Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003103

Physical Location & Seq

Genomic Coordinates Forward (+)

14852903 .. 14881778

Gene Structure 28876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G007100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3225 bp

ATGCATTTGGTTCAGATTAGCTATCTCAATCCTAGGCAACAAGTCTTCGGGTCCCTACAATCACCTTATACTTTCTTGACAGATTCTAAAAGTCATATGCTTTCTTTGTTTTTTTTATTGCAGCTTGGTCACCCAATAAGCAGCTCGCCTGACCGACCAAACCACGATTTAGAGGCCGGAACCAGTCGCTCAGGCAACGTGAACGACGATGAGTCGTGGACCAATCCTTTTGATATGAACAGTACCAAGTATGCTTCTGTTGAACGCCTCAAGCGTTGGAGGAAAGCAGCACTTGTACTGAATTCTTCTCGCCGATTCCGATACACCCTCAACTTGAAAAAGGAAGAAGAGAAGAAACAGATGCTGAGGAAGATAAGAGCACATGCACAAGCCATACGAGCTGCATCTTTATACGCAGAAGTTGGAAATCCCTTGCAAGGAACACAACCGATACCATCCGCTGCTGGCGATTTTCAGATTGCCCATGAGCAACTTGTTTCAGTGATCAGGGACCGTGACTTACATGCTATGCAGCAATATGGGGGAGTCAGTGGCTTAGCAGATTTATTGAAAACCAATCTACACAAGGGAATTCTTGGTGATAGTGCTGATTGTCTAAACCGGAAAAATGCATTTGGATCCAACACATATCCTCCTGGAAGAGCAAGGAGCTTCTTGACTATTCTTTGGGAAGCCTGGCAAGACCTTACTCTGATCATTTTGATGACAGCCGCAGTGGCTACCTTAGGGCTACATATAAATATGAAGGATATGAAGGAGGAATGGTATGGTGGTGGCAGCATTGCTTTAGCAGTTATTCTTGTCATTGTTGTCATAGCCATTAGTGATTACAATGAATCCCTGCGCTTCCAGAGTTTGATTGCAGAGAGGAGAAATATACATTTAGAGGTCATCAGAGGTGGTAGACGAGTTGAGGTTTCTATCTATGATATTGTTGTTGGTGATGTTGTACCGCTTAGCATTGGTGATCAGGTCCCGGCTGATGGTATTTTAATCACTGGCTACTCACTTGCTACAGAGGAGACCCAAATCATTGGGGATAGCAAGATTGTTCACAAGGACTGCAAGGACCCATTTCTGATGTCTGGCTGCAAAATTGCATATGGGAGTGGTACAATGCTGGTAACTAGCGTAGGGATGAATACTGAATGGGGTTTGCTCATGGCTCGTATTTCAGAAGATATTGGTGAAGAAACACCTGTGCAGGTGGGTTTAAATGGAGTTGCTACTGTCATTGGAGTTGTTGGGGTCGTAATAGCTCTTTTCGTCTTCATTTTCGATTTAGTTAGATTATTTACTGGCCATTCAAGCAATACAAATGGAACTCTTCAGTTTGTAGCCGGCAAGACTAAATTTGATAGAGCCATTCAACTTAGTTTCATTATAGTGACCAATGTAATGGTTGCTGTACCTGAAGGGCTCCCTATTGCTGTCATGTTAATAAATGATGCTGTTAACCTCTCATTATGCCTGCAATTCTTATTTACTTATCAGAAAGCACATGATTTCATGGTATATTCATCTTTATGTGCCTCTCATTTCTCTGTCCGTTATGACATCCTTGCCTATTCCATGACCAAAATGATGGCAGATAAGGTCTTGGTGCGGAGGCAATCTGCATGCGAGGCTATAGGCTCTGCCACAACTATTTGCATTGATAAGACTGGACTTTTAACTTCGAACCAGATGACTGTTGTTGAGGCTTATGTTGGTGGGAAGAAAATCTATCCTTCAGACAATAAGTCGCTATTGTCTCCAGTGCTATGGTCCTTAATTATTGAAAGCATTGCACAGAACACAGATGGCAGTGTCTACATGGCTGAGAATGGTGGTGATGTAGAGGTTTATGGATCACCAACTGAAAAAGCAATTCTGCGCTGGGGGATTAAGATTGGAATGAATTTTGAAGCCACGAAGTCTGAATCTACAGTTCTTCATGCGCTCGCATTTAACTCGGTGAAGAAACGAAGTGCTGTTGCACTTAAATTGGCCAATTCTCAAGTTCATATACACTGGAAAGGGGCTGCTGAAACAATCTTGGCCTCATGCACAAAGTACATTGATGCTAATGATCACTTGGTAGCAATGGATGAAGATAAGTTAATGTTTTTCAATGAAGCTATTGAGGGTATGGCTTCTGGAAGTCTGCGGTGTGTTGCTATTGCATATAGGTTATACGAGTTTGAAAATGTTCCGACTGATGAACAACTAGTTCGGTGGGCAATACCTGAGGATGACCTTATTTTACTTGCTATTGTTGGCATAGAGGATCCTTGTCAGCCAGGAGCCAGAGATGCAGTGCGACAATGCCAAAATGCTGGTGTTAAGGTATGCATGGTTACGGGTGACAATCTTCAAACAGCAAAAGCAATTGCTTTGGAGTGTGGCATACTTGGTTCCGAGAGTATTTCGTGCCTTTCTGATAAGCAAAGAGAAGATTTAGCTGAGAGGTATCAGTATGATCCGAATATCAAATTATCCATCTCTCATCTCAATTACTCACTGAAGAAAAGGGGACATGTTGTTGCTGTCACTGGTGATGGTTGTAATGATGCTCCGGCATTATATGAGCTAAAAGAGAGTTCAGATATAATCATCTTGGATAATAACTTTTCTACAATTGTGAAGGCTATCAAATGGGGTCGATCTGTATATTCAAATATTCAGAAATTTACCCAGTTCCATCTTACAGTTGGACTTGCAGCATTAATCATAAATGTCATTGCAGAAGTTTCCTCTGGTTCAGCTCCATTAAATGCTGTGCAGATTCTCTGGGTAAGCATCATCATGGACACTCAAGGAGCACTAGTATTGCGTACTGATCCACCATCTGATAACCTGATGCGGAAGCCTCCTTCTTTCTACCAAGTCGCTATCTTGCTTATTCTAAATTTCCACGGAAGGAATTTGCTGAATTTGGAACTTGCTGATTACAATATTTTCAATGAATTCAATGCTCGAAAGCCCGAGGAATATAACATTTTCAAAGGAATTACTTCAAACTATCTCTTTCTGGGAATAGTGGGAATAACTCTTGTGCTTCAGGTTTGGCCCCTTGATGCTATCGGGAAACTCTTACCAGTTCCAAAAACTCCCTTCATCAAGTTTGCCTTGAAGCTTTGGGGCGATCGCTTTTCCAGCATCTTTTGGACACTGTGCCGCCTTAGCCAAGCATGCTCGCTCAGCCTTGCAGGTAGAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1074

Amino Acids

118.38

Weight (kDa)

6.45

Isoelectric Point (pI)

39.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	75 - 117	2.2e-15	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	180 - 246	2e-10	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	303 - 396	2.9e-15	P-type ATPase actuator domain
Hydrolase	PF00702	556 - 862	3e-10	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	607 - 768	1.3e-27	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	923 - 960	2e-08	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29900 AT4G29900 AT5G57110 AT5G57110 AT5G57110
fragaria_vesca	FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940
malus_domestica	MD13G1241000.v1.1 MD16G1245600.v1.1 MD16G1246100.v1.1
prunus_persica	Prupe.1G072600_v2.0.a1 Prupe.8G012900_v2.0.a1
pyrus_communis	pycom13g21220
rosa_chinensis	RchiOBHm_Chr4g0398891 RchiOBHm_Chr4g0398951
rosa_laevigata	RLG00000009307 RLG00000009309 RLG00000009365
rosa_multiflora	Rmu_sc0004094.1_g000033 Rmu_ssc0000064.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343340 Rroxscaffold_5G00343930 Rroxscaffold_5G00344000
rosa_rugosa	Rorug04G0013200
rosa_samantha	Rh4AG091100 Rh4CG093100 Rh4CG099100 Rh4CG099400
rosa_wichuraiana	Rw4G007100 Rw4G007130 Rw4G007500 Rw4G007540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1216
Acc36I	ACCTGC	2 cut(s)	1216, 3203
AccI	GTMKAC	2 cut(s)	925, 1833
AciI	CCGC	7 cut(s)	459, 732, 974, 1627, 2170, 2866, 3181
AclWI	GGATC	7 cut(s)	633, 646, 1879, 2285, 2298, 2477, 2837
AcoI	YGGCCR	2 cut(s)	1321, 2010
AcsI	RAATTY	9 cut(s)	301, 591, 1373, 1921, 2690, 2911, 2926, 2935, 2969
AcuI	CTGAAG	5 cut(s)	1334, 1455, 1737, 2546, 3047
AfaI	GTAC	7 cut(s)	244, 297, 972, 1135, 1431, 2078, 2839
AfiI	CCNNNNNNNGG	2 cut(s)	437, 1004
AflIII	ACRYGT	1 cut(s)	2539
AhdI	GACNNNNNGTC	2 cut(s)	211, 1762
AhlI	ACTAGT	2 cut(s)	2230, 2827
AjnI	CCWGG	3 cut(s)	655, 695, 2302
Alw21I	GWGCWC	2 cut(s)	382, 2827
Alw26I	GTCTC	2 cut(s)	1037, 1779
AlwI	GGATC	7 cut(s)	633, 646, 1879, 2285, 2298, 2477, 2837
AlwNI	CAGNNNCTG	1 cut(s)	364
Ama87I	CYCGRG	1 cut(s)	2987
AoxI	GGCC	5 cut(s)	174, 1321, 2010, 2061, 3071
ApoI	RAATTY	9 cut(s)	301, 591, 1373, 1921, 2690, 2911, 2926, 2935, 2969
AseI	ATTAAT	1 cut(s)	2729
AsiSI	GCGATCGC	1 cut(s)	3151
Asp700I	GAANNNNTTC	2 cut(s)	2211, 3016
AspA2I	CCTAGG	1 cut(s)	32
AspLEI	GCGC	3 cut(s)	867, 1899, 1963
AspS9I	GGNCC	7 cut(s)	51, 219, 511, 994, 1090, 1788, 3072
AsuC2I	CCSGG	1 cut(s)	998
AsuII	TTCGAA	1 cut(s)	1700
AvaI	CYCGRG	1 cut(s)	2987
AvaII	GGWCC	6 cut(s)	51, 219, 511, 994, 1090, 1788
AvrII	CCTAGG	1 cut(s)	32
AxyI	CCTNAGG	2 cut(s)	745, 2250
BalI	TGGCCA	2 cut(s)	1323, 2012
BamHI	GGATCC	2 cut(s)	638, 2290
BanII	GRGCYC	1 cut(s)	1443
BbsI	GAAGAC	2 cut(s)	37, 1282
Bbv12I	GWGCWC	2 cut(s)	382, 2827
BbvCI	CCTCAGC	1 cut(s)	365
BccI	CCATC	8 cut(s)	463, 998, 1600, 1817, 2510, 2555, 2712, 2857
BcgI	CGANNNNNNTGC	2 cut(s)	1956, 1990
BciT130I	CCWGG	3 cut(s)	657, 697, 2304
BclI	TGATCA	4 cut(s)	504, 714, 988, 2092
BcnI	CCSGG	1 cut(s)	998
BcoDI	GTCTC	2 cut(s)	1037, 1779
BcuI	ACTAGT	2 cut(s)	2230, 2827
BfaI	CTAG	4 cut(s)	33, 1149, 2231, 2828
BfmI	CTRYAG	3 cut(s)	1035, 1650, 1947
BfuAI	ACCTGC	2 cut(s)	1216, 3203
BglI	GCCNNNNNGGC	1 cut(s)	737
BlnI	CCTAGG	1 cut(s)	32
BlpI	GCTNAGC	3 cut(s)	556, 977, 3203
Bme1390I	CCNGG	4 cut(s)	657, 697, 998, 2304
Bme18I	GGWCC	6 cut(s)	51, 219, 511, 994, 1090, 1788
BmeRI	GACNNNNNGTC	2 cut(s)	211, 1762
BmeT110I	CYCGRG	1 cut(s)	2987
BmgT120I	GGNCC	7 cut(s)	51, 219, 511, 994, 1090, 1788, 3072
BmrFI	CCNGG	4 cut(s)	657, 697, 998, 2304
BmrI	ACTGGG	1 cut(s)	2692
BmuI	ACTGGG	1 cut(s)	2692
BpiI	GAAGAC	2 cut(s)	37, 1282
BpmI	CTGGAG	1 cut(s)	1761
Bpu10I	CCTNAGC	3 cut(s)	190, 365, 3185
Bpu1102I	GCTNAGC	3 cut(s)	556, 977, 3203
Bpu14I	TTCGAA	1 cut(s)	1700
BpuEI	CTTGAG	3 cut(s)	254, 2004, 2802
BpuMI	CCSGG	1 cut(s)	998
BsaI	GGTCTC	1 cut(s)	1037
BsaJI	CCNNGG	3 cut(s)	32, 2917, 2988
BsaWI	WCCGGW	1 cut(s)	621
BsaXI	ACNNNNNCTCC	6 cut(s)	637, 667, 770, 800, 2814, 2844
Bsc4I	CCNNNNNNNGG	2 cut(s)	437, 1004
Bse118I	RCCGGY	1 cut(s)	1361
Bse1I	ACTGG	9 cut(s)	183, 1024, 1324, 1690, 1778, 2039, 2560, 2698, 3101
Bse21I	CCTNAGG	2 cut(s)	745, 2250
Bse3DI	GCAATG	4 cut(s)	801, 1806, 2112, 2742
BseBI	CCWGG	3 cut(s)	657, 697, 2304
BseDI	CCNNGG	3 cut(s)	32, 2917, 2988
BseGI	GGATG	5 cut(s)	455, 1164, 1578, 2116, 2260
BseLI	CCNNNNNNNGG	2 cut(s)	437, 1004
BseMI	GCAATG	4 cut(s)	801, 1806, 2112, 2742
BseMII	CTCAG	6 cut(s)	204, 356, 1833, 2241, 2457, 3217
BseNI	ACTGG	9 cut(s)	183, 1024, 1324, 1690, 1778, 2039, 2560, 2698, 3101
BseRI	GAGGAG	2 cut(s)	904, 1055
BseYI	CCCAGC	1 cut(s)	1899
BsgI	GTGCAG	2 cut(s)	1244, 2804
Bsh1285I	CGRYCG	1 cut(s)	3151
BshFI	GGCC	5 cut(s)	176, 1323, 2012, 2063, 3073
BsiEI	CGRYCG	1 cut(s)	3151
BsiHKAI	GWGCWC	2 cut(s)	382, 2827
BsiHKCI	CYCGRG	1 cut(s)	2987
BsiSI	CCGG	5 cut(s)	177, 622, 998, 1362, 2579
BslFI	GGGAC	4 cut(s)	37, 524, 980, 2550
BslI	CCNNNNNNNGG	2 cut(s)	437, 1004
BsmAI	GTCTC	2 cut(s)	1037, 1779
BsmFI	GGGAC	4 cut(s)	37, 524, 980, 2550
BsnI	GGCC	5 cut(s)	176, 1323, 2012, 2063, 3073
Bso31I	GGTCTC	1 cut(s)	1037
BsoBI	CYCGRG	1 cut(s)	2987
Bsp119I	TTCGAA	1 cut(s)	1700
Bsp1286I	GDGCHC	3 cut(s)	382, 1443, 2827
Bsp1720I	GCTNAGC	3 cut(s)	556, 977, 3203
BspACI	CCGC	7 cut(s)	459, 732, 974, 1627, 2170, 2866, 3181
BspANI	GGCC	5 cut(s)	176, 1323, 2012, 2063, 3073
BspCNI	CTCAG	6 cut(s)	203, 357, 1834, 2242, 2458, 3216
BspMI	ACCTGC	2 cut(s)	1216, 3203
BspPI	GGATC	7 cut(s)	633, 646, 1879, 2285, 2298, 2477, 2837
BspQI	GCTCTTC	1 cut(s)	655
BspT104I	TTCGAA	1 cut(s)	1700
BspTNI	GGTCTC	1 cut(s)	1037
BsrDI	GCAATG	4 cut(s)	801, 1806, 2112, 2742
BsrFI	RCCGGY	1 cut(s)	1361
BsrI	ACTGG	9 cut(s)	183, 1024, 1324, 1690, 1778, 2039, 2560, 2698, 3101
BssAI	RCCGGY	1 cut(s)	1361
BssECI	CCNNGG	3 cut(s)	32, 2917, 2988
BssT1I	CCWWGG	1 cut(s)	32
Bst2UI	CCWGG	3 cut(s)	657, 697, 2304
Bst4CI	ACNGT	7 cut(s)	242, 515, 1252, 1714, 1951, 2713, 3177
Bst6I	CTCTTC	3 cut(s)	342, 655, 1353
BstBI	TTCGAA	1 cut(s)	1700
BstC8I	GCNNGC	8 cut(s)	146, 466, 1363, 1493, 1642, 1965, 3196, 3200
BstDSI	CCRYGG	1 cut(s)	2917
BstEII	GGTNACC	1 cut(s)	128
BstF5I	GGATG	5 cut(s)	455, 1164, 1578, 2116, 2260
BstHHI	GCGC	3 cut(s)	867, 1899, 1963
BstMAI	GTCTC	2 cut(s)	1037, 1779
BstMCI	CGRYCG	1 cut(s)	3151
BstMWI	GCNNNNNNNGC	9 cut(s)	398, 737, 1329, 1646, 2315, 3159, 3186, 3195, 3204
BstNI	CCWGG	3 cut(s)	657, 697, 2304
BstNSI	RCATGY	5 cut(s)	386, 527, 1644, 2543, 3198
BstPI	GGTNACC	1 cut(s)	128
BstSCI	CCNGG	4 cut(s)	655, 695, 996, 2302
BstSFI	CTRYAG	3 cut(s)	1035, 1650, 1947
BstV2I	GAAGAC	2 cut(s)	37, 1282
BstX2I	RGATCY	2 cut(s)	638, 2290
BstXI	CCANNNNNNTGG	5 cut(s)	1055, 1420, 1606, 1785, 2339
BstYI	RGATCY	2 cut(s)	638, 2290
Bsu36I	CCTNAGG	2 cut(s)	745, 2250
BsuRI	GGCC	5 cut(s)	176, 1323, 2012, 2063, 3073
BtgI	CCRYGG	1 cut(s)	2917
BtsCI	GGATG	5 cut(s)	455, 1164, 1578, 2116, 2260
BtsI	GCAGTG	3 cut(s)	741, 1834, 2325
BveI	ACCTGC	2 cut(s)	1216, 3203
Cac8I	GCNNGC	8 cut(s)	146, 466, 1363, 1493, 1642, 1965, 3196, 3200
CaiI	CAGNNNCTG	1 cut(s)	364
CfoI	GCGC	3 cut(s)	867, 1899, 1963
Cfr10I	RCCGGY	1 cut(s)	1361
Cfr13I	GGNCC	7 cut(s)	51, 219, 511, 994, 1090, 1788, 3072
Csp6I	GTAC	7 cut(s)	243, 296, 971, 1134, 1430, 2077, 2838
CviQI	GTAC	7 cut(s)	243, 296, 971, 1134, 1430, 2077, 2838
DraI	TTTAAA	1 cut(s)	1236
DriI	GACNNNNNGTC	2 cut(s)	211, 1762
EaeI	YGGCCR	2 cut(s)	1321, 2010
Eam1104I	CTCTTC	3 cut(s)	342, 655, 1353
Eam1105I	GACNNNNNGTC	2 cut(s)	211, 1762
EarI	CTCTTC	3 cut(s)	342, 655, 1353
Eco130I	CCWWGG	1 cut(s)	32
Eco24I	GRGCYC	1 cut(s)	1443
Eco31I	GGTCTC	1 cut(s)	1037
Eco47I	GGWCC	6 cut(s)	51, 219, 511, 994, 1090, 1788
Eco57I	CTGAAG	5 cut(s)	1334, 1455, 1737, 2546, 3047
Eco81I	CCTNAGG	2 cut(s)	745, 2250
Eco88I	CYCGRG	1 cut(s)	2987
Eco91I	GGTNACC	1 cut(s)	128
EcoO109I	RGGNCCY	3 cut(s)	51, 994, 1090
EcoO65I	GGTNACC	1 cut(s)	128
EcoRI	GAATTC	3 cut(s)	301, 591, 2969
EcoRII	CCWGG	3 cut(s)	655, 695, 2302
EcoT14I	CCWWGG	1 cut(s)	32
EcoT22I	ATGCAT	3 cut(s)	6, 634, 2357
EcoT38I	GRGCYC	1 cut(s)	1443
ErhI	CCWWGG	1 cut(s)	32
FalI	AAGNNNNNCTT	4 cut(s)	276, 308, 3116, 3148
FaqI	GGGAC	4 cut(s)	37, 524, 980, 2550
FauNDI	CATATG	2 cut(s)	96, 1123
FbaI	TGATCA	4 cut(s)	504, 714, 988, 2092
FblI	GTMKAC	2 cut(s)	925, 1833
FokI	GGATG	5 cut(s)	442, 1171, 1565, 2123, 2267
FriOI	GRGCYC	1 cut(s)	1443
FspBI	CTAG	4 cut(s)	33, 1149, 2231, 2828
GlaI	GCGC	3 cut(s)	866, 1898, 1962
GsaI	CCCAGC	1 cut(s)	1903
GsuI	CTGGAG	1 cut(s)	1761
HaeIII	GGCC	5 cut(s)	176, 1323, 2012, 2063, 3073
HapII	CCGG	5 cut(s)	177, 622, 998, 1362, 2579
HhaI	GCGC	3 cut(s)	867, 1899, 1963
Hin6I	GCGC	3 cut(s)	865, 1897, 1961
HinP1I	GCGC	3 cut(s)	865, 1897, 1961
HincII	GTYRAC	1 cut(s)	1477
HindII	GTYRAC	1 cut(s)	1477
HindIII	AAGCTT	1 cut(s)	3137
HinfI	GANTC	7 cut(s)	83, 212, 315, 546, 857, 1943, 2788
HpaI	GTTAAC	1 cut(s)	1477
HpaII	CCGG	5 cut(s)	177, 622, 998, 1362, 2579
Hpy166II	GTNNAC	6 cut(s)	202, 219, 926, 1075, 1477, 1834
Hpy188III	TCNNGA	5 cut(s)	76, 676, 871, 2160, 3088
Hpy8I	GTNNAC	6 cut(s)	202, 219, 926, 1075, 1477, 1834
Hpy99I	CGWCG	1 cut(s)	209
HpyAV	CCTTC	8 cut(s)	760, 769, 1430, 1761, 2641, 2886, 2916, 3127
HpyCH4III	ACNGT	7 cut(s)	242, 515, 1252, 1714, 1951, 2713, 3177
HpyCH4IV	ACGT	1 cut(s)	198
HpyF10VI	GCNNNNNNNGC	9 cut(s)	398, 737, 1329, 1646, 2315, 3159, 3186, 3195, 3204
HpySE526I	ACGT	1 cut(s)	198
HspAI	GCGC	3 cut(s)	865, 1897, 1961
KflI	GGGWCCC	1 cut(s)	51
KroI	GCCGGC	1 cut(s)	1361
KroNI	GCCGGC	1 cut(s)	1363
Ksp22I	TGATCA	4 cut(s)	504, 714, 988, 2092
KspAI	GTTAAC	1 cut(s)	1477
LguI	GCTCTTC	1 cut(s)	655
LmnI	GCTCC	6 cut(s)	669, 1446, 2306, 2581, 2773, 2822
MaeI	CTAG	4 cut(s)	33, 1149, 2231, 2828
MaeII	ACGT	1 cut(s)	198
MaeIII	GTNAC	7 cut(s)	128, 515, 1144, 1408, 2359, 2366, 2551
MfeI	CAATTG	2 cut(s)	2391, 2640
MflI	RGATCY	2 cut(s)	638, 2290
MhlI	GDGCHC	3 cut(s)	382, 1443, 2827
MlsI	TGGCCA	2 cut(s)	1323, 2012
MluNI	TGGCCA	2 cut(s)	1323, 2012
MlyI	GAGTC	2 cut(s)	221, 555
MmeI	TCCRAC	5 cut(s)	257, 403, 666, 2240, 2695
Mox20I	TGGCCA	2 cut(s)	1323, 2012
Mph1103I	ATGCAT	3 cut(s)	6, 634, 2357
MroNI	GCCGGC	1 cut(s)	1361
MroXI	GAANNNNTTC	2 cut(s)	2211, 3016
MscI	TGGCCA	2 cut(s)	1323, 2012
MslI	CAYNNNNRTG	4 cut(s)	722, 1406, 1546, 2807
Msp20I	TGGCCA	2 cut(s)	1323, 2012
MspA1I	CMGCKG	1 cut(s)	461
MspI	CCGG	5 cut(s)	177, 622, 998, 1362, 2579
MspR9I	CCNGG	4 cut(s)	657, 697, 998, 2304
MunI	CAATTG	2 cut(s)	2391, 2640
MvaI	CCWGG	3 cut(s)	657, 697, 2304
MwoI	GCNNNNNNNGC	9 cut(s)	398, 737, 1329, 1646, 2315, 3159, 3186, 3195, 3204
NaeI	GCCGGC	1 cut(s)	1363
NciI	CCSGG	1 cut(s)	998
NdeI	CATATG	2 cut(s)	96, 1123
NgoMIV	GCCGGC	1 cut(s)	1361
NmuCI	GTSAC	5 cut(s)	128, 515, 1408, 2366, 2551
NsiI	ATGCAT	3 cut(s)	6, 634, 2357
NspI	RCATGY	5 cut(s)	386, 527, 1644, 2543, 3198
NspV	TTCGAA	1 cut(s)	1700
PaeI	GCATGC	2 cut(s)	1644, 3198
PaqCI	CACCTGC	1 cut(s)	1216
PciI	ACATGT	1 cut(s)	2539
PciSI	GCTCTTC	1 cut(s)	655
PcsI	WCGNNNNNNNCGW	3 cut(s)	204, 271, 316
PdiI	GCCGGC	1 cut(s)	1363
PdmI	GAANNNNTTC	2 cut(s)	2211, 3016
PfeI	GAWTC	5 cut(s)	83, 315, 857, 1943, 2788
PfoI	TCCNGGA	1 cut(s)	655
Ple19I	CGATCG	1 cut(s)	3151
PleI	GAGTC	2 cut(s)	220, 554
PpsI	GAGTC	2 cut(s)	220, 554
PpuMI	RGGWCCY	3 cut(s)	51, 994, 1090
PscI	ACATGT	1 cut(s)	2539
PshBI	ATTAAT	1 cut(s)	2729
Psp5II	RGGWCCY	3 cut(s)	51, 994, 1090
Psp6I	CCWGG	3 cut(s)	655, 695, 2302
PspEI	GGTNACC	1 cut(s)	128
PspFI	CCCAGC	1 cut(s)	1899
PspGI	CCWGG	3 cut(s)	655, 695, 2302
PspPI	GGNCC	7 cut(s)	51, 219, 511, 994, 1090, 1788, 3072
PspPPI	RGGWCCY	3 cut(s)	51, 994, 1090
PstNI	CAGNNNCTG	1 cut(s)	364
PsuI	RGATCY	2 cut(s)	638, 2290
PvuI	CGATCG	1 cut(s)	3151
RgaI	GCGATCGC	1 cut(s)	3151
RsaI	GTAC	7 cut(s)	244, 297, 972, 1135, 1431, 2078, 2839
RsaNI	GTAC	7 cut(s)	243, 296, 971, 1134, 1430, 2077, 2838
RseI	CAYNNNNRTG	4 cut(s)	722, 1406, 1546, 2807
SapI	GCTCTTC	1 cut(s)	655
Sau96I	GGNCC	7 cut(s)	51, 219, 511, 994, 1090, 1788, 3072
SchI	GAGTC	2 cut(s)	221, 555
ScrFI	CCNGG	4 cut(s)	657, 697, 998, 2304
SduI	GDGCHC	3 cut(s)	382, 1443, 2827
SfaAI	GCGATCGC	1 cut(s)	3151
SfcI	CTRYAG	3 cut(s)	1035, 1650, 1947
SfuI	TTCGAA	1 cut(s)	1700
SgfI	GCGATCGC	1 cut(s)	3151
SinI	GGWCC	6 cut(s)	51, 219, 511, 994, 1090, 1788
SmiMI	CAYNNNNRTG	4 cut(s)	722, 1406, 1546, 2807
SmlI	CTYRAG	3 cut(s)	269, 2019, 2817
SmoI	CTYRAG	3 cut(s)	269, 2019, 2817
SpeI	ACTAGT	2 cut(s)	2230, 2827
SphI	GCATGC	2 cut(s)	1644, 3198
SsiI	CCGC	7 cut(s)	459, 732, 974, 1627, 2170, 2866, 3181
SspI	AATATT	2 cut(s)	2683, 2959
SspMI	CTAG	4 cut(s)	33, 1149, 2231, 2828
StyD4I	CCNGG	4 cut(s)	655, 695, 996, 2302
StyI	CCWWGG	1 cut(s)	32
TaaI	ACNGT	7 cut(s)	242, 515, 1252, 1714, 1951, 2713, 3177
TaiI	ACGT	1 cut(s)	201
TaqI	TCGA	4 cut(s)	1299, 1700, 2665, 2980
TaqII	GACCGA	1 cut(s)	168
TatI	WGTACW	2 cut(s)	295, 2076
TauI	GCSGC	2 cut(s)	734, 3183
TfiI	GAWTC	5 cut(s)	83, 315, 857, 1943, 2788
TseFI	GTSAC	5 cut(s)	128, 515, 1408, 2366, 2551
Tsp45I	GTSAC	5 cut(s)	128, 515, 1408, 2366, 2551
TspGWI	ACGGA	2 cut(s)	1559, 2934
VpaK11BI	GGWCC	6 cut(s)	51, 219, 511, 994, 1090, 1788
VspI	ATTAAT	1 cut(s)	2729
XapI	RAATTY	9 cut(s)	301, 591, 1373, 1921, 2690, 2911, 2926, 2935, 2969
XceI	RCATGY	5 cut(s)	386, 527, 1644, 2543, 3198
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2711
XmaJI	CCTAGG	1 cut(s)	32
XmiI	GTMKAC	2 cut(s)	925, 1833
XmnI	GAANNNNTTC	2 cut(s)	2211, 3016
XspI	CTAG	4 cut(s)	33, 1149, 2231, 2828
Zsp2I	ATGCAT	3 cut(s)	6, 634, 2357

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.