RLG00000024829

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002511
Physical Location & Seq
Reverse (-)
39473974 .. 39482435
8462 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024829

Sequence Viewer

Length: 3393 bp
ATGTTCGCCAAGCGACTCTTACAGAAGGCAGTCAACCACCACCACCATTCTCAGCATAGTTTGCAACAAGAAAGTATGACGTCAGCAGACTTGGACCCGCGAGTTGCAGTTCACTATGGTATTCCATCCACGGCATCCATTCTTGCTTTCGACCCCACTCAGCGGCTTCTGAAAGGACTCATGACCATACATATGGTCTGTGAACTCCAGATGAACTTTTCCAAGTTGTGGGAATTACTTAACAAAGTCTATGCCTTAACGTTACGTTTATATAAGTATCTGATACACCTTTTCCTTTCATTTCTACATAACCAGGGTTACTTGGTCAGCATCTTAAATGATAATAACATTGAGGTTTGGAATCTTGAGGGCAGATGCCTAGTTTGTTCTCTAGAGTGGGAATCAAATATAACTGCTTTTTCTGTAATCCATGGCTCGAACTTAATGTATGTGGGCGATGAATATGCTCTATTGGCTGTGGTGAAGTATGATGCTGACGAGGGAAAACTTTTGCAGTTGCCATATCATATATCTGCAGATTCATTAAGCGAAGCTGCTGAGTTTCCATTTCCTACCGACCAACCGATAGTTGGACTTCTTCCCCAACCTGGTTCATCTGGAAATAGAGTACTGATTGCGTATCAAAATGGGTTGCTTATTCTTTGGGACGTGTCCGAGGCTCAAATAGTTTTTATTGGAGGTGGTAAGGATCTCCAACTAAAGGATGGAGTTGTTAAGCCTACTGATGAAGTCAATACAGATTCTCCAGAAAATACAATAGAACATCAGCTGGGAGAGAAAGAGATAAGTGCTCTTTGTTGGGCATCATCCAATGGGTCCATTCTGGCTGTGGGATATGTAGATGGAGATATTCTGTTCTGGAACACGTCATCTGCTGCTTCCATTAAGGGTCAGCAAGCATTGTCATCATCTAATAATGTTGTTAAGTTACGATTATCATCATCAGAAAGAAGACTTCCTGTCATTGTCTTACAGTGGTCCACATTAAATAAATCCCATAATGATTCTGATGGACAACTATTTATCTATGGTGGCGATGAAATAGGATCAGACGAAGTTCTGACGGTTTTGACCCTTGATTGGTCTTCTGGGATGGGGAATTTAAGATGTGTTGGCCGTACGGACCTTACCCTTACTGGTTCGTTTGCAGACATGATTTTATTACCAAATTTTGCAGCAATTGCGGGTAATCACAGAGCTGATGCATTTGTGTTAACAAACCCTGGGCAATTGCACTTTTATGATGAAACCAGTCTCTCGGCCCTAATATCCCAGCAAGAAAAGAAACCATCTGTCTCTGCTTTGGAGTTTCCAGTGGTGATACCTACCACTAATCCGACCATGACTGCTGCCAGACTTATTAGGACAGCTACTGGGGAGAACTCGTTAAAGTCTCTATCAGAGTTATGCTCAGTTGTGAACCATGGCTCACTACCAACTTCATTAGATGGTACAAAATGGCCCTTGACTGGGGGTGTGCCCAGTCAATTGTCTCTTTCGAAGAGTAGTGGTATTGAGAGAGTATATCTAGCAGGATATTCAGATGGATCTGTTCGGATCTGGAATGCCACCTATCCGCTCTTATCTTTTATCTCTGTTTTAGAAGGCGAGGTGCAAGATATAAAAGTGGCTGGTTTAAGTTCTCCGGTATCGAGAATGGATTTCTGCAGCTTCACTTTAAACTTGGCTGTTGGCAGTGAATCTGGTTTGGTTCGAATTTATAACCTCAAAGGGCGTTCAGATGGGGTAAAATTCCTCTTTGTGACCGAAACCAAATGTGAAGCCCACAGTTTGTCTCAAGGAAAAGGACCTCAATGCAGAGCTGTTTTCAGTCTTACTAATTCCCGAGTTCAAGCACTTCAGTTTGTGAAGCATGGAGGTAAACTTGCAGTGGGATTTGAATGCGGTCATGTTGCAATGCTGGACACAAGTTCCTTATCTATATTTTTCTTCATAAAAGATGCATATTTCTCTAGCTCCCCAGTCATTTCAATGACTTGGAAAGAAATTACAAATCCTAAAGGCCTCTTGCAGAGTTCAAAGCTCTCGGAAACTAAATCACCAGTTCACCCTGCAGAGGAAGTAATGTTCATTCTAACCAAAGATGCAAATATACATTTAATTTGTGGAAACACGGGTAATATGATCATCCCTCGGCCATGGCACTTGAAAAAGGAGTCAATTGCAATTTCAATGTATGTTATAGATGGCAGAATTTCAGCCTCTAAAGTATCTGATGCAAATCCGCGGGAAGAGGCCTCAAAGGATACTTCAACCAAGAATGAGTCTATGGCAGGCAGTTCTCCAATTCCTATAGATTCACTTGAGGTGGACCAGGACTATAATTCAGAAAATGCATACTCTGAAGAAAGATTGTTGAATTCACTGATTTTGCTTTGTTGTGTGGATTCAGTGCAATTGTACTCCACAAAATCTGTGATTCAGGGAAACAATGAACCCATTCGAAAGGTGAAACATGCAAGACCTTGCATTTGGACGGCAACTCTGAAAAAAGTTGAAAAAGTTTGGGGATTGACTTTGCTGTTTCAGACTGGAGAGATTGAAATTAGATCTATTCCTGATTTAGAATTGGTGAAAGAAAGCTCCTTAATGTCAATTCTAAGGTGGAATTGTAAGGCAAACATGGATAAGACAATGAGTTCCGATGATGCACATATCACACTGGCAAATGGGTATGAAACGGTATTCATCTCTCTGTTAGCTGCTGAAAATGATTTCAGGATTCCAGAGTCTTTGCCTTGTCTCCATGATGCAGTTCTTGCAGCTGCTGCAGATGCTGCATTAAGTGCCTCTTTAAATCAGAAGAAGAAACAGAGCACTGCCCCTGCGATATTAGGCGGTATTGTCAAAGGCTTGAAAGGAGGAAAAATGGTTCAGAGTGGAGATTCTACTGCAACTCCGAAATCCAGGTTTGATCATTTAGAAGGCATGTTTTGCAAGTCCCAGCAGTGGGACTCGGTCCCAGCTTTGGATCATCAAGAAAATCTGGAGCTTAATATAGATGACATTGAAATTGATGAACCTCTACGCGTGACATCTACTTCAACATCCCATGATGATAATAATAAAAGGAGAGAAGGGGACTCAGAAAGGGATAGATTATTTCAAGGTGGGACTAGTGATACAAAGCCCAGAGTTAGGACTGCTGAAGAAGTTAAAGCTAAATATAGAAAAGCTGAGGATGCTTCTTCGGTGGCTTCCCAAGCAAGAAATAAGCTCATGGAGAGGGGGCAAAAACTTGAGAAACTCAGCAAGCGCACTGAGGATCTACAGAATGGAGCCGAAGATTTTGCATCAATGGCGAATGAGCTCGTCAAGACACTAGAAGCCCGAAAATGGTGGCATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000137 GO:0000139 GO:0000902 GO:0000904 GO:0002791 GO:0003674 GO:0005096 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005795 GO:0005802 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006892 GO:0006893 GO:0006996 GO:0007010 GO:0007275 GO:0007399 GO:0007409 GO:0008047 GO:0008150 GO:0009653 GO:0009987 GO:0010008 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0017016 GO:0017137 GO:0017157 GO:0019899 GO:0019900 GO:0019901 GO:0022008 GO:0022607 GO:0030029 GO:0030030 GO:0030036 GO:0030154 GO:0030182 GO:0030234 GO:0030695 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0031090 GO:0031175 GO:0031267 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0031985 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048193 GO:0048468 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050708 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051046 GO:0051049 GO:0051179 GO:0051223 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0060627 GO:0061564 GO:0065003 GO:0065007 GO:0065009 GO:0070201 GO:0071840 GO:0071944 GO:0090087 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0098876 GO:0099568 GO:0120036 GO:0120039 GO:1903530
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 Synaptobrevin

Protein Analysis

1131

Amino Acids

124.26

Weight (kDa)

5.78

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Synaptobrevin PF00957 1084 - 1124 2.6e-06 Synaptobrevin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1743
AatII GACGTC 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 228
AccBSI CCGCTC 1 cut(s) 1600
AccII CGCG 3 cut(s) 100, 2269, 3072
AciI CCGC 8 cut(s) 98, 163, 1205, 1598, 1926, 2267, 2269, 2880
AclI AACGTT 1 cut(s) 260
AclWI GGATC 6 cut(s) 717, 1075, 1576, 1586, 3021, 3315
AcoI YGGCCR 2 cut(s) 1135, 2177
AcsI RAATTY 6 cut(s) 1120, 1189, 1737, 1772, 2235, 2401
AcuI CTGAAG 3 cut(s) 1865, 2406, 3210
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 4 cut(s) 630, 1141, 1474, 2444
AflIII ACRYGT 3 cut(s) 669, 885, 3070
AhdI GACNNNNNGTC 1 cut(s) 980
AhlI ACTAGT 1 cut(s) 3158
AjiI CACGTC 2 cut(s) 670, 888
AjnI CCWGG 5 cut(s) 312, 607, 1243, 2355, 2948
AloI GAACNNNNNNTCC 4 cut(s) 2093, 2125, 2898, 2930
Alw21I GWGCWC 3 cut(s) 814, 2861, 3354
Alw26I GTCTC 6 cut(s) 1280, 1321, 1419, 1518, 1821, 2789
AlwI GGATC 6 cut(s) 717, 1075, 1576, 1586, 3021, 3315
AlwNI CAGNNNCTG 3 cut(s) 1394, 2810, 2819
Ama87I CYCGRG 1 cut(s) 1866
AoxI GGCC 6 cut(s) 1135, 1281, 1481, 2044, 2177, 2277
ApoI RAATTY 6 cut(s) 1120, 1189, 1737, 1772, 2235, 2401
ArsI GACNNNNNNTTYG 2 cut(s) 2496, 2528
Asp700I GAANNNNTTC 1 cut(s) 2481
AspLEI GCGC 1 cut(s) 3300
AspS9I GGNCC 9 cut(s) 94, 837, 999, 1144, 1282, 1482, 1829, 2353, 3001
AsuHPI GGTGA 6 cut(s) 493, 1351, 2073, 2081, 2503, 2626
AsuII TTCGAA 3 cut(s) 1520, 1735, 2485
AvaI CYCGRG 1 cut(s) 1866
AvaII GGWCC 7 cut(s) 94, 837, 999, 1144, 1829, 2353, 3001
BaeGI GKGCMC 1 cut(s) 1503
BanII GRGCYC 1 cut(s) 3354
BbsI GAAGAC 2 cut(s) 979, 1098
Bbv12I GWGCWC 3 cut(s) 814, 2861, 3354
BbvCI CCTCAGC 1 cut(s) 3219
BceAI ACGGC 3 cut(s) 147, 1122, 2535
BcgI CGANNNNNNTGC 4 cut(s) 446, 480, 3314, 3348
BciT130I CCWGG 5 cut(s) 314, 609, 1245, 2357, 2950
BciVI GTATCC 1 cut(s) 2281
BclI TGATCA 2 cut(s) 2166, 2956
BcoDI GTCTC 6 cut(s) 1280, 1321, 1419, 1518, 1821, 2789
BcuI ACTAGT 1 cut(s) 3158
BfaI CTAG 6 cut(s) 380, 392, 1550, 1995, 3159, 3365
BfmI CTRYAG 6 cut(s) 534, 1687, 2094, 2334, 2811, 3311
BfuI GTATCC 1 cut(s) 2281
BglII AGATCT 1 cut(s) 2591
BmcAI AGTACT 1 cut(s) 630
Bme1390I CCNGG 5 cut(s) 314, 609, 1245, 2357, 2950
Bme18I GGWCC 7 cut(s) 94, 837, 999, 1144, 1829, 2353, 3001
BmeRI GACNNNNNGTC 1 cut(s) 980
BmeT110I CYCGRG 1 cut(s) 1866
BmgBI CACGTC 2 cut(s) 670, 888
BmgT120I GGNCC 9 cut(s) 94, 837, 999, 1144, 1282, 1482, 1829, 2353, 3001
BmiI GGNNCC 4 cut(s) 96, 838, 3003, 3322
BmrFI CCNGG 5 cut(s) 314, 609, 1245, 2357, 2950
BmrI ACTGGG 4 cut(s) 1404, 1497, 1500, 1997
BmuI ACTGGG 4 cut(s) 1404, 1497, 1500, 1997
BpiI GAAGAC 2 cut(s) 979, 1098
BplI GAGNNNNNCTC 2 cut(s) 1415, 1447
BpmI CTGGAG 4 cut(s) 191, 750, 2595, 3050
Bpu10I CCTNAGC 1 cut(s) 3219
Bpu14I TTCGAA 3 cut(s) 1520, 1735, 2485
BpuEI CTTGAG 4 cut(s) 386, 1803, 2366, 3302
BsaBI GATNNNNATC 2 cut(s) 958, 2262
BsaHI GRCGYC 1 cut(s) 80
BsaWI WCCGGW 1 cut(s) 1666
BsaXI ACNNNNNCTCC 6 cut(s) 748, 778, 1391, 1421, 2923, 2953
Bse3DI GCAATG 1 cut(s) 1944
Bse8I GATNNNNATC 2 cut(s) 958, 2262
BseBI CCWGG 5 cut(s) 314, 609, 1245, 2357, 2950
BseGI GGATG 8 cut(s) 125, 134, 730, 827, 1119, 2169, 3089, 3229
BseJI GATNNNNATC 2 cut(s) 958, 2262
BseMI GCAATG 1 cut(s) 1944
BseMII CTCAG 8 cut(s) 65, 173, 549, 1446, 3141, 3210, 3294, 3304
BseSI GKGCMC 1 cut(s) 1503
BseYI CCCAGC 4 cut(s) 790, 1293, 2985, 3004
Bsh1236I CGCG 3 cut(s) 100, 2269, 3072
BshFI GGCC 6 cut(s) 1137, 1283, 1483, 2046, 2179, 2279
BsiHKAI GWGCWC 3 cut(s) 814, 2861, 3354
BsiHKCI CYCGRG 1 cut(s) 1866
BsiSI CCGG 1 cut(s) 1667
BsiWI CGTACG 1 cut(s) 1139
BslFI GGGAC 6 cut(s) 680, 2968, 2987, 3008, 3137, 3169
BsmAI GTCTC 6 cut(s) 1280, 1321, 1419, 1518, 1821, 2789
BsmFI GGGAC 6 cut(s) 680, 2968, 2987, 3008, 3137, 3169
BsmI GAATGC 2 cut(s) 1591, 1928
BsnI GGCC 6 cut(s) 1137, 1283, 1483, 2046, 2179, 2279
BsoBI CYCGRG 1 cut(s) 1866
Bsp119I TTCGAA 3 cut(s) 1520, 1735, 2485
Bsp1286I GDGCHC 4 cut(s) 814, 1503, 2861, 3354
Bsp143I GATC 9 cut(s) 709, 1067, 1568, 1578, 2166, 2591, 2956, 3013, 3307
Bsp19I CCATGG 3 cut(s) 430, 1444, 2180
BspACI CCGC 8 cut(s) 98, 163, 1205, 1598, 1926, 2267, 2269, 2880
BspANI GGCC 6 cut(s) 1137, 1283, 1483, 2046, 2179, 2279
BspCNI CTCAG 8 cut(s) 64, 172, 550, 1445, 3140, 3211, 3295, 3303
BspFNI CGCG 3 cut(s) 100, 2269, 3072
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 4 cut(s) 96, 838, 3003, 3322
BspMAI CTGCAG 4 cut(s) 538, 1691, 2098, 2815
BspPI GGATC 6 cut(s) 717, 1075, 1576, 1586, 3021, 3315
BspT104I TTCGAA 3 cut(s) 1520, 1735, 2485
BsrBI CCGCTC 1 cut(s) 1600
BsrDI GCAATG 1 cut(s) 1944
BssMI GATC 9 cut(s) 709, 1067, 1568, 1578, 2166, 2591, 2956, 3013, 3307
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 3 cut(s) 430, 1444, 2180
Bst2UI CCWGG 5 cut(s) 314, 609, 1245, 2357, 2950
Bst4CI ACNGT 4 cut(s) 996, 1087, 1811, 2725
Bst6I CTCTTC 2 cut(s) 1517, 2268
BstACI GRCGYC 1 cut(s) 80
BstAPI GCANNNNNTGC 7 cut(s) 61, 1202, 2801, 2810, 2819, 2828, 2976
BstBI TTCGAA 3 cut(s) 1520, 1735, 2485
BstC8I GCNNGC 3 cut(s) 918, 2317, 3296
BstDEI CTNAG 9 cut(s) 51, 159, 558, 1432, 2642, 3127, 3219, 3290, 3303
BstDSI CCRYGG 5 cut(s) 129, 430, 1444, 2180, 2267
BstF5I GGATG 8 cut(s) 125, 134, 730, 827, 1119, 2169, 3089, 3229
BstFNI CGCG 3 cut(s) 100, 2269, 3072
BstHHI GCGC 1 cut(s) 3300
BstKTI GATC 9 cut(s) 712, 1070, 1571, 1581, 2169, 2594, 2959, 3016, 3310
BstMAI GTCTC 6 cut(s) 1280, 1321, 1419, 1518, 1821, 2789
BstMBI GATC 9 cut(s) 709, 1067, 1568, 1578, 2166, 2591, 2956, 3013, 3307
BstNI CCWGG 5 cut(s) 314, 609, 1245, 2357, 2950
BstNSI RCATGY 2 cut(s) 2501, 2974
BstSCI CCNGG 5 cut(s) 312, 607, 1243, 2355, 2948
BstSFI CTRYAG 6 cut(s) 534, 1687, 2094, 2334, 2811, 3311
BstSLI GKGCMC 1 cut(s) 1503
BstUI CGCG 3 cut(s) 100, 2269, 3072
BstV2I GAAGAC 2 cut(s) 979, 1098
BstX2I RGATCY 5 cut(s) 709, 1568, 1578, 2591, 3307
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 5 cut(s) 709, 1568, 1578, 2591, 3307
BsuI GTATCC 1 cut(s) 2281
BsuRI GGCC 6 cut(s) 1137, 1283, 1483, 2046, 2179, 2279
BtgI CCRYGG 5 cut(s) 129, 430, 1444, 2180, 2267
BtgZI GCGATG 2 cut(s) 471, 1071
BtrI CACGTC 2 cut(s) 670, 888
BtsCI GGATG 8 cut(s) 125, 134, 730, 827, 1119, 2169, 3089, 3229
BtsI GCAGTG 4 cut(s) 1723, 1917, 2859, 2996
Cac8I GCNNGC 3 cut(s) 918, 2317, 3296
CaiI CAGNNNCTG 3 cut(s) 1394, 2810, 2819
CciI TCATGA 1 cut(s) 180
CfoI GCGC 1 cut(s) 3300
Cfr13I GGNCC 9 cut(s) 94, 837, 999, 1144, 1282, 1482, 1829, 2353, 3001
Cfr42I CCGCGG 1 cut(s) 2270
CsiI ACCWGGT 1 cut(s) 607
Csp6I GTAC 4 cut(s) 629, 1140, 1473, 2443
CviQI GTAC 4 cut(s) 629, 1140, 1473, 2443
DdeI CTNAG 9 cut(s) 51, 159, 558, 1432, 2642, 3127, 3219, 3290, 3303
DpnI GATC 9 cut(s) 711, 1069, 1570, 1580, 2168, 2593, 2958, 3015, 3309
DpnII GATC 9 cut(s) 709, 1067, 1568, 1578, 2166, 2591, 2956, 3013, 3307
DraI TTTAAA 2 cut(s) 1701, 2838
DriI GACNNNNNGTC 1 cut(s) 980
EaeI YGGCCR 2 cut(s) 1135, 2177
Eam1104I CTCTTC 2 cut(s) 1517, 2268
Eam1105I GACNNNNNGTC 1 cut(s) 980
EarI CTCTTC 2 cut(s) 1517, 2268
Ecl136II GAGCTC 1 cut(s) 3352
Eco130I CCWWGG 3 cut(s) 430, 1444, 2180
Eco147I AGGCCT 2 cut(s) 2046, 2279
Eco24I GRGCYC 1 cut(s) 3354
Eco47I GGWCC 7 cut(s) 94, 837, 999, 1144, 1829, 2353, 3001
Eco53kI GAGCTC 1 cut(s) 3352
Eco57I CTGAAG 3 cut(s) 1865, 2406, 3210
Eco88I CYCGRG 1 cut(s) 1866
EcoICRI GAGCTC 1 cut(s) 3352
EcoO109I RGGNCCY 1 cut(s) 1829
EcoRI GAATTC 1 cut(s) 2401
EcoRII CCWGG 5 cut(s) 312, 607, 1243, 2355, 2948
EcoT14I CCWWGG 3 cut(s) 430, 1444, 2180
EcoT22I ATGCAT 3 cut(s) 1228, 1987, 2380
EcoT38I GRGCYC 1 cut(s) 3354
ErhI CCWWGG 3 cut(s) 430, 1444, 2180
FalI AAGNNNNNCTT 3 cut(s) 34, 2818, 2850
FaqI GGGAC 6 cut(s) 680, 2968, 2987, 3008, 3137, 3169
FauI CCCGC 3 cut(s) 105, 1198, 2262
FauNDI CATATG 1 cut(s) 192
FbaI TGATCA 2 cut(s) 2166, 2956
FokI GGATG 8 cut(s) 112, 121, 737, 814, 1126, 2156, 3076, 3236
FriOI GRGCYC 1 cut(s) 3354
FspBI CTAG 6 cut(s) 380, 392, 1550, 1995, 3159, 3365
GlaI GCGC 1 cut(s) 3299
GsaI CCCAGC 4 cut(s) 794, 1297, 2989, 3008
GsuI CTGGAG 4 cut(s) 191, 750, 2595, 3050
HaeIII GGCC 6 cut(s) 1137, 1283, 1483, 2046, 2179, 2279
HapII CCGG 1 cut(s) 1667
HhaI GCGC 1 cut(s) 3300
Hin1I GRCGYC 1 cut(s) 80
Hin6I GCGC 1 cut(s) 3298
HinP1I GCGC 1 cut(s) 3298
HincII GTYRAC 2 cut(s) 34, 1236
HindII GTYRAC 2 cut(s) 34, 1236
HpaI GTTAAC 1 cut(s) 1236
HpaII CCGG 1 cut(s) 1667
HphI GGTGA 6 cut(s) 493, 1351, 2073, 2081, 2503, 2626
Hpy166II GTNNAC 9 cut(s) 34, 112, 203, 1002, 1236, 1441, 1904, 2089, 2353
Hpy8I GTNNAC 9 cut(s) 34, 112, 203, 1002, 1236, 1441, 1904, 2089, 2353
HpyAV CCTTC 4 cut(s) 19, 1619, 2960, 3113
HpyCH4III ACNGT 4 cut(s) 996, 1087, 1811, 2725
HpyCH4IV ACGT 5 cut(s) 80, 260, 265, 669, 887
HpyF3I CTNAG 9 cut(s) 51, 159, 558, 1432, 2642, 3127, 3219, 3290, 3303
HpySE526I ACGT 5 cut(s) 80, 260, 265, 669, 887
Hsp92I GRCGYC 1 cut(s) 80
HspAI GCGC 1 cut(s) 3298
Ksp22I TGATCA 2 cut(s) 2166, 2956
KspAI GTTAAC 1 cut(s) 1236
KspI CCGCGG 1 cut(s) 2270
Kzo9I GATC 9 cut(s) 709, 1067, 1568, 1578, 2166, 2591, 2956, 3013, 3307
LmnI GCTCC 4 cut(s) 2003, 2630, 3031, 3320
MabI ACCWGGT 1 cut(s) 607
MaeI CTAG 6 cut(s) 380, 392, 1550, 1995, 3159, 3365
MaeII ACGT 5 cut(s) 80, 260, 265, 669, 887
MaeIII GTNAC 5 cut(s) 261, 317, 948, 1783, 3073
MalI GATC 9 cut(s) 711, 1069, 1570, 1580, 2168, 2593, 2958, 3015, 3309
MbiI CCGCTC 1 cut(s) 1600
MboI GATC 9 cut(s) 709, 1067, 1568, 1578, 2166, 2591, 2956, 3013, 3307
MfeI CAATTG 5 cut(s) 1200, 1250, 1508, 2202, 2438
MflI RGATCY 5 cut(s) 709, 1568, 1578, 2591, 3307
MhlI GDGCHC 4 cut(s) 814, 1503, 2861, 3354
MluI ACGCGT 1 cut(s) 3070
MlyI GAGTC 7 cut(s) 9, 171, 2207, 2315, 2780, 2990, 3119
MmeI TCCRAC 3 cut(s) 571, 739, 1382
Mph1103I ATGCAT 3 cut(s) 1228, 1987, 2380
MroXI GAANNNNTTC 1 cut(s) 2481
MslI CAYNNNNRTG 3 cut(s) 191, 1260, 2012
MspA1I CMGCKG 4 cut(s) 163, 790, 2269, 2807
MspI CCGG 1 cut(s) 1667
MspR9I CCNGG 5 cut(s) 314, 609, 1245, 2357, 2950
MunI CAATTG 5 cut(s) 1200, 1250, 1508, 2202, 2438
Mva1269I GAATGC 2 cut(s) 1591, 1928
MvaI CCWGG 5 cut(s) 314, 609, 1245, 2357, 2950
MvnI CGCG 3 cut(s) 100, 2269, 3072
NcoI CCATGG 3 cut(s) 430, 1444, 2180
NdeI CATATG 1 cut(s) 192
NdeII GATC 9 cut(s) 709, 1067, 1568, 1578, 2166, 2591, 2956, 3013, 3307
NlaIV GGNNCC 4 cut(s) 96, 838, 3003, 3322
NmeAIII GCCGAG 2 cut(s) 1259, 2155
NmuCI GTSAC 2 cut(s) 1783, 3073
NsiI ATGCAT 3 cut(s) 1228, 1987, 2380
NspI RCATGY 2 cut(s) 2501, 2974
NspV TTCGAA 3 cut(s) 1520, 1735, 2485
PagI TCATGA 1 cut(s) 180
PasI CCCWGGG 1 cut(s) 1244
PceI AGGCCT 2 cut(s) 2046, 2279
PctI GAATGC 2 cut(s) 1591, 1928
PdmI GAANNNNTTC 1 cut(s) 2481
Pfl23II CGTACG 1 cut(s) 1139
PflFI GACNNNGTC 1 cut(s) 2999
PflMI CCANNNNNTGG 1 cut(s) 228
PleI GAGTC 7 cut(s) 9, 171, 2206, 2314, 2779, 2990, 3119
PpsI GAGTC 7 cut(s) 9, 171, 2206, 2314, 2779, 2990, 3119
PpuMI RGGWCCY 1 cut(s) 1829
PsiI TTATAA 1 cut(s) 1743
Psp124BI GAGCTC 1 cut(s) 3354
Psp1406I AACGTT 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 1829
Psp6I CCWGG 5 cut(s) 312, 607, 1243, 2355, 2948
PspFI CCCAGC 4 cut(s) 790, 1293, 2985, 3004
PspGI CCWGG 5 cut(s) 312, 607, 1243, 2355, 2948
PspLI CGTACG 1 cut(s) 1139
PspN4I GGNNCC 4 cut(s) 96, 838, 3003, 3322
PspPI GGNCC 9 cut(s) 94, 837, 999, 1144, 1282, 1482, 1829, 2353, 3001
PspPPI RGGWCCY 1 cut(s) 1829
PstI CTGCAG 4 cut(s) 538, 1691, 2098, 2815
PstNI CAGNNNCTG 3 cut(s) 1394, 2810, 2819
PsuI RGATCY 5 cut(s) 709, 1568, 1578, 2591, 3307
PsyI GACNNNGTC 1 cut(s) 2999
PvuII CAGCTG 2 cut(s) 790, 2807
RsaI GTAC 4 cut(s) 630, 1141, 1474, 2444
RsaNI GTAC 4 cut(s) 629, 1140, 1473, 2443
RseI CAYNNNNRTG 3 cut(s) 191, 1260, 2012
SacI GAGCTC 1 cut(s) 3354
SacII CCGCGG 1 cut(s) 2270
Sau3AI GATC 9 cut(s) 709, 1067, 1568, 1578, 2166, 2591, 2956, 3013, 3307
Sau96I GGNCC 9 cut(s) 94, 837, 999, 1144, 1282, 1482, 1829, 2353, 3001
ScaI AGTACT 1 cut(s) 630
SchI GAGTC 7 cut(s) 9, 171, 2207, 2315, 2780, 2990, 3119
ScrFI CCNGG 5 cut(s) 314, 609, 1245, 2357, 2950
SduI GDGCHC 4 cut(s) 814, 1503, 2861, 3354
SexAI ACCWGGT 1 cut(s) 607
SfcI CTRYAG 6 cut(s) 534, 1687, 2094, 2334, 2811, 3311
Sfr303I CCGCGG 1 cut(s) 2270
SfuI TTCGAA 3 cut(s) 1520, 1735, 2485
SgrBI CCGCGG 1 cut(s) 2270
SinI GGWCC 7 cut(s) 94, 837, 999, 1144, 1829, 2353, 3001
SmiMI CAYNNNNRTG 3 cut(s) 191, 1260, 2012
SmlI CTYRAG 4 cut(s) 365, 1818, 2345, 3281
SmoI CTYRAG 4 cut(s) 365, 1818, 2345, 3281
SpeI ACTAGT 1 cut(s) 3158
SseBI AGGCCT 2 cut(s) 2046, 2279
SsiI CCGC 8 cut(s) 98, 163, 1205, 1598, 1926, 2267, 2269, 2880
SspMI CTAG 6 cut(s) 380, 392, 1550, 1995, 3159, 3365
SstI GAGCTC 1 cut(s) 3354
StuI AGGCCT 2 cut(s) 2046, 2279
StyD4I CCNGG 5 cut(s) 312, 607, 1243, 2355, 2948
StyI CCWWGG 3 cut(s) 430, 1444, 2180
TaaI ACNGT 4 cut(s) 996, 1087, 1811, 2725
TaiI ACGT 5 cut(s) 83, 263, 268, 672, 890
TaqI TCGA 6 cut(s) 150, 437, 1520, 1673, 1735, 2485
TaqII GACCGA 2 cut(s) 1802, 2989
TatI WGTACW 2 cut(s) 628, 2442
TauI GCSGC 1 cut(s) 166
TseFI GTSAC 2 cut(s) 1783, 3073
Tsp45I GTSAC 2 cut(s) 1783, 3073
TspGWI ACGGA 1 cut(s) 1157
Tth111I GACNNNGTC 1 cut(s) 2999
Van91I CCANNNNNTGG 1 cut(s) 228
VpaK11BI GGWCC 7 cut(s) 94, 837, 999, 1144, 1829, 2353, 3001
XapI RAATTY 6 cut(s) 1120, 1189, 1737, 1772, 2235, 2401
XbaI TCTAGA 1 cut(s) 391
XceI RCATGY 2 cut(s) 2501, 2974
XcmI CCANNNNNNNNNTGG 3 cut(s) 587, 722, 847
XmnI GAANNNNTTC 1 cut(s) 2481
XspI CTAG 6 cut(s) 380, 392, 1550, 1995, 3159, 3365
ZraI GACGTC 1 cut(s) 81
ZrmI AGTACT 1 cut(s) 630
Zsp2I ATGCAT 3 cut(s) 1228, 1987, 2380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.