MD12G1168800.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002511
Physical Location & Seq
Reverse (-)
24907241 .. 24916003
8763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1168800.v1.1.491

Sequence Viewer

Length: 3378 bp
ATGTTCGCCAAGCGGCTTTTGCAGAAAGCCATAAACAACTCTCAGCATAATTTGCCACACGGGAGTTTGACGGCGGAAGACTTGGACCTACGAGTTGCAGTTCACTATGGCATCCCATCCACGGCGTCCATTCTTGCTTTCGACCCGATTCAGCGCCTTTTGGCCATTGGAACATTGGATGGGAGGATAAAAGTAATCGGTGGTGATGGTATCGAAGGACTCTTTATATCCCGAAGCAACTGCCTTACAAATACATTGAGGTATCTGTGTGCACCATTCATTCCCCTTTTTCTACAAAACCAGGGTTATTTGGTGAGCATCTTGAATGATAATGATATTCAGGTTTGGAATCTTGAGAGCAGGTCCCTAGTTTATTGTCTAGAATGGGAAACAAATATAACCGCTTTTTCTGTAATCCATGGCTCCAGTTTAATGTATGTTGGCGATGAGTATGCTCTAGTGGCTGTGATGAAGTATGATGCAGAGGAGGGAAAATTATTGCAATTGCCATATCATATTTCTGCAAATTCTTTAAGTGAAGCTGCTGAGTTTCCATTTCCAACTGATCAACCTATAGTTGGAATTCTTCCCCAACCCTGTTCGTCTGGAAATAGAGTATTGATCGCATATCAAAACGGGGTGGTTGTTCTTTGGGATGTATCTGAAGCTAAAATAGTTTATATTGGAGGTGGTAAGGATCTCCAACTAAAAGATGGAATTGTTAAATCTACAAATGAAATCGATGTAGATTGTCCAGAAGATACATTGGAACATCAACTGGGAGACAAAGAGATAAGTGCTCTTTGTTGGGCATCTTTGAATGGGTCCATTCTGGCTGTGGGATACATAGATGGAGACATTTTATTCTGGAACACGTCATCTCCTCCTTCCGTCAAGGGTCAAAAAGCATTATCGCCATCTAATAATGTTGTGAAGTTACGATTATCATCAGCTGAAAGGAGACTTCCTGTCATAGTTTTACAGTGGTCCACAGATTATAAATCCCATAATGGTTGTGATGGCCAACTATTTATCTATGGCGGCGACGAAATCGGATCAGAAGAAGTTTTGACGGTTTTAACTCTTGAATGGTCACCTGGAATGGGGAATTTAAGATGTGTTGGTCGTACAGACCTTACCCTTACTGGCTCTTTTGCAGACATGATTTTGTTACAAAGTTCTGGGACAACTGGGGGTAATCACAAAGCTGATGTTTTTGTATTAACCAACCCTGGGCAACTGCATCTTTATGATTATGCTACCCTCTCTGCCTTAATGTCCCAGAAAGAAAGGAATCCATCTGTATCTGCTTTGGACTTTCCGGTGGTGATACCTACAACTAATCCAACAATGACCGATGCCAAACTTATTAGGGTACCCACTGGGGAGAACTTATTGAAAGCTATGTCAGAGATGTCCTCAGTTGGGAACCTTGGCTCAGCACAAACCCAATCAGCTGGTACAAAATGGCCCTTGACTGGGGGTGTTCCCAGTCAACTGTCTATTTCTGAGAATAATGGTATTGAGAGACTGTACCTAGCAGGATATTCAGATGGATCTGTTCGGATATGGAATGCCACATATCCACTCTTATCTTGTATCTGCCTTGTAGAAAGTGAGGTGCAAGGTATAGAAGTTGCTGGTTCAAGTGCTCCCGTAACACGACTGGATTTCTGCGCCTTCACTTTAAATCTGGCTGTTGGCAATGAATGTGGTCTGGTTCGAATTTATAACGTGAAAGGCTGTTCAGATGGGGTAAAGTTCATCTTTGTCACAGAAACCAAATATGAAGTTCACGAATCGCCTCAAGCAAAAGGACCGCGATGCAGAGCTGTATTTAGTCTTACAAATTCCCCAGTTCAAGCGCTTCAGTTTGTAAAGCATGGAGGTAAACTTGCTGTGGGATTTGAGTGTGGTCATGTCGCAGTGCTGGACGCAAGTTCCTTTTCTGTATTGTTCTATATCAAAGATGCATCTTTCTCTAGCTCCCCAGTCATTTCAATGATTTGGAAAGAACTTACAAATTCTCAAGGCCTCCTGAAGAGCCCAAAGGTCTCTGGAACAAAGTCGACAGTTAGCCCTGTAGAAGAAGTAATATTCATCTTAACCAAGGATGCAAATATACATGTAATTGATGGAAACACCGGTAACCTGATCATCCCTCAGCCATGGCACTTGAAAAAGGAGTCAATTGCAATTTCAATGTATGTTATAGATGGCAGATTATCTGCCTCTAAAGTATCCAATGATAACCCGCCTACAGAGGCCGCCAAGGATTCAACCAAAAATGACCCTGTGCCAGGAAGTCTTTCAGTTGTTATAAATTCACCTGACACTGACGATGACAACTATTCAGAAAATACATACTCTGAAGAGAGATTGTTGAATTCATTCATTTTGCTTTGTTGTGTGGATTCACTGCACTTGTACTCGACAAAATCTGTGATTCAGGGAAACAATAAACCCATCCGAAAAAAGAAACATACAAGACCTTGCATTTGGACTGCAACTTTTAAGAAAGTTGAAAAGGTTTCAGGATTGGCTTTGCTGTTTCAGACTGGAGAAATTGAGATCAGATCTATTCCTGATTTAGAATTGGTGAAAGAAAGCTCCTTGCTGTCAATTCTAAGGTGGAATTGCAAGGCAAACATGGATAAAACAATGAGTTCCGATGATGCACATATTACGCTGGCAAATGGGTATGAATCGGCATTCATCTCTATGTTAGCTATTGAGAATGATTTCAGGATTCCAGAGTCTTTGCCCTGTCTCCATGATACAGTTGTTGCAGCTGCTGCAGACGCTGCACTCAGTGTCTCTGTGAATCAGAAGAAAAAACAGGGTACTGCATCTGGGATTTTAGGTGGTATTGTCAAGGGCTTTAAGGGAGGAAAAATGGTCCAGAGTGGAGATCCTGCAGCAGTTCTTAAATCAACTTTCGATCGTTTAGAGGGCACATTTTCAAAGTCCCAACTGTCAGAACCATCCCCGCCTGTAGACCATCAAGAAGTTGTGGAGCTTAATATAGATGACATTGAAATTGATGAACCTCTATATGTCGCATCTACTTCGTCACCACATGATGTCAAAGATAAAAAGAGAGAAGGGGAATCAGAAAGGGAAAAGTTATTTGAAGGTGGTACTGGCGATACAAAGCCCAGAGTTAGAACGGCCGAAGAAATTAGAGCTAAATATAGAAAGGCTGAGGGTGCTTCATCTGCGGCTTCTCAAGCGCGAAATAAGCTCATGGAAAGGCAAGAAAAACTTGAGAAAATCAGCCGGCGTACTGAAGATCTGCAGAATGGGGCTGAAGACTTCGCATCACTGGCAAACGAGCTTGTGAAGACATTGGAAGCTCGAAAATGGTGGCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000137 GO:0000139 GO:0000902 GO:0000904 GO:0002791 GO:0003674 GO:0005096 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005795 GO:0005802 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006892 GO:0006893 GO:0006996 GO:0007010 GO:0007275 GO:0007399 GO:0007409 GO:0008047 GO:0008150 GO:0009653 GO:0009987 GO:0010008 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0017016 GO:0017137 GO:0017157 GO:0019899 GO:0019900 GO:0019901 GO:0022008 GO:0022607 GO:0030029 GO:0030030 GO:0030036 GO:0030154 GO:0030182 GO:0030234 GO:0030695 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0031090 GO:0031175 GO:0031267 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0031985 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048193 GO:0048468 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050708 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051046 GO:0051049 GO:0051179 GO:0051223 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0060627 GO:0061564 GO:0065003 GO:0065007 GO:0065009 GO:0070201 GO:0071840 GO:0071944 GO:0090087 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0098876 GO:0099568 GO:0120036 GO:0120039 GO:1903530
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 Synaptobrevin

Protein Analysis

1126

Amino Acids

122.56

Weight (kDa)

5.42

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 999, 1731, 2321
Acc36I ACCTGC 1 cut(s) 351
Acc65I GGTACC 1 cut(s) 1375
AccB1I GGYRCC 1 cut(s) 1375
AccI GTMKAC 2 cut(s) 2069, 2997
AccII CGCG 2 cut(s) 1822, 3235
AciI CCGC 9 cut(s) 13, 74, 402, 1041, 1820, 2255, 2268, 2990, 3221
AclWI GGATC 4 cut(s) 705, 1063, 1564, 2906
AcoI YGGCCR 3 cut(s) 162, 1021, 3171
AcsI RAATTY 9 cut(s) 526, 582, 1108, 1725, 1849, 2023, 2323, 2386, 3372
AcuI CTGAAG 6 cut(s) 684, 1853, 2060, 2391, 3309, 3330
AcyI GRCGYC 1 cut(s) 125
AdeI CACNNNGTG 1 cut(s) 2813
AfaI GTAC 8 cut(s) 1129, 1377, 1462, 1535, 2429, 2845, 3142, 3286
AfeI AGCGCT 1 cut(s) 1866
AfiI CCNNNNNNNGG 8 cut(s) 121, 578, 1103, 1233, 1425, 1478, 1479, 2300
AflIII ACRYGT 2 cut(s) 873, 2125
AgeI ACCGGT 1 cut(s) 2144
AhdI GACNNNNNGTC 1 cut(s) 968
AjiI CACGTC 1 cut(s) 876
AjnI CCWGG 4 cut(s) 300, 1096, 1231, 2299
Alw21I GWGCWC 3 cut(s) 274, 802, 1654
Alw26I GTCTC 7 cut(s) 777, 849, 955, 1522, 2059, 2774, 2821
Alw44I GTGCAC 1 cut(s) 270
AlwI GGATC 4 cut(s) 705, 1063, 1564, 2906
AlwNI CAGNNNCTG 2 cut(s) 2795, 2804
Aor51HI AGCGCT 1 cut(s) 1866
AoxI GGCC 6 cut(s) 162, 1021, 1469, 2032, 2265, 3171
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 6 cut(s) 542, 2789, 2792, 2795, 2804, 2918
ApoI RAATTY 9 cut(s) 526, 582, 1108, 1725, 1849, 2023, 2323, 2386, 3372
ArsI GACNNNNNNTTYG 2 cut(s) 2481, 2513
AsiGI ACCGGT 1 cut(s) 2144
Asp700I GAANNNNTTC 3 cut(s) 2308, 2390, 2741
Asp718I GGTACC 1 cut(s) 1375
AspLEI GCGC 4 cut(s) 156, 1679, 1867, 3235
AspS9I GGNCC 7 cut(s) 85, 363, 825, 987, 1470, 1817, 2899
AsuHPI GGTGA 7 cut(s) 215, 325, 1086, 1339, 2319, 2611, 3066
AsuII TTCGAA 1 cut(s) 1723
AvaII GGWCC 6 cut(s) 85, 363, 825, 987, 1817, 2899
BaeGI GKGCMC 2 cut(s) 274, 2957
BaeI ACNNNNGTAYC 2 cut(s) 2769, 2802
BalI TGGCCA 2 cut(s) 164, 1023
BanI GGYRCC 1 cut(s) 1375
BanII GRGCYC 1 cut(s) 2048
BbsI GAAGAC 3 cut(s) 84, 3318, 3350
Bbv12I GWGCWC 3 cut(s) 274, 802, 1654
BbvCI CCTCAGC 2 cut(s) 2163, 3204
BbvI GCAGC 6 cut(s) 529, 2779, 2782, 2791, 2801, 2930
BceAI ACGGC 3 cut(s) 87, 138, 3186
BcgI CGANNNNNNTGC 6 cut(s) 222, 256, 434, 468, 3051, 3085
BciT130I CCWGG 4 cut(s) 302, 1098, 1233, 2301
BciVI GTATCC 2 cut(s) 836, 2251
BclI TGATCA 2 cut(s) 565, 2154
BcoDI GTCTC 7 cut(s) 777, 849, 955, 1522, 2059, 2774, 2821
BfaI CTAG 5 cut(s) 368, 380, 458, 1538, 1983
BfmI CTRYAG 7 cut(s) 573, 2082, 2259, 2796, 2916, 2994, 3296
BfoI RGCGCY 2 cut(s) 157, 1868
BfuAI ACCTGC 1 cut(s) 351
BfuI GTATCC 2 cut(s) 836, 2251
BglII AGATCT 2 cut(s) 2576, 3292
BlpI GCTNAGC 1 cut(s) 1438
Bme1390I CCNGG 4 cut(s) 302, 1098, 1233, 2301
Bme18I GGWCC 6 cut(s) 85, 363, 825, 987, 1817, 2899
BmeRI GACNNNNNGTC 1 cut(s) 968
BmgBI CACGTC 1 cut(s) 876
BmgT120I GGNCC 7 cut(s) 85, 363, 825, 987, 1470, 1817, 2899
BmiI GGNNCC 5 cut(s) 365, 424, 826, 1377, 1430
BmrFI CCNGG 4 cut(s) 302, 1098, 1233, 2301
BmrI ACTGGG 7 cut(s) 788, 1200, 1392, 1485, 1488, 1850, 1985
BmuI ACTGGG 7 cut(s) 788, 1200, 1392, 1485, 1488, 1850, 1985
BpiI GAAGAC 3 cut(s) 84, 3318, 3350
BplI GAGNNNNNCTC 2 cut(s) 1403, 1435
BpmI CTGGAG 2 cut(s) 409, 2580
Bpu10I CCTNAGC 2 cut(s) 2163, 3204
Bpu1102I GCTNAGC 1 cut(s) 1438
Bpu14I TTCGAA 1 cut(s) 1723
BpuEI CTTGAG 5 cut(s) 374, 1791, 2013, 3213, 3287
Bsa29I ATCGAT 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 946
BsaHI GRCGYC 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 2059
BsaJI CCNNGG 9 cut(s) 120, 301, 418, 1231, 1232, 1432, 2109, 2168, 2271
BsaWI WCCGGW 2 cut(s) 1321, 2144
BsaXI ACNNNNNCTCC 2 cut(s) 865, 895
Bsc4I CCNNNNNNNGG 8 cut(s) 121, 578, 1103, 1233, 1425, 1478, 1479, 2300
Bse118I RCCGGY 2 cut(s) 2144, 3279
Bse3DI GCAATG 1 cut(s) 1711
Bse8I GATNNNNATC 1 cut(s) 946
BseBI CCWGG 4 cut(s) 302, 1098, 1233, 2301
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 9 cut(s) 120, 301, 418, 1231, 1232, 1432, 2109, 2168, 2271
BseGI GGATG 8 cut(s) 111, 116, 184, 661, 2119, 2157, 2466, 2984
BseJI GATNNNNATC 1 cut(s) 946
BseLI CCNNNNNNNGG 8 cut(s) 121, 578, 1103, 1233, 1425, 1478, 1479, 2300
BseMI GCAATG 1 cut(s) 1711
BseMII CTCAG 8 cut(s) 56, 537, 1434, 1452, 1500, 2177, 2824, 3195
BseRI GAGGAG 2 cut(s) 500, 873
BseSI GKGCMC 2 cut(s) 274, 2957
BseX3I CGGCCG 1 cut(s) 3171
BseXI GCAGC 6 cut(s) 529, 2779, 2782, 2791, 2801, 2930
BsgI GTGCAG 2 cut(s) 2405, 2790
Bsh1236I CGCG 2 cut(s) 1822, 3235
Bsh1285I CGRYCG 2 cut(s) 2944, 3174
BshFI GGCC 6 cut(s) 164, 1023, 1471, 2034, 2267, 3173
BshNI GGYRCC 1 cut(s) 1375
BshTI ACCGGT 1 cut(s) 2144
BshVI ATCGAT 1 cut(s) 741
BsiEI CGRYCG 2 cut(s) 2944, 3174
BsiHKAI GWGCWC 3 cut(s) 274, 802, 1654
BsiSI CCGG 3 cut(s) 1322, 2145, 3280
BslFI GGGAC 4 cut(s) 349, 1198, 1264, 2953
BslI CCNNNNNNNGG 8 cut(s) 121, 578, 1103, 1233, 1425, 1478, 1479, 2300
BsmAI GTCTC 7 cut(s) 777, 849, 955, 1522, 2059, 2774, 2821
BsmFI GGGAC 4 cut(s) 349, 1198, 1264, 2953
BsmI GAATGC 2 cut(s) 1579, 2711
BsnI GGCC 6 cut(s) 164, 1023, 1471, 2034, 2267, 3173
Bso31I GGTCTC 1 cut(s) 2059
Bsp119I TTCGAA 1 cut(s) 1723
Bsp1286I GDGCHC 5 cut(s) 274, 802, 1654, 2048, 2957
Bsp1720I GCTNAGC 1 cut(s) 1438
Bsp19I CCATGG 2 cut(s) 418, 2168
BspACI CCGC 9 cut(s) 13, 74, 402, 1041, 1820, 2255, 2268, 2990, 3221
BspANI GGCC 6 cut(s) 164, 1023, 1471, 2034, 2267, 3173
BspCNI CTCAG 8 cut(s) 55, 538, 1433, 1451, 1501, 2176, 2823, 3196
BspDI ATCGAT 1 cut(s) 741
BspFNI CGCG 2 cut(s) 1822, 3235
BspLI GGNNCC 5 cut(s) 365, 424, 826, 1377, 1430
BspMAI CTGCAG 3 cut(s) 2800, 2920, 3300
BspMI ACCTGC 1 cut(s) 351
BspPI GGATC 4 cut(s) 705, 1063, 1564, 2906
BspQI GCTCTTC 1 cut(s) 2036
BspT104I TTCGAA 1 cut(s) 1723
BspT107I GGYRCC 1 cut(s) 1375
BspTNI GGTCTC 1 cut(s) 2059
BsrDI GCAATG 1 cut(s) 1711
BsrFI RCCGGY 2 cut(s) 2144, 3279
BssAI RCCGGY 2 cut(s) 2144, 3279
BssECI CCNNGG 9 cut(s) 120, 301, 418, 1231, 1232, 1432, 2109, 2168, 2271
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 5 cut(s) 418, 1432, 2109, 2168, 2271
Bst2UI CCWGG 4 cut(s) 302, 1098, 1233, 2301
Bst4CI ACNGT 7 cut(s) 984, 1075, 1500, 1533, 2074, 2782, 2976
Bst6I CTCTTC 2 cut(s) 2036, 2367
BstACI GRCGYC 1 cut(s) 125
BstAPI GCANNNNNTGC 3 cut(s) 52, 2795, 2804
BstBI TTCGAA 1 cut(s) 1723
BstC8I GCNNGC 2 cut(s) 2691, 3281
BstDEI CTNAG 9 cut(s) 42, 546, 1420, 1438, 1509, 2163, 2627, 2810, 3204
BstDSI CCRYGG 3 cut(s) 120, 418, 2168
BstEII GGTNACC 2 cut(s) 1092, 2147
BstENI CCTNNNNNAGG 1 cut(s) 2298
BstF5I GGATG 8 cut(s) 111, 116, 184, 661, 2119, 2157, 2466, 2984
BstFNI CGCG 2 cut(s) 1822, 3235
BstH2I RGCGCY 2 cut(s) 157, 1868
BstHHI GCGC 4 cut(s) 156, 1679, 1867, 3235
BstMAI GTCTC 7 cut(s) 777, 849, 955, 1522, 2059, 2774, 2821
BstMCI CGRYCG 2 cut(s) 2944, 3174
BstNI CCWGG 4 cut(s) 302, 1098, 1233, 2301
BstNSI RCATGY 1 cut(s) 2129
BstPI GGTNACC 2 cut(s) 1092, 2147
BstSCI CCNGG 4 cut(s) 300, 1096, 1231, 2299
BstSFI CTRYAG 7 cut(s) 573, 2082, 2259, 2796, 2916, 2994, 3296
BstSLI GKGCMC 2 cut(s) 274, 2957
BstUI CGCG 2 cut(s) 1822, 3235
BstV1I GCAGC 6 cut(s) 529, 2779, 2782, 2791, 2801, 2930
BstV2I GAAGAC 3 cut(s) 84, 3318, 3350
BstX2I RGATCY 5 cut(s) 697, 1556, 2576, 2911, 3292
BstXI CCANNNNNNTGG 1 cut(s) 1457
BstYI RGATCY 5 cut(s) 697, 1556, 2576, 2911, 3292
BstZI CGGCCG 1 cut(s) 3171
Bsu15I ATCGAT 1 cut(s) 741
BsuI GTATCC 2 cut(s) 836, 2251
BsuRI GGCC 6 cut(s) 164, 1023, 1471, 2034, 2267, 3173
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 3 cut(s) 120, 418, 2168
BtgZI GCGATG 2 cut(s) 459, 1837
BtrI CACGTC 1 cut(s) 876
BtsCI GGATG 8 cut(s) 111, 116, 184, 661, 2119, 2157, 2466, 2984
BtsI GCAGTG 2 cut(s) 1932, 2417
BtsIMutI CAGTG 7 cut(s) 989, 1380, 1932, 2334, 2417, 2818, 3323
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 2691, 3281
CaiI CAGNNNCTG 2 cut(s) 2795, 2804
CfoI GCGC 4 cut(s) 156, 1679, 1867, 3235
Cfr10I RCCGGY 2 cut(s) 2144, 3279
Cfr13I GGNCC 7 cut(s) 85, 363, 825, 987, 1470, 1817, 2899
ClaI ATCGAT 1 cut(s) 741
CseI GACGC 3 cut(s) 114, 1943, 2810
Csp6I GTAC 8 cut(s) 1128, 1376, 1461, 1534, 2428, 2844, 3141, 3285
CspAI ACCGGT 1 cut(s) 2144
CspCI CAANNNNNGTGG 2 cut(s) 1693, 1728
CviQI GTAC 8 cut(s) 1128, 1376, 1461, 1534, 2428, 2844, 3141, 3285
DdeI CTNAG 9 cut(s) 42, 546, 1420, 1438, 1509, 2163, 2627, 2810, 3204
DraI TTTAAA 1 cut(s) 1689
DraIII CACNNNGTG 1 cut(s) 2813
DriI GACNNNNNGTC 1 cut(s) 968
EaeI YGGCCR 3 cut(s) 162, 1021, 3171
EagI CGGCCG 1 cut(s) 3171
Eam1104I CTCTTC 2 cut(s) 2036, 2367
Eam1105I GACNNNNNGTC 1 cut(s) 968
EarI CTCTTC 2 cut(s) 2036, 2367
EciI GGCGGA 1 cut(s) 89
EclXI CGGCCG 1 cut(s) 3171
Eco130I CCWWGG 5 cut(s) 418, 1432, 2109, 2168, 2271
Eco147I AGGCCT 1 cut(s) 2034
Eco24I GRGCYC 1 cut(s) 2048
Eco31I GGTCTC 1 cut(s) 2059
Eco47I GGWCC 6 cut(s) 85, 363, 825, 987, 1817, 2899
Eco47III AGCGCT 1 cut(s) 1866
Eco52I CGGCCG 1 cut(s) 3171
Eco57I CTGAAG 6 cut(s) 684, 1853, 2060, 2391, 3309, 3330
Eco91I GGTNACC 2 cut(s) 1092, 2147
EcoNI CCTNNNNNAGG 1 cut(s) 2298
EcoO109I RGGNCCY 1 cut(s) 363
EcoO65I GGTNACC 2 cut(s) 1092, 2147
EcoRI GAATTC 2 cut(s) 582, 2386
EcoRII CCWGG 4 cut(s) 300, 1096, 1231, 2299
EcoT14I CCWWGG 5 cut(s) 418, 1432, 2109, 2168, 2271
EcoT22I ATGCAT 1 cut(s) 1975
EcoT38I GRGCYC 1 cut(s) 2048
ErhI CCWWGG 5 cut(s) 418, 1432, 2109, 2168, 2271
FalI AAGNNNNNCTT 4 cut(s) 1751, 1783, 3249, 3281
FaqI GGGAC 4 cut(s) 349, 1198, 1264, 2953
FauI CCCGC 2 cut(s) 2262, 2997
FbaI TGATCA 2 cut(s) 565, 2154
FblI GTMKAC 2 cut(s) 2069, 2997
FokI GGATG 8 cut(s) 98, 103, 191, 668, 2126, 2144, 2453, 2971
FriOI GRGCYC 1 cut(s) 2048
FspBI CTAG 5 cut(s) 368, 380, 458, 1538, 1983
GlaI GCGC 4 cut(s) 155, 1678, 1866, 3234
GsuI CTGGAG 2 cut(s) 409, 2580
HaeII RGCGCY 2 cut(s) 157, 1868
HaeIII GGCC 6 cut(s) 164, 1023, 1471, 2034, 2267, 3173
HapII CCGG 3 cut(s) 1322, 2145, 3280
HgaI GACGC 3 cut(s) 114, 1943, 2810
HhaI GCGC 4 cut(s) 156, 1679, 1867, 3235
Hin1I GRCGYC 1 cut(s) 125
Hin6I GCGC 4 cut(s) 154, 1677, 1865, 3233
HinP1I GCGC 4 cut(s) 154, 1677, 1865, 3233
HincII GTYRAC 2 cut(s) 1496, 2070
HindII GTYRAC 2 cut(s) 1496, 2070
HpaII CCGG 3 cut(s) 1322, 2145, 3280
HphI GGTGA 7 cut(s) 215, 325, 1086, 1339, 2319, 2611, 3066
Hpy166II GTNNAC 8 cut(s) 103, 272, 990, 1496, 1795, 1892, 2070, 2998
Hpy8I GTNNAC 8 cut(s) 103, 272, 990, 1496, 1795, 1892, 2070, 2998
Hpy99I CGWCG 1 cut(s) 1049
HpyAV CCTTC 5 cut(s) 209, 897, 1690, 3098, 3128
HpyCH4III ACNGT 7 cut(s) 984, 1075, 1500, 1533, 2074, 2782, 2976
HpyCH4IV ACGT 2 cut(s) 875, 1734
HpyF3I CTNAG 9 cut(s) 42, 546, 1420, 1438, 1509, 2163, 2627, 2810, 3204
HpySE526I ACGT 2 cut(s) 875, 1734
Hsp92I GRCGYC 1 cut(s) 125
HspAI GCGC 4 cut(s) 154, 1677, 1865, 3233
KpnI GGTACC 1 cut(s) 1379
KroI GCCGGC 1 cut(s) 3279
KroNI GCCGGC 1 cut(s) 3281
Ksp22I TGATCA 2 cut(s) 565, 2154
LguI GCTCTTC 1 cut(s) 2036
LmnI GCTCC 5 cut(s) 428, 1657, 1991, 2615, 3016
Lsp1109I GCAGC 6 cut(s) 529, 2779, 2782, 2791, 2801, 2930
MaeI CTAG 5 cut(s) 368, 380, 458, 1538, 1983
MaeII ACGT 2 cut(s) 875, 1734
MaeIII GTNAC 7 cut(s) 936, 1092, 1170, 1657, 1771, 2147, 3072
MfeI CAATTG 2 cut(s) 503, 2190
MflI RGATCY 5 cut(s) 697, 1556, 2576, 2911, 3292
MhlI GDGCHC 5 cut(s) 274, 802, 1654, 2048, 2957
MlsI TGGCCA 2 cut(s) 164, 1023
MluNI TGGCCA 2 cut(s) 164, 1023
MlyI GAGTC 3 cut(s) 213, 2195, 2765
MmeI TCCRAC 4 cut(s) 559, 584, 727, 1370
Mox20I TGGCCA 2 cut(s) 164, 1023
Mph1103I ATGCAT 1 cut(s) 1975
MroNI GCCGGC 1 cut(s) 3279
MroXI GAANNNNTTC 3 cut(s) 2308, 2390, 2741
MscI TGGCCA 2 cut(s) 164, 1023
MslI CAYNNNNRTG 3 cut(s) 1248, 2000, 2720
Msp20I TGGCCA 2 cut(s) 164, 1023
MspA1I CMGCKG 3 cut(s) 953, 1457, 2792
MspI CCGG 3 cut(s) 1322, 2145, 3280
MspR9I CCNGG 4 cut(s) 302, 1098, 1233, 2301
MunI CAATTG 2 cut(s) 503, 2190
Mva1269I GAATGC 2 cut(s) 1579, 2711
MvaI CCWGG 4 cut(s) 302, 1098, 1233, 2301
MvnI CGCG 2 cut(s) 1822, 3235
NaeI GCCGGC 1 cut(s) 3281
NcoI CCATGG 2 cut(s) 418, 2168
NgoMIV GCCGGC 1 cut(s) 3279
NlaIV GGNNCC 5 cut(s) 365, 424, 826, 1377, 1430
NmuCI GTSAC 3 cut(s) 1092, 1771, 3072
NsiI ATGCAT 1 cut(s) 1975
NspI RCATGY 1 cut(s) 2129
NspV TTCGAA 1 cut(s) 1723
PasI CCCWGGG 1 cut(s) 1232
PceI AGGCCT 1 cut(s) 2034
PciI ACATGT 1 cut(s) 2125
PciSI GCTCTTC 1 cut(s) 2036
PctI GAATGC 2 cut(s) 1579, 2711
PdiI GCCGGC 1 cut(s) 3281
PdmI GAANNNNTTC 3 cut(s) 2308, 2390, 2741
PinAI ACCGGT 1 cut(s) 2144
Ple19I CGATCG 1 cut(s) 2944
PleI GAGTC 3 cut(s) 213, 2194, 2764
PpsI GAGTC 3 cut(s) 213, 2194, 2764
PpuMI RGGWCCY 1 cut(s) 363
PscI ACATGT 1 cut(s) 2125
PsiI TTATAA 3 cut(s) 999, 1731, 2321
Psp5II RGGWCCY 1 cut(s) 363
Psp6I CCWGG 4 cut(s) 300, 1096, 1231, 2299
PspEI GGTNACC 2 cut(s) 1092, 2147
PspGI CCWGG 4 cut(s) 300, 1096, 1231, 2299
PspN4I GGNNCC 5 cut(s) 365, 424, 826, 1377, 1430
PspPI GGNCC 7 cut(s) 85, 363, 825, 987, 1470, 1817, 2899
PspPPI RGGWCCY 1 cut(s) 363
PstI CTGCAG 3 cut(s) 2800, 2920, 3300
PstNI CAGNNNCTG 2 cut(s) 2795, 2804
PsuI RGATCY 5 cut(s) 697, 1556, 2576, 2911, 3292
PvuI CGATCG 1 cut(s) 2944
PvuII CAGCTG 3 cut(s) 953, 1457, 2792
RsaI GTAC 8 cut(s) 1129, 1377, 1462, 1535, 2429, 2845, 3142, 3286
RsaNI GTAC 8 cut(s) 1128, 1376, 1461, 1534, 2428, 2844, 3141, 3285
RseI CAYNNNNRTG 3 cut(s) 1248, 2000, 2720
SalI GTCGAC 1 cut(s) 2068
SapI GCTCTTC 1 cut(s) 2036
Sau96I GGNCC 7 cut(s) 85, 363, 825, 987, 1470, 1817, 2899
SchI GAGTC 3 cut(s) 213, 2195, 2765
ScrFI CCNGG 4 cut(s) 302, 1098, 1233, 2301
SduI GDGCHC 5 cut(s) 274, 802, 1654, 2048, 2957
SfcI CTRYAG 7 cut(s) 573, 2082, 2259, 2796, 2916, 2994, 3296
SfuI TTCGAA 1 cut(s) 1723
SinI GGWCC 6 cut(s) 85, 363, 825, 987, 1817, 2899
SmiMI CAYNNNNRTG 3 cut(s) 1248, 2000, 2720
SmlI CTYRAG 5 cut(s) 353, 1806, 2028, 3228, 3266
SmoI CTYRAG 5 cut(s) 353, 1806, 2028, 3228, 3266
SseBI AGGCCT 1 cut(s) 2034
SsiI CCGC 9 cut(s) 13, 74, 402, 1041, 1820, 2255, 2268, 2990, 3221
SspI AATATT 1 cut(s) 2097
SspMI CTAG 5 cut(s) 368, 380, 458, 1538, 1983
StuI AGGCCT 1 cut(s) 2034
StyD4I CCNGG 4 cut(s) 300, 1096, 1231, 2299
StyI CCWWGG 5 cut(s) 418, 1432, 2109, 2168, 2271
TaaI ACNGT 7 cut(s) 984, 1075, 1500, 1533, 2074, 2782, 2976
TaiI ACGT 2 cut(s) 878, 1737
TaqI TCGA 8 cut(s) 141, 213, 741, 1723, 2069, 2432, 2940, 3358
TaqII GACCGA 1 cut(s) 1370
TatI WGTACW 1 cut(s) 2427
TauI GCSGC 4 cut(s) 16, 1044, 2270, 3224
TscAI CASTG 7 cut(s) 989, 1387, 1932, 2341, 2424, 2818, 3330
TseFI GTSAC 3 cut(s) 1092, 1771, 3072
TseI GCWGC 6 cut(s) 542, 2789, 2792, 2795, 2804, 2918
Tsp45I GTSAC 3 cut(s) 1092, 1771, 3072
TspGWI ACGGA 1 cut(s) 880
TspRI CASTG 7 cut(s) 989, 1387, 1932, 2341, 2424, 2818, 3330
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 6 cut(s) 85, 363, 825, 987, 1817, 2899
XagI CCTNNNNNAGG 1 cut(s) 2298
XapI RAATTY 9 cut(s) 526, 582, 1108, 1725, 1849, 2023, 2323, 2386, 3372
XbaI TCTAGA 1 cut(s) 379
XceI RCATGY 1 cut(s) 2129
XcmI CCANNNNNNNNNTGG 3 cut(s) 172, 710, 835
XmiI GTMKAC 2 cut(s) 2069, 2997
XmnI GAANNNNTTC 3 cut(s) 2308, 2390, 2741
XspI CTAG 5 cut(s) 368, 380, 458, 1538, 1983
Zsp2I ATGCAT 1 cut(s) 1975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.