MD14G1067600.v1.1

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003053
Physical Location & Seq
Forward (+)
7362240 .. 7370401
8162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1067600.v1.1.491

Sequence Viewer

Length: 1944 bp
ATGGAGGGAGGGAAGACGAAGCAGAAATCGCCGGCTCTGACTCTCCAACAGTTCATCTCCACCACCGCCCCTCTTATCGACCTCGAAAAGGAAGCTGAGATATCTGCCTCCATCATAACTGGGTCGTCCAGAAACTTGGACACTGCTCAGAAGAAGGGCTCCACCATTCTCAATTTGAAATGTGTTGATGCTCAGACTGGACTCATGGGGAAGAGTCTTCTTGAGTTTCAATCCACCAAAGCAGATGTTCTTCCTCCCCACAAGTTTAGCACCCATGATGTGGTTGTTCTAAAACCAAATAAGGCTGATCTGGGGGCCTCTGCTCTTGGACAAGGTGTAGTTTATCGGTTAAAGGACTCATCAATCATTGTTGCTTTTGATGATGTTCCTGAAGATGGTTTAAATAGTCCTCTACGGTTAGAGAAACTGGGAAATGAGGTGACATATCGTAGGATGAAGGATGCCTTGATACAATTGAGCAAAGGTGTGCAGAAGGGCCCCGCATCTGATTTAATTCCTGTCTTATTTGGAGAGAGGACACCAACAGTGTCCAAGAAGGATGTCACATTTACCCCATTTAACAAAAACCTTGATCATTCTCAGAGAGATGCCATTTCAAAGGCCCTGTCATCAAAGAATGTATTTTTGCTGCATGGACCTCCTGGCACGGGAAAGACTACAACTGTGGTGGAAATTATCTTGCAAGAAGTTAAACGTGGATCAAAGATTCTTGCATGTGCTGCTTCAAATATTGCTGTTGACAACATTGTTGAGCGACTTGCACGCCACAAAGTAAAGTTAGTGAGATTGGGGCATCCTGCACGTTTACTACCTCAAGTACTGGACAGTGCACTGGATGCACAGGTCTTGCGAGGGGATAACAGTTCACTGGCAAATGACATTCGGAAGGAAATGAAGGCATTAAATGGGAAGCTGTTAAGGACCAAAGATAAGAACACTAGGAGGGAAATCCAGAAGGAGCTTAGGACTCTTTCTAGGGAAGAGCGTAAAAGGCAGCAGCTAGCTGTAACAGATGTAATAAAAAATGCAGATGTGATTTTGGCAACTTTGACTGGGGCATCTTCTCGCAAGCTTGACAATACTTCATTTGATTTGGTGATTATTGATGAAGCTGCTCAGGCACTTGAGATAGCATGCTGGATGGCTTTACTAAAGGGTTCAAGATGTATACTTGCAGGCGACCATCTTCAGCTTCCTCCTACCATTCAGAGTGCTGAAGCTGAGAAGAAGGGTCTAGGAAGGACCCTCTTTGAACGCCTTGCAGACATATATGGAAATGAAGTCATGTCTATGCTCACCGTCCAGTACCGCATGCACGAGCGGATCATGGATTGGTCATCAAAGGAGCTTTACAACAGTAAGATTAAGGCCCATTCAAGTGTTGCTGGGCATATGCTTTTGGACCTGGAGGATGTAAAGACGACCTCTTCTACAGAACCGACCCTTCTTCTAATAGACACAGCTGGGTGTGACATGGAAGAAAAGAAGGATGAAGAAGAAAGCACTTTGAATGAGGGGGAAGCCGATGTTGCTATTGCCCATGCCAAGAGGCTTGTTCAAAGTGGTGTTCAGGCTTCTGATATTGGAATTATCACTCCTTATGCTGCACAGGTTGTCTTACTCAGGATGTTGAGAAGCAATGACGACAAGCTAAAGGATTTGGAAATCTCAACAGTTGACGGCTTCCAGGGCCGGGAAAAGGAAGCCATCATTATTTCAATGGTTCGGTCAAATTCAAAAAAAGAGGTAGGGTTTCTGAAGGACCACAGGCGAATGAACGTGGCTGTAACACGTGCAAGAAGGCAATGCTGTCTTGTATGCGACACAGACACGGTGACTAGTGATGCATTCCTGAAGCGATTGATAGAGTATTTTGAGGAGCATGGTGAGTATCTAAGTGCCTCAGAGTATAGCAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000049 GO:0000122 GO:0000166 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003008 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003690 GO:0003697 GO:0003700 GO:0003723 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0007417 GO:0008026 GO:0008094 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0021510 GO:0021515 GO:0021517 GO:0021522 GO:0021953 GO:0022008 GO:0022607 GO:0030154 GO:0030182 GO:0030424 GO:0030426 GO:0030427 GO:0030554 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0032553 GO:0032555 GO:0032559 GO:0032574 GO:0032575 GO:0032991 GO:0033267 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0036477 GO:0042623 GO:0042995 GO:0043005 GO:0043021 GO:0043022 GO:0043025 GO:0043043 GO:0043139 GO:0043141 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043603 GO:0043604 GO:0043621 GO:0043933 GO:0044085 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044297 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0044877 GO:0045892 GO:0045934 GO:0048471 GO:0048513 GO:0048519 GO:0048523 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050877 GO:0050905 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051259 GO:0051260 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097367 GO:0097458 GO:0120025 GO:0120038 GO:0140097 GO:0140098 GO:0140110 GO:0150034 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:1990904 GO:1990955 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

648

Amino Acids

71.38

Weight (kDa)

8.22

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMUBP-2_HCS1_1B PF21138 21 - 130 8.2e-18 Helicase SMUBP-2/HCS1, 1B domain
AAA_11 PF13086 196 - 411 2.5e-66 AAA domain
AAA_30 PF13604 197 - 407 3.8e-09 AAA domain
AAA_19 PF13245 201 - 408 3.5e-11 AAA domain
AAA_12 PF13087 420 - 616 1.2e-62 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 280
AccBSI CCGCTC 1 cut(s) 1342
AccI GTMKAC 1 cut(s) 1189
AciI CCGC 4 cut(s) 66, 501, 1330, 1342
AclWI GGATC 2 cut(s) 727, 1352
AcsI RAATTY 1 cut(s) 1753
AcuI CTGAAG 5 cut(s) 411, 1193, 1257, 1799, 1895
AcvI CACGTG 1 cut(s) 1814
AfaI GTAC 2 cut(s) 840, 1328
AfiI CCNNNNNNNGG 6 cut(s) 88, 280, 395, 668, 1714, 1720
AflIII ACRYGT 1 cut(s) 1811
AhlI ACTAGT 1 cut(s) 1859
AjnI CCWGG 3 cut(s) 661, 1425, 1707
Alw21I GWGCWC 1 cut(s) 853
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 2 cut(s) 727, 1352
AoxI GGCC 5 cut(s) 315, 496, 621, 1389, 1711
ApaI GGGCCC 1 cut(s) 500
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 6 cut(s) 649, 740, 1015, 1018, 1133, 1625
ApoI RAATTY 1 cut(s) 1753
ArsI GACNNNNNNTTYG 2 cut(s) 611, 643
AsuC2I CCSGG 1 cut(s) 1715
AsuHPI GGTGA 5 cut(s) 451, 1129, 1309, 1867, 1919
AsuNHI GCTAGC 1 cut(s) 1021
AvaII GGWCC 5 cut(s) 656, 942, 1263, 1423, 1783
BaeGI GKGCMC 2 cut(s) 500, 853
BanII GRGCYC 2 cut(s) 161, 500
BauI CACGAG 1 cut(s) 1337
BbrPI CACGTG 1 cut(s) 1814
BbsI GAAGAC 2 cut(s) 20, 209
Bbv12I GWGCWC 1 cut(s) 853
BbvI GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1612
BccI CCATC 5 cut(s) 119, 389, 1156, 1212, 1736
BceAI ACGGC 1 cut(s) 1717
BciT130I CCWGG 3 cut(s) 663, 1427, 1709
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 1 cut(s) 1715
BcuI ACTAGT 1 cut(s) 1859
BfaI CTAG 5 cut(s) 960, 996, 1022, 1256, 1860
BfmI CTRYAG 1 cut(s) 1452
BisI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
BlsI GCNGC 6 cut(s) 651, 742, 1017, 1020, 1135, 1627
BmcAI AGTACT 1 cut(s) 840
Bme1390I CCNGG 4 cut(s) 663, 1427, 1709, 1715
Bme18I GGWCC 5 cut(s) 656, 942, 1263, 1423, 1783
BmiI GGNNCC 5 cut(s) 160, 316, 498, 499, 1265
BmrFI CCNGG 4 cut(s) 663, 1427, 1709, 1715
BmrI ACTGGG 3 cut(s) 129, 437, 1083
BmsI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1855
BmtI GCTAGC 1 cut(s) 1025
BmuI ACTGGG 3 cut(s) 129, 437, 1083
BpiI GAAGAC 2 cut(s) 20, 209
BpmI CTGGAG 1 cut(s) 1448
Bpu10I CCTNAGC 2 cut(s) 983, 1137
BpuEI CTTGAG 3 cut(s) 242, 819, 1166
BpuMI CCSGG 1 cut(s) 1715
BsaAI YACGTR 1 cut(s) 1814
BsaJI CCNNGG 1 cut(s) 1708
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 280, 395, 668, 1714, 1720
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 8 cut(s) 124, 202, 432, 846, 858, 894, 1078, 1324
Bse3DI GCAATG 3 cut(s) 1666, 1832, 1942
BseBI CCWGG 3 cut(s) 663, 1427, 1709
BseDI CCNNGG 1 cut(s) 1708
BseGI GGATG 9 cut(s) 459, 466, 565, 814, 862, 1167, 1438, 1516, 1653
BseLI CCNNNNNNNGG 6 cut(s) 88, 280, 395, 668, 1714, 1720
BseMI GCAATG 3 cut(s) 1666, 1832, 1942
BseMII CTCAG 8 cut(s) 87, 161, 206, 614, 1151, 1233, 1657, 1938
BseNI ACTGG 8 cut(s) 124, 202, 432, 846, 858, 894, 1078, 1324
BseRI GAGGAG 1 cut(s) 1913
BseSI GKGCMC 2 cut(s) 500, 853
BseXI GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1612
BseYI CCCAGC 2 cut(s) 1406, 1484
BsgI GTGCAG 3 cut(s) 509, 804, 1611
BshFI GGCC 5 cut(s) 317, 498, 623, 1391, 1713
BsiHKAI GWGCWC 1 cut(s) 853
BsiSI CCGG 2 cut(s) 32, 1714
BslI CCNNNNNNNGG 6 cut(s) 88, 280, 395, 668, 1714, 1720
BsmI GAATGC 1 cut(s) 1868
BsnI GGCC 5 cut(s) 317, 498, 623, 1391, 1713
Bsp120I GGGCCC 1 cut(s) 496
Bsp1286I GDGCHC 3 cut(s) 161, 500, 853
Bsp143I GATC 4 cut(s) 307, 592, 719, 1344
BspACI CCGC 4 cut(s) 66, 501, 1330, 1342
BspANI GGCC 5 cut(s) 317, 498, 623, 1391, 1713
BspCNI CTCAG 8 cut(s) 88, 160, 205, 613, 1150, 1234, 1656, 1937
BspLI GGNNCC 5 cut(s) 160, 316, 498, 499, 1265
BspOI GCTAGC 1 cut(s) 1025
BspPI GGATC 2 cut(s) 727, 1352
BspQI GCTCTTC 1 cut(s) 996
BsrBI CCGCTC 1 cut(s) 1342
BsrDI GCAATG 3 cut(s) 1666, 1832, 1942
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 8 cut(s) 124, 202, 432, 846, 858, 894, 1078, 1324
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 1708
BssMI GATC 4 cut(s) 307, 592, 719, 1344
BssNAI GTATAC 1 cut(s) 1190
BssSI CACGAG 1 cut(s) 1337
Bst1107I GTATAC 1 cut(s) 1190
Bst2BI CACGAG 1 cut(s) 1337
Bst2UI CCWGG 3 cut(s) 663, 1427, 1709
Bst6I CTCTTC 3 cut(s) 206, 996, 1453
BstAPI GCANNNNNTGC 2 cut(s) 740, 857
BstBAI YACGTR 1 cut(s) 1814
BstC8I GCNNGC 7 cut(s) 33, 784, 1023, 1091, 1156, 1198, 1334
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 9 cut(s) 459, 466, 565, 814, 862, 1167, 1438, 1516, 1653
BstKTI GATC 4 cut(s) 310, 595, 722, 1347
BstMBI GATC 4 cut(s) 307, 592, 719, 1344
BstMWI GCNNNNNNNGC 7 cut(s) 28, 740, 857, 1012, 1139, 1550, 1710
BstNI CCWGG 3 cut(s) 663, 1427, 1709
BstNSI RCATGY 3 cut(s) 738, 1158, 1336
BstSCI CCNGG 4 cut(s) 661, 1425, 1707, 1713
BstSFI CTRYAG 1 cut(s) 1452
BstSLI GKGCMC 2 cut(s) 500, 853
BstV1I GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1612
BstV2I GAAGAC 2 cut(s) 20, 209
BstXI CCANNNNNNTGG 1 cut(s) 136
BstZ17I GTATAC 1 cut(s) 1190
BsuRI GGCC 5 cut(s) 317, 498, 623, 1391, 1713
BtsCI GGATG 9 cut(s) 459, 466, 565, 814, 862, 1167, 1438, 1516, 1653
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 5 cut(s) 141, 552, 851, 853, 887
Cac8I GCNNGC 7 cut(s) 33, 784, 1023, 1091, 1156, 1198, 1334
Cfr10I RCCGGY 1 cut(s) 31
Csp6I GTAC 2 cut(s) 839, 1327
CspCI CAANNNNNGTGG 2 cut(s) 669, 704
CviQI GTAC 2 cut(s) 839, 1327
DpnI GATC 4 cut(s) 309, 594, 721, 1346
DpnII GATC 4 cut(s) 307, 592, 719, 1344
DraI TTTAAA 1 cut(s) 402
Eam1104I CTCTTC 3 cut(s) 206, 996, 1453
EarI CTCTTC 3 cut(s) 206, 996, 1453
Eco24I GRGCYC 2 cut(s) 161, 500
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 5 cut(s) 656, 942, 1263, 1423, 1783
Eco57I CTGAAG 5 cut(s) 411, 1193, 1257, 1799, 1895
Eco72I CACGTG 1 cut(s) 1814
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoO109I RGGNCCY 5 cut(s) 315, 496, 497, 622, 1263
EcoRII CCWGG 3 cut(s) 661, 1425, 1707
EcoRV GATATC 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 1870
EcoT38I GRGCYC 2 cut(s) 161, 500
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FauI CCCGC 1 cut(s) 508
FauNDI CATATG 1 cut(s) 1413
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 1189
Fnu4HI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
FokI GGATG 9 cut(s) 466, 473, 572, 801, 869, 1174, 1445, 1523, 1660
FriOI GRGCYC 2 cut(s) 161, 500
Fsp4HI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
FspBI CTAG 5 cut(s) 960, 996, 1022, 1256, 1860
GluI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
GsaI CCCAGC 2 cut(s) 1410, 1488
GsuI CTGGAG 1 cut(s) 1448
HaeIII GGCC 5 cut(s) 317, 498, 623, 1391, 1713
HapII CCGG 2 cut(s) 32, 1714
HincII GTYRAC 2 cut(s) 760, 1699
HindII GTYRAC 2 cut(s) 760, 1699
HindIII AAGCTT 1 cut(s) 1091
HinfI GANTC 6 cut(s) 40, 201, 214, 356, 727, 988
HpaII CCGG 2 cut(s) 32, 1714
HphI GGTGA 5 cut(s) 451, 1129, 1309, 1867, 1919
Hpy166II GTNNAC 6 cut(s) 760, 827, 851, 887, 1190, 1699
Hpy188III TCNNGA 7 cut(s) 129, 221, 389, 973, 1182, 1645, 1873
Hpy8I GTNNAC 6 cut(s) 760, 827, 851, 887, 1190, 1699
HpyCH4IV ACGT 4 cut(s) 715, 823, 1800, 1813
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 740, 857, 1012, 1139, 1550, 1710
HpySE526I ACGT 4 cut(s) 715, 823, 1800, 1813
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 4 cut(s) 307, 592, 719, 1344
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 4 cut(s) 164, 979, 1366, 1900
Lsp1109I GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1612
LweI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1855
MaeI CTAG 5 cut(s) 960, 996, 1022, 1256, 1860
MaeII ACGT 4 cut(s) 715, 823, 1800, 1813
MaeIII GTNAC 6 cut(s) 439, 562, 1027, 1490, 1807, 1855
MalI GATC 4 cut(s) 309, 594, 721, 1346
MbiI CCGCTC 1 cut(s) 1342
MboI GATC 4 cut(s) 307, 592, 719, 1344
MfeI CAATTG 1 cut(s) 473
MhlI GDGCHC 3 cut(s) 161, 500, 853
MluCI AATT 6 cut(s) 172, 473, 513, 693, 1608, 1753
MlyI GAGTC 5 cut(s) 34, 195, 223, 350, 982
MmeI TCCRAC 1 cut(s) 70
Mph1103I ATGCAT 1 cut(s) 1870
MroNI GCCGGC 1 cut(s) 31
MseI TTAA 8 cut(s) 350, 401, 512, 579, 711, 923, 938, 1386
MslI CAYNNNNRTG 2 cut(s) 1310, 1398
MspA1I CMGCKG 1 cut(s) 1484
MspI CCGG 2 cut(s) 32, 1714
MspR9I CCNGG 4 cut(s) 663, 1427, 1709, 1715
MunI CAATTG 1 cut(s) 473
Mva1269I GAATGC 1 cut(s) 1868
MvaI CCWGG 3 cut(s) 663, 1427, 1709
MwoI GCNNNNNNNGC 7 cut(s) 28, 740, 857, 1012, 1139, 1550, 1710
NaeI GCCGGC 1 cut(s) 33
NciI CCSGG 1 cut(s) 1715
NdeI CATATG 1 cut(s) 1413
NdeII GATC 4 cut(s) 307, 592, 719, 1344
NgoMIV GCCGGC 1 cut(s) 31
NheI GCTAGC 1 cut(s) 1021
NlaIV GGNNCC 5 cut(s) 160, 316, 498, 499, 1265
NmuCI GTSAC 4 cut(s) 439, 562, 1490, 1855
NsiI ATGCAT 1 cut(s) 1870
NspI RCATGY 3 cut(s) 738, 1158, 1336
PaeI GCATGC 2 cut(s) 1158, 1336
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 1 cut(s) 1868
PdiI GCCGGC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 727
PflMI CCANNNNNTGG 1 cut(s) 280
PkrI GCNGC 6 cut(s) 651, 742, 1017, 1020, 1135, 1627
PleI GAGTC 5 cut(s) 34, 195, 222, 350, 982
PmaCI CACGTG 1 cut(s) 1814
PmlI CACGTG 1 cut(s) 1814
PpsI GAGTC 5 cut(s) 34, 195, 222, 350, 982
Ppu21I YACGTR 1 cut(s) 1814
PpuMI RGGWCCY 1 cut(s) 1263
Psp5II RGGWCCY 1 cut(s) 1263
Psp6I CCWGG 3 cut(s) 661, 1425, 1707
PspCI CACGTG 1 cut(s) 1814
PspFI CCCAGC 2 cut(s) 1406, 1484
PspGI CCWGG 3 cut(s) 661, 1425, 1707
PspN4I GGNNCC 5 cut(s) 160, 316, 498, 499, 1265
PspOMI GGGCCC 1 cut(s) 496
PspPPI RGGWCCY 1 cut(s) 1263
PvuII CAGCTG 1 cut(s) 1484
RsaI GTAC 2 cut(s) 840, 1328
RsaNI GTAC 2 cut(s) 839, 1327
RseI CAYNNNNRTG 2 cut(s) 1310, 1398
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 8 cut(s) 350, 401, 512, 579, 711, 923, 938, 1386
SatI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1626
Sau3AI GATC 4 cut(s) 307, 592, 719, 1344
ScaI AGTACT 1 cut(s) 840
SchI GAGTC 5 cut(s) 34, 195, 223, 350, 982
ScrFI CCNGG 4 cut(s) 663, 1427, 1709, 1715
SduI GDGCHC 3 cut(s) 161, 500, 853
SfaNI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1855
SfcI CTRYAG 1 cut(s) 1452
SinI GGWCC 5 cut(s) 656, 942, 1263, 1423, 1783
SmiMI CAYNNNNRTG 2 cut(s) 1310, 1398
SmlI CTYRAG 3 cut(s) 221, 834, 1145
SmoI CTYRAG 3 cut(s) 221, 834, 1145
SpeI ACTAGT 1 cut(s) 1859
SphI GCATGC 2 cut(s) 1158, 1336
Sse9I AATT 6 cut(s) 172, 473, 513, 693, 1608, 1753
SsiI CCGC 4 cut(s) 66, 501, 1330, 1342
SspI AATATT 1 cut(s) 751
SspMI CTAG 5 cut(s) 960, 996, 1022, 1256, 1860
StyD4I CCNGG 4 cut(s) 661, 1425, 1707, 1713
TaiI ACGT 4 cut(s) 718, 826, 1803, 1816
TaqI TCGA 2 cut(s) 78, 84
TaqII GACCGA 1 cut(s) 1737
TasI AATT 6 cut(s) 172, 473, 513, 693, 1608, 1753
TatI WGTACW 1 cut(s) 838
TfiI GAWTC 1 cut(s) 727
Tru1I TTAA 8 cut(s) 350, 401, 512, 579, 711, 923, 938, 1386
Tru9I TTAA 8 cut(s) 350, 401, 512, 579, 711, 923, 938, 1386
TscAI CASTG 5 cut(s) 148, 552, 853, 858, 894
TseFI GTSAC 4 cut(s) 439, 562, 1490, 1855
TseI GCWGC 6 cut(s) 649, 740, 1015, 1018, 1133, 1625
Tsp45I GTSAC 4 cut(s) 439, 562, 1490, 1855
TspDTI ATGAA 8 cut(s) 43, 470, 929, 1095, 1143, 1314, 1527, 1811
TspRI CASTG 5 cut(s) 148, 552, 853, 858, 894
Van91I CCANNNNNTGG 1 cut(s) 280
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 5 cut(s) 656, 942, 1263, 1423, 1783
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 1 cut(s) 1753
XceI RCATGY 3 cut(s) 738, 1158, 1336
XmiI GTMKAC 1 cut(s) 1189
XspI CTAG 5 cut(s) 960, 996, 1022, 1256, 1860
ZrmI AGTACT 1 cut(s) 840
Zsp2I ATGCAT 1 cut(s) 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.