MD12G1075200.v1.1

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006364
Physical Location & Seq
Forward (+)
9108198 .. 9111657
3460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1075200.v1.1.491

Sequence Viewer

Length: 1275 bp
ATGGCGTCGTCGTCCTCGGATCCCGGTCCGAAGACCGAAAGCGGTGGCGGCAGTGGCGGTGGGGGTGCAGAGCCGTCGGAGGCGGTGATGGCGAGCGATCAGCTAATGGTGTACAGAGGGGTAAAGAAATCGAAGAAAGAGAGAGGATGTACGGCGAAAGAGCGCATCAGCAAGATGCCTCCGTGTGCTGCTGGAAAACGCAGCTCCATTTACCGTGGAGTCACTCGGCATAGGTGGACAGGTCGGTATGAGGCTCATCTTTGGGATAAAAGTACTTGGAATCAGAACCAGAATAAGAAGGGAAAGCAAGTTTACTTGGGGGCCTATGACGATGAAGAGGCTGCAGCTAGAGCTTATGATCTTGCTGCCTTGAAATATTGGGGTCCTGGCACTCTCATCAATTTTCCTGTTACTGATTACACAAGGGATCTTGAAGAGATGCAGAATCTGTCAAGGGAGGAATACCTTGCTTCTCTTCGGAGGAAAAGCAGTGGTTTCTCAAGAGGACTTTCTAAGTACCGTGGGCTTTCCAGTCGTTGGGAGCCGTCACTGGGTCGTATGGGTGGATCTGAGTACTTCAATAGCTTACATTATGGTACAGGCGTTGATCCAGCAACAGAAAGTGAACTTTTGGGAGCTTTTTGCATTGAAAGAAAGATTGACCTAACAAGTTACATCAAGTGGTGGGGACCCAACAGATCTCGTCAAGCTGAAACTAGCATGAAATCGTCAGAAGAAACAAAACACGGTTGTGTTGGAGATATTGGAGCTGAACTTAAAACATTGGAATGGGAAATCCAGCCTACTGAACCATACCAGATGCCCCGTTTGGGCATCTCCCATGTAAGTAAAAAGCATAAAGGTACCAGAGTCTCAGCCATGAGCATCCTTTCAAGGTCAGCGGCATATAAGAACTTGCAAGAGAAAGCATCACAAAAGGAGGAAAAAGATGCAGATAATGATGAGAATGAAAACAAAAATACCATCCACAAGATGGACTATGGCAAAGCAGTTGAGAAATCCACAAGTCATGATGAGGGACTTAGTTCTGCATTAGGAATGAGTGGTGGACCATCTCACCAAAGAGATGTGTTCCCATTGGCTCCCTTCTTGTCTGCACCACTTCTGACCAGCTACAGTGCCATCGATCCCTTGGTAGACCCCATTCTCTGGACGTCTCTTGCTCCTGTTCTTCCTTCTGGAATTTCTCGTCCTACTGAGATTACAAAGACCGAGACTAGTTCAAGTTTCGCTTTATTTCAGCCCGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

425

Amino Acids

46.8

Weight (kDa)

8.42

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 71 - 128 2.7e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1178
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 863
AccB7I CCANNNNNTGG 3 cut(s) 537, 994, 1170
AccI GTMKAC 1 cut(s) 1158
AciI CCGC 5 cut(s) 42, 48, 57, 83, 902
AclWI GGATC 6 cut(s) 14, 27, 435, 574, 602, 1142
AcsI RAATTY 1 cut(s) 1203
AcyI GRCGYC 2 cut(s) 5, 1175
AfaI GTAC 7 cut(s) 113, 151, 274, 518, 575, 598, 865
AfiI CCNNNNNNNGG 5 cut(s) 537, 551, 830, 994, 1170
AgsI TTSAA 6 cut(s) 373, 434, 580, 650, 894, 1245
AhlI ACTAGT 1 cut(s) 1238
AjnI CCWGG 1 cut(s) 385
AleI CACNNNNGTG 1 cut(s) 750
AluBI AGCT 9 cut(s) 103, 204, 347, 353, 585, 638, 710, 770, 1134
AluI AGCT 9 cut(s) 103, 204, 347, 353, 585, 638, 710, 770, 1134
Alw26I GTCTC 3 cut(s) 877, 1182, 1229
AlwI GGATC 6 cut(s) 14, 27, 435, 574, 602, 1142
AlwNI CAGNNNCTG 1 cut(s) 448
AoxI GGCC 1 cut(s) 321
ApeKI GCWGC 5 cut(s) 188, 201, 341, 344, 365
ApoI RAATTY 1 cut(s) 1203
Asp718I GGTACC 1 cut(s) 863
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 5 cut(s) 26, 321, 383, 689, 1070
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 97, 1070
AvaII GGWCC 4 cut(s) 26, 383, 689, 1070
BaeI ACNNNNGTAYC 4 cut(s) 588, 621, 855, 888
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 863
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 5 cut(s) 175, 213, 328, 352, 356
BccI CCATC 5 cut(s) 82, 988, 992, 1081, 1151
BceAI ACGGC 3 cut(s) 58, 168, 529
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 3 cut(s) 877, 1182, 1229
BcuI ACTAGT 1 cut(s) 1238
BfaI CTAG 3 cut(s) 348, 717, 1239
BfmI CTRYAG 2 cut(s) 342, 1135
BglII AGATCT 1 cut(s) 698
BisI GCNGC 7 cut(s) 49, 189, 202, 342, 345, 366, 903
BlsI GCNGC 7 cut(s) 50, 190, 203, 343, 346, 367, 904
BmcAI AGTACT 2 cut(s) 274, 575
Bme1390I CCNGG 2 cut(s) 24, 387
Bme18I GGWCC 4 cut(s) 26, 383, 689, 1070
BmgT120I GGNCC 5 cut(s) 26, 321, 383, 689, 1070
BmiI GGNNCC 8 cut(s) 21, 322, 384, 543, 690, 691, 865, 1104
BmrFI CCNGG 2 cut(s) 24, 387
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 8 cut(s) 165, 174, 429, 810, 843, 894, 938, 940
BmuI ACTGGG 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 484
BpuMI CCSGG 1 cut(s) 24
Bsa29I ATCGAT 1 cut(s) 1146
BsaHI GRCGYC 2 cut(s) 5, 1175
BsaJI CCNNGG 4 cut(s) 15, 214, 520, 1152
Bsc4I CCNNNNNNNGG 5 cut(s) 537, 551, 830, 994, 1170
Bse1I ACTGG 2 cut(s) 531, 555
BseBI CCWGG 1 cut(s) 387
BseCI ATCGAT 1 cut(s) 1146
BseDI CCNNGG 4 cut(s) 15, 214, 520, 1152
BseGI GGATG 3 cut(s) 152, 885, 984
BseLI CCNNNNNNNGG 5 cut(s) 537, 551, 830, 994, 1170
BseMII CTCAG 3 cut(s) 561, 888, 1209
BseNI ACTGG 2 cut(s) 531, 555
BseXI GCAGC 5 cut(s) 175, 213, 328, 352, 356
BsgI GTGCAG 2 cut(s) 87, 1101
BshFI GGCC 1 cut(s) 323
BshNI GGYRCC 1 cut(s) 863
BshVI ATCGAT 1 cut(s) 1146
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 2 cut(s) 702, 1053
BslI CCNNNNNNNGG 5 cut(s) 537, 551, 830, 994, 1170
BsmAI GTCTC 3 cut(s) 877, 1182, 1229
BsmBI CGTCTC 1 cut(s) 1182
BsmFI GGGAC 2 cut(s) 702, 1053
BsnI GGCC 1 cut(s) 323
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1147
BspACI CCGC 5 cut(s) 42, 48, 57, 83, 902
BspANI GGCC 1 cut(s) 323
BspCNI CTCAG 3 cut(s) 562, 887, 1210
BspDI ATCGAT 1 cut(s) 1146
BspHI TCATGA 1 cut(s) 1030
BspLI GGNNCC 8 cut(s) 21, 322, 384, 543, 690, 691, 865, 1104
BspMAI CTGCAG 1 cut(s) 346
BspPI GGATC 6 cut(s) 14, 27, 435, 574, 602, 1142
BspT107I GGYRCC 1 cut(s) 863
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 2 cut(s) 531, 555
BssECI CCNNGG 4 cut(s) 15, 214, 520, 1152
BssMI GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1147
BssNI GRCGYC 2 cut(s) 5, 1175
BssT1I CCWWGG 1 cut(s) 1152
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 4 cut(s) 215, 521, 749, 1139
Bst6I CTCTTC 3 cut(s) 330, 429, 480
BstACI GRCGYC 2 cut(s) 5, 1175
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 5 cut(s) 513, 570, 874, 1043, 1218
BstDSI CCRYGG 2 cut(s) 214, 520
BstF5I GGATG 3 cut(s) 152, 885, 984
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 8 cut(s) 22, 100, 361, 430, 569, 610, 701, 1150
BstMAI GTCTC 3 cut(s) 877, 1182, 1229
BstMBI GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 48, 54, 89, 350
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 22, 385
BstSFI CTRYAG 2 cut(s) 342, 1135
BstV1I GCAGC 5 cut(s) 175, 213, 328, 352, 356
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 4 cut(s) 19, 427, 566, 698
BstYI RGATCY 4 cut(s) 19, 427, 566, 698
Bsu15I ATCGAT 1 cut(s) 1146
BsuRI GGCC 1 cut(s) 323
BsuTUI ATCGAT 1 cut(s) 1146
BtgI CCRYGG 2 cut(s) 214, 520
BtsCI GGATG 3 cut(s) 152, 885, 984
BtsI GCAGTG 2 cut(s) 58, 496
BtsIMutI CAGTG 4 cut(s) 58, 496, 548, 1144
Cac8I GCNNGC 1 cut(s) 94
CaiI CAGNNNCTG 1 cut(s) 448
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 5 cut(s) 26, 321, 383, 689, 1070
ClaI ATCGAT 1 cut(s) 1146
CpoI CGGWCCG 1 cut(s) 26
Csp6I GTAC 7 cut(s) 112, 150, 273, 517, 574, 597, 864
CspI CGGWCCG 1 cut(s) 26
CviAII CATG 4 cut(s) 721, 842, 880, 1031
CviQI GTAC 7 cut(s) 112, 150, 273, 517, 574, 597, 864
DdeI CTNAG 5 cut(s) 513, 570, 874, 1043, 1218
DpnI GATC 8 cut(s) 21, 99, 360, 429, 568, 609, 700, 1149
DpnII GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1147
Eam1104I CTCTTC 3 cut(s) 330, 429, 480
EarI CTCTTC 3 cut(s) 330, 429, 480
Eco130I CCWWGG 1 cut(s) 1152
Eco47I GGWCC 4 cut(s) 26, 383, 689, 1070
EcoO109I RGGNCCY 3 cut(s) 321, 383, 689
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 1152
ErhI CCWWGG 1 cut(s) 1152
Esp3I CGTCTC 1 cut(s) 1182
FaeI CATG 4 cut(s) 724, 845, 883, 1034
FalI AAGNNNNNCTT 2 cut(s) 1237, 1269
FaqI GGGAC 2 cut(s) 702, 1053
FatI CATG 4 cut(s) 720, 841, 879, 1030
FblI GTMKAC 1 cut(s) 1158
Fnu4HI GCNGC 7 cut(s) 49, 189, 202, 342, 345, 366, 903
FokI GGATG 3 cut(s) 159, 872, 971
Fsp4HI GCNGC 7 cut(s) 49, 189, 202, 342, 345, 366, 903
FspBI CTAG 3 cut(s) 348, 717, 1239
GlaI GCGC 1 cut(s) 164
GluI GCNGC 7 cut(s) 49, 189, 202, 342, 345, 366, 903
HaeIII GGCC 1 cut(s) 323
HapII CCGG 1 cut(s) 24
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 2 cut(s) 5, 1175
Hin1II CATG 4 cut(s) 724, 845, 883, 1034
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 4 cut(s) 219, 280, 445, 870
HpaII CCGG 1 cut(s) 24
HphI GGTGA 2 cut(s) 97, 1070
Hpy166II GTNNAC 6 cut(s) 112, 237, 313, 626, 1070, 1159
Hpy188I TCNGA 8 cut(s) 19, 30, 79, 285, 480, 571, 733, 1128
Hpy188III TCNNGA 5 cut(s) 431, 501, 1031, 1171, 1200
Hpy8I GTNNAC 6 cut(s) 112, 237, 313, 626, 1070, 1159
Hpy99I CGWCG 3 cut(s) 10, 13, 79
HpyAV CCTTC 3 cut(s) 292, 1117, 1206
HpyCH4III ACNGT 4 cut(s) 215, 521, 749, 1139
HpyCH4IV ACGT 1 cut(s) 1175
HpyCH4V TGCA 8 cut(s) 68, 344, 442, 645, 919, 953, 1052, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 54, 89, 350
HpyF3I CTNAG 5 cut(s) 513, 570, 874, 1043, 1218
HpySE526I ACGT 1 cut(s) 1175
Hsp92I GRCGYC 2 cut(s) 5, 1175
Hsp92II CATG 4 cut(s) 724, 845, 883, 1034
HspAI GCGC 1 cut(s) 163
KflI GGGWCCC 1 cut(s) 689
KpnI GGTACC 1 cut(s) 867
Kzo9I GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1147
LmnI GCTCC 6 cut(s) 209, 541, 635, 767, 1108, 1189
Lsp1109I GCAGC 5 cut(s) 175, 213, 328, 352, 356
LweI GCATC 8 cut(s) 165, 174, 429, 810, 843, 894, 938, 940
MaeI CTAG 3 cut(s) 348, 717, 1239
MaeII ACGT 1 cut(s) 1175
MaeIII GTNAC 4 cut(s) 220, 409, 546, 671
MalI GATC 8 cut(s) 21, 99, 360, 429, 568, 609, 700, 1149
MboI GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1147
MboII GAAGA 7 cut(s) 43, 145, 347, 446, 467, 746, 1184
MflI RGATCY 4 cut(s) 19, 427, 566, 698
MluCI AATT 2 cut(s) 400, 1203
MlyI GAGTC 2 cut(s) 228, 879
MmeI TCCRAC 2 cut(s) 57, 736
MseI TTAA 1 cut(s) 777
MslI CAYNNNNRTG 2 cut(s) 750, 787
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 2 cut(s) 24, 387
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 4 cut(s) 48, 54, 89, 350
NciI CCSGG 1 cut(s) 24
NdeII GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1147
NlaIII CATG 4 cut(s) 724, 845, 883, 1034
NlaIV GGNNCC 8 cut(s) 21, 322, 384, 543, 690, 691, 865, 1104
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 220, 546
OliI CACNNNNGTG 1 cut(s) 750
PagI TCATGA 1 cut(s) 1030
PfeI GAWTC 2 cut(s) 280, 445
PflMI CCANNNNNTGG 3 cut(s) 537, 994, 1170
PkrI GCNGC 7 cut(s) 50, 190, 203, 343, 346, 367, 904
PleI GAGTC 2 cut(s) 227, 878
PpsI GAGTC 2 cut(s) 227, 878
PpuMI RGGWCCY 2 cut(s) 383, 689
Psp5II RGGWCCY 2 cut(s) 383, 689
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 8 cut(s) 21, 322, 384, 543, 690, 691, 865, 1104
PspPI GGNCC 5 cut(s) 26, 321, 383, 689, 1070
PspPPI RGGWCCY 2 cut(s) 383, 689
PstI CTGCAG 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 448
PsuI RGATCY 4 cut(s) 19, 427, 566, 698
RsaI GTAC 7 cut(s) 113, 151, 274, 518, 575, 598, 865
RsaNI GTAC 7 cut(s) 112, 150, 273, 517, 574, 597, 864
RseI CAYNNNNRTG 2 cut(s) 750, 787
Rsr2I CGGWCCG 1 cut(s) 26
RsrII CGGWCCG 1 cut(s) 26
SaqAI TTAA 1 cut(s) 777
SatI GCNGC 7 cut(s) 49, 189, 202, 342, 345, 366, 903
Sau3AI GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1147
Sau96I GGNCC 5 cut(s) 26, 321, 383, 689, 1070
ScaI AGTACT 2 cut(s) 274, 575
SchI GAGTC 2 cut(s) 228, 879
ScrFI CCNGG 2 cut(s) 24, 387
SfaNI GCATC 8 cut(s) 165, 174, 429, 810, 843, 894, 938, 940
SfcI CTRYAG 2 cut(s) 342, 1135
SinI GGWCC 4 cut(s) 26, 383, 689, 1070
SmiMI CAYNNNNRTG 2 cut(s) 750, 787
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
SpeI ACTAGT 1 cut(s) 1238
Sse9I AATT 2 cut(s) 400, 1203
SsiI CCGC 5 cut(s) 42, 48, 57, 83, 902
SspI AATATT 1 cut(s) 377
SspMI CTAG 3 cut(s) 348, 717, 1239
StyD4I CCNGG 2 cut(s) 22, 385
StyI CCWWGG 1 cut(s) 1152
TaaI ACNGT 4 cut(s) 215, 521, 749, 1139
TaiI ACGT 1 cut(s) 1178
TaqI TCGA 2 cut(s) 131, 1146
TaqII GACCGA 2 cut(s) 50, 1247
TasI AATT 2 cut(s) 400, 1203
TatI WGTACW 3 cut(s) 111, 272, 573
TauI GCSGC 2 cut(s) 51, 905
TfiI GAWTC 2 cut(s) 280, 445
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TscAI CASTG 4 cut(s) 58, 496, 555, 1144
TseFI GTSAC 2 cut(s) 220, 546
TseI GCWGC 5 cut(s) 188, 201, 341, 344, 365
Tsp45I GTSAC 2 cut(s) 220, 546
TspDTI ATGAA 3 cut(s) 348, 737, 984
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 4 cut(s) 58, 496, 555, 1144
Van91I CCANNNNNTGG 3 cut(s) 537, 994, 1170
VpaK11BI GGWCC 4 cut(s) 26, 383, 689, 1070
XapI RAATTY 1 cut(s) 1203
XcmI CCANNNNNNNNNTGG 2 cut(s) 991, 1150
XmiI GTMKAC 1 cut(s) 1158
XspI CTAG 3 cut(s) 348, 717, 1239
ZraI GACGTC 1 cut(s) 1176
ZrmI AGTACT 2 cut(s) 274, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.