Rmu_sc0000766.1_g000011

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000766.1
OG0002511
Physical Location & Seq
Forward (+)
42559 .. 51062
8504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000766.1_g000011.1.cds

Sequence Viewer

Length: 3219 bp
atgttcgccaagcgactcttacagaaggccgtcaaccaccaccaccattctcagcacagtttgcaacaagaaagtatgacgtcagcagacttggacctgcgagttgcagctcactatggtattccatctacggcgtccattcttgctttcgaccccattcagcggcttctggccatcgggacattggatgggaggattaaagtaattggtggtgacggtattgaaggacttcttatatcccaaaaacaattgccttacaaatacatcgagtttctacataaccagggttacttggtcagcatcttaaatgataataacattgaggtttggaatcttgagggcagatgcctagtttgttctctagagtgggaatcaaatataactgctttttctgtaatccatggctcgaacttaatgtatgtgggcgatgagtatgctctattggctgtggtgaagtatgatgctgacgagggaaaacttttgcagttgccatatcatatatcttcagattcattaagcgaagctgccgagtttccatttcctaccgaccaaccgatagttggacttcttccccaacctggttcatctggcaatagagtactgattgcgtatcaaaatgggttgcttattctttgggatgtgtccgaggctcaaattgtttttattggaggtggtaaggatctccaactaaaggatggagttgttaagcctactgatgaagtcaatatagattctccagaaaatacaatagaacatcaactgggagagaaagagataagtgctctttgttgggcatcatccaatgggtccattctggctgtgggatatgtagatggagatattctgttctggaacacgtcatctgctgcttccattaagggtcagcaagcattgtcatcatctaataatgttgttaagttacgattatcatcatcagaaagaagacttcctgttattgtcttacagtggtccacattaaataaatcccataatgattctgatggacaactatttatctatggtggcgatgaaataggatcagacgaagttctgacggttttgactcttgattggtcttctgggatggggaatttaagatgtgttggccgtacagaccttacccttactggttcgtttgcagacatgattttattaccaaattttgcaacaattgcgggtaatcacagagctgatgcatttgtgttaacaaaccctgggcaattgcacttttatgatgaaaccagtctctcagccctaatatcccagcaagaaaagaaaccatctgtctctgctttggagtttccagtggtgatacctaccactaatccgaccatgactgctgccagacttattaggacagctactggggagaactcgttaaagtctctatcagagttatgctcagttgtgaaccttggctcactaccaacttcattagatggtacaaaatggcccttgactgggggtgtgcccagtcaattgtctctttcgaagagtagtggtattgagagagtatatctagcaggatattcagatggatctgttcggatctggaatgccacctatccactcttatcttttatctctgttttagaaggcgaggtgcaagatataaaagtggctggtttaagttctccggtatcaagaatggatttctgcagcttcactttaaacttggctgttggcagtgaatctggtttggttcgaatttataacctcaaagggcattcagatggggtaaaattcctctttgtgaccgaaaccaaatgtgaagcccacagtttgtctcaaggaaaaggacctcaatgcagagctgttttcagtcttactaattcccgagttcaagcacttcagtttgtgaagcatggaggtaaacttgcagtgggatttgattgcggttatgttgcaatgctggacacaaattccttatctatattgttcttcataaaagatgcatatttctctagctcctcagtcatttcattgacttggaaagaaattacaaatcctcaaggcctcttgaagagttcaaagctctcggaaactaaatcaccagttcaccctgcagaggaagtaatgttcattctaaccaaagatgcaaatatacatttaatttgtggaaacacgggtaatatggtcatccctcggccatggcacttgaaaaaggagtcgattgcaatttcaatgtatgttatagatggcagaatttcagcctctaaagtatctgatgcaaatccgccggaagaggcctcaaaggatacttcaaccaagaatgagtctatggccggcagttctccaattcctatagattcacttgaggtggaccaggaccataatacagaaaatgcatactctgaagaaagattgttgaattcactgattttgctttgttgtgtggattcagtgcaattgtactccacaaaatctgtgattcagggaaacaatgaacccattcgaaaggtgaaacatgcaagaccttgcatttggacggcaactttgaaaaaagttgaaaaagtttggggattgactttgctgtttcagactggagagattgaaattagatctattcctgatttagaattggtgaaagaaagctccttaatgtcaattctaaggtggaattgtaaggcaaatatggataagacaatgagttccgatgatgcacatatcacactggcaaatgggtatgaaacggtattcatctctctgttagctgcggaaaatgatttcaggattccagagtctttgccttgtctccatgatgcagttcttgcagctgctgcagatgctgcattaagtgcctctttaaatcagaagaagaaacagagtactgcccctgcaatattaggtggtattgtcaaaggcttgaaaggaggaaaaatggttcagagtggagattctactgcaactcctaaatccaggttcgatcatttagaaggcatgttttgcaagtcccagcagtgggactcggtcccagctttggatcatcaagaaaatctggagcttaatatagatgacattgaaattgatgaacctctacgcgtgacatctacttcaacatcccatgatgatgataataaaaggagagaaggggactcagaaagggatagattatttcaaggtgggactagtgatacaaagcccagagttaggactgctgaagaagggaggatctacagaatggagctgaagattttgcatcaatggcgaatgagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000137 GO:0000139 GO:0000902 GO:0000904 GO:0002791 GO:0003674 GO:0005096 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005795 GO:0005802 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006892 GO:0006893 GO:0006996 GO:0007010 GO:0007275 GO:0007399 GO:0007409 GO:0008047 GO:0008150 GO:0009653 GO:0009987 GO:0010008 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0017016 GO:0017137 GO:0017157 GO:0019899 GO:0019900 GO:0019901 GO:0022008 GO:0022607 GO:0030029 GO:0030030 GO:0030036 GO:0030154 GO:0030182 GO:0030234 GO:0030695 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0031090 GO:0031175 GO:0031267 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0031985 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048193 GO:0048468 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050708 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051046 GO:0051049 GO:0051179 GO:0051223 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0060627 GO:0061564 GO:0065003 GO:0065007 GO:0065009 GO:0070201 GO:0071840 GO:0071944 GO:0090087 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0098876 GO:0099568 GO:0120036 GO:0120039 GO:1903530
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 Synaptobrevin

Protein Analysis

1072

Amino Acids

117.24

Weight (kDa)

5.45

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1713
AatII GACGTC 1 cut(s) 83
Acc36I ACCTGC 1 cut(s) 105
AccII CGCG 1 cut(s) 3042
AciI CCGC 5 cut(s) 163, 1175, 1896, 2237, 2717
AclWI GGATC 6 cut(s) 687, 1045, 1546, 1556, 2991, 3179
AcoI YGGCCR 4 cut(s) 171, 1105, 2147, 2283
AcsI RAATTY 7 cut(s) 1090, 1159, 1707, 1742, 1921, 2205, 2371
AcuI CTGAAG 5 cut(s) 489, 1835, 2376, 3180, 3209
AcyI GRCGYC 2 cut(s) 80, 134
AfaI GTAC 5 cut(s) 600, 1111, 1444, 2414, 2830
AflIII ACRYGT 2 cut(s) 855, 3040
AhlI ACTAGT 1 cut(s) 3128
AjiI CACGTC 1 cut(s) 858
AjnI CCWGG 5 cut(s) 282, 577, 1213, 2325, 2918
AloI GAACNNNNNNTCC 4 cut(s) 2063, 2095, 2868, 2900
Alw21I GWGCWC 1 cut(s) 784
Alw26I GTCTC 6 cut(s) 1250, 1291, 1389, 1488, 1791, 2759
AlwI GGATC 6 cut(s) 687, 1045, 1546, 1556, 2991, 3179
AlwNI CAGNNNCTG 3 cut(s) 1364, 2780, 2789
Ama87I CYCGRG 1 cut(s) 1836
AoxI GGCC 8 cut(s) 27, 171, 1105, 1451, 2014, 2147, 2247, 2283
ApoI RAATTY 7 cut(s) 1090, 1159, 1707, 1742, 1921, 2205, 2371
ArsI GACNNNNNNTTYG 4 cut(s) 2466, 2479, 2498, 2511
Asp700I GAANNNNTTC 2 cut(s) 228, 2451
AspS9I GGNCC 8 cut(s) 94, 807, 969, 1452, 1799, 2323, 2329, 2971
AsuHPI GGTGA 7 cut(s) 224, 463, 1321, 2043, 2051, 2473, 2596
AsuII TTCGAA 3 cut(s) 1490, 1705, 2455
AvaI CYCGRG 1 cut(s) 1836
AvaII GGWCC 7 cut(s) 94, 807, 969, 1799, 2323, 2329, 2971
BaeGI GKGCMC 1 cut(s) 1473
BalI TGGCCA 1 cut(s) 173
BbsI GAAGAC 2 cut(s) 949, 1068
Bbv12I GWGCWC 1 cut(s) 784
BceAI ACGGC 4 cut(s) 14, 147, 1092, 2505
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 5 cut(s) 284, 579, 1215, 2327, 2920
BciVI GTATCC 1 cut(s) 2251
BcoDI GTCTC 6 cut(s) 1250, 1291, 1389, 1488, 1791, 2759
BcuI ACTAGT 1 cut(s) 3128
BfaI CTAG 6 cut(s) 350, 362, 1520, 1965, 3129, 3217
BfmI CTRYAG 5 cut(s) 1657, 2064, 2304, 2781, 3175
BfuAI ACCTGC 1 cut(s) 105
BfuI GTATCC 1 cut(s) 2251
BglII AGATCT 1 cut(s) 2561
BmcAI AGTACT 2 cut(s) 600, 2830
Bme1390I CCNGG 5 cut(s) 284, 579, 1215, 2327, 2920
Bme18I GGWCC 7 cut(s) 94, 807, 969, 1799, 2323, 2329, 2971
BmeT110I CYCGRG 1 cut(s) 1836
BmgBI CACGTC 1 cut(s) 858
BmgT120I GGNCC 8 cut(s) 94, 807, 969, 1452, 1799, 2323, 2329, 2971
BmiI GGNNCC 2 cut(s) 808, 2973
BmrFI CCNGG 5 cut(s) 284, 579, 1215, 2327, 2920
BmrI ACTGGG 4 cut(s) 770, 1374, 1467, 1470
BmuI ACTGGG 4 cut(s) 770, 1374, 1467, 1470
BpiI GAAGAC 2 cut(s) 949, 1068
BplI GAGNNNNNCTC 2 cut(s) 1385, 1417
BpmI CTGGAG 3 cut(s) 720, 2565, 3020
Bpu14I TTCGAA 3 cut(s) 1490, 1705, 2455
BpuEI CTTGAG 4 cut(s) 356, 1773, 1995, 2336
BsaBI GATNNNNATC 2 cut(s) 928, 2232
BsaHI GRCGYC 2 cut(s) 80, 134
BsaJI CCNNGG 8 cut(s) 283, 400, 645, 1213, 1214, 1414, 2144, 2150
BsaWI WCCGGW 1 cut(s) 1636
BsaXI ACNNNNNCTCC 4 cut(s) 1361, 1391, 2893, 2923
Bse118I RCCGGY 1 cut(s) 2285
Bse3DI GCAATG 1 cut(s) 1914
Bse8I GATNNNNATC 2 cut(s) 928, 2232
BseBI CCWGG 5 cut(s) 284, 579, 1215, 2327, 2920
BseDI CCNNGG 8 cut(s) 283, 400, 645, 1213, 1214, 1414, 2144, 2150
BseGI GGATG 7 cut(s) 193, 643, 700, 797, 1089, 2139, 3059
BseJI GATNNNNATC 2 cut(s) 928, 2232
BseMI GCAATG 1 cut(s) 1914
BseMII CTCAG 5 cut(s) 65, 1263, 1416, 1986, 3111
BseRI GAGGAG 1 cut(s) 1960
BseSI GKGCMC 1 cut(s) 1473
BseYI CCCAGC 3 cut(s) 1263, 2955, 2974
Bsh1236I CGCG 1 cut(s) 3042
BshFI GGCC 8 cut(s) 29, 173, 1107, 1453, 2016, 2149, 2249, 2285
BsiHKAI GWGCWC 1 cut(s) 784
BsiHKCI CYCGRG 1 cut(s) 1836
BsiSI CCGG 3 cut(s) 1637, 2240, 2286
BslFI GGGAC 6 cut(s) 193, 2938, 2957, 2978, 3107, 3139
BsmAI GTCTC 6 cut(s) 1250, 1291, 1389, 1488, 1791, 2759
BsmFI GGGAC 6 cut(s) 193, 2938, 2957, 2978, 3107, 3139
BsmI GAATGC 2 cut(s) 1561, 1726
BsnI GGCC 8 cut(s) 29, 173, 1107, 1453, 2016, 2149, 2249, 2285
BsoBI CYCGRG 1 cut(s) 1836
Bsp119I TTCGAA 3 cut(s) 1490, 1705, 2455
Bsp1286I GDGCHC 2 cut(s) 784, 1473
Bsp143I GATC 8 cut(s) 679, 1037, 1538, 1548, 2561, 2926, 2983, 3171
Bsp19I CCATGG 2 cut(s) 400, 2150
BspACI CCGC 5 cut(s) 163, 1175, 1896, 2237, 2717
BspANI GGCC 8 cut(s) 29, 173, 1107, 1453, 2016, 2149, 2249, 2285
BspCNI CTCAG 5 cut(s) 64, 1262, 1415, 1985, 3110
BspFNI CGCG 1 cut(s) 3042
BspLI GGNNCC 2 cut(s) 808, 2973
BspMAI CTGCAG 3 cut(s) 1661, 2068, 2785
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 6 cut(s) 687, 1045, 1546, 1556, 2991, 3179
BspT104I TTCGAA 3 cut(s) 1490, 1705, 2455
BsrDI GCAATG 1 cut(s) 1914
BsrFI RCCGGY 1 cut(s) 2285
BssAI RCCGGY 1 cut(s) 2285
BssECI CCNNGG 8 cut(s) 283, 400, 645, 1213, 1214, 1414, 2144, 2150
BssMI GATC 8 cut(s) 679, 1037, 1538, 1548, 2561, 2926, 2983, 3171
BssNI GRCGYC 2 cut(s) 80, 134
BssT1I CCWWGG 3 cut(s) 400, 1414, 2150
Bst2UI CCWGG 5 cut(s) 284, 579, 1215, 2327, 2920
Bst4CI ACNGT 6 cut(s) 59, 218, 966, 1057, 1781, 2695
Bst6I CTCTTC 3 cut(s) 1487, 2018, 2238
BstACI GRCGYC 2 cut(s) 80, 134
BstAPI GCANNNNNTGC 7 cut(s) 61, 1172, 2771, 2780, 2789, 2798, 2946
BstBI TTCGAA 3 cut(s) 1490, 1705, 2455
BstC8I GCNNGC 2 cut(s) 888, 2287
BstDEI CTNAG 6 cut(s) 51, 1249, 1402, 1972, 2612, 3097
BstDSI CCRYGG 2 cut(s) 400, 2150
BstF5I GGATG 7 cut(s) 193, 643, 700, 797, 1089, 2139, 3059
BstFNI CGCG 1 cut(s) 3042
BstKTI GATC 8 cut(s) 682, 1040, 1541, 1551, 2564, 2929, 2986, 3174
BstMAI GTCTC 6 cut(s) 1250, 1291, 1389, 1488, 1791, 2759
BstMBI GATC 8 cut(s) 679, 1037, 1538, 1548, 2561, 2926, 2983, 3171
BstNI CCWGG 5 cut(s) 284, 579, 1215, 2327, 2920
BstNSI RCATGY 2 cut(s) 2471, 2944
BstSCI CCNGG 5 cut(s) 282, 577, 1213, 2325, 2918
BstSFI CTRYAG 5 cut(s) 1657, 2064, 2304, 2781, 3175
BstSLI GKGCMC 1 cut(s) 1473
BstUI CGCG 1 cut(s) 3042
BstV2I GAAGAC 2 cut(s) 949, 1068
BstX2I RGATCY 5 cut(s) 679, 1538, 1548, 2561, 3171
BstYI RGATCY 5 cut(s) 679, 1538, 1548, 2561, 3171
BsuI GTATCC 1 cut(s) 2251
BsuRI GGCC 8 cut(s) 29, 173, 1107, 1453, 2016, 2149, 2249, 2285
BtgI CCRYGG 2 cut(s) 400, 2150
BtgZI GCGATG 2 cut(s) 441, 1041
BtrI CACGTC 1 cut(s) 858
BtsCI GGATG 7 cut(s) 193, 643, 700, 797, 1089, 2139, 3059
BtsI GCAGTG 3 cut(s) 1693, 1887, 2966
BtsIMutI CAGTG 8 cut(s) 971, 1311, 1693, 1887, 2375, 2409, 2672, 2966
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 2 cut(s) 888, 2287
CaiI CAGNNNCTG 3 cut(s) 1364, 2780, 2789
Cfr10I RCCGGY 1 cut(s) 2285
Cfr13I GGNCC 8 cut(s) 94, 807, 969, 1452, 1799, 2323, 2329, 2971
CseI GACGC 1 cut(s) 123
CsiI ACCWGGT 1 cut(s) 577
Csp6I GTAC 5 cut(s) 599, 1110, 1443, 2413, 2829
CviAII CATG 9 cut(s) 401, 1144, 1333, 1865, 2151, 2468, 2759, 2941, 3065
CviQI GTAC 5 cut(s) 599, 1110, 1443, 2413, 2829
DdeI CTNAG 6 cut(s) 51, 1249, 1402, 1972, 2612, 3097
DpnI GATC 8 cut(s) 681, 1039, 1540, 1550, 2563, 2928, 2985, 3173
DpnII GATC 8 cut(s) 679, 1037, 1538, 1548, 2561, 2926, 2983, 3171
DraI TTTAAA 2 cut(s) 1671, 2808
EaeI YGGCCR 4 cut(s) 171, 1105, 2147, 2283
Eam1104I CTCTTC 3 cut(s) 1487, 2018, 2238
EarI CTCTTC 3 cut(s) 1487, 2018, 2238
EciI GGCGGA 1 cut(s) 2226
Eco130I CCWWGG 3 cut(s) 400, 1414, 2150
Eco147I AGGCCT 2 cut(s) 2016, 2249
Eco47I GGWCC 7 cut(s) 94, 807, 969, 1799, 2323, 2329, 2971
Eco57I CTGAAG 5 cut(s) 489, 1835, 2376, 3180, 3209
Eco88I CYCGRG 1 cut(s) 1836
EcoO109I RGGNCCY 1 cut(s) 1799
EcoRI GAATTC 1 cut(s) 2371
EcoRII CCWGG 5 cut(s) 282, 577, 1213, 2325, 2918
EcoT14I CCWWGG 3 cut(s) 400, 1414, 2150
EcoT22I ATGCAT 3 cut(s) 1198, 1957, 2350
ErhI CCWWGG 3 cut(s) 400, 1414, 2150
FaeI CATG 9 cut(s) 404, 1147, 1336, 1868, 2154, 2471, 2762, 2944, 3068
FalI AAGNNNNNCTT 5 cut(s) 34, 216, 248, 2788, 2820
FaqI GGGAC 6 cut(s) 193, 2938, 2957, 2978, 3107, 3139
FatI CATG 9 cut(s) 400, 1143, 1332, 1864, 2150, 2467, 2758, 2940, 3064
FauI CCCGC 1 cut(s) 1168
FokI GGATG 7 cut(s) 200, 650, 707, 784, 1096, 2126, 3046
FspBI CTAG 6 cut(s) 350, 362, 1520, 1965, 3129, 3217
GsaI CCCAGC 3 cut(s) 1267, 2959, 2978
GsuI CTGGAG 3 cut(s) 720, 2565, 3020
HaeIII GGCC 8 cut(s) 29, 173, 1107, 1453, 2016, 2149, 2249, 2285
HapII CCGG 3 cut(s) 1637, 2240, 2286
HgaI GACGC 1 cut(s) 123
Hin1I GRCGYC 2 cut(s) 80, 134
Hin1II CATG 9 cut(s) 404, 1147, 1336, 1868, 2154, 2471, 2762, 2944, 3068
HincII GTYRAC 2 cut(s) 34, 1206
HindII GTYRAC 2 cut(s) 34, 1206
HpaI GTTAAC 1 cut(s) 1206
HpaII CCGG 3 cut(s) 1637, 2240, 2286
HphI GGTGA 7 cut(s) 224, 463, 1321, 2043, 2051, 2473, 2596
Hpy166II GTNNAC 7 cut(s) 34, 972, 1206, 1411, 1874, 2059, 2323
Hpy8I GTNNAC 7 cut(s) 34, 972, 1206, 1411, 1874, 2059, 2323
HpyAV CCTTC 6 cut(s) 19, 218, 1589, 2930, 3083, 3158
HpyCH4III ACNGT 6 cut(s) 59, 218, 966, 1057, 1781, 2695
HpyCH4IV ACGT 2 cut(s) 80, 857
HpyF3I CTNAG 6 cut(s) 51, 1249, 1402, 1972, 2612, 3097
HpySE526I ACGT 2 cut(s) 80, 857
Hsp92I GRCGYC 2 cut(s) 80, 134
Hsp92II CATG 9 cut(s) 404, 1147, 1336, 1868, 2154, 2471, 2762, 2944, 3068
KroI GCCGGC 1 cut(s) 2285
KroNI GCCGGC 1 cut(s) 2287
KspAI GTTAAC 1 cut(s) 1206
Kzo9I GATC 8 cut(s) 679, 1037, 1538, 1548, 2561, 2926, 2983, 3171
LmnI GCTCC 4 cut(s) 1973, 2600, 3001, 3184
MabI ACCWGGT 1 cut(s) 577
MaeI CTAG 6 cut(s) 350, 362, 1520, 1965, 3129, 3217
MaeII ACGT 2 cut(s) 80, 857
MaeIII GTNAC 5 cut(s) 212, 287, 918, 1753, 3043
MalI GATC 8 cut(s) 681, 1039, 1540, 1550, 2563, 2928, 2985, 3173
MboI GATC 8 cut(s) 679, 1037, 1538, 1548, 2561, 2926, 2983, 3171
MfeI CAATTG 5 cut(s) 248, 1170, 1220, 1478, 2408
MflI RGATCY 5 cut(s) 679, 1538, 1548, 2561, 3171
MhlI GDGCHC 2 cut(s) 784, 1473
MlsI TGGCCA 1 cut(s) 173
MluI ACGCGT 1 cut(s) 3040
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 7 cut(s) 9, 1057, 2177, 2285, 2750, 2960, 3089
MmeI TCCRAC 3 cut(s) 541, 709, 1352
Mox20I TGGCCA 1 cut(s) 173
Mph1103I ATGCAT 3 cut(s) 1198, 1957, 2350
MroNI GCCGGC 1 cut(s) 2285
MroXI GAANNNNTTC 2 cut(s) 228, 2451
MscI TGGCCA 1 cut(s) 173
MslI CAYNNNNRTG 3 cut(s) 1230, 1731, 3069
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 2 cut(s) 163, 2777
MspI CCGG 3 cut(s) 1637, 2240, 2286
MspR9I CCNGG 5 cut(s) 284, 579, 1215, 2327, 2920
MunI CAATTG 5 cut(s) 248, 1170, 1220, 1478, 2408
Mva1269I GAATGC 2 cut(s) 1561, 1726
MvaI CCWGG 5 cut(s) 284, 579, 1215, 2327, 2920
MvnI CGCG 1 cut(s) 3042
NaeI GCCGGC 1 cut(s) 2287
NcoI CCATGG 2 cut(s) 400, 2150
NdeII GATC 8 cut(s) 679, 1037, 1538, 1548, 2561, 2926, 2983, 3171
NgoMIV GCCGGC 1 cut(s) 2285
NlaIII CATG 9 cut(s) 404, 1147, 1336, 1868, 2154, 2471, 2762, 2944, 3068
NlaIV GGNNCC 2 cut(s) 808, 2973
NmeAIII GCCGAG 2 cut(s) 553, 2125
NmuCI GTSAC 3 cut(s) 212, 1753, 3043
NsiI ATGCAT 3 cut(s) 1198, 1957, 2350
NspI RCATGY 2 cut(s) 2471, 2944
NspV TTCGAA 3 cut(s) 1490, 1705, 2455
PasI CCCWGGG 1 cut(s) 1214
PceI AGGCCT 2 cut(s) 2016, 2249
PctI GAATGC 2 cut(s) 1561, 1726
PdiI GCCGGC 1 cut(s) 2287
PdmI GAANNNNTTC 2 cut(s) 228, 2451
PflFI GACNNNGTC 1 cut(s) 2969
PleI GAGTC 7 cut(s) 9, 1057, 2176, 2284, 2749, 2960, 3089
PpsI GAGTC 7 cut(s) 9, 1057, 2176, 2284, 2749, 2960, 3089
PpuMI RGGWCCY 1 cut(s) 1799
PsiI TTATAA 1 cut(s) 1713
Psp5II RGGWCCY 1 cut(s) 1799
Psp6I CCWGG 5 cut(s) 282, 577, 1213, 2325, 2918
PspFI CCCAGC 3 cut(s) 1263, 2955, 2974
PspGI CCWGG 5 cut(s) 282, 577, 1213, 2325, 2918
PspN4I GGNNCC 2 cut(s) 808, 2973
PspPI GGNCC 8 cut(s) 94, 807, 969, 1452, 1799, 2323, 2329, 2971
PspPPI RGGWCCY 1 cut(s) 1799
PstI CTGCAG 3 cut(s) 1661, 2068, 2785
PstNI CAGNNNCTG 3 cut(s) 1364, 2780, 2789
PsuI RGATCY 5 cut(s) 679, 1538, 1548, 2561, 3171
PsyI GACNNNGTC 1 cut(s) 2969
PvuII CAGCTG 1 cut(s) 2777
RsaI GTAC 5 cut(s) 600, 1111, 1444, 2414, 2830
RsaNI GTAC 5 cut(s) 599, 1110, 1443, 2413, 2829
RseI CAYNNNNRTG 3 cut(s) 1230, 1731, 3069
Sau3AI GATC 8 cut(s) 679, 1037, 1538, 1548, 2561, 2926, 2983, 3171
Sau96I GGNCC 8 cut(s) 94, 807, 969, 1452, 1799, 2323, 2329, 2971
ScaI AGTACT 2 cut(s) 600, 2830
SchI GAGTC 7 cut(s) 9, 1057, 2177, 2285, 2750, 2960, 3089
ScrFI CCNGG 5 cut(s) 284, 579, 1215, 2327, 2920
SduI GDGCHC 2 cut(s) 784, 1473
SexAI ACCWGGT 1 cut(s) 577
SfcI CTRYAG 5 cut(s) 1657, 2064, 2304, 2781, 3175
SfuI TTCGAA 3 cut(s) 1490, 1705, 2455
SinI GGWCC 7 cut(s) 94, 807, 969, 1799, 2323, 2329, 2971
SmiMI CAYNNNNRTG 3 cut(s) 1230, 1731, 3069
SmlI CTYRAG 4 cut(s) 335, 1788, 2010, 2315
SmoI CTYRAG 4 cut(s) 335, 1788, 2010, 2315
SpeI ACTAGT 1 cut(s) 3128
SseBI AGGCCT 2 cut(s) 2016, 2249
SsiI CCGC 5 cut(s) 163, 1175, 1896, 2237, 2717
SspI AATATT 1 cut(s) 2844
SspMI CTAG 6 cut(s) 350, 362, 1520, 1965, 3129, 3217
StuI AGGCCT 2 cut(s) 2016, 2249
StyD4I CCNGG 5 cut(s) 282, 577, 1213, 2325, 2918
StyI CCWWGG 3 cut(s) 400, 1414, 2150
TaaI ACNGT 6 cut(s) 59, 218, 966, 1057, 1781, 2695
TaiI ACGT 2 cut(s) 83, 860
TaqI TCGA 8 cut(s) 150, 267, 407, 1490, 1705, 2171, 2455, 2925
TaqII GACCGA 2 cut(s) 1772, 2959
TatI WGTACW 3 cut(s) 598, 2412, 2828
TauI GCSGC 1 cut(s) 166
TscAI CASTG 8 cut(s) 971, 1311, 1693, 1887, 2382, 2409, 2679, 2966
TseFI GTSAC 3 cut(s) 212, 1753, 3043
Tsp45I GTSAC 3 cut(s) 212, 1753, 3043
TspRI CASTG 8 cut(s) 971, 1311, 1693, 1887, 2382, 2409, 2679, 2966
Tth111I GACNNNGTC 1 cut(s) 2969
VpaK11BI GGWCC 7 cut(s) 94, 807, 969, 1799, 2323, 2329, 2971
XapI RAATTY 7 cut(s) 1090, 1159, 1707, 1742, 1921, 2205, 2371
XbaI TCTAGA 1 cut(s) 361
XceI RCATGY 2 cut(s) 2471, 2944
XcmI CCANNNNNNNNNTGG 4 cut(s) 181, 557, 692, 817
XmnI GAANNNNTTC 2 cut(s) 228, 2451
XspI CTAG 6 cut(s) 350, 362, 1520, 1965, 3129, 3217
ZraI GACGTC 1 cut(s) 81
ZrmI AGTACT 2 cut(s) 600, 2830
Zsp2I ATGCAT 3 cut(s) 1198, 1957, 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.