pycom04g14030

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002511
Physical Location & Seq
Reverse (-)
16933950 .. 16939875
5926 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g14030.9

Sequence Viewer

Length: 2631 bp
ATGATAATGATATTCAGAACTCCATATAACTTCTGCATCGTATGCATCTTCATTAAGTTATTGGCGATTACAAACTTCAGTGTTTGGAATCTCGAGAGCAGGTCCCTAGTTTATTGTCTAGAGTGGGAAACAAATATAACCGCATTTTCTGTAATCCATGGCTCCAATTTAATGTACACTACTGATCGAAAAAATTGTTACAACTATTTTGAGTCAGCTACTAATTGGTATGTTGGTGATGAGTATGCTCTAGTGGCTGTGGTGAAGTATGATGCAGAGGAGGGAAAACTTTTGCGATTGCCATATCATATTTCTGCAAATTCTTTATATGGTTTTTCTATTGGCAGAGTATTGATTGCATATCAAAATGGGTTGGTTATTCTTTGGGATGTATCTGAAGCTAAAATAGCTTTTATTGGAGGTGGTAAGGATCTCCAACTAAGGGATGGAGTTGTTAAATCTACACATGAAGTCAATGTTGATTCTCCAGAAGATACATTGGAACAGCAATTGGGAGACAAAGAGATAAGTGCTCTTTGTTGGGCATCTGCGAATGGCTCCATTCTGGCTGTGGGATACATAGATGGAGACATTTTATTCTGGAACATGTCATCTCCTGCTTCCGTCAAGGGTCAAAAAGCTTTATCGCCATCTAATAATGTTGTGAAGTTACAATTATCATCTGCTGAAAGGAGACTTCCTGTCATAGTTTTACAGTGGTCCACAGATTATAAATCCCATAATGGTTGTGATGGCCAACTATTTATCTATGGTGGCGATGAAATCGGATCAGAAGAAGTTTTGACGGTTCTAACTCTTGAATGGTCACCTGGAATGGGGAATTTAAGATGTGTTGGTCGTACGGACCTCACCCTTACTGGCTCTTTTGCAGACTTGATTTTGTTACCAAGTTCTGGGACAACCGGGGATAATCATAAAGCTGATGTTTTTGTTTTAACCAACCCTGGGCAACTGCATCTTTATGATGATGCTTGCCTCTCTGCCTTAACGTCCCAGAAAGAAAGGAGTCCATCTGTCTCTGCTTTGGAGTTTCCGGTGGTGATACCTACAACTGATCCAAGCATGACTGTTGCCAAACTTGTTAGGATATCCTCAGTTGTCAACCTTGGCTCAGCACAAACCCCATCAGCTGGTACAAAATGGCCCTTGACTGGGGGTGTTCCCAGTCAACTGTCTATTTCTGAGAATAATGGTATTGAGAGACTGTACCTAACAGGATATTCAGATGGATCTGTTCGGATATGGAATGCAACATATCCACTCTTCTCTTTTATCTGCCTCGTAGAAAGCAAGGTGCAAGGTATAAAAGTTGCTGGTTCAAGTGCTCCCGTATCACAAATGGATTTCTGCGCCTTCACTTTAAATCTGGCTGTTGGCAGTGAATGTGGTCTGGTTCGAATTTATAATCTGAAAGGCTGTTCCGATGGGGTAAAATTCATATATGTCACAGAAACCAAATGTGAAGTTCACGATTCGCCTCAAGCAAAAGGATTGCAATGCAGAGCTGTATTTAGTCTTACAAATTCCTCAGTTCAAGCACTTCAGTTTGTAAAGCATGGTGCTAAACTTGCTGTGGGATTTAAGTGTGGTCATGTTGCGGTGCTGGACACAAGTTCCTTTTCTGTATTGTTCTTTATAAAAGATGCATCTTTCTCCAGCTCTCCAGTCATTTCAATGACTTGGAAAGAACTTACAAATTCTCAACACCTCCTGAAGAGCCCAAAGGTTTCCGAAACAAAATCAACAGCTAACCCAGCAGAGGAGGTAATGTTTGTCTTAACCAAGGATGCAAATATACATGTAATTGATGGAAATGCTGGTAACCTGATCATCCCTCAGCCATGGCACTTGAAAAAGGAGTCAATTGCACTTTCAATGTATGTTATAGATGGCAGAGTATCTACGTCTGAAAATGTGTCTGATGATAATCCACCCCAAGAGGCCTCCAAGGATTCTTCAACAAAGAATGAGCCTATGCCAAGTGATACTCCAATTGTTACAAATTCACATGAAACTGAGCATGAATCCAGCTCAGAAAGTACATACTCTGAAGAGAGATTGTTGAATTCATTCATTTTGCTTTGTTGTGTGGATTCACTGCGGTTATACTCAACAAAATCTGTGATTCAGGGAAACAATAAACCCATCCGAAAAGTGAAACATGCAAGACCTTGCATTTGGACAGCAACTTTTAAGAGAGTTGAAAAGGTTTCGGGATTGGTTTTGCTGTTTCAGACTGGAGAAATTGAGATCAGATCCATTCCTGATTTAGAATTGGTGAAAGAAAGCTCCTTGATGTCAATTCTAAGGTGGAATTGTAAGGCAAACATGGATAAGACAATGAGTGCCGATGATGCACATATTACACTGGTAAATGGGTATGAATCGGCATTCATCTCTATATTAGCTGTTGAGAATGATTTCAGGATTCCAGAATCTTTGCCCTGTCTCCATGATAAAGTTGTTGCAGCTGCTGCAGAAGCTGCACTCAGTGTCTCTTTGAATCAGAAGAAAAAACAGGGTACTGTACCCGGAATATTAGGTGGTATTGTTAAAGGCTTTAAAGGGGGGAAAATGGTCCAGAGTGGAGATCGATACTGCAACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000137 GO:0000139 GO:0000902 GO:0000904 GO:0002791 GO:0003674 GO:0005096 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005795 GO:0005802 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006892 GO:0006893 GO:0006996 GO:0007010 GO:0007275 GO:0007399 GO:0007409 GO:0008047 GO:0008150 GO:0009653 GO:0009987 GO:0010008 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0017016 GO:0017137 GO:0017157 GO:0019899 GO:0019900 GO:0019901 GO:0022008 GO:0022607 GO:0030029 GO:0030030 GO:0030036 GO:0030154 GO:0030182 GO:0030234 GO:0030695 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0031090 GO:0031175 GO:0031267 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0031985 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048193 GO:0048468 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050708 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051046 GO:0051049 GO:0051179 GO:0051223 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0060627 GO:0061564 GO:0065003 GO:0065007 GO:0065009 GO:0070201 GO:0071840 GO:0071944 GO:0090087 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0098876 GO:0099568 GO:0120036 GO:0120039 GO:1903530
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 Synaptobrevin

Protein Analysis

877

Amino Acids

95.67

Weight (kDa)

5.8

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 732, 1425, 1658
Acc36I ACCTGC 1 cut(s) 90
AccB7I CCANNNNNTGG 2 cut(s) 914, 1151
AciI CCGC 3 cut(s) 141, 1619, 2122
AclWI GGATC 5 cut(s) 438, 796, 1070, 1258, 2271
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 8 cut(s) 319, 841, 1419, 1454, 1543, 1717, 2023, 2086
AcuI CTGAAG 5 cut(s) 61, 417, 1547, 1754, 2091
AdeI CACNNNGTG 1 cut(s) 2513
AfaI GTAC 7 cut(s) 176, 862, 1156, 1229, 2062, 2545, 2550
AfiI CCNNNNNNNGG 8 cut(s) 442, 836, 914, 966, 1151, 1172, 1173, 1780
AflIII ACRYGT 2 cut(s) 606, 1819
AhdI GACNNNNNGTC 1 cut(s) 701
AjnI CCWGG 2 cut(s) 829, 964
Alw21I GWGCWC 2 cut(s) 535, 1348
Alw26I GTCTC 7 cut(s) 510, 582, 688, 1042, 1216, 2474, 2521
AlwI GGATC 5 cut(s) 438, 796, 1070, 1258, 2271
AlwNI CAGNNNCTG 2 cut(s) 2495, 2504
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 3 cut(s) 754, 1163, 1962
ApeKI GCWGC 4 cut(s) 2489, 2492, 2495, 2504
ApoI RAATTY 8 cut(s) 319, 841, 1419, 1454, 1543, 1717, 2023, 2086
ArsI GACNNNNNNTTYG 2 cut(s) 2181, 2213
Asp700I GAANNNNTTC 2 cut(s) 2090, 2441
AspLEI GCGC 1 cut(s) 1373
AspS9I GGNCC 5 cut(s) 102, 720, 865, 1164, 2599
AsuC2I CCSGG 2 cut(s) 925, 2553
AsuHPI GGTGA 6 cut(s) 248, 274, 819, 862, 1072, 2311
AsuII TTCGAA 1 cut(s) 1417
AvaI CYCGRG 1 cut(s) 92
AvaII GGWCC 4 cut(s) 102, 720, 865, 2599
BalI TGGCCA 1 cut(s) 756
BanII GRGCYC 1 cut(s) 1742
Bbv12I GWGCWC 2 cut(s) 535, 1348
BbvCI CCTCAGC 1 cut(s) 1857
BbvI GCAGC 4 cut(s) 2479, 2482, 2491, 2501
BciT130I CCWGG 2 cut(s) 831, 966
BciVI GTATCC 1 cut(s) 569
BclI TGATCA 1 cut(s) 1848
BcnI CCSGG 2 cut(s) 925, 2553
BcoDI GTCTC 7 cut(s) 510, 582, 688, 1042, 1216, 2474, 2521
BfaI CTAG 3 cut(s) 107, 119, 251
BfmI CTRYAG 1 cut(s) 2496
BfuAI ACCTGC 1 cut(s) 90
BfuI GTATCC 1 cut(s) 569
BisI GCNGC 4 cut(s) 2490, 2493, 2496, 2505
BlpI GCTNAGC 1 cut(s) 1132
BlsI GCNGC 4 cut(s) 2491, 2494, 2497, 2506
Bme1390I CCNGG 4 cut(s) 831, 925, 966, 2553
Bme18I GGWCC 4 cut(s) 102, 720, 865, 2599
BmeRI GACNNNNNGTC 1 cut(s) 701
BmeT110I CYCGRG 1 cut(s) 92
BmgT120I GGNCC 5 cut(s) 102, 720, 865, 1164, 2599
BmiI GGNNCC 3 cut(s) 104, 163, 559
BmrFI CCNGG 4 cut(s) 831, 925, 966, 2553
BmrI ACTGGG 2 cut(s) 1179, 1182
BmuI ACTGGG 2 cut(s) 1179, 1182
BpmI CTGGAG 4 cut(s) 471, 1660, 1668, 2280
Bpu10I CCTNAGC 1 cut(s) 1857
Bpu1102I GCTNAGC 1 cut(s) 1132
Bpu14I TTCGAA 1 cut(s) 1417
BpuEI CTTGAG 1 cut(s) 1485
BpuMI CCSGG 2 cut(s) 925, 2553
Bsa29I ATCGAT 1 cut(s) 2614
BsaBI GATNNNNATC 1 cut(s) 1947
BsaJI CCNNGG 8 cut(s) 157, 924, 964, 965, 1126, 1803, 1862, 1968
BsaWI WCCGGW 1 cut(s) 1054
BsaXI ACNNNNNCTCC 3 cut(s) 1775, 1805, 2609
Bsc4I CCNNNNNNNGG 8 cut(s) 442, 836, 914, 966, 1151, 1172, 1173, 1780
Bse1I ACTGG 6 cut(s) 883, 1177, 1185, 1685, 2263, 2394
Bse3DI GCAATG 1 cut(s) 1523
Bse8I GATNNNNATC 1 cut(s) 1947
BseBI CCWGG 2 cut(s) 831, 966
BseCI ATCGAT 1 cut(s) 2614
BseDI CCNNGG 8 cut(s) 157, 924, 964, 965, 1126, 1803, 1862, 1968
BseGI GGATG 5 cut(s) 394, 451, 1813, 1851, 2166
BseJI GATNNNNATC 1 cut(s) 1947
BseLI CCNNNNNNNGG 8 cut(s) 442, 836, 914, 966, 1151, 1172, 1173, 1780
BseMI GCAATG 1 cut(s) 1523
BseMII CTCAG 8 cut(s) 1128, 1146, 1194, 1563, 1871, 2028, 2067, 2524
BseNI ACTGG 6 cut(s) 883, 1177, 1185, 1685, 2263, 2394
BseRI GAGGAG 2 cut(s) 293, 1796
BseXI GCAGC 4 cut(s) 2479, 2482, 2491, 2501
BseYI CCCAGC 1 cut(s) 1774
BsgI GTGCAG 1 cut(s) 2490
BshFI GGCC 3 cut(s) 756, 1165, 1964
BshVI ATCGAT 1 cut(s) 2614
BsiHKAI GWGCWC 2 cut(s) 535, 1348
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 3 cut(s) 924, 1055, 2553
BsiWI CGTACG 1 cut(s) 860
BslFI GGGAC 3 cut(s) 88, 931, 997
BslI CCNNNNNNNGG 8 cut(s) 442, 836, 914, 966, 1151, 1172, 1173, 1780
BsmAI GTCTC 7 cut(s) 510, 582, 688, 1042, 1216, 2474, 2521
BsmFI GGGAC 3 cut(s) 88, 931, 997
BsmI GAATGC 2 cut(s) 1273, 2411
BsnI GGCC 3 cut(s) 756, 1165, 1964
BsoBI CYCGRG 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 1417
Bsp1286I GDGCHC 3 cut(s) 535, 1348, 1742
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 9 cut(s) 184, 430, 788, 1075, 1250, 1848, 2271, 2276, 2611
Bsp1720I GCTNAGC 1 cut(s) 1132
Bsp19I CCATGG 2 cut(s) 157, 1862
BspACI CCGC 3 cut(s) 141, 1619, 2122
BspANI GGCC 3 cut(s) 756, 1165, 1964
BspCNI CTCAG 8 cut(s) 1127, 1145, 1195, 1562, 1870, 2029, 2066, 2523
BspDI ATCGAT 1 cut(s) 2614
BspLI GGNNCC 3 cut(s) 104, 163, 559
BspMAI CTGCAG 1 cut(s) 2500
BspMI ACCTGC 1 cut(s) 90
BspPI GGATC 5 cut(s) 438, 796, 1070, 1258, 2271
BspQI GCTCTTC 1 cut(s) 1730
BspT104I TTCGAA 1 cut(s) 1417
BsrDI GCAATG 1 cut(s) 1523
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 6 cut(s) 883, 1177, 1185, 1685, 2263, 2394
BssECI CCNNGG 8 cut(s) 157, 924, 964, 965, 1126, 1803, 1862, 1968
BssMI GATC 9 cut(s) 184, 430, 788, 1075, 1250, 1848, 2271, 2276, 2611
BssT1I CCWWGG 5 cut(s) 157, 1126, 1803, 1862, 1968
Bst2UI CCWGG 2 cut(s) 831, 966
Bst4CI ACNGT 6 cut(s) 717, 808, 1090, 1194, 1227, 2548
Bst6I CTCTTC 3 cut(s) 1289, 1730, 2067
BstAPI GCANNNNNTGC 3 cut(s) 42, 2495, 2504
BstAUI TGTACA 1 cut(s) 174
BstBI TTCGAA 1 cut(s) 1417
BstC8I GCNNGC 1 cut(s) 994
BstDSI CCRYGG 2 cut(s) 157, 1862
BstEII GGTNACC 2 cut(s) 825, 1841
BstF5I GGATG 5 cut(s) 394, 451, 1813, 1851, 2166
BstHHI GCGC 1 cut(s) 1373
BstKTI GATC 9 cut(s) 187, 433, 791, 1078, 1253, 1851, 2274, 2279, 2614
BstMAI GTCTC 7 cut(s) 510, 582, 688, 1042, 1216, 2474, 2521
BstMBI GATC 9 cut(s) 184, 430, 788, 1075, 1250, 1848, 2271, 2276, 2611
BstMWI GCNNNNNNNGC 9 cut(s) 42, 254, 407, 1589, 1775, 2375, 2495, 2501, 2504
BstNI CCWGG 2 cut(s) 831, 966
BstNSI RCATGY 3 cut(s) 610, 1823, 2186
BstPI GGTNACC 2 cut(s) 825, 1841
BstSCI CCNGG 4 cut(s) 829, 923, 964, 2551
BstSFI CTRYAG 1 cut(s) 2496
BstV1I GCAGC 4 cut(s) 2479, 2482, 2491, 2501
BstX2I RGATCY 3 cut(s) 430, 1250, 2276
BstYI RGATCY 3 cut(s) 430, 1250, 2276
Bsu15I ATCGAT 1 cut(s) 2614
BsuI GTATCC 1 cut(s) 569
BsuRI GGCC 3 cut(s) 756, 1165, 1964
BsuTUI ATCGAT 1 cut(s) 2614
BtgI CCRYGG 2 cut(s) 157, 1862
BtgZI GCGATG 1 cut(s) 792
BtsCI GGATG 5 cut(s) 394, 451, 1813, 1851, 2166
BtsI GCAGTG 2 cut(s) 1405, 2117
BtsIMutI CAGTG 6 cut(s) 85, 722, 1405, 2117, 2387, 2518
BveI ACCTGC 1 cut(s) 90
Cac8I GCNNGC 1 cut(s) 994
CaiI CAGNNNCTG 2 cut(s) 2495, 2504
CfoI GCGC 1 cut(s) 1373
Cfr13I GGNCC 5 cut(s) 102, 720, 865, 1164, 2599
ClaI ATCGAT 1 cut(s) 2614
Csp6I GTAC 7 cut(s) 175, 861, 1155, 1228, 2061, 2544, 2549
CviQI GTAC 7 cut(s) 175, 861, 1155, 1228, 2061, 2544, 2549
DpnI GATC 9 cut(s) 186, 432, 790, 1077, 1252, 1850, 2273, 2278, 2613
DpnII GATC 9 cut(s) 184, 430, 788, 1075, 1250, 1848, 2271, 2276, 2611
DraI TTTAAA 2 cut(s) 1383, 2584
DraIII CACNNNGTG 1 cut(s) 2513
DriI GACNNNNNGTC 1 cut(s) 701
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 3 cut(s) 1289, 1730, 2067
Eam1105I GACNNNNNGTC 1 cut(s) 701
EarI CTCTTC 3 cut(s) 1289, 1730, 2067
Eco130I CCWWGG 5 cut(s) 157, 1126, 1803, 1862, 1968
Eco147I AGGCCT 1 cut(s) 1964
Eco24I GRGCYC 1 cut(s) 1742
Eco32I GATATC 1 cut(s) 1110
Eco47I GGWCC 4 cut(s) 102, 720, 865, 2599
Eco57I CTGAAG 5 cut(s) 61, 417, 1547, 1754, 2091
Eco88I CYCGRG 1 cut(s) 92
Eco91I GGTNACC 2 cut(s) 825, 1841
EcoO109I RGGNCCY 1 cut(s) 102
EcoO65I GGTNACC 2 cut(s) 825, 1841
EcoRI GAATTC 1 cut(s) 2086
EcoRII CCWGG 2 cut(s) 829, 964
EcoRV GATATC 1 cut(s) 1110
EcoT14I CCWWGG 5 cut(s) 157, 1126, 1803, 1862, 1968
EcoT22I ATGCAT 2 cut(s) 47, 1669
EcoT38I GRGCYC 1 cut(s) 1742
ErhI CCWWGG 5 cut(s) 157, 1126, 1803, 1862, 1968
FaqI GGGAC 3 cut(s) 88, 931, 997
FbaI TGATCA 1 cut(s) 1848
Fnu4HI GCNGC 4 cut(s) 2490, 2493, 2496, 2505
FokI GGATG 5 cut(s) 401, 458, 1820, 1838, 2153
FriOI GRGCYC 1 cut(s) 1742
Fsp4HI GCNGC 4 cut(s) 2490, 2493, 2496, 2505
FspBI CTAG 3 cut(s) 107, 119, 251
GlaI GCGC 1 cut(s) 1372
GluI GCNGC 4 cut(s) 2490, 2493, 2496, 2505
GsaI CCCAGC 1 cut(s) 1778
GsuI CTGGAG 4 cut(s) 471, 1660, 1668, 2280
HaeIII GGCC 3 cut(s) 756, 1165, 1964
HapII CCGG 3 cut(s) 924, 1055, 2553
HhaI GCGC 1 cut(s) 1373
Hin6I GCGC 1 cut(s) 1371
HinP1I GCGC 1 cut(s) 1371
HincII GTYRAC 2 cut(s) 1123, 1190
HindII GTYRAC 2 cut(s) 1123, 1190
HindIII AAGCTT 1 cut(s) 639
HpaII CCGG 3 cut(s) 924, 1055, 2553
HphI GGTGA 6 cut(s) 248, 274, 819, 862, 1072, 2311
Hpy166II GTNNAC 5 cut(s) 177, 723, 1123, 1190, 1489
Hpy8I GTNNAC 5 cut(s) 177, 723, 1123, 1190, 1489
HpyAV CCTTC 1 cut(s) 1384
HpyCH4III ACNGT 6 cut(s) 717, 808, 1090, 1194, 1227, 2548
HpyCH4IV ACGT 2 cut(s) 1010, 1925
HpyF10VI GCNNNNNNNGC 9 cut(s) 42, 254, 407, 1589, 1775, 2375, 2495, 2501, 2504
HpySE526I ACGT 2 cut(s) 1010, 1925
HspAI GCGC 1 cut(s) 1371
Ksp22I TGATCA 1 cut(s) 1848
Kzo9I GATC 9 cut(s) 184, 430, 788, 1075, 1250, 1848, 2271, 2276, 2611
LguI GCTCTTC 1 cut(s) 1730
LmnI GCTCC 4 cut(s) 167, 563, 1351, 2315
Lsp1109I GCAGC 4 cut(s) 2479, 2482, 2491, 2501
MaeI CTAG 3 cut(s) 107, 119, 251
MaeII ACGT 2 cut(s) 1010, 1925
MaeIII GTNAC 7 cut(s) 197, 669, 825, 903, 1465, 1841, 2017
MalI GATC 9 cut(s) 186, 432, 790, 1077, 1252, 1850, 2273, 2278, 2613
MboI GATC 9 cut(s) 184, 430, 788, 1075, 1250, 1848, 2271, 2276, 2611
MboII GAAGA 8 cut(s) 40, 503, 806, 1276, 1747, 1968, 2084, 2542
MfeI CAATTG 3 cut(s) 509, 1884, 2013
MflI RGATCY 3 cut(s) 430, 1250, 2276
MhlI GDGCHC 3 cut(s) 535, 1348, 1742
MlsI TGGCCA 1 cut(s) 756
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 3 cut(s) 221, 1036, 1889
MmeI TCCRAC 1 cut(s) 460
Mox20I TGGCCA 1 cut(s) 756
Mph1103I ATGCAT 2 cut(s) 47, 1669
MroXI GAANNNNTTC 2 cut(s) 2090, 2441
MscI TGGCCA 1 cut(s) 756
MslI CAYNNNNRTG 2 cut(s) 981, 1694
Msp20I TGGCCA 1 cut(s) 756
MspA1I CMGCKG 2 cut(s) 1151, 2492
MspI CCGG 3 cut(s) 924, 1055, 2553
MspR9I CCNGG 4 cut(s) 831, 925, 966, 2553
MunI CAATTG 3 cut(s) 509, 1884, 2013
Mva1269I GAATGC 2 cut(s) 1273, 2411
MvaI CCWGG 2 cut(s) 831, 966
MwoI GCNNNNNNNGC 9 cut(s) 42, 254, 407, 1589, 1775, 2375, 2495, 2501, 2504
NciI CCSGG 2 cut(s) 925, 2553
NcoI CCATGG 2 cut(s) 157, 1862
NdeII GATC 9 cut(s) 184, 430, 788, 1075, 1250, 1848, 2271, 2276, 2611
NlaIV GGNNCC 3 cut(s) 104, 163, 559
NmuCI GTSAC 2 cut(s) 825, 1465
NsiI ATGCAT 2 cut(s) 47, 1669
NspI RCATGY 3 cut(s) 610, 1823, 2186
NspV TTCGAA 1 cut(s) 1417
PaeR7I CTCGAG 1 cut(s) 92
PasI CCCWGGG 1 cut(s) 965
PceI AGGCCT 1 cut(s) 1964
PciI ACATGT 2 cut(s) 606, 1819
PciSI GCTCTTC 1 cut(s) 1730
PctI GAATGC 2 cut(s) 1273, 2411
PdmI GAANNNNTTC 2 cut(s) 2090, 2441
Pfl23II CGTACG 1 cut(s) 860
PflMI CCANNNNNTGG 2 cut(s) 914, 1151
PkrI GCNGC 4 cut(s) 2491, 2494, 2497, 2506
PleI GAGTC 3 cut(s) 220, 1035, 1888
PpsI GAGTC 3 cut(s) 220, 1035, 1888
PpuMI RGGWCCY 1 cut(s) 102
PscI ACATGT 2 cut(s) 606, 1819
PsiI TTATAA 3 cut(s) 732, 1425, 1658
Psp5II RGGWCCY 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 829, 964
PspEI GGTNACC 2 cut(s) 825, 1841
PspFI CCCAGC 1 cut(s) 1774
PspGI CCWGG 2 cut(s) 829, 964
PspLI CGTACG 1 cut(s) 860
PspN4I GGNNCC 3 cut(s) 104, 163, 559
PspPI GGNCC 5 cut(s) 102, 720, 865, 1164, 2599
PspPPI RGGWCCY 1 cut(s) 102
PstI CTGCAG 1 cut(s) 2500
PstNI CAGNNNCTG 2 cut(s) 2495, 2504
PsuI RGATCY 3 cut(s) 430, 1250, 2276
PvuII CAGCTG 2 cut(s) 1151, 2492
RsaI GTAC 7 cut(s) 176, 862, 1156, 1229, 2062, 2545, 2550
RsaNI GTAC 7 cut(s) 175, 861, 1155, 1228, 2061, 2544, 2549
RseI CAYNNNNRTG 2 cut(s) 981, 1694
SapI GCTCTTC 1 cut(s) 1730
SatI GCNGC 4 cut(s) 2490, 2493, 2496, 2505
Sau3AI GATC 9 cut(s) 184, 430, 788, 1075, 1250, 1848, 2271, 2276, 2611
Sau96I GGNCC 5 cut(s) 102, 720, 865, 1164, 2599
SchI GAGTC 3 cut(s) 221, 1036, 1889
ScrFI CCNGG 4 cut(s) 831, 925, 966, 2553
SduI GDGCHC 3 cut(s) 535, 1348, 1742
SfcI CTRYAG 1 cut(s) 2496
Sfr274I CTCGAG 1 cut(s) 92
SfuI TTCGAA 1 cut(s) 1417
SinI GGWCC 4 cut(s) 102, 720, 865, 2599
SlaI CTCGAG 1 cut(s) 92
SmiMI CAYNNNNRTG 2 cut(s) 981, 1694
SmlI CTYRAG 2 cut(s) 92, 1500
SmoI CTYRAG 2 cut(s) 92, 1500
SseBI AGGCCT 1 cut(s) 1964
SsiI CCGC 3 cut(s) 141, 1619, 2122
SspI AATATT 1 cut(s) 2559
SspMI CTAG 3 cut(s) 107, 119, 251
StuI AGGCCT 1 cut(s) 1964
StyD4I CCNGG 4 cut(s) 829, 923, 964, 2551
StyI CCWWGG 5 cut(s) 157, 1126, 1803, 1862, 1968
TaaI ACNGT 6 cut(s) 717, 808, 1090, 1194, 1227, 2548
TaiI ACGT 2 cut(s) 1013, 1928
TaqI TCGA 4 cut(s) 93, 187, 1417, 2614
TatI WGTACW 2 cut(s) 174, 2060
TscAI CASTG 6 cut(s) 85, 722, 1405, 2124, 2394, 2518
TseFI GTSAC 2 cut(s) 825, 1465
TseI GCWGC 4 cut(s) 2489, 2492, 2495, 2504
Tsp45I GTSAC 2 cut(s) 825, 1465
TspGWI ACGGA 2 cut(s) 613, 878
TspRI CASTG 6 cut(s) 85, 722, 1405, 2124, 2394, 2518
Van91I CCANNNNNTGG 2 cut(s) 914, 1151
VpaK11BI GGWCC 4 cut(s) 102, 720, 865, 2599
XapI RAATTY 8 cut(s) 319, 841, 1419, 1454, 1543, 1717, 2023, 2086
XbaI TCTAGA 1 cut(s) 118
XceI RCATGY 3 cut(s) 610, 1823, 2186
XcmI CCANNNNNNNNNTGG 2 cut(s) 443, 568
XhoI CTCGAG 1 cut(s) 92
XmnI GAANNNNTTC 2 cut(s) 2090, 2441
XspI CTAG 3 cut(s) 107, 119, 251
Zsp2I ATGCAT 2 cut(s) 47, 1669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.