Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003931

Physical Location & Seq

Genomic Coordinates Forward (+)

12968557 .. 12974058

Gene Structure 5502 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1164300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGGATCATGGCCGTACGCCTCTCGATTTCGACCTCAACGCCTTGCTATCCTACGCCTCCGCCAACGTTCATGGCTTTCCCCCCTCTCCCTCCAATTTCACTATCTCTAAGTTCGGGCATGGACAATCCAATCCGACTTATAAGGTAGAGACAGGATCGGGGGCGTCTCTGAAACGCTACGTTTTGAGGAAGAAGCCGCCGGGGAAGTTGCTCCAGTCTGCTCATGCCGTCGAGAGAGAGTTCCAGGTTCTTCAGGCATTGGGTACTCATACACTGGTTCCGGTTCCTAAAGTTTACTGTTTGTGTACCGATTCAAGAGTTATCGGAACCCCCTTTTACATCATGGAGTTTCTGGAAGGGCGCATTTTTCTCGACCCCAAGCTTCCGGGTGTAGCACCTGAGAGGAGGAGGCTACTTTACCAAGCAACTGCAAGAGCTTTAGCATCCCTACATTCTGCTGATGTTGATGCCATCGGTCTAGGAAAATATGGGCGTCGTAACAACTATTGTAAATGGCAGGTAGAGAGGTGGGCAAAGCAATATATTGCCTCAACTGGTGAGGGCAAGCCCAAGAGAAATCCGAAGATGTTTGAGCTGATTGATTGGTTGCAGCAGAATATTCCTCTTGAAGATTCTTCTGGGGCAGCAGCAGGCTTAGTTCATGGGGACTTTCGCATTGATAATCTTGTGTTTCATCCTATTGAGGATCGAGTGATTGGCATTCTCGACTGGGAATTATCTACTCTTGGAAATCAAATGTGTGATGTTGCTTATAGCTGTTTGCCTTACATCGTGGACATTGTGGCTGAAGTTGGTGAAGGTTTGGAACACACTGGGGTTCCAGAAGGGATTCCTTCTCAGGCAGAGTATGTAGCAGAATACTGTTCTTCATCGGGAAAGCCATGGCCTTTTGCTGAGTGGAAGTTCTATGTTACCTTTTCTTTATTTCGCGGGGCATCAATTTTTGCAGGGATATATAGTCGATGGATTATGGGTAATGCTTCTGGAGGAGAGAGTGCCCAGTATGCTGGGGACAGAGCAAATTTTATGATAGACTACGCATGGAAATTTATTGGACGAGAATCTGTGCTTCCCAAAAATCCTTCATCAGGTGCTTTCGTTGCTCAAGACTACTTGAAAAGATTTGGACAAGAGAGTGATGATCAAGGATTTTCAAAAGGGGGAGGGAGGTTTGTCCCCAGTAAAAGGGTTATGGAATTGAGAAACAGGTTGACAAAGTTCATGGAAGATCACATTTATCCCATGGAACAAGAATTCTACAAACTTGCTGAGTCTAACTCACGTTGGATGGTTCATCCAGGGGAGGAGAGGTTAAAGGAGCTAGCAAAGAAAGAAGGTCTCTGGAACTTATTTATACCTTTTGATAGTGCTGCCAGGGCCAGAAAATTAATTTTTGATGGAAGCAATCATCTTCTATCGGAGAATCCCTCCAATCGCTTATTGGGCGCAGGTCTTACAAACCTTGAATATGGATATCTTTGTGAGATCATGGGTCATTCAGTTTGGGCTCCGCAAGTGTTCAATTGTGGTGCACCTGACACAGGAAATATGGAGGTATTGTTGCGCTATGGCAGCAAGGAGCAGCTACTTGAATGGCTTGTTCCATTGCTTGAGGGGAAAATCCGGTCAGGATTTGCAATGACGGAACCCCAAGTTGCATCCTCTGATGCAACCAATATTGAGTGTTCTATTAAAAGACAAGGAGATTCATACATCATCAACGGAAAGAAATGGTGGACAAGTGGGGCTATGGATCCGAGGTGTAGAGTTCTTATAGTCATGGGGAAAACCGACTTCAACGCTTCAATGCATAAGCAGCAATCTATGATCTTAGTGGATATTCGAACTCCAGGTGTACACATAAAGAGACCACTTACGGTTTTTGGCTTTGATGATGCACCTCATGGGCATGCAGAAGTATTATTTGAGAATGTACGTGTCCCAGAAAAAAATATTCTACTGGGAGAAGGACGCGGGTTTGAAATCGCACAGGGTAGACTAGGCCCAGGAAGGTTACACCATTGCATGAGACTGATAGGAGCTGCCGAGCGTGGGATGCAGCTAATGGCTCAAAGGGCTCTTAGTAGAAAAGTGTTCGGAAAGTTGATTGCTGAACAAGGCTCATTTCGTTCAGACATTGCAAAGTGTCGAATTGAGCTAGAGAAAGCCAGATTGTTGGTTCTGGAGGCAGCTGACCAGCTTGATAGACTTGGAAACAAAAGGGCCCGAGGAACTATGGCAATGGCAAAGGTAGCAGCCCCAAACATGGCGCTTAAGGTACTTGACATGGCAATGCAAGTGCACGGTGGTGCTGGCTTGTCTTCCGACACTTGTCTGGCGCATCTTTGGGCCACAGCAAGAACGTTGAGAATCGCAGATGGTCCAGATGAAGTCCACTTGGGTACTATTGCCAAGTTAGAACTACGGAGAGCCAAACTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003995 GO:0005488 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016042 GO:0016049 GO:0016054 GO:0016491 GO:0016627 GO:0019395 GO:0019752 GO:0021700 GO:0022622 GO:0030154 GO:0030258 GO:0032501 GO:0032502 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0040007 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0046395 GO:0048037 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050660 GO:0050662 GO:0055114 GO:0060560 GO:0071695 GO:0071704 GO:0072329 GO:0080147 GO:0090558 GO:0090627 GO:0097159 GO:0099402 GO:1901265 GO:1901363 GO:1901575 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko00071 ko00280 ko00410 ko00640 ko01100 ko01110 ko01130 ko01200 ko01212 ko03320 map00071 map00280 map00410 map00640 map01100 map01110 map01130 map01200 map01212 map03320

Pfam Domains

Protein Families

APH Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

821

Amino Acids

91.32

Weight (kDa)

8.79

Isoelectric Point (pI)

41.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APH	PF01636	34 - 276	1.1e-48	Phosphotransferase enzyme family
Acyl-CoA_dh_M	PF02770	551 - 652	4.1e-18	Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1	PF00441	664 - 812	1e-36	Acyl-CoA dehydrogenase, C-terminal domain
Acyl-CoA_dh_2	PF08028	680 - 790	3.1e-08	Acyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06810
fragaria_vesca	FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700
malus_domestica	MD13G1164300.v1.1 MD16G1164100.v1.1
prunus_persica	Prupe.1G131800_v2.0.a1 Prupe.1G131800_v2.0.a1 Prupe.1G131800_v2.0.a1
pyrus_communis	pycom13g14030 pycom16g13880 pycom16g13890
rosa_chinensis	RchiOBHm_Chr3g0480631 RchiOBHm_Chr4g0423401 RchiOBHm_Chr6g0311831
rosa_laevigata	RLG00000007561
rosa_multiflora	Rmu_sc0002752.1_g000004 Rmu_ssc0000174.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365600
rosa_rugosa	Rorug04G0187300
rosa_samantha	Rh1BG304700 Rh3AG236600 Rh3DG262000 Rh4AG246200 Rh4AG246300 Rh4BG251700 Rh4CG260200 Rh4DG247100
rosa_wichuraiana	Rw4G021160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	141
Acc36I	ACCTGC	2 cut(s)	508, 1460
AccI	GTMKAC	1 cut(s)	2017
AccII	CGCG	2 cut(s)	951, 1995
AciI	CCGC	5 cut(s)	60, 197, 951, 1532, 1995
AclI	AACGTT	2 cut(s)	66, 2384
AclWI	GGATC	5 cut(s)	12, 163, 714, 1769, 1782
AcoI	YGGCCR	1 cut(s)	10
AcsI	RAATTY	3 cut(s)	1042, 1067, 1274
AcuI	CTGAAG	2 cut(s)	236, 828
AcyI	GRCGYC	2 cut(s)	164, 493
AfaI	GTAC	7 cut(s)	16, 265, 307, 1878, 1956, 2301, 2425
AfiI	CCNNNNNNNGG	5 cut(s)	1109, 1206, 1562, 2073, 2287
AflII	CTTAAG	1 cut(s)	2294
AflIII	ACRYGT	1 cut(s)	1957
AjnI	CCWGG	5 cut(s)	243, 1318, 1394, 1870, 2026
AleI	CACNNNNGTG	1 cut(s)	2328
Alw21I	GWGCWC	2 cut(s)	1555, 2325
Alw26I	GTCTC	5 cut(s)	143, 171, 1364, 1882, 2044
Alw44I	GTGCAC	2 cut(s)	1551, 2321
AlwI	GGATC	5 cut(s)	12, 163, 714, 1769, 1782
Ama87I	CYCGRG	1 cut(s)	2247
AoxI	GGCC	6 cut(s)	10, 905, 1398, 2023, 2244, 2370
ApaI	GGGCCC	1 cut(s)	2248
ApaLI	GTGCAC	2 cut(s)	1551, 2321
ApoI	RAATTY	3 cut(s)	1042, 1067, 1274
AseI	ATTAAT	1 cut(s)	1409
Asp700I	GAANNNNTTC	1 cut(s)	849
AspLEI	GCGC	5 cut(s)	363, 1469, 1587, 2293, 2362
AspS9I	GGNCC	6 cut(s)	1398, 2024, 2244, 2245, 2370, 2402
AsuC2I	CCSGG	2 cut(s)	201, 387
AsuHPI	GGTGA	2 cut(s)	569, 827
AsuII	TTCGAA	1 cut(s)	1864
AsuNHI	GCTAGC	1 cut(s)	1342
AvaI	CYCGRG	1 cut(s)	2247
AvaII	GGWCC	1 cut(s)	2402
BaeGI	GKGCMC	4 cut(s)	1021, 1555, 2248, 2325
BamHI	GGATCC	1 cut(s)	1774
BanII	GRGCYC	3 cut(s)	1531, 2101, 2248
BbsI	GAAGAC	1 cut(s)	2334
Bbv12I	GWGCWC	2 cut(s)	1555, 2325
BccI	CCATC	5 cut(s)	479, 978, 1303, 1412, 2393
BceAI	ACGGC	1 cut(s)	212
BcgI	CGANNNNNNTGC	2 cut(s)	352, 386
BciT130I	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
BclI	TGATCA	1 cut(s)	1162
BcnI	CCSGG	2 cut(s)	201, 387
BcoDI	GTCTC	5 cut(s)	143, 171, 1364, 1882, 2044
BfaI	CTAG	4 cut(s)	479, 1343, 2021, 2180
BfoI	RGCGCY	1 cut(s)	2294
BfrI	CTTAAG	1 cut(s)	2294
BfuAI	ACCTGC	2 cut(s)	508, 1460
Bme1390I	CCNGG	7 cut(s)	201, 245, 387, 1320, 1396, 1872, 2028
Bme18I	GGWCC	1 cut(s)	2402
BmeT110I	CYCGRG	1 cut(s)	2247
BmgT120I	GGNCC	6 cut(s)	1398, 2024, 2244, 2245, 2370, 2402
BmiI	GGNNCC	8 cut(s)	279, 285, 328, 840, 1530, 1668, 1776, 2246
BmrFI	CCNGG	7 cut(s)	201, 245, 387, 1320, 1396, 1872, 2028
BmrI	ACTGGG	5 cut(s)	739, 843, 1015, 1194, 1991
BmsI	GCATC	8 cut(s)	452, 457, 965, 1678, 1688, 1906, 2067, 2371
BmtI	GCTAGC	1 cut(s)	1346
BmuI	ACTGGG	5 cut(s)	739, 843, 1015, 1194, 1991
BoxI	GACNNNNGTC	1 cut(s)	2352
BpiI	GAAGAC	1 cut(s)	2334
BplI	GAGNNNNNCTC	5 cut(s)	1283, 1315, 1433, 1465, 2442
BpmI	CTGGAG	4 cut(s)	197, 1026, 1854, 2225
Bpu14I	TTCGAA	1 cut(s)	1864
BpuEI	CTTGAG	2 cut(s)	1110, 1652
BpuMI	CCSGG	2 cut(s)	201, 387
BsaAI	YACGTR	1 cut(s)	1958
BsaHI	GRCGYC	2 cut(s)	164, 493
BsaI	GGTCTC	2 cut(s)	1364, 1882
BsaJI	CCNNGG	8 cut(s)	200, 902, 1263, 1319, 1395, 1778, 2026, 2248
BsaWI	WCCGGW	2 cut(s)	280, 1644
BsaXI	ACNNNNNCTCC	4 cut(s)	1316, 1346, 1565, 1595
Bsc4I	CCNNNNNNNGG	5 cut(s)	1109, 1206, 1562, 2073, 2287
Bse1I	ACTGG	8 cut(s)	214, 279, 559, 734, 838, 1021, 1200, 1986
Bse3DI	GCAATG	6 cut(s)	1625, 1665, 2041, 2157, 2268, 2319
BseBI	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
BseDI	CCNNGG	8 cut(s)	200, 902, 1263, 1319, 1395, 1778, 2026, 2248
BseGI	GGATG	6 cut(s)	443, 694, 1314, 1315, 1679, 2082
BseLI	CCNNNNNNNGG	5 cut(s)	1109, 1206, 1562, 2073, 2287
BseMI	GCAATG	6 cut(s)	1625, 1665, 2041, 2157, 2268, 2319
BseMII	CTCAG	4 cut(s)	390, 872, 906, 1281
BseNI	ACTGG	8 cut(s)	214, 279, 559, 734, 838, 1021, 1200, 1986
BseRI	GAGGAG	4 cut(s)	418, 421, 1023, 1340
BseSI	GKGCMC	4 cut(s)	1021, 1555, 2248, 2325
BseYI	CCCAGC	1 cut(s)	1028
Bsh1236I	CGCG	2 cut(s)	951, 1995
BshFI	GGCC	6 cut(s)	12, 907, 1400, 2025, 2246, 2372
BsiHKAI	GWGCWC	2 cut(s)	1555, 2325
BsiHKCI	CYCGRG	1 cut(s)	2247
BsiSI	CCGG	4 cut(s)	200, 281, 386, 1645
BsiWI	CGTACG	1 cut(s)	14
BslFI	GGGAC	4 cut(s)	680, 1046, 1181, 1946
BslI	CCNNNNNNNGG	5 cut(s)	1109, 1206, 1562, 2073, 2287
BsmAI	GTCTC	5 cut(s)	143, 171, 1364, 1882, 2044
BsmBI	CGTCTC	1 cut(s)	171
BsmFI	GGGAC	4 cut(s)	680, 1046, 1181, 1946
BsmI	GAATGC	1 cut(s)	720
BsnI	GGCC	6 cut(s)	12, 907, 1400, 2025, 2246, 2372
Bso31I	GGTCTC	2 cut(s)	1364, 1882
BsoBI	CYCGRG	1 cut(s)	2247
Bsp119I	TTCGAA	1 cut(s)	1864
Bsp120I	GGGCCC	1 cut(s)	2244
Bsp1286I	GDGCHC	6 cut(s)	1021, 1531, 1555, 2101, 2248, 2325
Bsp1407I	TGTACA	1 cut(s)	1876
Bsp143I	GATC	8 cut(s)	4, 155, 706, 1162, 1249, 1506, 1774, 1848
Bsp19I	CCATGG	2 cut(s)	902, 1263
BspACI	CCGC	5 cut(s)	60, 197, 951, 1532, 1995
BspANI	GGCC	6 cut(s)	12, 907, 1400, 2025, 2246, 2372
BspCNI	CTCAG	4 cut(s)	391, 871, 907, 1282
BspFNI	CGCG	2 cut(s)	951, 1995
BspLI	GGNNCC	8 cut(s)	279, 285, 328, 840, 1530, 1668, 1776, 2246
BspMI	ACCTGC	2 cut(s)	508, 1460
BspOI	GCTAGC	1 cut(s)	1346
BspPI	GGATC	5 cut(s)	12, 163, 714, 1769, 1782
BspT104I	TTCGAA	1 cut(s)	1864
BspTI	CTTAAG	1 cut(s)	2294
BspTNI	GGTCTC	2 cut(s)	1364, 1882
BsrDI	GCAATG	6 cut(s)	1625, 1665, 2041, 2157, 2268, 2319
BsrGI	TGTACA	1 cut(s)	1876
BsrI	ACTGG	8 cut(s)	214, 279, 559, 734, 838, 1021, 1200, 1986
BssECI	CCNNGG	8 cut(s)	200, 902, 1263, 1319, 1395, 1778, 2026, 2248
BssMI	GATC	8 cut(s)	4, 155, 706, 1162, 1249, 1506, 1774, 1848
BssNI	GRCGYC	2 cut(s)	164, 493
BssT1I	CCWWGG	2 cut(s)	902, 1263
Bst2UI	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
Bst4CI	ACNGT	4 cut(s)	299, 884, 1900, 2327
BstACI	GRCGYC	2 cut(s)	164, 493
BstAFI	CTTAAG	1 cut(s)	2294
BstAUI	TGTACA	1 cut(s)	1876
BstBAI	YACGTR	1 cut(s)	1958
BstBI	TTCGAA	1 cut(s)	1864
BstC8I	GCNNGC	5 cut(s)	566, 652, 1344, 1932, 2335
BstDEI	CTNAG	8 cut(s)	108, 399, 655, 858, 915, 1290, 1852, 2102
BstDSI	CCRYGG	2 cut(s)	902, 1263
BstENI	CCTNNNNNAGG	2 cut(s)	1107, 1560
BstF5I	GGATG	6 cut(s)	443, 694, 1314, 1315, 1679, 2082
BstFNI	CGCG	2 cut(s)	951, 1995
BstH2I	RGCGCY	1 cut(s)	2294
BstHHI	GCGC	5 cut(s)	363, 1469, 1587, 2293, 2362
BstKTI	GATC	8 cut(s)	7, 158, 709, 1165, 1252, 1509, 1777, 1851
BstMAI	GTCTC	5 cut(s)	143, 171, 1364, 1882, 2044
BstMBI	GATC	8 cut(s)	4, 155, 706, 1162, 1249, 1506, 1774, 1848
BstMWI	GCNNNNNNNGC	9 cut(s)	1025, 1121, 1397, 1464, 1593, 1837, 2077, 2096, 2273
BstNI	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
BstNSI	RCATGY	1 cut(s)	1934
BstPAI	GACNNNNGTC	1 cut(s)	2352
BstSCI	CCNGG	7 cut(s)	199, 243, 385, 1318, 1394, 1870, 2026
BstSLI	GKGCMC	4 cut(s)	1021, 1555, 2248, 2325
BstUI	CGCG	2 cut(s)	951, 1995
BstV2I	GAAGAC	1 cut(s)	2334
BstX2I	RGATCY	1 cut(s)	1774
BstXI	CCANNNNNNTGG	2 cut(s)	1028, 2197
BstYI	RGATCY	1 cut(s)	1774
BsuRI	GGCC	6 cut(s)	12, 907, 1400, 2025, 2246, 2372
BtgI	CCRYGG	2 cut(s)	902, 1263
BtsCI	GGATG	6 cut(s)	443, 694, 1314, 1315, 1679, 2082
BtsIMutI	CAGTG	2 cut(s)	272, 831
BveI	ACCTGC	2 cut(s)	508, 1460
Cac8I	GCNNGC	5 cut(s)	566, 652, 1344, 1932, 2335
CfoI	GCGC	5 cut(s)	363, 1469, 1587, 2293, 2362
Cfr13I	GGNCC	6 cut(s)	1398, 2024, 2244, 2245, 2370, 2402
CseI	GACGC	3 cut(s)	153, 482, 2001
Csp6I	GTAC	7 cut(s)	15, 264, 306, 1877, 1955, 2300, 2424
CviQI	GTAC	7 cut(s)	15, 264, 306, 1877, 1955, 2300, 2424
DdeI	CTNAG	8 cut(s)	108, 399, 655, 858, 915, 1290, 1852, 2102
DpnI	GATC	8 cut(s)	6, 157, 708, 1164, 1251, 1508, 1776, 1850
DpnII	GATC	8 cut(s)	4, 155, 706, 1162, 1249, 1506, 1774, 1848
EaeI	YGGCCR	1 cut(s)	10
EciI	GGCGGA	1 cut(s)	49
Eco130I	CCWWGG	2 cut(s)	902, 1263
Eco24I	GRGCYC	3 cut(s)	1531, 2101, 2248
Eco31I	GGTCTC	2 cut(s)	1364, 1882
Eco32I	GATATC	1 cut(s)	1496
Eco47I	GGWCC	1 cut(s)	2402
Eco57I	CTGAAG	2 cut(s)	236, 828
Eco88I	CYCGRG	1 cut(s)	2247
EcoNI	CCTNNNNNAGG	2 cut(s)	1107, 1560
EcoO109I	RGGNCCY	1 cut(s)	2244
EcoRI	GAATTC	1 cut(s)	1274
EcoRII	CCWGG	5 cut(s)	243, 1318, 1394, 1870, 2026
EcoRV	GATATC	1 cut(s)	1496
EcoT14I	CCWWGG	2 cut(s)	902, 1263
EcoT22I	ATGCAT	1 cut(s)	1833
EcoT38I	GRGCYC	3 cut(s)	1531, 2101, 2248
ErhI	CCWWGG	2 cut(s)	902, 1263
Esp3I	CGTCTC	1 cut(s)	171
FaqI	GGGAC	4 cut(s)	680, 1046, 1181, 1946
FauI	CCCGC	2 cut(s)	944, 1988
FbaI	TGATCA	1 cut(s)	1162
FblI	GTMKAC	1 cut(s)	2017
FokI	GGATG	6 cut(s)	430, 681, 1302, 1321, 1666, 2089
FriOI	GRGCYC	3 cut(s)	1531, 2101, 2248
FspBI	CTAG	4 cut(s)	479, 1343, 2021, 2180
GlaI	GCGC	5 cut(s)	362, 1468, 1586, 2292, 2361
GsaI	CCCAGC	1 cut(s)	1032
GsuI	CTGGAG	4 cut(s)	197, 1026, 1854, 2225
HaeII	RGCGCY	1 cut(s)	2294
HaeIII	GGCC	6 cut(s)	12, 907, 1400, 2025, 2246, 2372
HapII	CCGG	4 cut(s)	200, 281, 386, 1645
HgaI	GACGC	3 cut(s)	153, 482, 2001
HhaI	GCGC	5 cut(s)	363, 1469, 1587, 2293, 2362
Hin1I	GRCGYC	2 cut(s)	164, 493
Hin6I	GCGC	5 cut(s)	361, 1467, 1585, 2291, 2360
HinP1I	GCGC	5 cut(s)	361, 1467, 1585, 2291, 2360
HincII	GTYRAC	1 cut(s)	1233
HindII	GTYRAC	1 cut(s)	1233
HindIII	AAGCTT	1 cut(s)	380
HinfI	GANTC	8 cut(s)	311, 632, 850, 1082, 1292, 1444, 1727, 2391
HpaII	CCGG	4 cut(s)	200, 281, 386, 1645
HphI	GGTGA	2 cut(s)	569, 827
Hpy99I	CGWCG	2 cut(s)	233, 498
HpyAV	CCTTC	8 cut(s)	350, 812, 839, 864, 1113, 1349, 1982, 2025
HpyCH4III	ACNGT	4 cut(s)	299, 884, 1900, 2327
HpyCH4IV	ACGT	5 cut(s)	66, 180, 1303, 1957, 2384
HpyF10VI	GCNNNNNNNGC	9 cut(s)	1025, 1121, 1397, 1464, 1593, 1837, 2077, 2096, 2273
HpyF3I	CTNAG	8 cut(s)	108, 399, 655, 858, 915, 1290, 1852, 2102
HpySE526I	ACGT	5 cut(s)	66, 180, 1303, 1957, 2384
Hsp92I	GRCGYC	2 cut(s)	164, 493
HspAI	GCGC	5 cut(s)	361, 1467, 1585, 2291, 2360
Ksp22I	TGATCA	1 cut(s)	1162
Kzo9I	GATC	8 cut(s)	4, 155, 706, 1162, 1249, 1506, 1774, 1848
LmnI	GCTCC	5 cut(s)	216, 1339, 1534, 1600, 2060
LweI	GCATC	8 cut(s)	452, 457, 965, 1678, 1688, 1906, 2067, 2371
MaeI	CTAG	4 cut(s)	479, 1343, 2021, 2180
MaeII	ACGT	5 cut(s)	66, 180, 1303, 1957, 2384
MaeIII	GTNAC	3 cut(s)	497, 931, 2034
MalI	GATC	8 cut(s)	6, 157, 708, 1164, 1251, 1508, 1776, 1850
MboI	GATC	8 cut(s)	4, 155, 706, 1162, 1249, 1506, 1774, 1848
MboII	GAAGA	9 cut(s)	202, 242, 595, 627, 641, 879, 1259, 1424, 2334
MfeI	CAATTG	1 cut(s)	1543
MflI	RGATCY	1 cut(s)	1774
MhlI	GDGCHC	6 cut(s)	1021, 1531, 1555, 2101, 2248, 2325
MlyI	GAGTC	1 cut(s)	1301
MmeI	TCCRAC	3 cut(s)	158, 1286, 2370
Mph1103I	ATGCAT	1 cut(s)	1833
MroXI	GAANNNNTTC	1 cut(s)	849
MseI	TTAA	4 cut(s)	1334, 1409, 1713, 2295
MslI	CAYNNNNRTG	3 cut(s)	1929, 2312, 2328
MspA1I	CMGCKG	1 cut(s)	2213
MspCI	CTTAAG	1 cut(s)	2294
MspI	CCGG	4 cut(s)	200, 281, 386, 1645
MspR9I	CCNGG	7 cut(s)	201, 245, 387, 1320, 1396, 1872, 2028
MunI	CAATTG	1 cut(s)	1543
Mva1269I	GAATGC	1 cut(s)	720
MvaI	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
MvnI	CGCG	2 cut(s)	951, 1995
MwoI	GCNNNNNNNGC	9 cut(s)	1025, 1121, 1397, 1464, 1593, 1837, 2077, 2096, 2273
NciI	CCSGG	2 cut(s)	201, 387
NcoI	CCATGG	2 cut(s)	902, 1263
NdeII	GATC	8 cut(s)	4, 155, 706, 1162, 1249, 1506, 1774, 1848
NheI	GCTAGC	1 cut(s)	1342
NlaIV	GGNNCC	8 cut(s)	279, 285, 328, 840, 1530, 1668, 1776, 2246
NmeAIII	GCCGAG	1 cut(s)	2092
NsiI	ATGCAT	1 cut(s)	1833
NspI	RCATGY	1 cut(s)	1934
NspV	TTCGAA	1 cut(s)	1864
OliI	CACNNNNGTG	1 cut(s)	2328
PaeI	GCATGC	1 cut(s)	1934
PctI	GAATGC	1 cut(s)	720
PdmI	GAANNNNTTC	1 cut(s)	849
PfeI	GAWTC	7 cut(s)	311, 632, 850, 1082, 1444, 1727, 2391
Pfl23II	CGTACG	1 cut(s)	14
PleI	GAGTC	1 cut(s)	1300
PpsI	GAGTC	1 cut(s)	1300
Ppu21I	YACGTR	1 cut(s)	1958
PshAI	GACNNNNGTC	1 cut(s)	2352
PshBI	ATTAAT	1 cut(s)	1409
PsiI	TTATAA	1 cut(s)	141
Psp1406I	AACGTT	2 cut(s)	66, 2384
Psp6I	CCWGG	5 cut(s)	243, 1318, 1394, 1870, 2026
PspFI	CCCAGC	1 cut(s)	1028
PspGI	CCWGG	5 cut(s)	243, 1318, 1394, 1870, 2026
PspLI	CGTACG	1 cut(s)	14
PspN4I	GGNNCC	8 cut(s)	279, 285, 328, 840, 1530, 1668, 1776, 2246
PspOMI	GGGCCC	1 cut(s)	2244
PspPI	GGNCC	6 cut(s)	1398, 2024, 2244, 2245, 2370, 2402
PsuI	RGATCY	1 cut(s)	1774
PvuII	CAGCTG	1 cut(s)	2213
RsaI	GTAC	7 cut(s)	16, 265, 307, 1878, 1956, 2301, 2425
RsaNI	GTAC	7 cut(s)	15, 264, 306, 1877, 1955, 2300, 2424
RseI	CAYNNNNRTG	3 cut(s)	1929, 2312, 2328
SaqAI	TTAA	4 cut(s)	1334, 1409, 1713, 2295
Sau3AI	GATC	8 cut(s)	4, 155, 706, 1162, 1249, 1506, 1774, 1848
Sau96I	GGNCC	6 cut(s)	1398, 2024, 2244, 2245, 2370, 2402
SchI	GAGTC	1 cut(s)	1301
ScrFI	CCNGG	7 cut(s)	201, 245, 387, 1320, 1396, 1872, 2028
SduI	GDGCHC	6 cut(s)	1021, 1531, 1555, 2101, 2248, 2325
SfaNI	GCATC	8 cut(s)	452, 457, 965, 1678, 1688, 1906, 2067, 2371
SfuI	TTCGAA	1 cut(s)	1864
SinI	GGWCC	1 cut(s)	2402
SmiMI	CAYNNNNRTG	3 cut(s)	1929, 2312, 2328
SmlI	CTYRAG	3 cut(s)	1125, 1631, 2294
SmoI	CTYRAG	3 cut(s)	1125, 1631, 2294
SphI	GCATGC	1 cut(s)	1934
SsiI	CCGC	5 cut(s)	60, 197, 951, 1532, 1995
SspI	AATATT	3 cut(s)	619, 1699, 1975
SspMI	CTAG	4 cut(s)	479, 1343, 2021, 2180
StyD4I	CCNGG	7 cut(s)	199, 243, 385, 1318, 1394, 1870, 2026
StyI	CCWWGG	2 cut(s)	902, 1263
TaaI	ACNGT	4 cut(s)	299, 884, 1900, 2327
TaiI	ACGT	5 cut(s)	69, 183, 1306, 1960, 2387
TaqI	TCGA	9 cut(s)	24, 30, 231, 372, 709, 726, 982, 1864, 2170
TaqII	GACCGA	1 cut(s)	464
TatI	WGTACW	1 cut(s)	1876
TauI	GCSGC	1 cut(s)	199
TfiI	GAWTC	7 cut(s)	311, 632, 850, 1082, 1444, 1727, 2391
Tru1I	TTAA	4 cut(s)	1334, 1409, 1713, 2295
Tru9I	TTAA	4 cut(s)	1334, 1409, 1713, 2295
TscAI	CASTG	2 cut(s)	279, 838
TspDTI	ATGAA	9 cut(s)	59, 650, 683, 879, 1095, 1231, 1304, 1719, 2424
TspGWI	ACGGA	3 cut(s)	1679, 1758, 2461
TspRI	CASTG	2 cut(s)	279, 838
Vha464I	CTTAAG	1 cut(s)	2294
VneI	GTGCAC	2 cut(s)	1551, 2321
VpaK11BI	GGWCC	1 cut(s)	2402
VspI	ATTAAT	1 cut(s)	1409
XagI	CCTNNNNNAGG	2 cut(s)	1107, 1560
XapI	RAATTY	3 cut(s)	1042, 1067, 1274
XceI	RCATGY	1 cut(s)	1934
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1459
XmiI	GTMKAC	1 cut(s)	2017
XmnI	GAANNNNTTC	1 cut(s)	849
XspI	CTAG	4 cut(s)	479, 1343, 2021, 2180
Zsp2I	ATGCAT	1 cut(s)	1833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.