCYC	2 cut(s)	45, 193
BbsI	GAAGAC	1 cut(s)	1138
Bbv12I	GWGCWC	2 cut(s)	45, 1199
BccI	CCATC	9 cut(s)	10, 95, 131, 256, 538, 686, 806, 942, 1249
BciT130I	CCWGG	2 cut(s)	353, 1493
BciVI	GTATCC	1 cut(s)	45
BclI	TGATCA	2 cut(s)	213, 1306
BcnI	CCSGG	5 cut(s)	302, 303, 341, 638, 650
BcoDI	GTCTC	3 cut(s)	205, 728, 1448
BfaI	CTAG	6 cut(s)	431, 738, 1127, 1359, 1376, 1498
BfoI	RGCGCY	1 cut(s)	352
BfuI	GTATCC	1 cut(s)	45
Bme1390I	CCNGG	7 cut(s)	302, 303, 341, 353, 638, 650, 1493
Bme18I	GGWCC	3 cut(s)	4, 279, 355
BmeT110I	CYCGRG	1 cut(s)	301
BmgT120I	GGNCC	7 cut(s)	4, 189, 190, 279, 355, 589, 1091
BmiI	GGNNCC	5 cut(s)	108, 191, 286, 372, 438
BmrFI	CCNGG	7 cut(s)	302, 303, 341, 353, 638, 650, 1493
BmrI	ACTGGG	1 cut(s)	457
BmsI	GCATC	5 cut(s)	433, 467, 1030, 1192, 1438
BmuI	ACTGGG	1 cut(s)	457
BpiI	GAAGAC	1 cut(s)	1138
BpmI	CTGGAG	2 cut(s)	975, 1356
BpuEI	CTTGAG	1 cut(s)	1152
BpuMI	CCSGG	5 cut(s)	302, 303, 341, 638, 650
BsaBI	GATNNNNATC	1 cut(s)	105
BsaJI	CCNNGG	2 cut(s)	301, 1491
BsaWI	WCCGGW	3 cut(s)	281, 329, 439
Bse1I	ACTGG	2 cut(s)	463, 509
Bse21I	CCTNAGG	1 cut(s)	1292
Bse3DI	GCAATG	1 cut(s)	502
Bse8I	GATNNNNATC	1 cut(s)	105
BseAI	TCCGGA	2 cut(s)	281, 439
BseBI	CCWGG	2 cut(s)	353, 1493
BseDI	CCNNGG	2 cut(s)	301, 1491
BseJI	GATNNNNATC	1 cut(s)	105
BseMI	GCAATG	1 cut(s)	502
BseMII	CTCAG	2 cut(s)	1306, 1413
BseNI	ACTGG	2 cut(s)	463, 509
BseSI	GKGCMC	3 cut(s)	193, 375, 504
BseYI	CCCAGC	3 cut(s)	30, 591, 903
Bsh1236I	CGCG	1 cut(s)	368
BshFI	GGCC	4 cut(s)	191, 590, 965, 1092
BshNI	GGYRCC	1 cut(s)	370
BsiHKAI	GWGCWC	2 cut(s)	45, 1199
BsiHKCI	CYCGRG	1 cut(s)	301
BsiSI	CCGG	7 cut(s)	282, 302, 330, 341, 440, 638, 650
BslFI	GGGAC	2 cut(s)	96, 1432
BsmAI	GTCTC	3 cut(s)	205, 728, 1448
BsmFI	GGGAC	2 cut(s)	96, 1432
BsmI	GAATGC	3 cut(s)	460, 638, 1468
BsnI	GGCC	4 cut(s)	191, 590, 965, 1092
BsoBI	CYCGRG	1 cut(s)	301
Bsp120I	GGGCCC	1 cut(s)	189
Bsp1286I	GDGCHC	5 cut(s)	45, 193, 375, 504, 1199
Bsp13I	TCCGGA	2 cut(s)	281, 439
Bsp1407I	TGTACA	2 cut(s)	150, 1233
Bsp143I	GATC	6 cut(s)	106, 208, 213, 284, 871, 1306
BspANI	GGCC	4 cut(s)	191, 590, 965, 1092
BspCNI	CTCAG	2 cut(s)	1305, 1414
BspEI	TCCGGA	2 cut(s)	281, 439
BspFNI	CGCG	1 cut(s)	368
BspLI	GGNNCC	5 cut(s)	108, 191, 286, 372, 438
BspPI	GGATC	6 cut(s)	101, 114, 216, 279, 292, 866
BspT107I	GGYRCC	1 cut(s)	370
BsrDI	GCAATG	1 cut(s)	502
BsrGI	TGTACA	2 cut(s)	150, 1233
BsrI	ACTGG	2 cut(s)	463, 509
BssECI	CCNNGG	2 cut(s)	301, 1491
BssMI	GATC	6 cut(s)	106, 208, 213, 284, 871, 1306
Bst2UI	CCWGG	2 cut(s)	353, 1493
Bst4CI	ACNGT	1 cut(s)	757
BstAUI	TGTACA	2 cut(s)	150, 1233
BstC8I	GCNNGC	3 cut(s)	36, 225, 375
BstDEI	CTNAG	5 cut(s)	126, 204, 1292, 1422, 1470
BstFNI	CGCG	1 cut(s)	368
BstH2I	RGCGCY	1 cut(s)	352
BstHHI	GCGC	1 cut(s)	351
BstKTI	GATC	6 cut(s)	109, 211, 216, 287, 874, 1309
BstMAI	GTCTC	3 cut(s)	205, 728, 1448
BstMBI	GATC	6 cut(s)	106, 208, 213, 284, 871, 1306
BstMWI	GCNNNNNNNGC	2 cut(s)	40, 374
BstNI	CCWGG	2 cut(s)	353, 1493
BstNSI	RCATGY	2 cut(s)	713, 1350
BstSCI	CCNGG	7 cut(s)	300, 301, 339, 351, 636, 648, 1491
BstSLI	GKGCMC	3 cut(s)	193, 375, 504
BstUI	CGCG	1 cut(s)	368
BstV2I	GAAGAC	1 cut(s)	1138
BstX2I	RGATCY	3 cut(s)	106, 208, 284
BstYI	RGATCY	3 cut(s)	106, 208, 284
Bsu36I	CCTNAGG	1 cut(s)	1292
BsuI	GTATCC	1 cut(s)	45
BsuRI	GGCC	4 cut(s)	191, 590, 965, 1092
BtsIMutI	CAGTG	1 cut(s)	502
Cac8I	GCNNGC	3 cut(s)	36, 225, 375
CfoI	GCGC	1 cut(s)	351
Cfr13I	GGNCC	7 cut(s)	4, 189, 190, 279, 355, 589, 1091
Cfr9I	CCCGGG	1 cut(s)	301
Csp6I	GTAC	2 cut(s)	151, 1234
CviAII	CATG	2 cut(s)	710, 1347
CviQI	GTAC	2 cut(s)	151, 1234
DdeI	CTNAG	5 cut(s)	126, 204, 1292, 1422, 1470
DpnI	GATC	6 cut(s)	108, 210, 215, 286, 873, 1308
DpnII	GATC	6 cut(s)	106, 208, 213, 284, 871, 1306
EciI	GGCGGA	2 cut(s)	53, 277
Ecl136II	GAGCTC	1 cut(s)	43
Eco24I	GRGCYC	2 cut(s)	45, 193
Eco47I	GGWCC	3 cut(s)	4, 279, 355
Eco53kI	GAGCTC	1 cut(s)	43
Eco81I	CCTNAGG	1 cut(s)	1292
Eco88I	CYCGRG	1 cut(s)	301
EcoICRI	GAGCTC	1 cut(s)	43
EcoRI	GAATTC	2 cut(s)	75, 1133
EcoRII	CCWGG	2 cut(s)	351, 1491
EcoT22I	ATGCAT	2 cut(s)	460, 1352
EcoT38I	GRGCYC	2 cut(s)	45, 193
FaeI	CATG	2 cut(s)	713, 1350
FalI	AAGNNNNNCTT	4 cut(s)	711, 743, 1201, 1233
FaqI	GGGAC	2 cut(s)	96, 1432
FatI	CATG	2 cut(s)	709, 1346
FauI	CCCGC	8 cut(s)	27, 64, 126, 136, 244, 382, 435, 606
FbaI	TGATCA	2 cut(s)	213, 1306
FblI	GTMKAC	1 cut(s)	1266
FriOI	GRGCYC	2 cut(s)	45, 193
FspBI	CTAG	6 cut(s)	431, 738, 1127, 1359, 1376, 1498
GlaI	GCGC	1 cut(s)	350
GsaI	CCCAGC	3 cut(s)	34, 595, 907
GsuI	CTGGAG	2 cut(s)	975, 1356
HaeII	RGCGCY	1 cut(s)	352
HaeIII	GGCC	4 cut(s)	191, 590, 965, 1092
HapII	CCGG	7 cut(s)	282, 302, 330, 341, 440, 638, 650
HhaI	GCGC	1 cut(s)	351
Hin1II	CATG	2 cut(s)	713, 1350
Hin6I	GCGC	1 cut(s)	349
HinP1I	GCGC	1 cut(s)	349
HincII	GTYRAC	1 cut(s)	1384
HindII	GTYRAC	1 cut(s)	1384
HinfI	GANTC	2 cut(s)	914, 1441
HpaII	CCGG	7 cut(s)	282, 302, 330, 341, 440, 638, 650
Hpy166II	GTNNAC	5 cut(s)	1058, 1267, 1366, 1384, 1455
Hpy188I	TCNGA	5 cut(s)	213, 388, 919, 1201, 1276
Hpy188III	TCNNGA	6 cut(s)	282, 440, 794, 991, 1294, 1445
Hpy8I	GTNNAC	5 cut(s)	1058, 1267, 1366, 1384, 1455
HpyAV	CCTTC	2 cut(s)	305, 655
HpyCH4III	ACNGT	1 cut(s)	757
HpyCH4V	TGCA	5 cut(s)	446, 458, 878, 1205, 1350
HpyF10VI	GCNNNNNNNGC	2 cut(s)	40, 374
HpyF3I	CTNAG	5 cut(s)	126, 204, 1292, 1422, 1470
Hsp92II	CATG	2 cut(s)	713, 1350
HspAI	GCGC	1 cut(s)	349
Kpn2I	TCCGGA	2 cut(s)	281, 439
Ksp22I	TGATCA	2 cut(s)	213, 1306
Kzo9I	GATC	6 cut(s)	106, 208, 213, 284, 871, 1306
LmnI	GCTCC	7 cut(s)	40, 48, 164, 492, 561, 1331, 1471
LweI	GCATC	5 cut(s)	433, 467, 1030, 1192, 1438
MaeI	CTAG	6 cut(s)	431, 738, 1127, 1359, 1376, 1498
MaeIII	GTNAC	2 cut(s)	470, 1429
MalI	GATC	6 cut(s)	108, 210, 215, 286, 873, 1308
MboI	GATC	6 cut(s)	106, 208, 213, 284, 871, 1306
MboII	GAAGA	3 cut(s)	520, 900, 1138
MfeI	CAATTG	1 cut(s)	217
MflI	RGATCY	3 cut(s)	106, 208, 284
MhlI	GDGCHC	5 cut(s)	45, 193, 375, 504, 1199
MluCI	AATT	6 cut(s)	75, 217, 803, 891, 1074, 1133
MlyI	GAGTC	2 cut(s)	923, 1450
MmeI	TCCRAC	3 cut(s)	411, 657, 739
MnlI	CCTC	8 cut(s)	332, 533, 1073, 1123, 1225, 1301, 1331, 1417
Mph1103I	ATGCAT	2 cut(s)	460, 1352
MroI	TCCGGA	2 cut(s)	281, 439
MseI	TTAA	1 cut(s)	411
MslI	CAYNNNNRTG	1 cut(s)	1401
MspA1I	CMGCKG	1 cut(s)	34
MspI	CCGG	7 cut(s)	282, 302, 330, 341, 440, 638, 650
MspR9I	CCNGG	7 cut(s)	302, 303, 341, 353, 638, 650, 1493
MunI	CAATTG	1 cut(s)	217
Mva1269I	GAATGC	3 cut(s)	460, 638, 1468
MvaI	CCWGG	2 cut(s)	353, 1493
MvnI	CGCG	1 cut(s)	368
MwoI	GCNNNNNNNGC	2 cut(s)	40, 374
NciI	CCSGG	5 cut(s)	302, 303, 341, 638, 650
NdeII	GATC	6 cut(s)	106, 208, 213, 284, 871, 1306
NlaIII	CATG	2 cut(s)	713, 1350
NlaIV	GGNNCC	5 cut(s)	108, 191, 286, 372, 438
NmuCI	GTSAC	2 cut(s)	470, 1429
NsiI	ATGCAT	2 cut(s)	460, 1352
NspI	RCATGY	2 cut(s)	713, 1350
PctI	GAATGC	3 cut(s)	460, 638, 1468
PleI	GAGTC	2 cut(s)	922, 1449
PpsI	GAGTC	2 cut(s)	922, 1449
Psp124BI	GAGCTC	1 cut(s)	45
Psp6I	CCWGG	2 cut(s)	351, 1491
PspFI	CCCAGC	3 cut(s)	30, 591, 903
PspGI	CCWGG	2 cut(s)	351, 1491
PspN4I	GGNNCC	5 cut(s)	108, 191, 286, 372, 438
PspOMI	GGGCCC	1 cut(s)	189
PspPI	GGNCC	7 cut(s)	4, 189, 190, 279, 355, 589, 1091
PsuI	RGATCY	3 cut(s)	106, 208, 284
RsaI	GTAC	2 cut(s)	152, 1235
RsaNI	GTAC	2 cut(s)	151, 1234
RseI	CAYNNNNRTG	1 cut(s)	1401
SacI	GAGCTC	1 cut(s)	45
SaqAI	TTAA	1 cut(s)	411
Sau3AI	GATC	6 cut(s)	106, 208, 213, 284, 871, 1306
Sau96I	GGNCC	7 cut(s)	4, 189, 190, 279, 355, 589, 1091
SchI	GAGTC	2 cut(s)	923, 1450
ScrFI	CCNGG	7 cut(s)	302, 303, 341, 353, 638, 650, 1493
SduI	GDGCHC	5 cut(s)	45, 193, 375, 504, 1199
SfaNI	GCATC	5 cut(s)	433, 467, 1030, 1192, 1438
SinI	GGWCC	3 cut(s)	4, 279, 355
SmaI	CCCGGG	1 cut(s)	303
SmiMI	CAYNNNNRTG	1 cut(s)	1401
SmlI	CTYRAG	1 cut(s)	1167
SmoI	CTYRAG	1 cut(s)	1167
Sse9I	AATT	6 cut(s)	75, 217, 803, 891, 1074, 1133
SspI	AATATT	1 cut(s)	1487
SspMI	CTAG	6 cut(s)	431, 738, 1127, 1359, 1376, 1498
SstI	GAGCTC	1 cut(s)	45
StyD4I	CCNGG	7 cut(s)	300, 301, 339, 351, 636, 648, 1491
TaaI	ACNGT	1 cut(s)	757
TaqI	TCGA	1 cut(s)	1446
TasI	AATT	6 cut(s)	75, 217, 803, 891, 1074, 1133
TatI	WGTACW	2 cut(s)	150, 1233
Tru1I	TTAA	1 cut(s)	411
Tru9I	TTAA	1 cut(s)	411
TscAI	CASTG	1 cut(s)	509
TseFI	GTSAC	2 cut(s)	470, 1429
Tsp45I	GTSAC	2 cut(s)	470, 1429
TspDTI	ATGAA	4 cut(s)	467, 645, 1475, 1493
TspGWI	ACGGA	1 cut(s)	458
TspMI	CCCGGG	1 cut(s)	301
TspRI	CASTG	1 cut(s)	509
VpaK11BI	GGWCC	3 cut(s)	4, 279, 355
XapI	RAATTY	3 cut(s)	75, 891, 1133
XceI	RCATGY	2 cut(s)	713, 1350
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1052
XmaI	CCCGGG	1 cut(s)	301
XmiI	GTMKAC	1 cut(s)	1266
XspI	CTAG	6 cut(s)	431, 738, 1127, 1359, 1376, 1498
Zsp2I	ATGCAT	2 cut(s)	460, 1352

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.