MD12G1067500.v1.1

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003053
Physical Location & Seq
Forward (+)
7978266 .. 7985990
7725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1067500.v1.1.491

Sequence Viewer

Length: 1944 bp
ATGGAGGGATGGAAGACGAAGCAGAAGTCACCAGCTCTCACCCTCCAGCAGTTCATATCCACCACCGCCCCTCTCATCGACCTCGAAAAGGAAGCTGAGATATCTGCTTCCATTACAACTGGGTCCTTCAGAAACTTGGACACTGCGCAGAAGAAGGGCTCCACCATTCTCAATTTGAAATGTGTTGATGCTCAGACAGGACTCATGGGGAAGAGTCTTCTTGAATTTCAATCCACCAAAGCAGATGTTCTTCCTCCCCACAAGTTTAGCACCCATGATGTGGTTGTTCTAAAACCAAATAAGGCTGATTTGGGGGCCCCTGCTCTTGGACAAGGTGTGGTTTATCGGTTAAAGGACTCGTCAATCACTATTGCTTTTGATGATGTACCTGAAGATGGTTTAAATAGTCCTTTACGGTTAGAGAAACTAGGAAATGAGGTGACATATCGTAGGATGAAGGATGCCATGATACAATTGAGCAAAGGTGTGCAGAAGGGCCCTGCATCTGATTTAATTCCTGTCTTATTTGGAGAGAGGACACCAACAGTGTCCAAGAAGGATGTCACATTCACTCCATTTAACAAAAACCTTGATCACTCTCAGAGAGATGCCATTTCAAAGGCCCTGTCATCAAAGAATGTATTTTTGCTGCACGGACCTCCTGGCACGGGAAAGACCACAACCGTGGTGGAAATTATCTTGCAAGAAGTTAAACGTGGATCAAAGATTCTTGCATGTGCTGCTTCAAATATTGCTGTTGACAACATAGTTGAGCGACTTGCACGCCACAAAGTAAAGTTAGTGAGATTGGGGCATCCTGCACGTTTACTACCTCAAGTTCTGGACAGTGCACTAGATGCACAGGTCTTGCGAGGGGATAACAGTTCTCTGGCAAATGACATTCGGAAGGAAATGAAGGCATTAAATGGGAAGCTGTTAAGGACCAAAGATAAGAACACTAGGAGGGAAATCCAGAAGGAGCTTAGAACTCTTTCTAGGGAAGAGCGTAAAAGGCAGCAGCTGGCTGTAACAGATGTAATAAAAAATGCAGATGTAATTTTGACAACTTTGACTGGGGCATCTTCTCGCAAGCTCGACAATACTTCATTTGATTTGGTAATCATTGATGAAGCTGCTCAGGCACTTGAGATAGCATGCTGGATGGCTCTACTAAAGGGTTCAAGATGTATACTTGCAGGCGACCATCTTCAGCTTCCTCCTACCATTCAGAGTGCTGAAGCTGAGAAGAAAGGTCTAGGAAGGACCCTCTTTGAACGCCTTGCAGACATATATGGAAATGAAGTCATGTCTATGCTCACTGTCCAGTACCGCATGCACAAGCGGATCATGGATTGGTCATCAAAGGAGCTTTACAACAGTAAGATTAAGGCTCATTCAAGTGTTGCTGGGCATATGCTTTTGGATCTGGAGGATGTAAAGACGACCTCTTCTACAGAACCGACCCTTCTTCTAATAGATACAGCTGGGTGTGACATGGAAGAAAAGAAGGATGAAGAAGAAAGCACTTTGAATGAGGGTGAAGCCGATGTTGCTATTGCCCATGCCAAGAGACTTGTTCAAAGCGGTGTTCAGGCTTCTGATATTGGAATTATCACTCCTTATGCTGCACAGGTTGTCTTACTCAGGATGTTGAGAAGCAATGACGACAAGCTAAAGGATTTGGAAATCTCAACAGTTGACGGCTTCCAGGGTCGGGAAAAGGAAGCCATTATTATTTCAATGGTTCGGTCAAATTCAAAAAAAGAGGTGGGGTTTCTGAAGGACCACAGGCGAATGAATGTGGCTGTAACACGGGCAAGAAGGCAATGCTGTCTTGTATGTGACACAGAGACGGTGAGTAGTGATGCATTCTTGAAGCGTTTGATAGAGTATTTTGAGGAGCATGGCGAGTATCTAAGTGCCTCAGAGTATAGCAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000049 GO:0000122 GO:0000166 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003008 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003690 GO:0003697 GO:0003700 GO:0003723 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0007417 GO:0008026 GO:0008094 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0021510 GO:0021515 GO:0021517 GO:0021522 GO:0021953 GO:0022008 GO:0022607 GO:0030154 GO:0030182 GO:0030424 GO:0030426 GO:0030427 GO:0030554 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0032553 GO:0032555 GO:0032559 GO:0032574 GO:0032575 GO:0032991 GO:0033267 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0036477 GO:0042623 GO:0042995 GO:0043005 GO:0043021 GO:0043022 GO:0043025 GO:0043043 GO:0043139 GO:0043141 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043603 GO:0043604 GO:0043621 GO:0043933 GO:0044085 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044297 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0044877 GO:0045892 GO:0045934 GO:0048471 GO:0048513 GO:0048519 GO:0048523 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050877 GO:0050905 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051259 GO:0051260 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097367 GO:0097458 GO:0120025 GO:0120038 GO:0140097 GO:0140098 GO:0140110 GO:0150034 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:1990904 GO:1990955 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

648

Amino Acids

71.62

Weight (kDa)

8.53

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMUBP-2_HCS1_1B PF21138 21 - 130 2.7e-17 Helicase SMUBP-2/HCS1, 1B domain
AAA_11 PF13086 196 - 411 1.8e-66 AAA domain
AAA_30 PF13604 197 - 407 3.6e-09 AAA domain
AAA_19 PF13245 201 - 408 3.7e-11 AAA domain
AAA_12 PF13087 420 - 616 1.6e-62 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 280
AccI GTMKAC 1 cut(s) 1189
AciI CCGC 4 cut(s) 66, 1330, 1342, 1584
AclWI GGATC 3 cut(s) 727, 1352, 1431
AcsI RAATTY 2 cut(s) 224, 1753
AcuI CTGAAG 5 cut(s) 112, 411, 1193, 1257, 1799
AfaI GTAC 2 cut(s) 387, 1328
AfiI CCNNNNNNNGG 6 cut(s) 88, 280, 326, 395, 668, 1714
AjnI CCWGG 2 cut(s) 661, 1707
AleI CACNNNNGTG 1 cut(s) 683
Alw21I GWGCWC 1 cut(s) 853
Alw26I GTCTC 2 cut(s) 1564, 1844
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 3 cut(s) 727, 1352, 1431
AlwNI CAGNNNCTG 1 cut(s) 1021
AoxI GGCC 3 cut(s) 315, 496, 621
ApaI GGGCCC 2 cut(s) 319, 500
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 6 cut(s) 649, 740, 1015, 1018, 1133, 1625
ApoI RAATTY 2 cut(s) 224, 1753
ArsI GACNNNNNNTTYG 4 cut(s) 11, 43, 611, 643
Asp700I GAANNNNTTC 1 cut(s) 991
AspLEI GCGC 1 cut(s) 148
AsuHPI GGTGA 5 cut(s) 21, 31, 451, 1550, 1867
AvaII GGWCC 5 cut(s) 123, 656, 942, 1263, 1783
BaeGI GKGCMC 3 cut(s) 319, 500, 853
BanII GRGCYC 3 cut(s) 161, 319, 500
BbsI GAAGAC 2 cut(s) 20, 209
Bbv12I GWGCWC 1 cut(s) 853
BbvI GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1612
BccI CCATC 4 cut(s) 3, 389, 1156, 1212
BceAI ACGGC 1 cut(s) 1717
BciT130I CCWGG 2 cut(s) 663, 1709
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 2 cut(s) 1564, 1844
BfaI CTAG 5 cut(s) 428, 854, 960, 996, 1256
BfmI CTRYAG 1 cut(s) 1452
BisI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
BlsI GCNGC 6 cut(s) 651, 742, 1017, 1020, 1135, 1627
Bme1390I CCNGG 2 cut(s) 663, 1709
Bme18I GGWCC 5 cut(s) 123, 656, 942, 1263, 1783
BmiI GGNNCC 7 cut(s) 124, 160, 316, 317, 318, 498, 1265
BmrFI CCNGG 2 cut(s) 663, 1709
BmrI ACTGGG 2 cut(s) 129, 1083
BmsI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1855
BmuI ACTGGG 2 cut(s) 129, 1083
BpiI GAAGAC 2 cut(s) 20, 209
BpmI CTGGAG 2 cut(s) 29, 1448
Bpu10I CCTNAGC 1 cut(s) 1137
BpuEI CTTGAG 2 cut(s) 819, 1166
BsaJI CCNNGG 2 cut(s) 684, 1708
BsaXI ACNNNNNCTCC 2 cut(s) 556, 586
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 280, 326, 395, 668, 1714
Bse1I ACTGG 3 cut(s) 124, 1078, 1324
Bse3DI GCAATG 3 cut(s) 1666, 1832, 1942
BseBI CCWGG 2 cut(s) 663, 1709
BseDI CCNNGG 2 cut(s) 684, 1708
BseGI GGATG 9 cut(s) 14, 459, 466, 565, 814, 1167, 1438, 1516, 1653
BseLI CCNNNNNNNGG 6 cut(s) 88, 280, 326, 395, 668, 1714
BseMI GCAATG 3 cut(s) 1666, 1832, 1942
BseMII CTCAG 7 cut(s) 87, 206, 614, 1151, 1233, 1657, 1938
BseNI ACTGG 3 cut(s) 124, 1078, 1324
BseRI GAGGAG 1 cut(s) 1913
BseSI GKGCMC 3 cut(s) 319, 500, 853
BseXI GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1612
BseYI CCCAGC 2 cut(s) 1406, 1484
BsgI GTGCAG 4 cut(s) 509, 635, 804, 1611
BshFI GGCC 3 cut(s) 317, 498, 623
BsiHKAI GWGCWC 1 cut(s) 853
BslI CCNNNNNNNGG 6 cut(s) 88, 280, 326, 395, 668, 1714
BsmAI GTCTC 2 cut(s) 1564, 1844
BsmBI CGTCTC 1 cut(s) 1844
BsmI GAATGC 1 cut(s) 1868
BsnI GGCC 3 cut(s) 317, 498, 623
Bsp120I GGGCCC 2 cut(s) 315, 496
Bsp1286I GDGCHC 4 cut(s) 161, 319, 500, 853
Bsp143I GATC 4 cut(s) 592, 719, 1344, 1423
BspACI CCGC 4 cut(s) 66, 1330, 1342, 1584
BspANI GGCC 3 cut(s) 317, 498, 623
BspCNI CTCAG 7 cut(s) 88, 205, 613, 1150, 1234, 1656, 1937
BspLI GGNNCC 7 cut(s) 124, 160, 316, 317, 318, 498, 1265
BspPI GGATC 3 cut(s) 727, 1352, 1431
BspQI GCTCTTC 1 cut(s) 996
BsrDI GCAATG 3 cut(s) 1666, 1832, 1942
BsrI ACTGG 3 cut(s) 124, 1078, 1324
BssECI CCNNGG 2 cut(s) 684, 1708
BssMI GATC 4 cut(s) 592, 719, 1344, 1423
BssNAI GTATAC 1 cut(s) 1190
Bst1107I GTATAC 1 cut(s) 1190
Bst2UI CCWGG 2 cut(s) 663, 1709
Bst4CI ACNGT 9 cut(s) 417, 547, 685, 848, 884, 1321, 1379, 1696, 1855
Bst6I CTCTTC 3 cut(s) 206, 996, 1453
BstAPI GCANNNNNTGC 2 cut(s) 740, 857
BstC8I GCNNGC 6 cut(s) 784, 1023, 1091, 1156, 1198, 1334
BstDEI CTNAG 9 cut(s) 96, 192, 600, 983, 1137, 1242, 1643, 1916, 1924
BstDSI CCRYGG 1 cut(s) 684
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 9 cut(s) 14, 459, 466, 565, 814, 1167, 1438, 1516, 1653
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 4 cut(s) 595, 722, 1347, 1426
BstMAI GTCTC 2 cut(s) 1564, 1844
BstMBI GATC 4 cut(s) 592, 719, 1344, 1423
BstMWI GCNNNNNNNGC 5 cut(s) 740, 857, 1012, 1139, 1550
BstNI CCWGG 2 cut(s) 663, 1709
BstNSI RCATGY 3 cut(s) 738, 1158, 1336
BstSCI CCNGG 2 cut(s) 661, 1707
BstSFI CTRYAG 1 cut(s) 1452
BstSLI GKGCMC 3 cut(s) 319, 500, 853
BstV1I GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1612
BstV2I GAAGAC 2 cut(s) 20, 209
BstX2I RGATCY 1 cut(s) 1423
BstXI CCANNNNNNTGG 1 cut(s) 685
BstYI RGATCY 1 cut(s) 1423
BstZ17I GTATAC 1 cut(s) 1190
BsuRI GGCC 3 cut(s) 317, 498, 623
BtgI CCRYGG 1 cut(s) 684
BtsCI GGATG 9 cut(s) 14, 459, 466, 565, 814, 1167, 1438, 1516, 1653
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 4 cut(s) 141, 552, 853, 1317
Cac8I GCNNGC 6 cut(s) 784, 1023, 1091, 1156, 1198, 1334
CaiI CAGNNNCTG 1 cut(s) 1021
CfoI GCGC 1 cut(s) 148
Csp6I GTAC 2 cut(s) 386, 1327
CspCI CAANNNNNGTGG 2 cut(s) 669, 704
CviQI GTAC 2 cut(s) 386, 1327
DdeI CTNAG 9 cut(s) 96, 192, 600, 983, 1137, 1242, 1643, 1916, 1924
DpnI GATC 4 cut(s) 594, 721, 1346, 1425
DpnII GATC 4 cut(s) 592, 719, 1344, 1423
DraI TTTAAA 1 cut(s) 402
Eam1104I CTCTTC 3 cut(s) 206, 996, 1453
EarI CTCTTC 3 cut(s) 206, 996, 1453
Eco24I GRGCYC 3 cut(s) 161, 319, 500
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 5 cut(s) 123, 656, 942, 1263, 1783
Eco57I CTGAAG 5 cut(s) 112, 411, 1193, 1257, 1799
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoO109I RGGNCCY 7 cut(s) 123, 315, 316, 496, 497, 622, 1263
EcoRII CCWGG 2 cut(s) 661, 1707
EcoRV GATATC 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 1870
EcoT38I GRGCYC 3 cut(s) 161, 319, 500
Esp3I CGTCTC 1 cut(s) 1844
FauNDI CATATG 1 cut(s) 1413
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 1189
Fnu4HI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
FokI GGATG 9 cut(s) 21, 466, 473, 572, 801, 1174, 1445, 1523, 1660
FriOI GRGCYC 3 cut(s) 161, 319, 500
Fsp4HI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
FspBI CTAG 5 cut(s) 428, 854, 960, 996, 1256
FspI TGCGCA 1 cut(s) 147
GlaI GCGC 1 cut(s) 147
GluI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
GsaI CCCAGC 2 cut(s) 1410, 1488
GsuI CTGGAG 2 cut(s) 29, 1448
HaeIII GGCC 3 cut(s) 317, 498, 623
HhaI GCGC 1 cut(s) 148
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 2 cut(s) 760, 1699
HindII GTYRAC 2 cut(s) 760, 1699
HinfI GANTC 4 cut(s) 201, 214, 356, 727
HphI GGTGA 5 cut(s) 21, 31, 451, 1550, 1867
Hpy166II GTNNAC 5 cut(s) 760, 827, 851, 1190, 1699
Hpy188I TCNGA 9 cut(s) 131, 195, 508, 603, 906, 1230, 1600, 1779, 1927
Hpy188III TCNNGA 8 cut(s) 221, 842, 973, 1182, 1427, 1645, 1715, 1873
Hpy8I GTNNAC 5 cut(s) 760, 827, 851, 1190, 1699
HpyCH4III ACNGT 9 cut(s) 417, 547, 685, 848, 884, 1321, 1379, 1696, 1855
HpyCH4IV ACGT 2 cut(s) 715, 823
HpyF10VI GCNNNNNNNGC 5 cut(s) 740, 857, 1012, 1139, 1550
HpyF3I CTNAG 9 cut(s) 96, 192, 600, 983, 1137, 1242, 1643, 1916, 1924
HpySE526I ACGT 2 cut(s) 715, 823
HspAI GCGC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 4 cut(s) 592, 719, 1344, 1423
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 4 cut(s) 164, 979, 1366, 1900
Lsp1109I GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1612
LweI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1855
MaeI CTAG 5 cut(s) 428, 854, 960, 996, 1256
MaeII ACGT 2 cut(s) 715, 823
MaeIII GTNAC 7 cut(s) 27, 439, 562, 1027, 1490, 1807, 1841
MalI GATC 4 cut(s) 594, 721, 1346, 1425
MboI GATC 4 cut(s) 592, 719, 1344, 1423
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 1 cut(s) 1423
MhlI GDGCHC 4 cut(s) 161, 319, 500, 853
MluCI AATT 8 cut(s) 172, 224, 473, 513, 693, 1056, 1608, 1753
MlyI GAGTC 3 cut(s) 195, 223, 350
Mph1103I ATGCAT 1 cut(s) 1870
MroXI GAANNNNTTC 1 cut(s) 991
MseI TTAA 8 cut(s) 350, 401, 512, 579, 711, 923, 938, 1386
MslI CAYNNNNRTG 3 cut(s) 683, 1310, 1398
MspA1I CMGCKG 2 cut(s) 1021, 1484
MspR9I CCNGG 2 cut(s) 663, 1709
MunI CAATTG 1 cut(s) 473
Mva1269I GAATGC 1 cut(s) 1868
MvaI CCWGG 2 cut(s) 663, 1709
MwoI GCNNNNNNNGC 5 cut(s) 740, 857, 1012, 1139, 1550
NdeI CATATG 1 cut(s) 1413
NdeII GATC 4 cut(s) 592, 719, 1344, 1423
NlaIV GGNNCC 7 cut(s) 124, 160, 316, 317, 318, 498, 1265
NmuCI GTSAC 5 cut(s) 27, 439, 562, 1490, 1841
NsbI TGCGCA 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 1870
NspI RCATGY 3 cut(s) 738, 1158, 1336
OliI CACNNNNGTG 1 cut(s) 683
PaeI GCATGC 2 cut(s) 1158, 1336
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 1 cut(s) 1868
PdmI GAANNNNTTC 1 cut(s) 991
PfeI GAWTC 1 cut(s) 727
PflMI CCANNNNNTGG 1 cut(s) 280
PkrI GCNGC 6 cut(s) 651, 742, 1017, 1020, 1135, 1627
PleI GAGTC 3 cut(s) 195, 222, 350
PpsI GAGTC 3 cut(s) 195, 222, 350
PpuMI RGGWCCY 2 cut(s) 123, 1263
Psp5II RGGWCCY 2 cut(s) 123, 1263
Psp6I CCWGG 2 cut(s) 661, 1707
PspFI CCCAGC 2 cut(s) 1406, 1484
PspGI CCWGG 2 cut(s) 661, 1707
PspN4I GGNNCC 7 cut(s) 124, 160, 316, 317, 318, 498, 1265
PspOMI GGGCCC 2 cut(s) 315, 496
PspPPI RGGWCCY 2 cut(s) 123, 1263
PstNI CAGNNNCTG 1 cut(s) 1021
PsuI RGATCY 1 cut(s) 1423
PvuII CAGCTG 2 cut(s) 1021, 1484
RsaI GTAC 2 cut(s) 387, 1328
RsaNI GTAC 2 cut(s) 386, 1327
RseI CAYNNNNRTG 3 cut(s) 683, 1310, 1398
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 8 cut(s) 350, 401, 512, 579, 711, 923, 938, 1386
SatI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
Sau3AI GATC 4 cut(s) 592, 719, 1344, 1423
SchI GAGTC 3 cut(s) 195, 223, 350
ScrFI CCNGG 2 cut(s) 663, 1709
SduI GDGCHC 4 cut(s) 161, 319, 500, 853
SfaNI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1855
SfcI CTRYAG 1 cut(s) 1452
SinI GGWCC 5 cut(s) 123, 656, 942, 1263, 1783
SmiMI CAYNNNNRTG 3 cut(s) 683, 1310, 1398
SmlI CTYRAG 2 cut(s) 834, 1145
SmoI CTYRAG 2 cut(s) 834, 1145
SphI GCATGC 2 cut(s) 1158, 1336
Sse9I AATT 8 cut(s) 172, 224, 473, 513, 693, 1056, 1608, 1753
SsiI CCGC 4 cut(s) 66, 1330, 1342, 1584
SspI AATATT 1 cut(s) 751
SspMI CTAG 5 cut(s) 428, 854, 960, 996, 1256
StyD4I CCNGG 2 cut(s) 661, 1707
TaaI ACNGT 9 cut(s) 417, 547, 685, 848, 884, 1321, 1379, 1696, 1855
TaiI ACGT 2 cut(s) 718, 826
TaqI TCGA 3 cut(s) 78, 84, 1095
TaqII GACCGA 1 cut(s) 1737
TasI AATT 8 cut(s) 172, 224, 473, 513, 693, 1056, 1608, 1753
TfiI GAWTC 1 cut(s) 727
Tru1I TTAA 8 cut(s) 350, 401, 512, 579, 711, 923, 938, 1386
Tru9I TTAA 8 cut(s) 350, 401, 512, 579, 711, 923, 938, 1386
TscAI CASTG 4 cut(s) 148, 552, 853, 1324
TseFI GTSAC 5 cut(s) 27, 439, 562, 1490, 1841
TseI GCWGC 6 cut(s) 649, 740, 1015, 1018, 1133, 1625
Tsp45I GTSAC 5 cut(s) 27, 439, 562, 1490, 1841
TspDTI ATGAA 8 cut(s) 43, 470, 929, 1095, 1143, 1314, 1527, 1811
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 4 cut(s) 148, 552, 853, 1324
Van91I CCANNNNNTGG 1 cut(s) 280
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 5 cut(s) 123, 656, 942, 1263, 1783
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 2 cut(s) 224, 1753
XceI RCATGY 3 cut(s) 738, 1158, 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmiI GTMKAC 1 cut(s) 1189
XmnI GAANNNNTTC 1 cut(s) 991
XspI CTAG 5 cut(s) 428, 854, 960, 996, 1256
Zsp2I ATGCAT 1 cut(s) 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.