Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003103

Physical Location & Seq

Genomic Coordinates Forward (+)

25008071 .. 25021354

Gene Structure 13284 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1241000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3213 bp

ATGAGTCAATCGAGAGGCTCACGGGCGGATTTGGAGGCCGGCGGCAGCCGCTCGGGCGACTTGGACGACGAGTCCTCGTCCAGTCCTTTTGACATTGCCAGAACTAAGGATGCTTCGGTCGACCGCCTCAAGCGCTGGAGGCAAGCGGCACTTGTGCTGAATGCTTCTCGTCGATTCCGGTACACCTTAGACTTGAAAAAGGAAGAAGAAAAGCAGCAGACACTAAGGAAGATTAGAGCCCATGCACAAGCCATACGAGCTGCATATCTTTTCAAGGAAGCTGGAGACCAGCAAGTGAATGGAGTCGTAGTACCAAAACCAAGTTCTACTGGCGATTTTCCAATTGGACAAGAGGAACTAGTTCCAGTGACGAGGGATCATAATTTTGCTGCTCTTCAACAATATGGGGGGGTCACAGGCTTAGGGGACCTATTGAAAACAAATCTCGACAAGGGAATTCATGGAGATGATGTTGATTTACTAAAACGGAAAAATGCATTTGGGTCGAACACATATCCTAGGAAAAAAGCAAGAAGTTTCTGGAGATTTCTTTGGGAAGCTTGGCAAGATCTTACTCTGATCATTTTGATGGTAGCTGCAGTGGCTTCTTTAGCGCTGGGTATAAAAACAGAGGGTATAAAGGAGGGATGGTATGATGGGGGCAGCATTGCTTTTGCAGTTCTCCTTGTCATTGTTGTCACAGCCGTTAGTGATTACAGACAATCTCTTCAGTTCCAGAATTTAAATGAGGAAAAGAGAAATATACATTTGGAAGTCATTAGAGGTGGTAGACGTGTTGAGGTCTCAATTTATGATCTTGTTGTTGGTGATGTTGTACCCCTTAATATCGGCGATCAGGTCCCTGCTGACGGCATTTTAATAACTGGGTACTCACTTTCAATCGATGAGTCGAGTATGACTGGAGAGAGCAAGATTGTTCGCAAGGACTCCAAGGACCCATTTCTAATGTCTGGCTGCAAGGTAGCAGATGGAAATGGTACTATGCTGGTAACTAGTGTTGGGATTAATACTGAATGGGGAATGCTCATGGCTAGTATTTCTGAAGATACTGGTGAAGAAACTCCTCTGCAGGTGCGCTTGAACGGGGTTGCGACTTTCATTGGTATTGTCGGACTCACAGTGGCTTTTCTTGTCTTGATAGTCCTGTTGGTTAGATACTTTACTGGCCATACAAAAGATACAACTGGAAAACCTCAGTTTACCGCTGGAAAGACAAAATTTGGAGATGCCATAGATGGAGCCATTAAAATTGTTACTATTGCAGTCACCATTGTGGTGGTTGCTGTTCCTGAAGGGCTCCCTTTAGCTGTTACCTTGACGCTTGCCTACTCGATGAGAAAAATGATGGCAGATAAGGCCTTGGTGCGGAGGCTGTCTGCATGTGAAACCATGGGTTCTGCAACTACAATCTGCAGTGACAAGACTGGAACCTTAACATTAAACCAGATGACTGTTGTTGAGGCTTTTACGGGTGGGAGGAAAATCGTTCTCTCTGGCGATAAGCCAGACTTGTCTCCAATGCTATCATCTTTGCTTATTGAAGGCATCGCATTCAATACAAATGGCAGTGTTTATATGCCTGAGACCGGCGGTGATATAGAGGTTTCTGGATCACCAACTGAAAAGGCAATTCTGCAATTGGGAATCAAGCTTGGAATGAATTTTGATGTCATAAGGTCTGAGTCTTCACTTCTTCATGTGTTCCCATTCAACTCTGAGAAAAAAAGAGGCGGTGCTGCGGTTAAATTGCCCAATTCCGAAGTTCATATACACTGGAAAGGGGCTGCTGAAATAGTCTTGGCCTGTTGTACAAAGTACCTGGATCCAAATGACAAGTTGGTAGCTATGGATGATGAGAAGTCAAAAATTTTCAGGAAATCAATTGAGGATATGGCTGCTCGCAGTTTGCGTTGTGTTGCCATTGCTTATAGACCACATGAATTGGAAGATGTTCCAACTGATGGACAACTATCTGAGTGGGCATTGCCTGATGATGACCTTGTCTTGCTTGCTATTGTTGGTATAAAGGATCCTTGTCGGCCTGGGGTGGGGGATGCAGTGCGACTATGCCAAAAAGCTGGTGTTAAGGTGCGCATGGTCACGGGTGACAATGTTCAAACTGCGAAAGCAATTGCTATGGAATGTGGCATACTTGCTCCAGATTCAGATGCTACCGAGCCAACTCTTATTGAAGGAAAAGTTTTCCGTGAACTTTCGGATAAGCAGAAAGAAGATTATGCTGAAAAGATTTCGGTGATGGGGCGGTCTTCCCCCAATGACAAGCTTCTGCTTGTGCAAGCATTGAGGAGAAGGGGTCATGTTGTTGCTGTAACCGGAGATGGCACTAATGATGCTCCTGCACTGCATGAGGCAGACATTGGTCTTGCAATGGGTATTCAAGGGACTGAAGTTGCCAAAGAGAGTTCAGATATCATTATCTTGGACGATAATTTTGCTTCAGTTGTGAAGGTTGTTAGATGGGGTCGATCTGTATATGCAAATATTCAGAAATTCATCCAATTCCAGCTTACAGTCAATGTTGCAGCACTAATTATAAATGTCGTGGCAGCCATTTCATCTGGTGACGTTCCACTAAATGCTGTTCAGCTTCTGTGGGTGAATCTTATCATGGATACACTTGGAGCTCTAGCATTGGCTACGGAGCCACCTACAGATCACTTGATGGACAGAACCCCTGTTGGTCGAAGAGAGCCGCTCATAACAAATATAATGTGGAGGAACTTGTTGGTTCAGGCTTTCTACCAAGTCACTGTCCTACTTGTCCTCACCTTCCGAGGAATAAGTATACTGAAGCTGCATGGTCCCAATGCTACCACAGTGAAGAATACTCTGATATTCAATGCATTTGTCCTATGTCAAATTTTCAATGAATTCAATGCTCGAAAGCCCGATGAGTTCAATATATTCCAAGGGATAACCAAGAACTTCCTTTTTATGGGAATAGTAGCAGTAACTCTCGTACTTCAGATTATCATCGTCGAGTTCCTTGGGAAGTTCACTAAAACAAAACCCCTTACTTGGGATCTTTGGCTGATTTCATCTGTAATAGCTTTTATCAGTTGGCCTCTCGCTGTTGTTGGGAAACTGATTCCGGTTCCAGAAACTCCGTTATACAAGTATTTCACAAGAATGATCCCTAGGCGAAGAAACAATCCAGAGGTTAGTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1071

Amino Acids

117.02

Weight (kDa)

6.92

Isoelectric Point (pI)

37.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	28 - 70	3.4e-17	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	136 - 203	4.3e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	257 - 352	3.7e-21	P-type ATPase actuator domain
Hydrolase	PF00702	476 - 799	3.2e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	519 - 688	9.1e-31	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	870 - 1043	2.4e-42	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29900 AT4G29900 AT5G57110 AT5G57110 AT5G57110
fragaria_vesca	FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940
malus_domestica	MD13G1241000.v1.1 MD16G1245600.v1.1 MD16G1246100.v1.1
prunus_persica	Prupe.1G072600_v2.0.a1 Prupe.8G012900_v2.0.a1
pyrus_communis	pycom13g21220
rosa_chinensis	RchiOBHm_Chr4g0398891 RchiOBHm_Chr4g0398951
rosa_laevigata	RLG00000009307 RLG00000009309 RLG00000009365
rosa_multiflora	Rmu_sc0004094.1_g000033 Rmu_ssc0000064.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343340 Rroxscaffold_5G00343930 Rroxscaffold_5G00344000
rosa_rugosa	Rorug04G0013200
rosa_samantha	Rh4AG091100 Rh4CG093100 Rh4CG099100 Rh4CG099400
rosa_wichuraiana	Rw4G007100 Rw4G007130 Rw4G007500 Rw4G007540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2576
AarI	CACCTGC	1 cut(s)	1081
Acc16I	TGCGCA	1 cut(s)	2114
Acc36I	ACCTGC	1 cut(s)	1081
AccB7I	CCANNNNNTGG	1 cut(s)	1982
AccBSI	CCGCTC	2 cut(s)	51, 2737
AccI	GTMKAC	3 cut(s)	120, 790, 2827
AclWI	GGATC	8 cut(s)	384, 1639, 1838, 1851, 2045, 2058, 3072, 3169
AcoI	YGGCCR	1 cut(s)	1186
AcsI	RAATTY	8 cut(s)	456, 739, 1238, 1681, 1887, 2531, 2901, 2912
AcuI	CTGAAG	7 cut(s)	713, 1083, 1332, 2448, 2463, 2852, 2990
AfaI	GTAC	8 cut(s)	182, 312, 837, 890, 1000, 1831, 1838, 3003
AfeI	AGCGCT	2 cut(s)	134, 615
AfiI	CCNNNNNNNGG	8 cut(s)	869, 1386, 1607, 1982, 2069, 2977, 3060, 3061
AflIII	ACRYGT	1 cut(s)	793
AhdI	GACNNNNNGTC	1 cut(s)	70
AhlI	ACTAGT	2 cut(s)	358, 1013
AjiI	CACGTC	1 cut(s)	794
AjnI	CCWGG	2 cut(s)	1839, 2062
AjuI	GAANNNNNNNTTGG	2 cut(s)	307, 339
AleI	CACNNNNGTG	1 cut(s)	1292
Alw21I	GWGCWC	1 cut(s)	2668
Alw26I	GTCTC	4 cut(s)	279, 808, 1539, 1598
AlwI	GGATC	8 cut(s)	384, 1639, 1838, 1851, 2045, 2058, 3072, 3169
AlwNI	CAGNNNCTG	1 cut(s)	2632
Ama87I	CYCGRG	1 cut(s)	52
Aor51HI	AGCGCT	2 cut(s)	134, 615
AoxI	GGCC	6 cut(s)	36, 1186, 1377, 1821, 2060, 3104
ApoI	RAATTY	8 cut(s)	456, 739, 1238, 1681, 1887, 2531, 2901, 2912
ArsI	GACNNNNNNTTYG	2 cut(s)	2456, 2488
AseI	ATTAAT	1 cut(s)	1026
Asp700I	GAANNNNTTC	2 cut(s)	360, 1971
AspA2I	CCTAGG	2 cut(s)	518, 3179
AspLEI	GCGC	4 cut(s)	135, 616, 1098, 2115
AspS9I	GGNCC	4 cut(s)	427, 859, 955, 2843
AvaI	CYCGRG	1 cut(s)	52
AvaII	GGWCC	4 cut(s)	427, 859, 955, 2843
AvrII	CCTAGG	2 cut(s)	518, 3179
BalI	TGGCCA	1 cut(s)	1188
BamHI	GGATCC	2 cut(s)	1843, 2050
BanII	GRGCYC	3 cut(s)	241, 1320, 2668
BarI	GAAGNNNNNNTAC	2 cut(s)	1773, 1805
BbsI	GAAGAC	2 cut(s)	1698, 2280
Bbv12I	GWGCWC	1 cut(s)	2668
BceAI	ACGGC	2 cut(s)	689, 886
BcgI	CGANNNNNNTGC	4 cut(s)	486, 520, 2456, 2490
BciT130I	CCWGG	2 cut(s)	1841, 2064
BciVI	GTATCC	1 cut(s)	2647
BclI	TGATCA	1 cut(s)	579
BcoDI	GTCTC	4 cut(s)	279, 808, 1539, 1598
BcuI	ACTAGT	2 cut(s)	358, 1013
BfaI	CTAG	6 cut(s)	359, 519, 1014, 1053, 2669, 3180
BfmI	CTRYAG	4 cut(s)	597, 1088, 1432, 2691
BfoI	RGCGCY	2 cut(s)	136, 617
BfuAI	ACCTGC	1 cut(s)	1081
BfuI	GTATCC	1 cut(s)	2647
BglI	GCCNNNNNGGC	1 cut(s)	54
BglII	AGATCT	1 cut(s)	568
BlnI	CCTAGG	2 cut(s)	518, 3179
Bme1390I	CCNGG	2 cut(s)	1841, 2064
Bme18I	GGWCC	4 cut(s)	427, 859, 955, 2843
BmeRI	GACNNNNNGTC	1 cut(s)	70
BmeT110I	CYCGRG	1 cut(s)	52
BmgBI	CACGTC	1 cut(s)	794
BmgT120I	GGNCC	4 cut(s)	427, 859, 955, 2843
BmrFI	CCNGG	2 cut(s)	1841, 2064
BmrI	ACTGGG	1 cut(s)	894
BmsI	GCATC	6 cut(s)	100, 1237, 1575, 2065, 2179, 2362
BmuI	ACTGGG	1 cut(s)	894
BoxI	GACNNNNGTC	1 cut(s)	2400
BpiI	GAAGAC	2 cut(s)	1698, 2280
BplI	GAGNNNNNCTC	2 cut(s)	2721, 2753
BpmI	CTGGAG	5 cut(s)	157, 303, 562, 942, 2163
Bpu10I	CCTNAGC	1 cut(s)	421
BpuEI	CTTGAG	1 cut(s)	113
Bsa29I	ATCGAT	1 cut(s)	903
BsaBI	GATNNNNATC	1 cut(s)	3014
BsaI	GGTCTC	3 cut(s)	279, 808, 1598
BsaJI	CCNNGG	9 cut(s)	518, 951, 1380, 1410, 2063, 2815, 2950, 3028, 3179
BsaWI	WCCGGW	3 cut(s)	177, 2354, 3133
BsaXI	ACNNNNNCTCC	8 cut(s)	294, 324, 456, 486, 635, 665, 2320, 2350
Bsc4I	CCNNNNNNNGG	8 cut(s)	869, 1386, 1607, 1982, 2069, 2977, 3060, 3061
Bse118I	RCCGGY	2 cut(s)	38, 1607
Bse3DI	GCAATG	5 cut(s)	93, 666, 1941, 2003, 2415
Bse8I	GATNNNNATC	1 cut(s)	3014
BseBI	CCWGG	2 cut(s)	1841, 2064
BseCI	ATCGAT	1 cut(s)	903
BseDI	CCNNGG	9 cut(s)	518, 951, 1380, 1410, 2063, 2815, 2950, 3028, 3179
BseGI	GGATG	5 cut(s)	115, 653, 1876, 2080, 2535
BseJI	GATNNNNATC	1 cut(s)	3014
BseLI	CCNNNNNNNGG	8 cut(s)	869, 1386, 1607, 1982, 2069, 2977, 3060, 3061
BseMI	GCAATG	5 cut(s)	93, 666, 1941, 2003, 2415
BseMII	CTCAG	5 cut(s)	1229, 1593, 1692, 1728, 1986
BseRI	GAGGAG	2 cut(s)	1074, 2341
BseYI	CCCAGC	1 cut(s)	616
BsgI	GTGCAG	1 cut(s)	2364
Bsh1285I	CGRYCG	2 cut(s)	120, 124
BshFI	GGCC	6 cut(s)	38, 1188, 1379, 1823, 2062, 3106
BshVI	ATCGAT	1 cut(s)	903
BsiEI	CGRYCG	2 cut(s)	120, 124
BsiHKAI	GWGCWC	1 cut(s)	2668
BsiHKCI	CYCGRG	1 cut(s)	52
BsiSI	CCGG	5 cut(s)	39, 178, 1608, 2355, 3134
BslFI	GGGAC	4 cut(s)	440, 845, 2437, 2829
BslI	CCNNNNNNNGG	8 cut(s)	869, 1386, 1607, 1982, 2069, 2977, 3060, 3061
BsmAI	GTCTC	4 cut(s)	279, 808, 1539, 1598
BsmFI	GGGAC	4 cut(s)	440, 845, 2437, 2829
BsmI	GAATGC	3 cut(s)	166, 1047, 1571
BsnI	GGCC	6 cut(s)	38, 1188, 1379, 1823, 2062, 3106
Bso31I	GGTCTC	3 cut(s)	279, 808, 1598
BsoBI	CYCGRG	1 cut(s)	52
Bsp1286I	GDGCHC	3 cut(s)	241, 1320, 2668
Bsp1407I	TGTACA	1 cut(s)	1829
Bsp19I	CCATGG	1 cut(s)	1410
BspANI	GGCC	6 cut(s)	38, 1188, 1379, 1823, 2062, 3106
BspCNI	CTCAG	5 cut(s)	1228, 1594, 1693, 1729, 1987
BspDI	ATCGAT	1 cut(s)	903
BspMAI	CTGCAG	3 cut(s)	601, 1092, 1436
BspMI	ACCTGC	1 cut(s)	1081
BspPI	GGATC	8 cut(s)	384, 1639, 1838, 1851, 2045, 2058, 3072, 3169
BspQI	GCTCTTC	1 cut(s)	399
BspTNI	GGTCTC	3 cut(s)	279, 808, 1598
BsrBI	CCGCTC	2 cut(s)	51, 2737
BsrDI	GCAATG	5 cut(s)	93, 666, 1941, 2003, 2415
BsrFI	RCCGGY	2 cut(s)	38, 1607
BsrGI	TGTACA	1 cut(s)	1829
BssAI	RCCGGY	2 cut(s)	38, 1607
BssECI	CCNNGG	9 cut(s)	518, 951, 1380, 1410, 2063, 2815, 2950, 3028, 3179
BssNAI	GTATAC	1 cut(s)	2828
BssT1I	CCWWGG	7 cut(s)	518, 951, 1380, 1410, 2950, 3028, 3179
Bst1107I	GTATAC	1 cut(s)	2828
Bst2UI	CCWGG	2 cut(s)	1841, 2064
Bst4CI	ACNGT	5 cut(s)	1141, 1474, 2554, 2794, 2860
Bst6I	CTCTTC	3 cut(s)	399, 732, 2722
BstAUI	TGTACA	1 cut(s)	1829
BstC8I	GCNNGC	6 cut(s)	40, 144, 1344, 1921, 2031, 2319
BstDEI	CTNAG	9 cut(s)	105, 187, 224, 421, 1215, 1602, 1701, 1737, 1995
BstDSI	CCRYGG	1 cut(s)	1410
BstF5I	GGATG	5 cut(s)	115, 653, 1876, 2080, 2535
BstH2I	RGCGCY	2 cut(s)	136, 617
BstHHI	GCGC	4 cut(s)	135, 616, 1098, 2115
BstMAI	GTCTC	4 cut(s)	279, 808, 1539, 1598
BstMCI	CGRYCG	2 cut(s)	120, 124
BstMWI	GCNNNNNNNGC	8 cut(s)	48, 54, 132, 139, 257, 602, 611, 1376
BstNI	CCWGG	2 cut(s)	1841, 2064
BstNSI	RCATGY	1 cut(s)	1404
BstPAI	GACNNNNGTC	1 cut(s)	2400
BstSCI	CCNGG	2 cut(s)	1839, 2062
BstSFI	CTRYAG	4 cut(s)	597, 1088, 1432, 2691
BstV2I	GAAGAC	2 cut(s)	1698, 2280
BstX2I	RGATCY	4 cut(s)	568, 1843, 2050, 3064
BstXI	CCANNNNNNTGG	2 cut(s)	1297, 2099
BstYI	RGATCY	4 cut(s)	568, 1843, 2050, 3064
BstZ17I	GTATAC	1 cut(s)	2828
Bsu15I	ATCGAT	1 cut(s)	903
BsuI	GTATCC	1 cut(s)	2647
BsuRI	GGCC	6 cut(s)	38, 1188, 1379, 1823, 2062, 3106
BsuTUI	ATCGAT	1 cut(s)	903
BtgI	CCRYGG	1 cut(s)	1410
BtgZI	GCGATG	1 cut(s)	1552
BtrI	CACGTC	1 cut(s)	794
BtsCI	GGATG	5 cut(s)	115, 653, 1876, 2080, 2535
BtsI	GCAGTG	5 cut(s)	606, 1441, 1594, 2085, 2381
BveI	ACCTGC	1 cut(s)	1081
Cac8I	GCNNGC	6 cut(s)	40, 144, 1344, 1921, 2031, 2319
CaiI	CAGNNNCTG	1 cut(s)	2632
CfoI	GCGC	4 cut(s)	135, 616, 1098, 2115
Cfr10I	RCCGGY	2 cut(s)	38, 1607
Cfr13I	GGNCC	4 cut(s)	427, 859, 955, 2843
ClaI	ATCGAT	1 cut(s)	903
CseI	GACGC	1 cut(s)	1348
Csp6I	GTAC	8 cut(s)	181, 311, 836, 889, 999, 1830, 1837, 3002
CspCI	CAANNNNNGTGG	2 cut(s)	1944, 1979
CviQI	GTAC	8 cut(s)	181, 311, 836, 889, 999, 1830, 1837, 3002
DdeI	CTNAG	9 cut(s)	105, 187, 224, 421, 1215, 1602, 1701, 1737, 1995
DraI	TTTAAA	1 cut(s)	744
DriI	GACNNNNNGTC	1 cut(s)	70
EaeI	YGGCCR	1 cut(s)	1186
Eam1104I	CTCTTC	3 cut(s)	399, 732, 2722
Eam1105I	GACNNNNNGTC	1 cut(s)	70
EarI	CTCTTC	3 cut(s)	399, 732, 2722
EciI	GGCGGA	1 cut(s)	41
Ecl136II	GAGCTC	1 cut(s)	2666
Eco130I	CCWWGG	7 cut(s)	518, 951, 1380, 1410, 2950, 3028, 3179
Eco147I	AGGCCT	1 cut(s)	1379
Eco24I	GRGCYC	3 cut(s)	241, 1320, 2668
Eco31I	GGTCTC	3 cut(s)	279, 808, 1598
Eco32I	GATATC	1 cut(s)	2452
Eco47I	GGWCC	4 cut(s)	427, 859, 955, 2843
Eco47III	AGCGCT	2 cut(s)	134, 615
Eco53kI	GAGCTC	1 cut(s)	2666
Eco57I	CTGAAG	7 cut(s)	713, 1083, 1332, 2448, 2463, 2852, 2990
Eco88I	CYCGRG	1 cut(s)	52
EcoICRI	GAGCTC	1 cut(s)	2666
EcoO109I	RGGNCCY	3 cut(s)	427, 859, 955
EcoRI	GAATTC	2 cut(s)	456, 2912
EcoRII	CCWGG	2 cut(s)	1839, 2062
EcoRV	GATATC	1 cut(s)	2452
EcoT14I	CCWWGG	7 cut(s)	518, 951, 1380, 1410, 2950, 3028, 3179
EcoT22I	ATGCAT	2 cut(s)	499, 2887
EcoT38I	GRGCYC	3 cut(s)	241, 1320, 2668
ErhI	CCWWGG	7 cut(s)	518, 951, 1380, 1410, 2950, 3028, 3179
FalI	AAGNNNNNCTT	4 cut(s)	135, 167, 1514, 1546
FaqI	GGGAC	4 cut(s)	440, 845, 2437, 2829
FbaI	TGATCA	1 cut(s)	579
FblI	GTMKAC	3 cut(s)	120, 790, 2827
FokI	GGATG	5 cut(s)	122, 660, 1883, 2087, 2522
FriOI	GRGCYC	3 cut(s)	241, 1320, 2668
FspAI	RTGCGCAY	1 cut(s)	2114
FspBI	CTAG	6 cut(s)	359, 519, 1014, 1053, 2669, 3180
FspI	TGCGCA	1 cut(s)	2114
GlaI	GCGC	4 cut(s)	134, 615, 1097, 2114
GsaI	CCCAGC	1 cut(s)	620
GsuI	CTGGAG	5 cut(s)	157, 303, 562, 942, 2163
HaeII	RGCGCY	2 cut(s)	136, 617
HaeIII	GGCC	6 cut(s)	38, 1188, 1379, 1823, 2062, 3106
HapII	CCGG	5 cut(s)	39, 178, 1608, 2355, 3134
HgaI	GACGC	1 cut(s)	1348
HhaI	GCGC	4 cut(s)	135, 616, 1098, 2115
Hin6I	GCGC	4 cut(s)	133, 614, 1096, 2113
HinP1I	GCGC	4 cut(s)	133, 614, 1096, 2113
HincII	GTYRAC	1 cut(s)	121
HindII	GTYRAC	1 cut(s)	121
HindIII	AAGCTT	3 cut(s)	558, 1670, 2303
HpaII	CCGG	5 cut(s)	39, 178, 1608, 2355, 3134
Hpy166II	GTNNAC	7 cut(s)	121, 183, 791, 1221, 2231, 2828, 3039
Hpy8I	GTNNAC	7 cut(s)	121, 183, 791, 1221, 2231, 2828, 3039
Hpy99I	CGWCG	3 cut(s)	71, 174, 3023
HpyAV	CCTTC	6 cut(s)	1307, 1556, 2207, 2325, 2482, 2821
HpyCH4III	ACNGT	5 cut(s)	1141, 1474, 2554, 2794, 2860
HpyCH4IV	ACGT	2 cut(s)	793, 2607
HpyF10VI	GCNNNNNNNGC	8 cut(s)	48, 54, 132, 139, 257, 602, 611, 1376
HpyF3I	CTNAG	9 cut(s)	105, 187, 224, 421, 1215, 1602, 1701, 1737, 1995
HpySE526I	ACGT	2 cut(s)	793, 2607
HspAI	GCGC	4 cut(s)	133, 614, 1096, 2113
KroI	GCCGGC	1 cut(s)	38
KroNI	GCCGGC	1 cut(s)	40
Ksp22I	TGATCA	1 cut(s)	579
LguI	GCTCTTC	1 cut(s)	399
LmnI	GCTCC	6 cut(s)	1259, 1323, 2182, 2380, 2663, 2683
LweI	GCATC	6 cut(s)	100, 1237, 1575, 2065, 2179, 2362
MaeI	CTAG	6 cut(s)	359, 519, 1014, 1053, 2669, 3180
MaeII	ACGT	2 cut(s)	793, 2607
MbiI	CCGCTC	2 cut(s)	51, 2737
MfeI	CAATTG	4 cut(s)	342, 1658, 1902, 2151
MflI	RGATCY	4 cut(s)	568, 1843, 2050, 3064
MhlI	GDGCHC	3 cut(s)	241, 1320, 2668
MlsI	TGGCCA	1 cut(s)	1188
MluNI	TGGCCA	1 cut(s)	1188
MlyI	GAGTC	7 cut(s)	13, 80, 312, 917, 941, 1128, 1712
MmeI	TCCRAC	2 cut(s)	1111, 2000
Mox20I	TGGCCA	1 cut(s)	1188
Mph1103I	ATGCAT	2 cut(s)	499, 2887
MroNI	GCCGGC	1 cut(s)	38
MroXI	GAANNNNTTC	2 cut(s)	360, 1971
MscI	TGGCCA	1 cut(s)	1188
MseI	TTAA	9 cut(s)	743, 843, 878, 1026, 1266, 1454, 1460, 1764, 2106
MslI	CAYNNNNRTG	5 cut(s)	465, 587, 1292, 1295, 3170
Msp20I	TGGCCA	1 cut(s)	1188
MspA1I	CMGCKG	1 cut(s)	1226
MspI	CCGG	5 cut(s)	39, 178, 1608, 2355, 3134
MspR9I	CCNGG	2 cut(s)	1841, 2064
MunI	CAATTG	4 cut(s)	342, 1658, 1902, 2151
Mva1269I	GAATGC	3 cut(s)	166, 1047, 1571
MvaI	CCWGG	2 cut(s)	1841, 2064
MwoI	GCNNNNNNNGC	8 cut(s)	48, 54, 132, 139, 257, 602, 611, 1376
NaeI	GCCGGC	1 cut(s)	40
NcoI	CCATGG	1 cut(s)	1410
NgoMIV	GCCGGC	1 cut(s)	38
NmuCI	GTSAC	9 cut(s)	367, 412, 697, 1285, 1436, 2119, 2126, 2603, 2788
NsbI	TGCGCA	1 cut(s)	2114
NsiI	ATGCAT	2 cut(s)	499, 2887
NspI	RCATGY	1 cut(s)	1404
OliI	CACNNNNGTG	1 cut(s)	1292
PaqCI	CACCTGC	1 cut(s)	1081
PceI	AGGCCT	1 cut(s)	1379
PciSI	GCTCTTC	1 cut(s)	399
PcsI	WCGNNNNNNNCGW	1 cut(s)	1927
PctI	GAATGC	3 cut(s)	166, 1047, 1571
PdiI	GCCGGC	1 cut(s)	40
PdmI	GAANNNNTTC	2 cut(s)	360, 1971
PfeI	GAWTC	5 cut(s)	174, 1665, 2183, 2641, 3130
PflFI	GACNNNGTC	1 cut(s)	2021
PflMI	CCANNNNNTGG	1 cut(s)	1982
PleI	GAGTC	7 cut(s)	12, 79, 311, 916, 941, 1128, 1711
PpsI	GAGTC	7 cut(s)	12, 79, 311, 916, 941, 1128, 1711
PpuMI	RGGWCCY	3 cut(s)	427, 859, 955
PshAI	GACNNNNGTC	1 cut(s)	2400
PshBI	ATTAAT	1 cut(s)	1026
PsiI	TTATAA	1 cut(s)	2576
Psp124BI	GAGCTC	1 cut(s)	2668
Psp5II	RGGWCCY	3 cut(s)	427, 859, 955
Psp6I	CCWGG	2 cut(s)	1839, 2062
PspFI	CCCAGC	1 cut(s)	616
PspGI	CCWGG	2 cut(s)	1839, 2062
PspPI	GGNCC	4 cut(s)	427, 859, 955, 2843
PspPPI	RGGWCCY	3 cut(s)	427, 859, 955
PstI	CTGCAG	3 cut(s)	601, 1092, 1436
PstNI	CAGNNNCTG	1 cut(s)	2632
PsuI	RGATCY	4 cut(s)	568, 1843, 2050, 3064
PsyI	GACNNNGTC	1 cut(s)	2021
RsaI	GTAC	8 cut(s)	182, 312, 837, 890, 1000, 1831, 1838, 3003
RsaNI	GTAC	8 cut(s)	181, 311, 836, 889, 999, 1830, 1837, 3002
RseI	CAYNNNNRTG	5 cut(s)	465, 587, 1292, 1295, 3170
SacI	GAGCTC	1 cut(s)	2668
SalI	GTCGAC	1 cut(s)	119
SapI	GCTCTTC	1 cut(s)	399
SaqAI	TTAA	9 cut(s)	743, 843, 878, 1026, 1266, 1454, 1460, 1764, 2106
Sau96I	GGNCC	4 cut(s)	427, 859, 955, 2843
SchI	GAGTC	7 cut(s)	13, 80, 312, 917, 941, 1128, 1712
ScrFI	CCNGG	2 cut(s)	1841, 2064
SduI	GDGCHC	3 cut(s)	241, 1320, 2668
SfaNI	GCATC	6 cut(s)	100, 1237, 1575, 2065, 2179, 2362
SfcI	CTRYAG	4 cut(s)	597, 1088, 1432, 2691
SinI	GGWCC	4 cut(s)	427, 859, 955, 2843
SmiI	ATTTAAAT	1 cut(s)	744
SmiMI	CAYNNNNRTG	5 cut(s)	465, 587, 1292, 1295, 3170
SmlI	CTYRAG	1 cut(s)	128
SmoI	CTYRAG	1 cut(s)	128
SpeI	ACTAGT	2 cut(s)	358, 1013
SseBI	AGGCCT	1 cut(s)	1379
SspI	AATATT	1 cut(s)	2524
SspMI	CTAG	6 cut(s)	359, 519, 1014, 1053, 2669, 3180
SstI	GAGCTC	1 cut(s)	2668
StuI	AGGCCT	1 cut(s)	1379
StyD4I	CCNGG	2 cut(s)	1839, 2062
StyI	CCWWGG	7 cut(s)	518, 951, 1380, 1410, 2950, 3028, 3179
SwaI	ATTTAAAT	1 cut(s)	744
TaaI	ACNGT	5 cut(s)	1141, 1474, 2554, 2794, 2860
TaiI	ACGT	2 cut(s)	796, 2610
TaqII	GACCGA	1 cut(s)	106
TatI	WGTACW	1 cut(s)	1829
TauI	GCSGC	4 cut(s)	45, 51, 149, 2737
TfiI	GAWTC	5 cut(s)	174, 1665, 2183, 2641, 3130
Tru1I	TTAA	9 cut(s)	743, 843, 878, 1026, 1266, 1454, 1460, 1764, 2106
Tru9I	TTAA	9 cut(s)	743, 843, 878, 1026, 1266, 1454, 1460, 1764, 2106
TseFI	GTSAC	9 cut(s)	367, 412, 697, 1285, 1436, 2119, 2126, 2603, 2788
Tsp45I	GTSAC	9 cut(s)	367, 412, 697, 1285, 1436, 2119, 2126, 2603, 2788
TspGWI	ACGGA	4 cut(s)	502, 2216, 2696, 3138
Tth111I	GACNNNGTC	1 cut(s)	2021
Van91I	CCANNNNNTGG	1 cut(s)	1982
VpaK11BI	GGWCC	4 cut(s)	427, 859, 955, 2843
VspI	ATTAAT	1 cut(s)	1026
XapI	RAATTY	8 cut(s)	456, 739, 1238, 1681, 1887, 2531, 2901, 2912
XceI	RCATGY	1 cut(s)	1404
XcmI	CCANNNNNNNNNTGG	1 cut(s)	296
XmaJI	CCTAGG	2 cut(s)	518, 3179
XmiI	GTMKAC	3 cut(s)	120, 790, 2827
XmnI	GAANNNNTTC	2 cut(s)	360, 1971
XspI	CTAG	6 cut(s)	359, 519, 1014, 1053, 2669, 3180
Zsp2I	ATGCAT	2 cut(s)	499, 2887

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.