Rroxscaffold_6G00399050

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006364
Physical Location & Seq
Reverse (-)
20950637 .. 20955298
4662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399050.1

Sequence Viewer

Length: 1272 bp
ATGGCTTCGTCGTCCTCGGATCCTGGTCCGAAGACCGAAAGCGGAAGCGGCAGCGGCGGCGGAGCCGAGGCCTCGGAAGCAGCGGCGGCGGCGGCGGCGGCGGCGGTGGCGACCGATCAGCAATTGCTTTACAGAGGGCTGAAGAAAGCCAAGAAAGAGAGAGGTTGCACGGCCAAAGAGCGCATCAGCAAAATGCCTCCCTGTGCCGCTGGAAAACGCAGCTCCATTTACCGCGGAGTCACTCGGCATAGGTGGACGGGTCGTTACGAAGCTCATCTCTGGGATAAAAGTACCTGGAACCAGAACCAGAATAAGAAGGGAAAGCAAGGCGCGTATGATGATGAAGAGGCTGCAGCCAGAGCTTATGATCTTGCTGCCTTGAAATATTGGGGCCCTGGCACTCTGATTAATTTCCCTGTTACTGACTACACAAGAGATCTCGAAGAGATGCAGAATGTGTCAAGGGAGGAATATCTTGCATCTCTTCGCAGGAAGAGCAGTGGCTTCTCTAGAGGAATGTCTAAATATCGCGCGCTCTCAAGCCGTTGGGAGCCATCGTTTGGTCGTATGGGTGGATCTGAATACTTCAATAGCATCCATTATGGCTCAGGAGATGATCCGGCAACAGACAATGAATTTTTAGGAACGTTTTGCATTGAAAGAAAGATTGATTTAACAAGTTACATCAAGTGGTGGGGGCCTAACAAAACTCGTCAAGCGGACAGCACGATAAAATCATCTGAGGAAGCAAAACATGGTTTTGCTGGAGATAGTGGAGGTGAACTTAAAACATTGGAATGGGAATCCCAGCCTTCTGAACCATACCAGATGCCACGTTTGGGTGTCTGCCGTGAAGGTAAAAAGCATAATGGTGCCAGAGTCTCTGCCATGAGCATCTTGTCACGGTCACCTGCTTATAAGAGCTTACAAGAGAAAGCATCAATTAAGCAAGAAAAGAATGCAGATAATGACGAGAATGAAAACAAAAATACGATCCACAAAAAGGACTATGGCAAGGCAATTGAGAAATCCACAAGTCATGATGCTGGGGATGAGAGACTGAATGCTTCATTAGGAATGAGTGGTGGATTGTCTCTCCAGAGGAATGCATTCCCATTGACTCCGTTCTTGTCTGCGCCACTGCTGACCAACTACAACATGATTGATCCATTAGTAGACCCCATTCTTTGGACATCTCTTGCTCCTGTTCTTCCCGAGGTTACAAAGACTGAGACCAGTGCAAGTTTCAGTTTATTTCGGCCTGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

423

Amino Acids

46.59

Weight (kDa)

8.75

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 77 - 131 9.3e-08 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 918
AarI CACCTGC 1 cut(s) 919
Acc36I ACCTGC 1 cut(s) 919
AccB1I GGYRCC 1 cut(s) 872
AccB7I CCANNNNNTGG 2 cut(s) 560, 1188
AccI GTMKAC 1 cut(s) 1176
AccII CGCG 4 cut(s) 234, 332, 531, 533
AclI AACGTT 1 cut(s) 647
AclWI GGATC 6 cut(s) 14, 27, 583, 611, 988, 1160
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 635
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 4 cut(s) 560, 839, 1003, 1188
AgsI TTSAA 3 cut(s) 382, 589, 659
AjnI CCWGG 3 cut(s) 22, 293, 394
AluBI AGCT 4 cut(s) 222, 272, 362, 924
AluI AGCT 4 cut(s) 222, 272, 362, 924
Alw26I GTCTC 4 cut(s) 886, 1051, 1098, 1226
AlwI GGATC 6 cut(s) 14, 27, 583, 611, 988, 1160
Ama87I CYCGRG 1 cut(s) 1214
AoxI GGCC 5 cut(s) 69, 171, 391, 698, 1259
ApaI GGGCCC 1 cut(s) 395
ApeKI GCWGC 6 cut(s) 51, 80, 219, 350, 353, 374
ApoI RAATTY 1 cut(s) 635
AseI ATTAAT 1 cut(s) 408
Asp700I GAANNNNTTC 1 cut(s) 1109
AspLEI GCGC 5 cut(s) 183, 332, 533, 535, 1138
AspS9I GGNCC 4 cut(s) 26, 391, 392, 698
AsuHPI GGTGA 2 cut(s) 791, 900
AvaI CYCGRG 1 cut(s) 1214
AvaII GGWCC 1 cut(s) 26
AxyI CCTNAGG 1 cut(s) 1263
BaeGI GKGCMC 1 cut(s) 395
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 872
BanII GRGCYC 1 cut(s) 395
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 6 cut(s) 63, 92, 231, 337, 361, 365
BccI CCATC 1 cut(s) 562
BceAI ACGGC 3 cut(s) 186, 528, 834
BciT130I CCWGG 3 cut(s) 24, 295, 396
BcoDI GTCTC 4 cut(s) 886, 1051, 1098, 1226
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 919
BglII AGATCT 1 cut(s) 436
Bme1390I CCNGG 3 cut(s) 24, 295, 396
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 1214
BmgT120I GGNCC 4 cut(s) 26, 391, 392, 698
BmiI GGNNCC 8 cut(s) 21, 64, 299, 392, 393, 552, 699, 874
BmrFI CCNGG 3 cut(s) 24, 295, 396
BmsI GCATC 8 cut(s) 192, 438, 488, 603, 819, 903, 947, 1033
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 2 cut(s) 786, 1082
Bpu10I CCTNAGC 1 cut(s) 607
BpuEI CTTGAG 1 cut(s) 523
BsaI GGTCTC 1 cut(s) 1226
BsaJI CCNNGG 6 cut(s) 15, 66, 72, 232, 394, 1215
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 4 cut(s) 560, 839, 1003, 1188
Bse1I ACTGG 1 cut(s) 1236
Bse21I CCTNAGG 1 cut(s) 1263
BseBI CCWGG 3 cut(s) 24, 295, 396
BseDI CCNNGG 6 cut(s) 15, 66, 72, 232, 394, 1215
BseGI GGATG 2 cut(s) 594, 1057
BseLI CCNNNNNNNGG 4 cut(s) 560, 839, 1003, 1188
BseMII CTCAG 4 cut(s) 621, 732, 1221, 1254
BseNI ACTGG 1 cut(s) 1236
BsePI GCGCGC 1 cut(s) 531
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 6 cut(s) 63, 92, 231, 337, 361, 365
BseYI CCCAGC 2 cut(s) 807, 1046
Bsh1236I CGCG 4 cut(s) 234, 332, 531, 533
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 5 cut(s) 71, 173, 393, 700, 1261
BshNI GGYRCC 1 cut(s) 872
BsiEI CGRYCG 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 1214
BsiSI CCGG 1 cut(s) 620
BslI CCNNNNNNNGG 4 cut(s) 560, 839, 1003, 1188
BsmAI GTCTC 4 cut(s) 886, 1051, 1098, 1226
BsmI GAATGC 4 cut(s) 964, 1069, 1109, 1111
BsnI GGCC 5 cut(s) 71, 173, 393, 700, 1261
Bso31I GGTCTC 1 cut(s) 1226
BsoBI CYCGRG 1 cut(s) 1214
Bsp120I GGGCCC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 8 cut(s) 19, 115, 367, 436, 575, 616, 993, 1165
BspANI GGCC 5 cut(s) 71, 173, 393, 700, 1261
BspCNI CTCAG 4 cut(s) 620, 733, 1222, 1255
BspFNI CGCG 4 cut(s) 234, 332, 531, 533
BspHI TCATGA 1 cut(s) 1039
BspLI GGNNCC 8 cut(s) 21, 64, 299, 392, 393, 552, 699, 874
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 6 cut(s) 14, 27, 583, 611, 988, 1160
BspQI GCTCTTC 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 872
BspTNI GGTCTC 1 cut(s) 1226
BsrI ACTGG 1 cut(s) 1236
BssECI CCNNGG 6 cut(s) 15, 66, 72, 232, 394, 1215
BssHII GCGCGC 1 cut(s) 531
BssMI GATC 8 cut(s) 19, 115, 367, 436, 575, 616, 993, 1165
Bst2UI CCWGG 3 cut(s) 24, 295, 396
Bst4CI ACNGT 1 cut(s) 906
Bst6I CTCTTC 4 cut(s) 339, 438, 488, 489
BstC8I GCNNGC 1 cut(s) 533
BstDEI CTNAG 4 cut(s) 607, 741, 1230, 1263
BstDSI CCRYGG 1 cut(s) 232
BstEII GGTNACC 1 cut(s) 906
BstF5I GGATG 2 cut(s) 594, 1057
BstFNI CGCG 4 cut(s) 234, 332, 531, 533
BstHHI GCGC 5 cut(s) 183, 332, 533, 535, 1138
BstKTI GATC 8 cut(s) 22, 118, 370, 439, 578, 619, 996, 1168
BstMAI GTCTC 4 cut(s) 886, 1051, 1098, 1226
BstMBI GATC 8 cut(s) 19, 115, 367, 436, 575, 616, 993, 1165
BstMCI CGRYCG 1 cut(s) 114
BstNI CCWGG 3 cut(s) 24, 295, 396
BstPI GGTNACC 1 cut(s) 906
BstSCI CCNGG 3 cut(s) 22, 293, 394
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 4 cut(s) 234, 332, 531, 533
BstV1I GCAGC 6 cut(s) 63, 92, 231, 337, 361, 365
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 3 cut(s) 19, 436, 575
BstYI RGATCY 3 cut(s) 19, 436, 575
Bsu36I CCTNAGG 1 cut(s) 1263
BsuRI GGCC 5 cut(s) 71, 173, 393, 700, 1261
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 2 cut(s) 594, 1057
BtsI GCAGTG 2 cut(s) 505, 1139
BtsIMutI CAGTG 3 cut(s) 505, 1139, 1243
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 1 cut(s) 533
CciI TCATGA 1 cut(s) 1039
CfoI GCGC 5 cut(s) 183, 332, 533, 535, 1138
Cfr13I GGNCC 4 cut(s) 26, 391, 392, 698
Cfr42I CCGCGG 1 cut(s) 235
Csp6I GTAC 1 cut(s) 291
CviAII CATG 4 cut(s) 755, 889, 1040, 1159
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 4 cut(s) 607, 741, 1230, 1263
DpnI GATC 8 cut(s) 21, 117, 369, 438, 577, 618, 995, 1167
DpnII GATC 8 cut(s) 19, 115, 367, 436, 575, 616, 993, 1165
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 4 cut(s) 339, 438, 488, 489
EarI CTCTTC 4 cut(s) 339, 438, 488, 489
EciI GGCGGA 1 cut(s) 75
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 395
Eco31I GGTCTC 1 cut(s) 1226
Eco47I GGWCC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 161
Eco81I CCTNAGG 1 cut(s) 1263
Eco88I CYCGRG 1 cut(s) 1214
Eco91I GGTNACC 1 cut(s) 906
EcoO109I RGGNCCY 3 cut(s) 391, 392, 698
EcoO65I GGTNACC 1 cut(s) 906
EcoRII CCWGG 3 cut(s) 22, 293, 394
EcoT22I ATGCAT 1 cut(s) 1111
EcoT38I GRGCYC 1 cut(s) 395
FaeI CATG 4 cut(s) 758, 892, 1043, 1162
FatI CATG 4 cut(s) 754, 888, 1039, 1158
FblI GTMKAC 1 cut(s) 1176
FokI GGATG 2 cut(s) 581, 1064
FriOI GRGCYC 1 cut(s) 395
FspBI CTAG 1 cut(s) 510
GlaI GCGC 5 cut(s) 182, 331, 532, 534, 1137
GsaI CCCAGC 2 cut(s) 811, 1050
GsuI CTGGAG 2 cut(s) 786, 1082
HaeIII GGCC 5 cut(s) 71, 173, 393, 700, 1261
HapII CCGG 1 cut(s) 620
HhaI GCGC 5 cut(s) 183, 332, 533, 535, 1138
Hin1II CATG 4 cut(s) 758, 892, 1043, 1162
Hin6I GCGC 5 cut(s) 181, 330, 531, 533, 1136
HinP1I GCGC 5 cut(s) 181, 330, 531, 533, 1136
HinfI GANTC 4 cut(s) 237, 803, 879, 1120
HpaII CCGG 1 cut(s) 620
HphI GGTGA 2 cut(s) 791, 900
Hpy166II GTNNAC 3 cut(s) 255, 782, 1177
Hpy188I TCNGA 7 cut(s) 19, 30, 76, 405, 580, 742, 817
Hpy188III TCNNGA 6 cut(s) 440, 510, 609, 1040, 1099, 1214
Hpy8I GTNNAC 3 cut(s) 255, 782, 1177
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 310, 822, 848
HpyCH4III ACNGT 1 cut(s) 906
HpyCH4IV ACGT 2 cut(s) 647, 835
HpyCH4V TGCA 8 cut(s) 168, 353, 451, 479, 654, 962, 1109, 1241
HpyF3I CTNAG 4 cut(s) 607, 741, 1230, 1263
HpySE526I ACGT 2 cut(s) 647, 835
Hsp92II CATG 4 cut(s) 758, 892, 1043, 1162
HspAI GCGC 5 cut(s) 181, 330, 531, 533, 1136
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 8 cut(s) 19, 115, 367, 436, 575, 616, 993, 1165
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 4 cut(s) 62, 227, 550, 1207
Lsp1109I GCAGC 6 cut(s) 63, 92, 231, 337, 361, 365
LweI GCATC 8 cut(s) 192, 438, 488, 603, 819, 903, 947, 1033
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 647, 835
MaeIII GTNAC 7 cut(s) 238, 263, 418, 680, 900, 906, 1219
MalI GATC 8 cut(s) 21, 117, 369, 438, 577, 618, 995, 1167
MboI GATC 8 cut(s) 19, 115, 367, 436, 575, 616, 993, 1165
MboII GAAGA 7 cut(s) 43, 154, 356, 455, 476, 505, 1202
MfeI CAATTG 2 cut(s) 122, 1020
MflI RGATCY 3 cut(s) 19, 436, 575
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 5 cut(s) 122, 409, 635, 942, 1020
MlyI GAGTC 3 cut(s) 246, 888, 1114
Mph1103I ATGCAT 1 cut(s) 1111
MroXI GAANNNNTTC 1 cut(s) 1109
MseI TTAA 4 cut(s) 408, 674, 786, 945
MslI CAYNNNNRTG 2 cut(s) 796, 870
MspA1I CMGCKG 4 cut(s) 54, 83, 209, 234
MspI CCGG 1 cut(s) 620
MspR9I CCNGG 3 cut(s) 24, 295, 396
MunI CAATTG 2 cut(s) 122, 1020
Mva1269I GAATGC 4 cut(s) 964, 1069, 1109, 1111
MvaI CCWGG 3 cut(s) 24, 295, 396
MvnI CGCG 4 cut(s) 234, 332, 531, 533
NdeII GATC 8 cut(s) 19, 115, 367, 436, 575, 616, 993, 1165
NlaIII CATG 4 cut(s) 758, 892, 1043, 1162
NlaIV GGNNCC 8 cut(s) 21, 64, 299, 392, 393, 552, 699, 874
NmeAIII GCCGAG 2 cut(s) 91, 223
NmuCI GTSAC 3 cut(s) 238, 900, 906
NsiI ATGCAT 1 cut(s) 1111
PagI TCATGA 1 cut(s) 1039
PaqCI CACCTGC 1 cut(s) 919
PauI GCGCGC 1 cut(s) 531
PceI AGGCCT 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 488
PctI GAATGC 4 cut(s) 964, 1069, 1109, 1111
PdmI GAANNNNTTC 1 cut(s) 1109
PfeI GAWTC 1 cut(s) 803
PflMI CCANNNNNTGG 2 cut(s) 560, 1188
PleI GAGTC 3 cut(s) 245, 887, 1114
PpsI GAGTC 3 cut(s) 245, 887, 1114
PshBI ATTAAT 1 cut(s) 408
PsiI TTATAA 1 cut(s) 918
Psp1406I AACGTT 1 cut(s) 647
Psp6I CCWGG 3 cut(s) 22, 293, 394
PspEI GGTNACC 1 cut(s) 906
PspFI CCCAGC 2 cut(s) 807, 1046
PspGI CCWGG 3 cut(s) 22, 293, 394
PspN4I GGNNCC 8 cut(s) 21, 64, 299, 392, 393, 552, 699, 874
PspOMI GGGCCC 1 cut(s) 391
PspPI GGNCC 4 cut(s) 26, 391, 392, 698
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 3 cut(s) 19, 436, 575
PteI GCGCGC 1 cut(s) 531
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
RseI CAYNNNNRTG 2 cut(s) 796, 870
SacII CCGCGG 1 cut(s) 235
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 4 cut(s) 408, 674, 786, 945
Sau3AI GATC 8 cut(s) 19, 115, 367, 436, 575, 616, 993, 1165
Sau96I GGNCC 4 cut(s) 26, 391, 392, 698
SchI GAGTC 3 cut(s) 246, 888, 1114
ScrFI CCNGG 3 cut(s) 24, 295, 396
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 8 cut(s) 192, 438, 488, 603, 819, 903, 947, 1033
SfcI CTRYAG 1 cut(s) 351
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 2 cut(s) 796, 870
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
Sse9I AATT 5 cut(s) 122, 409, 635, 942, 1020
SseBI AGGCCT 1 cut(s) 71
SspI AATATT 1 cut(s) 386
SspMI CTAG 1 cut(s) 510
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 22, 293, 394
TaaI ACNGT 1 cut(s) 906
TaiI ACGT 2 cut(s) 650, 838
TaqI TCGA 1 cut(s) 441
TaqII GACCGA 2 cut(s) 50, 128
TasI AATT 5 cut(s) 122, 409, 635, 942, 1020
TfiI GAWTC 1 cut(s) 803
Tru1I TTAA 4 cut(s) 408, 674, 786, 945
Tru9I TTAA 4 cut(s) 408, 674, 786, 945
TscAI CASTG 3 cut(s) 505, 1146, 1243
TseFI GTSAC 3 cut(s) 238, 900, 906
TseI GCWGC 6 cut(s) 51, 80, 219, 350, 353, 374
Tsp45I GTSAC 3 cut(s) 238, 900, 906
TspDTI ATGAA 4 cut(s) 357, 648, 993, 1059
TspGWI ACGGA 1 cut(s) 1113
TspRI CASTG 3 cut(s) 505, 1146, 1243
Van91I CCANNNNNTGG 2 cut(s) 560, 1188
VpaK11BI GGWCC 1 cut(s) 26
VspI ATTAAT 1 cut(s) 408
XapI RAATTY 1 cut(s) 635
XbaI TCTAGA 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 1176
XmnI GAANNNNTTC 1 cut(s) 1109
XspI CTAG 1 cut(s) 510
Zsp2I ATGCAT 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.