Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003103

Physical Location & Seq

Genomic Coordinates Forward (+)

50752742 .. 50764152

Gene Structure 11411 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009309

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3252 bp

ATGAGTCAATCAACGGGCTCGCCGAACCAGAAAAACTATGATGAGGAAGCCGGAATCAACCGTCCCGGCGACGACGAGGACTGCTTGAATGTTTTTGATATTACCAGAACTAAGAATGCTTCCGTCGAGCGTCTCCGCCGTTGGAGGCAAGCGGCACTTGTGCTAAATGCTTCTCGTCGATTCCGGTATACCTTAGACTTGAAAAAGGAGGAAGAAAAAAAGAAGACGTTAAGCAAGATTAGAGTACATGCGCAAGCCATACGAGCTGCATTTCTTTTCAAGGACTCCTTAAGACTGGAAAATGGAATTGTGCCGCCAAAACCAGGTCCTGCTGGTGAATTTGCAATTGGACAAGAAGAACTAGCTTCAATTTCAAGGGATCATAATTTTACTACTCTGCAGCAATATGGGGGGGTCAAAGGCCTTGGAGAATTATTGAAAACAAGCTTAGAAAAGGGAATTCCTGGAAGTGATGATGATTTACTAAAACGAAAAACTGCATATGGATCCAACACATATCCCCGGAAAAAACCAAGAAGTTTCTGGAGATTTCTCTGGGAAGCTTGCCAAGATCTTACTCTTATCATTTTGATGGTAGCTGCTGTGGCGTCTTTAGCACTGGGTATAAAAACAGAGGGTATAAAGGAGGGATGGTATGATGGGGGCAGCATTGCTTTTGCAGTTCTCCTAGTCATTGTCGTGACAGCCATTAGTGACTACAAACAATCTCTGCAGTTCCAGAATCTAAATGAGGAAAAGAGAAATATACAAATTGAGGTTGTCAGAGGTGGTAGACGTGTTGAGGTTTCAATTTATGAACTTGTTGTAGGTGATGTTATACCCCTTAACATTGGTGATCAGGTTCCTGCTGATGGGGTTTTAATCACTGGTCATTCACTTTCAATTGATGAGTCTAGCATGACTGGCGAGTCCAAGATTGTTCGCAAGGACTCCAAAGAACCATTTCTAATGTCCGGCTGCAAAGTAGCAGATGGAAATGGTATAATGCTGGTAACCAGTGTTGGGATTAATACTGAATGGGGATTACTCATGGCTAGCATCTCGGAAGACACTGGTGAAGAAACACCCTTGCAGGTGCGCTTGAATGGGGTTGCTACCTTCATTGGTATTGTGGGGCTCACAGTAGCTTTCCTTGTCTTATGTGTCCTTTTAGTCAGATACTTTACTGGCCATACAACAAATGCAGATGGAACTCCTCAGTTTGTTGCCGGAAAGACAAAATTTGGGAAAGCCATAGATGGAGCCATCAAAATTGTCACTATTGCAGTCACCATTGTAGTGGTTGCTGTTCCTGAAGGCCTCCCCTTAGCTGTTACCTTAACACTTGCGTACTCAATGAGAAAAATGATGGCAGATAAGGCCTTGGTACGTAGGCTTTCTGCATGTGAAACAATGGGCTCGGCCACTACTATATGCAGTGATAAGACTGGTACCTTAACTTTGAACCAGATGACTGTGGTCGAGTCTTGTGCATGTTTGAAGAAAGTCAATAACTCTGATGGCAAGCCAGCCTTGTCGCCAAAAATATCGTCTTTGATTATTGAAGGCATAGCTCAGAATACAACTGGCAATGTTTATGTGCCCGAGACTGGTGGTGATGAAGAGGTTACTGGATCACCAACGGAGAAGGCAATTCTGCAGTGGGCACTCAAGCTTGGGATGAATTTTGAAGCCACCAGGTCTCAATCTTCAATTCTTCATGTGTTCCCATTCAACTCTGAGAAAAAACGAGGCGGTGTAGCAGTTAAATTGCCAAACAATGAAGTTCATATACACTGGAAAGGGGCTGCTGAAATAATATTGGCCTCATGCAGAAGATACATTGACGACAATGATCAGGTGGCTGCAATGGAAGACGACAAGCTTATGTTTTTCAGGAAATCTATTGAGGATATGGCTGGTCGTAGTTTGCGTTGTGTTGCTATAGCATATTTACCATATGAATTGGAAAATGTTCCAACTGGTGAACAACAATTAGCTGATTGGGCAATGCCTGCGGATGACCTTGTTTTACTTGCTATTGTTGGTATCAAGGATCCTTGTCGACCAGGAGTCGCAGATGCAGTGAGACTATGCCAAAAGGCTGGCGTCAAGGTACGCATGGTTACTGGAGACAATGTTCAGACTGCAAAGGCAATAGCATTAGAATGTGGTATACTGCGTACTGATTCAGATCTTAGTGAGCATCTAATTGAAGGGAAAGACTTCCGTGAGCTTTCTGATAGGCTGAGAGAAGAGGTTGCTGAGAAAATCTCGGTAATGGGACGGTCCTCTCCTAATGATAAGCTTCTGCTTGTGCAAGCCCTAAGGAGAAGGGGACATGTTGTTGCTGTTACTGGAGATGGCACTAATGATGCTCCTGCACTACATGAGGCCGACATTGGTCTTGCTATGGGTATTCAAGGTACTGAGGTTGCCAAAGAGAGTTCTGATATCATTATCTTGGATGACAATTTTGCTTCAGTTGTGAAGGTTGTCAGATGGGGACGCTCTGTATATGCAAATATTCAGAAATTCATCCAATTCCAACTCACTGTTAATGTTGCTGCACTTGTTATCAATGTTGTGGCAGCGATTTCCTCTGGTGAAGTACCACTAAATGCTGTTCAGCTTCTGTGGGTGAATCTTATAATGGATACTCTTGGAGCACTAGCATTGGCTACTGAGCCTCCTACGAATCATCTGATGGACAGACCTCCTGTTGGTCGAAGGGAACCACTGATAACTAATATAATGTGGAGGAACTTATTGATTCAGGCTATCTACCAAATTACGGTCCTCCTTATCTTCAATTTCCGAGGGAAAAGTATTCTCAATCTGGAACATGATTCTACTGACCATGCGGACAAAGTGAAGAACACACTTATATTTAATACCTTTGTCCTTTGTCAAATTTTCAATGAATTCAATGCCAGGAAGCCGGATGAATTCAACATATTCAAAGGAATTACCAAAAATTATCTCTTTATGGGCATAATTGCAGTGACACTTGTACTTCAGATTCTCATTGTCGAGTTTCTGGGGAAGTTTACTACAACAGTCCGGCTTAACTGGAAGCATTGGTTGATTTCTGTTGTCATCGCTTTTATCAGTTGGCCTCTTGCTGTTGTTGGGAAGTTTATTCCGGTACCCAAAACTCCCTTCCACAGATATTTCACTAGGAGTCCTCTCTTTAAGTTTATAACCCGAATATTCCGTCGGCGAAGAGAGAGAACAGAGGCCTGTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1084

Amino Acids

119.47

Weight (kDa)

8.49

Isoelectric Point (pI)

38.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	31 - 72	1.4e-17	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	136 - 204	4.4e-14	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	258 - 353	9.5e-21	P-type ATPase actuator domain
Hydrolase	PF00702	478 - 800	8.3e-15	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	519 - 690	3.3e-30	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	871 - 1047	6e-45	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0003103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29900 AT4G29900 AT5G57110 AT5G57110 AT5G57110
fragaria_vesca	FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940
malus_domestica	MD13G1241000.v1.1 MD16G1245600.v1.1 MD16G1246100.v1.1
prunus_persica	Prupe.1G072600_v2.0.a1 Prupe.8G012900_v2.0.a1
pyrus_communis	pycom13g21220
rosa_chinensis	RchiOBHm_Chr4g0398891 RchiOBHm_Chr4g0398951
rosa_laevigata	RLG00000009307 RLG00000009309 RLG00000009365
rosa_multiflora	Rmu_sc0004094.1_g000033 Rmu_ssc0000064.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343340 Rroxscaffold_5G00343930 Rroxscaffold_5G00344000
rosa_rugosa	Rorug04G0013200
rosa_samantha	Rh4AG091100 Rh4CG093100 Rh4CG099100 Rh4CG099400
rosa_wichuraiana	Rw4G007100 Rw4G007130 Rw4G007500 Rw4G007540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2651, 3203
AarI	CACCTGC	1 cut(s)	1084
AasI	GACNNNNNNGTC	1 cut(s)	928
Acc16I	TGCGCA	1 cut(s)	252
Acc36I	ACCTGC	1 cut(s)	1084
Acc65I	GGTACC	2 cut(s)	1451, 3148
AccB1I	GGYRCC	2 cut(s)	1451, 3148
AccI	GTMKAC	4 cut(s)	188, 793, 2065, 2176
AciI	CCGC	6 cut(s)	136, 152, 314, 1755, 2018, 2864
AclWI	GGATC	6 cut(s)	387, 501, 514, 1642, 2051, 2064
AcoI	YGGCCR	2 cut(s)	1189, 1422
AcsI	RAATTY	8 cut(s)	338, 459, 1241, 1684, 2534, 2913, 2924, 2948
AcuI	CTGAAG	3 cut(s)	1335, 2466, 3002
AcyI	GRCGYC	2 cut(s)	608, 2109
AfiI	CCNNNNNNNGG	6 cut(s)	323, 872, 1023, 1610, 2723, 2794
AflII	CTTAAG	1 cut(s)	289
AflIII	ACRYGT	2 cut(s)	796, 2341
AhdI	GACNNNNNGTC	1 cut(s)	2072
AjiI	CACGTC	1 cut(s)	797
AjnI	CCWGG	5 cut(s)	322, 463, 1697, 2068, 2933
AjuI	GAANNNNNNNTTGG	4 cut(s)	330, 362, 3146, 3178
AloI	GAACNNNNNNTCC	2 cut(s)	2124, 2156
Alw21I	GWGCWC	1 cut(s)	2671
Alw26I	GTCTC	5 cut(s)	137, 1601, 1707, 2083, 2127
AlwI	GGATC	6 cut(s)	387, 501, 514, 1642, 2051, 2064
AlwNI	CAGNNNCTG	2 cut(s)	329, 2635
Ama87I	CYCGRG	1 cut(s)	1604
AoxI	GGCC	9 cut(s)	421, 1189, 1318, 1380, 1422, 1824, 2394, 3116, 3240
ApeKI	GCWGC	9 cut(s)	266, 400, 599, 666, 978, 1808, 1865, 2567, 2591
ApoI	RAATTY	8 cut(s)	338, 459, 1241, 1684, 2534, 2913, 2924, 2948
ArsI	GACNNNNNNTTYG	4 cut(s)	1535, 1567, 3202, 3234
AseI	ATTAAT	1 cut(s)	1029
Asp700I	GAANNNNTTC	3 cut(s)	963, 1974, 2225
Asp718I	GGTACC	2 cut(s)	1451, 3148
AspLEI	GCGC	2 cut(s)	253, 1101
AspS9I	GGNCC	3 cut(s)	326, 2289, 2797
AsuC2I	CCSGG	2 cut(s)	66, 523
AsuNHI	GCTAGC	1 cut(s)	1055
AvaI	CYCGRG	1 cut(s)	1604
AvaII	GGWCC	3 cut(s)	326, 2289, 2797
AxyI	CCTNAGG	1 cut(s)	2327
BaeGI	GKGCMC	2 cut(s)	1605, 1669
BaeI	ACNNNNGTAYC	2 cut(s)	2418, 2451
BalI	TGGCCA	1 cut(s)	1191
BamHI	GGATCC	2 cut(s)	506, 2056
BanI	GGYRCC	2 cut(s)	1451, 3148
BanII	GRGCYC	3 cut(s)	20, 1140, 1421
BarI	GAAGNNNNNNTAC	2 cut(s)	1776, 1808
BbsI	GAAGAC	3 cut(s)	230, 1074, 1881
Bbv12I	GWGCWC	1 cut(s)	2671
BbvI	GCAGC	9 cut(s)	253, 412, 586, 678, 965, 1795, 1852, 2554, 2603
BceAI	ACGGC	1 cut(s)	123
BcgI	CGANNNNNNTGC	2 cut(s)	1472, 1506
BciT130I	CCWGG	5 cut(s)	324, 465, 1699, 2070, 2935
BciVI	GTATCC	1 cut(s)	2650
BclI	TGATCA	2 cut(s)	856, 1855
BcnI	CCSGG	2 cut(s)	66, 523
BcoDI	GTCTC	5 cut(s)	137, 1601, 1707, 2083, 2127
BfaI	CTAG	6 cut(s)	362, 689, 915, 1056, 2672, 3180
BfmI	CTRYAG	4 cut(s)	398, 731, 1658, 1944
BfrI	CTTAAG	1 cut(s)	289
BfuAI	ACCTGC	1 cut(s)	1084
BfuI	GTATCC	1 cut(s)	2650
BglII	AGATCT	2 cut(s)	571, 2194
Bme1390I	CCNGG	7 cut(s)	66, 324, 465, 523, 1699, 2070, 2935
Bme18I	GGWCC	3 cut(s)	326, 2289, 2797
BmeRI	GACNNNNNGTC	1 cut(s)	2072
BmeT110I	CYCGRG	1 cut(s)	1604
BmgBI	CACGTC	1 cut(s)	797
BmgT120I	GGNCC	3 cut(s)	326, 2289, 2797
BmiI	GGNNCC	7 cut(s)	508, 864, 1264, 1453, 2058, 2736, 3150
BmrFI	CCNGG	7 cut(s)	66, 324, 465, 523, 1699, 2070, 2935
BmrI	ACTGGG	1 cut(s)	629
BmsI	GCATC	4 cut(s)	1068, 2071, 2215, 2365
BmtI	GCTAGC	1 cut(s)	1059
BmuI	ACTGGG	1 cut(s)	629
BoxI	GACNNNNGTC	2 cut(s)	1478, 2403
BpiI	GAAGAC	3 cut(s)	230, 1074, 1881
BplI	GAGNNNNNCTC	2 cut(s)	2258, 2290
BpmI	CTGGAG	3 cut(s)	565, 2151, 2379
Bpu10I	CCTNAGC	1 cut(s)	1327
BpuEI	CTTGAG	1 cut(s)	1655
BpuMI	CCSGG	2 cut(s)	66, 523
BsaAI	YACGTR	1 cut(s)	1391
BsaBI	GATNNNNATC	1 cut(s)	2193
BsaHI	GRCGYC	2 cut(s)	608, 2109
BsaI	GGTCTC	1 cut(s)	1707
BsaJI	CCNNGG	4 cut(s)	424, 521, 1383, 2818
BsaWI	WCCGGW	2 cut(s)	183, 3145
BsaXI	ACNNNNNCTCC	6 cut(s)	638, 668, 2124, 2154, 2674, 2704
Bsc4I	CCNNNNNNNGG	6 cut(s)	323, 872, 1023, 1610, 2723, 2794
Bse21I	CCTNAGG	1 cut(s)	2327
Bse3DI	GCAATG	4 cut(s)	669, 1597, 1875, 2016
Bse8I	GATNNNNATC	1 cut(s)	2193
BseBI	CCWGG	5 cut(s)	324, 465, 1699, 2070, 2935
BseDI	CCNNGG	4 cut(s)	424, 521, 1383, 2818
BseGI	GGATG	6 cut(s)	656, 1686, 2026, 2473, 2538, 2950
BseJI	GATNNNNATC	1 cut(s)	2193
BseLI	CCNNNNNNNGG	6 cut(s)	323, 872, 1023, 1610, 2723, 2794
BseMI	GCAATG	4 cut(s)	669, 1597, 1875, 2016
BseMII	CTCAG	7 cut(s)	1232, 1589, 1731, 2240, 2256, 2421, 2676
BseRI	GAGGAG	1 cut(s)	1206
BseSI	GKGCMC	2 cut(s)	1605, 1669
BseXI	GCAGC	9 cut(s)	253, 412, 586, 678, 965, 1795, 1852, 2554, 2603
BsgI	GTGCAG	2 cut(s)	2367, 2553
BshFI	GGCC	9 cut(s)	423, 1191, 1320, 1382, 1424, 1826, 2396, 3118, 3242
BshNI	GGYRCC	2 cut(s)	1451, 3148
BsiHKAI	GWGCWC	1 cut(s)	2671
BsiHKCI	CYCGRG	1 cut(s)	1604
BsiSI	CCGG	9 cut(s)	51, 66, 184, 523, 975, 1230, 2942, 3064, 3146
BslFI	GGGAC	4 cut(s)	48, 2298, 2352, 2520
BslI	CCNNNNNNNGG	6 cut(s)	323, 872, 1023, 1610, 2723, 2794
BsmAI	GTCTC	5 cut(s)	137, 1601, 1707, 2083, 2127
BsmBI	CGTCTC	1 cut(s)	137
BsmFI	GGGAC	4 cut(s)	48, 2298, 2352, 2520
BsmI	GAATGC	1 cut(s)	121
BsnI	GGCC	9 cut(s)	423, 1191, 1320, 1382, 1424, 1826, 2396, 3118, 3242
Bso31I	GGTCTC	1 cut(s)	1707
BsoBI	CYCGRG	1 cut(s)	1604
Bsp1286I	GDGCHC	6 cut(s)	20, 1140, 1421, 1605, 1669, 2671
Bsp143I	GATC	8 cut(s)	379, 506, 571, 856, 1634, 1855, 2056, 2194
BspACI	CCGC	6 cut(s)	136, 152, 314, 1755, 2018, 2864
BspANI	GGCC	9 cut(s)	423, 1191, 1320, 1382, 1424, 1826, 2396, 3118, 3242
BspCNI	CTCAG	7 cut(s)	1231, 1588, 1732, 2241, 2257, 2422, 2677
BspLI	GGNNCC	7 cut(s)	508, 864, 1264, 1453, 2058, 2736, 3150
BspMAI	CTGCAG	3 cut(s)	402, 735, 1662
BspMI	ACCTGC	1 cut(s)	1084
BspOI	GCTAGC	1 cut(s)	1059
BspPI	GGATC	6 cut(s)	387, 501, 514, 1642, 2051, 2064
BspT107I	GGYRCC	2 cut(s)	1451, 3148
BspTI	CTTAAG	1 cut(s)	289
BspTNI	GGTCTC	1 cut(s)	1707
BsrDI	GCAATG	4 cut(s)	669, 1597, 1875, 2016
BssECI	CCNNGG	4 cut(s)	424, 521, 1383, 2818
BssMI	GATC	8 cut(s)	379, 506, 571, 856, 1634, 1855, 2056, 2194
BssNAI	GTATAC	2 cut(s)	189, 2177
BssNI	GRCGYC	2 cut(s)	608, 2109
BssT1I	CCWWGG	2 cut(s)	424, 1383
Bst1107I	GTATAC	2 cut(s)	189, 2177
Bst2UI	CCWGG	5 cut(s)	324, 465, 1699, 2070, 2935
Bst4CI	ACNGT	7 cut(s)	62, 1144, 1477, 2289, 2557, 2797, 3061
Bst6I	CTCTTC	3 cut(s)	1617, 2250, 3220
BstACI	GRCGYC	2 cut(s)	608, 2109
BstAFI	CTTAAG	1 cut(s)	289
BstAPI	GCANNNNNTGC	1 cut(s)	2015
BstBAI	YACGTR	1 cut(s)	1391
BstEII	GGTNACC	1 cut(s)	1012
BstF5I	GGATG	6 cut(s)	656, 1686, 2026, 2473, 2538, 2950
BstHHI	GCGC	2 cut(s)	253, 1101
BstKTI	GATC	8 cut(s)	382, 509, 574, 859, 1637, 1858, 2059, 2197
BstMAI	GTCTC	5 cut(s)	137, 1601, 1707, 2083, 2127
BstMBI	GATC	8 cut(s)	379, 506, 571, 856, 1634, 1855, 2056, 2194
BstMWI	GCNNNNNNNGC	7 cut(s)	263, 605, 614, 924, 1379, 2006, 2015
BstNI	CCWGG	5 cut(s)	324, 465, 1699, 2070, 2935
BstNSI	RCATGY	4 cut(s)	251, 1407, 1497, 2345
BstPAI	GACNNNNGTC	2 cut(s)	1478, 2403
BstPI	GGTNACC	1 cut(s)	1012
BstSCI	CCNGG	7 cut(s)	64, 322, 463, 521, 1697, 2068, 2933
BstSFI	CTRYAG	4 cut(s)	398, 731, 1658, 1944
BstSLI	GKGCMC	2 cut(s)	1605, 1669
BstSNI	TACGTA	1 cut(s)	1391
BstV1I	GCAGC	9 cut(s)	253, 412, 586, 678, 965, 1795, 1852, 2554, 2603
BstV2I	GAAGAC	3 cut(s)	230, 1074, 1881
BstX2I	RGATCY	4 cut(s)	506, 571, 2056, 2194
BstXI	CCANNNNNNTGG	2 cut(s)	1300, 2105
BstYI	RGATCY	4 cut(s)	506, 571, 2056, 2194
BstZ17I	GTATAC	2 cut(s)	189, 2177
Bsu36I	CCTNAGG	1 cut(s)	2327
BsuI	GTATCC	1 cut(s)	2650
BsuRI	GGCC	9 cut(s)	423, 1191, 1320, 1382, 1424, 1826, 2396, 3118, 3242
BtgZI	GCGATG	1 cut(s)	3085
BtrI	CACGTC	1 cut(s)	797
BtsCI	GGATG	6 cut(s)	656, 1686, 2026, 2473, 2538, 2950
BtsI	GCAGTG	4 cut(s)	1444, 1667, 2091, 3009
BveI	ACCTGC	1 cut(s)	1084
CaiI	CAGNNNCTG	2 cut(s)	329, 2635
CfoI	GCGC	2 cut(s)	253, 1101
Cfr13I	GGNCC	3 cut(s)	326, 2289, 2797
CseI	GACGC	4 cut(s)	119, 597, 2098, 2517
CsiI	ACCWGGT	2 cut(s)	322, 1697
DpnI	GATC	8 cut(s)	381, 508, 573, 858, 1636, 1857, 2058, 2196
DpnII	GATC	8 cut(s)	379, 506, 571, 856, 1634, 1855, 2056, 2194
DrdI	GACNNNNNNGTC	1 cut(s)	928
DriI	GACNNNNNGTC	1 cut(s)	2072
DseDI	GACNNNNNNGTC	1 cut(s)	928
EaeI	YGGCCR	2 cut(s)	1189, 1422
Eam1104I	CTCTTC	3 cut(s)	1617, 2250, 3220
Eam1105I	GACNNNNNGTC	1 cut(s)	2072
EarI	CTCTTC	3 cut(s)	1617, 2250, 3220
EciI	GGCGGA	1 cut(s)	125
Eco105I	TACGTA	1 cut(s)	1391
Eco130I	CCWWGG	2 cut(s)	424, 1383
Eco147I	AGGCCT	4 cut(s)	423, 1320, 1382, 3242
Eco24I	GRGCYC	3 cut(s)	20, 1140, 1421
Eco31I	GGTCTC	1 cut(s)	1707
Eco32I	GATATC	1 cut(s)	2455
Eco47I	GGWCC	3 cut(s)	326, 2289, 2797
Eco57I	CTGAAG	3 cut(s)	1335, 2466, 3002
Eco81I	CCTNAGG	1 cut(s)	2327
Eco88I	CYCGRG	1 cut(s)	1604
Eco91I	GGTNACC	1 cut(s)	1012
EcoO109I	RGGNCCY	1 cut(s)	326
EcoO65I	GGTNACC	1 cut(s)	1012
EcoRI	GAATTC	3 cut(s)	459, 2924, 2948
EcoRII	CCWGG	5 cut(s)	322, 463, 1697, 2068, 2933
EcoRV	GATATC	1 cut(s)	2455
EcoT14I	CCWWGG	2 cut(s)	424, 1383
EcoT38I	GRGCYC	3 cut(s)	20, 1140, 1421
ErhI	CCWWGG	2 cut(s)	424, 1383
Esp3I	CGTCTC	1 cut(s)	137
FalI	AAGNNNNNCTT	6 cut(s)	141, 173, 272, 304, 1517, 1549
FaqI	GGGAC	4 cut(s)	48, 2298, 2352, 2520
FauNDI	CATATG	2 cut(s)	502, 1960
FbaI	TGATCA	2 cut(s)	856, 1855
FblI	GTMKAC	4 cut(s)	188, 793, 2065, 2176
FokI	GGATG	6 cut(s)	663, 1693, 2033, 2480, 2525, 2957
FriOI	GRGCYC	3 cut(s)	20, 1140, 1421
FspBI	CTAG	6 cut(s)	362, 689, 915, 1056, 2672, 3180
FspI	TGCGCA	1 cut(s)	252
GlaI	GCGC	2 cut(s)	252, 1100
GsuI	CTGGAG	3 cut(s)	565, 2151, 2379
HaeIII	GGCC	9 cut(s)	423, 1191, 1320, 1382, 1424, 1826, 2396, 3118, 3242
HapII	CCGG	9 cut(s)	51, 66, 184, 523, 975, 1230, 2942, 3064, 3146
HgaI	GACGC	4 cut(s)	119, 597, 2098, 2517
HhaI	GCGC	2 cut(s)	253, 1101
Hin1I	GRCGYC	2 cut(s)	608, 2109
Hin6I	GCGC	2 cut(s)	251, 1099
HinP1I	GCGC	2 cut(s)	251, 1099
HincII	GTYRAC	1 cut(s)	2066
HindII	GTYRAC	1 cut(s)	2066
HindIII	AAGCTT	5 cut(s)	445, 561, 1673, 1883, 2306
HpaII	CCGG	9 cut(s)	51, 66, 184, 523, 975, 1230, 2942, 3064, 3146
Hpy166II	GTNNAC	6 cut(s)	189, 794, 1988, 2066, 2177, 3051
Hpy188III	TCNNGA	6 cut(s)	544, 700, 739, 1313, 1897, 2840
Hpy8I	GTNNAC	6 cut(s)	189, 794, 1988, 2066, 2177, 3051
Hpy99I	CGWCG	5 cut(s)	74, 77, 128, 180, 3222
HpyAV	CCTTC	9 cut(s)	1129, 1310, 1559, 1642, 2210, 2328, 2485, 2724, 3172
HpyCH4III	ACNGT	7 cut(s)	62, 1144, 1477, 2289, 2557, 2797, 3061
HpyCH4IV	ACGT	3 cut(s)	227, 796, 1390
HpyF10VI	GCNNNNNNNGC	7 cut(s)	263, 605, 614, 924, 1379, 2006, 2015
HpySE526I	ACGT	3 cut(s)	227, 796, 1390
Hsp92I	GRCGYC	2 cut(s)	608, 2109
HspAI	GCGC	2 cut(s)	251, 1099
KpnI	GGTACC	2 cut(s)	1455, 3152
Ksp22I	TGATCA	2 cut(s)	856, 1855
Kzo9I	GATC	8 cut(s)	379, 506, 571, 856, 1634, 1855, 2056, 2194
LmnI	GCTCC	3 cut(s)	1262, 2383, 2666
Lsp1109I	GCAGC	9 cut(s)	253, 412, 586, 678, 965, 1795, 1852, 2554, 2603
LweI	GCATC	4 cut(s)	1068, 2071, 2215, 2365
MabI	ACCWGGT	2 cut(s)	322, 1697
MaeI	CTAG	6 cut(s)	362, 689, 915, 1056, 2672, 3180
MaeII	ACGT	3 cut(s)	227, 796, 1390
MalI	GATC	8 cut(s)	381, 508, 573, 858, 1636, 1857, 2058, 2196
MboI	GATC	8 cut(s)	379, 506, 571, 856, 1634, 1855, 2056, 2194
MfeI	CAATTG	2 cut(s)	345, 903
MflI	RGATCY	4 cut(s)	506, 571, 2056, 2194
MhlI	GDGCHC	6 cut(s)	20, 1140, 1421, 1605, 1669, 2671
MlsI	TGGCCA	1 cut(s)	1191
MluNI	TGGCCA	1 cut(s)	1191
MlyI	GAGTC	8 cut(s)	13, 278, 920, 938, 944, 1493, 2082, 3193
MmeI	TCCRAC	4 cut(s)	122, 534, 2003, 2572
Mox20I	TGGCCA	1 cut(s)	1191
MroXI	GAANNNNTTC	3 cut(s)	963, 1974, 2225
MscI	TGGCCA	1 cut(s)	1191
MslI	CAYNNNNRTG	4 cut(s)	590, 698, 1298, 2167
Msp20I	TGGCCA	1 cut(s)	1191
MspCI	CTTAAG	1 cut(s)	289
MspI	CCGG	9 cut(s)	51, 66, 184, 523, 975, 1230, 2942, 3064, 3146
MspR9I	CCNGG	7 cut(s)	66, 324, 465, 523, 1699, 2070, 2935
MunI	CAATTG	2 cut(s)	345, 903
Mva1269I	GAATGC	1 cut(s)	121
MvaI	CCWGG	5 cut(s)	324, 465, 1699, 2070, 2935
MwoI	GCNNNNNNNGC	7 cut(s)	263, 605, 614, 924, 1379, 2006, 2015
NciI	CCSGG	2 cut(s)	66, 523
NdeI	CATATG	2 cut(s)	502, 1960
NdeII	GATC	8 cut(s)	379, 506, 571, 856, 1634, 1855, 2056, 2194
NheI	GCTAGC	1 cut(s)	1055
NlaIV	GGNNCC	7 cut(s)	508, 864, 1264, 1453, 2058, 2736, 3150
NmeAIII	GCCGAG	1 cut(s)	1400
NmuCI	GTSAC	5 cut(s)	700, 713, 1276, 1288, 3004
NsbI	TGCGCA	1 cut(s)	252
NspI	RCATGY	4 cut(s)	251, 1407, 1497, 2345
PaqCI	CACCTGC	1 cut(s)	1084
PceI	AGGCCT	4 cut(s)	423, 1320, 1382, 3242
PciI	ACATGT	1 cut(s)	2341
PcsI	WCGNNNNNNNCGW	2 cut(s)	20, 1930
PctI	GAATGC	1 cut(s)	121
PdmI	GAANNNNTTC	3 cut(s)	963, 1974, 2225
PfeI	GAWTC	9 cut(s)	54, 180, 742, 2189, 2644, 2698, 2773, 2849, 3022
PfoI	TCCNGGA	1 cut(s)	463
PleI	GAGTC	8 cut(s)	12, 278, 919, 937, 944, 1492, 2081, 3192
PpsI	GAGTC	8 cut(s)	12, 278, 919, 937, 944, 1492, 2081, 3192
Ppu21I	YACGTR	1 cut(s)	1391
PpuMI	RGGWCCY	1 cut(s)	326
PscI	ACATGT	1 cut(s)	2341
PshAI	GACNNNNGTC	2 cut(s)	1478, 2403
PshBI	ATTAAT	1 cut(s)	1029
PsiI	TTATAA	2 cut(s)	2651, 3203
Psp5II	RGGWCCY	1 cut(s)	326
Psp6I	CCWGG	5 cut(s)	322, 463, 1697, 2068, 2933
PspEI	GGTNACC	1 cut(s)	1012
PspGI	CCWGG	5 cut(s)	322, 463, 1697, 2068, 2933
PspN4I	GGNNCC	7 cut(s)	508, 864, 1264, 1453, 2058, 2736, 3150
PspPI	GGNCC	3 cut(s)	326, 2289, 2797
PspPPI	RGGWCCY	1 cut(s)	326
PstI	CTGCAG	3 cut(s)	402, 735, 1662
PstNI	CAGNNNCTG	2 cut(s)	329, 2635
PsuI	RGATCY	4 cut(s)	506, 571, 2056, 2194
RseI	CAYNNNNRTG	4 cut(s)	590, 698, 1298, 2167
SalI	GTCGAC	1 cut(s)	2064
Sau3AI	GATC	8 cut(s)	379, 506, 571, 856, 1634, 1855, 2056, 2194
Sau96I	GGNCC	3 cut(s)	326, 2289, 2797
SchI	GAGTC	8 cut(s)	13, 278, 920, 938, 944, 1493, 2082, 3193
ScrFI	CCNGG	7 cut(s)	66, 324, 465, 523, 1699, 2070, 2935
SduI	GDGCHC	6 cut(s)	20, 1140, 1421, 1605, 1669, 2671
SexAI	ACCWGGT	2 cut(s)	322, 1697
SfaNI	GCATC	4 cut(s)	1068, 2071, 2215, 2365
SfcI	CTRYAG	4 cut(s)	398, 731, 1658, 1944
SinI	GGWCC	3 cut(s)	326, 2289, 2797
SmiMI	CAYNNNNRTG	4 cut(s)	590, 698, 1298, 2167
SmlI	CTYRAG	2 cut(s)	289, 1670
SmoI	CTYRAG	2 cut(s)	289, 1670
SnaBI	TACGTA	1 cut(s)	1391
SseBI	AGGCCT	4 cut(s)	423, 1320, 1382, 3242
SsiI	CCGC	6 cut(s)	136, 152, 314, 1755, 2018, 2864
SspI	AATATT	3 cut(s)	1821, 2527, 3213
SspMI	CTAG	6 cut(s)	362, 689, 915, 1056, 2672, 3180
StuI	AGGCCT	4 cut(s)	423, 1320, 1382, 3242
StyD4I	CCNGG	7 cut(s)	64, 322, 463, 521, 1697, 2068, 2933
StyI	CCWWGG	2 cut(s)	424, 1383
TaaI	ACNGT	7 cut(s)	62, 1144, 1477, 2289, 2557, 2797, 3061
TaiI	ACGT	3 cut(s)	230, 799, 1393
TaqI	TCGA	6 cut(s)	126, 178, 1482, 2065, 2728, 3033
TatI	WGTACW	2 cut(s)	244, 3013
TauI	GCSGC	2 cut(s)	155, 316
TfiI	GAWTC	9 cut(s)	54, 180, 742, 2189, 2644, 2698, 2773, 2849, 3022
TseFI	GTSAC	5 cut(s)	700, 713, 1276, 1288, 3004
TseI	GCWGC	9 cut(s)	266, 400, 599, 666, 978, 1808, 1865, 2567, 2591
Tsp45I	GTSAC	5 cut(s)	700, 713, 1276, 1288, 3004
TspGWI	ACGGA	4 cut(s)	112, 1658, 2219, 3206
Vha464I	CTTAAG	1 cut(s)	289
VpaK11BI	GGWCC	3 cut(s)	326, 2289, 2797
VspI	ATTAAT	1 cut(s)	1029
XapI	RAATTY	8 cut(s)	338, 459, 1241, 1684, 2534, 2913, 2924, 2948
XceI	RCATGY	4 cut(s)	251, 1407, 1497, 2345
XcmI	CCANNNNNNNNNTGG	1 cut(s)	540
XmiI	GTMKAC	4 cut(s)	188, 793, 2065, 2176
XmnI	GAANNNNTTC	3 cut(s)	963, 1974, 2225
XspI	CTAG	6 cut(s)	362, 689, 915, 1056, 2672, 3180

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.