Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003931

Physical Location & Seq

Genomic Coordinates Forward (+)

13563463 .. 13569129

Gene Structure 5667 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1164100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGGATCAGAGCCGTGCGCCTCTCGATTTCGACCTCAACTCCTTGCTATCCTACGCCTCCGCCAACGTTCTTGGCTTCCCTCCCTCTCCCTCCAATTTCACAGTCTCTAAGTTCGGGCATGGGCAATCGAATCCGACGTATAAGATAGAGGTAGGGTCGGGGGCATCGCTGAAACGCTACGTTTTGAGGAAGAAGCCGCCGGGGAAGTTGCTCCAGTCTGCTCATGCCGTCGAGAGAGAGTTCCAGGTTCTTCAGGCATTGGGTAATCATACATTGGTTCCAGTTCCAAAAGTTTACTGTTTATGTACCGATTCAGGGGTCATCGGAACCCCCTTTTACATCATGGAGTTTCTGGAAGGGCGCATTTTTCTCGACCCCAAGCTTCCGGGTGTAGCACCTGAGAGGAGGAGGGTACTTTACCAAGCAACTGCAAGAGCTCTAGCATCCCTACATTCTGCTGATGTTGATGCCATCGGTCTAGGAAAATATGGTCGTCGTGACAACTATTGCAAACGGCAGGTAGAGAGGTGGGCGAAGCAATATATTGCCTCAACTGGTGAGGGCAAGCCCAAGAGAAATCCGAAGATGTTTGAGCTGATTGATTGGTTACAGCAGAACATTCCTCTTGAAGATTCTTCTGGGACAGCAGCGGGCTTAGTTCATGGGGACTTTCGCATTGATAATCTTGTGTTTCATCCTATTGAGGATCAAGTGATTGGCATTCTTGACTGGGAATTATCTACTCTTGGAAATCAAATGTGTGATGTTGCTTATAGCTGTTTGCCTTACATCGTGGACATCGGGGCTGAAGTTGGTGAAGGTTTGGAACACACGGGGGTTCCAGAAGGGATTCCTTCTCAGGCAGAGTATGTAGCAGAATACTGTTCTTCATCAGGAAAGCCATGGCCTTTTGCTGAGTGGAAGTTCTATGTTGCCTTCTCCTTATTTCGAGGGGCATCAATTTTTGCAGGGATATATAGTAGATGGATTATGGGTAATGCTTCTGGAGGAGAGAGTGCCCAGCATGCTGGGGATAGAGCTAATTTTATTATAGACTATGCATGGAAATTTATTCGACAAGAATCTGTGCTTCCCAAGAATCCTCCGTCAGGTGCTTCCCTTGCTCAAGACTACTCGAAAAGATTTGGACAAGAGAGTGATGATCAAGGATTTTCAAAAGGGGGAGGGAGGTTTGTCCCCAGTAAAAGGATTTCAGAATTGAGAAACAGGTTGATAAAGTTCATGGAAGATCACATTTATCCCATGGAACAAGAATTCAACAAACTTGCTGAGTCTAGCTCACGTTGGACGGTTCATCCAGGGGAGGAGAGGTTAAAGGAGCTAGCAAAGAAAGAAGGCCTCTGGAACTTATTTATACCTTTTGACAGTGCTGCCAGGGCCAGAAAATTAATTTTTGATGGAAGCAATCATCTTTTATCAGAGAATTCCTCCAATCACTTATTGGGCGCAGGTCTTTCAAACCTTGAATACGGATATCTTTGTGAGATCATGGGTCGTTCAGTTTGGGCTCCACAAGTGTTCAATTGTGGTGCACCTGACACGGGAAATATGGAGGTATTGTTGCGCTATGGCAGCAAGGAGCAGCTACTTGAATGGCTTGTTCCTTTGCTTGAGGGGAAAATTCGGTCAGGATTCGCAATGACGGAACCCCAAGTTGCATCCTCTGATGCGACCAATATCGAGTGTTCTATTAAAAGAGAAGGAGATTCATATATCATCAACGGAAAGAAATGGTGGACAAGTGGTGCTATGGATCCAAGGTGTAGAGTTCTTATAGTCATGGGGAAAACCGACTTCAATGCTTCCATGCATAAGCAGCAATCTATGATCTTAGTGGATATCCAAACTCCAGGTGTACACATAAAGAGACCACTTACAGTTTTTGGCTTTGATGATGCACCTCATGGGCATGCAGAAGTATTATTTGAGAATGTACGTGTCCCAGCAAAAAATATTCTACTTGGAGAAGGACGTGGGTTTGAAATCGCACAGGGTAGACTAGGCCCAGGAAGGTTACACCATTGCATGAGGCTGATAGGAGCTGCCGAGCGTGGCATCTGGCTAATGGCTCAAAGGGCTCTTAGTAGAAAAGTTTTTGGGAAGTTGATTGCTGAACAAGGCTCATTTCTTTCAGATATTGCAAAGTGTCGAATTGAGCTAGAGAAAGCCAGATTGTTGGTTCTGGAGGCAGCTGACCAGCTCGATAGACTTGGAAACAAAAAGGCCCGAGGAACCATAGCAATGGCAAAGGTAGCGGCCCCAAACATGGCGCTAATGGTGCTCGACATGGCAATGCAAGTGCACGGTGGCGCTGGCTTGTCTTCCGACACTTGTCTGGCACATCTTTGGGCCACGGCAAGAACATTGAGAATCGCAGATGGTCCAGATGAAGTCCACTTGGGTACTATCGCCAAGTTAGAACTACGGAGAGCCAAACTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003995 GO:0005488 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016042 GO:0016049 GO:0016054 GO:0016491 GO:0016627 GO:0019395 GO:0019752 GO:0021700 GO:0022622 GO:0030154 GO:0030258 GO:0032501 GO:0032502 GO:0032787 GO:0033539 GO:0034440 GO:0036094 GO:0040007 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0046395 GO:0048037 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050660 GO:0050662 GO:0055114 GO:0060560 GO:0071695 GO:0071704 GO:0072329 GO:0080147 GO:0090558 GO:0090627 GO:0097159 GO:0099402 GO:1901265 GO:1901363 GO:1901575 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko00071 ko00280 ko00410 ko00640 ko01100 ko01110 ko01130 ko01200 ko01212 ko03320 map00071 map00280 map00410 map00640 map01100 map01110 map01130 map01200 map01212 map03320

Pfam Domains

Protein Families

APH Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

821

Amino Acids

90.79

Weight (kDa)

8.38

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APH	PF01636	35 - 277	3e-48	Phosphotransferase enzyme family
Acyl-CoA_dh_M	PF02770	551 - 652	4.1e-18	Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1	PF00441	664 - 812	1e-34	Acyl-CoA dehydrogenase, C-terminal domain
Acyl-CoA_dh_2	PF08028	680 - 790	5.4e-07	Acyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06810
fragaria_vesca	FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700 FvH4_4g18700
malus_domestica	MD13G1164300.v1.1 MD16G1164100.v1.1
prunus_persica	Prupe.1G131800_v2.0.a1 Prupe.1G131800_v2.0.a1 Prupe.1G131800_v2.0.a1
pyrus_communis	pycom13g14030 pycom16g13880 pycom16g13890
rosa_chinensis	RchiOBHm_Chr3g0480631 RchiOBHm_Chr4g0423401 RchiOBHm_Chr6g0311831
rosa_laevigata	RLG00000007561
rosa_multiflora	Rmu_sc0002752.1_g000004 Rmu_ssc0000174.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365600
rosa_rugosa	Rorug04G0187300
rosa_samantha	Rh1BG304700 Rh3AG236600 Rh3DG262000 Rh4AG246200 Rh4AG246300 Rh4BG251700 Rh4CG260200 Rh4DG247100
rosa_wichuraiana	Rw4G021160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	508, 1460
AccI	GTMKAC	1 cut(s)	2017
AciI	CCGC	4 cut(s)	60, 197, 650, 2276
AclI	AACGTT	1 cut(s)	66
AclWI	GGATC	4 cut(s)	12, 714, 1769, 1782
AcsI	RAATTY	4 cut(s)	1067, 1274, 1444, 1641
AcuI	CTGAAG	2 cut(s)	236, 828
AfaI	GTAC	5 cut(s)	307, 414, 1878, 1956, 2425
AfiI	CCNNNNNNNGG	5 cut(s)	315, 1109, 1206, 1562, 2287
AflIII	ACRYGT	1 cut(s)	1957
AgsI	TTSAA	9 cut(s)	629, 1176, 1279, 1479, 1487, 1543, 1613, 1819, 2003
AjiI	CACGTC	1 cut(s)	1994
AjnI	CCWGG	5 cut(s)	243, 1318, 1394, 1870, 2026
Alw21I	GWGCWC	4 cut(s)	439, 1555, 2304, 2325
Alw26I	GTCTC	2 cut(s)	109, 1882
Alw44I	GTGCAC	2 cut(s)	1551, 2321
AlwI	GGATC	4 cut(s)	12, 714, 1769, 1782
Ama87I	CYCGRG	1 cut(s)	2247
AoxI	GGCC	7 cut(s)	905, 1357, 1398, 2023, 2244, 2277, 2370
ApaLI	GTGCAC	2 cut(s)	1551, 2321
ApeKI	GCWGC	7 cut(s)	647, 1391, 1593, 1603, 1837, 2063, 2210
ApoI	RAATTY	4 cut(s)	1067, 1274, 1444, 1641
AseI	ATTAAT	1 cut(s)	1409
Asp700I	GAANNNNTTC	1 cut(s)	849
AspLEI	GCGC	6 cut(s)	19, 363, 1469, 1587, 2293, 2333
AspS9I	GGNCC	6 cut(s)	1398, 2024, 2245, 2278, 2370, 2402
AsuC2I	CCSGG	2 cut(s)	201, 387
AsuHPI	GGTGA	2 cut(s)	569, 827
AsuNHI	GCTAGC	1 cut(s)	1342
AvaI	CYCGRG	1 cut(s)	2247
AvaII	GGWCC	1 cut(s)	2402
BaeGI	GKGCMC	3 cut(s)	1021, 1555, 2325
BamHI	GGATCC	1 cut(s)	1774
BanII	GRGCYC	3 cut(s)	439, 1531, 2101
BbsI	GAAGAC	1 cut(s)	2334
Bbv12I	GWGCWC	4 cut(s)	439, 1555, 2304, 2325
BbvI	GCAGC	7 cut(s)	659, 1378, 1605, 1615, 1849, 2050, 2222
BccI	CCATC	4 cut(s)	479, 978, 1412, 2393
BceAI	ACGGC	3 cut(s)	212, 530, 2391
BcgI	CGANNNNNNTGC	4 cut(s)	352, 386, 1802, 1836
BciT130I	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
BclI	TGATCA	1 cut(s)	1162
BcnI	CCSGG	2 cut(s)	201, 387
BcoDI	GTCTC	2 cut(s)	109, 1882
BfaI	CTAG	6 cut(s)	440, 479, 1296, 1343, 2021, 2180
BfoI	RGCGCY	2 cut(s)	2294, 2334
BfuAI	ACCTGC	2 cut(s)	508, 1460
BisI	GCNGC	9 cut(s)	197, 648, 1392, 1594, 1604, 1838, 2064, 2211, 2277
BlsI	GCNGC	9 cut(s)	198, 649, 1393, 1595, 1605, 1839, 2065, 2212, 2278
Bme1390I	CCNGG	7 cut(s)	201, 245, 387, 1320, 1396, 1872, 2028
Bme18I	GGWCC	1 cut(s)	2402
BmeT110I	CYCGRG	1 cut(s)	2247
BmgBI	CACGTC	1 cut(s)	1994
BmgT120I	GGNCC	6 cut(s)	1398, 2024, 2245, 2278, 2370, 2402
BmiI	GGNNCC	8 cut(s)	279, 328, 840, 1530, 1668, 1776, 2254, 2280
BmrFI	CCNGG	7 cut(s)	201, 245, 387, 1320, 1396, 1872, 2028
BmrI	ACTGGG	2 cut(s)	739, 1194
BmsI	GCATC	8 cut(s)	173, 452, 457, 965, 1678, 1688, 1906, 2085
BmtI	GCTAGC	1 cut(s)	1346
BmuI	ACTGGG	2 cut(s)	739, 1194
BoxI	GACNNNNGTC	1 cut(s)	2352
BpiI	GAAGAC	1 cut(s)	2334
BplI	GAGNNNNNCTC	5 cut(s)	1283, 1315, 1433, 1465, 2442
BpmI	CTGGAG	4 cut(s)	197, 1026, 1854, 2225
BpuEI	CTTGAG	2 cut(s)	1110, 1652
BpuMI	CCSGG	2 cut(s)	201, 387
BsaAI	YACGTR	1 cut(s)	1958
BsaI	GGTCTC	1 cut(s)	1882
BsaJI	CCNNGG	9 cut(s)	200, 902, 1263, 1319, 1395, 1778, 2026, 2248, 2373
BsaXI	ACNNNNNCTCC	6 cut(s)	23, 53, 1316, 1346, 1565, 1595
Bsc4I	CCNNNNNNNGG	5 cut(s)	315, 1109, 1206, 1562, 2287
Bse1I	ACTGG	5 cut(s)	214, 281, 559, 734, 1200
Bse3DI	GCAATG	4 cut(s)	1665, 2041, 2268, 2319
BseBI	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
BseDI	CCNNGG	9 cut(s)	200, 902, 1263, 1319, 1395, 1778, 2026, 2248, 2373
BseGI	GGATG	4 cut(s)	443, 694, 1315, 1679
BseLI	CCNNNNNNNGG	5 cut(s)	315, 1109, 1206, 1562, 2287
BseMI	GCAATG	4 cut(s)	1665, 2041, 2268, 2319
BseMII	CTCAG	4 cut(s)	390, 872, 906, 1281
BseNI	ACTGG	5 cut(s)	214, 281, 559, 734, 1200
BseRI	GAGGAG	4 cut(s)	418, 421, 1023, 1340
BseSI	GKGCMC	3 cut(s)	1021, 1555, 2325
BseXI	GCAGC	7 cut(s)	659, 1378, 1605, 1615, 1849, 2050, 2222
BseYI	CCCAGC	3 cut(s)	1020, 1028, 1963
BshFI	GGCC	7 cut(s)	907, 1359, 1400, 2025, 2246, 2279, 2372
BsiHKAI	GWGCWC	4 cut(s)	439, 1555, 2304, 2325
BsiHKCI	CYCGRG	1 cut(s)	2247
BsiSI	CCGG	2 cut(s)	200, 386
BslFI	GGGAC	4 cut(s)	655, 680, 1181, 1946
BslI	CCNNNNNNNGG	5 cut(s)	315, 1109, 1206, 1562, 2287
BsmAI	GTCTC	2 cut(s)	109, 1882
BsmFI	GGGAC	4 cut(s)	655, 680, 1181, 1946
BsmI	GAATGC	1 cut(s)	720
BsnI	GGCC	7 cut(s)	907, 1359, 1400, 2025, 2246, 2279, 2372
Bso31I	GGTCTC	1 cut(s)	1882
BsoBI	CYCGRG	1 cut(s)	2247
Bsp1286I	GDGCHC	7 cut(s)	439, 1021, 1531, 1555, 2101, 2304, 2325
Bsp1407I	TGTACA	1 cut(s)	1876
Bsp143I	GATC	7 cut(s)	4, 706, 1162, 1249, 1506, 1774, 1848
Bsp19I	CCATGG	2 cut(s)	902, 1263
BspACI	CCGC	4 cut(s)	60, 197, 650, 2276
BspANI	GGCC	7 cut(s)	907, 1359, 1400, 2025, 2246, 2279, 2372
BspCNI	CTCAG	4 cut(s)	391, 871, 907, 1282
BspLI	GGNNCC	8 cut(s)	279, 328, 840, 1530, 1668, 1776, 2254, 2280
BspMI	ACCTGC	2 cut(s)	508, 1460
BspOI	GCTAGC	1 cut(s)	1346
BspPI	GGATC	4 cut(s)	12, 714, 1769, 1782
BspTNI	GGTCTC	1 cut(s)	1882
BsrDI	GCAATG	4 cut(s)	1665, 2041, 2268, 2319
BsrGI	TGTACA	1 cut(s)	1876
BsrI	ACTGG	5 cut(s)	214, 281, 559, 734, 1200
BssECI	CCNNGG	9 cut(s)	200, 902, 1263, 1319, 1395, 1778, 2026, 2248, 2373
BssMI	GATC	7 cut(s)	4, 706, 1162, 1249, 1506, 1774, 1848
BssT1I	CCWWGG	3 cut(s)	902, 1263, 1778
Bst2UI	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
Bst4CI	ACNGT	7 cut(s)	103, 299, 884, 1312, 1388, 1900, 2327
BstAUI	TGTACA	1 cut(s)	1876
BstBAI	YACGTR	1 cut(s)	1958
BstC8I	GCNNGC	6 cut(s)	566, 652, 1026, 1344, 1932, 2335
BstDEI	CTNAG	8 cut(s)	108, 399, 655, 858, 915, 1290, 1852, 2102
BstDSI	CCRYGG	3 cut(s)	902, 1263, 2373
BstENI	CCTNNNNNAGG	1 cut(s)	1107
BstF5I	GGATG	4 cut(s)	443, 694, 1315, 1679
BstH2I	RGCGCY	2 cut(s)	2294, 2334
BstHHI	GCGC	6 cut(s)	19, 363, 1469, 1587, 2293, 2333
BstKTI	GATC	7 cut(s)	7, 709, 1165, 1252, 1509, 1777, 1851
BstMAI	GTCTC	2 cut(s)	109, 1882
BstMBI	GATC	7 cut(s)	4, 706, 1162, 1249, 1506, 1774, 1848
BstMWI	GCNNNNNNNGC	8 cut(s)	1025, 1121, 1397, 1593, 1837, 2096, 2273, 2299
BstNI	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
BstNSI	RCATGY	2 cut(s)	1028, 1934
BstPAI	GACNNNNGTC	1 cut(s)	2352
BstSCI	CCNGG	7 cut(s)	199, 243, 385, 1318, 1394, 1870, 2026
BstSLI	GKGCMC	3 cut(s)	1021, 1555, 2325
BstV1I	GCAGC	7 cut(s)	659, 1378, 1605, 1615, 1849, 2050, 2222
BstV2I	GAAGAC	1 cut(s)	2334
BstX2I	RGATCY	1 cut(s)	1774
BstXI	CCANNNNNNTGG	3 cut(s)	1028, 2197, 2263
BstYI	RGATCY	1 cut(s)	1774
BsuRI	GGCC	7 cut(s)	907, 1359, 1400, 2025, 2246, 2279, 2372
BtgI	CCRYGG	3 cut(s)	902, 1263, 2373
BtgZI	GCGATG	1 cut(s)	150
BtrI	CACGTC	1 cut(s)	1994
BtsCI	GGATG	4 cut(s)	443, 694, 1315, 1679
BtsIMutI	CAGTG	1 cut(s)	1393
BveI	ACCTGC	2 cut(s)	508, 1460
Cac8I	GCNNGC	6 cut(s)	566, 652, 1026, 1344, 1932, 2335
CfoI	GCGC	6 cut(s)	19, 363, 1469, 1587, 2293, 2333
Cfr13I	GGNCC	6 cut(s)	1398, 2024, 2245, 2278, 2370, 2402
Csp6I	GTAC	5 cut(s)	306, 413, 1877, 1955, 2424
CviQI	GTAC	5 cut(s)	306, 413, 1877, 1955, 2424
DdeI	CTNAG	8 cut(s)	108, 399, 655, 858, 915, 1290, 1852, 2102
DpnI	GATC	7 cut(s)	6, 708, 1164, 1251, 1508, 1776, 1850
DpnII	GATC	7 cut(s)	4, 706, 1162, 1249, 1506, 1774, 1848
EciI	GGCGGA	1 cut(s)	49
Ecl136II	GAGCTC	1 cut(s)	437
Eco130I	CCWWGG	3 cut(s)	902, 1263, 1778
Eco147I	AGGCCT	1 cut(s)	1359
Eco24I	GRGCYC	3 cut(s)	439, 1531, 2101
Eco31I	GGTCTC	1 cut(s)	1882
Eco32I	GATATC	2 cut(s)	1496, 1861
Eco47I	GGWCC	1 cut(s)	2402
Eco53kI	GAGCTC	1 cut(s)	437
Eco57I	CTGAAG	2 cut(s)	236, 828
Eco88I	CYCGRG	1 cut(s)	2247
EcoICRI	GAGCTC	1 cut(s)	437
EcoNI	CCTNNNNNAGG	1 cut(s)	1107
EcoRI	GAATTC	2 cut(s)	1274, 1444
EcoRII	CCWGG	5 cut(s)	243, 1318, 1394, 1870, 2026
EcoRV	GATATC	2 cut(s)	1496, 1861
EcoT14I	CCWWGG	3 cut(s)	902, 1263, 1778
EcoT22I	ATGCAT	2 cut(s)	1063, 1833
EcoT38I	GRGCYC	3 cut(s)	439, 1531, 2101
ErhI	CCWWGG	3 cut(s)	902, 1263, 1778
FaqI	GGGAC	4 cut(s)	655, 680, 1181, 1946
FauI	CCCGC	1 cut(s)	643
FbaI	TGATCA	1 cut(s)	1162
FblI	GTMKAC	1 cut(s)	2017
Fnu4HI	GCNGC	9 cut(s)	197, 648, 1392, 1594, 1604, 1838, 2064, 2211, 2277
FokI	GGATG	4 cut(s)	430, 681, 1302, 1666
FriOI	GRGCYC	3 cut(s)	439, 1531, 2101
Fsp4HI	GCNGC	9 cut(s)	197, 648, 1392, 1594, 1604, 1838, 2064, 2211, 2277
FspBI	CTAG	6 cut(s)	440, 479, 1296, 1343, 2021, 2180
GlaI	GCGC	6 cut(s)	18, 362, 1468, 1586, 2292, 2332
GluI	GCNGC	9 cut(s)	197, 648, 1392, 1594, 1604, 1838, 2064, 2211, 2277
GsaI	CCCAGC	3 cut(s)	1024, 1032, 1967
GsuI	CTGGAG	4 cut(s)	197, 1026, 1854, 2225
HaeII	RGCGCY	2 cut(s)	2294, 2334
HaeIII	GGCC	7 cut(s)	907, 1359, 1400, 2025, 2246, 2279, 2372
HapII	CCGG	2 cut(s)	200, 386
HhaI	GCGC	6 cut(s)	19, 363, 1469, 1587, 2293, 2333
Hin6I	GCGC	6 cut(s)	17, 361, 1467, 1585, 2291, 2331
HinP1I	GCGC	6 cut(s)	17, 361, 1467, 1585, 2291, 2331
HindIII	AAGCTT	1 cut(s)	380
HpaII	CCGG	2 cut(s)	200, 386
HphI	GGTGA	2 cut(s)	569, 827
Hpy166II	GTNNAC	9 cut(s)	295, 796, 1553, 1758, 1877, 1879, 2018, 2323, 2416
Hpy8I	GTNNAC	9 cut(s)	295, 796, 1553, 1758, 1877, 1879, 2018, 2323, 2416
Hpy99I	CGWCG	3 cut(s)	139, 233, 498
HpyAV	CCTTC	9 cut(s)	350, 812, 839, 864, 946, 1349, 1715, 1982, 2025
HpyCH4III	ACNGT	7 cut(s)	103, 299, 884, 1312, 1388, 1900, 2327
HpyCH4IV	ACGT	6 cut(s)	66, 137, 180, 1303, 1957, 1993
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1025, 1121, 1397, 1593, 1837, 2096, 2273, 2299
HpyF3I	CTNAG	8 cut(s)	108, 399, 655, 858, 915, 1290, 1852, 2102
HpySE526I	ACGT	6 cut(s)	66, 137, 180, 1303, 1957, 1993
HspAI	GCGC	6 cut(s)	17, 361, 1467, 1585, 2291, 2331
Ksp22I	TGATCA	1 cut(s)	1162
Kzo9I	GATC	7 cut(s)	4, 706, 1162, 1249, 1506, 1774, 1848
LmnI	GCTCC	5 cut(s)	216, 1339, 1534, 1600, 2060
Lsp1109I	GCAGC	7 cut(s)	659, 1378, 1605, 1615, 1849, 2050, 2222
LweI	GCATC	8 cut(s)	173, 452, 457, 965, 1678, 1688, 1906, 2085
MaeI	CTAG	6 cut(s)	440, 479, 1296, 1343, 2021, 2180
MaeII	ACGT	6 cut(s)	66, 137, 180, 1303, 1957, 1993
MaeIII	GTNAC	3 cut(s)	497, 606, 2034
MalI	GATC	7 cut(s)	6, 708, 1164, 1251, 1508, 1776, 1850
MboI	GATC	7 cut(s)	4, 706, 1162, 1249, 1506, 1774, 1848
MboII	GAAGA	8 cut(s)	202, 242, 595, 627, 641, 879, 1259, 2334
MfeI	CAATTG	1 cut(s)	1543
MflI	RGATCY	1 cut(s)	1774
MhlI	GDGCHC	7 cut(s)	439, 1021, 1531, 1555, 2101, 2304, 2325
MlyI	GAGTC	1 cut(s)	1301
MmeI	TCCRAC	3 cut(s)	158, 1286, 2370
Mph1103I	ATGCAT	2 cut(s)	1063, 1833
MroXI	GAANNNNTTC	1 cut(s)	849
MseI	TTAA	3 cut(s)	1334, 1409, 1713
MslI	CAYNNNNRTG	3 cut(s)	1929, 2261, 2312
MspA1I	CMGCKG	2 cut(s)	650, 2213
MspI	CCGG	2 cut(s)	200, 386
MspR9I	CCNGG	7 cut(s)	201, 245, 387, 1320, 1396, 1872, 2028
MunI	CAATTG	1 cut(s)	1543
Mva1269I	GAATGC	1 cut(s)	720
MvaI	CCWGG	5 cut(s)	245, 1320, 1396, 1872, 2028
MwoI	GCNNNNNNNGC	8 cut(s)	1025, 1121, 1397, 1593, 1837, 2096, 2273, 2299
NciI	CCSGG	2 cut(s)	201, 387
NcoI	CCATGG	2 cut(s)	902, 1263
NdeII	GATC	7 cut(s)	4, 706, 1162, 1249, 1506, 1774, 1848
NheI	GCTAGC	1 cut(s)	1342
NlaIV	GGNNCC	8 cut(s)	279, 328, 840, 1530, 1668, 1776, 2254, 2280
NmeAIII	GCCGAG	1 cut(s)	2092
NmuCI	GTSAC	1 cut(s)	497
NsiI	ATGCAT	2 cut(s)	1063, 1833
NspI	RCATGY	2 cut(s)	1028, 1934
PaeI	GCATGC	2 cut(s)	1028, 1934
PceI	AGGCCT	1 cut(s)	1359
PcsI	WCGNNNNNNNCGW	1 cut(s)	134
PctI	GAATGC	1 cut(s)	720
PdmI	GAANNNNTTC	1 cut(s)	849
PfeI	GAWTC	9 cut(s)	130, 311, 632, 850, 1082, 1099, 1653, 1727, 2391
PkrI	GCNGC	9 cut(s)	198, 649, 1393, 1595, 1605, 1839, 2065, 2212, 2278
PleI	GAGTC	1 cut(s)	1300
PpsI	GAGTC	1 cut(s)	1300
Ppu21I	YACGTR	1 cut(s)	1958
PshAI	GACNNNNGTC	1 cut(s)	2352
PshBI	ATTAAT	1 cut(s)	1409
Psp124BI	GAGCTC	1 cut(s)	439
Psp1406I	AACGTT	1 cut(s)	66
Psp6I	CCWGG	5 cut(s)	243, 1318, 1394, 1870, 2026
PspFI	CCCAGC	3 cut(s)	1020, 1028, 1963
PspGI	CCWGG	5 cut(s)	243, 1318, 1394, 1870, 2026
PspN4I	GGNNCC	8 cut(s)	279, 328, 840, 1530, 1668, 1776, 2254, 2280
PspPI	GGNCC	6 cut(s)	1398, 2024, 2245, 2278, 2370, 2402
PsuI	RGATCY	1 cut(s)	1774
PvuII	CAGCTG	1 cut(s)	2213
RsaI	GTAC	5 cut(s)	307, 414, 1878, 1956, 2425
RsaNI	GTAC	5 cut(s)	306, 413, 1877, 1955, 2424
RseI	CAYNNNNRTG	3 cut(s)	1929, 2261, 2312
SacI	GAGCTC	1 cut(s)	439
SaqAI	TTAA	3 cut(s)	1334, 1409, 1713
SatI	GCNGC	9 cut(s)	197, 648, 1392, 1594, 1604, 1838, 2064, 2211, 2277
Sau3AI	GATC	7 cut(s)	4, 706, 1162, 1249, 1506, 1774, 1848
Sau96I	GGNCC	6 cut(s)	1398, 2024, 2245, 2278, 2370, 2402
SchI	GAGTC	1 cut(s)	1301
ScrFI	CCNGG	7 cut(s)	201, 245, 387, 1320, 1396, 1872, 2028
SduI	GDGCHC	7 cut(s)	439, 1021, 1531, 1555, 2101, 2304, 2325
SfaNI	GCATC	8 cut(s)	173, 452, 457, 965, 1678, 1688, 1906, 2085
SinI	GGWCC	1 cut(s)	2402
SmiMI	CAYNNNNRTG	3 cut(s)	1929, 2261, 2312
SmlI	CTYRAG	2 cut(s)	1125, 1631
SmoI	CTYRAG	2 cut(s)	1125, 1631
SphI	GCATGC	2 cut(s)	1028, 1934
SseBI	AGGCCT	1 cut(s)	1359
SsiI	CCGC	4 cut(s)	60, 197, 650, 2276
SspI	AATATT	1 cut(s)	1975
SspMI	CTAG	6 cut(s)	440, 479, 1296, 1343, 2021, 2180
SstI	GAGCTC	1 cut(s)	439
StuI	AGGCCT	1 cut(s)	1359
StyD4I	CCNGG	7 cut(s)	199, 243, 385, 1318, 1394, 1870, 2026
StyI	CCWWGG	3 cut(s)	902, 1263, 1778
TaaI	ACNGT	7 cut(s)	103, 299, 884, 1312, 1388, 1900, 2327
TaiI	ACGT	6 cut(s)	69, 140, 183, 1306, 1960, 1996
TaqII	GACCGA	2 cut(s)	464, 1635
TatI	WGTACW	1 cut(s)	1876
TauI	GCSGC	2 cut(s)	199, 2279
TfiI	GAWTC	9 cut(s)	130, 311, 632, 850, 1082, 1099, 1653, 1727, 2391
Tru1I	TTAA	3 cut(s)	1334, 1409, 1713
Tru9I	TTAA	3 cut(s)	1334, 1409, 1713
TscAI	CASTG	1 cut(s)	1393
TseFI	GTSAC	1 cut(s)	497
TseI	GCWGC	7 cut(s)	647, 1391, 1593, 1603, 1837, 2063, 2210
Tsp45I	GTSAC	1 cut(s)	497
TspDTI	ATGAA	7 cut(s)	650, 683, 879, 1231, 1304, 1719, 2424
TspGWI	ACGGA	5 cut(s)	1095, 1506, 1679, 1758, 2461
TspRI	CASTG	1 cut(s)	1393
VneI	GTGCAC	2 cut(s)	1551, 2321
VpaK11BI	GGWCC	1 cut(s)	2402
VspI	ATTAAT	1 cut(s)	1409
XagI	CCTNNNNNAGG	1 cut(s)	1107
XapI	RAATTY	4 cut(s)	1067, 1274, 1444, 1641
XceI	RCATGY	2 cut(s)	1028, 1934
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1459
XmiI	GTMKAC	1 cut(s)	2017
XmnI	GAANNNNTTC	1 cut(s)	849
XspI	CTAG	6 cut(s)	440, 479, 1296, 1343, 2021, 2180
Zsp2I	ATGCAT	2 cut(s)	1063, 1833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.