MD12G1047400.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006956
Physical Location & Seq
Forward (+)
5435751 .. 5435942
192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1047400.v1.1.491

Sequence Viewer

Length: 192 bp
ATGAGCCTCAAGGAATGCAGGCGATGTTTAGAAAATTGCTCATGTAGGGCATATGCAAATCTATACATCAGGGGAAGAGGAAGCGGCTACTTGCTTTGGTTTGGTAACCTCGTTGACATAAGAGAAATCACTATTAACGAAATTCAGGACGTCTATATATGGATGGCAGCTTCAGAAATAGGTAGCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

64

Amino Acids

7.25

Weight (kDa)

6.12

Isoelectric Point (pI)

61.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAN_2 PF08276 2 - 40 6.6e-11 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
AciI CCGC 1 cut(s) 84
AcsI RAATTY 1 cut(s) 141
AcuI CTGAAG 1 cut(s) 156
AcyI GRCGYC 1 cut(s) 150
AluBI AGCT 2 cut(s) 170, 186
AluI AGCT 2 cut(s) 170, 186
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 1 cut(s) 141
BbvI GCAGC 1 cut(s) 179
BccI CCATC 1 cut(s) 157
BisI GCNGC 2 cut(s) 85, 168
BlsI GCNGC 2 cut(s) 86, 169
BsaHI GRCGYC 1 cut(s) 150
BseGI GGATG 1 cut(s) 168
BseXI GCAGC 1 cut(s) 179
BsmI GAATGC 1 cut(s) 20
BspACI CCGC 1 cut(s) 84
BssNI GRCGYC 1 cut(s) 150
Bst6I CTCTTC 1 cut(s) 70
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 20
BstEII GGTNACC 1 cut(s) 104
BstF5I GGATG 1 cut(s) 168
BstPI GGTNACC 1 cut(s) 104
BstV1I GCAGC 1 cut(s) 179
BtgZI GCGATG 1 cut(s) 37
BtsCI GGATG 1 cut(s) 168
Cac8I GCNNGC 1 cut(s) 20
CviAII CATG 1 cut(s) 42
CviJI RGCY 4 cut(s) 6, 87, 170, 186
CviKI_1 RGCY 4 cut(s) 6, 87, 170, 186
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 156
Eco91I GGTNACC 1 cut(s) 104
EcoO65I GGTNACC 1 cut(s) 104
FaeI CATG 1 cut(s) 45
FaiI YATR 9 cut(s) 43, 52, 54, 64, 119, 156, 158, 160, 190
FatI CATG 1 cut(s) 41
FauNDI CATATG 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 85, 168
FokI GGATG 1 cut(s) 175
Fsp4HI GCNGC 2 cut(s) 85, 168
GluI GCNGC 2 cut(s) 85, 168
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 45
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 1 cut(s) 115
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 2 cut(s) 18, 56
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 45
LpnPI CCDG 3 cut(s) 4, 55, 131
Lsp1109I GCAGC 1 cut(s) 179
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 104
MboII GAAGA 1 cut(s) 87
MluCI AATT 2 cut(s) 34, 141
MnlI CCTC 3 cut(s) 17, 71, 119
MseI TTAA 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 20
NdeI CATATG 1 cut(s) 52
NlaIII CATG 1 cut(s) 45
PctI GAATGC 1 cut(s) 20
PkrI GCNGC 2 cut(s) 86, 169
PspEI GGTNACC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 2 cut(s) 85, 168
SetI ASST 5 cut(s) 111, 153, 172, 184, 188
SgeI CNNG 7 cut(s) 22, 31, 54, 82, 103, 122, 158
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 2 cut(s) 34, 141
SsiI CCGC 1 cut(s) 84
TaiI ACGT 1 cut(s) 153
TasI AATT 2 cut(s) 34, 141
TauI GCSGC 1 cut(s) 87
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TseI GCWGC 1 cut(s) 167
XapI RAATTY 1 cut(s) 141
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.