pycom12g16030

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002511
Physical Location & Seq
Reverse (-)
18466797 .. 18471094
4298 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g16030.11

Sequence Viewer

Length: 2280 bp
ATGGGGAATTTAAGATGTGTTGGTCGTACAGACCTTACCCTTACTGGCTCTTTTGCAGACATGATTTTGTTACCAAGTTCTGGGACAACTGGGGGTAATCACAAGGCTGATGCTTTTGTATTAACCAACCCTGGGCAACTGCATCAATATGATTATGCTACCCTCTCTGCCTTAATGTCCCAGAAAGAAAGGAATCCATCTGTATCTGCTTTGGAGTTTCCGGTGGTGATACCTACAACTAATCCAACAATGACCGTTGCCAAACTTATTAGGGTGCCCACTGGGGAGAACTTATTAAAAGCTATATCAGAGATGTCCTCAATTGGGAACCTTGGCTCAGCACAAACCCAATCAGCTGGTGCAAGATGGCCCTTGACTGGGGGTGTTCCCTGTCAACTGTCTATTTCTGAGAATAACGGTATTGAGAGACTGTACCTAGCAGGATATTCGGATGGATCTGTTCGGATATGGAATGCCACATATCCACTCTTATCTTGTATCTGCCTTGTAAAAAGCGAGGTGCATGGTATAGAAGTTGCTGGTTCAAGTGCTCCCGTAACACGACTGGATTTCTGTGCCTTCACTTTAAATCTGGCTGTTGGCAATGAATGTGGTCTGGTTCGAATTTATAACGTGAAAGGCTGTTCAGATGGGGTAAAGTTCATCTTTGTCACAGAAACCAAATATGAAGTTCACGAATCGCCTCAAGCAAAAGGACCGCGATGCAGAGCTGTATTTAGTCTGACGGATTCCCCAGTTCAATCACTTCAGTTTGTAAAGCATGGAGGTAAACTTGCTGTGGGATTTGAGTGTGGTCATGTCGCGATGCTGGATGCAAGTTCTTTTTCTGTATTGTTCTTTATAAAAGATGCATCTTTCTCTAGCTCCCCAGTCATTTCAATGATTTGGAAGGAACTTACAAATTCTCAAGGCCTCCTGAAGAGCCCAAGGGTCTCCGGAACAAAGTCGACAGTTAGTCCTGTAGAAGAAGTAATATTCATCTTAACGAAGGATGCAAATATACATGTAATTGATGGAAACACCGGTAACCTGATCATCCCTCAGCCATGGCACTTTAAAAAGGAGTCAATTGCAATTTCAATGTATGTTATAGATGGCAGATTATCTGCCTCTAAAGTGTCCGATGATAATCCACCTACAGAGGCCGCCAAGGATTCTTCAGCCAAAAATGAGCCTGTGCCAGGAAGTCTTTCAGTTGTTATAAATTCACCTGACACTGACCATGACGACTATTCAGAAAATACATACTCTGAAGAGAGATTGTTGAATTCATTCATTTTGCTTTGTTGTGTGGATTCACTGCACTTGTACTCGACAAAATCTGTGATTCAGGGAAACAATAAACCCATCCGAAAAAAGAAACATACAAGACCTTGCATTTGGACTGCAACTTTTAAGAAAGTTGAAAAGGTTTCAGGATTGGTTTTGCTGTTTCAGACTGGAGAAATTGAGATCAGATCTATTCCTGATTTAGAATTGGTGAAAGAAAGCTCCTTGCTGTCAATTCTAAGGTGGAATTGCAAGGCAAACATGGATAAAACGATGAGTTCCGATGATGCACATATTACGCTGGCAAATGGGTATGAATTGGCATTCATCTCTATGTTAGCTATTGAGAATGATTTCAGGATTCCAGAGTCTTTGCCCTGTCTCCATGATACAGTTGTTGCAGCTGCTGCAGACGCTGCACTCAGTGTCTCTGTGAATCTGAAGAAAAAACAGGGTACTGCATCTGGGATTTTAGGTGGTATTGTCAAGGGCTTTAAGGGAGGAAAAATGGTCCAGAGTGGAGATCCTGCAGCAGTTCCTAAATCAACTTTCGATCGTTTAGAGGGCACCTTTTCAAAGTCCCAACTGTCAGAACCATCCCCGCCTGTAGACCATCAAGAAGTTGTGGAGCTTAATATAGATGACATTGAAATAGATGAACCTCTATATGTAGCATCTACTTTGCCACCACATGATGTCAAAGACAAAAAGAGAGAAGGGGAATCAGAAAGGGAAAAGTTATTTGAAGGTGGTACTGGCGATACAAAGCCCAGAGTAAGAACGGCCGAAGAAATTAGAGCTAAATATAGAAAGGCTGAGGGTGCTTCATCTGCGGCTTCTCAAGCACGAAATAAGCTCATGGAGAGGCAGGAAAAACTTGAGAAAATCAGCCGGCGTACTGAAGATCTGCAGAATGGGGCTGAAGACTTCGCATCACTGGCAAACGAGCTTGTGAAGACATTGGAAAGTCGAAAATGGTGGCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000137 GO:0000139 GO:0000902 GO:0000904 GO:0002791 GO:0003674 GO:0005096 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005795 GO:0005802 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006892 GO:0006893 GO:0006996 GO:0007010 GO:0007275 GO:0007399 GO:0007409 GO:0008047 GO:0008150 GO:0009653 GO:0009987 GO:0010008 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0017016 GO:0017137 GO:0017157 GO:0019899 GO:0019900 GO:0019901 GO:0022008 GO:0022607 GO:0030029 GO:0030030 GO:0030036 GO:0030154 GO:0030182 GO:0030234 GO:0030695 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0031090 GO:0031175 GO:0031267 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0031985 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048193 GO:0048468 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050708 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051046 GO:0051049 GO:0051179 GO:0051223 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0060627 GO:0061564 GO:0065003 GO:0065007 GO:0065009 GO:0070201 GO:0071840 GO:0071944 GO:0090087 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0098876 GO:0099568 GO:0120036 GO:0120039 GO:1903530
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 Synaptobrevin

Protein Analysis

760

Amino Acids

82.62

Weight (kDa)

6.17

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 630, 863, 1223
AccB1I GGYRCC 2 cut(s) 274, 1856
AccB7I CCANNNNNTGG 1 cut(s) 80
AccI GTMKAC 2 cut(s) 968, 1899
AccII CGCG 2 cut(s) 721, 824
AccIII TCCGGA 1 cut(s) 956
AciI CCGC 4 cut(s) 719, 1167, 1892, 2123
AclWI GGATC 2 cut(s) 463, 1808
AcoI YGGCCR 1 cut(s) 2073
AcsI RAATTY 6 cut(s) 7, 624, 922, 1225, 1288, 2274
AcuI CTGAAG 7 cut(s) 752, 959, 1164, 1293, 1751, 2211, 2232
AdeI CACNNNGTG 1 cut(s) 1715
AfaI GTAC 6 cut(s) 28, 434, 1331, 1747, 2044, 2188
AfiI CCNNNNNNNGG 6 cut(s) 80, 132, 324, 377, 378, 1202
AflIII ACRYGT 1 cut(s) 1024
AgeI ACCGGT 1 cut(s) 1043
AgsI TTSAA 9 cut(s) 546, 761, 900, 1101, 1288, 1427, 1866, 1940, 2036
AhdI GACNNNNNGTC 1 cut(s) 975
AjnI CCWGG 2 cut(s) 130, 1201
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 4 cut(s) 421, 958, 1676, 1723
AlwI GGATC 2 cut(s) 463, 1808
AlwNI CAGNNNCTG 2 cut(s) 1697, 1706
Aor13HI TCCGGA 1 cut(s) 956
AoxI GGCC 4 cut(s) 368, 931, 1164, 2073
ApeKI GCWGC 5 cut(s) 1691, 1694, 1697, 1706, 1820
ApoI RAATTY 6 cut(s) 7, 624, 922, 1225, 1288, 2274
ArsI GACNNNNNNTTYG 2 cut(s) 1383, 1415
AsiGI ACCGGT 1 cut(s) 1043
Asp700I GAANNNNTTC 3 cut(s) 1210, 1292, 1643
AspS9I GGNCC 3 cut(s) 369, 716, 1801
AsuHPI GGTGA 3 cut(s) 238, 1221, 1513
AsuII TTCGAA 1 cut(s) 622
AvaII GGWCC 2 cut(s) 716, 1801
BaeGI GKGCMC 2 cut(s) 279, 1859
BaeI ACNNNNGTAYC 2 cut(s) 1671, 1704
BanI GGYRCC 2 cut(s) 274, 1856
BanII GRGCYC 1 cut(s) 947
BbsI GAAGAC 2 cut(s) 2220, 2252
Bbv12I GWGCWC 1 cut(s) 553
BbvCI CCTCAGC 2 cut(s) 1062, 2106
BbvI GCAGC 5 cut(s) 1681, 1684, 1693, 1703, 1832
BccI CCATC 9 cut(s) 205, 360, 446, 644, 1028, 1109, 1376, 1894, 1911
BceAI ACGGC 1 cut(s) 2088
BcgI CGANNNNNNTGC 2 cut(s) 429, 463
BciT130I CCWGG 2 cut(s) 132, 1203
BclI TGATCA 1 cut(s) 1053
BcoDI GTCTC 4 cut(s) 421, 958, 1676, 1723
BfaI CTAG 2 cut(s) 437, 882
BfmI CTRYAG 6 cut(s) 981, 1158, 1698, 1818, 1896, 2198
BglII AGATCT 2 cut(s) 1478, 2194
BisI GCNGC 7 cut(s) 1167, 1692, 1695, 1698, 1707, 1821, 2124
BlpI GCTNAGC 1 cut(s) 337
BlsI GCNGC 7 cut(s) 1168, 1693, 1696, 1699, 1708, 1822, 2125
Bme1390I CCNGG 2 cut(s) 132, 1203
Bme18I GGWCC 2 cut(s) 716, 1801
BmeRI GACNNNNNGTC 1 cut(s) 975
BmgT120I GGNCC 3 cut(s) 369, 716, 1801
BmiI GGNNCC 3 cut(s) 276, 329, 1858
BmrFI CCNGG 2 cut(s) 132, 1203
BmrI ACTGGG 5 cut(s) 99, 291, 387, 749, 884
BmuI ACTGGG 5 cut(s) 99, 291, 387, 749, 884
BpiI GAAGAC 2 cut(s) 2220, 2252
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 1482
Bpu10I CCTNAGC 2 cut(s) 1062, 2106
Bpu1102I GCTNAGC 1 cut(s) 337
Bpu14I TTCGAA 1 cut(s) 622
BpuEI CTTGAG 4 cut(s) 690, 912, 2115, 2189
BsaBI GATNNNNATC 1 cut(s) 1149
BsaI GGTCTC 1 cut(s) 958
BsaJI CCNNGG 6 cut(s) 130, 131, 331, 947, 1067, 1170
BsaWI WCCGGW 3 cut(s) 220, 956, 1043
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 132, 324, 377, 378, 1202
Bse118I RCCGGY 2 cut(s) 1043, 2181
Bse3DI GCAATG 1 cut(s) 610
Bse8I GATNNNNATC 1 cut(s) 1149
BseAI TCCGGA 1 cut(s) 956
BseBI CCWGG 2 cut(s) 132, 1203
BseDI CCNNGG 6 cut(s) 130, 131, 331, 947, 1067, 1170
BseGI GGATG 6 cut(s) 457, 838, 1018, 1056, 1368, 1886
BseJI GATNNNNATC 1 cut(s) 1149
BseLI CCNNNNNNNGG 6 cut(s) 80, 132, 324, 377, 378, 1202
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 5 cut(s) 351, 399, 1076, 1726, 2097
BseSI GKGCMC 2 cut(s) 279, 1859
BseX3I CGGCCG 1 cut(s) 2073
BseXI GCAGC 5 cut(s) 1681, 1684, 1693, 1703, 1832
BsgI GTGCAG 2 cut(s) 1307, 1692
Bsh1236I CGCG 2 cut(s) 721, 824
Bsh1285I CGRYCG 2 cut(s) 1846, 2076
BshFI GGCC 4 cut(s) 370, 933, 1166, 2075
BshNI GGYRCC 2 cut(s) 274, 1856
BshTI ACCGGT 1 cut(s) 1043
BsiEI CGRYCG 2 cut(s) 1846, 2076
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 4 cut(s) 221, 957, 1044, 2182
BslFI GGGAC 3 cut(s) 97, 163, 1855
BslI CCNNNNNNNGG 6 cut(s) 80, 132, 324, 377, 378, 1202
BsmAI GTCTC 4 cut(s) 421, 958, 1676, 1723
BsmFI GGGAC 3 cut(s) 97, 163, 1855
BsmI GAATGC 2 cut(s) 478, 1613
BsnI GGCC 4 cut(s) 370, 933, 1166, 2075
Bso31I GGTCTC 1 cut(s) 958
Bsp119I TTCGAA 1 cut(s) 622
Bsp1286I GDGCHC 4 cut(s) 279, 553, 947, 1859
Bsp13I TCCGGA 1 cut(s) 956
Bsp143I GATC 7 cut(s) 455, 1053, 1473, 1478, 1813, 1843, 2194
Bsp1720I GCTNAGC 1 cut(s) 337
Bsp19I CCATGG 1 cut(s) 1067
Bsp68I TCGCGA 1 cut(s) 824
BspACI CCGC 4 cut(s) 719, 1167, 1892, 2123
BspANI GGCC 4 cut(s) 370, 933, 1166, 2075
BspCNI CTCAG 5 cut(s) 350, 400, 1075, 1725, 2098
BspEI TCCGGA 1 cut(s) 956
BspFNI CGCG 2 cut(s) 721, 824
BspLI GGNNCC 3 cut(s) 276, 329, 1858
BspMAI CTGCAG 3 cut(s) 1702, 1822, 2202
BspPI GGATC 2 cut(s) 463, 1808
BspQI GCTCTTC 1 cut(s) 935
BspT104I TTCGAA 1 cut(s) 622
BspT107I GGYRCC 2 cut(s) 274, 1856
BspTNI GGTCTC 1 cut(s) 958
BsrDI GCAATG 1 cut(s) 610
BsrFI RCCGGY 2 cut(s) 1043, 2181
BssAI RCCGGY 2 cut(s) 1043, 2181
BssECI CCNNGG 6 cut(s) 130, 131, 331, 947, 1067, 1170
BssMI GATC 7 cut(s) 455, 1053, 1473, 1478, 1813, 1843, 2194
BssT1I CCWWGG 4 cut(s) 331, 947, 1067, 1170
Bst2UI CCWGG 2 cut(s) 132, 1203
Bst4CI ACNGT 7 cut(s) 256, 399, 419, 432, 973, 1684, 1878
Bst6I CTCTTC 2 cut(s) 935, 1269
BstAPI GCANNNNNTGC 2 cut(s) 1697, 1706
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 2 cut(s) 1593, 2183
BstDEI CTNAG 6 cut(s) 337, 408, 1062, 1529, 1712, 2106
BstDSI CCRYGG 1 cut(s) 1067
BstEII GGTNACC 1 cut(s) 1046
BstENI CCTNNNNNAGG 1 cut(s) 1200
BstF5I GGATG 6 cut(s) 457, 838, 1018, 1056, 1368, 1886
BstFNI CGCG 2 cut(s) 721, 824
BstKTI GATC 7 cut(s) 458, 1056, 1476, 1481, 1816, 1846, 2197
BstMAI GTCTC 4 cut(s) 421, 958, 1676, 1723
BstMBI GATC 7 cut(s) 455, 1053, 1473, 1478, 1813, 1843, 2194
BstMCI CGRYCG 2 cut(s) 1846, 2076
BstMWI GCNNNNNNNGC 7 cut(s) 1697, 1703, 1706, 2111, 2120, 2132, 2228
BstNI CCWGG 2 cut(s) 132, 1203
BstNSI RCATGY 1 cut(s) 1028
BstPI GGTNACC 1 cut(s) 1046
BstSCI CCNGG 2 cut(s) 130, 1201
BstSFI CTRYAG 6 cut(s) 981, 1158, 1698, 1818, 1896, 2198
BstSLI GKGCMC 2 cut(s) 279, 1859
BstUI CGCG 2 cut(s) 721, 824
BstV1I GCAGC 5 cut(s) 1681, 1684, 1693, 1703, 1832
BstV2I GAAGAC 2 cut(s) 2220, 2252
BstX2I RGATCY 4 cut(s) 455, 1478, 1813, 2194
BstXI CCANNNNNNTGG 1 cut(s) 356
BstYI RGATCY 4 cut(s) 455, 1478, 1813, 2194
BstZI CGGCCG 1 cut(s) 2073
BsuRI GGCC 4 cut(s) 370, 933, 1166, 2075
BtgI CCRYGG 1 cut(s) 1067
BtgZI GCGATG 2 cut(s) 736, 839
BtsCI GGATG 6 cut(s) 457, 838, 1018, 1056, 1368, 1886
BtsI GCAGTG 1 cut(s) 1319
BtsIMutI CAGTG 5 cut(s) 279, 1236, 1319, 1720, 2225
BtuMI TCGCGA 1 cut(s) 824
Cac8I GCNNGC 2 cut(s) 1593, 2183
CaiI CAGNNNCTG 2 cut(s) 1697, 1706
Cfr10I RCCGGY 2 cut(s) 1043, 2181
Cfr13I GGNCC 3 cut(s) 369, 716, 1801
CseI GACGC 1 cut(s) 1712
Csp6I GTAC 6 cut(s) 27, 433, 1330, 1746, 2043, 2187
CspAI ACCGGT 1 cut(s) 1043
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviQI GTAC 6 cut(s) 27, 433, 1330, 1746, 2043, 2187
DdeI CTNAG 6 cut(s) 337, 408, 1062, 1529, 1712, 2106
DpnI GATC 7 cut(s) 457, 1055, 1475, 1480, 1815, 1845, 2196
DpnII GATC 7 cut(s) 455, 1053, 1473, 1478, 1813, 1843, 2194
DraI TTTAAA 2 cut(s) 588, 1078
DraIII CACNNNGTG 1 cut(s) 1715
DriI GACNNNNNGTC 1 cut(s) 975
EaeI YGGCCR 1 cut(s) 2073
EagI CGGCCG 1 cut(s) 2073
Eam1104I CTCTTC 2 cut(s) 935, 1269
Eam1105I GACNNNNNGTC 1 cut(s) 975
EarI CTCTTC 2 cut(s) 935, 1269
EclXI CGGCCG 1 cut(s) 2073
Eco130I CCWWGG 4 cut(s) 331, 947, 1067, 1170
Eco147I AGGCCT 1 cut(s) 933
Eco24I GRGCYC 1 cut(s) 947
Eco31I GGTCTC 1 cut(s) 958
Eco47I GGWCC 2 cut(s) 716, 1801
Eco52I CGGCCG 1 cut(s) 2073
Eco57I CTGAAG 7 cut(s) 752, 959, 1164, 1293, 1751, 2211, 2232
Eco91I GGTNACC 1 cut(s) 1046
EcoNI CCTNNNNNAGG 1 cut(s) 1200
EcoO65I GGTNACC 1 cut(s) 1046
EcoRI GAATTC 1 cut(s) 1288
EcoRII CCWGG 2 cut(s) 130, 1201
EcoT14I CCWWGG 4 cut(s) 331, 947, 1067, 1170
EcoT22I ATGCAT 1 cut(s) 874
EcoT38I GRGCYC 1 cut(s) 947
ErhI CCWWGG 4 cut(s) 331, 947, 1067, 1170
FalI AAGNNNNNCTT 2 cut(s) 650, 682
FaqI GGGAC 3 cut(s) 97, 163, 1855
FauI CCCGC 1 cut(s) 1899
FbaI TGATCA 1 cut(s) 1053
FblI GTMKAC 2 cut(s) 968, 1899
Fnu4HI GCNGC 7 cut(s) 1167, 1692, 1695, 1698, 1707, 1821, 2124
FokI GGATG 6 cut(s) 464, 845, 1025, 1043, 1355, 1873
FriOI GRGCYC 1 cut(s) 947
Fsp4HI GCNGC 7 cut(s) 1167, 1692, 1695, 1698, 1707, 1821, 2124
FspBI CTAG 2 cut(s) 437, 882
GluI GCNGC 7 cut(s) 1167, 1692, 1695, 1698, 1707, 1821, 2124
GsuI CTGGAG 1 cut(s) 1482
HaeIII GGCC 4 cut(s) 370, 933, 1166, 2075
HapII CCGG 4 cut(s) 221, 957, 1044, 2182
HgaI GACGC 1 cut(s) 1712
HincII GTYRAC 2 cut(s) 395, 969
HindII GTYRAC 2 cut(s) 395, 969
HpaII CCGG 4 cut(s) 221, 957, 1044, 2182
HphI GGTGA 3 cut(s) 238, 1221, 1513
Hpy166II GTNNAC 5 cut(s) 395, 694, 791, 969, 1900
Hpy8I GTNNAC 5 cut(s) 395, 694, 791, 969, 1900
HpyAV CCTTC 5 cut(s) 589, 904, 1003, 2000, 2030
HpyCH4III ACNGT 7 cut(s) 256, 399, 419, 432, 973, 1684, 1878
HpyCH4IV ACGT 1 cut(s) 633
HpyF10VI GCNNNNNNNGC 7 cut(s) 1697, 1703, 1706, 2111, 2120, 2132, 2228
HpyF3I CTNAG 6 cut(s) 337, 408, 1062, 1529, 1712, 2106
HpySE526I ACGT 1 cut(s) 633
Kpn2I TCCGGA 1 cut(s) 956
KroI GCCGGC 1 cut(s) 2181
KroNI GCCGGC 1 cut(s) 2183
Ksp22I TGATCA 1 cut(s) 1053
Kzo9I GATC 7 cut(s) 455, 1053, 1473, 1478, 1813, 1843, 2194
LguI GCTCTTC 1 cut(s) 935
LmnI GCTCC 4 cut(s) 556, 890, 1517, 1918
Lsp1109I GCAGC 5 cut(s) 1681, 1684, 1693, 1703, 1832
MaeI CTAG 2 cut(s) 437, 882
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 4 cut(s) 69, 556, 670, 1046
MalI GATC 7 cut(s) 457, 1055, 1475, 1480, 1815, 1845, 2196
MboI GATC 7 cut(s) 455, 1053, 1473, 1478, 1813, 1843, 2194
MboII GAAGA 9 cut(s) 952, 998, 1170, 1286, 1744, 2090, 2204, 2225, 2257
MfeI CAATTG 2 cut(s) 321, 1089
MflI RGATCY 4 cut(s) 455, 1478, 1813, 2194
MhlI GDGCHC 4 cut(s) 279, 553, 947, 1859
MlyI GAGTC 2 cut(s) 1094, 1667
MmeI TCCRAC 1 cut(s) 269
Mph1103I ATGCAT 1 cut(s) 874
MroI TCCGGA 1 cut(s) 956
MroNI GCCGGC 1 cut(s) 2181
MroXI GAANNNNTTC 3 cut(s) 1210, 1292, 1643
MslI CAYNNNNRTG 3 cut(s) 147, 899, 1622
MspA1I CMGCKG 2 cut(s) 356, 1694
MspI CCGG 4 cut(s) 221, 957, 1044, 2182
MspR9I CCNGG 2 cut(s) 132, 1203
MunI CAATTG 2 cut(s) 321, 1089
Mva1269I GAATGC 2 cut(s) 478, 1613
MvaI CCWGG 2 cut(s) 132, 1203
MvnI CGCG 2 cut(s) 721, 824
MwoI GCNNNNNNNGC 7 cut(s) 1697, 1703, 1706, 2111, 2120, 2132, 2228
NaeI GCCGGC 1 cut(s) 2183
NcoI CCATGG 1 cut(s) 1067
NdeII GATC 7 cut(s) 455, 1053, 1473, 1478, 1813, 1843, 2194
NgoMIV GCCGGC 1 cut(s) 2181
NlaIV GGNNCC 3 cut(s) 276, 329, 1858
NmuCI GTSAC 1 cut(s) 670
NruI TCGCGA 1 cut(s) 824
NsiI ATGCAT 1 cut(s) 874
NspI RCATGY 1 cut(s) 1028
NspV TTCGAA 1 cut(s) 622
PasI CCCWGGG 1 cut(s) 131
PceI AGGCCT 1 cut(s) 933
PciI ACATGT 1 cut(s) 1024
PciSI GCTCTTC 1 cut(s) 935
PctI GAATGC 2 cut(s) 478, 1613
PdiI GCCGGC 1 cut(s) 2183
PdmI GAANNNNTTC 3 cut(s) 1210, 1292, 1643
PfeI GAWTC 9 cut(s) 193, 698, 749, 1175, 1316, 1348, 1651, 1726, 2012
PflMI CCANNNNNTGG 1 cut(s) 80
PinAI ACCGGT 1 cut(s) 1043
PkrI GCNGC 7 cut(s) 1168, 1693, 1696, 1699, 1708, 1822, 2125
Ple19I CGATCG 1 cut(s) 1846
PleI GAGTC 2 cut(s) 1093, 1666
PpsI GAGTC 2 cut(s) 1093, 1666
PscI ACATGT 1 cut(s) 1024
PsiI TTATAA 3 cut(s) 630, 863, 1223
Psp6I CCWGG 2 cut(s) 130, 1201
PspEI GGTNACC 1 cut(s) 1046
PspGI CCWGG 2 cut(s) 130, 1201
PspN4I GGNNCC 3 cut(s) 276, 329, 1858
PspPI GGNCC 3 cut(s) 369, 716, 1801
PstI CTGCAG 3 cut(s) 1702, 1822, 2202
PstNI CAGNNNCTG 2 cut(s) 1697, 1706
PsuI RGATCY 4 cut(s) 455, 1478, 1813, 2194
PvuI CGATCG 1 cut(s) 1846
PvuII CAGCTG 2 cut(s) 356, 1694
RruI TCGCGA 1 cut(s) 824
RsaI GTAC 6 cut(s) 28, 434, 1331, 1747, 2044, 2188
RsaNI GTAC 6 cut(s) 27, 433, 1330, 1746, 2043, 2187
RseI CAYNNNNRTG 3 cut(s) 147, 899, 1622
SalI GTCGAC 1 cut(s) 967
SapI GCTCTTC 1 cut(s) 935
SatI GCNGC 7 cut(s) 1167, 1692, 1695, 1698, 1707, 1821, 2124
Sau3AI GATC 7 cut(s) 455, 1053, 1473, 1478, 1813, 1843, 2194
Sau96I GGNCC 3 cut(s) 369, 716, 1801
SchI GAGTC 2 cut(s) 1094, 1667
ScrFI CCNGG 2 cut(s) 132, 1203
SduI GDGCHC 4 cut(s) 279, 553, 947, 1859
SfcI CTRYAG 6 cut(s) 981, 1158, 1698, 1818, 1896, 2198
SfuI TTCGAA 1 cut(s) 622
SinI GGWCC 2 cut(s) 716, 1801
SmiMI CAYNNNNRTG 3 cut(s) 147, 899, 1622
SmlI CTYRAG 4 cut(s) 705, 927, 2130, 2168
SmoI CTYRAG 4 cut(s) 705, 927, 2130, 2168
SseBI AGGCCT 1 cut(s) 933
SsiI CCGC 4 cut(s) 719, 1167, 1892, 2123
SspI AATATT 1 cut(s) 996
SspMI CTAG 2 cut(s) 437, 882
StuI AGGCCT 1 cut(s) 933
StyD4I CCNGG 2 cut(s) 130, 1201
StyI CCWWGG 4 cut(s) 331, 947, 1067, 1170
TaaI ACNGT 7 cut(s) 256, 399, 419, 432, 973, 1684, 1878
TaiI ACGT 1 cut(s) 636
TaqI TCGA 5 cut(s) 622, 968, 1334, 1842, 2260
TatI WGTACW 1 cut(s) 1329
TauI GCSGC 2 cut(s) 1169, 2126
TfiI GAWTC 9 cut(s) 193, 698, 749, 1175, 1316, 1348, 1651, 1726, 2012
TscAI CASTG 5 cut(s) 286, 1243, 1326, 1720, 2232
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 5 cut(s) 1691, 1694, 1697, 1706, 1820
Tsp45I GTSAC 1 cut(s) 670
TspGWI ACGGA 1 cut(s) 761
TspRI CASTG 5 cut(s) 286, 1243, 1326, 1720, 2232
Van91I CCANNNNNTGG 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 716, 1801
XagI CCTNNNNNAGG 1 cut(s) 1200
XapI RAATTY 6 cut(s) 7, 624, 922, 1225, 1288, 2274
XceI RCATGY 1 cut(s) 1028
XmiI GTMKAC 2 cut(s) 968, 1899
XmnI GAANNNNTTC 3 cut(s) 1210, 1292, 1643
XspI CTAG 2 cut(s) 437, 882
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.