MD14G1076000.v1.1

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006364
Physical Location & Seq
Reverse (-)
8596582 .. 8600049
3468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1076000.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGCGTCGTCGTCCTCGGATCCCGGTCCGAAGACCGAAAGCGGAAGCGGGGGCGGCAGTGGCGGTGGAGGAGTAGAGGCGTCGGAGGCGGTTATAGCGAGCGATCAGCTAATGGTGTACAGAGGGGTGAAGAAATCGAAGAAAGAGAGAGGATGTACAGCGAAAGAGCGCATCAGCAAAATGCCTCCGTGTGCTGCTGGAAAACGGAGCTCCATTTACCGTGGAGTCACTCGGCATAGATGGACAGGTCGTTATGAGGCTCATCTTTGGGATAAAAGTACCTGGAATCACAACCAGAATAAGAAGGGAAAGCAAGGGGCGTATGATGATGAAGAGGCTGCAGCTAGAGCTTATGATCTTGCTGCCTTGAAATACTGGGGTCCTGGCACTCTCATCAATTTTCCTGTTACTGATTACACAAGGGATCTTGAAGAGATGCAGATTGTGTCAAGGGAGGAATACCTTGCTTCTCTTCGAAGAAAGAGCAGTGGTTTCTCAAGAGGAATTTCCAAATTTCGTGGGCTTTCTAGTCGTTGGGAGCCGTCATTGGGTCGTATGGGTGGATCTGAGTACTTCAATAGCACACATTATGGCGTTGATCCAGCAACAGAAAGTGAAATTTTGGGAGCTCTTTGCATTGAAAGAAAGATTGACCTAACGAGTTACATCAAGTGGTGGGGACCCAACAGATCTCGACAAGCTGAAACTAGCATGAAATTTTCAGAAGAAACAAAACATGGTTGTGTTGGAGATTTTGGAGTTGAGCTTAAAACATTAGAATGGGGAATCCAGCCTACTGAACCATACCAGATGCCCCGTTTGGGCATCTCTCGTGTAAGTAAAAAGCATAAAGGTACCAGAGTCTCTGCCATGAGCATCCTCTCACGGTCAGCTGCCTATAAGAACTTGCAAGAGAAAGCATCACAAAAGGAGGAAAACAATGCAGATAATGATGAGAATGAAAACAAAAATATCATCCACAAGATGGACTATGGCAAGGCAATTGAGAAATCCACAAGTCATGACGAAAGACTTAGTTCTGTATTAGGAATGAGTGGTGGAATGTCTCTCCAAACGGACGTGTTCCCGTTGGCTCCCTTCTTATCTGCACCACTTCTGACCAACTACAATGCCGTTGATCCCTTGGTAGACCCCATTCTTTGGACGTCTCTTGCTCCAGTTCTCCCTTCTGGAATTTCTCGCACGACTGAGATTACAAAGACAGAGACAAGTCCAACTTTCACTTTATTTCAGCCTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

422

Amino Acids

46.59

Weight (kDa)

8.77

Isoelectric Point (pI)

56.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 73 - 127 1.9e-07 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1169
Acc65I GGTACC 1 cut(s) 854
AccB1I GGYRCC 1 cut(s) 854
AccB7I CCANNNNNTGG 2 cut(s) 985, 1161
AccI GTMKAC 1 cut(s) 1149
AciI CCGC 5 cut(s) 42, 48, 54, 63, 89
AclWI GGATC 6 cut(s) 14, 27, 432, 571, 593, 1133
AcsI RAATTY 5 cut(s) 504, 512, 618, 716, 1194
AcyI GRCGYC 3 cut(s) 5, 80, 1166
AfaI GTAC 5 cut(s) 119, 157, 280, 572, 856
AfiI CCNNNNNNNGG 4 cut(s) 548, 821, 985, 1161
AflIII ACRYGT 1 cut(s) 1080
AgsI TTSAA 4 cut(s) 370, 431, 577, 641
AjiI CACGTC 1 cut(s) 1081
AjnI CCWGG 2 cut(s) 281, 382
AluBI AGCT 8 cut(s) 109, 210, 344, 350, 629, 701, 766, 893
AluI AGCT 8 cut(s) 109, 210, 344, 350, 629, 701, 766, 893
Alw21I GWGCWC 2 cut(s) 212, 631
Alw26I GTCTC 4 cut(s) 868, 1071, 1173, 1220
AlwI GGATC 6 cut(s) 14, 27, 432, 571, 593, 1133
ApeKI GCWGC 5 cut(s) 194, 338, 341, 362, 893
ApoI RAATTY 5 cut(s) 504, 512, 618, 716, 1194
Asp718I GGTACC 1 cut(s) 854
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 3 cut(s) 26, 380, 680
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 1 cut(s) 139
AsuII TTCGAA 1 cut(s) 475
AvaII GGWCC 3 cut(s) 26, 380, 680
BaeI ACNNNNGTAYC 2 cut(s) 846, 879
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 854
BanII GRGCYC 2 cut(s) 212, 631
BauI CACGAG 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 212, 631
BbvI GCAGC 5 cut(s) 181, 325, 349, 353, 880
BccI CCATC 2 cut(s) 234, 979
BceAI ACGGC 2 cut(s) 526, 1118
BciT130I CCWGG 2 cut(s) 283, 384
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 4 cut(s) 868, 1071, 1173, 1220
BfaI CTAG 3 cut(s) 345, 528, 708
BfmI CTRYAG 1 cut(s) 339
BglII AGATCT 1 cut(s) 689
BisI GCNGC 6 cut(s) 55, 195, 339, 342, 363, 894
BlsI GCNGC 6 cut(s) 56, 196, 340, 343, 364, 895
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 3 cut(s) 24, 283, 384
Bme18I GGWCC 3 cut(s) 26, 380, 680
BmgBI CACGTC 1 cut(s) 1081
BmgT120I GGNCC 3 cut(s) 26, 380, 680
BmiI GGNNCC 7 cut(s) 21, 381, 540, 681, 682, 856, 1095
BmrFI CCNGG 3 cut(s) 24, 283, 384
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 6 cut(s) 180, 426, 801, 834, 885, 929
BmuI ACTGGG 1 cut(s) 385
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 1161
Bpu14I TTCGAA 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 481
BpuMI CCSGG 1 cut(s) 24
BsaHI GRCGYC 3 cut(s) 5, 80, 1166
BsaJI CCNNGG 3 cut(s) 15, 220, 1143
Bsc4I CCNNNNNNNGG 4 cut(s) 548, 821, 985, 1161
Bse1I ACTGG 2 cut(s) 380, 1178
BseBI CCWGG 2 cut(s) 283, 384
BseDI CCNNGG 3 cut(s) 15, 220, 1143
BseGI GGATG 3 cut(s) 158, 876, 975
BseLI CCNNNNNNNGG 4 cut(s) 548, 821, 985, 1161
BseMII CTCAG 2 cut(s) 558, 1200
BseNI ACTGG 2 cut(s) 380, 1178
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 5 cut(s) 181, 325, 349, 353, 880
BsgI GTGCAG 1 cut(s) 1092
BshNI GGYRCC 1 cut(s) 854
BsiHKAI GWGCWC 2 cut(s) 212, 631
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 1 cut(s) 693
BslI CCNNNNNNNGG 4 cut(s) 548, 821, 985, 1161
BsmAI GTCTC 4 cut(s) 868, 1071, 1173, 1220
BsmBI CGTCTC 1 cut(s) 1173
BsmFI GGGAC 1 cut(s) 693
Bsp119I TTCGAA 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 212, 631
Bsp1407I TGTACA 2 cut(s) 117, 155
Bsp143I GATC 8 cut(s) 19, 103, 355, 424, 563, 598, 689, 1138
BspACI CCGC 5 cut(s) 42, 48, 54, 63, 89
BspCNI CTCAG 2 cut(s) 559, 1201
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 7 cut(s) 21, 381, 540, 681, 682, 856, 1095
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 6 cut(s) 14, 27, 432, 571, 593, 1133
BspT104I TTCGAA 1 cut(s) 475
BspT107I GGYRCC 1 cut(s) 854
BsrGI TGTACA 2 cut(s) 117, 155
BsrI ACTGG 2 cut(s) 380, 1178
BssECI CCNNGG 3 cut(s) 15, 220, 1143
BssMI GATC 8 cut(s) 19, 103, 355, 424, 563, 598, 689, 1138
BssNI GRCGYC 3 cut(s) 5, 80, 1166
BssSI CACGAG 1 cut(s) 831
BssT1I CCWWGG 1 cut(s) 1143
Bst2BI CACGAG 1 cut(s) 831
Bst2UI CCWGG 2 cut(s) 283, 384
Bst4CI ACNGT 2 cut(s) 221, 888
Bst6I CTCTTC 3 cut(s) 327, 426, 477
BstACI GRCGYC 3 cut(s) 5, 80, 1166
BstAUI TGTACA 2 cut(s) 117, 155
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 3 cut(s) 567, 1034, 1209
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 3 cut(s) 158, 876, 975
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 8 cut(s) 22, 106, 358, 427, 566, 601, 692, 1141
BstMAI GTCTC 4 cut(s) 868, 1071, 1173, 1220
BstMBI GATC 8 cut(s) 19, 103, 355, 424, 563, 598, 689, 1138
BstMWI GCNNNNNNNGC 5 cut(s) 54, 60, 86, 95, 347
BstNI CCWGG 2 cut(s) 283, 384
BstSCI CCNGG 3 cut(s) 22, 281, 382
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 5 cut(s) 181, 325, 349, 353, 880
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 4 cut(s) 19, 424, 563, 689
BstYI RGATCY 4 cut(s) 19, 424, 563, 689
BtgI CCRYGG 1 cut(s) 220
BtrI CACGTC 1 cut(s) 1081
BtsCI GGATG 3 cut(s) 158, 876, 975
BtsI GCAGTG 2 cut(s) 64, 493
BtsIMutI CAGTG 2 cut(s) 64, 493
Cac8I GCNNGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 3 cut(s) 26, 380, 680
CpoI CGGWCCG 1 cut(s) 26
CseI GACGC 1 cut(s) 69
Csp6I GTAC 5 cut(s) 118, 156, 279, 571, 855
CspCI CAANNNNNGTGG 2 cut(s) 499, 534
CspI CGGWCCG 1 cut(s) 26
CviAII CATG 4 cut(s) 712, 737, 871, 1022
CviQI GTAC 5 cut(s) 118, 156, 279, 571, 855
DdeI CTNAG 3 cut(s) 567, 1034, 1209
DpnI GATC 8 cut(s) 21, 105, 357, 426, 565, 600, 691, 1140
DpnII GATC 8 cut(s) 19, 103, 355, 424, 563, 598, 689, 1138
Eam1104I CTCTTC 3 cut(s) 327, 426, 477
EarI CTCTTC 3 cut(s) 327, 426, 477
Ecl136II GAGCTC 2 cut(s) 210, 629
Eco130I CCWWGG 1 cut(s) 1143
Eco24I GRGCYC 2 cut(s) 212, 631
Eco47I GGWCC 3 cut(s) 26, 380, 680
Eco53kI GAGCTC 2 cut(s) 210, 629
EcoICRI GAGCTC 2 cut(s) 210, 629
EcoO109I RGGNCCY 2 cut(s) 380, 680
EcoRII CCWGG 2 cut(s) 281, 382
EcoT14I CCWWGG 1 cut(s) 1143
EcoT38I GRGCYC 2 cut(s) 212, 631
ErhI CCWWGG 1 cut(s) 1143
Esp3I CGTCTC 1 cut(s) 1173
FaeI CATG 4 cut(s) 715, 740, 874, 1025
FalI AAGNNNNNCTT 2 cut(s) 1222, 1254
FaqI GGGAC 1 cut(s) 693
FatI CATG 4 cut(s) 711, 736, 870, 1021
FauI CCCGC 1 cut(s) 41
FblI GTMKAC 1 cut(s) 1149
Fnu4HI GCNGC 6 cut(s) 55, 195, 339, 342, 363, 894
FokI GGATG 3 cut(s) 165, 863, 962
FriOI GRGCYC 2 cut(s) 212, 631
Fsp4HI GCNGC 6 cut(s) 55, 195, 339, 342, 363, 894
FspBI CTAG 3 cut(s) 345, 528, 708
GlaI GCGC 1 cut(s) 170
GluI GCNGC 6 cut(s) 55, 195, 339, 342, 363, 894
GsuI CTGGAG 1 cut(s) 1161
HapII CCGG 1 cut(s) 24
HgaI GACGC 1 cut(s) 69
HhaI GCGC 1 cut(s) 171
Hin1I GRCGYC 3 cut(s) 5, 80, 1166
Hin1II CATG 4 cut(s) 715, 740, 874, 1025
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 4 cut(s) 225, 286, 786, 861
HpaII CCGG 1 cut(s) 24
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 2 cut(s) 118, 1150
Hpy188I TCNGA 6 cut(s) 19, 30, 85, 568, 724, 1119
Hpy188III TCNNGA 5 cut(s) 428, 498, 693, 1022, 1191
Hpy8I GTNNAC 2 cut(s) 118, 1150
Hpy99I CGWCG 3 cut(s) 10, 13, 85
HpyAV CCTTC 3 cut(s) 298, 1108, 1197
HpyCH4III ACNGT 2 cut(s) 221, 888
HpyCH4IV ACGT 2 cut(s) 1080, 1166
HpyCH4V TGCA 6 cut(s) 341, 439, 636, 910, 944, 1109
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 60, 86, 95, 347
HpyF3I CTNAG 3 cut(s) 567, 1034, 1209
HpySE526I ACGT 2 cut(s) 1080, 1166
Hsp92I GRCGYC 3 cut(s) 5, 80, 1166
Hsp92II CATG 4 cut(s) 715, 740, 874, 1025
HspAI GCGC 1 cut(s) 169
KflI GGGWCCC 1 cut(s) 680
KpnI GGTACC 1 cut(s) 858
Kzo9I GATC 8 cut(s) 19, 103, 355, 424, 563, 598, 689, 1138
LmnI GCTCC 6 cut(s) 207, 215, 538, 626, 1099, 1180
Lsp1109I GCAGC 5 cut(s) 181, 325, 349, 353, 880
LweI GCATC 6 cut(s) 180, 426, 801, 834, 885, 929
MaeI CTAG 3 cut(s) 345, 528, 708
MaeII ACGT 2 cut(s) 1080, 1166
MaeIII GTNAC 3 cut(s) 226, 406, 662
MalI GATC 8 cut(s) 21, 105, 357, 426, 565, 600, 691, 1140
MboI GATC 8 cut(s) 19, 103, 355, 424, 563, 598, 689, 1138
MboII GAAGA 8 cut(s) 43, 142, 151, 344, 443, 464, 489, 737
MfeI CAATTG 1 cut(s) 1002
MflI RGATCY 4 cut(s) 19, 424, 563, 689
MhlI GDGCHC 2 cut(s) 212, 631
MluCI AATT 7 cut(s) 397, 504, 512, 618, 716, 1002, 1194
MlyI GAGTC 2 cut(s) 234, 870
MmeI TCCRAC 3 cut(s) 63, 727, 1259
MseI TTAA 1 cut(s) 768
MslI CAYNNNNRTG 2 cut(s) 741, 778
MspA1I CMGCKG 1 cut(s) 893
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 3 cut(s) 24, 283, 384
MunI CAATTG 1 cut(s) 1002
MvaI CCWGG 2 cut(s) 283, 384
MwoI GCNNNNNNNGC 5 cut(s) 54, 60, 86, 95, 347
NciI CCSGG 1 cut(s) 24
NdeII GATC 8 cut(s) 19, 103, 355, 424, 563, 598, 689, 1138
NlaIII CATG 4 cut(s) 715, 740, 874, 1025
NlaIV GGNNCC 7 cut(s) 21, 381, 540, 681, 682, 856, 1095
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 226
NspV TTCGAA 1 cut(s) 475
PagI TCATGA 1 cut(s) 1021
PfeI GAWTC 2 cut(s) 286, 786
PflMI CCANNNNNTGG 2 cut(s) 985, 1161
PkrI GCNGC 6 cut(s) 56, 196, 340, 343, 364, 895
PleI GAGTC 2 cut(s) 233, 869
PpsI GAGTC 2 cut(s) 233, 869
PpuMI RGGWCCY 2 cut(s) 380, 680
Psp124BI GAGCTC 2 cut(s) 212, 631
Psp5II RGGWCCY 2 cut(s) 380, 680
Psp6I CCWGG 2 cut(s) 281, 382
PspGI CCWGG 2 cut(s) 281, 382
PspN4I GGNNCC 7 cut(s) 21, 381, 540, 681, 682, 856, 1095
PspPI GGNCC 3 cut(s) 26, 380, 680
PspPPI RGGWCCY 2 cut(s) 380, 680
PstI CTGCAG 1 cut(s) 343
PsuI RGATCY 4 cut(s) 19, 424, 563, 689
PvuII CAGCTG 1 cut(s) 893
RsaI GTAC 5 cut(s) 119, 157, 280, 572, 856
RsaNI GTAC 5 cut(s) 118, 156, 279, 571, 855
RseI CAYNNNNRTG 2 cut(s) 741, 778
Rsr2I CGGWCCG 1 cut(s) 26
RsrII CGGWCCG 1 cut(s) 26
SacI GAGCTC 2 cut(s) 212, 631
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 6 cut(s) 55, 195, 339, 342, 363, 894
Sau3AI GATC 8 cut(s) 19, 103, 355, 424, 563, 598, 689, 1138
Sau96I GGNCC 3 cut(s) 26, 380, 680
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 2 cut(s) 234, 870
ScrFI CCNGG 3 cut(s) 24, 283, 384
SduI GDGCHC 2 cut(s) 212, 631
SfaNI GCATC 6 cut(s) 180, 426, 801, 834, 885, 929
SfcI CTRYAG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 475
SinI GGWCC 3 cut(s) 26, 380, 680
SmiMI CAYNNNNRTG 2 cut(s) 741, 778
SmlI CTYRAG 1 cut(s) 496
SmoI CTYRAG 1 cut(s) 496
Sse9I AATT 7 cut(s) 397, 504, 512, 618, 716, 1002, 1194
SsiI CCGC 5 cut(s) 42, 48, 54, 63, 89
SspMI CTAG 3 cut(s) 345, 528, 708
SstI GAGCTC 2 cut(s) 212, 631
StyD4I CCNGG 3 cut(s) 22, 281, 382
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 2 cut(s) 221, 888
TaiI ACGT 2 cut(s) 1083, 1169
TaqI TCGA 3 cut(s) 137, 475, 694
TaqII GACCGA 1 cut(s) 50
TasI AATT 7 cut(s) 397, 504, 512, 618, 716, 1002, 1194
TatI WGTACW 3 cut(s) 117, 155, 570
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 2 cut(s) 286, 786
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 2 cut(s) 64, 493
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 5 cut(s) 194, 338, 341, 362, 893
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 3 cut(s) 345, 728, 975
TspGWI ACGGA 3 cut(s) 177, 220, 1091
TspRI CASTG 2 cut(s) 64, 493
Van91I CCANNNNNTGG 2 cut(s) 985, 1161
VpaK11BI GGWCC 3 cut(s) 26, 380, 680
XapI RAATTY 5 cut(s) 504, 512, 618, 716, 1194
XmiI GTMKAC 1 cut(s) 1149
XspI CTAG 3 cut(s) 345, 528, 708
ZraI GACGTC 1 cut(s) 1167
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.