MD14G1035700.v1.1

transcription repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004746
Physical Location & Seq
Reverse (-)
3222098 .. 3222646
549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1035700.v1.1.491

Sequence Viewer

Length: 549 bp
ATGCCTACAACAACCAGAAGAAAGCTCCGGCTGAGCAAAGTCACAGTCGACATAGGGTGCGGCATCTGCAGAAAACCCAGTAAGCTCTTCCAAATGTTCAACCCTAAACCTAAACCCTCCTCCAAAACCCTAAAAAACAACCACTTTCCGCCTCCTCCTTCTTCTTCTTCAACCCCACGCAACAATTACAACAAACTCTACAAAGACAACGACCACGCTCCTTTTTTAGTCACAGATTCCGACACGAAGAGCACGAGACCAGTCAACGGATTCGGTAGAATCGGCAACGAGAGCGTGGCCGTGGAGAAAGACTCCGGCGACCCTTACTTGGATTTTCGACACTCGATGCTACAGATGATACTGGAGAACGAGATATACTCCAAAGACGATCTCCGGGAGCTTCTCAACTGTTTCCTGCAGCTCAACTCTCCCTGCAACCACGGCATTATCATCAGGGTCTTCACTGAGATCTGGAATGCTGTTTTCTCTGTCAGGTCTGGAGGTTCGACGTTGCTGCACTTTGCGAGCAACAAGTCATGCGACTACTAG

Protein Analysis

183

Amino Acids

20.6

Weight (kDa)

9.36

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 105 - 161 9.2e-24 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AciI CCGC 2 cut(s) 60, 149
AcoI YGGCCR 1 cut(s) 297
AfiI CCNNNNNNNGG 2 cut(s) 266, 328
AgsI TTSAA 2 cut(s) 100, 171
AluBI AGCT 4 cut(s) 25, 85, 400, 421
AluI AGCT 4 cut(s) 25, 85, 400, 421
Alw21I GWGCWC 1 cut(s) 254
Alw26I GTCTC 1 cut(s) 250
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 418, 514
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AsuC2I CCSGG 1 cut(s) 395
BauI CACGAG 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 254
BbvI GCAGC 2 cut(s) 430, 501
BceAI ACGGC 2 cut(s) 284, 457
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 250
BfaI CTAG 1 cut(s) 547
BfmI CTRYAG 3 cut(s) 67, 350, 416
BglII AGATCT 1 cut(s) 468
BisI GCNGC 3 cut(s) 61, 419, 515
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 3 cut(s) 62, 420, 516
Bme1390I CCNGG 1 cut(s) 395
BmrFI CCNGG 1 cut(s) 395
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 2 cut(s) 72, 336
BmuI ACTGGG 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 451
BplI GAGNNNNNCTC 4 cut(s) 296, 328, 362, 394
BpmI CTGGAG 2 cut(s) 383, 519
Bpu1102I GCTNAGC 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 395
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 300, 439
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 328
Bse1I ACTGG 3 cut(s) 78, 260, 366
BseDI CCNNGG 2 cut(s) 300, 439
BseLI CCNNNNNNNGG 2 cut(s) 266, 328
BseMII CTCAG 2 cut(s) 23, 456
BseNI ACTGG 3 cut(s) 78, 260, 366
BseRI GAGGAG 2 cut(s) 109, 144
BseXI GCAGC 2 cut(s) 430, 501
BsgI GTGCAG 1 cut(s) 500
BshFI GGCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 254
BsiSI CCGG 3 cut(s) 28, 315, 394
BslI CCNNNNNNNGG 2 cut(s) 266, 328
BsmAI GTCTC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 1 cut(s) 299
Bso31I GGTCTC 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 2 cut(s) 388, 468
Bsp1720I GCTNAGC 1 cut(s) 32
BspACI CCGC 2 cut(s) 60, 149
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 2 cut(s) 24, 457
BspMAI CTGCAG 2 cut(s) 71, 420
BspQI GCTCTTC 2 cut(s) 92, 242
BspTNI GGTCTC 1 cut(s) 250
BsrI ACTGG 3 cut(s) 78, 260, 366
BssECI CCNNGG 2 cut(s) 300, 439
BssMI GATC 2 cut(s) 388, 468
BssSI CACGAG 1 cut(s) 253
Bst2BI CACGAG 1 cut(s) 253
Bst4CI ACNGT 2 cut(s) 46, 410
Bst6I CTCTTC 2 cut(s) 92, 242
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 2 cut(s) 32, 465
BstDSI CCRYGG 2 cut(s) 300, 439
BstKTI GATC 2 cut(s) 391, 471
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 2 cut(s) 388, 468
BstMWI GCNNNNNNNGC 3 cut(s) 66, 291, 441
BstSCI CCNGG 1 cut(s) 393
BstSFI CTRYAG 3 cut(s) 67, 350, 416
BstV1I GCAGC 2 cut(s) 430, 501
BstV2I GAAGAC 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 468
BstYI RGATCY 1 cut(s) 468
BsuRI GGCC 1 cut(s) 299
BtgI CCRYGG 2 cut(s) 300, 439
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 1 cut(s) 526
CviAII CATG 1 cut(s) 537
CviJI RGCY 6 cut(s) 25, 31, 85, 299, 400, 421
CviKI_1 RGCY 6 cut(s) 25, 31, 85, 299, 400, 421
DdeI CTNAG 2 cut(s) 32, 465
DpnI GATC 2 cut(s) 390, 470
DpnII GATC 2 cut(s) 388, 468
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 92, 242
EarI CTCTTC 2 cut(s) 92, 242
EciI GGCGGA 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 250
FaeI CATG 1 cut(s) 540
FaiI YATR 3 cut(s) 53, 376, 538
FatI CATG 1 cut(s) 536
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 61, 419, 515
Fsp4HI GCNGC 3 cut(s) 61, 419, 515
FspBI CTAG 1 cut(s) 547
GluI GCNGC 3 cut(s) 61, 419, 515
GsuI CTGGAG 2 cut(s) 383, 519
HaeIII GGCC 1 cut(s) 299
HapII CCGG 3 cut(s) 28, 315, 394
Hin1II CATG 1 cut(s) 540
HincII GTYRAC 2 cut(s) 49, 265
HindII GTYRAC 2 cut(s) 49, 265
HinfI GANTC 4 cut(s) 236, 270, 279, 311
HpaII CCGG 3 cut(s) 28, 315, 394
Hpy166II GTNNAC 2 cut(s) 49, 265
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 2 cut(s) 472, 498
Hpy8I GTNNAC 2 cut(s) 49, 265
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 1 cut(s) 168
HpyCH4III ACNGT 2 cut(s) 46, 410
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 4 cut(s) 69, 418, 435, 517
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 291, 441
HpyF3I CTNAG 2 cut(s) 32, 465
HpySE526I ACGT 1 cut(s) 509
Hsp92II CATG 1 cut(s) 540
Kzo9I GATC 2 cut(s) 388, 468
LguI GCTCTTC 2 cut(s) 92, 242
LmnI GCTCC 3 cut(s) 30, 223, 397
Lsp1109I GCAGC 2 cut(s) 430, 501
LweI GCATC 2 cut(s) 72, 336
MaeI CTAG 1 cut(s) 547
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 2 cut(s) 40, 229
MalI GATC 2 cut(s) 390, 470
MboI GATC 2 cut(s) 388, 468
MboII GAAGA 7 cut(s) 30, 79, 153, 156, 159, 259, 451
MflI RGATCY 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 1 cut(s) 184
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 5 cut(s) 127, 130, 162, 165, 494
MspI CCGG 3 cut(s) 28, 315, 394
MspR9I CCNGG 1 cut(s) 395
Mva1269I GAATGC 1 cut(s) 481
MwoI GCNNNNNNNGC 3 cut(s) 66, 291, 441
NciI CCSGG 1 cut(s) 395
NdeII GATC 2 cut(s) 388, 468
NlaIII CATG 1 cut(s) 540
NmuCI GTSAC 2 cut(s) 40, 229
PciSI GCTCTTC 2 cut(s) 92, 242
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PctI GAATGC 1 cut(s) 481
PfeI GAWTC 3 cut(s) 236, 270, 279
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 3 cut(s) 62, 420, 516
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
PsrI GAACNNNNNNTAC 2 cut(s) 359, 391
PstI CTGCAG 2 cut(s) 71, 420
PsuI RGATCY 1 cut(s) 468
SalI GTCGAC 1 cut(s) 47
SapI GCTCTTC 2 cut(s) 92, 242
SatI GCNGC 3 cut(s) 61, 419, 515
Sau3AI GATC 2 cut(s) 388, 468
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 395
SduI GDGCHC 1 cut(s) 254
SetI ASST 8 cut(s) 27, 87, 112, 402, 423, 497, 505, 512
SfaNI GCATC 2 cut(s) 72, 336
SfcI CTRYAG 3 cut(s) 67, 350, 416
Sse9I AATT 1 cut(s) 184
SsiI CCGC 2 cut(s) 60, 149
SspMI CTAG 1 cut(s) 547
StyD4I CCNGG 1 cut(s) 393
TaaI ACNGT 2 cut(s) 46, 410
TaiI ACGT 1 cut(s) 512
TaqI TCGA 4 cut(s) 48, 337, 344, 506
TasI AATT 1 cut(s) 184
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 3 cut(s) 236, 270, 279
TscAI CASTG 1 cut(s) 469
TseFI GTSAC 2 cut(s) 40, 229
TseI GCWGC 2 cut(s) 418, 514
Tsp45I GTSAC 2 cut(s) 40, 229
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.