Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003931

Physical Location & Seq

Genomic Coordinates Reverse (-)

45671641 .. 45677961

Gene Structure 6321 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G021160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGGCTAACGAGCTCGACCTCGACCTCGACGCCTTGCTCCGCTACGCCGCCGCTAACGTCTCCGGCTTTCCTCCCTCGCCGTCCAATTTCACCGTCTCTAAGTTCGGGCATGGCCAATCGAATCCGACTTATCTGATGCAAGTAGGATCGGGAGCGTCAGTGAAACGCTACGTTTTGAGAAAGAAGCCGCCGGGGAAGTTGCTTCACTCTGCTCATGCTGTGGAGAGAGAATTCCAGGTTCTTCAAGCATTGGGTAATCATACTGTAGTTCCGGTTCCGAAAGTTTTCTGTTTGTGTACTGATCCAAGCGTGATTGGAACCGCTTTTTACATCATGGAGTTTTTGGAAGGGCGCATTTTTGTCGACCCCAGGCTACCGGGTGTAGAGCCTGCGAGCAGGAGGGCTATTTACCAAGCGACTGCAAAAGCTTTAGCATCGCTGCATTCTGCTGACATTGATGCCATTGGACTAGGAAACTATGGGCGTCGTGAAAACTATTGTAAACGGCAGGTAGAGAGGTGGGCAAAACAATATATTGCCTCCACTGGTGAAGGCAAGCCTGAGAGAAATCCGAAGATGTTTGAGCTGATTGATTGGTTACAGCAGCATATTCCTCTTGAAGATTCTTCAGGAGGTGCAACCGGCTTAGTGCATGGGGACTTTCGCCTCGATAATCTTGTGTTTCATCCTATTGAGGATCGAGTGGTTGGCATTCTTGACTGGGAATTGTCTACTCTTGGAAACCAAATGTGTGATGTTGCTTACTGTTCTTTGCCTTACATCACAGACTTGGGGGTTGACAAAGATCAACTTGGTAAAGGTATGGAACATACTGGGCTTCCAGAAGGGATTCCTTCTCAGGCAGAATATGTGGCAGAATACTGCTCTTCATCTGGAAAGCCATGGCCTTTTGCTCAGTGGAAGTTTTATATAGCATTTTCTTTATTCCGTGGCGCATCAATTTATGCGGGCATCTATAGTAGATGGACCATGGGTAATGCTTCAGGAGGTGAGAGTGCCCAGCATGCAGGTGACAAAGCTAATTTTCTTATAGACTATGCATGGGCATTTGTTAGACGAGAATCTGTGCTTCCTGAGCATCCTCCATCAGGGTCTTTTGTTGCTCAAAACTGCTTGAAAAGATTGGGACAAGAGAGTGAAGATCAAGGATTTTCAGAAGGGGGAGGAAAGTTTGTCCCCAGTAAAAGGATTTTAGAATTGAGAAAAAGGTTGGTCAAGTTCATGGAAGATCACATATATCCCATGGAAAAAGAATTCTACAAACTTGCTCAGTCTACCTCACGTTGGACAGTGCATCCAGAGGAGGAGAAGTTAAAGGAGCTCGCAAAGAAAGAAGGCCTATGGAACTTATTTATACCTTTTGATAGTGCTGCCAGGGCAAAAAAATTAATTTTTGATGGGACCAATCAACTTCAGTCAGATGATACCTACAATCAGTTATTGGGCGCTGGTCTTTCAAACCTTGAATACGGATATCTTTGTGAGATCATGGGTCGTTCTATTTGGGCTCCACAAGTGTTCAACTGTGGCGCACCTGATACAGGAAATATGGAGGTCTTGTTGCGTTATGGGAACAAAGAACAACTACTGGAATGGCTTATTCCTTTACTTGAGGGGAGAATCCGGTCAGGATTTGCAATGACAGAACCCCAAGTTGCATCCTCTGATGCAACTAACATCGAGTGTTCTATTAGTAGACAAGGAGATTCATATATTATCAATGGAAAAAAATGGTGGACAAGTGGGGCTATGGATCCAAGATGTAGACTTCTTATAGTCATGGGAAAAACCGACTTCAATGCTGCTATGCATAAACAACAGTCTATGATCTTAGTGGATATCCGGACTCCAGGTGTACATATAAAGAGGGCACTTACAGTTTTTGGCTTTGATGATGCACCTCATGGGCATGCAGAAGTTTTATTTGACAATGTACGTGTCCCAGCAAAGAATATTCTACTGGGAGAAGGACGTGGGTTTGAGATTGCACAGGGTAGGTTAGGCCCAGGAAGGTTACACCATTGCATGAGACTGATAGGAGCTGCTGAGCGAGGCATGGAGATAATGGCTGAAAGGGCTCTTAGTAGAAGAGTTTTTGGGAAGTTGATTGCTGAACAAGGCTCTTTTCGTTCTGATATAGCAAAGTGTCGAATAGAGCTAGAGAAAACCAGATTGTTGGTTCTGGAGGCAGCCGACCAGCTTGATAGACTTGGAAACAAAAAGGCCCGCGGAACTCTAGCAATGGCAAAGGTAGCAACCCCAAACATGGCTCTGATGGTGCTGGACATGGCAATGCAAGTCCATGGCGGCGCTGGCTTGTCTTCTGACACTTGTCTGGCTCATCTTTGGGCCACAGCAAGAACATTGAGAATAGCAGATGGTCCGGATGAAGTTCACTTGGGTACCATTGCCAAGTTAGAACTACAGAGAGCTAAAGCTAAACTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003995 GO:0005488 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016042 GO:0016049 GO:0016054 GO:0016491 GO:0016627 GO:0019395 GO:0019752 GO:0021700 GO:0022622 GO:0030154 GO:0030258 GO:0032501 GO:0032502 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0040007 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0046395 GO:0048037 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050660 GO:0050662 GO:0055114 GO:0060560 GO:0071695 GO:0071704 GO:0072329 GO:0080147 GO:0090558 GO:0090627 GO:0097159 GO:0099402 GO:1901265 GO:1901363 GO:1901575 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko00071 ko00280 ko00410 ko00640 ko01100 ko01110 ko01130 ko01200 ko01212 ko03320 map00071 map00280 map00410 map00640 map01100 map01110 map01130 map01200 map01212 map03320

Pfam Domains

Protein Families

APH Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

822

Amino Acids

91.2

Weight (kDa)

7.86

Isoelectric Point (pI)

41.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APH	PF01636	32 - 257	1.9e-48	Phosphotransferase enzyme family
Acyl-CoA_dh_M	PF02770	551 - 652	6.7e-18	Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1	PF00441	664 - 812	3.7e-35	Acyl-CoA dehydrogenase, C-terminal domain
Acyl-CoA_dh_2	PF08028	680 - 790	3.2e-06	Acyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06810
fragaria_vesca	FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700
malus_domestica	MD13G1164300.v1.1 MD16G1164100.v1.1
prunus_persica	Prupe.1G131800_v2.0.a1 Prupe.1G131800_v2.0.a1 Prupe.1G131800_v2.0.a1
pyrus_communis	pycom13g14030 pycom16g13880 pycom16g13890
rosa_chinensis	RchiOBHm_Chr3g0480631 RchiOBHm_Chr4g0423401 RchiOBHm_Chr6g0311831
rosa_laevigata	RLG00000007561
rosa_multiflora	Rmu_sc0002752.1_g000004 Rmu_ssc0000174.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365600
rosa_rugosa	Rorug04G0187300
rosa_samantha	Rh1BG304700 Rh3AG236600 Rh3DG262000 Rh4AG246200 Rh4AG246300 Rh4BG251700 Rh4CG260200 Rh4DG247100
rosa_wichuraiana	Rw4G021160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1019
Acc36I	ACCTGC	2 cut(s)	499, 1019
Acc65I	GGTACC	1 cut(s)	2423
AccB1I	GGYRCC	1 cut(s)	2423
AccI	GTMKAC	5 cut(s)	363, 731, 1295, 1717, 1786
AccII	CGCG	1 cut(s)	2250
AccIII	TCCGGA	2 cut(s)	1863, 2404
AciI	CCGC	9 cut(s)	40, 48, 51, 188, 321, 968, 2248, 2250, 2328
AclWI	GGATC	5 cut(s)	154, 296, 705, 1769, 1782
AcoI	YGGCCR	1 cut(s)	112
AcsI	RAATTY	2 cut(s)	230, 1274
AcuI	CTGAAG	3 cut(s)	612, 987, 1418
AcyI	GRCGYC	2 cut(s)	30, 484
AfaI	GTAC	4 cut(s)	298, 1878, 1956, 2425
AfiI	CCNNNNNNNGG	5 cut(s)	1109, 1206, 1305, 1562, 2287
AflIII	ACRYGT	1 cut(s)	1957
AgsI	TTSAA	7 cut(s)	245, 620, 1138, 1479, 1487, 1543, 1819
AjiI	CACGTC	1 cut(s)	1994
AjnI	CCWGG	5 cut(s)	234, 368, 1394, 1870, 2026
Alw21I	GWGCWC	2 cut(s)	15, 1344
Alw26I	GTCTC	3 cut(s)	64, 100, 2044
AlwI	GGATC	5 cut(s)	154, 296, 705, 1769, 1782
Aor13HI	TCCGGA	2 cut(s)	1863, 2404
AoxI	GGCC	6 cut(s)	112, 905, 1357, 2023, 2244, 2370
ApeKI	GCWGC	6 cut(s)	439, 604, 1391, 1823, 2063, 2210
ApoI	RAATTY	2 cut(s)	230, 1274
AseI	ATTAAT	1 cut(s)	1409
Asp700I	GAANNNNTTC	2 cut(s)	284, 849
Asp718I	GGTACC	1 cut(s)	2423
AspLEI	GCGC	5 cut(s)	354, 956, 1469, 1553, 2333
AspS9I	GGNCC	6 cut(s)	987, 1422, 2024, 2245, 2370, 2402
AsuC2I	CCSGG	2 cut(s)	192, 378
AsuHPI	GGTGA	4 cut(s)	82, 560, 1022, 1043
AvaII	GGWCC	3 cut(s)	987, 1422, 2402
BaeGI	GKGCMC	2 cut(s)	1021, 1894
BaeI	ACNNNNGTAYC	2 cut(s)	2407, 2440
BalI	TGGCCA	1 cut(s)	114
BamHI	GGATCC	1 cut(s)	1774
BanI	GGYRCC	1 cut(s)	2423
BanII	GRGCYC	4 cut(s)	15, 1344, 1531, 2101
BbsI	GAAGAC	1 cut(s)	2334
Bbv12I	GWGCWC	2 cut(s)	15, 1344
BbvI	GCAGC	6 cut(s)	426, 616, 1378, 1810, 2050, 2222
BccI	CCATC	5 cut(s)	978, 1114, 1412, 2290, 2393
BceAI	ACGGC	2 cut(s)	64, 521
BcgI	CGANNNNNNTGC	4 cut(s)	343, 377, 1802, 1836
BciT130I	CCWGG	5 cut(s)	236, 370, 1396, 1872, 2028
BcnI	CCSGG	2 cut(s)	192, 378
BcoDI	GTCTC	3 cut(s)	64, 100, 2044
BfaI	CTAG	3 cut(s)	470, 2180, 2258
BfmI	CTRYAG	3 cut(s)	264, 976, 2444
BfoI	RGCGCY	2 cut(s)	1470, 2334
BfuAI	ACCTGC	2 cut(s)	499, 1019
BlpI	GCTNAGC	1 cut(s)	2067
Bme1390I	CCNGG	7 cut(s)	192, 236, 370, 378, 1396, 1872, 2028
Bme18I	GGWCC	3 cut(s)	987, 1422, 2402
BmgBI	CACGTC	1 cut(s)	1994
BmgT120I	GGNCC	6 cut(s)	987, 1422, 2024, 2245, 2370, 2402
BmiI	GGNNCC	6 cut(s)	276, 319, 1423, 1530, 1776, 2425
BmrFI	CCNGG	7 cut(s)	192, 236, 370, 378, 1396, 1872, 2028
BmrI	ACTGGG	4 cut(s)	730, 843, 1194, 1991
BmuI	ACTGGG	4 cut(s)	730, 843, 1194, 1991
BoxI	GACNNNNGTC	1 cut(s)	2352
BpiI	GAAGAC	1 cut(s)	2334
BpmI	CTGGAG	2 cut(s)	1854, 2225
Bpu10I	CCTNAGC	1 cut(s)	1095
Bpu1102I	GCTNAGC	1 cut(s)	2067
BpuEI	CTTGAG	1 cut(s)	1652
BpuMI	CCSGG	2 cut(s)	192, 378
BsaAI	YACGTR	1 cut(s)	1958
BsaHI	GRCGYC	2 cut(s)	30, 484
BsaWI	WCCGGW	4 cut(s)	271, 1644, 1863, 2404
BsaXI	ACNNNNNCTCC	6 cut(s)	144, 174, 1316, 1346, 1565, 1595
Bsc4I	CCNNNNNNNGG	5 cut(s)	1109, 1206, 1305, 1562, 2287
Bse118I	RCCGGY	1 cut(s)	641
Bse1I	ACTGG	6 cut(s)	550, 725, 838, 1200, 1614, 1986
Bse3DI	GCAATG	5 cut(s)	1665, 2041, 2268, 2319, 2427
BseAI	TCCGGA	2 cut(s)	1863, 2404
BseBI	CCWGG	5 cut(s)	236, 370, 1396, 1872, 2028
BseGI	GGATG	5 cut(s)	685, 1099, 1315, 1679, 2413
BseLI	CCNNNNNNNGG	5 cut(s)	1109, 1206, 1305, 1562, 2287
BseMI	GCAATG	5 cut(s)	1665, 2041, 2268, 2319, 2427
BseMII	CTCAG	6 cut(s)	552, 872, 929, 1086, 1304, 2058
BseNI	ACTGG	6 cut(s)	550, 725, 838, 1200, 1614, 1986
BseRI	GAGGAG	2 cut(s)	1337, 1340
BseSI	GKGCMC	2 cut(s)	1021, 1894
BseXI	GCAGC	6 cut(s)	426, 616, 1378, 1810, 2050, 2222
BseYI	CCCAGC	2 cut(s)	1020, 1963
Bsh1236I	CGCG	1 cut(s)	2250
BshFI	GGCC	6 cut(s)	114, 907, 1359, 2025, 2246, 2372
BshNI	GGYRCC	1 cut(s)	2423
BsiHKAI	GWGCWC	2 cut(s)	15, 1344
BsiSI	CCGG	8 cut(s)	63, 191, 272, 377, 642, 1645, 1864, 2405
BslFI	GGGAC	5 cut(s)	671, 1161, 1181, 1435, 1946
BslI	CCNNNNNNNGG	5 cut(s)	1109, 1206, 1305, 1562, 2287
BsmAI	GTCTC	3 cut(s)	64, 100, 2044
BsmBI	CGTCTC	2 cut(s)	64, 100
BsmFI	GGGAC	5 cut(s)	671, 1161, 1181, 1435, 1946
BsmI	GAATGC	2 cut(s)	442, 711
BsnI	GGCC	6 cut(s)	114, 907, 1359, 2025, 2246, 2372
Bsp1286I	GDGCHC	6 cut(s)	15, 1021, 1344, 1531, 1894, 2101
Bsp13I	TCCGGA	2 cut(s)	1863, 2404
Bsp1407I	TGTACA	1 cut(s)	1876
Bsp143I	GATC	9 cut(s)	146, 301, 697, 805, 1162, 1249, 1506, 1774, 1848
Bsp1720I	GCTNAGC	1 cut(s)	2067
Bsp19I	CCATGG	4 cut(s)	902, 990, 1263, 2323
BspACI	CCGC	9 cut(s)	40, 48, 51, 188, 321, 968, 2248, 2250, 2328
BspANI	GGCC	6 cut(s)	114, 907, 1359, 2025, 2246, 2372
BspCNI	CTCAG	6 cut(s)	553, 871, 928, 1087, 1303, 2059
BspEI	TCCGGA	2 cut(s)	1863, 2404
BspFNI	CGCG	1 cut(s)	2250
BspLI	GGNNCC	6 cut(s)	276, 319, 1423, 1530, 1776, 2425
BspMI	ACCTGC	2 cut(s)	499, 1019
BspPI	GGATC	5 cut(s)	154, 296, 705, 1769, 1782
BspQI	GCTCTTC	1 cut(s)	892
BspT107I	GGYRCC	1 cut(s)	2423
BsrDI	GCAATG	5 cut(s)	1665, 2041, 2268, 2319, 2427
BsrFI	RCCGGY	1 cut(s)	641
BsrGI	TGTACA	1 cut(s)	1876
BsrI	ACTGG	6 cut(s)	550, 725, 838, 1200, 1614, 1986
BssAI	RCCGGY	1 cut(s)	641
BssMI	GATC	9 cut(s)	146, 301, 697, 805, 1162, 1249, 1506, 1774, 1848
BssNI	GRCGYC	2 cut(s)	30, 484
BssT1I	CCWWGG	4 cut(s)	902, 990, 1263, 2323
Bst2UI	CCWGG	5 cut(s)	236, 370, 1396, 1872, 2028
Bst4CI	ACNGT	7 cut(s)	94, 265, 767, 1312, 1547, 1842, 1900
Bst6I	CTCTTC	2 cut(s)	892, 2104
BstACI	GRCGYC	2 cut(s)	30, 484
BstAUI	TGTACA	1 cut(s)	1876
BstBAI	YACGTR	1 cut(s)	1958
BstC8I	GCNNGC	9 cut(s)	390, 394, 557, 970, 1026, 1344, 1932, 2248, 2335
BstDSI	CCRYGG	6 cut(s)	902, 949, 990, 1263, 2248, 2323
BstENI	CCTNNNNNAGG	2 cut(s)	1107, 1560
BstF5I	GGATG	5 cut(s)	685, 1099, 1315, 1679, 2413
BstFNI	CGCG	1 cut(s)	2250
BstH2I	RGCGCY	2 cut(s)	1470, 2334
BstHHI	GCGC	5 cut(s)	354, 956, 1469, 1553, 2333
BstKTI	GATC	9 cut(s)	149, 304, 700, 808, 1165, 1252, 1509, 1777, 1851
BstMAI	GTCTC	3 cut(s)	64, 100, 2044
BstMBI	GATC	9 cut(s)	146, 301, 697, 805, 1162, 1249, 1506, 1774, 1848
BstMWI	GCNNNNNNNGC	6 cut(s)	1025, 1096, 1397, 2096, 2273, 2334
BstNI	CCWGG	5 cut(s)	236, 370, 1396, 1872, 2028
BstNSI	RCATGY	2 cut(s)	1028, 1934
BstPAI	GACNNNNGTC	1 cut(s)	2352
BstSCI	CCNGG	7 cut(s)	190, 234, 368, 376, 1394, 1870, 2026
BstSFI	CTRYAG	3 cut(s)	264, 976, 2444
BstSLI	GKGCMC	2 cut(s)	1021, 1894
BstUI	CGCG	1 cut(s)	2250
BstV1I	GCAGC	6 cut(s)	426, 616, 1378, 1810, 2050, 2222
BstV2I	GAAGAC	1 cut(s)	2334
BstX2I	RGATCY	1 cut(s)	1774
BstXI	CCANNNNNNTGG	1 cut(s)	2197
BstYI	RGATCY	1 cut(s)	1774
BsuRI	GGCC	6 cut(s)	114, 907, 1359, 2025, 2246, 2372
BtgI	CCRYGG	6 cut(s)	902, 949, 990, 1263, 2248, 2323
BtgZI	GCGATG	1 cut(s)	420
BtrI	CACGTC	1 cut(s)	1994
BtsCI	GGATG	5 cut(s)	685, 1099, 1315, 1679, 2413
BtsIMutI	CAGTG	4 cut(s)	165, 543, 923, 1317
BveI	ACCTGC	2 cut(s)	499, 1019
Cac8I	GCNNGC	9 cut(s)	390, 394, 557, 970, 1026, 1344, 1932, 2248, 2335
CfoI	GCGC	5 cut(s)	354, 956, 1469, 1553, 2333
Cfr10I	RCCGGY	1 cut(s)	641
Cfr13I	GGNCC	6 cut(s)	987, 1422, 2024, 2245, 2370, 2402
Cfr42I	CCGCGG	1 cut(s)	2251
CseI	GACGC	3 cut(s)	38, 144, 473
Csp6I	GTAC	4 cut(s)	297, 1877, 1955, 2424
CviQI	GTAC	4 cut(s)	297, 1877, 1955, 2424
DpnI	GATC	9 cut(s)	148, 303, 699, 807, 1164, 1251, 1508, 1776, 1850
DpnII	GATC	9 cut(s)	146, 301, 697, 805, 1162, 1249, 1506, 1774, 1848
EaeI	YGGCCR	1 cut(s)	112
Eam1104I	CTCTTC	2 cut(s)	892, 2104
EarI	CTCTTC	2 cut(s)	892, 2104
Ecl136II	GAGCTC	2 cut(s)	13, 1342
Eco130I	CCWWGG	4 cut(s)	902, 990, 1263, 2323
Eco147I	AGGCCT	1 cut(s)	1359
Eco24I	GRGCYC	4 cut(s)	15, 1344, 1531, 2101
Eco32I	GATATC	2 cut(s)	1496, 1861
Eco47I	GGWCC	3 cut(s)	987, 1422, 2402
Eco53kI	GAGCTC	2 cut(s)	13, 1342
Eco57I	CTGAAG	3 cut(s)	612, 987, 1418
EcoICRI	GAGCTC	2 cut(s)	13, 1342
EcoNI	CCTNNNNNAGG	2 cut(s)	1107, 1560
EcoRI	GAATTC	2 cut(s)	230, 1274
EcoRII	CCWGG	5 cut(s)	234, 368, 1394, 1870, 2026
EcoRV	GATATC	2 cut(s)	1496, 1861
EcoT14I	CCWWGG	4 cut(s)	902, 990, 1263, 2323
EcoT22I	ATGCAT	2 cut(s)	1063, 1833
EcoT38I	GRGCYC	4 cut(s)	15, 1344, 1531, 2101
ErhI	CCWWGG	4 cut(s)	902, 990, 1263, 2323
Esp3I	CGTCTC	2 cut(s)	64, 100
FalI	AAGNNNNNCTT	2 cut(s)	795, 827
FaqI	GGGAC	5 cut(s)	671, 1161, 1181, 1435, 1946
FauI	CCCGC	2 cut(s)	961, 2255
FblI	GTMKAC	5 cut(s)	363, 731, 1295, 1717, 1786
FokI	GGATG	5 cut(s)	672, 1086, 1302, 1666, 2420
FriOI	GRGCYC	4 cut(s)	15, 1344, 1531, 2101
FspBI	CTAG	3 cut(s)	470, 2180, 2258
GlaI	GCGC	5 cut(s)	353, 955, 1468, 1552, 2332
GsaI	CCCAGC	2 cut(s)	1024, 1967
GsuI	CTGGAG	2 cut(s)	1854, 2225
HaeII	RGCGCY	2 cut(s)	1470, 2334
HaeIII	GGCC	6 cut(s)	114, 907, 1359, 2025, 2246, 2372
HapII	CCGG	8 cut(s)	63, 191, 272, 377, 642, 1645, 1864, 2405
HgaI	GACGC	3 cut(s)	38, 144, 473
HhaI	GCGC	5 cut(s)	354, 956, 1469, 1553, 2333
Hin1I	GRCGYC	2 cut(s)	30, 484
Hin6I	GCGC	5 cut(s)	352, 954, 1467, 1551, 2331
HinP1I	GCGC	5 cut(s)	352, 954, 1467, 1551, 2331
HincII	GTYRAC	2 cut(s)	364, 799
HindII	GTYRAC	2 cut(s)	364, 799
HindIII	AAGCTT	1 cut(s)	426
HinfI	GANTC	7 cut(s)	121, 623, 850, 1082, 1641, 1727, 1867
HpaII	CCGG	8 cut(s)	63, 191, 272, 377, 642, 1645, 1864, 2405
HphI	GGTGA	4 cut(s)	82, 560, 1022, 1043
Hpy99I	CGWCG	2 cut(s)	32, 489
HpyAV	CCTTC	8 cut(s)	341, 545, 839, 864, 1172, 1349, 1982, 2025
HpyCH4III	ACNGT	7 cut(s)	94, 265, 767, 1312, 1547, 1842, 1900
HpyCH4IV	ACGT	5 cut(s)	57, 171, 1303, 1957, 1993
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1025, 1096, 1397, 2096, 2273, 2334
HpySE526I	ACGT	5 cut(s)	57, 171, 1303, 1957, 1993
Hsp92I	GRCGYC	2 cut(s)	30, 484
HspAI	GCGC	5 cut(s)	352, 954, 1467, 1551, 2331
Kpn2I	TCCGGA	2 cut(s)	1863, 2404
KpnI	GGTACC	1 cut(s)	2427
KspI	CCGCGG	1 cut(s)	2251
Kzo9I	GATC	9 cut(s)	146, 301, 697, 805, 1162, 1249, 1506, 1774, 1848
LguI	GCTCTTC	1 cut(s)	892
LmnI	GCTCC	5 cut(s)	42, 152, 1339, 1534, 2060
Lsp1109I	GCAGC	6 cut(s)	426, 616, 1378, 1810, 2050, 2222
MaeI	CTAG	3 cut(s)	470, 2180, 2258
MaeII	ACGT	5 cut(s)	57, 171, 1303, 1957, 1993
MaeIII	GTNAC	3 cut(s)	597, 1031, 2034
MalI	GATC	9 cut(s)	148, 303, 699, 807, 1164, 1251, 1508, 1776, 1850
MboI	GATC	9 cut(s)	146, 301, 697, 805, 1162, 1249, 1506, 1774, 1848
MboII	GAAGA	9 cut(s)	233, 586, 618, 632, 879, 1172, 1259, 2121, 2334
MflI	RGATCY	1 cut(s)	1774
MhlI	GDGCHC	6 cut(s)	15, 1021, 1344, 1531, 1894, 2101
MlsI	TGGCCA	1 cut(s)	114
MluCI	AATT	9 cut(s)	85, 230, 725, 960, 1042, 1217, 1274, 1406, 1410
MluNI	TGGCCA	1 cut(s)	114
MlyI	GAGTC	1 cut(s)	1861
MmeI	TCCRAC	2 cut(s)	149, 1286
Mox20I	TGGCCA	1 cut(s)	114
Mph1103I	ATGCAT	2 cut(s)	1063, 1833
MroI	TCCGGA	2 cut(s)	1863, 2404
MroXI	GAANNNNTTC	2 cut(s)	284, 849
MscI	TGGCCA	1 cut(s)	114
MseI	TTAA	2 cut(s)	1334, 1409
MslI	CAYNNNNRTG	3 cut(s)	1029, 1929, 2312
Msp20I	TGGCCA	1 cut(s)	114
MspA1I	CMGCKG	1 cut(s)	2250
MspI	CCGG	8 cut(s)	63, 191, 272, 377, 642, 1645, 1864, 2405
MspR9I	CCNGG	7 cut(s)	192, 236, 370, 378, 1396, 1872, 2028
Mva1269I	GAATGC	2 cut(s)	442, 711
MvaI	CCWGG	5 cut(s)	236, 370, 1396, 1872, 2028
MvnI	CGCG	1 cut(s)	2250
MwoI	GCNNNNNNNGC	6 cut(s)	1025, 1096, 1397, 2096, 2273, 2334
NciI	CCSGG	2 cut(s)	192, 378
NcoI	CCATGG	4 cut(s)	902, 990, 1263, 2323
NdeII	GATC	9 cut(s)	146, 301, 697, 805, 1162, 1249, 1506, 1774, 1848
NlaIV	GGNNCC	6 cut(s)	276, 319, 1423, 1530, 1776, 2425
NmuCI	GTSAC	1 cut(s)	1031
NsiI	ATGCAT	2 cut(s)	1063, 1833
NspI	RCATGY	2 cut(s)	1028, 1934
PaeI	GCATGC	2 cut(s)	1028, 1934
PaqCI	CACCTGC	1 cut(s)	1019
PceI	AGGCCT	1 cut(s)	1359
PciSI	GCTCTTC	1 cut(s)	892
PctI	GAATGC	2 cut(s)	442, 711
PdmI	GAANNNNTTC	2 cut(s)	284, 849
PfeI	GAWTC	6 cut(s)	121, 623, 850, 1082, 1641, 1727
PleI	GAGTC	1 cut(s)	1861
PpsI	GAGTC	1 cut(s)	1861
Ppu21I	YACGTR	1 cut(s)	1958
PshAI	GACNNNNGTC	1 cut(s)	2352
PshBI	ATTAAT	1 cut(s)	1409
Psp124BI	GAGCTC	2 cut(s)	15, 1344
Psp6I	CCWGG	5 cut(s)	234, 368, 1394, 1870, 2026
PspFI	CCCAGC	2 cut(s)	1020, 1963
PspGI	CCWGG	5 cut(s)	234, 368, 1394, 1870, 2026
PspN4I	GGNNCC	6 cut(s)	276, 319, 1423, 1530, 1776, 2425
PspPI	GGNCC	6 cut(s)	987, 1422, 2024, 2245, 2370, 2402
PsrI	GAACNNNNNNTAC	2 cut(s)	258, 290
PsuI	RGATCY	1 cut(s)	1774
RsaI	GTAC	4 cut(s)	298, 1878, 1956, 2425
RsaNI	GTAC	4 cut(s)	297, 1877, 1955, 2424
RseI	CAYNNNNRTG	3 cut(s)	1029, 1929, 2312
SacI	GAGCTC	2 cut(s)	15, 1344
SacII	CCGCGG	1 cut(s)	2251
SalI	GTCGAC	1 cut(s)	362
SapI	GCTCTTC	1 cut(s)	892
SaqAI	TTAA	2 cut(s)	1334, 1409
Sau3AI	GATC	9 cut(s)	146, 301, 697, 805, 1162, 1249, 1506, 1774, 1848
Sau96I	GGNCC	6 cut(s)	987, 1422, 2024, 2245, 2370, 2402
SchI	GAGTC	1 cut(s)	1861
ScrFI	CCNGG	7 cut(s)	192, 236, 370, 378, 1396, 1872, 2028
SduI	GDGCHC	6 cut(s)	15, 1021, 1344, 1531, 1894, 2101
SfcI	CTRYAG	3 cut(s)	264, 976, 2444
Sfr303I	CCGCGG	1 cut(s)	2251
SgrBI	CCGCGG	1 cut(s)	2251
SinI	GGWCC	3 cut(s)	987, 1422, 2402
SmiMI	CAYNNNNRTG	3 cut(s)	1029, 1929, 2312
SmlI	CTYRAG	1 cut(s)	1631
SmoI	CTYRAG	1 cut(s)	1631
SphI	GCATGC	2 cut(s)	1028, 1934
Sse9I	AATT	9 cut(s)	85, 230, 725, 960, 1042, 1217, 1274, 1406, 1410
SseBI	AGGCCT	1 cut(s)	1359
SsiI	CCGC	9 cut(s)	40, 48, 51, 188, 321, 968, 2248, 2250, 2328
SspI	AATATT	1 cut(s)	1975
SspMI	CTAG	3 cut(s)	470, 2180, 2258
SstI	GAGCTC	2 cut(s)	15, 1344
StuI	AGGCCT	1 cut(s)	1359
StyD4I	CCNGG	7 cut(s)	190, 234, 368, 376, 1394, 1870, 2026
StyI	CCWWGG	4 cut(s)	902, 990, 1263, 2323
TaaI	ACNGT	7 cut(s)	94, 265, 767, 1312, 1547, 1842, 1900
TaiI	ACGT	5 cut(s)	60, 174, 1306, 1960, 1996
TaqI	TCGA	9 cut(s)	15, 21, 27, 119, 363, 669, 700, 1701, 2170
TasI	AATT	9 cut(s)	85, 230, 725, 960, 1042, 1217, 1274, 1406, 1410
TatI	WGTACW	2 cut(s)	296, 1876
TauI	GCSGC	4 cut(s)	50, 53, 190, 2331
TfiI	GAWTC	6 cut(s)	121, 623, 850, 1082, 1641, 1727
Tru1I	TTAA	2 cut(s)	1334, 1409
Tru9I	TTAA	2 cut(s)	1334, 1409
TscAI	CASTG	4 cut(s)	165, 550, 923, 1317
TseFI	GTSAC	1 cut(s)	1031
TseI	GCWGC	6 cut(s)	439, 604, 1391, 1823, 2063, 2210
Tsp45I	GTSAC	1 cut(s)	1031
TspDTI	ATGAA	5 cut(s)	674, 879, 1231, 1719, 2424
TspGWI	ACGGA	2 cut(s)	938, 1506
TspRI	CASTG	4 cut(s)	165, 550, 923, 1317
VpaK11BI	GGWCC	3 cut(s)	987, 1422, 2402
VspI	ATTAAT	1 cut(s)	1409
XagI	CCTNNNNNAGG	2 cut(s)	1107, 1560
XapI	RAATTY	2 cut(s)	230, 1274
XceI	RCATGY	2 cut(s)	1028, 1934
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2330
XmiI	GTMKAC	5 cut(s)	363, 731, 1295, 1717, 1786
XmnI	GAANNNNTTC	2 cut(s)	284, 849
XspI	CTAG	3 cut(s)	470, 2180, 2258
Zsp2I	ATGCAT	2 cut(s)	1063, 1833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.