Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0003103

Physical Location & Seq

Genomic Coordinates Forward (+)

16881648 .. 16908357

Gene Structure 26710 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG093100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGACTGTTGTTGAGGCTTATGTTGGTGGGAAGAAAATCTATCCTTCAGACAATAAATCGCGATTGTCTCCAGTGCTATGGTCTTTAATTATTGAAAGCATTGCACAGAACACAAATGGCAGTGTCTACATGTCTGAGAATGGTGGTGATGTAGAGGTTTATGGATCACCAACTGAAAAAGCAATTCTGCGCTGGGGGATTAAGATTGGAATGAATTTTGAAGCCACGAAGTCTGAATCTACAGTTCTTCATGCGCTCGCATTTAACTCGGTGAAGAAACGAAGTGCTGTTGCACTTAAATTGGCCAATTCTCAAGTTCATATACACTGGAAAGGGGCTGCTGAAACAATCTTGGCCTCATGCACAAAGTACATTGATGCTAATGATCACTTGGTAGCAATGGATGAAGATAAGTTAATGTTTTTCAATGAAGCTATTGAGGGTATGGCTTCTGGAAGTCTGCGGTGTGTTGCTATTGCATATAGGTTATACGAGTTTGAAAATGTTCCGACTGATGAACAACTAGTTCGGTGGGCATTACCTGAGGATGACCTTATTTTACTTGCTATTGTTGGCATAGAGGATCCTTGTCAGCCAGGAGTCAGAGATGCAGTTCGACAATGCCAAAATGCTGGTGTTAAGGTATGCATGGTTACGGGTGACAATCTTCAAACAGCTAAAGCAATTGCTTTGGAGTGTGGCATACTTGGTTCCGAAGCAGATGCTACTGAACAAAATATCATTGAAGGAAGAGTATTTCGTGCCTTTTCTGATAAGCAAAGAGAAGATTTAGCTGAGATGATATCAGTGATGGGACGATCTTCGCCCAATGACAAGCTTCTTCTTGTGCAAGCACTGAAGAAAAGGGGACATGTTGTTGCTGTCACTGGTGATGGTTGTAATGATGCTCCTGCATTATATGAGGCCGATATTGGTCTTGCTATGGGTATTCAAGGGGCTGAAGTTGCTAAAGAGAGTTCAGATATAATCATCTTGGATGATAACTTTTCTACAATTGTGAAGGCTATCAAATGGGGTCGATCTGTATATTCAAATATTCAGAAATTTACCCAGTTCCATCTTACAGTTGGACTTGCAGCATTAATCATAAATGTCATGGCAGAAGTTTCCTCTGGTTCAGCTCCATTAAATGCTGTGCAGATTCTCTGGGTAAGCATTATCATGGACACTCAAGGAGCACTAGTATTGCGTACTGATCCACCATCTGATAACCTGATGCGGAAGCCTCCTGTTGATCGAAGGGAACCTCTTATTACAAATATAGTGTGGAGGAACTTGTTGGTTCAGTCTTTCTACCAAGTCACTATCTTGCTTATTCTAAATTTCCACGGAAGGAATTTGCTGAATTTGGAACTTGCTGATTACAATGTGATGAATACAATGATATTCAATGCTTTTGTTCTTTGTCAAATTTTCAATGAATTCAATGCTCGAAAGCCTGAGGAATATAACATTTTCAAAGGAATTACTTCAAACTATCTCTTTGTGGGAACAGTGGGAATAACTCTTGTGCTTCAGATTATTGTCATCGAGTTCCTTGGGAAATTTACTTCAACTGTGAGGCTTAACTTGAAGCAGTGGCTGATTTCCGTCATCATTGGTTTTATCAGTTGGCCCCTTGATGCTATCGGGAAACTCATACCAGTTCCAAAAACTCCCTTCATCAAGTTTGCCTTGAAGCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

568

Amino Acids

62.75

Weight (kDa)

5.8

Isoelectric Point (pI)

40.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase	PF13246	39 - 199	2.8e-28	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	186 - 309	2.2e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	381 - 554	3.8e-42	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29900 AT4G29900 AT5G57110 AT5G57110 AT5G57110
fragaria_vesca	FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940
malus_domestica	MD13G1241000.v1.1 MD16G1245600.v1.1 MD16G1246100.v1.1
prunus_persica	Prupe.1G072600_v2.0.a1 Prupe.8G012900_v2.0.a1
pyrus_communis	pycom13g21220
rosa_chinensis	RchiOBHm_Chr4g0398891 RchiOBHm_Chr4g0398951
rosa_laevigata	RLG00000009307 RLG00000009309 RLG00000009365
rosa_multiflora	Rmu_sc0004094.1_g000033 Rmu_ssc0000064.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343340 Rroxscaffold_5G00343930 Rroxscaffold_5G00344000
rosa_rugosa	Rorug04G0013200
rosa_samantha	Rh4AG091100 Rh4CG093100 Rh4CG099100 Rh4CG099400
rosa_wichuraiana	Rw4G007100 Rw4G007130 Rw4G007500 Rw4G007540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	126
AccII	CGCG	1 cut(s)	61
AciI	CCGC	2 cut(s)	463, 1240
AclWI	GGATC	4 cut(s)	172, 578, 591, 1211
AcoI	YGGCCR	1 cut(s)	303
AcsI	RAATTY	8 cut(s)	214, 1064, 1342, 1357, 1366, 1431, 1442, 1565
AcuI	CTGAAG	4 cut(s)	30, 878, 981, 1520
AfaI	GTAC	2 cut(s)	371, 1213
AflIII	ACRYGT	2 cut(s)	129, 871
AhlI	ACTAGT	2 cut(s)	523, 1201
AjnI	CCWGG	1 cut(s)	595
AluBI	AGCT	5 cut(s)	434, 677, 794, 838, 1142
AluI	AGCT	5 cut(s)	434, 677, 794, 838, 1142
Alw21I	GWGCWC	1 cut(s)	1201
Alw26I	GTCTC	1 cut(s)	72
AlwI	GGATC	4 cut(s)	172, 578, 591, 1211
AlwNI	CAGNNNCTG	1 cut(s)	1603
AoxI	GGCC	4 cut(s)	303, 354, 924, 1634
ApeKI	GCWGC	2 cut(s)	338, 1097
ApoI	RAATTY	8 cut(s)	214, 1064, 1342, 1357, 1366, 1431, 1442, 1565
AseI	ATTAAT	1 cut(s)	1103
Asp700I	GAANNNNTTC	2 cut(s)	504, 1489
AspLEI	GCGC	2 cut(s)	192, 256
AspS9I	GGNCC	1 cut(s)	1635
AsuHPI	GGTGA	5 cut(s)	158, 159, 283, 671, 902
AxyI	CCTNAGG	2 cut(s)	543, 1461
BalI	TGGCCA	1 cut(s)	305
BamHI	GGATCC	1 cut(s)	583
Bbv12I	GWGCWC	1 cut(s)	1201
BbvI	GCAGC	2 cut(s)	325, 1109
BccI	CCATC	4 cut(s)	805, 887, 1086, 1231
BcgI	CGANNNNNNTGC	4 cut(s)	249, 283, 704, 738
BciT130I	CCWGG	1 cut(s)	597
BclI	TGATCA	1 cut(s)	385
BcoDI	GTCTC	1 cut(s)	72
BcuI	ACTAGT	2 cut(s)	523, 1201
BfaI	CTAG	2 cut(s)	524, 1202
BfmI	CTRYAG	1 cut(s)	240
BisI	GCNGC	2 cut(s)	339, 1098
BlsI	GCNGC	2 cut(s)	340, 1099
Bme1390I	CCNGG	1 cut(s)	597
BmgT120I	GGNCC	1 cut(s)	1635
BmiI	GGNNCC	4 cut(s)	585, 712, 1266, 1637
BmrFI	CCNGG	1 cut(s)	597
BmrI	ACTGGG	1 cut(s)	1066
BmsI	GCATC	6 cut(s)	367, 598, 712, 895, 1227, 1633
BmuI	ACTGGG	1 cut(s)	1066
BpmI	CTGGAG	1 cut(s)	54
BpuEI	CTTGAG	2 cut(s)	297, 1176
BsaJI	CCNNGG	2 cut(s)	1348, 1558
BsaXI	ACNNNNNCTCC	2 cut(s)	1188, 1218
Bse1I	ACTGG	5 cut(s)	71, 332, 892, 1072, 1664
Bse21I	CCTNAGG	2 cut(s)	543, 1461
Bse3DI	GCAATG	2 cut(s)	99, 405
BseBI	CCWGG	1 cut(s)	597
BseDI	CCNNGG	2 cut(s)	1348, 1558
BseGI	GGATG	3 cut(s)	409, 553, 1003
BseMI	GCAATG	2 cut(s)	99, 405
BseMII	CTCAG	4 cut(s)	126, 534, 786, 1452
BseNI	ACTGG	5 cut(s)	71, 332, 892, 1072, 1664
BseXI	GCAGC	2 cut(s)	325, 1109
BseYI	CCCAGC	1 cut(s)	192
BsgI	GTGCAG	1 cut(s)	1178
Bsh1236I	CGCG	1 cut(s)	61
BshFI	GGCC	4 cut(s)	305, 356, 926, 1636
BsiHKAI	GWGCWC	1 cut(s)	1201
BslFI	GGGAC	2 cut(s)	828, 882
BsmAI	GTCTC	1 cut(s)	72
BsmFI	GGGAC	2 cut(s)	828, 882
BsnI	GGCC	4 cut(s)	305, 356, 926, 1636
Bsp1286I	GDGCHC	1 cut(s)	1201
Bsp143I	GATC	7 cut(s)	164, 385, 583, 818, 1040, 1216, 1255
Bsp68I	TCGCGA	1 cut(s)	61
BspACI	CCGC	2 cut(s)	463, 1240
BspANI	GGCC	4 cut(s)	305, 356, 926, 1636
BspCNI	CTCAG	4 cut(s)	127, 535, 787, 1453
BspFNI	CGCG	1 cut(s)	61
BspLI	GGNNCC	4 cut(s)	585, 712, 1266, 1637
BspPI	GGATC	4 cut(s)	172, 578, 591, 1211
BsrDI	GCAATG	2 cut(s)	99, 405
BsrI	ACTGG	5 cut(s)	71, 332, 892, 1072, 1664
BssECI	CCNNGG	2 cut(s)	1348, 1558
BssMI	GATC	7 cut(s)	164, 385, 583, 818, 1040, 1216, 1255
BssT1I	CCWWGG	1 cut(s)	1558
Bst2UI	CCWGG	1 cut(s)	597
Bst4CI	ACNGT	5 cut(s)	7, 244, 1087, 1516, 1579
Bst6I	CTCTTC	1 cut(s)	745
BstC8I	GCNNGC	2 cut(s)	258, 852
BstDEI	CTNAG	4 cut(s)	135, 543, 795, 1461
BstDSI	CCRYGG	1 cut(s)	1348
BstF5I	GGATG	3 cut(s)	409, 553, 1003
BstFNI	CGCG	1 cut(s)	61
BstHHI	GCGC	2 cut(s)	192, 256
BstKTI	GATC	7 cut(s)	167, 388, 586, 821, 1043, 1219, 1258
BstMAI	GTCTC	1 cut(s)	72
BstMBI	GATC	7 cut(s)	164, 385, 583, 818, 1040, 1216, 1255
BstMWI	GCNNNNNNNGC	1 cut(s)	965
BstNI	CCWGG	1 cut(s)	597
BstNSI	RCATGY	2 cut(s)	133, 875
BstSCI	CCNGG	1 cut(s)	595
BstSFI	CTRYAG	1 cut(s)	240
BstUI	CGCG	1 cut(s)	61
BstV1I	GCAGC	2 cut(s)	325, 1109
BstX2I	RGATCY	1 cut(s)	583
BstXI	CCANNNNNNTGG	2 cut(s)	78, 632
BstYI	RGATCY	1 cut(s)	583
Bsu36I	CCTNAGG	2 cut(s)	543, 1461
BsuRI	GGCC	4 cut(s)	305, 356, 926, 1636
BtgI	CCRYGG	1 cut(s)	1348
BtsCI	GGATG	3 cut(s)	409, 553, 1003
BtsI	GCAGTG	2 cut(s)	127, 1604
BtsIMutI	CAGTG	8 cut(s)	78, 127, 325, 813, 854, 885, 1521, 1604
BtuMI	TCGCGA	1 cut(s)	61
Cac8I	GCNNGC	2 cut(s)	258, 852
CaiI	CAGNNNCTG	1 cut(s)	1603
CfoI	GCGC	2 cut(s)	192, 256
Cfr13I	GGNCC	1 cut(s)	1635
Csp6I	GTAC	2 cut(s)	370, 1212
CviAII	CATG	7 cut(s)	130, 251, 360, 649, 872, 1117, 1183
CviQI	GTAC	2 cut(s)	370, 1212
DdeI	CTNAG	4 cut(s)	135, 543, 795, 1461
DpnI	GATC	7 cut(s)	166, 387, 585, 820, 1042, 1218, 1257
DpnII	GATC	7 cut(s)	164, 385, 583, 818, 1040, 1216, 1255
EaeI	YGGCCR	1 cut(s)	303
Eam1104I	CTCTTC	1 cut(s)	745
EarI	CTCTTC	1 cut(s)	745
Eco130I	CCWWGG	1 cut(s)	1558
Eco32I	GATATC	1 cut(s)	804
Eco57I	CTGAAG	4 cut(s)	30, 878, 981, 1520
Eco81I	CCTNAGG	2 cut(s)	543, 1461
EcoRI	GAATTC	1 cut(s)	1442
EcoRII	CCWGG	1 cut(s)	595
EcoRV	GATATC	1 cut(s)	804
EcoT14I	CCWWGG	1 cut(s)	1558
EcoT22I	ATGCAT	1 cut(s)	650
ErhI	CCWWGG	1 cut(s)	1558
FaeI	CATG	7 cut(s)	133, 254, 363, 652, 875, 1120, 1186
FalI	AAGNNNNNCTT	1 cut(s)	1679
FaqI	GGGAC	2 cut(s)	828, 882
FatI	CATG	7 cut(s)	129, 250, 359, 648, 871, 1116, 1182
FbaI	TGATCA	1 cut(s)	385
FblI	GTMKAC	1 cut(s)	126
Fnu4HI	GCNGC	2 cut(s)	339, 1098
FokI	GGATG	3 cut(s)	416, 560, 1010
Fsp4HI	GCNGC	2 cut(s)	339, 1098
FspBI	CTAG	2 cut(s)	524, 1202
GlaI	GCGC	2 cut(s)	191, 255
GluI	GCNGC	2 cut(s)	339, 1098
GsaI	CCCAGC	1 cut(s)	196
GsuI	CTGGAG	1 cut(s)	54
HaeIII	GGCC	4 cut(s)	305, 356, 926, 1636
HhaI	GCGC	2 cut(s)	192, 256
Hin1II	CATG	7 cut(s)	133, 254, 363, 652, 875, 1120, 1186
Hin6I	GCGC	2 cut(s)	190, 254
HinP1I	GCGC	2 cut(s)	190, 254
HindIII	AAGCTT	1 cut(s)	836
HinfI	GANTC	3 cut(s)	236, 600, 1162
HphI	GGTGA	5 cut(s)	158, 159, 283, 671, 902
Hpy166II	GTNNAC	1 cut(s)	127
Hpy188III	TCNNGA	3 cut(s)	60, 453, 1651
Hpy8I	GTNNAC	1 cut(s)	127
HpyAV	CCTTC	6 cut(s)	54, 740, 1015, 1254, 1347, 1690
HpyCH4III	ACNGT	5 cut(s)	7, 244, 1087, 1516, 1579
HpyF10VI	GCNNNNNNNGC	1 cut(s)	965
HpyF3I	CTNAG	4 cut(s)	135, 543, 795, 1461
Hsp92II	CATG	7 cut(s)	133, 254, 363, 652, 875, 1120, 1186
HspAI	GCGC	2 cut(s)	190, 254
Ksp22I	TGATCA	1 cut(s)	385
Kzo9I	GATC	7 cut(s)	164, 385, 583, 818, 1040, 1216, 1255
LmnI	GCTCC	3 cut(s)	913, 1147, 1196
Lsp1109I	GCAGC	2 cut(s)	325, 1109
LweI	GCATC	6 cut(s)	367, 598, 712, 895, 1227, 1633
MaeI	CTAG	2 cut(s)	524, 1202
MaeIII	GTNAC	4 cut(s)	652, 659, 883, 1321
MalI	GATC	7 cut(s)	166, 387, 585, 820, 1042, 1218, 1257
MboI	GATC	7 cut(s)	164, 385, 583, 818, 1040, 1216, 1255
MfeI	CAATTG	2 cut(s)	684, 1014
MflI	RGATCY	1 cut(s)	583
MhlI	GDGCHC	1 cut(s)	1201
MlsI	TGGCCA	1 cut(s)	305
MluNI	TGGCCA	1 cut(s)	305
MlyI	GAGTC	1 cut(s)	609
MmeI	TCCRAC	2 cut(s)	533, 1069
Mox20I	TGGCCA	1 cut(s)	305
Mph1103I	ATGCAT	1 cut(s)	650
MroXI	GAANNNNTTC	2 cut(s)	504, 1489
MscI	TGGCCA	1 cut(s)	305
MseI	TTAA	9 cut(s)	86, 201, 264, 297, 416, 639, 1103, 1148, 1587
MslI	CAYNNNNRTG	1 cut(s)	1181
Msp20I	TGGCCA	1 cut(s)	305
MspR9I	CCNGG	1 cut(s)	597
MunI	CAATTG	2 cut(s)	684, 1014
MvaI	CCWGG	1 cut(s)	597
MvnI	CGCG	1 cut(s)	61
MwoI	GCNNNNNNNGC	1 cut(s)	965
NdeII	GATC	7 cut(s)	164, 385, 583, 818, 1040, 1216, 1255
NlaIII	CATG	7 cut(s)	133, 254, 363, 652, 875, 1120, 1186
NlaIV	GGNNCC	4 cut(s)	585, 712, 1266, 1637
NmuCI	GTSAC	3 cut(s)	659, 883, 1321
NruI	TCGCGA	1 cut(s)	61
NsiI	ATGCAT	1 cut(s)	650
NspI	RCATGY	2 cut(s)	133, 875
PciI	ACATGT	2 cut(s)	129, 871
PdmI	GAANNNNTTC	2 cut(s)	504, 1489
PfeI	GAWTC	2 cut(s)	236, 1162
PkrI	GCNGC	2 cut(s)	340, 1099
PleI	GAGTC	1 cut(s)	608
PpsI	GAGTC	1 cut(s)	608
PscI	ACATGT	2 cut(s)	129, 871
PshBI	ATTAAT	1 cut(s)	1103
Psp6I	CCWGG	1 cut(s)	595
PspFI	CCCAGC	1 cut(s)	192
PspGI	CCWGG	1 cut(s)	595
PspN4I	GGNNCC	4 cut(s)	585, 712, 1266, 1637
PspPI	GGNCC	1 cut(s)	1635
PstNI	CAGNNNCTG	1 cut(s)	1603
PsuI	RGATCY	1 cut(s)	583
RruI	TCGCGA	1 cut(s)	61
RsaI	GTAC	2 cut(s)	371, 1213
RsaNI	GTAC	2 cut(s)	370, 1212
RseI	CAYNNNNRTG	1 cut(s)	1181
SaqAI	TTAA	9 cut(s)	86, 201, 264, 297, 416, 639, 1103, 1148, 1587
SatI	GCNGC	2 cut(s)	339, 1098
Sau3AI	GATC	7 cut(s)	164, 385, 583, 818, 1040, 1216, 1255
Sau96I	GGNCC	1 cut(s)	1635
SchI	GAGTC	1 cut(s)	609
ScrFI	CCNGG	1 cut(s)	597
SduI	GDGCHC	1 cut(s)	1201
SfaNI	GCATC	6 cut(s)	367, 598, 712, 895, 1227, 1633
SfcI	CTRYAG	1 cut(s)	240
SmiMI	CAYNNNNRTG	1 cut(s)	1181
SmlI	CTYRAG	2 cut(s)	312, 1191
SmoI	CTYRAG	2 cut(s)	312, 1191
SpeI	ACTAGT	2 cut(s)	523, 1201
SsiI	CCGC	2 cut(s)	463, 1240
SspI	AATATT	1 cut(s)	1057
SspMI	CTAG	2 cut(s)	524, 1202
StyD4I	CCNGG	1 cut(s)	595
StyI	CCWWGG	1 cut(s)	1558
TaaI	ACNGT	5 cut(s)	7, 244, 1087, 1516, 1579
TaqI	TCGA	5 cut(s)	616, 1039, 1258, 1453, 1551
TatI	WGTACW	1 cut(s)	369
TfiI	GAWTC	2 cut(s)	236, 1162
Tru1I	TTAA	9 cut(s)	86, 201, 264, 297, 416, 639, 1103, 1148, 1587
Tru9I	TTAA	9 cut(s)	86, 201, 264, 297, 416, 639, 1103, 1148, 1587
TscAI	CASTG	8 cut(s)	78, 127, 332, 813, 861, 892, 1521, 1604
TseFI	GTSAC	3 cut(s)	659, 883, 1321
TseI	GCWGC	2 cut(s)	338, 1097
Tsp45I	GTSAC	3 cut(s)	659, 883, 1321
TspDTI	ATGAA	9 cut(s)	227, 239, 308, 420, 444, 531, 1409, 1455, 1672
TspGWI	ACGGA	2 cut(s)	1365, 1600
TspRI	CASTG	8 cut(s)	78, 127, 332, 813, 861, 892, 1521, 1604
VspI	ATTAAT	1 cut(s)	1103
XapI	RAATTY	8 cut(s)	214, 1064, 1342, 1357, 1366, 1431, 1442, 1565
XceI	RCATGY	2 cut(s)	133, 875
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1085
XmiI	GTMKAC	1 cut(s)	126
XmnI	GAANNNNTTC	2 cut(s)	504, 1489
XspI	CTAG	2 cut(s)	524, 1202
Zsp2I	ATGCAT	1 cut(s)	650

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.