pycom12g06470

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006364
Physical Location & Seq
Forward (+)
6667789 .. 6670927
3139 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06470.1

Sequence Viewer

Length: 1272 bp
ATGGCGTCGTCGTCCTCGGATCCCGGTCCGAAGACCGAAAGCGGCGGCGGCAGTGGCGGTGGGGGTGCAGAGCCGTCGGAGGCGGTGATGGCGAGCGATCAGCTAATGGTGTACAGAGGGGTAAAGAAATCGAAGAAAGAGAGAGGATGTACGGCGAAAGAGCGCATCAGCAAGATGCCTCCGTGTGCTGCTGGAAAACGCAGCTCCATTTACCGTGGAGTCACTCGGCATAGGTGGACAGGTCGTTATGAGGCTCATCTTTGGGATAAAAGTACCTGGAATCAGAACCAGAATAAGAAGGGAAAGCAAGTTTACTTGGGGGCGTATGACGATGAAGAGGCTGCAGCTAGAGCTTATGATCTTGCTGCCTTGAAATATTGGGGCCCTGGCACTCTCATCAATTTTCCTGTTACTGATTACACAAGAGATCTTGAAGAGATGCAGAATCTGTCAAGGGAGGAATACCTTGCTTCTCTTCGGAGGAAAAGCAGTGGTTTCTCAAGAGGACTTTCTAAGTACCGTGGGCTTTCCAGTCGTTGGGAGCCGTCACTGGGTCGTATGGGTGGATCTGAGTACTTCAATAGCTTACATTATGGTACAGGCGTTGATCCAGCAACAGAAAGTGAACTTTTGGGAGCTTTTTGCACTGAAAGAAAGATTGACCTAACAAGTTACATCAAGTGGTGGGGACCCAACAGATCTCGTCAAGCTGAAACTAGCATGAAATCGTCAGAAGAAACAAAACACGGTTGTGTTGGAGATATTGGAGCTGAACTTAAAACATTGGAATGGGAAATCCAGCCTACTGAACCATACCAGATGCCCTGTTTGGGCATCTCCCATGTAAGTAAAAAGCATAAAGGTACCAGAGTCTCAGCCATGAGCATCCTTTCAAGGTCAGCGGCATATAAGAACTTGCAAGAGAAAGCATCACAAAAGGAGGAAAAAGATGCAGATAATGATGAGAATGAAAACAAAAATACCATCCACAAGATGGACCATGGCAAAGCAGTTGAGAAATCCACAAGTCATGATGAGGGACTTAGGTCTGCATTAGGAATGAGTGGACCGTCTCACCAAAGAGATGTGTTCCCATTGGCTCCCTTCTTGTCTGCACCACTTCTGACCAGCTACAGTGCCATCGATCCCTTGGTAGACCCCATTCTCTGGACGTCTCTTGCTCCTGTTCTTCCTTCTGGAATTTCTCGTCCTACTGAGATTACAAAGACTGAGACTAGTTCAAGTTTCGCTTTATTTCAGCCCGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

424

Amino Acids

46.72

Weight (kDa)

8.38

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 71 - 128 2.7e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1175
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 863
AccB7I CCANNNNNTGG 3 cut(s) 537, 994, 1167
AccI GTMKAC 1 cut(s) 1155
AciI CCGC 6 cut(s) 42, 45, 48, 57, 83, 902
AclWI GGATC 5 cut(s) 14, 27, 574, 602, 1139
AcsI RAATTY 1 cut(s) 1200
AcyI GRCGYC 2 cut(s) 5, 1172
AfaI GTAC 7 cut(s) 113, 151, 274, 518, 575, 598, 865
AfiI CCNNNNNNNGG 5 cut(s) 537, 551, 830, 994, 1167
AgsI TTSAA 5 cut(s) 373, 434, 580, 894, 1242
AhlI ACTAGT 1 cut(s) 1235
AjnI CCWGG 2 cut(s) 275, 385
AleI CACNNNNGTG 1 cut(s) 750
AluBI AGCT 9 cut(s) 103, 204, 347, 353, 585, 638, 710, 770, 1131
AluI AGCT 9 cut(s) 103, 204, 347, 353, 585, 638, 710, 770, 1131
Alw26I GTCTC 4 cut(s) 877, 1077, 1179, 1226
AlwI GGATC 5 cut(s) 14, 27, 574, 602, 1139
AlwNI CAGNNNCTG 1 cut(s) 448
AoxI GGCC 1 cut(s) 382
ApaI GGGCCC 1 cut(s) 386
ApeKI GCWGC 5 cut(s) 188, 201, 341, 344, 365
ApoI RAATTY 1 cut(s) 1200
Asp718I GGTACC 1 cut(s) 863
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 6 cut(s) 26, 382, 383, 689, 997, 1067
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 97, 1067
AvaII GGWCC 4 cut(s) 26, 689, 997, 1067
BaeGI GKGCMC 1 cut(s) 386
BaeI ACNNNNGTAYC 4 cut(s) 588, 621, 855, 888
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 1 cut(s) 386
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 5 cut(s) 175, 213, 328, 352, 356
BccI CCATC 4 cut(s) 82, 988, 992, 1148
BceAI ACGGC 3 cut(s) 58, 168, 529
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciT130I CCWGG 2 cut(s) 277, 387
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 4 cut(s) 877, 1077, 1179, 1226
BcuI ACTAGT 1 cut(s) 1235
BfaI CTAG 3 cut(s) 348, 717, 1236
BfmI CTRYAG 2 cut(s) 342, 1132
BglII AGATCT 2 cut(s) 427, 698
BisI GCNGC 9 cut(s) 43, 46, 49, 189, 202, 342, 345, 366, 903
BlsI GCNGC 9 cut(s) 44, 47, 50, 190, 203, 343, 346, 367, 904
BmcAI AGTACT 1 cut(s) 575
Bme1390I CCNGG 3 cut(s) 24, 277, 387
Bme18I GGWCC 4 cut(s) 26, 689, 997, 1067
BmgT120I GGNCC 6 cut(s) 26, 382, 383, 689, 997, 1067
BmiI GGNNCC 8 cut(s) 21, 383, 384, 543, 690, 691, 865, 1101
BmrFI CCNGG 3 cut(s) 24, 277, 387
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 8 cut(s) 165, 174, 429, 810, 843, 894, 938, 940
BmuI ACTGGG 1 cut(s) 560
BoxI GACNNNNGTC 1 cut(s) 1045
BpiI GAAGAC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 484
BpuMI CCSGG 1 cut(s) 24
Bsa29I ATCGAT 1 cut(s) 1143
BsaHI GRCGYC 2 cut(s) 5, 1172
BsaJI CCNNGG 6 cut(s) 15, 214, 385, 520, 1000, 1149
Bsc4I CCNNNNNNNGG 5 cut(s) 537, 551, 830, 994, 1167
Bse1I ACTGG 2 cut(s) 531, 555
BseBI CCWGG 2 cut(s) 277, 387
BseCI ATCGAT 1 cut(s) 1143
BseDI CCNNGG 6 cut(s) 15, 214, 385, 520, 1000, 1149
BseGI GGATG 3 cut(s) 152, 885, 984
BseLI CCNNNNNNNGG 5 cut(s) 537, 551, 830, 994, 1167
BseMII CTCAG 4 cut(s) 561, 888, 1206, 1221
BseNI ACTGG 2 cut(s) 531, 555
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 5 cut(s) 175, 213, 328, 352, 356
BsgI GTGCAG 2 cut(s) 87, 1098
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 863
BshVI ATCGAT 1 cut(s) 1143
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 2 cut(s) 702, 1053
BslI CCNNNNNNNGG 5 cut(s) 537, 551, 830, 994, 1167
BsmAI GTCTC 4 cut(s) 877, 1077, 1179, 1226
BsmBI CGTCTC 2 cut(s) 1077, 1179
BsmFI GGGAC 2 cut(s) 702, 1053
BsnI GGCC 1 cut(s) 384
Bsp120I GGGCCC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 386
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1144
Bsp19I CCATGG 1 cut(s) 1000
BspACI CCGC 6 cut(s) 42, 45, 48, 57, 83, 902
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 4 cut(s) 562, 887, 1207, 1222
BspDI ATCGAT 1 cut(s) 1143
BspHI TCATGA 1 cut(s) 1030
BspLI GGNNCC 8 cut(s) 21, 383, 384, 543, 690, 691, 865, 1101
BspMAI CTGCAG 1 cut(s) 346
BspPI GGATC 5 cut(s) 14, 27, 574, 602, 1139
BspT107I GGYRCC 1 cut(s) 863
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 2 cut(s) 531, 555
BssECI CCNNGG 6 cut(s) 15, 214, 385, 520, 1000, 1149
BssMI GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1144
BssNI GRCGYC 2 cut(s) 5, 1172
BssT1I CCWWGG 2 cut(s) 1000, 1149
Bst2UI CCWGG 2 cut(s) 277, 387
Bst4CI ACNGT 5 cut(s) 215, 521, 749, 1071, 1136
Bst6I CTCTTC 3 cut(s) 330, 429, 480
BstACI GRCGYC 2 cut(s) 5, 1172
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 6 cut(s) 513, 570, 874, 1043, 1215, 1230
BstDSI CCRYGG 3 cut(s) 214, 520, 1000
BstF5I GGATG 3 cut(s) 152, 885, 984
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 8 cut(s) 22, 100, 361, 430, 569, 610, 701, 1147
BstMAI GTCTC 4 cut(s) 877, 1077, 1179, 1226
BstMBI GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1144
BstMWI GCNNNNNNNGC 4 cut(s) 48, 54, 89, 350
BstNI CCWGG 2 cut(s) 277, 387
BstPAI GACNNNNGTC 1 cut(s) 1045
BstSCI CCNGG 3 cut(s) 22, 275, 385
BstSFI CTRYAG 2 cut(s) 342, 1132
BstSLI GKGCMC 1 cut(s) 386
BstV1I GCAGC 5 cut(s) 175, 213, 328, 352, 356
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 4 cut(s) 19, 427, 566, 698
BstYI RGATCY 4 cut(s) 19, 427, 566, 698
Bsu15I ATCGAT 1 cut(s) 1143
BsuRI GGCC 1 cut(s) 384
BsuTUI ATCGAT 1 cut(s) 1143
BtgI CCRYGG 3 cut(s) 214, 520, 1000
BtsCI GGATG 3 cut(s) 152, 885, 984
BtsI GCAGTG 2 cut(s) 58, 496
BtsIMutI CAGTG 5 cut(s) 58, 496, 548, 645, 1141
Cac8I GCNNGC 1 cut(s) 94
CaiI CAGNNNCTG 1 cut(s) 448
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 6 cut(s) 26, 382, 383, 689, 997, 1067
ClaI ATCGAT 1 cut(s) 1143
CpoI CGGWCCG 1 cut(s) 26
Csp6I GTAC 7 cut(s) 112, 150, 273, 517, 574, 597, 864
CspI CGGWCCG 1 cut(s) 26
CviAII CATG 5 cut(s) 721, 842, 880, 1001, 1031
CviQI GTAC 7 cut(s) 112, 150, 273, 517, 574, 597, 864
DdeI CTNAG 6 cut(s) 513, 570, 874, 1043, 1215, 1230
DpnI GATC 8 cut(s) 21, 99, 360, 429, 568, 609, 700, 1146
DpnII GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1144
Eam1104I CTCTTC 3 cut(s) 330, 429, 480
EarI CTCTTC 3 cut(s) 330, 429, 480
Eco130I CCWWGG 2 cut(s) 1000, 1149
Eco24I GRGCYC 1 cut(s) 386
Eco47I GGWCC 4 cut(s) 26, 689, 997, 1067
EcoO109I RGGNCCY 3 cut(s) 382, 383, 689
EcoRII CCWGG 2 cut(s) 275, 385
EcoT14I CCWWGG 2 cut(s) 1000, 1149
EcoT38I GRGCYC 1 cut(s) 386
ErhI CCWWGG 2 cut(s) 1000, 1149
Esp3I CGTCTC 2 cut(s) 1077, 1179
FaeI CATG 5 cut(s) 724, 845, 883, 1004, 1034
FalI AAGNNNNNCTT 2 cut(s) 1234, 1266
FaqI GGGAC 2 cut(s) 702, 1053
FatI CATG 5 cut(s) 720, 841, 879, 1000, 1030
FblI GTMKAC 1 cut(s) 1155
Fnu4HI GCNGC 9 cut(s) 43, 46, 49, 189, 202, 342, 345, 366, 903
FokI GGATG 3 cut(s) 159, 872, 971
FriOI GRGCYC 1 cut(s) 386
Fsp4HI GCNGC 9 cut(s) 43, 46, 49, 189, 202, 342, 345, 366, 903
FspBI CTAG 3 cut(s) 348, 717, 1236
GlaI GCGC 1 cut(s) 164
GluI GCNGC 9 cut(s) 43, 46, 49, 189, 202, 342, 345, 366, 903
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 24
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 2 cut(s) 5, 1172
Hin1II CATG 5 cut(s) 724, 845, 883, 1004, 1034
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 4 cut(s) 219, 280, 445, 870
HpaII CCGG 1 cut(s) 24
HphI GGTGA 2 cut(s) 97, 1067
Hpy166II GTNNAC 6 cut(s) 112, 237, 313, 626, 1067, 1156
Hpy188I TCNGA 8 cut(s) 19, 30, 79, 285, 480, 571, 733, 1125
Hpy188III TCNNGA 5 cut(s) 431, 501, 1031, 1168, 1197
Hpy8I GTNNAC 6 cut(s) 112, 237, 313, 626, 1067, 1156
Hpy99I CGWCG 3 cut(s) 10, 13, 79
HpyAV CCTTC 3 cut(s) 292, 1114, 1203
HpyCH4III ACNGT 5 cut(s) 215, 521, 749, 1071, 1136
HpyCH4IV ACGT 1 cut(s) 1172
HpyCH4V TGCA 8 cut(s) 68, 344, 442, 645, 919, 953, 1052, 1115
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 54, 89, 350
HpyF3I CTNAG 6 cut(s) 513, 570, 874, 1043, 1215, 1230
HpySE526I ACGT 1 cut(s) 1172
Hsp92I GRCGYC 2 cut(s) 5, 1172
Hsp92II CATG 5 cut(s) 724, 845, 883, 1004, 1034
HspAI GCGC 1 cut(s) 163
KflI GGGWCCC 1 cut(s) 689
KpnI GGTACC 1 cut(s) 867
Kzo9I GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1144
LmnI GCTCC 6 cut(s) 209, 541, 635, 767, 1105, 1186
Lsp1109I GCAGC 5 cut(s) 175, 213, 328, 352, 356
LweI GCATC 8 cut(s) 165, 174, 429, 810, 843, 894, 938, 940
MaeI CTAG 3 cut(s) 348, 717, 1236
MaeII ACGT 1 cut(s) 1172
MaeIII GTNAC 4 cut(s) 220, 409, 546, 671
MalI GATC 8 cut(s) 21, 99, 360, 429, 568, 609, 700, 1146
MboI GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1144
MboII GAAGA 7 cut(s) 43, 145, 347, 446, 467, 746, 1181
MflI RGATCY 4 cut(s) 19, 427, 566, 698
MhlI GDGCHC 1 cut(s) 386
MluCI AATT 2 cut(s) 400, 1200
MlyI GAGTC 2 cut(s) 228, 879
MmeI TCCRAC 2 cut(s) 57, 736
MseI TTAA 1 cut(s) 777
MslI CAYNNNNRTG 2 cut(s) 750, 787
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 3 cut(s) 24, 277, 387
MvaI CCWGG 2 cut(s) 277, 387
MwoI GCNNNNNNNGC 4 cut(s) 48, 54, 89, 350
NciI CCSGG 1 cut(s) 24
NcoI CCATGG 1 cut(s) 1000
NdeII GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1144
NlaIII CATG 5 cut(s) 724, 845, 883, 1004, 1034
NlaIV GGNNCC 8 cut(s) 21, 383, 384, 543, 690, 691, 865, 1101
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 220, 546
OliI CACNNNNGTG 1 cut(s) 750
PagI TCATGA 1 cut(s) 1030
PfeI GAWTC 2 cut(s) 280, 445
PflMI CCANNNNNTGG 3 cut(s) 537, 994, 1167
PkrI GCNGC 9 cut(s) 44, 47, 50, 190, 203, 343, 346, 367, 904
PleI GAGTC 2 cut(s) 227, 878
PpsI GAGTC 2 cut(s) 227, 878
PpuMI RGGWCCY 1 cut(s) 689
PshAI GACNNNNGTC 1 cut(s) 1045
Psp5II RGGWCCY 1 cut(s) 689
Psp6I CCWGG 2 cut(s) 275, 385
PspGI CCWGG 2 cut(s) 275, 385
PspN4I GGNNCC 8 cut(s) 21, 383, 384, 543, 690, 691, 865, 1101
PspOMI GGGCCC 1 cut(s) 382
PspPI GGNCC 6 cut(s) 26, 382, 383, 689, 997, 1067
PspPPI RGGWCCY 1 cut(s) 689
PstI CTGCAG 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 448
PsuI RGATCY 4 cut(s) 19, 427, 566, 698
RsaI GTAC 7 cut(s) 113, 151, 274, 518, 575, 598, 865
RsaNI GTAC 7 cut(s) 112, 150, 273, 517, 574, 597, 864
RseI CAYNNNNRTG 2 cut(s) 750, 787
Rsr2I CGGWCCG 1 cut(s) 26
RsrII CGGWCCG 1 cut(s) 26
SaqAI TTAA 1 cut(s) 777
SatI GCNGC 9 cut(s) 43, 46, 49, 189, 202, 342, 345, 366, 903
Sau3AI GATC 8 cut(s) 19, 97, 358, 427, 566, 607, 698, 1144
Sau96I GGNCC 6 cut(s) 26, 382, 383, 689, 997, 1067
ScaI AGTACT 1 cut(s) 575
SchI GAGTC 2 cut(s) 228, 879
ScrFI CCNGG 3 cut(s) 24, 277, 387
SduI GDGCHC 1 cut(s) 386
SfaNI GCATC 8 cut(s) 165, 174, 429, 810, 843, 894, 938, 940
SfcI CTRYAG 2 cut(s) 342, 1132
SinI GGWCC 4 cut(s) 26, 689, 997, 1067
SmiMI CAYNNNNRTG 2 cut(s) 750, 787
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
SpeI ACTAGT 1 cut(s) 1235
Sse9I AATT 2 cut(s) 400, 1200
SsiI CCGC 6 cut(s) 42, 45, 48, 57, 83, 902
SspI AATATT 1 cut(s) 377
SspMI CTAG 3 cut(s) 348, 717, 1236
StyD4I CCNGG 3 cut(s) 22, 275, 385
StyI CCWWGG 2 cut(s) 1000, 1149
TaaI ACNGT 5 cut(s) 215, 521, 749, 1071, 1136
TaiI ACGT 1 cut(s) 1175
TaqI TCGA 2 cut(s) 131, 1143
TaqII GACCGA 1 cut(s) 50
TasI AATT 2 cut(s) 400, 1200
TatI WGTACW 2 cut(s) 111, 573
TauI GCSGC 4 cut(s) 45, 48, 51, 905
TfiI GAWTC 2 cut(s) 280, 445
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TscAI CASTG 5 cut(s) 58, 496, 555, 652, 1141
TseFI GTSAC 2 cut(s) 220, 546
TseI GCWGC 5 cut(s) 188, 201, 341, 344, 365
Tsp45I GTSAC 2 cut(s) 220, 546
TspDTI ATGAA 3 cut(s) 348, 737, 984
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 5 cut(s) 58, 496, 555, 652, 1141
Van91I CCANNNNNTGG 3 cut(s) 537, 994, 1167
VpaK11BI GGWCC 4 cut(s) 26, 689, 997, 1067
XapI RAATTY 1 cut(s) 1200
XcmI CCANNNNNNNNNTGG 2 cut(s) 991, 1147
XmiI GTMKAC 1 cut(s) 1155
XspI CTAG 3 cut(s) 348, 717, 1236
ZraI GACGTC 1 cut(s) 1173
ZrmI AGTACT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.