Rroxscaffold_6G00406400

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004746
Physical Location & Seq
Forward (+)
28862037 .. 28862594
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00406400.1

Sequence Viewer

Length: 558 bp
ATGCCTACCATAACCAGAAAGAAGCTCCTTAACACTGTGACAGCCGTTGACATAGGCTGCAGCAGCTGCAGAAGACCAAAGCTCTTCCACATATTCCGTCCCAAGGCAAAGCCAATCACCACCAAGTTCGGCGGCCGCAAACAAAACCACCACCTCTACTCTTCTTCGAGCTCCTACAACAAAGACTCCACCACCAGCACATTCTCCCCAAACACCGACGACTACGCTTCTTTTTCGGACCACGAGACGGTCGCGAAGAGCGCCACGCCCGTCAAGGGGTTCGGCAGGGTTGGCGGCGAGAGCGTGGCGGTGGAGAAGGACTCCGGCGACCCTTACTTGGACTTTAGGCATTCCATGCTTCAGATGATTTTGGAGAACGAGATATACTCTAAAGATGATCTCCGGGAGCTTCTCAACTGCTTCTTGCAGCTCAACGCGCCTTGCAACCATGGCATCATCATCAGGGCTTTCACTGACATCTGGAATGGAGTCTTCTCCGTTAGGTCTAGTGCTTCCACAATGCAGCTTCGTCTTGCTCGCCAGTCACGTGACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ovate

Protein Analysis

185

Amino Acids

20.8

Weight (kDa)

9.34

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 108 - 164 4.1e-24 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 254, 437
AciI CCGC 4 cut(s) 132, 136, 294, 308
AcoI YGGCCR 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 344
AcvI CACGTG 1 cut(s) 548
AfiI CCNNNNNNNGG 5 cut(s) 103, 247, 275, 276, 337
AluBI AGCT 7 cut(s) 25, 66, 82, 171, 409, 430, 526
AluI AGCT 7 cut(s) 25, 66, 82, 171, 409, 430, 526
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 239
AlwNI CAGNNNCTG 1 cut(s) 66
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 6 cut(s) 57, 60, 63, 66, 427, 523
AspLEI GCGC 2 cut(s) 263, 439
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 173
BauI CACGAG 1 cut(s) 242
BbrPI CACGTG 1 cut(s) 548
BbsI GAAGAC 2 cut(s) 79, 484
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 6 cut(s) 44, 53, 72, 75, 439, 535
BceAI ACGGC 1 cut(s) 29
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 2 cut(s) 58, 67
BfoI RGCGCY 1 cut(s) 264
BisI GCNGC 9 cut(s) 58, 61, 64, 67, 133, 136, 295, 428, 524
BlsI GCNGC 9 cut(s) 59, 62, 65, 68, 134, 137, 296, 429, 525
Bme1390I CCNGG 1 cut(s) 404
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 404
BmsI GCATC 1 cut(s) 462
BpiI GAAGAC 2 cut(s) 79, 484
BplI GAGNNNNNCTC 4 cut(s) 305, 337, 371, 403
BpuMI CCSGG 1 cut(s) 404
BsaAI YACGTR 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 102, 448
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 247, 275, 276, 337
Bse1I ACTGG 1 cut(s) 541
BseDI CCNNGG 2 cut(s) 102, 448
BseLI CCNNNNNNNGG 5 cut(s) 103, 247, 275, 276, 337
BseNI ACTGG 1 cut(s) 541
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 6 cut(s) 44, 53, 72, 75, 439, 535
Bsh1236I CGCG 2 cut(s) 254, 437
Bsh1285I CGRYCG 2 cut(s) 136, 252
BshFI GGCC 1 cut(s) 135
BsiEI CGRYCG 2 cut(s) 136, 252
BsiHKAI GWGCWC 1 cut(s) 173
BsiSI CCGG 2 cut(s) 324, 403
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 5 cut(s) 103, 247, 275, 276, 337
BsmAI GTCTC 1 cut(s) 239
BsmBI CGTCTC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 1 cut(s) 397
Bsp19I CCATGG 1 cut(s) 448
Bsp68I TCGCGA 1 cut(s) 254
BspACI CCGC 4 cut(s) 132, 136, 294, 308
BspANI GGCC 1 cut(s) 135
BspFNI CGCG 2 cut(s) 254, 437
BspMAI CTGCAG 2 cut(s) 62, 71
BspQI GCTCTTC 2 cut(s) 89, 251
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 2 cut(s) 102, 448
BssMI GATC 1 cut(s) 397
BssSI CACGAG 1 cut(s) 242
BssT1I CCWWGG 2 cut(s) 102, 448
Bst2BI CACGAG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 37, 250
Bst6I CTCTTC 3 cut(s) 89, 166, 251
BstAPI GCANNNNNTGC 2 cut(s) 66, 355
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 1 cut(s) 538
BstDSI CCRYGG 1 cut(s) 448
BstFNI CGCG 2 cut(s) 254, 437
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 2 cut(s) 263, 439
BstKTI GATC 1 cut(s) 400
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 1 cut(s) 397
BstMCI CGRYCG 2 cut(s) 136, 252
BstMWI GCNNNNNNNGC 8 cut(s) 63, 66, 260, 291, 300, 355, 436, 450
BstSCI CCNGG 1 cut(s) 402
BstSFI CTRYAG 2 cut(s) 58, 67
BstUI CGCG 2 cut(s) 254, 437
BstV1I GCAGC 6 cut(s) 44, 53, 72, 75, 439, 535
BstV2I GAAGAC 2 cut(s) 79, 484
BstZI CGGCCG 1 cut(s) 133
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 448
BtsIMutI CAGTG 2 cut(s) 33, 471
BtuMI TCGCGA 1 cut(s) 254
Cac8I GCNNGC 1 cut(s) 538
CaiI CAGNNNCTG 1 cut(s) 66
CciNI GCGGCCGC 1 cut(s) 133
CfoI GCGC 2 cut(s) 263, 439
Cfr13I GGNCC 1 cut(s) 238
CviAII CATG 2 cut(s) 355, 449
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 133
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 3 cut(s) 89, 166, 251
EarI CTCTTC 3 cut(s) 89, 166, 251
Ecl136II GAGCTC 1 cut(s) 171
EclXI CGGCCG 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 102, 448
Eco24I GRGCYC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 238
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 344
Eco72I CACGTG 1 cut(s) 548
EcoICRI GAGCTC 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 102, 448
EcoT38I GRGCYC 1 cut(s) 173
ErhI CCWWGG 2 cut(s) 102, 448
Esp3I CGTCTC 1 cut(s) 239
FaeI CATG 2 cut(s) 358, 452
FaiI YATR 6 cut(s) 11, 53, 92, 356, 385, 450
FaqI GGGAC 1 cut(s) 84
FatI CATG 2 cut(s) 354, 448
Fnu4HI GCNGC 9 cut(s) 58, 61, 64, 67, 133, 136, 295, 428, 524
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 9 cut(s) 58, 61, 64, 67, 133, 136, 295, 428, 524
FspBI CTAG 1 cut(s) 507
GlaI GCGC 2 cut(s) 262, 438
GluI GCNGC 9 cut(s) 58, 61, 64, 67, 133, 136, 295, 428, 524
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 1 cut(s) 135
HapII CCGG 2 cut(s) 324, 403
HhaI GCGC 2 cut(s) 263, 439
Hin1II CATG 2 cut(s) 358, 452
Hin6I GCGC 2 cut(s) 261, 437
HinP1I GCGC 2 cut(s) 261, 437
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 3 cut(s) 185, 320, 489
HpaII CCGG 2 cut(s) 324, 403
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 2 cut(s) 238, 363
Hpy188III TCNNGA 2 cut(s) 253, 481
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 2 cut(s) 37, 250
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 5 cut(s) 60, 69, 427, 444, 523
HpyF10VI GCNNNNNNNGC 8 cut(s) 63, 66, 260, 291, 300, 355, 436, 450
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 2 cut(s) 358, 452
HspAI GCGC 2 cut(s) 261, 437
Kzo9I GATC 1 cut(s) 397
LguI GCTCTTC 2 cut(s) 89, 251
LmnI GCTCC 3 cut(s) 30, 176, 406
LpnPI CCDG 8 cut(s) 28, 208, 271, 337, 416, 448, 466, 554
Lsp1109I GCAGC 6 cut(s) 44, 53, 72, 75, 439, 535
LweI GCATC 1 cut(s) 462
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 3 cut(s) 37, 543, 548
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 6 cut(s) 76, 84, 153, 156, 268, 484
MhlI GDGCHC 1 cut(s) 173
MlyI GAGTC 3 cut(s) 179, 314, 498
MnlI CCTC 1 cut(s) 164
MseI TTAA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 2 cut(s) 324, 403
MspR9I CCNGG 1 cut(s) 404
Mva1269I GAATGC 1 cut(s) 349
MvnI CGCG 2 cut(s) 254, 437
MwoI GCNNNNNNNGC 8 cut(s) 63, 66, 260, 291, 300, 355, 436, 450
NciI CCSGG 1 cut(s) 404
NcoI CCATGG 1 cut(s) 448
NdeII GATC 1 cut(s) 397
NlaIII CATG 2 cut(s) 358, 452
NmuCI GTSAC 3 cut(s) 37, 543, 548
NotI GCGGCCGC 1 cut(s) 133
NruI TCGCGA 1 cut(s) 254
PciSI GCTCTTC 2 cut(s) 89, 251
PcsI WCGNNNNNNNCGW 1 cut(s) 544
PctI GAATGC 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 9 cut(s) 59, 62, 65, 68, 134, 137, 296, 429, 525
PleI GAGTC 3 cut(s) 179, 314, 497
PmaCI CACGTG 1 cut(s) 548
PmlI CACGTG 1 cut(s) 548
PpsI GAGTC 3 cut(s) 179, 314, 497
Ppu21I YACGTR 1 cut(s) 548
Psp124BI GAGCTC 1 cut(s) 173
PspCI CACGTG 1 cut(s) 548
PspPI GGNCC 1 cut(s) 238
PsrI GAACNNNNNNTAC 2 cut(s) 368, 400
PstI CTGCAG 2 cut(s) 62, 71
PstNI CAGNNNCTG 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 66
RruI TCGCGA 1 cut(s) 254
SacI GAGCTC 1 cut(s) 173
SapI GCTCTTC 2 cut(s) 89, 251
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 9 cut(s) 58, 61, 64, 67, 133, 136, 295, 428, 524
Sau3AI GATC 1 cut(s) 397
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 3 cut(s) 179, 314, 498
ScrFI CCNGG 1 cut(s) 404
SduI GDGCHC 1 cut(s) 173
SfaNI GCATC 1 cut(s) 462
SfcI CTRYAG 2 cut(s) 58, 67
SinI GGWCC 1 cut(s) 238
SsiI CCGC 4 cut(s) 132, 136, 294, 308
SspMI CTAG 1 cut(s) 507
SstI GAGCTC 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 402
StyI CCWWGG 2 cut(s) 102, 448
TaaI ACNGT 2 cut(s) 37, 250
TaiI ACGT 1 cut(s) 550
TaqI TCGA 1 cut(s) 167
TauI GCSGC 3 cut(s) 135, 138, 297
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 2 cut(s) 40, 478
TseFI GTSAC 3 cut(s) 37, 543, 548
TseI GCWGC 6 cut(s) 57, 60, 63, 66, 427, 523
Tsp45I GTSAC 3 cut(s) 37, 543, 548
TspGWI ACGGA 2 cut(s) 86, 487
TspRI CASTG 2 cut(s) 40, 478
VpaK11BI GGWCC 1 cut(s) 238
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.