Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002511

Physical Location & Seq

Genomic Coordinates Forward (+)

7559529 .. 7567649

Gene Structure 8121 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00286580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3384 bp

ATGAATCGTACGGTCGGTGAGTCCTCCTCCGCCCTCGCATCACCGTCCACCTCACACTCAATTCGCGCCTCACCGTCGTCGTCGTCCGAAACGTTTCAGAAACCGAAACCAAACGCCATTCTTGTTTTTCCTTCCCACCTTTCAAATCAGACCAAGAAACACGAAACAGCAAGGGTGTCACAAGCTGATTTGGATCCAAGAATTGTTTTTCACTATGGGATTCCATCGACTGCATCGATTCTCGCACTTGATCGCACGCAGAGCTTGCTGGCAATTGGAACCCTTTTAGTTGAAGCGTTTATTGATCAAATAGCAGAGTTCTTGAATGATGAGTTCTTAGAAAATCAAGGTTTTTTAGCCAGTGTCTCAAGTGAAAATGAAATTCAGGTTTGGGATTTGGAACAGAAGCAAATAGCTGGTTCTTTACAGTGGGAATGCAATATAACTGCCTTCTCTGTTATTTATGGCACCAATTACATGTATATTGGAAGCGAATATGCAATGGTGTCTATGGTGAAGTATGATGCTGAAGATCGAAAAATCAATTTACTGCCATATTATATTACTGCAAACTTCATAGCTGAAGCAGCTGGAATGTCATTGCCTGATAATCTTTCTGTTGTTGGAGCTGAGCTGGAGGGGCAAGAACATGAGGGACTGCTGATTGCATATGAGAATGGTCTGATTGTTCTCTGGGATGCTTTAGAAGATCGAGTTGTGCTTGTAAGAGGTTCCAAGGACCTGCAAGTGAAGGAAGAAACAGTCCATAATTCTTTTGAAGGCACAAGAACTGAGCTGACTGATGCTACATCAGACAGCAAACAGGTGGAGAAAGAGATAAGCTCTCTTTGTTGGGTATGCGATAATGGGTCAAGTCTTGCTGTTGGCTATGTAGATGGTGATATAATGTTTTGGGATATATCAACTGCTGAGTCTACCAAGGATCATAGATCTAAAAAGTTACCTAACAATGTTGCGAAGCTAGAGTTATCATCAGATGACAAGAGACTCCCTGTCATAGTTTTACACTGGTCTGCAAATAGGTTAAGTCATCATCCTAGGGGCCAACTCTTTGTTTATGGAGGTGAAGGAATTGGGTCTGAAGAAGTTCTCACGGTTCTGAGCGTGGATTGGTCTTCAGGAATAGAAAGTCTGAAATGCATAGGTCGTGTGGACCTTACACTTGTTGGTTCATTTGCAGATATGGTTTTACTTCCAGCTGCTGGTGCAATGGAGAACGGTGACACATTGCTATTCACTTTGACAAATCAGGGACAACTGCATGTCTATGACAAGGCTTGCTGGTCTGCCTTAATGTCTCACCAAAAGAAAAGGAGTGATGGGACTGCAGTGCAGTATCCTATGTTTATACCTACTACTGAACCGTGTATGACAGTGGCAAAGCTTGCTTTGGTGGATAGAGATGGGAAATACTCCTCAGCTTTGTCCAAGGTAGTGTTCTTTTGTGACACAATAAACTCTAGTTACATTGCATCTTGGTACTATCATCATCCATCACTTAGCCAACTTTCTGATGCTGAAAATTTTCATGTCGAGAGACTATATGTAGCAGGATATCAAGATGGATCTGTTCGAATATGGGATGCCACCTATCCAACTCCGTCGCTTATATATGTTATAGGTCCTGAGGTAAAAGGCATCAGATCTACAGGTACAAATGGAACAGTCTCAGCATTGGAATTTTGTTCTCTTACTTTAAAATTAGCTATTGGTGATGTGTGTGGTCTGGTTCGTCTATATAAACTAATAGGGAGTTCAGACGAGACAGAATTGCACGTTGTGACAAAAACAGAGAAGGAAGTTTATACTTCACAACAAGGTAATGGGCCTCGGTGCACAGCTGTGCTTTCTATTCTTGATTCTCCTATTTCCATACTGCAATATACAAATTTTGGAGCAAGACTCACTGTGGGATATGAATGTGGCCGGGTTGCAATGCTTGATATTAGTACATCGTCAGTTTTGTTTCTTACAGACAGTGTCTCCAACTCAAGTTCCCCAGATTCTGAATCCAAAACTTTAAGTGATTCTGGAAATGGGTTGGCAGTCCCAAAGGACTCTGAATCCAAGAATGTGGCTGACCCTGAACATGGAATGAGTGTAAAAGATCCTGATTCCCAGACTTTGAGTGATTCTGGAAATGAGTTGGCAATCCCAAAAGACTCTGAATGCAAGAATGTGACTGATCCTGAACATGGAAAGAGTTTAAAAGATCCCGAATCCAAGACTTTGGGTGATTCTGAAAATGGTTTGGCGTTCGTTATGACCAGAAATGCACATTTTGTTATTTTAGATAGTGCCACTGGCAATATGATCAGGTCTTGGCCCATGCATCCAAAAATGGATTCTACTGCGATTTCGATGTACATTATAGAGGATGGTGATGTTTTATTTGATTTGTCGAGTGCAAAAAAAGATTCTTTAGACTTACCTCAGAAAGGCGTAGCTAAACATGATGACGTACCACCTAATGCTGACTCTGGAGGGACACAGCTTGTAGTTGACCAGGATACCTCCACCAAAACTGCATATTTTTTGCAGAGATTGGTGAACATATGTGTTTTACTTTGTTTTGACTACACATTGCACTTATGTTCATTGAAGTCGCTGCTTGAGGGTGACAGTAACTGCATTCAGAAGGTGAATCTTGTAAAACCGTGTTGTTGGACTACAATCTTCAAAAAAGATGGAAAAGACAGTGGATTGGTTATACTAAATCAGACAGGAGTCTTTGAGATCAGGATTTCGGAGTCCTTCCCTTGTCTTCATGATAAAGTGCTTGCAGCTGCTACAGATGTCATTGCCGATTTATCTCAAAGACAGAAACAGAGTGCTGCCCATAGGATTTTAGGAGGTATCATTAAGGGCTTAAAAACTTGTAAAACGGATCAAAACATGGACCCGACTGGAAATCATGAAAAATATTGTGCGAATTTGGAGAGCTTATTTTCAAATCCCCCATTCTTAAAACCTTCCACAGATATGAAGGATGGCCAAGAACCCGTGGTGCTTAACCTAGATGATATTGTAATTGATGGCCCTATTACTGTCTTGCCTAAAGATCCAAACAAGAAGAATGAAAAGAAAGACAAGGGATCAGACAAGAAAAAGTTATTTGAAGGCGCAACTAGTGGTACAAAGCCAAAAATGAGGACAGCTGCCGAAATCAAGGCTAAATATAGAGAAACTGGGGATGTCTCTGCCGCAGCTGCACGTGCAAGAGATATGCTTGCCGAACGTGGGGAAAAACTTGAGAAACTCAGAGAAAACACAGAAGAACTATCGAGTGGAGCTCAAGACTTCGCATCAATGGCAAAGGAACTCGCCAAGAGAATGGAGAATCGTAAATGGTGGCACATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0002791 GO:0002792 GO:0002793 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0008047 GO:0008150 GO:0010646 GO:0010648 GO:0010817 GO:0016020 GO:0017016 GO:0017137 GO:0017157 GO:0019899 GO:0019905 GO:0023051 GO:0023057 GO:0030234 GO:0030695 GO:0031267 GO:0032879 GO:0032880 GO:0033500 GO:0042592 GO:0042593 GO:0043085 GO:0043087 GO:0043547 GO:0044093 GO:0044424 GO:0044464 GO:0046676 GO:0046883 GO:0046888 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048878 GO:0050708 GO:0050709 GO:0050714 GO:0050789 GO:0050790 GO:0050794 GO:0050796 GO:0051020 GO:0051046 GO:0051047 GO:0051048 GO:0051049 GO:0051050 GO:0051051 GO:0051222 GO:0051223 GO:0051224 GO:0051336 GO:0051345 GO:0060589 GO:0060627 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0071944 GO:0090087 GO:0090276 GO:0090278 GO:0098772 GO:1903530 GO:1903531 GO:1903532 GO:1904950 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANAPC4_WD40 Synaptobrevin

Protein Analysis

1127

Amino Acids

123.5

Weight (kDa)

5.18

Isoelectric Point (pI)

34.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Synaptobrevin	PF00957	1082 - 1124	1.9e-06	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002511)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05570 AT5G05570
fragaria_vesca	FvH4_6g11290 FvH4_6g11290 FvH4_6g11290 FvH4_7g24600 FvH4_7g24600
malus_domestica	MD01G1154200.v1.1 MD04G1155600.v1.1 MD07G1223200.v1.1 MD12G1168800.v1.1
prunus_persica	Prupe.2G251300_v2.0.a1 Prupe.6G279100_v2.0.a1 Prupe.6G279100_v2.0.a1
pyrus_communis	pycom01g17150 pycom04g14030 pycom12g16030
rosa_chinensis	RchiOBHm_Chr1g0370431 RchiOBHm_Chr3g0462831
rosa_laevigata	RLG00000024829 RLG00000027044
rosa_multiflora	Rmu_sc0000766.1_g000011 Rmu_sc0005656.1_g000008 Rmu_sc0035228.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286580 Rroxscaffold_6G00417170
rosa_rugosa	Rorug01G0356400 Rorug01G0356500 Rorug01G0356600 Rorug03G0059500 Rorug03G0059500
rosa_samantha	Rh1AG364900 Rh1BG328100 Rh1CG341100 Rh1DG358400 Rh3AG117600 Rh3BG121400 Rh3CG124300 Rh3DG123000
rosa_wichuraiana	Rw1G032160 Rw3G009970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	750
AccB1I	GGYRCC	1 cut(s)	467
AccB7I	CCANNNNNTGG	1 cut(s)	1223
AccI	GTMKAC	1 cut(s)	935
AccII	CGCG	1 cut(s)	66
AciI	CCGC	2 cut(s)	30, 3225
AclI	AACGTT	1 cut(s)	92
AcoI	YGGCCR	2 cut(s)	1945, 3013
AcsI	RAATTY	5 cut(s)	381, 1543, 1700, 1909, 2953
AcuI	CTGAAG	4 cut(s)	549, 603, 1122, 1122
AcvI	CACGTG	1 cut(s)	3236
AdeI	CACNNNGTG	1 cut(s)	1801
AfaI	GTAC	7 cut(s)	10, 1502, 1675, 1972, 2387, 2484, 3157
AfiI	CCNNNNNNNGG	5 cut(s)	1223, 2111, 2216, 2459, 3261
AflIII	ACRYGT	1 cut(s)	477
AgsI	TTSAA	8 cut(s)	144, 293, 325, 779, 2623, 2701, 2973, 3140
AhdI	GACNNNNNGTC	1 cut(s)	1013
AhlI	ACTAGT	1 cut(s)	3149
AjnI	CCWGG	1 cut(s)	2526
AjuI	GAANNNNNNNTTGG	4 cut(s)	1442, 1474, 2350, 2382
AleI	CACNNNNGTG	1 cut(s)	1862
Alw21I	GWGCWC	2 cut(s)	1859, 3316
Alw26I	GTCTC	8 cut(s)	370, 1000, 1323, 1552, 1693, 1778, 2008, 3223
Alw44I	GTGCAC	1 cut(s)	1855
AlwNI	CAGNNNCTG	3 cut(s)	1223, 2027, 2649
AoxI	GGCC	6 cut(s)	1063, 1847, 1945, 2345, 3013, 3058
ApaLI	GTGCAC	1 cut(s)	1855
ApeKI	GCWGC	9 cut(s)	587, 1220, 2629, 2804, 2807, 2855, 3179, 3227, 3230
ApoI	RAATTY	5 cut(s)	381, 1543, 1700, 1909, 2953
Asp700I	GAANNNNTTC	3 cut(s)	93, 1107, 1545
AspA2I	CCTAGG	1 cut(s)	1058
AspLEI	GCGC	2 cut(s)	68, 3146
AspS9I	GGNCC	8 cut(s)	739, 1063, 1174, 1643, 1847, 2346, 2920, 3059
AsuC2I	CCSGG	1 cut(s)	1949
AsuII	TTCGAA	1 cut(s)	1594
AvaII	GGWCC	4 cut(s)	739, 1174, 1643, 2920
AvrII	CCTAGG	1 cut(s)	1058
AxyI	CCTNAGG	1 cut(s)	1647
BaeGI	GKGCMC	1 cut(s)	1859
BalI	TGGCCA	1 cut(s)	3015
BamHI	GGATCC	1 cut(s)	193
BanI	GGYRCC	1 cut(s)	467
BanII	GRGCYC	1 cut(s)	3316
BbrPI	CACGTG	1 cut(s)	3236
BbsI	GAAGAC	2 cut(s)	1128, 2777
Bbv12I	GWGCWC	2 cut(s)	1859, 3316
BbvCI	CCTCAGC	1 cut(s)	1438
BbvI	GCAGC	9 cut(s)	599, 1207, 2616, 2794, 2816, 2842, 3166, 3217, 3239
BciT130I	CCWGG	1 cut(s)	2528
BciVI	GTATCC	2 cut(s)	1368, 2524
BclI	TGATCA	2 cut(s)	304, 2334
BcnI	CCSGG	1 cut(s)	1949
BcoDI	GTCTC	8 cut(s)	370, 1000, 1323, 1552, 1693, 1778, 2008, 3223
BcuI	ACTAGT	1 cut(s)	3149
BfaI	CTAG	5 cut(s)	983, 1059, 1482, 3038, 3150
BfmI	CTRYAG	3 cut(s)	1347, 1668, 2811
BfuAI	ACCTGC	1 cut(s)	750
BfuI	GTATCC	2 cut(s)	1368, 2524
BglII	AGATCT	2 cut(s)	950, 1664
BlnI	CCTAGG	1 cut(s)	1058
BlpI	GCTNAGC	1 cut(s)	630
Bme1390I	CCNGG	2 cut(s)	1949, 2528
Bme18I	GGWCC	4 cut(s)	739, 1174, 1643, 2920
BmeRI	GACNNNNNGTC	1 cut(s)	1013
BmgT120I	GGNCC	8 cut(s)	739, 1063, 1174, 1643, 1847, 2346, 2920, 3059
BmiI	GGNNCC	6 cut(s)	195, 280, 469, 733, 1064, 2922
BmrFI	CCNGG	2 cut(s)	1949, 2528
BmrI	ACTGGG	1 cut(s)	3219
BmuI	ACTGGG	1 cut(s)	3219
BoxI	GACNNNNGTC	1 cut(s)	2747
BpiI	GAAGAC	2 cut(s)	1128, 2777
BplI	GAGNNNNNCTC	8 cut(s)	11, 43, 827, 859, 1908, 1940, 3298, 3330
BpmI	CTGGAG	2 cut(s)	656, 2523
Bpu10I	CCTNAGC	1 cut(s)	1438
Bpu1102I	GCTNAGC	1 cut(s)	630
Bpu14I	TTCGAA	1 cut(s)	1594
BpuEI	CTTGAG	5 cut(s)	352, 1996, 2654, 3293, 3300
BpuMI	CCSGG	1 cut(s)	1949
Bsa29I	ATCGAT	1 cut(s)	236
BsaAI	YACGTR	1 cut(s)	3236
BsaBI	GATNNNNATC	1 cut(s)	192
BsaJI	CCNNGG	6 cut(s)	735, 939, 1058, 1449, 1850, 3024
BsaXI	ACNNNNNCTCC	2 cut(s)	1988, 2018
Bsc4I	CCNNNNNNNGG	5 cut(s)	1223, 2111, 2216, 2459, 3261
Bse1I	ACTGG	5 cut(s)	360, 1034, 2329, 2932, 3214
Bse21I	CCTNAGG	1 cut(s)	1647
Bse3DI	GCAATG	8 cut(s)	507, 599, 1236, 1247, 1488, 1962, 2603, 2820
Bse8I	GATNNNNATC	1 cut(s)	192
BseBI	CCWGG	1 cut(s)	2528
BseCI	ATCGAT	1 cut(s)	236
BseDI	CCNNGG	6 cut(s)	735, 939, 1058, 1449, 1850, 3024
BseGI	GGATG	8 cut(s)	703, 1054, 1510, 1609, 2353, 2404, 3016, 3220
BseJI	GATNNNNATC	1 cut(s)	192
BseLI	CCNNNNNNNGG	5 cut(s)	1223, 2111, 2216, 2459, 3261
BseMI	GCAATG	8 cut(s)	507, 599, 1236, 1247, 1488, 1962, 2603, 2820
BseMII	CTCAG	9 cut(s)	621, 783, 921, 1112, 1452, 1638, 1704, 2468, 3295
BseNI	ACTGG	5 cut(s)	360, 1034, 2329, 2932, 3214
BseRI	GAGGAG	2 cut(s)	16, 1426
BseSI	GKGCMC	1 cut(s)	1859
BseXI	GCAGC	9 cut(s)	599, 1207, 2616, 2794, 2816, 2842, 3166, 3217, 3239
BsgI	GTGCAG	2 cut(s)	1373, 3216
Bsh1236I	CGCG	1 cut(s)	66
Bsh1285I	CGRYCG	1 cut(s)	15
BshFI	GGCC	6 cut(s)	1065, 1849, 1947, 2347, 3015, 3060
BshNI	GGYRCC	1 cut(s)	467
BshVI	ATCGAT	1 cut(s)	236
BsiEI	CGRYCG	1 cut(s)	15
BsiHKAI	GWGCWC	2 cut(s)	1859, 3316
BsiSI	CCGG	1 cut(s)	1948
BsiWI	CGTACG	1 cut(s)	8
BslFI	GGGAC	5 cut(s)	669, 1287, 1357, 2054, 2521
BslI	CCNNNNNNNGG	5 cut(s)	1223, 2111, 2216, 2459, 3261
BsmAI	GTCTC	8 cut(s)	370, 1000, 1323, 1552, 1693, 1778, 2008, 3223
BsmFI	GGGAC	5 cut(s)	669, 1287, 1357, 2054, 2521
BsmI	GAATGC	3 cut(s)	440, 2195, 2652
BsnI	GGCC	6 cut(s)	1065, 1849, 1947, 2347, 3015, 3060
Bsp119I	TTCGAA	1 cut(s)	1594
Bsp1286I	GDGCHC	2 cut(s)	1859, 3316
Bsp1407I	TGTACA	1 cut(s)	2385
Bsp1720I	GCTNAGC	1 cut(s)	630
BspACI	CCGC	2 cut(s)	30, 3225
BspANI	GGCC	6 cut(s)	1065, 1849, 1947, 2347, 3015, 3060
BspCNI	CTCAG	9 cut(s)	622, 784, 922, 1113, 1451, 1639, 1703, 2467, 3294
BspDI	ATCGAT	1 cut(s)	236
BspFNI	CGCG	1 cut(s)	66
BspHI	TCATGA	2 cut(s)	2788, 2935
BspLI	GGNNCC	6 cut(s)	195, 280, 469, 733, 1064, 2922
BspMAI	CTGCAG	1 cut(s)	1351
BspMI	ACCTGC	1 cut(s)	750
BspT104I	TTCGAA	1 cut(s)	1594
BspT107I	GGYRCC	1 cut(s)	467
BsrDI	GCAATG	8 cut(s)	507, 599, 1236, 1247, 1488, 1962, 2603, 2820
BsrGI	TGTACA	1 cut(s)	2385
BsrI	ACTGG	5 cut(s)	360, 1034, 2329, 2932, 3214
BssECI	CCNNGG	6 cut(s)	735, 939, 1058, 1449, 1850, 3024
BssT1I	CCWWGG	4 cut(s)	735, 939, 1058, 1449
Bst2UI	CCWGG	1 cut(s)	2528
BstAPI	GCANNNNNTGC	2 cut(s)	265, 1406
BstAUI	TGTACA	1 cut(s)	2385
BstBAI	YACGTR	1 cut(s)	3236
BstBI	TTCGAA	1 cut(s)	1594
BstC8I	GCNNGC	7 cut(s)	257, 266, 270, 1300, 1407, 2802, 3252
BstDSI	CCRYGG	1 cut(s)	3024
BstENI	CCTNNNNNAGG	1 cut(s)	2457
BstF5I	GGATG	8 cut(s)	703, 1054, 1510, 1609, 2353, 2404, 3016, 3220
BstFNI	CGCG	1 cut(s)	66
BstHHI	GCGC	2 cut(s)	68, 3146
BstMAI	GTCTC	8 cut(s)	370, 1000, 1323, 1552, 1693, 1778, 2008, 3223
BstMCI	CGRYCG	1 cut(s)	15
BstMWI	GCNNNNNNNGC	9 cut(s)	261, 265, 587, 640, 1226, 1406, 3230, 3236, 3332
BstNI	CCWGG	1 cut(s)	2528
BstNSI	RCATGY	2 cut(s)	481, 1286
BstPAI	GACNNNNGTC	1 cut(s)	2747
BstSCI	CCNGG	2 cut(s)	1947, 2526
BstSFI	CTRYAG	3 cut(s)	1347, 1668, 2811
BstSLI	GKGCMC	1 cut(s)	1859
BstUI	CGCG	1 cut(s)	66
BstV1I	GCAGC	9 cut(s)	599, 1207, 2616, 2794, 2816, 2842, 3166, 3217, 3239
BstV2I	GAAGAC	2 cut(s)	1128, 2777
BstX2I	RGATCY	7 cut(s)	193, 950, 1586, 1664, 2128, 2233, 3082
BstXI	CCANNNNNNTGG	3 cut(s)	2095, 2251, 3355
BstYI	RGATCY	7 cut(s)	193, 950, 1586, 1664, 2128, 2233, 3082
Bsu15I	ATCGAT	1 cut(s)	236
Bsu36I	CCTNAGG	1 cut(s)	1647
BsuI	GTATCC	2 cut(s)	1368, 2524
BsuRI	GGCC	6 cut(s)	1065, 1849, 1947, 2347, 3015, 3060
BsuTUI	ATCGAT	1 cut(s)	236
BtgI	CCRYGG	1 cut(s)	3024
BtsCI	GGATG	8 cut(s)	703, 1054, 1510, 1609, 2353, 2404, 3016, 3220
BtsI	GCAGTG	1 cut(s)	1356
BtsIMutI	CAGTG	9 cut(s)	367, 434, 1027, 1356, 1401, 1926, 2005, 2322, 2725
BveI	ACCTGC	1 cut(s)	750
Cac8I	GCNNGC	7 cut(s)	257, 266, 270, 1300, 1407, 2802, 3252
CaiI	CAGNNNCTG	3 cut(s)	1223, 2027, 2649
CciI	TCATGA	2 cut(s)	2788, 2935
CfoI	GCGC	2 cut(s)	68, 3146
Cfr13I	GGNCC	8 cut(s)	739, 1063, 1174, 1643, 1847, 2346, 2920, 3059
ClaI	ATCGAT	1 cut(s)	236
Csp6I	GTAC	7 cut(s)	9, 1501, 1674, 1971, 2386, 2483, 3156
CviQI	GTAC	7 cut(s)	9, 1501, 1674, 1971, 2386, 2483, 3156
DraI	TTTAAA	2 cut(s)	1719, 2229
DraIII	CACNNNGTG	1 cut(s)	1801
DriI	GACNNNNNGTC	1 cut(s)	1013
EaeI	YGGCCR	2 cut(s)	1945, 3013
Eam1105I	GACNNNNNGTC	1 cut(s)	1013
EciI	GGCGGA	1 cut(s)	19
Ecl136II	GAGCTC	1 cut(s)	3314
Eco130I	CCWWGG	4 cut(s)	735, 939, 1058, 1449
Eco24I	GRGCYC	1 cut(s)	3316
Eco32I	GATATC	1 cut(s)	1577
Eco47I	GGWCC	4 cut(s)	739, 1174, 1643, 2920
Eco53kI	GAGCTC	1 cut(s)	3314
Eco57I	CTGAAG	4 cut(s)	549, 603, 1122, 1122
Eco72I	CACGTG	1 cut(s)	3236
Eco81I	CCTNAGG	1 cut(s)	1647
EcoICRI	GAGCTC	1 cut(s)	3314
EcoNI	CCTNNNNNAGG	1 cut(s)	2457
EcoO109I	RGGNCCY	2 cut(s)	739, 1643
EcoRII	CCWGG	1 cut(s)	2526
EcoRV	GATATC	1 cut(s)	1577
EcoT14I	CCWWGG	4 cut(s)	735, 939, 1058, 1449
EcoT22I	ATGCAT	2 cut(s)	1163, 2355
EcoT38I	GRGCYC	1 cut(s)	3316
ErhI	CCWWGG	4 cut(s)	735, 939, 1058, 1449
FaqI	GGGAC	5 cut(s)	669, 1287, 1357, 2054, 2521
FauNDI	CATATG	3 cut(s)	670, 2576, 3380
FbaI	TGATCA	2 cut(s)	304, 2334
FblI	GTMKAC	1 cut(s)	935
FokI	GGATG	8 cut(s)	710, 1041, 1497, 1616, 2340, 2411, 3023, 3227
FriOI	GRGCYC	1 cut(s)	3316
FspBI	CTAG	5 cut(s)	983, 1059, 1482, 3038, 3150
GlaI	GCGC	2 cut(s)	67, 3145
GsuI	CTGGAG	2 cut(s)	656, 2523
HaeIII	GGCC	6 cut(s)	1065, 1849, 1947, 2347, 3015, 3060
HapII	CCGG	1 cut(s)	1948
HhaI	GCGC	2 cut(s)	68, 3146
Hin6I	GCGC	2 cut(s)	66, 3144
HinP1I	GCGC	2 cut(s)	66, 3144
HincII	GTYRAC	1 cut(s)	2524
HindII	GTYRAC	1 cut(s)	2524
HindIII	AAGCTT	1 cut(s)	1403
HpaII	CCGG	1 cut(s)	1948
Hpy166II	GTNNAC	6 cut(s)	48, 936, 1174, 1857, 2524, 2572
Hpy8I	GTNNAC	6 cut(s)	48, 936, 1174, 1857, 2524, 2572
Hpy99I	CGWCG	4 cut(s)	79, 82, 85, 1627
HpyCH4IV	ACGT	5 cut(s)	92, 1797, 2481, 3235, 3259
HpyF10VI	GCNNNNNNNGC	9 cut(s)	261, 265, 587, 640, 1226, 1406, 3230, 3236, 3332
HpySE526I	ACGT	5 cut(s)	92, 1797, 2481, 3235, 3259
HspAI	GCGC	2 cut(s)	66, 3144
Ksp22I	TGATCA	2 cut(s)	304, 2334
LmnI	GCTCC	3 cut(s)	626, 1916, 3311
Lsp1109I	GCAGC	9 cut(s)	599, 1207, 2616, 2794, 2816, 2842, 3166, 3217, 3239
MaeI	CTAG	5 cut(s)	983, 1059, 1482, 3038, 3150
MaeII	ACGT	5 cut(s)	92, 1797, 2481, 3235, 3259
MaeIII	GTNAC	9 cut(s)	177, 960, 1241, 1466, 1484, 1801, 2200, 2639, 2645
MboII	GAAGA	9 cut(s)	542, 719, 767, 1115, 1128, 2689, 2777, 3106, 3308
MfeI	CAATTG	1 cut(s)	273
MflI	RGATCY	7 cut(s)	193, 950, 1586, 1664, 2128, 2233, 3082
MhlI	GDGCHC	2 cut(s)	1859, 3316
MlsI	TGGCCA	1 cut(s)	3015
MluNI	TGGCCA	1 cut(s)	3015
MlyI	GAGTC	9 cut(s)	29, 941, 1002, 1917, 2072, 2177, 2492, 2757, 2780
MmeI	TCCRAC	4 cut(s)	604, 1640, 2031, 2666
Mox20I	TGGCCA	1 cut(s)	3015
Mph1103I	ATGCAT	2 cut(s)	1163, 2355
MroXI	GAANNNNTTC	3 cut(s)	93, 1107, 1545
MscI	TGGCCA	1 cut(s)	3015
MseI	TTAA	9 cut(s)	1046, 1313, 1718, 2042, 2228, 2883, 2891, 2987, 3033
MslI	CAYNNNNRTG	3 cut(s)	1287, 1862, 3002
Msp20I	TGGCCA	1 cut(s)	3015
MspA1I	CMGCKG	6 cut(s)	590, 1220, 1862, 2807, 3179, 3230
MspI	CCGG	1 cut(s)	1948
MspR9I	CCNGG	2 cut(s)	1949, 2528
MunI	CAATTG	1 cut(s)	273
Mva1269I	GAATGC	3 cut(s)	440, 2195, 2652
MvaI	CCWGG	1 cut(s)	2528
MvnI	CGCG	1 cut(s)	66
MwoI	GCNNNNNNNGC	9 cut(s)	261, 265, 587, 640, 1226, 1406, 3230, 3236, 3332
NciI	CCSGG	1 cut(s)	1949
NdeI	CATATG	3 cut(s)	670, 2576, 3380
NlaIV	GGNNCC	6 cut(s)	195, 280, 469, 733, 1064, 2922
NmuCI	GTSAC	6 cut(s)	177, 1241, 1466, 1801, 2200, 2639
NsiI	ATGCAT	2 cut(s)	1163, 2355
NspI	RCATGY	2 cut(s)	481, 1286
NspV	TTCGAA	1 cut(s)	1594
OliI	CACNNNNGTG	1 cut(s)	1862
PagI	TCATGA	2 cut(s)	2788, 2935
PciI	ACATGT	1 cut(s)	477
PcsI	WCGNNNNNNNCGW	2 cut(s)	89, 233
PctI	GAATGC	3 cut(s)	440, 2195, 2652
PdmI	GAANNNNTTC	3 cut(s)	93, 1107, 1545
Pfl23II	CGTACG	1 cut(s)	8
PflFI	GACNNNGTC	1 cut(s)	2000
PflMI	CCANNNNNTGG	1 cut(s)	1223
PleI	GAGTC	9 cut(s)	28, 940, 1002, 1917, 2072, 2177, 2492, 2756, 2779
PmaCI	CACGTG	1 cut(s)	3236
PmlI	CACGTG	1 cut(s)	3236
PpsI	GAGTC	9 cut(s)	28, 940, 1002, 1917, 2072, 2177, 2492, 2756, 2779
Ppu21I	YACGTR	1 cut(s)	3236
PpuMI	RGGWCCY	2 cut(s)	739, 1643
PscI	ACATGT	1 cut(s)	477
PshAI	GACNNNNGTC	1 cut(s)	2747
Psp124BI	GAGCTC	1 cut(s)	3316
Psp1406I	AACGTT	1 cut(s)	92
Psp5II	RGGWCCY	2 cut(s)	739, 1643
Psp6I	CCWGG	1 cut(s)	2526
PspCI	CACGTG	1 cut(s)	3236
PspGI	CCWGG	1 cut(s)	2526
PspLI	CGTACG	1 cut(s)	8
PspN4I	GGNNCC	6 cut(s)	195, 280, 469, 733, 1064, 2922
PspPI	GGNCC	8 cut(s)	739, 1063, 1174, 1643, 1847, 2346, 2920, 3059
PspPPI	RGGWCCY	2 cut(s)	739, 1643
PstI	CTGCAG	1 cut(s)	1351
PstNI	CAGNNNCTG	3 cut(s)	1223, 2027, 2649
PsuI	RGATCY	7 cut(s)	193, 950, 1586, 1664, 2128, 2233, 3082
PsyI	GACNNNGTC	1 cut(s)	2000
PvuII	CAGCTG	6 cut(s)	590, 1220, 1862, 2807, 3179, 3230
RsaI	GTAC	7 cut(s)	10, 1502, 1675, 1972, 2387, 2484, 3157
RsaNI	GTAC	7 cut(s)	9, 1501, 1674, 1971, 2386, 2483, 3156
RseI	CAYNNNNRTG	3 cut(s)	1287, 1862, 3002
SacI	GAGCTC	1 cut(s)	3316
SaqAI	TTAA	9 cut(s)	1046, 1313, 1718, 2042, 2228, 2883, 2891, 2987, 3033
Sau96I	GGNCC	8 cut(s)	739, 1063, 1174, 1643, 1847, 2346, 2920, 3059
SchI	GAGTC	9 cut(s)	29, 941, 1002, 1917, 2072, 2177, 2492, 2757, 2780
ScrFI	CCNGG	2 cut(s)	1949, 2528
SduI	GDGCHC	2 cut(s)	1859, 3316
SfcI	CTRYAG	3 cut(s)	1347, 1668, 2811
SfuI	TTCGAA	1 cut(s)	1594
SinI	GGWCC	4 cut(s)	739, 1174, 1643, 2920
SmiMI	CAYNNNNRTG	3 cut(s)	1287, 1862, 3002
SmlI	CTYRAG	5 cut(s)	367, 2011, 2633, 3272, 3315
SmoI	CTYRAG	5 cut(s)	367, 2011, 2633, 3272, 3315
SpeI	ACTAGT	1 cut(s)	3149
SsiI	CCGC	2 cut(s)	30, 3225
SspI	AATATT	1 cut(s)	2945
SspMI	CTAG	5 cut(s)	983, 1059, 1482, 3038, 3150
SstI	GAGCTC	1 cut(s)	3316
StyD4I	CCNGG	2 cut(s)	1947, 2526
StyI	CCWWGG	4 cut(s)	735, 939, 1058, 1449
TaiI	ACGT	5 cut(s)	95, 1800, 2484, 3238, 3262
TaqI	TCGA	9 cut(s)	227, 236, 535, 712, 1554, 1594, 2381, 2423, 3305
TatI	WGTACW	2 cut(s)	1970, 2385
TauI	GCSGC	1 cut(s)	3227
Tru1I	TTAA	9 cut(s)	1046, 1313, 1718, 2042, 2228, 2883, 2891, 2987, 3033
Tru9I	TTAA	9 cut(s)	1046, 1313, 1718, 2042, 2228, 2883, 2891, 2987, 3033
TscAI	CASTG	9 cut(s)	367, 434, 1034, 1356, 1401, 1933, 2005, 2329, 2725
TseFI	GTSAC	6 cut(s)	177, 1241, 1466, 1801, 2200, 2639
TseI	GCWGC	9 cut(s)	587, 1220, 2629, 2804, 2807, 2855, 3179, 3227, 3230
Tsp45I	GTSAC	6 cut(s)	177, 1241, 1466, 1801, 2200, 2639
TspGWI	ACGGA	2 cut(s)	1611, 2921
TspRI	CASTG	9 cut(s)	367, 434, 1034, 1356, 1401, 1933, 2005, 2329, 2725
Tth111I	GACNNNGTC	1 cut(s)	2000
Van91I	CCANNNNNTGG	1 cut(s)	1223
VneI	GTGCAC	1 cut(s)	1855
VpaK11BI	GGWCC	4 cut(s)	739, 1174, 1643, 2920
XagI	CCTNNNNNAGG	1 cut(s)	2457
XapI	RAATTY	5 cut(s)	381, 1543, 1700, 1909, 2953
XceI	RCATGY	2 cut(s)	481, 1286
XmaJI	CCTAGG	1 cut(s)	1058
XmiI	GTMKAC	1 cut(s)	935
XmnI	GAANNNNTTC	3 cut(s)	93, 1107, 1545
XspI	CTAG	5 cut(s)	983, 1059, 1482, 3038, 3150
Zsp2I	ATGCAT	2 cut(s)	1163, 2355

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.