Rw3G022500

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0006364
Physical Location & Seq
Forward (+)
27592554 .. 27596266
3713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022500.1

Sequence Viewer

Length: 1257 bp
ATGGCTTCGTCGTCCTCGGATCCTGGTCCGAAGACCGAAAGCGGAAGCGGCAGCGGCGGCGGAGCGGAGGCTGCGGCGGCGGTGGCGACCGATCAGCAATTGCTTTACAGAGGGCTGAAGAAAGCGAAGAAAGAGAGAGGTTGCACGGCCAAAGAGCGCATCAGCAAAATGCCTCCCTGTGCCGCTGGAAAACGCAGCTCCATTTACCGTGGAGTCACTCGGCATAGGTGGACGGGTCGTTACGAAGCTCATCTCTGGGATAAAAGTACCTGGAACCAGAACCAGAATAAGAAGGGAAAGCAAGTTTACTTGGGCGCGTATGATGATGAAGAGGCTGCAGCCAGAGCTTACGATCTTGCTGCCTTAAAATATTGGGGCCCTGGCACTCTGATTAATTTCCCTGTTACTGACTACACAAGAGATCTCGAAGAGATGCAGAATGTGTCAAGGGAGGAATATCTTGCATCTCTTCGCAGGAAGAGCAGTGGCTTCTCTAGAGGAATGTCTAAATATCGCGCGCTCTCAAGCCGTTGGGAGCCATCATTTGGTCGTATGGGTGGATCTGAATACTTCAATAGCATCCATTACGGCACAGGAGATGATCCAGCAACAGACAATGAATTTTTAGGAACGTTTTGCATTGAAAGAAAGATTGATTTAACAAGTTACATCAAGTGGTGGGGGCCTAACAAAACTCGTCAAGCGGACAGCATGATAAAATCATCTGAGGAAGCAAAACATGGTTTTGCTGGAGATAGTGGAGGTGAACTTAAAACATTGGAATGGGAATCCCAGCCTACTGAACCATACCAGATGCCACGTTTGGGCGTCTGCCGTGAAGGTAAAAAGCAAAATGGTGCCAGAGTCTCTGCCATGAGCATCTTGTCACGGTCACCTGCTTATAAGAGCTTACAAGAGAAAGCATCAATTAAGCAAGAAAAGAATGCAGATAATGACGAGAATGAAAACAAAAATACGATCCACAAAAAGGACTATGGCAAGGCAATTGAGAAATCCACAAGTCATGATGCTGGGGATGAGAGACTGAATGCTTCATTAGGAATGAGTGGTGGATTGTCTCTCCAGAGGAATGCATTCCCATTGGCTCCGTTCTTGTCTGCGCCACTGCTGACCAACTACAACATGATTGATCCATTAGTAGACCCCATTCTTTGGACATCTCTTGCTCCTGTTCTTCCCGAGGTTACAAAGACTGAGACCAGTGCAAGTTTCAGTTTATTTCGGCCTGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

418

Amino Acids

46.34

Weight (kDa)

8.85

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 69 - 126 2.6e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AarI CACCTGC 1 cut(s) 904
Acc36I ACCTGC 1 cut(s) 904
AccB1I GGYRCC 1 cut(s) 857
AccB7I CCANNNNNTGG 2 cut(s) 545, 1173
AccBSI CCGCTC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 1161
AccII CGCG 3 cut(s) 317, 516, 518
AclI AACGTT 1 cut(s) 632
AclWI GGATC 6 cut(s) 14, 27, 568, 596, 973, 1145
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 828
AfaI GTAC 1 cut(s) 268
AfiI CCNNNNNNNGG 4 cut(s) 545, 824, 988, 1173
AgsI TTSAA 2 cut(s) 574, 644
AjnI CCWGG 3 cut(s) 22, 269, 379
AluBI AGCT 4 cut(s) 198, 248, 347, 909
AluI AGCT 4 cut(s) 198, 248, 347, 909
Alw26I GTCTC 4 cut(s) 871, 1036, 1083, 1211
AlwI GGATC 6 cut(s) 14, 27, 568, 596, 973, 1145
Ama87I CYCGRG 1 cut(s) 1199
AoxI GGCC 4 cut(s) 147, 376, 683, 1244
ApaI GGGCCC 1 cut(s) 380
ApeKI GCWGC 6 cut(s) 51, 71, 195, 335, 338, 359
ApoI RAATTY 1 cut(s) 620
AseI ATTAAT 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 1094
AspLEI GCGC 5 cut(s) 159, 317, 518, 520, 1123
AspS9I GGNCC 4 cut(s) 26, 376, 377, 683
AsuHPI GGTGA 2 cut(s) 776, 885
AvaI CYCGRG 1 cut(s) 1199
AvaII GGWCC 1 cut(s) 26
AxyI CCTNAGG 1 cut(s) 1248
BaeGI GKGCMC 1 cut(s) 380
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 6 cut(s) 58, 63, 207, 322, 346, 350
BccI CCATC 1 cut(s) 547
BceAI ACGGC 4 cut(s) 162, 513, 604, 819
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 3 cut(s) 24, 271, 381
BcoDI GTCTC 4 cut(s) 871, 1036, 1083, 1211
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 904
BglII AGATCT 1 cut(s) 421
Bme1390I CCNGG 3 cut(s) 24, 271, 381
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 1199
BmgT120I GGNCC 4 cut(s) 26, 376, 377, 683
BmiI GGNNCC 8 cut(s) 21, 275, 377, 378, 537, 684, 859, 1107
BmrFI CCNGG 3 cut(s) 24, 271, 381
BmsI GCATC 8 cut(s) 168, 423, 473, 588, 804, 888, 932, 1018
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 2 cut(s) 771, 1067
BpuEI CTTGAG 1 cut(s) 508
BsaHI GRCGYC 1 cut(s) 828
BsaI GGTCTC 1 cut(s) 1211
BsaJI CCNNGG 4 cut(s) 15, 208, 379, 1200
Bsc4I CCNNNNNNNGG 4 cut(s) 545, 824, 988, 1173
Bse1I ACTGG 1 cut(s) 1221
Bse21I CCTNAGG 1 cut(s) 1248
BseBI CCWGG 3 cut(s) 24, 271, 381
BseDI CCNNGG 4 cut(s) 15, 208, 379, 1200
BseGI GGATG 2 cut(s) 579, 1042
BseLI CCNNNNNNNGG 4 cut(s) 545, 824, 988, 1173
BseMII CTCAG 3 cut(s) 717, 1206, 1239
BseNI ACTGG 1 cut(s) 1221
BsePI GCGCGC 1 cut(s) 516
BseSI GKGCMC 1 cut(s) 380
BseXI GCAGC 6 cut(s) 58, 63, 207, 322, 346, 350
BseYI CCCAGC 2 cut(s) 792, 1031
Bsh1236I CGCG 3 cut(s) 317, 516, 518
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 4 cut(s) 149, 378, 685, 1246
BshNI GGYRCC 1 cut(s) 857
BsiEI CGRYCG 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 1199
BslI CCNNNNNNNGG 4 cut(s) 545, 824, 988, 1173
BsmAI GTCTC 4 cut(s) 871, 1036, 1083, 1211
BsmI GAATGC 4 cut(s) 949, 1054, 1094, 1096
BsnI GGCC 4 cut(s) 149, 378, 685, 1246
Bso31I GGTCTC 1 cut(s) 1211
BsoBI CYCGRG 1 cut(s) 1199
Bsp120I GGGCCC 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 380
Bsp143I GATC 8 cut(s) 19, 91, 352, 421, 560, 601, 978, 1150
BspANI GGCC 4 cut(s) 149, 378, 685, 1246
BspCNI CTCAG 3 cut(s) 718, 1207, 1240
BspFNI CGCG 3 cut(s) 317, 516, 518
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 8 cut(s) 21, 275, 377, 378, 537, 684, 859, 1107
BspMAI CTGCAG 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 904
BspPI GGATC 6 cut(s) 14, 27, 568, 596, 973, 1145
BspQI GCTCTTC 1 cut(s) 473
BspT107I GGYRCC 1 cut(s) 857
BspTNI GGTCTC 1 cut(s) 1211
BsrBI CCGCTC 1 cut(s) 65
BsrI ACTGG 1 cut(s) 1221
BssECI CCNNGG 4 cut(s) 15, 208, 379, 1200
BssHII GCGCGC 1 cut(s) 516
BssMI GATC 8 cut(s) 19, 91, 352, 421, 560, 601, 978, 1150
BssNI GRCGYC 1 cut(s) 828
Bst2UI CCWGG 3 cut(s) 24, 271, 381
Bst4CI ACNGT 2 cut(s) 209, 891
Bst6I CTCTTC 4 cut(s) 324, 423, 473, 474
BstACI GRCGYC 1 cut(s) 828
BstC8I GCNNGC 1 cut(s) 518
BstDEI CTNAG 3 cut(s) 726, 1215, 1248
BstDSI CCRYGG 1 cut(s) 208
BstEII GGTNACC 1 cut(s) 891
BstF5I GGATG 2 cut(s) 579, 1042
BstFNI CGCG 3 cut(s) 317, 516, 518
BstHHI GCGC 5 cut(s) 159, 317, 518, 520, 1123
BstKTI GATC 8 cut(s) 22, 94, 355, 424, 563, 604, 981, 1153
BstMAI GTCTC 4 cut(s) 871, 1036, 1083, 1211
BstMBI GATC 8 cut(s) 19, 91, 352, 421, 560, 601, 978, 1150
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 8 cut(s) 48, 54, 57, 71, 77, 83, 344, 480
BstNI CCWGG 3 cut(s) 24, 271, 381
BstPI GGTNACC 1 cut(s) 891
BstSCI CCNGG 3 cut(s) 22, 269, 379
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 380
BstUI CGCG 3 cut(s) 317, 516, 518
BstV1I GCAGC 6 cut(s) 58, 63, 207, 322, 346, 350
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 3 cut(s) 19, 421, 560
BstYI RGATCY 3 cut(s) 19, 421, 560
Bsu36I CCTNAGG 1 cut(s) 1248
BsuRI GGCC 4 cut(s) 149, 378, 685, 1246
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 2 cut(s) 579, 1042
BtsI GCAGTG 2 cut(s) 490, 1124
BtsIMutI CAGTG 3 cut(s) 490, 1124, 1228
BveI ACCTGC 1 cut(s) 904
Cac8I GCNNGC 1 cut(s) 518
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 5 cut(s) 159, 317, 518, 520, 1123
Cfr13I GGNCC 4 cut(s) 26, 376, 377, 683
CseI GACGC 1 cut(s) 817
Csp6I GTAC 1 cut(s) 267
CviAII CATG 5 cut(s) 712, 740, 874, 1025, 1144
CviQI GTAC 1 cut(s) 267
DdeI CTNAG 3 cut(s) 726, 1215, 1248
DpnI GATC 8 cut(s) 21, 93, 354, 423, 562, 603, 980, 1152
DpnII GATC 8 cut(s) 19, 91, 352, 421, 560, 601, 978, 1150
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 4 cut(s) 324, 423, 473, 474
EarI CTCTTC 4 cut(s) 324, 423, 473, 474
EciI GGCGGA 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 380
Eco31I GGTCTC 1 cut(s) 1211
Eco47I GGWCC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 1 cut(s) 1199
Eco91I GGTNACC 1 cut(s) 891
EcoO109I RGGNCCY 3 cut(s) 376, 377, 683
EcoO65I GGTNACC 1 cut(s) 891
EcoRII CCWGG 3 cut(s) 22, 269, 379
EcoT22I ATGCAT 1 cut(s) 1096
EcoT38I GRGCYC 1 cut(s) 380
FaeI CATG 5 cut(s) 715, 743, 877, 1028, 1147
FatI CATG 5 cut(s) 711, 739, 873, 1024, 1143
FblI GTMKAC 1 cut(s) 1161
FokI GGATG 2 cut(s) 566, 1049
FriOI GRGCYC 1 cut(s) 380
FspBI CTAG 1 cut(s) 495
GlaI GCGC 5 cut(s) 158, 316, 517, 519, 1122
GsaI CCCAGC 2 cut(s) 796, 1035
GsuI CTGGAG 2 cut(s) 771, 1067
HaeIII GGCC 4 cut(s) 149, 378, 685, 1246
HgaI GACGC 1 cut(s) 817
HhaI GCGC 5 cut(s) 159, 317, 518, 520, 1123
Hin1I GRCGYC 1 cut(s) 828
Hin1II CATG 5 cut(s) 715, 743, 877, 1028, 1147
Hin6I GCGC 5 cut(s) 157, 315, 516, 518, 1121
HinP1I GCGC 5 cut(s) 157, 315, 516, 518, 1121
HinfI GANTC 3 cut(s) 213, 788, 864
HphI GGTGA 2 cut(s) 776, 885
Hpy166II GTNNAC 4 cut(s) 231, 307, 767, 1162
Hpy188I TCNGA 5 cut(s) 19, 30, 390, 565, 727
Hpy188III TCNNGA 5 cut(s) 425, 495, 1025, 1084, 1199
Hpy8I GTNNAC 4 cut(s) 231, 307, 767, 1162
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 286, 833
HpyCH4III ACNGT 2 cut(s) 209, 891
HpyCH4IV ACGT 2 cut(s) 632, 820
HpyCH4V TGCA 8 cut(s) 144, 338, 436, 464, 639, 947, 1094, 1226
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 54, 57, 71, 77, 83, 344, 480
HpyF3I CTNAG 3 cut(s) 726, 1215, 1248
HpySE526I ACGT 2 cut(s) 632, 820
Hsp92I GRCGYC 1 cut(s) 828
Hsp92II CATG 5 cut(s) 715, 743, 877, 1028, 1147
HspAI GCGC 5 cut(s) 157, 315, 516, 518, 1121
Kzo9I GATC 8 cut(s) 19, 91, 352, 421, 560, 601, 978, 1150
LguI GCTCTTC 1 cut(s) 473
LmnI GCTCC 5 cut(s) 62, 203, 535, 1111, 1192
Lsp1109I GCAGC 6 cut(s) 58, 63, 207, 322, 346, 350
LweI GCATC 8 cut(s) 168, 423, 473, 588, 804, 888, 932, 1018
MaeI CTAG 1 cut(s) 495
MaeII ACGT 2 cut(s) 632, 820
MaeIII GTNAC 7 cut(s) 214, 239, 403, 665, 885, 891, 1204
MalI GATC 8 cut(s) 21, 93, 354, 423, 562, 603, 980, 1152
MbiI CCGCTC 1 cut(s) 65
MboI GATC 8 cut(s) 19, 91, 352, 421, 560, 601, 978, 1150
MboII GAAGA 8 cut(s) 43, 130, 139, 341, 440, 461, 490, 1187
MfeI CAATTG 2 cut(s) 98, 1005
MflI RGATCY 3 cut(s) 19, 421, 560
MhlI GDGCHC 1 cut(s) 380
MluCI AATT 5 cut(s) 98, 394, 620, 927, 1005
MlyI GAGTC 2 cut(s) 222, 873
Mph1103I ATGCAT 1 cut(s) 1096
MroXI GAANNNNTTC 1 cut(s) 1094
MseI TTAA 5 cut(s) 365, 393, 659, 771, 930
MslI CAYNNNNRTG 1 cut(s) 781
MspA1I CMGCKG 2 cut(s) 54, 185
MspR9I CCNGG 3 cut(s) 24, 271, 381
MunI CAATTG 2 cut(s) 98, 1005
Mva1269I GAATGC 4 cut(s) 949, 1054, 1094, 1096
MvaI CCWGG 3 cut(s) 24, 271, 381
MvnI CGCG 3 cut(s) 317, 516, 518
MwoI GCNNNNNNNGC 8 cut(s) 48, 54, 57, 71, 77, 83, 344, 480
NdeII GATC 8 cut(s) 19, 91, 352, 421, 560, 601, 978, 1150
NlaIII CATG 5 cut(s) 715, 743, 877, 1028, 1147
NlaIV GGNNCC 8 cut(s) 21, 275, 377, 378, 537, 684, 859, 1107
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 3 cut(s) 214, 885, 891
NsiI ATGCAT 1 cut(s) 1096
PagI TCATGA 1 cut(s) 1024
PaqCI CACCTGC 1 cut(s) 904
PauI GCGCGC 1 cut(s) 516
PciSI GCTCTTC 1 cut(s) 473
PctI GAATGC 4 cut(s) 949, 1054, 1094, 1096
PdmI GAANNNNTTC 1 cut(s) 1094
PfeI GAWTC 1 cut(s) 788
PflMI CCANNNNNTGG 2 cut(s) 545, 1173
PleI GAGTC 2 cut(s) 221, 872
PpsI GAGTC 2 cut(s) 221, 872
PshBI ATTAAT 1 cut(s) 393
PsiI TTATAA 1 cut(s) 903
Psp1406I AACGTT 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 22, 269, 379
PspEI GGTNACC 1 cut(s) 891
PspFI CCCAGC 2 cut(s) 792, 1031
PspGI CCWGG 3 cut(s) 22, 269, 379
PspN4I GGNNCC 8 cut(s) 21, 275, 377, 378, 537, 684, 859, 1107
PspOMI GGGCCC 1 cut(s) 376
PspPI GGNCC 4 cut(s) 26, 376, 377, 683
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 3 cut(s) 19, 421, 560
PteI GCGCGC 1 cut(s) 516
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
RseI CAYNNNNRTG 1 cut(s) 781
SapI GCTCTTC 1 cut(s) 473
SaqAI TTAA 5 cut(s) 365, 393, 659, 771, 930
Sau3AI GATC 8 cut(s) 19, 91, 352, 421, 560, 601, 978, 1150
Sau96I GGNCC 4 cut(s) 26, 376, 377, 683
SchI GAGTC 2 cut(s) 222, 873
ScrFI CCNGG 3 cut(s) 24, 271, 381
SduI GDGCHC 1 cut(s) 380
SfaNI GCATC 8 cut(s) 168, 423, 473, 588, 804, 888, 932, 1018
SfcI CTRYAG 1 cut(s) 336
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 781
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
Sse9I AATT 5 cut(s) 98, 394, 620, 927, 1005
SspI AATATT 1 cut(s) 371
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 3 cut(s) 22, 269, 379
TaaI ACNGT 2 cut(s) 209, 891
TaiI ACGT 2 cut(s) 635, 823
TaqI TCGA 1 cut(s) 426
TaqII GACCGA 2 cut(s) 50, 104
TasI AATT 5 cut(s) 98, 394, 620, 927, 1005
TauI GCSGC 6 cut(s) 51, 57, 60, 77, 80, 185
TfiI GAWTC 1 cut(s) 788
Tru1I TTAA 5 cut(s) 365, 393, 659, 771, 930
Tru9I TTAA 5 cut(s) 365, 393, 659, 771, 930
TscAI CASTG 3 cut(s) 490, 1131, 1228
TseFI GTSAC 3 cut(s) 214, 885, 891
TseI GCWGC 6 cut(s) 51, 71, 195, 335, 338, 359
Tsp45I GTSAC 3 cut(s) 214, 885, 891
TspDTI ATGAA 4 cut(s) 342, 633, 978, 1044
TspGWI ACGGA 1 cut(s) 1098
TspRI CASTG 3 cut(s) 490, 1131, 1228
Van91I CCANNNNNTGG 2 cut(s) 545, 1173
VpaK11BI GGWCC 1 cut(s) 26
VspI ATTAAT 1 cut(s) 393
XapI RAATTY 1 cut(s) 620
XbaI TCTAGA 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 1161
XmnI GAANNNNTTC 1 cut(s) 1094
XspI CTAG 1 cut(s) 495
Zsp2I ATGCAT 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.