Rh3CG282300

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0006364
Physical Location & Seq
Forward (+)
28419700 .. 28423419
3720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG282300.1

Sequence Viewer

Length: 1002 bp
ATGGCTTCGTCGTCCTCGGATCCTGGTCCGAAGACCGAAAGCGGAAGCGGCAGCGGCGGCGGAGCGGAGGCTGCGGCGGCGGTGGCGACCGATCAGCAATTGCTTTACAGAGGGCTGAAGAAAGCGAAGAAAGAGAGAGGTTGCACGGCCAAAGAGCGCATCAGCAAAATGCCTCCCTGTGCCGCTGGAAAACGGAGCTCCATTTACCGTGGAGTCACTCGGAAGAGCAGTGGCTTCTCTAGAGGAATGTCTAAATATCGCGCGCTCTCAAGCCGTTGGGAGCCATCATTTGGTCGTATGGGTGGATCTGAATACTTCAATAGCATCCATTATGGCACAGGAGATGATCCAGCAACAGACAATGAATTTTTAGGAACGTTTTGCATTGAAAGAAAGATTGATTTAACAAGTTACATCAAGTGGTGGGGGCCTAACAAAACTCGTCAAGCGGACAGCATGATAAAATCATCTGAGGAAGCAAAACATGGTTTTGCTGGAGATAGTGGAGGTGAACTTAAAACATTGGAATGGGAATCCCAGCCTACTGAACCATACCAGATGCCACGTTTGGGTGTCTGCCGTGAAGGTAAAAAGCAAAATGGTGCCAGAGTCTCTGCCATGAGCATCTTGTCACGGTCACCTGCTTATAAGAGCTTACAAGAGAAAGCATCAATTAAGCAAGAAAGGAATGCAGATAATGACGAGAATGAAAACAAAAATACGATCCACAAAAAGGACTATGGCAAGGCAACTGAGAAATCCACAAGTCATGATGCTGGGGATGAGAGACTGAATGCTTCATTAGGAATGAGTGGTGGATTGTCTCTCCAGAGGAATGCATTCCCATTGGCTCCGTTCTTGTCTGCGCCACTGCTGACCAACTACAACATGATTGATCCATTAGTAGACCCCATTCTTTGGACATCTCTTGCTCCTGTTCTTCCCGAGGTTACAAAGACTGAGACCAGTGCAAGTTTCAGTTTATTTCGGCCTGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

333

Amino Acids

36.3

Weight (kDa)

9.06

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AarI CACCTGC 1 cut(s) 649
Acc36I ACCTGC 1 cut(s) 649
AccB1I GGYRCC 1 cut(s) 602
AccB7I CCANNNNNTGG 2 cut(s) 290, 918
AccBSI CCGCTC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 906
AccII CGCG 2 cut(s) 261, 263
AclI AACGTT 1 cut(s) 377
AclWI GGATC 6 cut(s) 14, 27, 313, 341, 718, 890
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 137
AfiI CCNNNNNNNGG 4 cut(s) 290, 569, 733, 918
AgsI TTSAA 2 cut(s) 319, 389
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 2 cut(s) 198, 654
AluI AGCT 2 cut(s) 198, 654
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 4 cut(s) 616, 781, 828, 956
AlwI GGATC 6 cut(s) 14, 27, 313, 341, 718, 890
Ama87I CYCGRG 1 cut(s) 944
AoxI GGCC 3 cut(s) 147, 428, 989
ApeKI GCWGC 2 cut(s) 51, 71
ApoI RAATTY 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 839
AspLEI GCGC 4 cut(s) 159, 263, 265, 868
AspS9I GGNCC 2 cut(s) 26, 428
AsuHPI GGTGA 2 cut(s) 521, 630
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 1 cut(s) 26
AxyI CCTNAGG 1 cut(s) 993
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 602
BanII GRGCYC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 2 cut(s) 58, 63
BccI CCATC 1 cut(s) 292
BceAI ACGGC 3 cut(s) 162, 258, 564
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 4 cut(s) 616, 781, 828, 956
BfaI CTAG 1 cut(s) 240
BfuAI ACCTGC 1 cut(s) 649
BisI GCNGC 8 cut(s) 49, 52, 55, 58, 72, 75, 78, 183
BlsI GCNGC 8 cut(s) 50, 53, 56, 59, 73, 76, 79, 184
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 944
BmgT120I GGNCC 2 cut(s) 26, 428
BmiI GGNNCC 5 cut(s) 21, 282, 429, 604, 852
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 6 cut(s) 168, 333, 549, 633, 677, 763
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 2 cut(s) 516, 812
BpuEI CTTGAG 1 cut(s) 253
BsaI GGTCTC 1 cut(s) 956
BsaJI CCNNGG 3 cut(s) 15, 208, 945
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 569, 733, 918
Bse1I ACTGG 1 cut(s) 966
Bse21I CCTNAGG 1 cut(s) 993
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 3 cut(s) 15, 208, 945
BseGI GGATG 2 cut(s) 324, 787
BseLI CCNNNNNNNGG 4 cut(s) 290, 569, 733, 918
BseMII CTCAG 4 cut(s) 462, 744, 951, 984
BseNI ACTGG 1 cut(s) 966
BsePI GCGCGC 1 cut(s) 261
BseXI GCAGC 2 cut(s) 58, 63
BseYI CCCAGC 2 cut(s) 537, 776
Bsh1236I CGCG 2 cut(s) 261, 263
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 149, 430, 991
BshNI GGYRCC 1 cut(s) 602
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 944
BslI CCNNNNNNNGG 4 cut(s) 290, 569, 733, 918
BsmAI GTCTC 4 cut(s) 616, 781, 828, 956
BsmI GAATGC 4 cut(s) 694, 799, 839, 841
BsnI GGCC 3 cut(s) 149, 430, 991
Bso31I GGTCTC 1 cut(s) 956
BsoBI CYCGRG 1 cut(s) 944
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 6 cut(s) 19, 91, 305, 346, 723, 895
BspANI GGCC 3 cut(s) 149, 430, 991
BspCNI CTCAG 4 cut(s) 463, 745, 952, 985
BspFNI CGCG 2 cut(s) 261, 263
BspHI TCATGA 1 cut(s) 769
BspLI GGNNCC 5 cut(s) 21, 282, 429, 604, 852
BspMI ACCTGC 1 cut(s) 649
BspPI GGATC 6 cut(s) 14, 27, 313, 341, 718, 890
BspQI GCTCTTC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 956
BsrBI CCGCTC 1 cut(s) 65
BsrI ACTGG 1 cut(s) 966
BssECI CCNNGG 3 cut(s) 15, 208, 945
BssHII GCGCGC 1 cut(s) 261
BssMI GATC 6 cut(s) 19, 91, 305, 346, 723, 895
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 209, 636
Bst6I CTCTTC 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 263
BstDEI CTNAG 4 cut(s) 471, 753, 960, 993
BstDSI CCRYGG 1 cut(s) 208
BstEII GGTNACC 1 cut(s) 636
BstF5I GGATG 2 cut(s) 324, 787
BstFNI CGCG 2 cut(s) 261, 263
BstHHI GCGC 4 cut(s) 159, 263, 265, 868
BstKTI GATC 6 cut(s) 22, 94, 308, 349, 726, 898
BstMAI GTCTC 4 cut(s) 616, 781, 828, 956
BstMBI GATC 6 cut(s) 19, 91, 305, 346, 723, 895
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 6 cut(s) 48, 54, 57, 71, 77, 83
BstNI CCWGG 1 cut(s) 24
BstPI GGTNACC 1 cut(s) 636
BstSCI CCNGG 1 cut(s) 22
BstUI CGCG 2 cut(s) 261, 263
BstV1I GCAGC 2 cut(s) 58, 63
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 2 cut(s) 19, 305
BstYI RGATCY 2 cut(s) 19, 305
Bsu36I CCTNAGG 1 cut(s) 993
BsuRI GGCC 3 cut(s) 149, 430, 991
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 2 cut(s) 324, 787
BtsI GCAGTG 2 cut(s) 235, 869
BtsIMutI CAGTG 3 cut(s) 235, 869, 973
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 1 cut(s) 263
CciI TCATGA 1 cut(s) 769
CfoI GCGC 4 cut(s) 159, 263, 265, 868
Cfr13I GGNCC 2 cut(s) 26, 428
CviAII CATG 5 cut(s) 457, 485, 619, 770, 889
DdeI CTNAG 4 cut(s) 471, 753, 960, 993
DpnI GATC 6 cut(s) 21, 93, 307, 348, 725, 897
DpnII GATC 6 cut(s) 19, 91, 305, 346, 723, 895
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 200
Eco31I GGTCTC 1 cut(s) 956
Eco47I GGWCC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 993
Eco88I CYCGRG 1 cut(s) 944
Eco91I GGTNACC 1 cut(s) 636
EcoICRI GAGCTC 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 428
EcoO65I GGTNACC 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 22
EcoT22I ATGCAT 1 cut(s) 841
EcoT38I GRGCYC 1 cut(s) 200
FaeI CATG 5 cut(s) 460, 488, 622, 773, 892
FatI CATG 5 cut(s) 456, 484, 618, 769, 888
FblI GTMKAC 1 cut(s) 906
Fnu4HI GCNGC 8 cut(s) 49, 52, 55, 58, 72, 75, 78, 183
FokI GGATG 2 cut(s) 311, 794
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 8 cut(s) 49, 52, 55, 58, 72, 75, 78, 183
FspBI CTAG 1 cut(s) 240
GlaI GCGC 4 cut(s) 158, 262, 264, 867
GluI GCNGC 8 cut(s) 49, 52, 55, 58, 72, 75, 78, 183
GsaI CCCAGC 2 cut(s) 541, 780
GsuI CTGGAG 2 cut(s) 516, 812
HaeIII GGCC 3 cut(s) 149, 430, 991
HhaI GCGC 4 cut(s) 159, 263, 265, 868
Hin1II CATG 5 cut(s) 460, 488, 622, 773, 892
Hin6I GCGC 4 cut(s) 157, 261, 263, 866
HinP1I GCGC 4 cut(s) 157, 261, 263, 866
HinfI GANTC 3 cut(s) 213, 533, 609
HphI GGTGA 2 cut(s) 521, 630
Hpy166II GTNNAC 2 cut(s) 512, 907
Hpy188I TCNGA 5 cut(s) 19, 30, 222, 310, 472
Hpy188III TCNNGA 4 cut(s) 240, 770, 829, 944
Hpy8I GTNNAC 2 cut(s) 512, 907
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 2 cut(s) 209, 636
HpyCH4IV ACGT 2 cut(s) 377, 565
HpyCH4V TGCA 5 cut(s) 144, 384, 692, 839, 971
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 54, 57, 71, 77, 83
HpyF3I CTNAG 4 cut(s) 471, 753, 960, 993
HpySE526I ACGT 2 cut(s) 377, 565
Hsp92II CATG 5 cut(s) 460, 488, 622, 773, 892
HspAI GCGC 4 cut(s) 157, 261, 263, 866
Kzo9I GATC 6 cut(s) 19, 91, 305, 346, 723, 895
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 6 cut(s) 62, 195, 203, 280, 856, 937
Lsp1109I GCAGC 2 cut(s) 58, 63
LweI GCATC 6 cut(s) 168, 333, 549, 633, 677, 763
MaeI CTAG 1 cut(s) 240
MaeII ACGT 2 cut(s) 377, 565
MaeIII GTNAC 5 cut(s) 214, 410, 630, 636, 949
MalI GATC 6 cut(s) 21, 93, 307, 348, 725, 897
MbiI CCGCTC 1 cut(s) 65
MboI GATC 6 cut(s) 19, 91, 305, 346, 723, 895
MboII GAAGA 5 cut(s) 43, 130, 139, 235, 932
MfeI CAATTG 1 cut(s) 98
MflI RGATCY 2 cut(s) 19, 305
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 3 cut(s) 98, 365, 672
MlyI GAGTC 2 cut(s) 222, 618
Mph1103I ATGCAT 1 cut(s) 841
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 3 cut(s) 404, 516, 675
MslI CAYNNNNRTG 1 cut(s) 526
MspA1I CMGCKG 2 cut(s) 54, 185
MspR9I CCNGG 1 cut(s) 24
MunI CAATTG 1 cut(s) 98
Mva1269I GAATGC 4 cut(s) 694, 799, 839, 841
MvaI CCWGG 1 cut(s) 24
MvnI CGCG 2 cut(s) 261, 263
MwoI GCNNNNNNNGC 6 cut(s) 48, 54, 57, 71, 77, 83
NdeII GATC 6 cut(s) 19, 91, 305, 346, 723, 895
NlaIII CATG 5 cut(s) 460, 488, 622, 773, 892
NlaIV GGNNCC 5 cut(s) 21, 282, 429, 604, 852
NmuCI GTSAC 3 cut(s) 214, 630, 636
NsiI ATGCAT 1 cut(s) 841
PagI TCATGA 1 cut(s) 769
PaqCI CACCTGC 1 cut(s) 649
PauI GCGCGC 1 cut(s) 261
PciSI GCTCTTC 1 cut(s) 218
PctI GAATGC 4 cut(s) 694, 799, 839, 841
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 1 cut(s) 533
PflMI CCANNNNNTGG 2 cut(s) 290, 918
PkrI GCNGC 8 cut(s) 50, 53, 56, 59, 73, 76, 79, 184
PleI GAGTC 2 cut(s) 221, 617
PpsI GAGTC 2 cut(s) 221, 617
PsiI TTATAA 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 200
Psp1406I AACGTT 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 22
PspEI GGTNACC 1 cut(s) 636
PspFI CCCAGC 2 cut(s) 537, 776
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 5 cut(s) 21, 282, 429, 604, 852
PspPI GGNCC 2 cut(s) 26, 428
PsuI RGATCY 2 cut(s) 19, 305
PteI GCGCGC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 526
SacI GAGCTC 1 cut(s) 200
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 3 cut(s) 404, 516, 675
SatI GCNGC 8 cut(s) 49, 52, 55, 58, 72, 75, 78, 183
Sau3AI GATC 6 cut(s) 19, 91, 305, 346, 723, 895
Sau96I GGNCC 2 cut(s) 26, 428
SchI GAGTC 2 cut(s) 222, 618
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 200
SetI ASST 9 cut(s) 142, 200, 380, 511, 568, 589, 643, 656, 951
SfaNI GCATC 6 cut(s) 168, 333, 549, 633, 677, 763
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 526
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 3 cut(s) 98, 365, 672
SspMI CTAG 1 cut(s) 240
SstI GAGCTC 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 209, 636
TaiI ACGT 2 cut(s) 380, 568
TaqII GACCGA 2 cut(s) 50, 104
TasI AATT 3 cut(s) 98, 365, 672
TauI GCSGC 6 cut(s) 51, 57, 60, 77, 80, 185
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 3 cut(s) 404, 516, 675
Tru9I TTAA 3 cut(s) 404, 516, 675
TscAI CASTG 3 cut(s) 235, 876, 973
TseFI GTSAC 3 cut(s) 214, 630, 636
TseI GCWGC 2 cut(s) 51, 71
Tsp45I GTSAC 3 cut(s) 214, 630, 636
TspDTI ATGAA 3 cut(s) 378, 723, 789
TspGWI ACGGA 2 cut(s) 208, 843
TspRI CASTG 3 cut(s) 235, 876, 973
Van91I CCANNNNNTGG 2 cut(s) 290, 918
VpaK11BI GGWCC 1 cut(s) 26
XapI RAATTY 1 cut(s) 365
XbaI TCTAGA 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 906
XmnI GAANNNNTTC 1 cut(s) 839
XspI CTAG 1 cut(s) 240
Zsp2I ATGCAT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.