Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003103

Physical Location & Seq

Genomic Coordinates Reverse (-)

26793550 .. 26829498

Gene Structure 35949 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1245600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6189 bp

ATGAATCAATCGAGAGGCTCACCTTCGGATTTGAAGGCCAGCGGCAGCCGCTCCGGTGACTTGGACGGCGAGTCCTCGTCCAGTCCGTTTTATATTGCCAGAACTAAGGATGCTCCGGTCCATCGCCTCAAGCGCTGGAGGCATGCGGCACTTGTGCTGAATGCTTCTCGTCGATTCCGGTACACCTTAGACTTGAAAAAGGAAGAAGAAAAGCAGCATACACTAAGGAAGATTAAAGCACATGCACAAGCCATACGAGTAATTCACTTCAAAATAAACGTGTCATATGTCACTCATGCTGCATATCTTTTCAAGGAAGCTGCAGATCAACAAGTGAATGGGATTGTAGTACCGAAACCAAGTCCTACTGGCGATTTTCCCATTGGACAAGAGGAACTAGTTTCAGTGACGAGGGATCATAAATTTGCTGCTCTTCAACAATATGGGGGGGTCACAGGCTTAGGGGACTTGTTGAAAACAAATCTCGACAAGGGAATTCATGGAGATGATATTGATTTACTAAAACGGAAAAATGCATTTGGGTCGAACACATATCCTCGGAAAAAGCAAGAAGTTTCTGGATTTCTTTGGGTAGCTTGTCAAGATCTTACTCTGATCATATTGATGGTAGCTGCAATGGCTTCTTTAGCGCTGGGTATAAAAACAGAGGGTACAGAGGAGGGATGGTCTGATGGGGGCTGCATTGCTTTTGCAGTTCTCCTTGTCATTGTTGTCACAGCCGTTAGTGATTACAGACAATCTCTTCAGTTCCAGAATCTAACTGAGGAAAAGAGAAATATACATTTGGAGGTCATTAGAGGTGGTAGACGTGTTAAGGTCTCAATTTATGATCTTGTTGTTGGTGATGTTGTACCCCTTAACATCGGCGATCAGGTCCCTGCTGACGGCATTTTAATAATTGGACACTCACTTTTGATAGATGAGTCTAGTATGACTGGAGAGAGCAAGATTGTACGCAAGAACTCCAAAAATCCATTTCTAATGTCGGGCTGCAAAGTAGCAGTTGGAAATGGTAGCATGCTGGTAACCAGTGTTGGGACTAATACTGAATGGGGAATGCTCATGGCTAGTATTTCAGAAGATACTGGTGAAGAAACTCCTTTGCAGGTGCGCTTAAACGGGGTTGCAACTTCCATTGGTATTGTTGGACTCACAGTGGCTTTACCTGTCTTGATAGTCCTATTGTTTAGTTACTTTAGTGGCCATACAAAAAATGCAAATGGAATCCCTCAGTTTCAAGTGGGAAAGACAAAATTTGGAGATGGCATAGATGGGGCCATTAAAATTGTTATTATTGCGGTGACCATTGTCGTGGCTGGTGTTCCTGAAGGGCTCCCATTATCTGTTACCTTGACGCTTGCCTACTCGATGAGAAAATTGATGGCAGATAAGGCCTTGGTGCGGAGGCTGTCTGCATGTGAAACCATGGGCTTTGCAACCGCTATATGCTGCGACAAGACTGGAACCTTAACATTGAACCAGGGGAAGACACTGGTGGTTTGTGTCTCTCTTGCTCTGTCAATGACTGTTGTTGAGGCTTTTACTGGTGGGAGGAAAATCGATCTCTCTGGCAGTAAGTCAGACGTGTCTCCAATACTATCATCTTTGCTTATTGAAGGCATTTCATTGAATACAAATGGCAGTGTATATACGTCTGAGACTGGCGGTGATATAGAGGTTTCTGGATCACCAACTGAAAAGGCAATTCTGCAATTGGGAATCAAGCTCGGAATGAATTTTGATGCCATCAGGTCCAAGTCTTCAATTCTTCACGTGTTCCCATTCAACTCAGAGAAAAAACGAGGTGGTGCTGCAGTTAAATTGCCCAATACCGAAGTTCATATTCACTGGAAAGGGGCTGCTGAAATAGTCTTGGCCTGTTGTACAAAGTACCTGGATCCTAATGACCAGTTAGCAGCTATGGATGAAGACAAGTCAACCATGTTCAGGAAGTCAATTGAGGATATGGCTGCCCGCAGTTTGCGTTGTGTTGCCATTGCTTATAGACCACATGAATTGGAAAATGTTCCAGCTGATGAACAACTATCTGAGTGGGCATTGCCTGATGATGATCTTGTCTTGCTTGCAATTGTTGGTATAAAGGATCCTTGTCGGCCTGGGGTGGGAGATGCAGTGCGACTATGCCAAAAAGCTGGTGTTAAGGTAATCATGGTCACAGGTGACAATCTTCAAACTGCGAAAGCGATTGCTATGGAATGTGGCATACTTGCTCCAGATTCAGATGCTACCCAGCCAACTCTCATTGAAGGAACAGCTTTCCGTGAACTTTCAGATAAGCAGAGAGAAGATTGTGCTGAAAAGATTTCGGTGATGGGGCGGTCTTCCCACAATGACAAGCTTCTGCTTGTGCAAGCATTGAGGAGAAGGGGTCATGTTGTTGCTGTAACTGGAGATGGCACTAATGATGCTCCTGCACTGCGCGAGGCTGACATTGGTCTTGCAATGGGTATTCAAGGGACTGAAGTTGCCAAAGAGAATTCAGATATCATTATCTTGGACGATAATTTTGCTTCAGTTGTGAAGGTTGTTAGATGGGGTCGATCTATATACGAAGGTATTCAGAAATTCACCCAATTCCATCTTACTACACTCAATGTTGCAGCAGTAACTATAAATGCCGTGGCAGCCATTTCATCTGGTGATGTACCGCTAAATGCTGTTCAGGTTCTGTGGGTGAATCTTATCATGGATACTGTTGGAGTACTAGCATTGGCTACGGAGCCACCTACAGATCACTTGATGGACAGACCCCCTGTTGGTCAAAGAGAGCCGCTCATAACAAATATAATGTGGAGGAACTTGTTGGTTCAGGCTTTCTACCAAGTCACTGTCCTACTTGTCCTCAACTTCCGAGGAATAAGTATACTGAAGCTACATGGTCCCAATGCTAACACAGTGAAAAATACTCTGATATTCAATACATTTGTCCTATGTCAAATTTTCAATGAATTCATTGCTCAAAATCCTGATGAGTTCAATATATTCAAAGGGATCACCAAGAACTACCTTTTTATAGGAATAGTGACAGTAACTCTTGTACTTCAGATTATCATCGTCGAGTTCCTTGGGAAATTCACTAAAACAAAACCACTTAATTGGAATCTTTGGCTGATTTCAGCTGTAATAGCTTTTATCAGTTGGCCTCTTGCTGTTCTTGGGAAACTGATTCCGGTTCCAGAAACCCCTCTCTTCAAGAATTTTACAAGAATGATCCCTGGGCGAAGAAACAGTCCAGAGGCTGCATATCTTTTCAAGGAAGCTGCAGATCAACAAGTGAATGGGATTGTAGTACCGAAACCAAGTCCTACTGGCGATTTTCCCATTGGACAAGAGGAACTAGTTTCAGTGACGAGGGATCATAAATTTGCTGCTCTTCAACAATATGGGGGGGTCACAGGCTTAGGGGACTTGTTGAAAACAAATCTCGACAAGGGAATTCATGGAGATGATATTGATTTACTAAAACGGAAAAATGCATTTGGGTCGAACACATATCCTCGGAAAAAAGCAAGAAGTTTCTGGAGATTTCTTTGGGTAGCTTGTCAAGATCTTACTCTGATCATATTGATGGTAGCTGCAATGGCTTCTTTAGCGCTGGGTATAAAAACAGAGGGTATAGAGGAGGGATGGTATGATGGAGGCAGCATTGCTTTTGCACTTCTCCTTGTCATTGTTGTCACAGCGGTTAGTGATTACAGACAATCTCTTAAGTTCCAGAATCTAAATGAGGAAAAGAGAAATATACATTTGGAGGTCATTAGAGGTGGTAGACGTGTTGAGGTCTCAATTTATGATCTTGTTGTTGGTGATGTTGCACCCCTTAACATCGGCGATCAGGTCCCTGCTGATGGCATTTTAATAACTGGACACTCACTTGCCATAGATGAGTCTAGTATGACTGGAGAGAGCAAGATTGTACGCAAGGACTCCAAAAATCCATTTCTAATGTCAGGCTGCAAAGTAGCAGATGGAAATGGTAGCATGCTGGTAACCAGTGTTGGGACTAATACTGAATGGGGAATGCTCATGGCTAGTATTTCAGAAGATACTGGTGAGGAAACTCCTTTGCAGGTGCGCTTAAACGGGGTTGCAACTTTCATTGGTATTGTTGGACTCACAGTGGCTTTACTTGTCTTGATAGTCCTGTTGGTTAGATACTTTACTGGCCATACAAAAAATGCAAATGGAAGCCCTCAGTTTAAAGCGGGAAAGACAAAATTTGGAGATGCCATAGATGGAGCCATTAAAATTGTTACTATTGCGGTCACCATTGTGGTGGTTGCTGTTCCTGAAGGGCTCCCATTAGCTGTTACCTTGACGCTTGCCTACTCGATGAGAAAAATGATGGCAGATAAGGCCTTGGTGCGGAGGCTGTCTGCATGTGAAACCATGGGCTCTGCAACTACCATATGCAGCGACAAGACTGGAACCTTAACATTGAACCAGATGACTGTTGTTGAGGCTTTTACTGGTGGGAGGAAAATCGATCTCTCTGGCAGCATTGCGTTGAATACAAATGGCAGTGTCTATACGCCTGAGACTGGCGGTGATATAGAGGTTTCTGGATCACCAACTGAAAAGGCAATTCTGCAATTGGGAATCAAGCTCGGAATGAATTTTGATGCCATCAGGTCCAAGTCTTCAATTCTTCACGTGTTCCCATTCAACTCAGAGAAAAAACGAGGCGGTGCTGCAGTTAAATTGCCCAATACCGAAGTTCATATTCAATGGAAAGGGGCTGCTGAAATAGTCTTGGCCTGTTGTACAAAGTACCTGGATCCTAATGACCAGTTAGTAGCTATGGATGAAGACAAGTCAACCATGTTCAGGAAGTCAATTGAGGATATGGCTGCTCGCAGTTTGCGTTGTGTTGCCATTGCTTATAGACCACATGAATTGGAAAATGTTCCAGCTGATGAACAACTATCTGAGTGGGCATTGCCTGATGATGACCTTGTCTTGCTTGCTATTGTTGGTATAAAGGATCCTTGTCGGCCTGGGGTGGGAGATGCAGTGCGACTATGCCAAAAAGCTGGTGTTAAGGTACGCATGGTCACAGGTGACAATCTTCAAACTGCGAAAGCGATTGCTATGGAATGTGGCATACTTGCTCCAGATTCAGATGCTAACCAGCCAACTCTCATTGAAGGAACAGCTTTCCGTGAACTTTCAGATAAGCAGAGAGAAGATTGTGCTGAAAAGATTTCGGTGATGGGGCGGTCTTCCCCCAATGACAAGCTTCTGCTTGTGCAAGCATTGAGGAGAAGGGGTCATGTTGTTGCTGTAACTGGAGATGGCACTAATGATGCTCCTGCACTGCACGAGGCTGACATCGGTCTTGCAATGGGTATTCAAGGGACTGAAGTTGCCAAAGAGAGTTCAGATATCATTATCTTGGACGATAATTTTGCTTCAGTTGTGAAGGTTGTTAGATGGGGTCGATCTGTATATGCAAATATTCAGAAATTCATCCAATTCCAGCTTACAGTAAATGTAGCAGCACTAATTATAAATGTCGTCGCAGCCATTTCATCTGGTGATGTACCGCTAAATGCTGTTCAGCTTCTGTGGGTGAATCTTATCATGGATACTCTTGGAGCACTAGCATTAGCTACGGAGCCACCTACAGATCACTTGATGGACAGACCCCCTGTTGGTCGAAGAGAGCCGCTCATAACAAATATAATGTGGAGGAACTTGTTGGTTCAGGCTTTCTACCAAGTCACTGTCCTACTTGTCTTCAACTTCCGAGGAATAAGTATACTGAAGCTACATGGTCCCAATGCTAACACAGTGAAGAATACTCTGATATTCAATACATTTGTCCTATGTCAGATTTTCAATGAATTCAATGCTCGAAAGCCTGATGAGTTCAATATATTCAAAGGGATCACCAAGAACTACCTTTTTATAGGAATAGTGACAGTAACTCTTGTACTTCAGATTATCATCGTCGAGTTCCTTGGGAAGTTCACTAAAACAAAACCACTTACTTGGAATCTTTGGCTAATTTCAGCTGTAATAGCTTTTATCAGTTGGCCTCTTGCTGTTCTCGGGAAGCTGATTCCGGTTCCAGAAACTCCTCTCTTCAAGTATTTTACAAGAATGATCCCTGGGCGAAGAAACAGTCCAGAGGGTAGTCGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

2063

Amino Acids

224.01

Weight (kDa)

7.67

Isoelectric Point (pI)

33.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	28 - 70	4.5e-14	Ca2+-ATPase N terminal autoinhibitory domain
E1-E2_ATPase	PF00122	269 - 364	4.8e-19	P-type ATPase actuator domain
Cation_ATPase	PF13246	548 - 713	1.9e-31	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	700 - 824	5.6e-12	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	896 - 1070	3.1e-40	Cation transporting ATPase, C-terminus
Cation_ATPase_N	PF00690	1143 - 1209	2.5e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	1264 - 1359	2.7e-19	P-type ATPase actuator domain
Hydrolase	PF00702	1483 - 1791	3.5e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	1516 - 1680	4.6e-31	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	1862 - 2036	2.3e-44	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29900 AT4G29900 AT5G57110 AT5G57110 AT5G57110
fragaria_vesca	FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940
malus_domestica	MD13G1241000.v1.1 MD16G1245600.v1.1 MD16G1246100.v1.1
prunus_persica	Prupe.1G072600_v2.0.a1 Prupe.8G012900_v2.0.a1
pyrus_communis	pycom13g21220
rosa_chinensis	RchiOBHm_Chr4g0398891 RchiOBHm_Chr4g0398951
rosa_laevigata	RLG00000009307 RLG00000009309 RLG00000009365
rosa_multiflora	Rmu_sc0004094.1_g000033 Rmu_ssc0000064.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343340 Rroxscaffold_5G00343930 Rroxscaffold_5G00344000
rosa_rugosa	Rorug04G0013200
rosa_samantha	Rh4AG091100 Rh4CG093100 Rh4CG099100 Rh4CG099400
rosa_wichuraiana	Rw4G007100 Rw4G007130 Rw4G007500 Rw4G007540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	5552
AarI	CACCTGC	2 cut(s)	1117, 4102
Acc36I	ACCTGC	2 cut(s)	1117, 4102
AccBSI	CCGCTC	3 cut(s)	51, 2815, 5713
AccI	GTMKAC	4 cut(s)	826, 2905, 3811, 5803
AccII	CGCG	1 cut(s)	2463
AcoI	YGGCCR	2 cut(s)	1222, 4207
AcvI	CACGTG	2 cut(s)	1795, 4696
AfeI	AGCGCT	3 cut(s)	134, 651, 3636
AflII	CTTAAG	1 cut(s)	3749
AflIII	ACRYGT	6 cut(s)	279, 829, 1605, 1794, 3814, 4695
AhdI	GACNNNNNGTC	1 cut(s)	70
AhlI	ACTAGT	2 cut(s)	397, 3379
AjiI	CACGTC	3 cut(s)	830, 1606, 3815
AjnI	CCWGG	7 cut(s)	1500, 1914, 2137, 3256, 4815, 5038, 6154
AleI	CACNNNNGTG	1 cut(s)	4313
Alw21I	GWGCWC	1 cut(s)	5644
Alw26I	GTCTC	6 cut(s)	844, 1531, 1614, 1673, 3829, 4574
AlwNI	CAGNNNCTG	3 cut(s)	2710, 3281, 5608
Ama87I	CYCGRG	1 cut(s)	6095
Aor51HI	AGCGCT	3 cut(s)	134, 651, 3636
ArsI	GACNNNNNNTTYG	8 cut(s)	2531, 2563, 3256, 3288, 5432, 5464, 6154, 6186
Asp700I	GAANNNNTTC	3 cut(s)	2046, 2598, 4947
AspLEI	GCGC	6 cut(s)	135, 652, 1134, 2463, 3637, 4119
AspS9I	GGNCC	8 cut(s)	118, 895, 1296, 1773, 2921, 3880, 4674, 5819
AvaI	CYCGRG	1 cut(s)	6095
AvaII	GGWCC	7 cut(s)	118, 895, 1773, 2921, 3880, 4674, 5819
BalI	TGGCCA	2 cut(s)	1224, 4209
BamHI	GGATCC	4 cut(s)	1918, 2125, 4819, 5026
BanII	GRGCYC	3 cut(s)	1356, 4341, 4439
BauI	CACGAG	1 cut(s)	5363
BbrPI	CACGTG	2 cut(s)	1795, 4696
BbsI	GAAGAC	8 cut(s)	1514, 1773, 1956, 2355, 4674, 4857, 5256, 5773
Bbv12I	GWGCWC	1 cut(s)	5644
BceAI	ACGGC	4 cut(s)	82, 725, 922, 2645
BcgI	CGANNNNNNTGC	8 cut(s)	525, 559, 2531, 2565, 3507, 3541, 5432, 5466
BciT130I	CCWGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
BciVI	GTATCC	2 cut(s)	2725, 5623
BclI	TGATCA	2 cut(s)	615, 3600
BcoDI	GTCTC	6 cut(s)	844, 1531, 1614, 1673, 3829, 4574
BcuI	ACTAGT	2 cut(s)	397, 3379
BfaI	CTAG	8 cut(s)	398, 948, 1089, 2747, 3380, 3933, 4074, 5645
BfmI	CTRYAG	6 cut(s)	321, 1833, 2769, 3303, 4734, 5667
BfoI	RGCGCY	3 cut(s)	136, 653, 3638
BfrI	CTTAAG	1 cut(s)	3749
BfuAI	ACCTGC	2 cut(s)	1117, 4102
BfuI	GTATCC	2 cut(s)	2725, 5623
BglII	AGATCT	2 cut(s)	604, 3589
BmcAI	AGTACT	1 cut(s)	2745
Bme1390I	CCNGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
Bme18I	GGWCC	7 cut(s)	118, 895, 1773, 2921, 3880, 4674, 5819
BmeRI	GACNNNNNGTC	1 cut(s)	70
BmeT110I	CYCGRG	1 cut(s)	6095
BmgBI	CACGTC	3 cut(s)	830, 1606, 3815
BmgT120I	GGNCC	8 cut(s)	118, 895, 1296, 1773, 2921, 3880, 4674, 5819
BmrFI	CCNGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
BoxI	GACNNNNGTC	3 cut(s)	1328, 2475, 5376
BpiI	GAAGAC	8 cut(s)	1514, 1773, 1956, 2355, 4674, 4857, 5256, 5773
BplI	GAGNNNNNCTC	4 cut(s)	2799, 2831, 5697, 5729
BpmI	CTGGAG	8 cut(s)	157, 978, 2238, 2451, 3583, 3963, 5139, 5352
Bpu10I	CCTNAGC	2 cut(s)	460, 3442
BpuEI	CTTGAG	1 cut(s)	113
Bsa29I	ATCGAT	2 cut(s)	1581, 4527
BsaAI	YACGTR	2 cut(s)	1795, 4696
BsaBI	GATNNNNATC	3 cut(s)	2091, 3092, 5990
BsaI	GGTCTC	2 cut(s)	844, 3829
BsaWI	WCCGGW	5 cut(s)	53, 115, 177, 3211, 6109
Bse8I	GATNNNNATC	3 cut(s)	2091, 3092, 5990
BseBI	CCWGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
BseCI	ATCGAT	2 cut(s)	1581, 4527
BseGI	GGATG	6 cut(s)	115, 689, 1951, 3674, 4852, 5511
BseJI	GATNNNNATC	3 cut(s)	2091, 3092, 5990
BseMII	CTCAG	9 cut(s)	774, 1265, 1668, 1824, 2061, 4250, 4569, 4725, 4962
BseRI	GAGGAG	5 cut(s)	692, 2416, 3677, 5317, 6114
BseYI	CCCAGC	3 cut(s)	652, 2271, 3637
BsgI	GTGCAG	3 cut(s)	2439, 5340, 5345
Bsh1236I	CGCG	1 cut(s)	2463
BshVI	ATCGAT	2 cut(s)	1581, 4527
BsiHKAI	GWGCWC	1 cut(s)	5644
BsiHKCI	CYCGRG	1 cut(s)	6095
BsiSI	CCGG	5 cut(s)	54, 116, 178, 3212, 6110
BsmAI	GTCTC	6 cut(s)	844, 1531, 1614, 1673, 3829, 4574
BsmI	GAATGC	3 cut(s)	166, 1083, 4068
Bso31I	GGTCTC	2 cut(s)	844, 3829
BsoBI	CYCGRG	1 cut(s)	6095
Bsp1286I	GDGCHC	4 cut(s)	1356, 4341, 4439, 5644
Bsp1407I	TGTACA	2 cut(s)	1904, 4805
Bsp19I	CCATGG	2 cut(s)	1446, 4431
BspCNI	CTCAG	9 cut(s)	775, 1264, 1669, 1823, 2062, 4249, 4570, 4724, 4963
BspDI	ATCGAT	2 cut(s)	1581, 4527
BspFNI	CGCG	1 cut(s)	2463
BspMAI	CTGCAG	4 cut(s)	325, 1837, 3307, 4738
BspMI	ACCTGC	2 cut(s)	1117, 4102
BspQI	GCTCTTC	2 cut(s)	438, 3420
BspTI	CTTAAG	1 cut(s)	3749
BspTNI	GGTCTC	2 cut(s)	844, 3829
BsrBI	CCGCTC	3 cut(s)	51, 2815, 5713
BsrGI	TGTACA	2 cut(s)	1904, 4805
BssNAI	GTATAC	2 cut(s)	2906, 5804
BssSI	CACGAG	1 cut(s)	5363
BssT1I	CCWWGG	6 cut(s)	1416, 1446, 3106, 4401, 4431, 6004
Bst1107I	GTATAC	2 cut(s)	2906, 5804
Bst2BI	CACGAG	1 cut(s)	5363
Bst2UI	CCWGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
Bst6I	CTCTTC	6 cut(s)	438, 768, 3236, 3420, 5698, 6134
BstAFI	CTTAAG	1 cut(s)	3749
BstAUI	TGTACA	2 cut(s)	1904, 4805
BstBAI	YACGTR	2 cut(s)	1795, 4696
BstDSI	CCRYGG	3 cut(s)	1446, 2661, 4431
BstEII	GGTNACC	4 cut(s)	1045, 1321, 4030, 4306
BstF5I	GGATG	6 cut(s)	115, 689, 1951, 3674, 4852, 5511
BstFNI	CGCG	1 cut(s)	2463
BstH2I	RGCGCY	3 cut(s)	136, 653, 3638
BstHHI	GCGC	6 cut(s)	135, 652, 1134, 2463, 3637, 4119
BstMAI	GTCTC	6 cut(s)	844, 1531, 1614, 1673, 3829, 4574
BstNI	CCWGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
BstNSI	RCATGY	6 cut(s)	146, 245, 1042, 1440, 4027, 4425
BstPAI	GACNNNNGTC	3 cut(s)	1328, 2475, 5376
BstPI	GGTNACC	4 cut(s)	1045, 1321, 4030, 4306
BstSCI	CCNGG	7 cut(s)	1500, 1914, 2137, 3256, 4815, 5038, 6154
BstSFI	CTRYAG	6 cut(s)	321, 1833, 2769, 3303, 4734, 5667
BstUI	CGCG	1 cut(s)	2463
BstV2I	GAAGAC	8 cut(s)	1514, 1773, 1956, 2355, 4674, 4857, 5256, 5773
BstX2I	RGATCY	6 cut(s)	604, 1918, 2125, 3589, 4819, 5026
BstXI	CCANNNNNNTGG	6 cut(s)	1333, 2174, 3138, 4318, 5075, 6036
BstYI	RGATCY	6 cut(s)	604, 1918, 2125, 3589, 4819, 5026
BstZ17I	GTATAC	2 cut(s)	2906, 5804
Bsu15I	ATCGAT	2 cut(s)	1581, 4527
BsuI	GTATCC	2 cut(s)	2725, 5623
BsuTUI	ATCGAT	2 cut(s)	1581, 4527
BtgI	CCRYGG	3 cut(s)	1446, 2661, 4431
BtgZI	GCGATG	1 cut(s)	107
BtrI	CACGTC	3 cut(s)	830, 1606, 3815
BtsCI	GGATG	6 cut(s)	115, 689, 1951, 3674, 4852, 5511
BtsI	GCAGTG	6 cut(s)	1669, 2160, 2456, 4570, 5061, 5357
BveI	ACCTGC	2 cut(s)	1117, 4102
CaiI	CAGNNNCTG	3 cut(s)	2710, 3281, 5608
CfoI	GCGC	6 cut(s)	135, 652, 1134, 2463, 3637, 4119
Cfr13I	GGNCC	8 cut(s)	118, 895, 1296, 1773, 2921, 3880, 4674, 5819
ClaI	ATCGAT	2 cut(s)	1581, 4527
CseI	GACGC	2 cut(s)	1384, 4369
CspCI	CAANNNNNGTGG	4 cut(s)	2019, 2054, 4920, 4955
DraI	TTTAAA	1 cut(s)	4243
DriI	GACNNNNNGTC	1 cut(s)	70
EaeI	YGGCCR	2 cut(s)	1222, 4207
Eam1104I	CTCTTC	6 cut(s)	438, 768, 3236, 3420, 5698, 6134
Eam1105I	GACNNNNNGTC	1 cut(s)	70
EarI	CTCTTC	6 cut(s)	438, 768, 3236, 3420, 5698, 6134
Eco130I	CCWWGG	6 cut(s)	1416, 1446, 3106, 4401, 4431, 6004
Eco147I	AGGCCT	2 cut(s)	1415, 4400
Eco24I	GRGCYC	3 cut(s)	1356, 4341, 4439
Eco31I	GGTCTC	2 cut(s)	844, 3829
Eco32I	GATATC	2 cut(s)	2527, 5428
Eco47I	GGWCC	7 cut(s)	118, 895, 1773, 2921, 3880, 4674, 5819
Eco47III	AGCGCT	3 cut(s)	134, 651, 3636
Eco72I	CACGTG	2 cut(s)	1795, 4696
Eco88I	CYCGRG	1 cut(s)	6095
Eco91I	GGTNACC	4 cut(s)	1045, 1321, 4030, 4306
EcoO109I	RGGNCCY	2 cut(s)	895, 3880
EcoO65I	GGTNACC	4 cut(s)	1045, 1321, 4030, 4306
EcoRI	GAATTC	5 cut(s)	495, 2518, 2990, 3477, 5888
EcoRII	CCWGG	7 cut(s)	1500, 1914, 2137, 3256, 4815, 5038, 6154
EcoRV	GATATC	2 cut(s)	2527, 5428
EcoT14I	CCWWGG	6 cut(s)	1416, 1446, 3106, 4401, 4431, 6004
EcoT22I	ATGCAT	2 cut(s)	538, 3520
EcoT38I	GRGCYC	3 cut(s)	1356, 4341, 4439
ErhI	CCWWGG	6 cut(s)	1416, 1446, 3106, 4401, 4431, 6004
FauI	CCCGC	2 cut(s)	2003, 4240
FauNDI	CATATG	2 cut(s)	286, 4451
FbaI	TGATCA	2 cut(s)	615, 3600
FblI	GTMKAC	4 cut(s)	826, 2905, 3811, 5803
FokI	GGATG	6 cut(s)	122, 696, 1958, 3681, 4859, 5498
FriOI	GRGCYC	3 cut(s)	1356, 4341, 4439
FspBI	CTAG	8 cut(s)	398, 948, 1089, 2747, 3380, 3933, 4074, 5645
GlaI	GCGC	6 cut(s)	134, 651, 1133, 2462, 3636, 4118
GsaI	CCCAGC	3 cut(s)	656, 2275, 3641
GsuI	CTGGAG	8 cut(s)	157, 978, 2238, 2451, 3583, 3963, 5139, 5352
HaeII	RGCGCY	3 cut(s)	136, 653, 3638
HapII	CCGG	5 cut(s)	54, 116, 178, 3212, 6110
HgaI	GACGC	2 cut(s)	1384, 4369
HhaI	GCGC	6 cut(s)	135, 652, 1134, 2463, 3637, 4119
Hin6I	GCGC	6 cut(s)	133, 650, 1132, 2461, 3635, 4117
HinP1I	GCGC	6 cut(s)	133, 650, 1132, 2461, 3635, 4117
HincII	GTYRAC	2 cut(s)	1959, 4860
HindII	GTYRAC	2 cut(s)	1959, 4860
HindIII	AAGCTT	2 cut(s)	2378, 5279
HpaII	CCGG	5 cut(s)	54, 116, 178, 3212, 6110
Hpy99I	CGWCG	4 cut(s)	174, 3101, 5564, 5999
HpyCH4IV	ACGT	7 cut(s)	279, 829, 1605, 1673, 1794, 3814, 4695
HpySE526I	ACGT	7 cut(s)	279, 829, 1605, 1673, 1794, 3814, 4695
HspAI	GCGC	6 cut(s)	133, 650, 1132, 2461, 3635, 4117
Ksp22I	TGATCA	2 cut(s)	615, 3600
LguI	GCTCTTC	2 cut(s)	438, 3420
MaeI	CTAG	8 cut(s)	398, 948, 1089, 2747, 3380, 3933, 4074, 5645
MaeII	ACGT	7 cut(s)	279, 829, 1605, 1673, 1794, 3814, 4695
MbiI	CCGCTC	3 cut(s)	51, 2815, 5713
MfeI	CAATTG	5 cut(s)	1733, 1977, 2109, 4634, 4878
MflI	RGATCY	6 cut(s)	604, 1918, 2125, 3589, 4819, 5026
MhlI	GDGCHC	4 cut(s)	1356, 4341, 4439, 5644
MlsI	TGGCCA	2 cut(s)	1224, 4209
MluNI	TGGCCA	2 cut(s)	1224, 4209
MlyI	GAGTC	6 cut(s)	80, 953, 1164, 3938, 3962, 4149
MmeI	TCCRAC	4 cut(s)	1006, 1147, 2719, 4132
Mox20I	TGGCCA	2 cut(s)	1224, 4209
Mph1103I	ATGCAT	2 cut(s)	538, 3520
MroXI	GAANNNNTTC	3 cut(s)	2046, 2598, 4947
MscI	TGGCCA	2 cut(s)	1224, 4209
MslI	CAYNNNNRTG	7 cut(s)	504, 623, 1331, 3486, 3608, 4313, 4316
Msp20I	TGGCCA	2 cut(s)	1224, 4209
MspA1I	CMGCKG	6 cut(s)	42, 2054, 3161, 3725, 4955, 6059
MspCI	CTTAAG	1 cut(s)	3749
MspI	CCGG	5 cut(s)	54, 116, 178, 3212, 6110
MspR9I	CCNGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
MunI	CAATTG	5 cut(s)	1733, 1977, 2109, 4634, 4878
Mva1269I	GAATGC	3 cut(s)	166, 1083, 4068
MvaI	CCWGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
MvnI	CGCG	1 cut(s)	2463
NcoI	CCATGG	2 cut(s)	1446, 4431
NdeI	CATATG	2 cut(s)	286, 4451
NsiI	ATGCAT	2 cut(s)	538, 3520
NspI	RCATGY	6 cut(s)	146, 245, 1042, 1440, 4027, 4425
OliI	CACNNNNGTG	1 cut(s)	4313
PaeI	GCATGC	3 cut(s)	146, 1042, 4027
PaqCI	CACCTGC	2 cut(s)	1117, 4102
PasI	CCCWGGG	2 cut(s)	3257, 6155
PceI	AGGCCT	2 cut(s)	1415, 4400
PciSI	GCTCTTC	2 cut(s)	438, 3420
PcsI	WCGNNNNNNNCGW	2 cut(s)	83, 4903
PctI	GAATGC	3 cut(s)	166, 1083, 4068
PdmI	GAANNNNTTC	3 cut(s)	2046, 2598, 4947
PflFI	GACNNNGTC	1 cut(s)	4997
PleI	GAGTC	6 cut(s)	79, 952, 1164, 3937, 3962, 4149
PmaCI	CACGTG	2 cut(s)	1795, 4696
PmlI	CACGTG	2 cut(s)	1795, 4696
PpsI	GAGTC	6 cut(s)	79, 952, 1164, 3937, 3962, 4149
Ppu21I	YACGTR	2 cut(s)	1795, 4696
PpuMI	RGGWCCY	2 cut(s)	895, 3880
PshAI	GACNNNNGTC	3 cut(s)	1328, 2475, 5376
PsiI	TTATAA	1 cut(s)	5552
Psp5II	RGGWCCY	2 cut(s)	895, 3880
Psp6I	CCWGG	7 cut(s)	1500, 1914, 2137, 3256, 4815, 5038, 6154
PspCI	CACGTG	2 cut(s)	1795, 4696
PspEI	GGTNACC	4 cut(s)	1045, 1321, 4030, 4306
PspFI	CCCAGC	3 cut(s)	652, 2271, 3637
PspGI	CCWGG	7 cut(s)	1500, 1914, 2137, 3256, 4815, 5038, 6154
PspPI	GGNCC	8 cut(s)	118, 895, 1296, 1773, 2921, 3880, 4674, 5819
PspPPI	RGGWCCY	2 cut(s)	895, 3880
PstI	CTGCAG	4 cut(s)	325, 1837, 3307, 4738
PstNI	CAGNNNCTG	3 cut(s)	2710, 3281, 5608
PsuI	RGATCY	6 cut(s)	604, 1918, 2125, 3589, 4819, 5026
PsyI	GACNNNGTC	1 cut(s)	4997
PvuII	CAGCTG	4 cut(s)	2054, 3161, 4955, 6059
RseI	CAYNNNNRTG	7 cut(s)	504, 623, 1331, 3486, 3608, 4313, 4316
SapI	GCTCTTC	2 cut(s)	438, 3420
Sau96I	GGNCC	8 cut(s)	118, 895, 1296, 1773, 2921, 3880, 4674, 5819
ScaI	AGTACT	1 cut(s)	2745
SchI	GAGTC	6 cut(s)	80, 953, 1164, 3938, 3962, 4149
ScrFI	CCNGG	7 cut(s)	1502, 1916, 2139, 3258, 4817, 5040, 6156
SduI	GDGCHC	4 cut(s)	1356, 4341, 4439, 5644
SfcI	CTRYAG	6 cut(s)	321, 1833, 2769, 3303, 4734, 5667
SinI	GGWCC	7 cut(s)	118, 895, 1773, 2921, 3880, 4674, 5819
SmiMI	CAYNNNNRTG	7 cut(s)	504, 623, 1331, 3486, 3608, 4313, 4316
SmlI	CTYRAG	2 cut(s)	128, 3749
SmoI	CTYRAG	2 cut(s)	128, 3749
SpeI	ACTAGT	2 cut(s)	397, 3379
SphI	GCATGC	3 cut(s)	146, 1042, 4027
SseBI	AGGCCT	2 cut(s)	1415, 4400
SspI	AATATT	1 cut(s)	5500
SspMI	CTAG	8 cut(s)	398, 948, 1089, 2747, 3380, 3933, 4074, 5645
StuI	AGGCCT	2 cut(s)	1415, 4400
StyD4I	CCNGG	7 cut(s)	1500, 1914, 2137, 3256, 4815, 5038, 6154
StyI	CCWWGG	6 cut(s)	1416, 1446, 3106, 4401, 4431, 6004
TaiI	ACGT	7 cut(s)	282, 832, 1608, 1676, 1797, 3817, 4698
TaqII	GACCGA	1 cut(s)	5366
TatI	WGTACW	5 cut(s)	1904, 2743, 3079, 4805, 5977
TauI	GCSGC	5 cut(s)	45, 51, 149, 2815, 5713
TspGWI	ACGGA	7 cut(s)	75, 541, 2291, 2774, 3523, 5192, 5672
Tth111I	GACNNNGTC	1 cut(s)	4997
Vha464I	CTTAAG	1 cut(s)	3749
VpaK11BI	GGWCC	7 cut(s)	118, 895, 1773, 2921, 3880, 4674, 5819
XceI	RCATGY	6 cut(s)	146, 245, 1042, 1440, 4027, 4425
XmiI	GTMKAC	4 cut(s)	826, 2905, 3811, 5803
XmnI	GAANNNNTTC	3 cut(s)	2046, 2598, 4947
XspI	CTAG	8 cut(s)	398, 948, 1089, 2747, 3380, 3933, 4074, 5645
ZrmI	AGTACT	1 cut(s)	2745
Zsp2I	ATGCAT	2 cut(s)	538, 3520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.