Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0003931

Physical Location & Seq

Genomic Coordinates Reverse (-)

32510963 .. 32515601

Gene Structure 4639 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0187300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4050 bp

ATGGTGATCATTGAAGAAGCCTCTTCTTCTTCCACCCGTCCATGGAAATACCATGTCTTTTTGAGTTTTAGAGGTGAGGATACACGCTACAATTTCACAGACCATTTGTACAACGCTTTGCGTCGGGAGGGAATCATCACCTTTATGGATGATCAGCTCAGAAGAGGAGAAGAAATATCAACAGAACTTCTCCAAGCAATTGATCAGTCAAGGATTTCAATCATCGTCTTCTCTAAAAACTATGCATCCTCCAAGTGGTGCTTGGAAGAACTTGTCAAGATCCTCGACTGCAAGAAATCAAATCAACAAGAGGTTCGACCAGTTTTCTACAAAGTCAATCCCTCGGATATACGAAATCACAGAGGTAGCTTTGGGGAGGCACTTGCTAATCATGAATGCAAATTCAAAGATCACCCGGACAAGGTCCGGAAATGGAAGAATGCTCTTGAAGAAGCGGCAAATTTATCAGGGTGGCCTCTCTTGGAAGAGCATTGTTCTGAATCCAGTGTTATTCATGAAATTGTTGAAGAGATTTCAGAGCAAGTGATAAGCAGTACATATTTGGATGTGGCTAAGTACCCAGTAGGAATAAAACCTCGGGTGCAACAGATCCGTAACCTTTTAGGTGTCGGGGGAAGAGATGTTCGTATGGTAGGGATATGGGGGCCCGGTGGAATAGGCAAGACAACAATTGCCAAAGCTGTCTATAACTCAATTGCTCATAAATTTGATGGGAGTTGTTTTTTGGAAAATGTTAGAGAAAATTCAATGGGTGCCAAAGGTTTTGTCAAACTACAAAAGAAACTTCTTTGTGAGATTCTAAAGGGAACAAAATTGAAGGTGACCAATGTAGCAAGAGGAATCACTATGATTAAGGAAAGGTTGCAATACAAAAGTGTTCTCTTAGTTCTTGATGATGTGAATGGCATGGATCAAATAGATAACTTAGCTGGTCAATGTAGTTGGTTTGGAATGGGTAGTAGAATCATCATAACAACAAGGGATAAACAATTGCTGAGGCATCATAATGTTAATAATTTAATATACGAGGTCCAGAATTTGGATCATCATGAAGCTCTCGAGCTATTAAGTATGAATGCCTTTAAAAGAAGTCGACCTTTGGATGCTTATGCAGAACTCACAGAACATGCAATACGCTATGCTCAAGGCCTTCCGTTAGCTTTGACTGTTTTAGGTTCTTCTCTATGCAATGAAACTGCAGAGTTGTGGGAAGCTGTATTAGATGGTTTCAAAAGCAGCAAAATTCAAGATGTTTTAAAAATAAGTTACAACGGATTGGATCACATATTGAAGGAAGCTTTTCTTGACATTGCATGTTTCTTTAAAGGTGAAGGTAGAGCACACGTGATAGAAATATTAGAAGCTTGTGGTTGCAAACATGCTAAGTTTGTTATCAATGTGCTGGTAGAAAAGGCCCTCATTAGAATTACATACCATGATTCCATTTGGATGCATGACTTGGTAGAAAAAATGGGTAGAGATATAGTTCATGAATTGTCACCCAATGACCCTGGAAACCGAAGCAGGTTGTGGTTTCATGAAGATGTTTACCGTGTTCTAACAGAGAATATTGGAACAACCAATGTTATAGGCATCAAGGTGCAGCTGCCTGAAGATTCAGATGTGATATGTTTTTGTGGTACCACTTTCTCCAACATGCGAAATCTTAGACTTCTCATACACCGTGCTGGACGCTATTCTGGAGTTGTTGATAGTCTGCCAAATAGCTTAAGGGTAGTTGATTGGCCTGATTGTCCCCTACAATTTTTGCCATCCCATTCAATACCAAAACAACTTGCTATAATCAATATGCCTAGGAGTACTCGCATCACAGTATTAGGAGATGGATACAAGGATCCGATAAATCTCACATCAATAAATTTAAGTGGTTGTCAATACCTAACAAAAGTGTCAGACTTATCAAAAATCCCACACTTACGAAGTTTGAATCTAGCTGGCTGTGAAAATTTAGTTGAAGTGCATTCTTCTGTTGGATTCCTCGATAAACTTGACTGTTTGAATCTTTGGGACTGCCCTCGGCTTGAGACTTTTCCAACGGAAGTTAGTTGGAAATCCATGAGGAATCTTTACCTTGGCTGTTGTGAAAGGCTTGAGAATTTCATAAACATTGTGCACAAGATGGAGTCCATTAAACAATTGGATCTAGGTGGAAGTGGCATAACAGAACTGCACTCCTGGATTGGATGTCGCACTTCCTTGACCGAATTGAAGTTGTGTCAAACTCCGATCAAACAACTGCCTTCAACGATTGAAAATCTTACTTCTTTAACATATTTGGAGCTGTCTAAAACTCCCATCAGAGAATTGCCTTCATCGATTGGATGTCTCACTTCCTTGACGGAATTGAAGGCGAATGGAAGTCTCATCGAAGTACTGCCTTCATCAATTGGGAATCTCACTTCTTTAGAAAGATTGGACTTGTCTGAAACTCCCATCATAGAATTGCCTTCATCAATTGGGGATCTCACTTCCTTGGAGGAATTGGACGTGTCTGAAACTCTCATTGAAGAATTGCCTTCATCAATTGGGGATCTGACTTCTTTGAAGCGATTGAACCTGTCTAAAACTCCTATCAGAGAATTGCCTTCATCAATTGGGGATCTCATTGGGCTTCAATATTTAAATCTAAAAGGATGTGCAAACCATACAAATATGCCGCACAGTGTTTACGGAGGGCTCCAGCATCTGGAAAAACTAGACCTCTCTTGGTGCCCAAAACTGGTGACATTTCCAAGTAGGGGGAGCGCTTTGGTTTCATCAAGTGCAGAATCTCTTCCTTTGATGCTTCCATCTAATTCAAACAATGGGCATGATCATCATCCTGGTTCATTATTGTTTCCCCAGCTACAGTCGCTTTATTTTGAAGGATGCCAATTATCAGTGGTCTCTGATTTCCTAACGAATCTTGATTGTGTGTCCACATTACTGAGCCTTAATCTATCAGGAAGCAGTTTTGATAGTCTTCCGGCATGCATCAGCAAATTTCATCAGTTGTATGCACTTGATTTGAGTGGTTGCAAGAGGCTTCAAGACATTTCCGAACTTCCACCAAATATACGAGTTATAAACCTGGATGATTGCGTATCGTTGGAAAGATTTTCAAAATTGTCAAATATACTGGAACAGAAAGACACTCCGGGACGTCTTCACATTATGAATTTGTCTAATTGCAATAGACTGATGGATAATCTTGGCATGGACGTGGTGTCGAAGATGGCAAAAGCATTACCGTATCAGCTGGTGCATGCCGGCTTAGATCAGCATTTGAAGTGCTGGAACTTTATGCTTCCAAGCCTCCCGGAAATTGAAGTTCCAAAGTGGTTTGATAGGGGTGAGGTGGACACAAGTGTGCTTCCTGGTCATGGAACTCTTGGTATATGTGAAATTTTGATAGAAATCCCTAGGAATCTGAAGGCTGAGAGAATAGGATTGGTTGTCTGTGTTGTTTTTGAACTGATCCAAGATTTTTCTGGTGGTTATAATAGTTGCTCTGTGAGGGTTCTGATTGATAAAAAGTATTATGACATTAGTGTCAACTACATTGAATCAAAAGCGACAGAGTCATCTGCTCGTGATCATGTATGTGTGTGTCTGACATGTATTGATTTCAAGGAGCCACCTCTAGATCCTGTGGTTAGAGTGTTCTTTGATATGATTCCCCCCCGTGGCAAAGGGTTGCTCCTAAAAAGTTTCGGGGTCCACCTGGCTAATATGAAAGATGATCATGTAAGAGGTGAAGACTTGACACGAGAAGGATATAGGCAGAGCAATGCTAGCATTTCTGACCAGGTGGCTTTGGACACAGAAGGGGCAGCTGTAGCATCATTAGTATCAGATGGCAGCAATTCCGGTGAAGTACATGACGATCATCATGATGGGGAACAAGAACAACAACGAGAACAGGAACCTCATCTTCCAACAAAAAGATTTGAAGACCTGGCAAGACAAACAAATACAGGCAGAGCAATGTTACCTTTTTTGAGGTGCTTTGGAATTACCTGCTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003995 GO:0005488 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016042 GO:0016049 GO:0016054 GO:0016491 GO:0016627 GO:0019395 GO:0019752 GO:0021700 GO:0022622 GO:0030154 GO:0030258 GO:0032501 GO:0032502 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0040007 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0046395 GO:0048037 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050660 GO:0050662 GO:0055114 GO:0060560 GO:0071695 GO:0071704 GO:0072329 GO:0080147 GO:0090558 GO:0090627 GO:0097159 GO:0099402 GO:1901265 GO:1901363 GO:1901575 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko00071 ko00280 ko00410 ko00640 ko01100 ko01110 ko01130 ko01200 ko01212 ko03320 map00071 map00280 map00410 map00640 map01100 map01110 map01130 map01200 map01212 map03320

Pfam Domains

Protein Families

APH Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

1349

Amino Acids

151.05

Weight (kDa)

5.9

Isoelectric Point (pI)

42.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	16 - 183	3.5e-52	TIR domain
TIR_2	PF13676	19 - 113	3.9e-16	TIR domain
NB-ARC	PF00931	201 - 370	2.5e-19	NB-ARC domain
WHD_ROQ1	PF23282	438 - 507	1e-16	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	747 - 821	2.2e-14	Leucine-rich repeat region
LRR_14	PF23598	819 - 865	2.2e-09	Leucine-rich repeat region
LRR_13	PF23286	836 - 1058	2.7e-13	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	856 - 920	8.8e-08	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0003931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06810
fragaria_vesca	FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700
malus_domestica	MD13G1164300.v1.1 MD16G1164100.v1.1
prunus_persica	Prupe.1G131800_v2.0.a1 Prupe.1G131800_v2.0.a1 Prupe.1G131800_v2.0.a1
pyrus_communis	pycom13g14030 pycom16g13880 pycom16g13890
rosa_chinensis	RchiOBHm_Chr3g0480631 RchiOBHm_Chr4g0423401 RchiOBHm_Chr6g0311831
rosa_laevigata	RLG00000007561
rosa_multiflora	Rmu_sc0002752.1_g000004 Rmu_ssc0000174.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365600
rosa_rugosa	Rorug04G0187300
rosa_samantha	Rh1BG304700 Rh3AG236600 Rh3DG262000 Rh4AG246200 Rh4AG246300 Rh4BG251700 Rh4CG260200 Rh4DG247100
rosa_wichuraiana	Rw4G021160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	3107, 3522
AasI	GACNNNNNNGTC	1 cut(s)	3572
AatII	GACGTC	1 cut(s)	3187
Acc36I	ACCTGC	1 cut(s)	1536
Acc65I	GGTACC	1 cut(s)	1661
AccB1I	GGYRCC	3 cut(s)	773, 1661, 2751
AccB7I	CCANNNNNTGG	2 cut(s)	1060, 2728
AccI	GTMKAC	1 cut(s)	1114
AccIII	TCCGGA	1 cut(s)	426
AciI	CCGC	2 cut(s)	455, 2699
AcuI	CTGAAG	2 cut(s)	1653, 3473
AcvI	CACGTG	1 cut(s)	1366
AcyI	GRCGYC	1 cut(s)	3184
AfaI	GTAC	7 cut(s)	110, 556, 578, 1663, 1843, 2415, 3900
AfeI	AGCGCT	1 cut(s)	2788
AfiI	CCNNNNNNNGG	9 cut(s)	42, 255, 421, 1060, 2728, 2761, 2780, 3404, 3707
AflII	CTTAAG	1 cut(s)	1750
AflIII	ACRYGT	3 cut(s)	1363, 2529, 3638
AhdI	GACNNNNNGTC	1 cut(s)	3247
AjiI	CACGTC	2 cut(s)	2530, 3244
AjnI	CCWGG	8 cut(s)	1531, 2216, 2863, 3111, 3397, 3744, 3828, 3978
AleI	CACNNNNGTG	1 cut(s)	3389
AloI	GAACNNNNNNTCC	2 cut(s)	627, 659
Alw21I	GWGCWC	2 cut(s)	1363, 2157
Alw26I	GTCTC	4 cut(s)	2060, 2372, 2408, 2932
Alw44I	GTGCAC	1 cut(s)	2153
AlwNI	CAGNNNCTG	1 cut(s)	2728
Ama87I	CYCGRG	2 cut(s)	597, 1079
Aor13HI	TCCGGA	1 cut(s)	426
Aor51HI	AGCGCT	1 cut(s)	2788
AoxI	GGCC	5 cut(s)	473, 665, 1168, 1434, 1766
ApaI	GGGCCC	1 cut(s)	669
ApaLI	GTGCAC	1 cut(s)	2153
ApeKI	GCWGC	5 cut(s)	1257, 1624, 1627, 3854, 3882
ArsI	GACNNNNNNTTYG	2 cut(s)	771, 803
Asp700I	GAANNNNTTC	2 cut(s)	1320, 2814
Asp718I	GGTACC	1 cut(s)	1661
AspA2I	CCTAGG	2 cut(s)	1835, 3443
AspLEI	GCGC	1 cut(s)	2789
AspS9I	GGNCC	6 cut(s)	424, 665, 666, 1051, 1435, 3739
AsuC2I	CCSGG	4 cut(s)	416, 669, 3180, 3341
AsuNHI	GCTAGC	1 cut(s)	3815
AvaI	CYCGRG	2 cut(s)	597, 1079
AvaII	GGWCC	3 cut(s)	424, 1051, 3739
AvrII	CCTAGG	2 cut(s)	1835, 3443
BaeGI	GKGCMC	3 cut(s)	669, 2157, 2756
BamHI	GGATCC	1 cut(s)	1876
BanI	GGYRCC	3 cut(s)	773, 1661, 2751
BanII	GRGCYC	2 cut(s)	669, 2721
BauI	CACGAG	2 cut(s)	3612, 3789
BbrPI	CACGTG	1 cut(s)	1366
BbsI	GAAGAC	5 cut(s)	220, 2996, 3179, 3786, 3981
Bbv12I	GWGCWC	2 cut(s)	1363, 2157
BbvCI	CCTCAGC	1 cut(s)	1016
BbvI	GCAGC	5 cut(s)	1269, 1614, 1636, 3866, 3894
BciT130I	CCWGG	8 cut(s)	1533, 2218, 2865, 3113, 3399, 3746, 3830, 3980
BciVI	GTATCC	2 cut(s)	73, 1862
BclI	TGATCA	6 cut(s)	6, 151, 202, 2854, 3616, 3763
BcnI	CCSGG	4 cut(s)	416, 669, 3180, 3341
BcoDI	GTCTC	4 cut(s)	2060, 2372, 2408, 2932
BfaI	CTAG	7 cut(s)	1836, 1973, 2186, 2738, 3444, 3665, 3816
BfmI	CTRYAG	3 cut(s)	1218, 2888, 3858
BfoI	RGCGCY	1 cut(s)	2790
BfrI	CTTAAG	1 cut(s)	1750
BfuAI	ACCTGC	1 cut(s)	1536
BfuI	GTATCC	2 cut(s)	73, 1862
BisI	GCNGC	7 cut(s)	456, 1258, 1625, 1628, 2699, 3855, 3883
BlnI	CCTAGG	2 cut(s)	1835, 3443
BlsI	GCNGC	7 cut(s)	457, 1259, 1626, 1629, 2700, 3856, 3884
BmcAI	AGTACT	2 cut(s)	1843, 2415
Bme18I	GGWCC	3 cut(s)	424, 1051, 3739
BmeRI	GACNNNNNGTC	1 cut(s)	3247
BmeT110I	CYCGRG	2 cut(s)	597, 1079
BmgBI	CACGTC	2 cut(s)	2530, 3244
BmgT120I	GGNCC	6 cut(s)	424, 665, 666, 1051, 1435, 3739
BmrI	ACTGGG	1 cut(s)	575
BmtI	GCTAGC	1 cut(s)	3819
BmuI	ACTGGG	1 cut(s)	575
BpiI	GAAGAC	5 cut(s)	220, 2996, 3179, 3786, 3981
BpmI	CTGGAG	2 cut(s)	1743, 2705
Bpu10I	CCTNAGC	1 cut(s)	1016
BpuEI	CTTGAG	3 cut(s)	1149, 2084, 2153
BpuMI	CCSGG	4 cut(s)	416, 669, 3180, 3341
Bsa29I	ATCGAT	1 cut(s)	2357
BsaAI	YACGTR	1 cut(s)	1366
BsaBI	GATNNNNATC	3 cut(s)	218, 2859, 3437
BsaHI	GRCGYC	1 cut(s)	3184
BsaI	GGTCTC	1 cut(s)	2932
BsaWI	WCCGGW	2 cut(s)	426, 3890
Bsc4I	CCNNNNNNNGG	9 cut(s)	42, 255, 421, 1060, 2728, 2761, 2780, 3404, 3707
Bse118I	RCCGGY	1 cut(s)	3290
Bse1I	ACTGG	5 cut(s)	320, 504, 581, 2766, 3165
Bse3DI	GCAATG	4 cut(s)	1216, 1329, 3817, 4014
Bse8I	GATNNNNATC	3 cut(s)	218, 2859, 3437
BseAI	TCCGGA	1 cut(s)	426
BseBI	CCWGG	8 cut(s)	1533, 2218, 2865, 3113, 3399, 3746, 3830, 3980
BseCI	ATCGAT	1 cut(s)	2357
BseJI	GATNNNNATC	3 cut(s)	218, 2859, 3437
BseLI	CCNNNNNNNGG	9 cut(s)	42, 255, 421, 1060, 2728, 2761, 2780, 3404, 3707
BseMI	GCAATG	4 cut(s)	1216, 1329, 3817, 4014
BseMII	CTCAG	4 cut(s)	172, 1007, 2960, 3450
BseNI	ACTGG	5 cut(s)	320, 504, 581, 2766, 3165
BseRI	GAGGAG	1 cut(s)	180
BseSI	GKGCMC	3 cut(s)	669, 2157, 2756
BseXI	GCAGC	5 cut(s)	1269, 1614, 1636, 3866, 3894
BseYI	CCCAGC	1 cut(s)	2883
BsgI	GTGCAG	3 cut(s)	1643, 2195, 2826
BshFI	GGCC	5 cut(s)	475, 667, 1170, 1436, 1768
BshNI	GGYRCC	3 cut(s)	773, 1661, 2751
BshVI	ATCGAT	1 cut(s)	2357
BsiHKAI	GWGCWC	2 cut(s)	1363, 2157
BsiHKCI	CYCGRG	2 cut(s)	597, 1079
BsiSI	CCGG	8 cut(s)	416, 427, 669, 3008, 3179, 3291, 3341, 3891
BslFI	GGGAC	3 cut(s)	1761, 2063, 3195
BslI	CCNNNNNNNGG	9 cut(s)	42, 255, 421, 1060, 2728, 2761, 2780, 3404, 3707
BsmAI	GTCTC	4 cut(s)	2060, 2372, 2408, 2932
BsmFI	GGGAC	3 cut(s)	1761, 2063, 3195
BsmI	GAATGC	4 cut(s)	401, 445, 1102, 2002
BsnI	GGCC	5 cut(s)	475, 667, 1170, 1436, 1768
Bso31I	GGTCTC	1 cut(s)	2932
BsoBI	CYCGRG	2 cut(s)	597, 1079
Bsp120I	GGGCCC	1 cut(s)	665
Bsp1286I	GDGCHC	5 cut(s)	669, 1363, 2157, 2721, 2756
Bsp13I	TCCGGA	1 cut(s)	426
Bsp1407I	TGTACA	1 cut(s)	108
Bsp19I	CCATGG	1 cut(s)	41
BspACI	CCGC	2 cut(s)	455, 2699
BspANI	GGCC	5 cut(s)	475, 667, 1170, 1436, 1768
BspCNI	CTCAG	4 cut(s)	171, 1008, 2961, 3451
BspDI	ATCGAT	1 cut(s)	2357
BspEI	TCCGGA	1 cut(s)	426
BspHI	TCATGA	6 cut(s)	391, 514, 1069, 1510, 1558, 3913
BspMAI	CTGCAG	1 cut(s)	1222
BspMI	ACCTGC	1 cut(s)	1536
BspOI	GCTAGC	1 cut(s)	3819
BspQI	GCTCTTC	1 cut(s)	480
BspT107I	GGYRCC	3 cut(s)	773, 1661, 2751
BspTI	CTTAAG	1 cut(s)	1750
BspTNI	GGTCTC	1 cut(s)	2932
BsrDI	GCAATG	4 cut(s)	1216, 1329, 3817, 4014
BsrFI	RCCGGY	1 cut(s)	3290
BsrGI	TGTACA	1 cut(s)	108
BsrI	ACTGG	5 cut(s)	320, 504, 581, 2766, 3165
BssAI	RCCGGY	1 cut(s)	3290
BssNI	GRCGYC	1 cut(s)	3184
BssSI	CACGAG	2 cut(s)	3612, 3789
BssT1I	CCWWGG	5 cut(s)	41, 1835, 2113, 2514, 3443
Bst2BI	CACGAG	2 cut(s)	3612, 3789
Bst2UI	CCWGG	8 cut(s)	1533, 2218, 2865, 3113, 3399, 3746, 3830, 3980
Bst4CI	ACNGT	8 cut(s)	1189, 1574, 1706, 1855, 2036, 2705, 2892, 3273
Bst6I	CTCTTC	6 cut(s)	28, 157, 480, 522, 631, 2820
BstACI	GRCGYC	1 cut(s)	3184
BstAFI	CTTAAG	1 cut(s)	1750
BstAUI	TGTACA	1 cut(s)	108
BstBAI	YACGTR	1 cut(s)	1366
BstC8I	GCNNGC	5 cut(s)	1978, 3013, 3288, 3292, 3817
BstDSI	CCRYGG	2 cut(s)	41, 3706
BstEII	GGTNACC	1 cut(s)	841
BstH2I	RGCGCY	1 cut(s)	2790
BstHHI	GCGC	1 cut(s)	2789
BstMAI	GTCTC	4 cut(s)	2060, 2372, 2408, 2932
BstMWI	GCNNNNNNNGC	3 cut(s)	2893, 3816, 3860
BstNI	CCWGG	8 cut(s)	1533, 2218, 2865, 3113, 3399, 3746, 3830, 3980
BstNSI	RCATGY	7 cut(s)	1151, 1338, 1403, 1681, 3015, 3290, 3642
BstPI	GGTNACC	1 cut(s)	841
BstSFI	CTRYAG	3 cut(s)	1218, 2888, 3858
BstSLI	GKGCMC	3 cut(s)	669, 2157, 2756
BstV1I	GCAGC	5 cut(s)	1269, 1614, 1636, 3866, 3894
BstV2I	GAAGAC	5 cut(s)	220, 2996, 3179, 3786, 3981
BstX2I	RGATCY	8 cut(s)	279, 609, 1876, 2182, 2503, 2572, 2641, 3667
BstYI	RGATCY	8 cut(s)	279, 609, 1876, 2182, 2503, 2572, 2641, 3667
Bsu15I	ATCGAT	1 cut(s)	2357
BsuI	GTATCC	2 cut(s)	73, 1862
BsuRI	GGCC	5 cut(s)	475, 667, 1170, 1436, 1768
BsuTUI	ATCGAT	1 cut(s)	2357
BtgI	CCRYGG	2 cut(s)	41, 3706
BtrI	CACGTC	2 cut(s)	2530, 3244
BtsIMutI	CAGTG	3 cut(s)	511, 2710, 2928
BveI	ACCTGC	1 cut(s)	1536
Cac8I	GCNNGC	5 cut(s)	1978, 3013, 3288, 3292, 3817
CaiI	CAGNNNCTG	1 cut(s)	2728
CciI	TCATGA	6 cut(s)	391, 514, 1069, 1510, 1558, 3913
CfoI	GCGC	1 cut(s)	2789
Cfr10I	RCCGGY	1 cut(s)	3290
Cfr13I	GGNCC	6 cut(s)	424, 665, 666, 1051, 1435, 3739
ClaI	ATCGAT	1 cut(s)	2357
CseI	GACGC	2 cut(s)	110, 1722
CsiI	ACCWGGT	1 cut(s)	3828
Csp6I	GTAC	7 cut(s)	109, 555, 577, 1662, 1842, 2414, 3899
CviQI	GTAC	7 cut(s)	109, 555, 577, 1662, 1842, 2414, 3899
DraI	TTTAAA	4 cut(s)	1105, 1278, 1345, 2664
DrdI	GACNNNNNNGTC	1 cut(s)	3572
DriI	GACNNNNNGTC	1 cut(s)	3247
DseDI	GACNNNNNNGTC	1 cut(s)	3572
Eam1104I	CTCTTC	6 cut(s)	28, 157, 480, 522, 631, 2820
Eam1105I	GACNNNNNGTC	1 cut(s)	3247
EarI	CTCTTC	6 cut(s)	28, 157, 480, 522, 631, 2820
Eco130I	CCWWGG	5 cut(s)	41, 1835, 2113, 2514, 3443
Eco147I	AGGCCT	1 cut(s)	1170
Eco24I	GRGCYC	2 cut(s)	669, 2721
Eco31I	GGTCTC	1 cut(s)	2932
Eco47I	GGWCC	3 cut(s)	424, 1051, 3739
Eco47III	AGCGCT	1 cut(s)	2788
Eco57I	CTGAAG	2 cut(s)	1653, 3473
Eco72I	CACGTG	1 cut(s)	1366
Eco88I	CYCGRG	2 cut(s)	597, 1079
Eco91I	GGTNACC	1 cut(s)	841
EcoO109I	RGGNCCY	2 cut(s)	665, 1435
EcoO65I	GGTNACC	1 cut(s)	841
EcoRII	CCWGG	8 cut(s)	1531, 2216, 2863, 3111, 3397, 3744, 3828, 3978
EcoT14I	CCWWGG	5 cut(s)	41, 1835, 2113, 2514, 3443
EcoT22I	ATGCAT	3 cut(s)	247, 1476, 3017
EcoT38I	GRGCYC	2 cut(s)	669, 2721
ErhI	CCWWGG	5 cut(s)	41, 1835, 2113, 2514, 3443
FalI	AAGNNNNNCTT	6 cut(s)	245, 277, 1102, 1134, 1308, 1340
FaqI	GGGAC	3 cut(s)	1761, 2063, 3195
FbaI	TGATCA	6 cut(s)	6, 151, 202, 2854, 3616, 3763
FblI	GTMKAC	1 cut(s)	1114
Fnu4HI	GCNGC	7 cut(s)	456, 1258, 1625, 1628, 2699, 3855, 3883
FriOI	GRGCYC	2 cut(s)	669, 2721
Fsp4HI	GCNGC	7 cut(s)	456, 1258, 1625, 1628, 2699, 3855, 3883
FspBI	CTAG	7 cut(s)	1836, 1973, 2186, 2738, 3444, 3665, 3816
GlaI	GCGC	1 cut(s)	2788
GluI	GCNGC	7 cut(s)	456, 1258, 1625, 1628, 2699, 3855, 3883
GsaI	CCCAGC	1 cut(s)	2887
GsuI	CTGGAG	2 cut(s)	1743, 2705
HaeII	RGCGCY	1 cut(s)	2790
HaeIII	GGCC	5 cut(s)	475, 667, 1170, 1436, 1768
HapII	CCGG	8 cut(s)	416, 427, 669, 3008, 3179, 3291, 3341, 3891
HgaI	GACGC	2 cut(s)	110, 1722
HhaI	GCGC	1 cut(s)	2789
Hin1I	GRCGYC	1 cut(s)	3184
Hin6I	GCGC	1 cut(s)	2787
HinP1I	GCGC	1 cut(s)	2787
HincII	GTYRAC	2 cut(s)	1115, 3577
HindII	GTYRAC	2 cut(s)	1115, 3577
HindIII	AAGCTT	2 cut(s)	1317, 1383
HpaII	CCGG	8 cut(s)	416, 427, 669, 3008, 3179, 3291, 3341, 3891
Hpy166II	GTNNAC	8 cut(s)	1115, 1570, 2155, 2710, 2961, 3382, 3577, 3742
Hpy8I	GTNNAC	8 cut(s)	1115, 1570, 2155, 2710, 2961, 3382, 3577, 3742
Hpy99I	CGWCG	1 cut(s)	126
HpyCH4III	ACNGT	8 cut(s)	1189, 1574, 1706, 1855, 2036, 2705, 2892, 3273
HpyCH4IV	ACGT	4 cut(s)	1365, 2529, 3184, 3243
HpyF10VI	GCNNNNNNNGC	3 cut(s)	2893, 3816, 3860
HpySE526I	ACGT	4 cut(s)	1365, 2529, 3184, 3243
Hsp92I	GRCGYC	1 cut(s)	3184
HspAI	GCGC	1 cut(s)	2787
Kpn2I	TCCGGA	1 cut(s)	426
KpnI	GGTACC	1 cut(s)	1665
KroI	GCCGGC	1 cut(s)	3290
KroNI	GCCGGC	1 cut(s)	3292
Ksp22I	TGATCA	6 cut(s)	6, 151, 202, 2854, 3616, 3763
LguI	GCTCTTC	1 cut(s)	480
LmnI	GCTCC	5 cut(s)	2320, 2724, 2784, 3655, 3726
Lsp1109I	GCAGC	5 cut(s)	1269, 1614, 1636, 3866, 3894
MabI	ACCWGGT	1 cut(s)	3828
MaeI	CTAG	7 cut(s)	1836, 1973, 2186, 2738, 3444, 3665, 3816
MaeII	ACGT	4 cut(s)	1365, 2529, 3184, 3243
MaeIII	GTNAC	6 cut(s)	614, 841, 1286, 1518, 2764, 4011
MfeI	CAATTG	9 cut(s)	198, 690, 714, 1010, 2177, 2427, 2496, 2565, 2634
MflI	RGATCY	8 cut(s)	279, 609, 1876, 2182, 2503, 2572, 2641, 3667
MhlI	GDGCHC	5 cut(s)	669, 1363, 2157, 2721, 2756
MlyI	GAGTC	2 cut(s)	2174, 3611
MmeI	TCCRAC	6 cut(s)	1698, 1993, 2069, 2099, 3111, 3983
Mph1103I	ATGCAT	3 cut(s)	247, 1476, 3017
MroI	TCCGGA	1 cut(s)	426
MroNI	GCCGGC	1 cut(s)	3290
MroXI	GAANNNNTTC	2 cut(s)	1320, 2814
MslI	CAYNNNNRTG	9 cut(s)	143, 1026, 1469, 1563, 1619, 3242, 3389, 3624, 3915
MspA1I	CMGCKG	3 cut(s)	1627, 3280, 3857
MspCI	CTTAAG	1 cut(s)	1750
MspI	CCGG	8 cut(s)	416, 427, 669, 3008, 3179, 3291, 3341, 3891
MunI	CAATTG	9 cut(s)	198, 690, 714, 1010, 2177, 2427, 2496, 2565, 2634
Mva1269I	GAATGC	4 cut(s)	401, 445, 1102, 2002
MvaI	CCWGG	8 cut(s)	1533, 2218, 2865, 3113, 3399, 3746, 3830, 3980
MwoI	GCNNNNNNNGC	3 cut(s)	2893, 3816, 3860
NaeI	GCCGGC	1 cut(s)	3292
NciI	CCSGG	4 cut(s)	416, 669, 3180, 3341
NcoI	CCATGG	1 cut(s)	41
NgoMIV	GCCGGC	1 cut(s)	3290
NheI	GCTAGC	1 cut(s)	3815
NmeAIII	GCCGAG	1 cut(s)	2038
NmuCI	GTSAC	3 cut(s)	841, 1518, 2764
NsiI	ATGCAT	3 cut(s)	247, 1476, 3017
NspI	RCATGY	7 cut(s)	1151, 1338, 1403, 1681, 3015, 3290, 3642
OliI	CACNNNNGTG	1 cut(s)	3389
PaeI	GCATGC	2 cut(s)	3015, 3290
PaeR7I	CTCGAG	1 cut(s)	1079
PagI	TCATGA	6 cut(s)	391, 514, 1069, 1510, 1558, 3913
PceI	AGGCCT	1 cut(s)	1170
PciI	ACATGT	1 cut(s)	3638
PciSI	GCTCTTC	1 cut(s)	480
PctI	GAATGC	4 cut(s)	401, 445, 1102, 2002
PdiI	GCCGGC	1 cut(s)	3292
PdmI	GAANNNNTTC	2 cut(s)	1320, 2814
PflFI	GACNNNGTC	2 cut(s)	422, 3601
PflMI	CCANNNNNTGG	2 cut(s)	1060, 2728
PfoI	TCCNGGA	3 cut(s)	2216, 3178, 3339
PkrI	GCNGC	7 cut(s)	457, 1259, 1626, 1629, 2700, 3856, 3884
PleI	GAGTC	2 cut(s)	2173, 3610
PmaCI	CACGTG	1 cut(s)	1366
PmlI	CACGTG	1 cut(s)	1366
PpsI	GAGTC	2 cut(s)	2173, 3610
Ppu21I	YACGTR	1 cut(s)	1366
PscI	ACATGT	1 cut(s)	3638
PsiI	TTATAA	2 cut(s)	3107, 3522
Psp6I	CCWGG	8 cut(s)	1531, 2216, 2863, 3111, 3397, 3744, 3828, 3978
PspCI	CACGTG	1 cut(s)	1366
PspEI	GGTNACC	1 cut(s)	841
PspFI	CCCAGC	1 cut(s)	2883
PspGI	CCWGG	8 cut(s)	1531, 2216, 2863, 3111, 3397, 3744, 3828, 3978
PspOMI	GGGCCC	1 cut(s)	665
PspPI	GGNCC	6 cut(s)	424, 665, 666, 1051, 1435, 3739
PsrI	GAACNNNNNNTAC	2 cut(s)	1137, 1169
PstI	CTGCAG	1 cut(s)	1222
PstNI	CAGNNNCTG	1 cut(s)	2728
PsuI	RGATCY	8 cut(s)	279, 609, 1876, 2182, 2503, 2572, 2641, 3667
PsyI	GACNNNGTC	2 cut(s)	422, 3601
PvuII	CAGCTG	3 cut(s)	1627, 3280, 3857
RsaI	GTAC	7 cut(s)	110, 556, 578, 1663, 1843, 2415, 3900
RsaNI	GTAC	7 cut(s)	109, 555, 577, 1662, 1842, 2414, 3899
RseI	CAYNNNNRTG	9 cut(s)	143, 1026, 1469, 1563, 1619, 3242, 3389, 3624, 3915
SalI	GTCGAC	1 cut(s)	1113
SapI	GCTCTTC	1 cut(s)	480
SatI	GCNGC	7 cut(s)	456, 1258, 1625, 1628, 2699, 3855, 3883
Sau96I	GGNCC	6 cut(s)	424, 665, 666, 1051, 1435, 3739
ScaI	AGTACT	2 cut(s)	1843, 2415
SchI	GAGTC	2 cut(s)	2174, 3611
SduI	GDGCHC	5 cut(s)	669, 1363, 2157, 2721, 2756
SexAI	ACCWGGT	1 cut(s)	3828
SfcI	CTRYAG	3 cut(s)	1218, 2888, 3858
Sfr274I	CTCGAG	1 cut(s)	1079
SinI	GGWCC	3 cut(s)	424, 1051, 3739
SlaI	CTCGAG	1 cut(s)	1079
SmiI	ATTTAAAT	1 cut(s)	2664
SmiMI	CAYNNNNRTG	9 cut(s)	143, 1026, 1469, 1563, 1619, 3242, 3389, 3624, 3915
SmlI	CTYRAG	5 cut(s)	1079, 1164, 1750, 2063, 2132
SmoI	CTYRAG	5 cut(s)	1079, 1164, 1750, 2063, 2132
SphI	GCATGC	2 cut(s)	3015, 3290
SseBI	AGGCCT	1 cut(s)	1170
SsiI	CCGC	2 cut(s)	455, 2699
SspI	AATATT	3 cut(s)	1377, 1591, 2660
SspMI	CTAG	7 cut(s)	1836, 1973, 2186, 2738, 3444, 3665, 3816
StuI	AGGCCT	1 cut(s)	1170
StyI	CCWWGG	5 cut(s)	41, 1835, 2113, 2514, 3443
SwaI	ATTTAAAT	1 cut(s)	2664
TaaI	ACNGT	8 cut(s)	1189, 1574, 1706, 1855, 2036, 2705, 2892, 3273
TaiI	ACGT	4 cut(s)	1368, 2532, 3187, 3246
TaqI	TCGA	8 cut(s)	285, 316, 1080, 1114, 2022, 2357, 2409, 3251
TaqII	GACCGA	1 cut(s)	2258
TatI	WGTACW	5 cut(s)	108, 554, 1841, 2413, 3898
TauI	GCSGC	2 cut(s)	458, 2701
TscAI	CASTG	3 cut(s)	511, 2710, 2928
TseFI	GTSAC	3 cut(s)	841, 1518, 2764
TseI	GCWGC	5 cut(s)	1257, 1624, 1627, 3854, 3882
Tsp45I	GTSAC	3 cut(s)	841, 1518, 2764
TspGWI	ACGGA	6 cut(s)	602, 1164, 1308, 2093, 2396, 2727
TspRI	CASTG	3 cut(s)	511, 2710, 2928
Tth111I	GACNNNGTC	2 cut(s)	422, 3601
Van91I	CCANNNNNTGG	2 cut(s)	1060, 2728
Vha464I	CTTAAG	1 cut(s)	1750
VneI	GTGCAC	1 cut(s)	2153
VpaK11BI	GGWCC	3 cut(s)	424, 1051, 3739
XbaI	TCTAGA	1 cut(s)	3664
XceI	RCATGY	7 cut(s)	1151, 1338, 1403, 1681, 3015, 3290, 3642
XcmI	CCANNNNNNNNNTGG	3 cut(s)	259, 2176, 3509
XhoI	CTCGAG	1 cut(s)	1079
XmaJI	CCTAGG	2 cut(s)	1835, 3443
XmiI	GTMKAC	1 cut(s)	1114
XmnI	GAANNNNTTC	2 cut(s)	1320, 2814
XspI	CTAG	7 cut(s)	1836, 1973, 2186, 2738, 3444, 3665, 3816
ZraI	GACGTC	1 cut(s)	3185
ZrmI	AGTACT	2 cut(s)	1843, 2415
Zsp2I	ATGCAT	3 cut(s)	247, 1476, 3017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.