Rorug03G0140300

Belongs to the glycosyl hydrolase 32 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002984
Physical Location & Seq
Forward (+)
11534490 .. 11537129
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0140300.1

Sequence Viewer

Length: 2046 bp
ATGGGGAACGCCATCGCAAAGGTCTCTCACTGCTGCTCTGTCGCCGGCGAAGTCTCCAGGCGCCACGACGTCGACGTCCTCTCCCCCGACGAGGGTCTCGGCCACTCCTTCTACTACATTCGCCGTCCAGAAATAATCTCCACCACGAACTCGTTTGAAGCTTTCTCCGAGGAGTTCCCCAACCCGGCTACCACCACGTTCCGGTCAATCTCCGGCGCCTCCGTCAGCGCCAACACGTTCACGCCGCTGTCTACTACGCTATATGATCTCTACCCCGACATCTCCGGTGTTCTCGATCGTGCTGCTAGGTTTGAGAGCTCTACTTTGTTCGCTTCGATTCCTCTGCAGCCGGTTCCTCGGAACCCGGGACTTCCGGGGTCCGGTCCTGCTGAAAGAGGCTTCTTGTCCGGTCCGATCGAGCGGGGGTTCATGTCCGGTCCGATTGACCGGTTGGTGTGTTCGGGGCCGATTGAGAAGCAGTCCGAGAAGATGGAGAGAAGCCTCTCTTTTGGGCTTGAGCAAAAGCCCAAGAGAAGGAATTTGATGAGGATGATAAGAAGAGCGATTTCGAAGAGTATGAATCGGCGGCACAAGTCGGCTGTGACGCCGAAAGAAACGGAGTCGGAGAAAATCGGCGAGGAGTTGAATCCGAGTAGTACTAACTTGAGCAGCCATGCGAGTTTGAGTGGAAATGAGAATGGGATTGGGGATTTCTCAATGGAAAGTCAGAATCTTCAGTGGGCTCAGGGGAAGGCGGGTGAGGATCGAGTTCATGTTGTGGTTTCTGATGAGCATGGTTGGGTGTTTGTGGGGATTTATGATGGGTTTAATGGCCCTGATGCTCCTGATTATTTGTTGTCGAATCTTTACTCTGCTGTGCATGAGGAGCTCAAGGGGTTGCTGTGGAGTGACATGTTGGAAATCGAATCCACGGAATTGAAATCATCGGATTCCAATGTGGTTTCGTGTTCTTTTAGTGAGGTTGGCTGCATTGATGGAGTTGATAAGGAGAATTGTACAGTGGAGAATGTGTTGTCAAATAGAAAACAGAGCAGGCATTTGAGGAATGAAAGTAATGAGATGGATAATAAGTGGGAGGAGAGTCAGAGGAGATGGAAGTGTGAATGGGATAGAGAAAGGCCAGAGCTTGATAGGTTAACTAAGGAGAACTTGAAGCTTGAGGTGTCGAATGGTGTGGCAGCTGCAAACCCCTCGGATGTTCTCAAGGCTCTTTCGGAGGCGTTAAGAAAAACTGAGGAGGCCTATTTGGATGTTGCGGATAAGATGGTCACCGAAAATCCTGAGTTGGCTCTGATGGGGTCTTGTGTGCTGGTGATGTTGATGAAGGGAGATGATGTTTACTTGATGAATGTTGGGGATAGTCGTGCGGTTTTAGCTCAGAAAGTCGATCCCAAAATCAAGCGAAACTTGGAACGGATTAATGAGGAAGCTTTATGCGAACGTCGATCATCTAGTGCTGATGAGCTTTACAGTTTAAACAATTTGACTTCGGTTCAGCTGACAATGGATCACAGTACAAGTGTGAAAGAGGAAGTTCGAAGAATAAGGAATGAACATCCAGATGATGCTACTGCAGTGATGAATAAGAGAGTGAAGGGTTATTTGAAGGTCACTCGGGCATTTGGTGCTGGCTTTCTAAAACAGCCAAAATGGAATGATGCACTGCTAGAGATGTTTAGACTCAATTATGTTGGGACTGCCCCCTACATTACATGTATACCATCACTGTATCACCACAAACTCAGACCAAGAGACAGACTCTTAATATTGTCCTCTGATGGACTCTATCAGTACTTCACCAATGAAGAAGCCGTCTCCGAAGTAGAGTTGTTCATCTCTTCCTTTCCTGATGGTGACCCTGCACAACATCTGATTGAAGAAGTGTTGTTTAGAGCAGCAAAGAAAGCTGGACTGGCCTTCCATGAACTGCTTGACATCCCACAAGGCGAACGTCGTCGGTACCATGATGACATTTCCATTCTTATTATTTCACTTGAGGGAAGAATATGGCGTTCATCGCTTTGA

Protein Analysis

681

Amino Acids

76.4

Weight (kDa)

5.52

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 403 - 623 5.2e-27 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 2 cut(s) 72, 78
Acc65I GGTACC 1 cut(s) 1980
AccB1I GGYRCC 3 cut(s) 60, 215, 1980
AccBSI CCGCTC 1 cut(s) 421
AccI GTMKAC 3 cut(s) 72, 251, 1738
AciI CCGC 6 cut(s) 245, 421, 586, 755, 1277, 1388
AclWI GGATC 3 cut(s) 771, 1403, 1536
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 1 cut(s) 719
AcyI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
AfaI GTAC 5 cut(s) 658, 1018, 1537, 1814, 1982
AfiI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
AflIII ACRYGT 3 cut(s) 234, 912, 1733
AgeI ACCGGT 1 cut(s) 447
AgsI TTSAA 6 cut(s) 158, 646, 940, 1174, 1627, 1898
AjnI CCWGG 1 cut(s) 56
Alw21I GWGCWC 2 cut(s) 320, 891
Alw26I GTCTC 5 cut(s) 28, 58, 101, 1767, 1840
AlwI GGATC 3 cut(s) 771, 1403, 1536
Ama87I CYCGRG 2 cut(s) 364, 1635
AoxI GGCC 6 cut(s) 100, 464, 832, 1139, 1260, 1935
ApeKI GCWGC 8 cut(s) 33, 302, 346, 669, 987, 1199, 1202, 1916
ApoI RAATTY 1 cut(s) 538
AseI ATTAAT 1 cut(s) 1440
AsiGI ACCGGT 1 cut(s) 447
Asp718I GGTACC 1 cut(s) 1980
AspLEI GCGC 3 cut(s) 63, 218, 230
AspS9I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
AsuC2I CCSGG 4 cut(s) 185, 365, 366, 375
AsuHPI GGTGA 6 cut(s) 770, 1282, 1345, 1745, 1810, 1886
AsuII TTCGAA 2 cut(s) 569, 1558
AvaI CYCGRG 2 cut(s) 364, 1635
AvaII GGWCC 4 cut(s) 378, 383, 410, 437
BanI GGYRCC 3 cut(s) 60, 215, 1980
BanII GRGCYC 3 cut(s) 320, 745, 891
Bbv12I GWGCWC 2 cut(s) 320, 891
BbvI GCAGC 8 cut(s) 20, 289, 358, 681, 974, 1189, 1211, 1928
BceAI ACGGC 2 cut(s) 108, 1817
BcgI CGANNNNNNTGC 6 cut(s) 284, 318, 325, 359, 1194, 1228
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 4 cut(s) 185, 365, 366, 375
BcoDI GTCTC 5 cut(s) 28, 58, 101, 1767, 1840
BfaI CTAG 3 cut(s) 306, 1473, 1688
BfmI CTRYAG 2 cut(s) 344, 1593
BfoI RGCGCY 3 cut(s) 64, 219, 231
BmcAI AGTACT 2 cut(s) 658, 1814
Bme1390I CCNGG 5 cut(s) 58, 185, 365, 366, 375
Bme18I GGWCC 4 cut(s) 378, 383, 410, 437
BmeT110I CYCGRG 2 cut(s) 364, 1635
BmgT120I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
BmiI GGNNCC 7 cut(s) 62, 217, 354, 362, 379, 465, 1982
BmrFI CCNGG 5 cut(s) 58, 185, 365, 366, 375
BmsI GCATC 3 cut(s) 829, 1576, 1669
BoxI GACNNNNGTC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 1764, 1796
BpmI CTGGAG 1 cut(s) 40
Bpu10I CCTNAGC 1 cut(s) 744
Bpu14I TTCGAA 2 cut(s) 569, 1558
BpuEI CTTGAG 6 cut(s) 536, 685, 875, 1199, 1208, 2036
BpuMI CCSGG 4 cut(s) 185, 365, 366, 375
BsaHI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
BsaI GGTCTC 2 cut(s) 28, 101
BsaJI CCNNGG 6 cut(s) 168, 356, 364, 374, 930, 1212
BsaWI WCCGGW 6 cut(s) 201, 284, 380, 407, 434, 447
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 1016, 1046, 1341, 1371
Bsc4I CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
Bse118I RCCGGY 3 cut(s) 44, 349, 447
Bse1I ACTGG 1 cut(s) 1938
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 6 cut(s) 168, 356, 364, 374, 930, 1212
BseGI GGATG 5 cut(s) 555, 1222, 1276, 1576, 1956
BseLI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
BseMII CTCAG 5 cut(s) 758, 1245, 1293, 1412, 1777
BseNI ACTGG 1 cut(s) 1938
BseRI GAGGAG 6 cut(s) 185, 653, 899, 1112, 1123, 1271
BseXI GCAGC 8 cut(s) 20, 289, 358, 681, 974, 1189, 1211, 1928
BsgI GTGCAG 1 cut(s) 1866
Bsh1285I CGRYCG 2 cut(s) 298, 417
BshFI GGCC 6 cut(s) 102, 466, 834, 1141, 1262, 1937
BshNI GGYRCC 3 cut(s) 60, 215, 1980
BshTI ACCGGT 1 cut(s) 447
BsiEI CGRYCG 2 cut(s) 298, 417
BsiHKAI GWGCWC 2 cut(s) 320, 891
BsiHKCI CYCGRG 2 cut(s) 364, 1635
BslFI GGGAC 2 cut(s) 381, 1729
BslI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
BsmAI GTCTC 5 cut(s) 28, 58, 101, 1767, 1840
BsmBI CGTCTC 1 cut(s) 1840
BsmFI GGGAC 2 cut(s) 381, 1729
BsnI GGCC 6 cut(s) 102, 466, 834, 1141, 1262, 1937
Bso31I GGTCTC 2 cut(s) 28, 101
BsoBI CYCGRG 2 cut(s) 364, 1635
Bsp119I TTCGAA 2 cut(s) 569, 1558
Bsp1286I GDGCHC 3 cut(s) 320, 745, 891
Bsp1407I TGTACA 1 cut(s) 1016
Bsp143I GATC 7 cut(s) 265, 295, 414, 763, 1408, 1466, 1528
BspACI CCGC 6 cut(s) 245, 421, 586, 755, 1277, 1388
BspANI GGCC 6 cut(s) 102, 466, 834, 1141, 1262, 1937
BspCNI CTCAG 5 cut(s) 757, 1246, 1294, 1411, 1776
BspLI GGNNCC 7 cut(s) 62, 217, 354, 362, 379, 465, 1982
BspMAI CTGCAG 2 cut(s) 348, 1597
BspPI GGATC 3 cut(s) 771, 1403, 1536
BspQI GCTCTTC 1 cut(s) 553
BspT104I TTCGAA 2 cut(s) 569, 1558
BspT107I GGYRCC 3 cut(s) 60, 215, 1980
BspTNI GGTCTC 2 cut(s) 28, 101
BsrBI CCGCTC 1 cut(s) 421
BsrFI RCCGGY 3 cut(s) 44, 349, 447
BsrGI TGTACA 1 cut(s) 1016
BsrI ACTGG 1 cut(s) 1938
BssAI RCCGGY 3 cut(s) 44, 349, 447
BssECI CCNNGG 6 cut(s) 168, 356, 364, 374, 930, 1212
BssMI GATC 7 cut(s) 265, 295, 414, 763, 1408, 1466, 1528
BssNAI GTATAC 1 cut(s) 1739
BssNI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
Bst1107I GTATAC 1 cut(s) 1739
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 4 cut(s) 1021, 1493, 1535, 1749
Bst6I CTCTTC 3 cut(s) 553, 566, 1864
BstACI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
BstAPI GCANNNNNTGC 1 cut(s) 1646
BstAUI TGTACA 1 cut(s) 1016
BstBI TTCGAA 2 cut(s) 569, 1558
BstC8I GCNNGC 3 cut(s) 46, 1055, 1651
BstDEI CTNAG 6 cut(s) 744, 1161, 1254, 1302, 1398, 1763
BstDSI CCRYGG 1 cut(s) 930
BstEII GGTNACC 2 cut(s) 1288, 1874
BstF5I GGATG 5 cut(s) 555, 1222, 1276, 1576, 1956
BstH2I RGCGCY 3 cut(s) 64, 219, 231
BstHHI GCGC 3 cut(s) 63, 218, 230
BstKTI GATC 7 cut(s) 268, 298, 417, 766, 1411, 1469, 1531
BstMAI GTCTC 5 cut(s) 28, 58, 101, 1767, 1840
BstMBI GATC 7 cut(s) 265, 295, 414, 763, 1408, 1466, 1528
BstMCI CGRYCG 2 cut(s) 298, 417
BstMWI GCNNNNNNNGC 6 cut(s) 886, 1394, 1646, 1925, 1934, 2038
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 2 cut(s) 916, 1737
BstPAI GACNNNNGTC 1 cut(s) 93
BstPI GGTNACC 2 cut(s) 1288, 1874
BstSCI CCNGG 5 cut(s) 56, 183, 363, 364, 373
BstSFI CTRYAG 2 cut(s) 344, 1593
BstV1I GCAGC 8 cut(s) 20, 289, 358, 681, 974, 1189, 1211, 1928
BstZ17I GTATAC 1 cut(s) 1739
BsuRI GGCC 6 cut(s) 102, 466, 834, 1141, 1262, 1937
BtgI CCRYGG 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 2022
BtsCI GGATG 5 cut(s) 555, 1222, 1276, 1576, 1956
BtsI GCAGTG 3 cut(s) 28, 1602, 1682
BtsIMutI CAGTG 6 cut(s) 28, 743, 1026, 1602, 1682, 1745
Cac8I GCNNGC 3 cut(s) 46, 1055, 1651
CfoI GCGC 3 cut(s) 63, 218, 230
Cfr10I RCCGGY 3 cut(s) 44, 349, 447
Cfr13I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
Cfr9I CCCGGG 1 cut(s) 364
CpoI CGGWCCG 2 cut(s) 410, 437
CseI GACGC 1 cut(s) 613
Csp6I GTAC 5 cut(s) 657, 1017, 1536, 1813, 1981
CspAI ACCGGT 1 cut(s) 447
CspI CGGWCCG 2 cut(s) 410, 437
CviAII CATG 9 cut(s) 430, 674, 773, 794, 881, 913, 1734, 1943, 1985
CviQI GTAC 5 cut(s) 657, 1017, 1536, 1813, 1981
DdeI CTNAG 6 cut(s) 744, 1161, 1254, 1302, 1398, 1763
DinI GGCGCC 2 cut(s) 62, 217
DpnI GATC 7 cut(s) 267, 297, 416, 765, 1410, 1468, 1530
DpnII GATC 7 cut(s) 265, 295, 414, 763, 1408, 1466, 1528
DraI TTTAAA 1 cut(s) 1497
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 3 cut(s) 553, 566, 1864
EarI CTCTTC 3 cut(s) 553, 566, 1864
Ecl136II GAGCTC 2 cut(s) 318, 889
Eco147I AGGCCT 1 cut(s) 1262
Eco24I GRGCYC 3 cut(s) 320, 745, 891
Eco31I GGTCTC 2 cut(s) 28, 101
Eco47I GGWCC 4 cut(s) 378, 383, 410, 437
Eco53kI GAGCTC 2 cut(s) 318, 889
Eco57I CTGAAG 1 cut(s) 719
Eco88I CYCGRG 2 cut(s) 364, 1635
Eco91I GGTNACC 2 cut(s) 1288, 1874
EcoICRI GAGCTC 2 cut(s) 318, 889
EcoO65I GGTNACC 2 cut(s) 1288, 1874
EcoRII CCWGG 1 cut(s) 56
EcoT38I GRGCYC 3 cut(s) 320, 745, 891
EgeI GGCGCC 2 cut(s) 62, 217
EheI GGCGCC 2 cut(s) 62, 217
Esp3I CGTCTC 1 cut(s) 1840
FaeI CATG 9 cut(s) 433, 677, 776, 797, 884, 916, 1737, 1946, 1988
FalI AAGNNNNNCTT 6 cut(s) 490, 522, 1154, 1186, 1412, 1444
FaqI GGGAC 2 cut(s) 381, 1729
FatI CATG 9 cut(s) 429, 673, 772, 793, 880, 912, 1733, 1942, 1984
FauI CCCGC 2 cut(s) 414, 748
FblI GTMKAC 3 cut(s) 72, 251, 1738
FokI GGATG 5 cut(s) 562, 1229, 1283, 1563, 1943
FriOI GRGCYC 3 cut(s) 320, 745, 891
FspBI CTAG 3 cut(s) 306, 1473, 1688
GlaI GCGC 3 cut(s) 62, 217, 229
GsuI CTGGAG 1 cut(s) 40
HaeII RGCGCY 3 cut(s) 64, 219, 231
HaeIII GGCC 6 cut(s) 102, 466, 834, 1141, 1262, 1937
HgaI GACGC 1 cut(s) 613
HhaI GCGC 3 cut(s) 63, 218, 230
Hin1I GRCGYC 5 cut(s) 61, 69, 75, 216, 605
Hin1II CATG 9 cut(s) 433, 677, 776, 797, 884, 916, 1737, 1946, 1988
Hin6I GCGC 3 cut(s) 61, 216, 228
HinP1I GCGC 3 cut(s) 61, 216, 228
HincII GTYRAC 2 cut(s) 73, 1158
HindII GTYRAC 2 cut(s) 73, 1158
HindIII AAGCTT 3 cut(s) 159, 1175, 1449
HpaI GTTAAC 1 cut(s) 1158
HphI GGTGA 6 cut(s) 770, 1282, 1345, 1745, 1810, 1886
Hpy166II GTNNAC 6 cut(s) 73, 240, 252, 1158, 1360, 1739
Hpy188III TCNNGA 6 cut(s) 128, 293, 845, 1301, 1580, 1868
Hpy8I GTNNAC 6 cut(s) 73, 240, 252, 1158, 1360, 1739
Hpy99I CGWCG 7 cut(s) 71, 74, 77, 92, 1467, 1977, 1980
HpyAV CCTTC 7 cut(s) 118, 528, 745, 1339, 1609, 1621, 1948
HpyCH4III ACNGT 4 cut(s) 1021, 1493, 1535, 1749
HpyCH4IV ACGT 6 cut(s) 69, 75, 197, 236, 1462, 1972
HpyCH4V TGCA 7 cut(s) 346, 880, 990, 1205, 1595, 1682, 1883
HpyF10VI GCNNNNNNNGC 6 cut(s) 886, 1394, 1646, 1925, 1934, 2038
HpyF3I CTNAG 6 cut(s) 744, 1161, 1254, 1302, 1398, 1763
HpySE526I ACGT 6 cut(s) 69, 75, 197, 236, 1462, 1972
Hsp92I GRCGYC 5 cut(s) 61, 69, 75, 216, 605
Hsp92II CATG 9 cut(s) 433, 677, 776, 797, 884, 916, 1737, 1946, 1988
HspAI GCGC 3 cut(s) 61, 216, 228
KasI GGCGCC 2 cut(s) 60, 215
KpnI GGTACC 1 cut(s) 1984
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
KspAI GTTAAC 1 cut(s) 1158
Kzo9I GATC 7 cut(s) 265, 295, 414, 763, 1408, 1466, 1528
LguI GCTCTTC 1 cut(s) 553
LmnI GCTCC 2 cut(s) 847, 886
Lsp1109I GCAGC 8 cut(s) 20, 289, 358, 681, 974, 1189, 1211, 1928
LweI GCATC 3 cut(s) 829, 1576, 1669
MaeI CTAG 3 cut(s) 306, 1473, 1688
MaeII ACGT 6 cut(s) 69, 75, 197, 236, 1462, 1972
MaeIII GTNAC 5 cut(s) 601, 908, 1288, 1630, 1874
MalI GATC 7 cut(s) 267, 297, 416, 765, 1410, 1468, 1530
MbiI CCGCTC 1 cut(s) 421
MboI GATC 7 cut(s) 265, 295, 414, 763, 1408, 1466, 1528
MboII GAAGA 9 cut(s) 499, 570, 583, 725, 1572, 1838, 1851, 1910, 2034
MhlI GDGCHC 3 cut(s) 320, 745, 891
MluCI AATT 5 cut(s) 538, 935, 1012, 1501, 1705
Mly113I GGCGCC 2 cut(s) 61, 216
MlyI GAGTC 5 cut(s) 629, 1111, 1695, 1773, 1797
MmeI TCCRAC 2 cut(s) 603, 897
MreI CGCCGGCG 1 cut(s) 44
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 6 cut(s) 828, 1157, 1244, 1440, 1496, 1784
MslI CAYNNNNRTG 1 cut(s) 1581
MspA1I CMGCKG 3 cut(s) 247, 1202, 1519
MspR9I CCNGG 5 cut(s) 58, 185, 365, 366, 375
MssI GTTTAAAC 1 cut(s) 1497
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 6 cut(s) 886, 1394, 1646, 1925, 1934, 2038
NaeI GCCGGC 1 cut(s) 46
NarI GGCGCC 2 cut(s) 61, 216
NciI CCSGG 4 cut(s) 185, 365, 366, 375
NdeII GATC 7 cut(s) 265, 295, 414, 763, 1408, 1466, 1528
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 9 cut(s) 433, 677, 776, 797, 884, 916, 1737, 1946, 1988
NlaIV GGNNCC 7 cut(s) 62, 217, 354, 362, 379, 465, 1982
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 5 cut(s) 601, 908, 1288, 1630, 1874
NspI RCATGY 2 cut(s) 916, 1737
NspV TTCGAA 2 cut(s) 569, 1558
PceI AGGCCT 1 cut(s) 1262
PciI ACATGT 2 cut(s) 912, 1733
PciSI GCTCTTC 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 7 cut(s) 337, 580, 646, 730, 862, 926, 950
PinAI ACCGGT 1 cut(s) 447
Ple19I CGATCG 2 cut(s) 298, 417
PleI GAGTC 5 cut(s) 628, 1110, 1695, 1773, 1797
PluTI GGCGCC 2 cut(s) 64, 219
PmeI GTTTAAAC 1 cut(s) 1497
PpsI GAGTC 5 cut(s) 628, 1110, 1695, 1773, 1797
PscI ACATGT 2 cut(s) 912, 1733
PshAI GACNNNNGTC 1 cut(s) 93
PshBI ATTAAT 1 cut(s) 1440
Psp124BI GAGCTC 2 cut(s) 320, 891
Psp6I CCWGG 1 cut(s) 56
PspEI GGTNACC 2 cut(s) 1288, 1874
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 7 cut(s) 62, 217, 354, 362, 379, 465, 1982
PspPI GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
PstI CTGCAG 2 cut(s) 348, 1597
PvuI CGATCG 2 cut(s) 298, 417
PvuII CAGCTG 2 cut(s) 1202, 1519
RsaI GTAC 5 cut(s) 658, 1018, 1537, 1814, 1982
RsaNI GTAC 5 cut(s) 657, 1017, 1536, 1813, 1981
RseI CAYNNNNRTG 1 cut(s) 1581
Rsr2I CGGWCCG 2 cut(s) 410, 437
RsrII CGGWCCG 2 cut(s) 410, 437
SacI GAGCTC 2 cut(s) 320, 891
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 553
SaqAI TTAA 6 cut(s) 828, 1157, 1244, 1440, 1496, 1784
Sau3AI GATC 7 cut(s) 265, 295, 414, 763, 1408, 1466, 1528
Sau96I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
ScaI AGTACT 2 cut(s) 658, 1814
SchI GAGTC 5 cut(s) 629, 1111, 1695, 1773, 1797
ScrFI CCNGG 5 cut(s) 58, 185, 365, 366, 375
SduI GDGCHC 3 cut(s) 320, 745, 891
SfaNI GCATC 3 cut(s) 829, 1576, 1669
SfcI CTRYAG 2 cut(s) 344, 1593
SfoI GGCGCC 2 cut(s) 62, 217
SfuI TTCGAA 2 cut(s) 569, 1558
SgrAI CRCCGGYG 1 cut(s) 44
SgrDI CGTCGACG 1 cut(s) 71
SinI GGWCC 4 cut(s) 378, 383, 410, 437
SmaI CCCGGG 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 1581
SmlI CTYRAG 6 cut(s) 515, 664, 890, 1178, 1223, 2015
SmoI CTYRAG 6 cut(s) 515, 664, 890, 1178, 1223, 2015
Sse9I AATT 5 cut(s) 538, 935, 1012, 1501, 1705
SseBI AGGCCT 1 cut(s) 1262
SsiI CCGC 6 cut(s) 245, 421, 586, 755, 1277, 1388
SspDI GGCGCC 2 cut(s) 60, 215
SspI AATATT 1 cut(s) 1788
SspMI CTAG 3 cut(s) 306, 1473, 1688
SstI GAGCTC 2 cut(s) 320, 891
StuI AGGCCT 1 cut(s) 1262
StyD4I CCNGG 5 cut(s) 56, 183, 363, 364, 373
TaaI ACNGT 4 cut(s) 1021, 1493, 1535, 1749
TaiI ACGT 6 cut(s) 72, 78, 200, 239, 1465, 1975
TasI AATT 5 cut(s) 538, 935, 1012, 1501, 1705
TatI WGTACW 4 cut(s) 656, 1016, 1535, 1812
TauI GCSGC 2 cut(s) 247, 589
TfiI GAWTC 7 cut(s) 337, 580, 646, 730, 862, 926, 950
Tru1I TTAA 6 cut(s) 828, 1157, 1244, 1440, 1496, 1784
Tru9I TTAA 6 cut(s) 828, 1157, 1244, 1440, 1496, 1784
TscAI CASTG 6 cut(s) 35, 743, 1026, 1602, 1689, 1752
TseFI GTSAC 5 cut(s) 601, 908, 1288, 1630, 1874
TseI GCWGC 8 cut(s) 33, 302, 346, 669, 987, 1199, 1202, 1916
Tsp45I GTSAC 5 cut(s) 601, 908, 1288, 1630, 1874
TspGWI ACGGA 4 cut(s) 211, 632, 947, 1450
TspMI CCCGGG 1 cut(s) 364
TspRI CASTG 6 cut(s) 35, 743, 1026, 1602, 1689, 1752
VpaK11BI GGWCC 4 cut(s) 378, 383, 410, 437
VspI ATTAAT 1 cut(s) 1440
XapI RAATTY 1 cut(s) 538
XceI RCATGY 2 cut(s) 916, 1737
XmaI CCCGGG 1 cut(s) 364
XmiI GTMKAC 3 cut(s) 72, 251, 1738
XspI CTAG 3 cut(s) 306, 1473, 1688
ZraI GACGTC 2 cut(s) 70, 76
ZrmI AGTACT 2 cut(s) 658, 1814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.