Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0002984

Physical Location & Seq

Genomic Coordinates Forward (+)

19869472 .. 19873972

Gene Structure 4501 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG220400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGTACTTCAACGGGGTGTACCACTTATTCTACCAATACAATCCCAAAGGATCTGTGTGGGGAAACATCGTGTGGGCGCACTCCGTCTCTAAAGACCTAATCAACTGGGAAGCCCTAGAACCAGCCATATACCCCTCCAAACCCTTCGACATGAACGGTTGCTGGTCCGGATCCGCCACCATCCTCCCGGGCAACAAGCCCGTCATCCTCTACACAGGCCTCGACCCGGAAAAGCGCCAGGTCCAGAACTACGCCATCCCCAAAGACGACACCGACCCCTACCTCCGCGAATGGATCAAACCAGACAACAACCCATTGGTGGTGCCCTCCCCGGAAATGAACGTCAGCCAGTTCCGCGACCCCACCACAGCCTGGTGGTACAACGGCCACTGGAGGATGGCTGTAGGAGGAAAGAGAGGGCAGCGAGGCATGGCGTATCTGTACCGGAGCATGGACTTTAAGTTCTGGGTCAAGAGCAAGCACCCGCTCCATTCGGCTGAGAATACCGGCATGTGGGAGTGCCCGGACTTTTTCCCGTTGTATAAGAATGGGAACAAGCAGGGAGCGGACACCTCGAGAGTTGGGCCGGATGTGAAGCACGCGTTCAAGGTGAGCCTGGATGAGACGAGGTATGAGTACTACACACTGGGGATGTATAAGCCGGAAGATGACTCGTACAAAGTGGATCCGAGAATGCTGGACAGTCGGGACGGGTTGAGATATGATTATGGCAACTTTTATGCGTCCAAAACTTTCTTTGATCCTGTGAAGGGTAGAAGGATTTTGTGGGGTTGGGCTAATGAGTCTGATTCAGCTGACGAAGATACGGAAAAGGGGTGGGCCGGAATTCAGGCAATTCCACGACAGATCTGGCTCAGTCCTGATGGGAAGCAAGTGCTACAATGGCCTATCCAAGAAGTGGAAACTTTGAGGGGGCAAAAGATTGACATAACAAATCAACTTCTAGAAAAGGGAAAGCCTTTTGAAGTTCGTTCAATAACTGCTGCACAGGCCGATGTTGAAGTTACCTTCACCATTCCGAGCGTGGACAAAGCTGAGGAGTATGATCCTACCTGGCGGGATGCGGAAACGGCGTGTGCGGCAAAGCGTTCGAATGTTCCAGGTGGTGTTGGGCCATTCGGACTCTTGACATTGGCCTCTCAAAACCTAGAGGAGTTCACTCCCGTCTTCTTTAGGATTATCAAAATAAAAGAGCAAAAGTACAAGGTTCTTATGTGCTCTGACGCAAAGTTTTCAACTTTGAAGCCGTTCGATGAGAAACAGTATAGACCATCGTTTGCCGGCTTAGTAGATGTAGATATAGCCGCCGACAAGAAGATTTCCCTCAGGACTTTGATTGATCATTCTGCTGTTGAAAGTTTTGGAGCAGGAGGAAGAACATGCATCTTATCTAGGGTTTATCCGACCCTAGCTGTAAACGATGCAGCTCACTTGTTTTTGTTCAACAATGGCACCGAGCCAATTACTGTTCAGACTCTCACTGCTTGGAGTATGAATGCTCCTAAGGCCATGAACCAGGGCGAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0030312 GO:0033036 GO:0042742 GO:0043207 GO:0044464 GO:0050832 GO:0050896 GO:0051179 GO:0051704 GO:0051707 GO:0052575 GO:0052576 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

Glyco_hydro_32C Glyco_hydro_32N

Protein Analysis

516

Amino Acids

58.7

Weight (kDa)

6.3

Isoelectric Point (pI)

31.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_32N	PF00251	1 - 303	2.6e-95	Glycosyl hydrolases family 32 N-terminal domain
Glyco_hydro_32C	PF08244	306 - 504	2.5e-23	Glycosyl hydrolases family 32 C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36190 AT3G52600 AT3G52600 AT3G52600
fragaria_vesca	FvH4_6g19270 FvH4_6g19270 FvH4_6g19271
malus_domestica	MD12G1028200.v1.1 MD14G1028500.v1.1
prunus_persica	Prupe.7G103100_v2.0.a1 Prupe.7G103200_v2.0.a1 Prupe.7G103300_v2.0.a1 Prupe.7G103400_v2.0.a1 Prupe.7G103400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0474371 RchiOBHm_Chr3g0474381
rosa_laevigata	RLG00000023930
rosa_multiflora	Rmu_co8392863.1_g000001 Rmu_co8415991.1_g000001 Rmu_co8444851.1_g000001 Rmu_sc0004507.1_g000003 Rmu_sc0004507.1_g000004
rosa_roxburghii	Rroxscaffold_6G00407310 Rroxscaffold_6G00407320
rosa_rugosa	Rorug03G0140200 Rorug03G0140300 Rorug03G0140400 Rorug03G0140500
rosa_samantha	Rh3AG191100 Rh3AG191200 Rh3BG220300 Rh3BG220400 Rh3CG215600 Rh3CG215700 Rh3DG216000 Rh3DG216100
rosa_wichuraiana	Rw3G017430 Rw3G017440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	322, 1472
AccB7I	CCANNNNNTGG	2 cut(s)	372, 919
AccBSI	CCGCTC	2 cut(s)	487, 566
AccII	CGCG	3 cut(s)	288, 357, 602
AccIII	TCCGGA	1 cut(s)	167
AciI	CCGC	9 cut(s)	174, 286, 355, 485, 566, 1078, 1085, 1100, 1326
AclWI	GGATC	8 cut(s)	58, 165, 178, 302, 680, 693, 755, 1061
AcoI	YGGCCR	1 cut(s)	385
AcsI	RAATTY	1 cut(s)	846
AfaI	GTAC	7 cut(s)	5, 20, 380, 443, 638, 677, 1223
AfiI	CCNNNNNNNGG	7 cut(s)	226, 319, 372, 451, 513, 770, 919
AflIII	ACRYGT	1 cut(s)	600
AgsI	TTSAA	9 cut(s)	10, 607, 986, 996, 1022, 1257, 1264, 1376, 1465
AjnI	CCWGG	6 cut(s)	237, 371, 615, 1073, 1120, 1536
AloI	GAACNNNNNNTCC	2 cut(s)	446, 478
AluBI	AGCT	4 cut(s)	815, 1055, 1433, 1448
AluI	AGCT	4 cut(s)	815, 1055, 1433, 1448
Alw21I	GWGCWC	1 cut(s)	1241
Alw26I	GTCTC	2 cut(s)	91, 617
AlwI	GGATC	8 cut(s)	58, 165, 178, 302, 680, 693, 755, 1061
Ama87I	CYCGRG	2 cut(s)	187, 574
Aor13HI	TCCGGA	1 cut(s)	167
AoxI	GGCC	9 cut(s)	217, 385, 584, 840, 905, 1011, 1133, 1155, 1527
ApeKI	GCWGC	3 cut(s)	421, 1004, 1445
ApoI	RAATTY	1 cut(s)	846
Asp700I	GAANNNNTTC	1 cut(s)	1268
AspLEI	GCGC	2 cut(s)	79, 237
AspS9I	GGNCC	5 cut(s)	165, 241, 584, 840, 1133
AsuC2I	CCSGG	5 cut(s)	188, 189, 227, 332, 524
AsuHPI	GGTGA	2 cut(s)	622, 1024
AsuII	TTCGAA	1 cut(s)	1112
AvaI	CYCGRG	2 cut(s)	187, 574
AvaII	GGWCC	2 cut(s)	165, 241
AxyI	CCTNAGG	2 cut(s)	1346, 1524
BaeGI	GKGCMC	2 cut(s)	327, 524
BamHI	GGATCC	2 cut(s)	170, 685
BanI	GGYRCC	2 cut(s)	322, 1472
BbsI	GAAGAC	1 cut(s)	1180
Bbv12I	GWGCWC	1 cut(s)	1241
BbvCI	CCTCAGC	1 cut(s)	1056
BbvI	GCAGC	3 cut(s)	433, 991, 1457
BccI	CCATC	5 cut(s)	188, 263, 391, 878, 1300
BceAI	ACGGC	3 cut(s)	400, 1107, 1252
BcgI	CGANNNNNNTGC	2 cut(s)	1092, 1126
BciT130I	CCWGG	6 cut(s)	239, 373, 617, 1075, 1122, 1538
BclI	TGATCA	1 cut(s)	1360
BcnI	CCSGG	5 cut(s)	188, 189, 227, 332, 524
BcoDI	GTCTC	2 cut(s)	91, 617
BfaI	CTAG	5 cut(s)	116, 965, 1169, 1413, 1430
BfmI	CTRYAG	1 cut(s)	402
BfoI	RGCGCY	1 cut(s)	238
BglII	AGATCT	1 cut(s)	867
BisI	GCNGC	5 cut(s)	422, 1005, 1101, 1326, 1446
BlsI	GCNGC	5 cut(s)	423, 1006, 1102, 1327, 1447
BmcAI	AGTACT	1 cut(s)	638
Bme18I	GGWCC	2 cut(s)	165, 241
BmeT110I	CYCGRG	2 cut(s)	187, 574
BmgT120I	GGNCC	5 cut(s)	165, 241, 584, 840, 1133
BmiI	GGNNCC	4 cut(s)	172, 324, 687, 1474
BmrI	ACTGGG	2 cut(s)	115, 656
BmsI	GCATC	3 cut(s)	1072, 1413, 1432
BmuI	ACTGGG	2 cut(s)	115, 656
BpiI	GAAGAC	1 cut(s)	1180
BpmI	CTGGAG	1 cut(s)	412
Bpu10I	CCTNAGC	1 cut(s)	1056
Bpu14I	TTCGAA	1 cut(s)	1112
BpuMI	CCSGG	5 cut(s)	188, 189, 227, 332, 524
BsaJI	CCNNGG	3 cut(s)	187, 330, 1537
BsaWI	WCCGGW	2 cut(s)	167, 444
Bsc4I	CCNNNNNNNGG	7 cut(s)	226, 319, 372, 451, 513, 770, 919
Bse118I	RCCGGY	2 cut(s)	506, 1301
Bse1I	ACTGG	4 cut(s)	110, 349, 395, 651
Bse21I	CCTNAGG	2 cut(s)	1346, 1524
BseAI	TCCGGA	1 cut(s)	167
BseBI	CCWGG	6 cut(s)	239, 373, 617, 1075, 1122, 1538
BseDI	CCNNGG	3 cut(s)	187, 330, 1537
BseGI	GGATG	8 cut(s)	180, 204, 255, 402, 595, 625, 657, 1087
BseLI	CCNNNNNNNGG	7 cut(s)	226, 319, 372, 451, 513, 770, 919
BseMII	CTCAG	4 cut(s)	489, 889, 1047, 1360
BseNI	ACTGG	4 cut(s)	110, 349, 395, 651
BseRI	GAGGAG	2 cut(s)	1073, 1187
BseSI	GKGCMC	2 cut(s)	327, 524
BseXI	GCAGC	3 cut(s)	433, 991, 1457
BsgI	GTGCAG	1 cut(s)	990
Bsh1236I	CGCG	3 cut(s)	288, 357, 602
BshFI	GGCC	9 cut(s)	219, 387, 586, 842, 907, 1013, 1135, 1157, 1529
BshNI	GGYRCC	2 cut(s)	322, 1472
BsiHKAI	GWGCWC	1 cut(s)	1241
BsiHKCI	CYCGRG	2 cut(s)	187, 574
BslFI	GGGAC	1 cut(s)	722
BslI	CCNNNNNNNGG	7 cut(s)	226, 319, 372, 451, 513, 770, 919
BsmAI	GTCTC	2 cut(s)	91, 617
BsmBI	CGTCTC	2 cut(s)	91, 617
BsmFI	GGGAC	1 cut(s)	722
BsmI	GAATGC	2 cut(s)	699, 1522
BsnI	GGCC	9 cut(s)	219, 387, 586, 842, 907, 1013, 1135, 1157, 1529
BsoBI	CYCGRG	2 cut(s)	187, 574
Bsp119I	TTCGAA	1 cut(s)	1112
Bsp1286I	GDGCHC	3 cut(s)	327, 524, 1241
Bsp13I	TCCGGA	1 cut(s)	167
Bsp143I	GATC	8 cut(s)	50, 170, 294, 685, 760, 867, 1066, 1360
BspACI	CCGC	9 cut(s)	174, 286, 355, 485, 566, 1078, 1085, 1100, 1326
BspANI	GGCC	9 cut(s)	219, 387, 586, 842, 907, 1013, 1135, 1157, 1529
BspCNI	CTCAG	4 cut(s)	490, 888, 1048, 1359
BspEI	TCCGGA	1 cut(s)	167
BspFNI	CGCG	3 cut(s)	288, 357, 602
BspLI	GGNNCC	4 cut(s)	172, 324, 687, 1474
BspPI	GGATC	8 cut(s)	58, 165, 178, 302, 680, 693, 755, 1061
BspT104I	TTCGAA	1 cut(s)	1112
BspT107I	GGYRCC	2 cut(s)	322, 1472
BsrBI	CCGCTC	2 cut(s)	487, 566
BsrFI	RCCGGY	2 cut(s)	506, 1301
BsrI	ACTGG	4 cut(s)	110, 349, 395, 651
BssAI	RCCGGY	2 cut(s)	506, 1301
BssECI	CCNNGG	3 cut(s)	187, 330, 1537
BssMI	GATC	8 cut(s)	50, 170, 294, 685, 760, 867, 1066, 1360
Bst2UI	CCWGG	6 cut(s)	239, 373, 617, 1075, 1122, 1538
Bst4CI	ACNGT	4 cut(s)	158, 704, 1284, 1489
BstBI	TTCGAA	1 cut(s)	1112
BstC8I	GCNNGC	3 cut(s)	479, 600, 1303
BstDEI	CTNAG	6 cut(s)	498, 875, 1056, 1306, 1346, 1524
BstF5I	GGATG	8 cut(s)	180, 204, 255, 402, 595, 625, 657, 1087
BstFNI	CGCG	3 cut(s)	288, 357, 602
BstH2I	RGCGCY	1 cut(s)	238
BstHHI	GCGC	2 cut(s)	79, 237
BstKTI	GATC	8 cut(s)	53, 173, 297, 688, 763, 870, 1069, 1363
BstMAI	GTCTC	2 cut(s)	91, 617
BstMBI	GATC	8 cut(s)	50, 170, 294, 685, 760, 867, 1066, 1360
BstMWI	GCNNNNNNNGC	6 cut(s)	354, 904, 1010, 1091, 1100, 1526
BstNI	CCWGG	6 cut(s)	239, 373, 617, 1075, 1122, 1538
BstNSI	RCATGY	2 cut(s)	514, 1404
BstSFI	CTRYAG	1 cut(s)	402
BstSLI	GKGCMC	2 cut(s)	327, 524
BstUI	CGCG	3 cut(s)	288, 357, 602
BstV1I	GCAGC	3 cut(s)	433, 991, 1457
BstV2I	GAAGAC	1 cut(s)	1180
BstX2I	RGATCY	4 cut(s)	50, 170, 685, 867
BstYI	RGATCY	4 cut(s)	50, 170, 685, 867
Bsu36I	CCTNAGG	2 cut(s)	1346, 1524
BsuRI	GGCC	9 cut(s)	219, 387, 586, 842, 907, 1013, 1135, 1157, 1529
BtsCI	GGATG	8 cut(s)	180, 204, 255, 402, 595, 625, 657, 1087
BtsI	GCAGTG	1 cut(s)	1500
BtsIMutI	CAGTG	3 cut(s)	388, 644, 1500
Cac8I	GCNNGC	3 cut(s)	479, 600, 1303
CfoI	GCGC	2 cut(s)	79, 237
Cfr10I	RCCGGY	2 cut(s)	506, 1301
Cfr13I	GGNCC	5 cut(s)	165, 241, 584, 840, 1133
Cfr9I	CCCGGG	1 cut(s)	187
CseI	GACGC	2 cut(s)	732, 1253
Csp6I	GTAC	7 cut(s)	4, 19, 379, 442, 637, 676, 1222
CviAII	CATG	6 cut(s)	151, 430, 451, 511, 1401, 1531
CviQI	GTAC	7 cut(s)	4, 19, 379, 442, 637, 676, 1222
DdeI	CTNAG	6 cut(s)	498, 875, 1056, 1306, 1346, 1524
DpnI	GATC	8 cut(s)	52, 172, 296, 687, 762, 869, 1068, 1362
DpnII	GATC	8 cut(s)	50, 170, 294, 685, 760, 867, 1066, 1360
EaeI	YGGCCR	1 cut(s)	385
EciI	GGCGGA	1 cut(s)	163
Eco147I	AGGCCT	1 cut(s)	219
Eco47I	GGWCC	2 cut(s)	165, 241
Eco81I	CCTNAGG	2 cut(s)	1346, 1524
Eco88I	CYCGRG	2 cut(s)	187, 574
EcoRI	GAATTC	1 cut(s)	846
EcoRII	CCWGG	6 cut(s)	237, 371, 615, 1073, 1120, 1536
EcoT22I	ATGCAT	1 cut(s)	1406
Esp3I	CGTCTC	2 cut(s)	91, 617
FaeI	CATG	6 cut(s)	154, 433, 454, 514, 1404, 1534
FaqI	GGGAC	1 cut(s)	722
FatI	CATG	6 cut(s)	150, 429, 450, 510, 1400, 1530
FauI	CCCGC	2 cut(s)	492, 1071
FbaI	TGATCA	1 cut(s)	1360
Fnu4HI	GCNGC	5 cut(s)	422, 1005, 1101, 1326, 1446
FokI	GGATG	8 cut(s)	167, 191, 242, 409, 602, 632, 664, 1094
Fsp4HI	GCNGC	5 cut(s)	422, 1005, 1101, 1326, 1446
FspBI	CTAG	5 cut(s)	116, 965, 1169, 1413, 1430
GlaI	GCGC	2 cut(s)	78, 236
GluI	GCNGC	5 cut(s)	422, 1005, 1101, 1326, 1446
GsuI	CTGGAG	1 cut(s)	412
HaeII	RGCGCY	1 cut(s)	238
HaeIII	GGCC	9 cut(s)	219, 387, 586, 842, 907, 1013, 1135, 1157, 1529
HgaI	GACGC	2 cut(s)	732, 1253
HhaI	GCGC	2 cut(s)	79, 237
Hin1II	CATG	6 cut(s)	154, 433, 454, 514, 1404, 1534
Hin6I	GCGC	2 cut(s)	77, 235
HinP1I	GCGC	2 cut(s)	77, 235
HinfI	GANTC	5 cut(s)	671, 803, 809, 1143, 1495
HphI	GGTGA	2 cut(s)	622, 1024
Hpy166II	GTNNAC	4 cut(s)	19, 1048, 1179, 1438
Hpy188I	TCNGA	7 cut(s)	690, 808, 1041, 1142, 1243, 1425, 1494
Hpy188III	TCNNGA	9 cut(s)	168, 244, 472, 576, 707, 881, 965, 1147, 1348
Hpy8I	GTNNAC	4 cut(s)	19, 1048, 1179, 1438
HpyAV	CCTTC	4 cut(s)	154, 763, 771, 1039
HpyCH4III	ACNGT	4 cut(s)	158, 704, 1284, 1489
HpyCH4IV	ACGT	1 cut(s)	342
HpyCH4V	TGCA	3 cut(s)	1007, 1404, 1445
HpyF10VI	GCNNNNNNNGC	6 cut(s)	354, 904, 1010, 1091, 1100, 1526
HpyF3I	CTNAG	6 cut(s)	498, 875, 1056, 1306, 1346, 1524
HpySE526I	ACGT	1 cut(s)	342
Hsp92II	CATG	6 cut(s)	154, 433, 454, 514, 1404, 1534
HspAI	GCGC	2 cut(s)	77, 235
Kpn2I	TCCGGA	1 cut(s)	167
KroI	GCCGGC	1 cut(s)	1301
KroNI	GCCGGC	1 cut(s)	1303
Ksp22I	TGATCA	1 cut(s)	1360
Kzo9I	GATC	8 cut(s)	50, 170, 294, 685, 760, 867, 1066, 1360
LmnI	GCTCC	5 cut(s)	447, 492, 563, 1385, 1525
Lsp1109I	GCAGC	3 cut(s)	433, 991, 1457
LweI	GCATC	3 cut(s)	1072, 1413, 1432
MaeI	CTAG	5 cut(s)	116, 965, 1169, 1413, 1430
MaeII	ACGT	1 cut(s)	342
MaeIII	GTNAC	1 cut(s)	1024
MalI	GATC	8 cut(s)	52, 172, 296, 687, 762, 869, 1068, 1362
MbiI	CCGCTC	2 cut(s)	487, 566
MboI	GATC	8 cut(s)	50, 170, 294, 685, 760, 867, 1066, 1360
MboII	GAAGA	5 cut(s)	677, 833, 1180, 1348, 1407
MflI	RGATCY	4 cut(s)	50, 170, 685, 867
MhlI	GDGCHC	3 cut(s)	327, 524, 1241
MluCI	AATT	3 cut(s)	846, 855, 1482
MluI	ACGCGT	1 cut(s)	600
MlyI	GAGTC	4 cut(s)	665, 812, 1137, 1489
MmeI	TCCRAC	1 cut(s)	1448
Mph1103I	ATGCAT	1 cut(s)	1406
MroI	TCCGGA	1 cut(s)	167
MroNI	GCCGGC	1 cut(s)	1301
MroXI	GAANNNNTTC	1 cut(s)	1268
MseI	TTAA	1 cut(s)	459
MspA1I	CMGCKG	1 cut(s)	815
Mva1269I	GAATGC	2 cut(s)	699, 1522
MvaI	CCWGG	6 cut(s)	239, 373, 617, 1075, 1122, 1538
MvnI	CGCG	3 cut(s)	288, 357, 602
MwoI	GCNNNNNNNGC	6 cut(s)	354, 904, 1010, 1091, 1100, 1526
NaeI	GCCGGC	1 cut(s)	1303
NciI	CCSGG	5 cut(s)	188, 189, 227, 332, 524
NdeII	GATC	8 cut(s)	50, 170, 294, 685, 760, 867, 1066, 1360
NgoMIV	GCCGGC	1 cut(s)	1301
NlaIII	CATG	6 cut(s)	154, 433, 454, 514, 1404, 1534
NlaIV	GGNNCC	4 cut(s)	172, 324, 687, 1474
NsiI	ATGCAT	1 cut(s)	1406
NspI	RCATGY	2 cut(s)	514, 1404
NspV	TTCGAA	1 cut(s)	1112
PaeR7I	CTCGAG	1 cut(s)	574
PceI	AGGCCT	1 cut(s)	219
PctI	GAATGC	2 cut(s)	699, 1522
PdiI	GCCGGC	1 cut(s)	1303
PdmI	GAANNNNTTC	1 cut(s)	1268
PfeI	GAWTC	1 cut(s)	809
PflMI	CCANNNNNTGG	2 cut(s)	372, 919
PkrI	GCNGC	5 cut(s)	423, 1006, 1102, 1327, 1447
PleI	GAGTC	4 cut(s)	665, 811, 1137, 1489
PpsI	GAGTC	4 cut(s)	665, 811, 1137, 1489
Psp6I	CCWGG	6 cut(s)	237, 371, 615, 1073, 1120, 1536
PspGI	CCWGG	6 cut(s)	237, 371, 615, 1073, 1120, 1536
PspN4I	GGNNCC	4 cut(s)	172, 324, 687, 1474
PspPI	GGNCC	5 cut(s)	165, 241, 584, 840, 1133
PsuI	RGATCY	4 cut(s)	50, 170, 685, 867
PvuII	CAGCTG	1 cut(s)	815
RsaI	GTAC	7 cut(s)	5, 20, 380, 443, 638, 677, 1223
RsaNI	GTAC	7 cut(s)	4, 19, 379, 442, 637, 676, 1222
SaqAI	TTAA	1 cut(s)	459
SatI	GCNGC	5 cut(s)	422, 1005, 1101, 1326, 1446
Sau3AI	GATC	8 cut(s)	50, 170, 294, 685, 760, 867, 1066, 1360
Sau96I	GGNCC	5 cut(s)	165, 241, 584, 840, 1133
ScaI	AGTACT	1 cut(s)	638
SchI	GAGTC	4 cut(s)	665, 812, 1137, 1489
SduI	GDGCHC	3 cut(s)	327, 524, 1241
SfaNI	GCATC	3 cut(s)	1072, 1413, 1432
SfcI	CTRYAG	1 cut(s)	402
Sfr274I	CTCGAG	1 cut(s)	574
SfuI	TTCGAA	1 cut(s)	1112
SinI	GGWCC	2 cut(s)	165, 241
SlaI	CTCGAG	1 cut(s)	574
SmaI	CCCGGG	1 cut(s)	189
SmlI	CTYRAG	1 cut(s)	574
SmoI	CTYRAG	1 cut(s)	574
Sse9I	AATT	3 cut(s)	846, 855, 1482
SseBI	AGGCCT	1 cut(s)	219
SsiI	CCGC	9 cut(s)	174, 286, 355, 485, 566, 1078, 1085, 1100, 1326
SspMI	CTAG	5 cut(s)	116, 965, 1169, 1413, 1430
StuI	AGGCCT	1 cut(s)	219
TaaI	ACNGT	4 cut(s)	158, 704, 1284, 1489
TaiI	ACGT	1 cut(s)	345
TaqI	TCGA	5 cut(s)	147, 222, 575, 1112, 1272
TasI	AATT	3 cut(s)	846, 855, 1482
TatI	WGTACW	3 cut(s)	3, 636, 1221
TauI	GCSGC	2 cut(s)	1103, 1328
TfiI	GAWTC	1 cut(s)	809
Tru1I	TTAA	1 cut(s)	459
Tru9I	TTAA	1 cut(s)	459
TscAI	CASTG	3 cut(s)	395, 651, 1507
TseI	GCWGC	3 cut(s)	421, 1004, 1445
TspDTI	ATGAA	4 cut(s)	167, 353, 1529, 1547
TspGWI	ACGGA	2 cut(s)	73, 842
TspMI	CCCGGG	1 cut(s)	187
TspRI	CASTG	3 cut(s)	395, 651, 1507
Van91I	CCANNNNNTGG	2 cut(s)	372, 919
VpaK11BI	GGWCC	2 cut(s)	165, 241
XapI	RAATTY	1 cut(s)	846
XbaI	TCTAGA	1 cut(s)	964
XceI	RCATGY	2 cut(s)	514, 1404
XcmI	CCANNNNNNNNNTGG	2 cut(s)	867, 1042
XhoI	CTCGAG	1 cut(s)	574
XmaI	CCCGGG	1 cut(s)	187
XmnI	GAANNNNTTC	1 cut(s)	1268
XspI	CTAG	5 cut(s)	116, 965, 1169, 1413, 1430
ZrmI	AGTACT	1 cut(s)	638
Zsp2I	ATGCAT	1 cut(s)	1406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.