Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003103

Physical Location & Seq

Genomic Coordinates Reverse (-)

27154281 .. 27173578

Gene Structure 19298 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1246100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGAATCAATCGAGAGGCTCACGTTCGGATTTGGAGGCCAGCGGAAGCCGCTCCGGTGACTTGGACGGCGAGTCCTCGTCCAGTCCTTTTTACATTGCCAGAACTAAGGATGCTTCGGTCGATCGCCTCAAGCGCTGGAGGCAAGCAGCACTTGTGCTGAATGCTTCTCGTCGATTCCGGTACACCTTAGACTTGAAAAAGGAAGAAGAAAAGCAGCATACACTAAGGAAGATTAGAGCACATGCACAAGCCATACGAGCTGCATATCTTTTCAAGGAAGCTGCAGATCAACAAGTGAATGGGATTGTAGTGTCGAAACCAAGTCCTACTGGCGATTTTCCCATTGGACAAGAGGAACTAGTTCCAGTGACGAGGGATCATAATTTTGCTGCTCTTCAACAATATGGGGGGGTCACAGGCTTGGGGGATTTATTGAAAACAAATCTCGACAAGGGAATTCATGGAGATGATGCTGATTTACTAAAACGGAAAAATGCATTTGGGTCGAACACATATCCTAGGAAAAAAGCAAGAAGTTTCTGGAGATTTCTTTGGGAAGCTTGGCAAGATCTTACTCTGATCATTTTGATGGTAGCTGCAGTGGCTTCTTTAGCGCTGGGTATAAAAACAGAGGGTATAGAGGAGGGATGGTATGATGGGGGCAGCATTGCTTTTGCAGTTCTCCTTGTCATTGTTGTCACAGCCGTTAGTGATTACAGACAATCACTTCAGTTCCAGAATCTAAATGAGGAAAAGAGAAATATACATTTGGAGGTTATTAGAGGTGGTAGACGTGTTGAGGTCTCAATTTATGATCTTGTTGTTGGTGATGTTGTACCCCTTAACATCGGCGATCAGGTCCCTGCTGATGGCATTTTAATAACTGGACACTCACTTGCCATAGATGAGTCTAGTATGACTGGAGAGAGCAAGATTGTGAGCCTCAATACATGTACGCAAGGACTCCAAAATCCATTTCTAATGTCAGGCTGCAAAGTAGCAGATGGAAATGGTAGCATGCTGGTAACCAGTGTTGGGACTAATACTGAATGGGGAATGCTCATGGCTAGTATTTCAGAAGATACTGGTGAGGAAACTCCTTTGCAGGTGCGCTTAAACGGGGTTGCAACTTTCATTGGTATTGTTGGACTCACAGTGGCTTTACTTGTCTTGATAGTCCTGTTGGTTAGATACTTTACTGGCCATACAAAAAATGCAAATGGAAGCCCTCAGTTTAAAGCGGGAAAGACAAAGTTTGGAGATGCCATAGATGGAGCCATTAAAATTGTTACTATTGCGGTCACCATTGTGGTGGTTGCTGTTCCTGAAGGGCTCCCATTAGCTGTTACCTTGACGCTTGCCTACTCGATGAGAAAAATGATGGCAGATAAGGCCTTGGTGCGGAGGCTGTCTGCATGTGAAACCATGGGCTCTGCAACTACCATATGCAGCGACAAGACTGGAACCTTAACATTGAACCAGATGACTGTTGTTGAGGCTTTTACTGGTGGGAGGAAAATCGATCTCTCTGGCAGTAAGTCAGACGTGTCTCCAATACTATCATCTTTGCTTATTGAAGGCATTGCGTTGAATACAAATGGCAGTGTCTATACGCCTGAGACTGGCGGTGATATAGAGGTTTCTGGATCACCAACTGAAAAGGCAATTCTGCAATTGGGAATCAAGCTCGGAATGAATTTTGATGCCATCAGGTCCAAGTCTTCAATTCTTCACGTGTTCCCATTCAACTCAGAGAAAAAACGAGGCGGTGCTGCAGTTAAATTGCCCAATACCGAAGTTCATATTCACTGGAAAGGGGCTGCTGAAATAGTCTTGGCCTGTTGTACAAAGTACCTGGATCCTAATGACCAGTTAGTAGCTATGGATGAAGACAAGTCAACCATGTTCAGGAAGTCAATTGAGGATATGGCTGCTCGCAGTTTGCGTTGTGTTGCCATTGCTTATAGACCACATGAATTGGAAAATGTTCCAGCTGATGAACAACTATCTGAGTGGGCATTGCCTGATGATGACCTTGTCTTGCTTGCTATTGTTGGTATAAAGGATCCTTGTCGGCCTGGGGTGGGAGATGCAGTGCGACTATGCCAAAAAGCTGGTGTTAAGGTACGCATGGTCACAGGTGACAATCTTCAAACTGCGAAAGCGATTGCTATGGAATGTGGCATACTTGCTCCAGATTCAGATGCTAACCAGCCAACTCTCATTGAAGGAACAGCTTTCCGTGAACTTTCAGATAAGCAGAGAGAAGATTGTGCTGAAAAGATTTCGGTGATGGGGCGGTCTTCCCCCAATGACAAGCTTCTGCTTGTGCAAGCATTGAGGAGAAGGGGTCATGTTGTTGCTGTAACTGGAGATGGCACTAATGATGCTCCTGCACTGCACGAGGCTGACATCGGTCTTGCAATGGGTATTCAAGGGACTGAAGTTGCCAAAGAGAGTTCAGATATCATTATCTTGGACGATAATTTTGCTTCAGTTGTGAAGGTTAGTAACTGTGAACATTTTGCAATTTTTCTTAGTAATATCATTGTGTATTGTGTATTGTGTATTGTGTTGTCGCACTCTGTTCTACCTTTCTGTTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

869

Amino Acids

93.7

Weight (kDa)

5.86

Isoelectric Point (pI)

38.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	28 - 70	2.8e-16	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	136 - 203	1.1e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	257 - 357	1e-18	P-type ATPase actuator domain
Hydrolase	PF00702	481 - 804	3.3e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	524 - 693	1.9e-32	P-type ATPase, cytoplasmic domain N

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29900 AT4G29900 AT5G57110 AT5G57110 AT5G57110
fragaria_vesca	FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940
malus_domestica	MD13G1241000.v1.1 MD16G1245600.v1.1 MD16G1246100.v1.1
prunus_persica	Prupe.1G072600_v2.0.a1 Prupe.8G012900_v2.0.a1
pyrus_communis	pycom13g21220
rosa_chinensis	RchiOBHm_Chr4g0398891 RchiOBHm_Chr4g0398951
rosa_laevigata	RLG00000009307 RLG00000009309 RLG00000009365
rosa_multiflora	Rmu_sc0004094.1_g000033 Rmu_ssc0000064.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343340 Rroxscaffold_5G00343930 Rroxscaffold_5G00344000
rosa_rugosa	Rorug04G0013200
rosa_samantha	Rh4AG091100 Rh4CG093100 Rh4CG099100 Rh4CG099400
rosa_wichuraiana	Rw4G007100 Rw4G007130 Rw4G007500 Rw4G007540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1096
Acc36I	ACCTGC	1 cut(s)	1096
AccBSI	CCGCTC	1 cut(s)	51
AccI	GTMKAC	1 cut(s)	790
AciI	CCGC	8 cut(s)	42, 49, 1241, 1298, 1402, 1626, 1767, 2299
AclWI	GGATC	6 cut(s)	384, 1654, 1853, 1866, 2060, 2073
AcoI	YGGCCR	1 cut(s)	1201
AcsI	RAATTY	2 cut(s)	456, 1696
AcuI	CTGAAG	4 cut(s)	713, 1347, 2463, 2478
AcvI	CACGTG	1 cut(s)	1735
AfaI	GTAC	6 cut(s)	182, 837, 955, 1846, 1853, 2127
AfeI	AGCGCT	2 cut(s)	134, 615
AfiI	CCNNNNNNNGG	6 cut(s)	869, 1035, 1401, 1622, 1908, 2084
AflIII	ACRYGT	4 cut(s)	793, 950, 1545, 1734
AhdI	GACNNNNNGTC	1 cut(s)	70
AhlI	ACTAGT	1 cut(s)	358
AjiI	CACGTC	2 cut(s)	794, 1546
AjnI	CCWGG	2 cut(s)	1854, 2077
AjuI	GAANNNNNNNTTGG	2 cut(s)	960, 992
AleI	CACNNNNGTG	1 cut(s)	1307
Alw21I	GWGCWC	1 cut(s)	241
Alw26I	GTCTC	3 cut(s)	808, 1554, 1613
AlwI	GGATC	6 cut(s)	384, 1654, 1853, 1866, 2060, 2073
Aor51HI	AGCGCT	2 cut(s)	134, 615
AoxI	GGCC	5 cut(s)	36, 1201, 1392, 1836, 2075
ApoI	RAATTY	2 cut(s)	456, 1696
ArsI	GACNNNNNNTTYG	2 cut(s)	2471, 2503
Asp700I	GAANNNNTTC	2 cut(s)	360, 1986
AspA2I	CCTAGG	1 cut(s)	518
AspLEI	GCGC	3 cut(s)	135, 616, 1113
AspS9I	GGNCC	2 cut(s)	859, 1713
AsuHPI	GGTGA	8 cut(s)	68, 839, 1100, 1294, 1640, 1641, 2153, 2302
AvaII	GGWCC	2 cut(s)	859, 1713
AvrII	CCTAGG	1 cut(s)	518
BalI	TGGCCA	1 cut(s)	1203
BamHI	GGATCC	2 cut(s)	1858, 2065
BanII	GRGCYC	2 cut(s)	1335, 1433
BauI	CACGAG	1 cut(s)	2402
BbrPI	CACGTG	1 cut(s)	1735
BbsI	GAAGAC	3 cut(s)	1713, 1896, 2295
Bbv12I	GWGCWC	1 cut(s)	241
BceAI	ACGGC	2 cut(s)	82, 689
BcgI	CGANNNNNNTGC	4 cut(s)	486, 520, 2471, 2505
BciT130I	CCWGG	2 cut(s)	1856, 2079
BclI	TGATCA	1 cut(s)	579
BcoDI	GTCTC	3 cut(s)	808, 1554, 1613
BcuI	ACTAGT	1 cut(s)	358
BfaI	CTAG	4 cut(s)	359, 519, 912, 1068
BfmI	CTRYAG	3 cut(s)	282, 597, 1773
BfoI	RGCGCY	2 cut(s)	136, 617
BfuAI	ACCTGC	1 cut(s)	1096
BglII	AGATCT	1 cut(s)	568
BlnI	CCTAGG	1 cut(s)	518
Bme1390I	CCNGG	2 cut(s)	1856, 2079
Bme18I	GGWCC	2 cut(s)	859, 1713
BmeRI	GACNNNNNGTC	1 cut(s)	70
BmgBI	CACGTC	2 cut(s)	794, 1546
BmgT120I	GGNCC	2 cut(s)	859, 1713
BmiI	GGNNCC	6 cut(s)	861, 1276, 1334, 1465, 1860, 2067
BmrFI	CCNGG	2 cut(s)	1856, 2079
BmsI	GCATC	7 cut(s)	100, 460, 1252, 1693, 2080, 2194, 2377
BoxI	GACNNNNGTC	1 cut(s)	2415
BpiI	GAAGAC	3 cut(s)	1713, 1896, 2295
BpmI	CTGGAG	5 cut(s)	157, 562, 942, 2178, 2391
BpuEI	CTTGAG	1 cut(s)	113
Bsa29I	ATCGAT	1 cut(s)	1521
BsaAI	YACGTR	1 cut(s)	1735
BsaI	GGTCTC	1 cut(s)	808
BsaJI	CCNNGG	4 cut(s)	518, 1395, 1425, 2078
BsaWI	WCCGGW	2 cut(s)	53, 177
BsaXI	ACNNNNNCTCC	6 cut(s)	635, 665, 2079, 2109, 2335, 2365
Bsc4I	CCNNNNNNNGG	6 cut(s)	869, 1035, 1401, 1622, 1908, 2084
Bse3DI	GCAATG	6 cut(s)	93, 666, 1581, 1956, 2018, 2430
BseBI	CCWGG	2 cut(s)	1856, 2079
BseCI	ATCGAT	1 cut(s)	1521
BseDI	CCNNGG	4 cut(s)	518, 1395, 1425, 2078
BseGI	GGATG	3 cut(s)	115, 653, 1891
BseLI	CCNNNNNNNGG	6 cut(s)	869, 1035, 1401, 1622, 1908, 2084
BseMI	GCAATG	6 cut(s)	93, 666, 1581, 1956, 2018, 2430
BseMII	CTCAG	4 cut(s)	1244, 1608, 1764, 2001
BseRI	GAGGAG	2 cut(s)	656, 2356
BseYI	CCCAGC	1 cut(s)	616
BsgI	GTGCAG	2 cut(s)	2379, 2384
Bsh1285I	CGRYCG	2 cut(s)	120, 124
BshFI	GGCC	5 cut(s)	38, 1203, 1394, 1838, 2077
BshVI	ATCGAT	1 cut(s)	1521
BsiEI	CGRYCG	2 cut(s)	120, 124
BsiHKAI	GWGCWC	1 cut(s)	241
BsiSI	CCGG	2 cut(s)	54, 178
BslFI	GGGAC	3 cut(s)	845, 1051, 2452
BslI	CCNNNNNNNGG	6 cut(s)	869, 1035, 1401, 1622, 1908, 2084
BsmAI	GTCTC	3 cut(s)	808, 1554, 1613
BsmFI	GGGAC	3 cut(s)	845, 1051, 2452
BsmI	GAATGC	2 cut(s)	166, 1062
BsnI	GGCC	5 cut(s)	38, 1203, 1394, 1838, 2077
Bso31I	GGTCTC	1 cut(s)	808
Bsp1286I	GDGCHC	3 cut(s)	241, 1335, 1433
Bsp1407I	TGTACA	1 cut(s)	1844
Bsp19I	CCATGG	1 cut(s)	1425
BspACI	CCGC	8 cut(s)	42, 49, 1241, 1298, 1402, 1626, 1767, 2299
BspANI	GGCC	5 cut(s)	38, 1203, 1394, 1838, 2077
BspCNI	CTCAG	4 cut(s)	1243, 1609, 1763, 2002
BspDI	ATCGAT	1 cut(s)	1521
BspLI	GGNNCC	6 cut(s)	861, 1276, 1334, 1465, 1860, 2067
BspMAI	CTGCAG	3 cut(s)	286, 601, 1777
BspMI	ACCTGC	1 cut(s)	1096
BspPI	GGATC	6 cut(s)	384, 1654, 1853, 1866, 2060, 2073
BspQI	GCTCTTC	1 cut(s)	399
BspTNI	GGTCTC	1 cut(s)	808
BsrBI	CCGCTC	1 cut(s)	51
BsrDI	GCAATG	6 cut(s)	93, 666, 1581, 1956, 2018, 2430
BsrGI	TGTACA	1 cut(s)	1844
BssECI	CCNNGG	4 cut(s)	518, 1395, 1425, 2078
BssSI	CACGAG	1 cut(s)	2402
BssT1I	CCWWGG	3 cut(s)	518, 1395, 1425
Bst2BI	CACGAG	1 cut(s)	2402
Bst2UI	CCWGG	2 cut(s)	1856, 2079
Bst4CI	ACNGT	3 cut(s)	1156, 1489, 2516
Bst6I	CTCTTC	1 cut(s)	399
BstAUI	TGTACA	1 cut(s)	1844
BstBAI	YACGTR	1 cut(s)	1735
BstC8I	GCNNGC	7 cut(s)	40, 144, 1019, 1359, 1936, 2046, 2334
BstDEI	CTNAG	8 cut(s)	105, 187, 224, 1230, 1617, 1750, 2010, 2537
BstDSI	CCRYGG	1 cut(s)	1425
BstEII	GGTNACC	2 cut(s)	1024, 1300
BstF5I	GGATG	3 cut(s)	115, 653, 1891
BstH2I	RGCGCY	2 cut(s)	136, 617
BstHHI	GCGC	3 cut(s)	135, 616, 1113
BstMAI	GTCTC	3 cut(s)	808, 1554, 1613
BstMCI	CGRYCG	2 cut(s)	120, 124
BstMWI	GCNNNNNNNGC	7 cut(s)	48, 132, 139, 257, 602, 611, 1391
BstNI	CCWGG	2 cut(s)	1856, 2079
BstNSI	RCATGY	4 cut(s)	245, 954, 1021, 1419
BstPAI	GACNNNNGTC	1 cut(s)	2415
BstPI	GGTNACC	2 cut(s)	1024, 1300
BstSCI	CCNGG	2 cut(s)	1854, 2077
BstSFI	CTRYAG	3 cut(s)	282, 597, 1773
BstV2I	GAAGAC	3 cut(s)	1713, 1896, 2295
BstX2I	RGATCY	3 cut(s)	568, 1858, 2065
BstXI	CCANNNNNNTGG	2 cut(s)	1312, 2114
BstYI	RGATCY	3 cut(s)	568, 1858, 2065
Bsu15I	ATCGAT	1 cut(s)	1521
BsuRI	GGCC	5 cut(s)	38, 1203, 1394, 1838, 2077
BsuTUI	ATCGAT	1 cut(s)	1521
BtgI	CCRYGG	1 cut(s)	1425
BtrI	CACGTC	2 cut(s)	794, 1546
BtsCI	GGATG	3 cut(s)	115, 653, 1891
BtsI	GCAGTG	4 cut(s)	606, 1609, 2100, 2396
BtsIMutI	CAGTG	8 cut(s)	372, 606, 1036, 1161, 1609, 1807, 2100, 2396
BveI	ACCTGC	1 cut(s)	1096
Cac8I	GCNNGC	7 cut(s)	40, 144, 1019, 1359, 1936, 2046, 2334
CfoI	GCGC	3 cut(s)	135, 616, 1113
Cfr13I	GGNCC	2 cut(s)	859, 1713
ClaI	ATCGAT	1 cut(s)	1521
CseI	GACGC	1 cut(s)	1363
Csp6I	GTAC	6 cut(s)	181, 836, 954, 1845, 1852, 2126
CspCI	CAANNNNNGTGG	2 cut(s)	1959, 1994
CviQI	GTAC	6 cut(s)	181, 836, 954, 1845, 1852, 2126
DdeI	CTNAG	8 cut(s)	105, 187, 224, 1230, 1617, 1750, 2010, 2537
DraI	TTTAAA	1 cut(s)	1237
DriI	GACNNNNNGTC	1 cut(s)	70
EaeI	YGGCCR	1 cut(s)	1201
Eam1104I	CTCTTC	1 cut(s)	399
Eam1105I	GACNNNNNGTC	1 cut(s)	70
EarI	CTCTTC	1 cut(s)	399
Eco130I	CCWWGG	3 cut(s)	518, 1395, 1425
Eco147I	AGGCCT	1 cut(s)	1394
Eco24I	GRGCYC	2 cut(s)	1335, 1433
Eco31I	GGTCTC	1 cut(s)	808
Eco32I	GATATC	1 cut(s)	2467
Eco47I	GGWCC	2 cut(s)	859, 1713
Eco47III	AGCGCT	2 cut(s)	134, 615
Eco57I	CTGAAG	4 cut(s)	713, 1347, 2463, 2478
Eco72I	CACGTG	1 cut(s)	1735
Eco91I	GGTNACC	2 cut(s)	1024, 1300
EcoO109I	RGGNCCY	1 cut(s)	859
EcoO65I	GGTNACC	2 cut(s)	1024, 1300
EcoRI	GAATTC	1 cut(s)	456
EcoRII	CCWGG	2 cut(s)	1854, 2077
EcoRV	GATATC	1 cut(s)	2467
EcoT14I	CCWWGG	3 cut(s)	518, 1395, 1425
EcoT22I	ATGCAT	1 cut(s)	499
EcoT38I	GRGCYC	2 cut(s)	1335, 1433
ErhI	CCWWGG	3 cut(s)	518, 1395, 1425
FalI	AAGNNNNNCTT	2 cut(s)	135, 167
FaqI	GGGAC	3 cut(s)	845, 1051, 2452
FauI	CCCGC	1 cut(s)	1234
FauNDI	CATATG	1 cut(s)	1445
FbaI	TGATCA	1 cut(s)	579
FblI	GTMKAC	1 cut(s)	790
FokI	GGATG	3 cut(s)	122, 660, 1898
FriOI	GRGCYC	2 cut(s)	1335, 1433
FspBI	CTAG	4 cut(s)	359, 519, 912, 1068
GlaI	GCGC	3 cut(s)	134, 615, 1112
GsaI	CCCAGC	1 cut(s)	620
GsuI	CTGGAG	5 cut(s)	157, 562, 942, 2178, 2391
HaeII	RGCGCY	2 cut(s)	136, 617
HaeIII	GGCC	5 cut(s)	38, 1203, 1394, 1838, 2077
HapII	CCGG	2 cut(s)	54, 178
HgaI	GACGC	1 cut(s)	1363
HhaI	GCGC	3 cut(s)	135, 616, 1113
Hin6I	GCGC	3 cut(s)	133, 614, 1111
HinP1I	GCGC	3 cut(s)	133, 614, 1111
HincII	GTYRAC	1 cut(s)	1899
HindII	GTYRAC	1 cut(s)	1899
HindIII	AAGCTT	2 cut(s)	558, 2318
HinfI	GANTC	9 cut(s)	4, 71, 174, 739, 908, 963, 1149, 1680, 2198
HpaII	CCGG	2 cut(s)	54, 178
HphI	GGTGA	8 cut(s)	68, 839, 1100, 1294, 1640, 1641, 2153, 2302
Hpy166II	GTNNAC	5 cut(s)	183, 791, 1899, 2246, 2519
Hpy188III	TCNNGA	9 cut(s)	12, 446, 541, 736, 1171, 1325, 1644, 1909, 2195
Hpy8I	GTNNAC	5 cut(s)	183, 791, 1899, 2246, 2519
Hpy99I	CGWCG	1 cut(s)	174
HpyAV	CCTTC	5 cut(s)	1322, 1571, 2222, 2340, 2497
HpyCH4III	ACNGT	3 cut(s)	1156, 1489, 2516
HpyCH4IV	ACGT	4 cut(s)	22, 793, 1545, 1734
HpyF10VI	GCNNNNNNNGC	7 cut(s)	48, 132, 139, 257, 602, 611, 1391
HpyF3I	CTNAG	8 cut(s)	105, 187, 224, 1230, 1617, 1750, 2010, 2537
HpySE526I	ACGT	4 cut(s)	22, 793, 1545, 1734
HspAI	GCGC	3 cut(s)	133, 614, 1111
Ksp22I	TGATCA	1 cut(s)	579
LguI	GCTCTTC	1 cut(s)	399
LmnI	GCTCC	5 cut(s)	56, 1274, 1338, 2197, 2395
LweI	GCATC	7 cut(s)	100, 460, 1252, 1693, 2080, 2194, 2377
MaeI	CTAG	4 cut(s)	359, 519, 912, 1068
MaeII	ACGT	4 cut(s)	22, 793, 1545, 1734
MbiI	CCGCTC	1 cut(s)	51
MfeI	CAATTG	2 cut(s)	1673, 1917
MflI	RGATCY	3 cut(s)	568, 1858, 2065
MhlI	GDGCHC	3 cut(s)	241, 1335, 1433
MlsI	TGGCCA	1 cut(s)	1203
MluNI	TGGCCA	1 cut(s)	1203
MlyI	GAGTC	4 cut(s)	80, 917, 957, 1143
MmeI	TCCRAC	1 cut(s)	1126
Mox20I	TGGCCA	1 cut(s)	1203
Mph1103I	ATGCAT	1 cut(s)	499
MroXI	GAANNNNTTC	2 cut(s)	360, 1986
MscI	TGGCCA	1 cut(s)	1203
MseI	TTAA	8 cut(s)	843, 878, 1115, 1236, 1281, 1469, 1779, 2121
MslI	CAYNNNNRTG	4 cut(s)	465, 587, 1307, 1310
Msp20I	TGGCCA	1 cut(s)	1203
MspA1I	CMGCKG	2 cut(s)	42, 1994
MspI	CCGG	2 cut(s)	54, 178
MspR9I	CCNGG	2 cut(s)	1856, 2079
MunI	CAATTG	2 cut(s)	1673, 1917
Mva1269I	GAATGC	2 cut(s)	166, 1062
MvaI	CCWGG	2 cut(s)	1856, 2079
MwoI	GCNNNNNNNGC	7 cut(s)	48, 132, 139, 257, 602, 611, 1391
NcoI	CCATGG	1 cut(s)	1425
NdeI	CATATG	1 cut(s)	1445
NlaIV	GGNNCC	6 cut(s)	861, 1276, 1334, 1465, 1860, 2067
NmuCI	GTSAC	7 cut(s)	56, 367, 412, 697, 1300, 2134, 2141
NsiI	ATGCAT	1 cut(s)	499
NspI	RCATGY	4 cut(s)	245, 954, 1021, 1419
OliI	CACNNNNGTG	1 cut(s)	1307
PaeI	GCATGC	1 cut(s)	1021
PaqCI	CACCTGC	1 cut(s)	1096
PceI	AGGCCT	1 cut(s)	1394
PciI	ACATGT	1 cut(s)	950
PciSI	GCTCTTC	1 cut(s)	399
PcsI	WCGNNNNNNNCGW	1 cut(s)	1942
PctI	GAATGC	2 cut(s)	166, 1062
PdmI	GAANNNNTTC	2 cut(s)	360, 1986
PfeI	GAWTC	5 cut(s)	4, 174, 739, 1680, 2198
PflFI	GACNNNGTC	1 cut(s)	2036
Ple19I	CGATCG	1 cut(s)	124
PleI	GAGTC	4 cut(s)	79, 916, 957, 1143
PmaCI	CACGTG	1 cut(s)	1735
PmlI	CACGTG	1 cut(s)	1735
PpsI	GAGTC	4 cut(s)	79, 916, 957, 1143
Ppu21I	YACGTR	1 cut(s)	1735
PpuMI	RGGWCCY	1 cut(s)	859
PscI	ACATGT	1 cut(s)	950
PshAI	GACNNNNGTC	1 cut(s)	2415
Psp5II	RGGWCCY	1 cut(s)	859
Psp6I	CCWGG	2 cut(s)	1854, 2077
PspCI	CACGTG	1 cut(s)	1735
PspEI	GGTNACC	2 cut(s)	1024, 1300
PspFI	CCCAGC	1 cut(s)	616
PspGI	CCWGG	2 cut(s)	1854, 2077
PspN4I	GGNNCC	6 cut(s)	861, 1276, 1334, 1465, 1860, 2067
PspPI	GGNCC	2 cut(s)	859, 1713
PspPPI	RGGWCCY	1 cut(s)	859
PstI	CTGCAG	3 cut(s)	286, 601, 1777
PsuI	RGATCY	3 cut(s)	568, 1858, 2065
PsyI	GACNNNGTC	1 cut(s)	2036
PvuI	CGATCG	1 cut(s)	124
PvuII	CAGCTG	1 cut(s)	1994
RsaI	GTAC	6 cut(s)	182, 837, 955, 1846, 1853, 2127
RsaNI	GTAC	6 cut(s)	181, 836, 954, 1845, 1852, 2126
RseI	CAYNNNNRTG	4 cut(s)	465, 587, 1307, 1310
SapI	GCTCTTC	1 cut(s)	399
SaqAI	TTAA	8 cut(s)	843, 878, 1115, 1236, 1281, 1469, 1779, 2121
Sau96I	GGNCC	2 cut(s)	859, 1713
SchI	GAGTC	4 cut(s)	80, 917, 957, 1143
ScrFI	CCNGG	2 cut(s)	1856, 2079
SduI	GDGCHC	3 cut(s)	241, 1335, 1433
SfaNI	GCATC	7 cut(s)	100, 460, 1252, 1693, 2080, 2194, 2377
SfcI	CTRYAG	3 cut(s)	282, 597, 1773
SinI	GGWCC	2 cut(s)	859, 1713
SmiMI	CAYNNNNRTG	4 cut(s)	465, 587, 1307, 1310
SmlI	CTYRAG	1 cut(s)	128
SmoI	CTYRAG	1 cut(s)	128
SpeI	ACTAGT	1 cut(s)	358
SphI	GCATGC	1 cut(s)	1021
SseBI	AGGCCT	1 cut(s)	1394
SsiI	CCGC	8 cut(s)	42, 49, 1241, 1298, 1402, 1626, 1767, 2299
SspMI	CTAG	4 cut(s)	359, 519, 912, 1068
StuI	AGGCCT	1 cut(s)	1394
StyD4I	CCNGG	2 cut(s)	1854, 2077
StyI	CCWWGG	3 cut(s)	518, 1395, 1425
TaaI	ACNGT	3 cut(s)	1156, 1489, 2516
TaiI	ACGT	4 cut(s)	25, 796, 1548, 1737
TaqI	TCGA	8 cut(s)	11, 120, 172, 314, 447, 506, 1367, 1521
TaqII	GACCGA	2 cut(s)	106, 2405
TatI	WGTACW	1 cut(s)	1844
TauI	GCSGC	1 cut(s)	51
TfiI	GAWTC	5 cut(s)	4, 174, 739, 1680, 2198
Tru1I	TTAA	8 cut(s)	843, 878, 1115, 1236, 1281, 1469, 1779, 2121
Tru9I	TTAA	8 cut(s)	843, 878, 1115, 1236, 1281, 1469, 1779, 2121
TscAI	CASTG	8 cut(s)	372, 606, 1036, 1161, 1609, 1814, 2100, 2403
TseFI	GTSAC	7 cut(s)	56, 367, 412, 697, 1300, 2134, 2141
Tsp45I	GTSAC	7 cut(s)	56, 367, 412, 697, 1300, 2134, 2141
TspDTI	ATGAA	9 cut(s)	17, 449, 1123, 1709, 1790, 1902, 1989, 2013, 2592
TspGWI	ACGGA	2 cut(s)	502, 2231
TspRI	CASTG	8 cut(s)	372, 606, 1036, 1161, 1609, 1814, 2100, 2403
Tth111I	GACNNNGTC	1 cut(s)	2036
VpaK11BI	GGWCC	2 cut(s)	859, 1713
XapI	RAATTY	2 cut(s)	456, 1696
XceI	RCATGY	4 cut(s)	245, 954, 1021, 1419
XmaJI	CCTAGG	1 cut(s)	518
XmiI	GTMKAC	1 cut(s)	790
XmnI	GAANNNNTTC	2 cut(s)	360, 1986
XspI	CTAG	4 cut(s)	359, 519, 912, 1068
Zsp2I	ATGCAT	1 cut(s)	499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.