MD14G1086500.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003398
Physical Location & Seq
Forward (+)
10706285 .. 10710628
4344 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1086500.v1.1.491

Sequence Viewer

Length: 762 bp
ATGTTAGCCTTGTCTCCTCCTATGTTTTCAACCATTGGATGGCCCATAGATCAAGATCCTACAAGCCACGATTACTACAAGGACCATATTACCACTACTAATGATCAAACTGCAGAGTCATCCTCCCTTCGTATACTTCCATCTGGTCGCCCACAGTCTGAGCTTGATGTTTCCACACCATCCACAACCATAAGAGGCGAGTGCTCCAGCGTTTCCCCGGTGGCTAAGAAGCTTAACCATAATGCTAGTGAGCGTGATCGTCGCAAGAAGATCAACAGCTTGTATTCCTCATTGCGTTCATTGCTTCCTGCGGATCAACCGTTACAGAAAAAGTTGAGCATTCCGAATACAGTTTCGCGTGTGGTGAAATACGTCCCGGAGCTCCAAAAGCAAGTGGAGGGATTAATTCGAAAGAGGGAGGAGCTTTTGTCAAGAATAACTAAGCAAGAAAGTGCATTGCATGAGGAGAAGAACCAAATAAAAAGTGCAGCTCGGAGCTCCTTATCTGCTGTTTCAGCATGTCAGCTTAATGACAGAGAAGTAGCAATTCAAATTTCCTCCTTGAAGACCAAAAACAATTTATTATCTGATATCTTGCTAAATTTAGAGGAGGAGGGGCTTCAAATCCTAAATGCTTCTTCCTTCGAGTCGTCTGGAGGGAGGGTTTTCTATAATTTACAATTTCAGGTGGAAAGAACTTACAGACTAGAATGTGAGAGTTTAAGTGACAAGCTCATGTCCTTCTATGGACAAGAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0006357 GO:0006873 GO:0006875 GO:0006879 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010104 GO:0010106 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0019219 GO:0019222 GO:0019725 GO:0023051 GO:0030003 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034248 GO:0034250 GO:0042221 GO:0042493 GO:0042592 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0043565 GO:0044212 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0046685 GO:0046686 GO:0046916 GO:0048518 GO:0048522 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051171 GO:0051173 GO:0051193 GO:0051194 GO:0051252 GO:0051254 GO:0051716 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0060255 GO:0065007 GO:0065008 GO:0070297 GO:0071496 GO:0071731 GO:0080090 GO:0090575 GO:0097159 GO:0097366 GO:0098771 GO:0140110 GO:1900376 GO:1900378 GO:1900704 GO:1900706 GO:1901363 GO:1901698 GO:1901700 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990641 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

254

Amino Acids

28.48

Weight (kDa)

7.69

Isoelectric Point (pI)

67.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 76 - 129 1.2e-13 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 39
AccI GTMKAC 1 cut(s) 133
AccII CGCG 1 cut(s) 358
AciI CCGC 1 cut(s) 311
AclWI GGATC 2 cut(s) 50, 321
AcsI RAATTY 2 cut(s) 552, 601
AfiI CCNNNNNNNGG 1 cut(s) 39
AgsI TTSAA 4 cut(s) 30, 551, 565, 623
AluBI AGCT 9 cut(s) 163, 232, 279, 382, 424, 491, 498, 526, 733
AluI AGCT 9 cut(s) 163, 232, 279, 382, 424, 491, 498, 526, 733
Alw21I GWGCWC 3 cut(s) 206, 384, 500
Alw26I GTCTC 1 cut(s) 18
AlwI GGATC 2 cut(s) 50, 321
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 2 cut(s) 552, 601
AseI ATTAAT 1 cut(s) 404
AspS9I GGNCC 2 cut(s) 42, 82
AsuC2I CCSGG 2 cut(s) 218, 377
AsuHPI GGTGA 1 cut(s) 376
AsuII TTCGAA 1 cut(s) 409
AvaII GGWCC 1 cut(s) 82
BanII GRGCYC 2 cut(s) 384, 500
BbsI GAAGAC 1 cut(s) 572
Bbv12I GWGCWC 3 cut(s) 206, 384, 500
BbvI GCAGC 1 cut(s) 500
BccI CCATC 3 cut(s) 33, 148, 187
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 2 cut(s) 218, 377
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 2 cut(s) 246, 707
BfmI CTRYAG 1 cut(s) 111
BisI GCNGC 1 cut(s) 489
BlsI GCNGC 1 cut(s) 490
Bme1390I CCNGG 2 cut(s) 218, 377
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 42, 82
BmrFI CCNGG 2 cut(s) 218, 377
BpiI GAAGAC 1 cut(s) 572
BplI GAGNNNNNCTC 2 cut(s) 107, 139
BpmI CTGGAG 2 cut(s) 190, 675
Bpu14I TTCGAA 1 cut(s) 409
BpuMI CCSGG 2 cut(s) 218, 377
BsaBI GATNNNNATC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 216
BsaXI ACNNNNNCTCC 4 cut(s) 413, 443, 648, 678
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse3DI GCAATG 3 cut(s) 290, 299, 455
Bse8I GATNNNNATC 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 3 cut(s) 44, 119, 179
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 39
BseMI GCAATG 3 cut(s) 290, 299, 455
BseMII CTCAG 1 cut(s) 150
BseRI GAGGAG 5 cut(s) 6, 434, 479, 623, 626
BseXI GCAGC 1 cut(s) 500
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 1 cut(s) 358
BshFI GGCC 1 cut(s) 43
BsiHKAI GWGCWC 3 cut(s) 206, 384, 500
BsiSI CCGG 2 cut(s) 218, 377
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 359
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 1 cut(s) 43
Bsp119I TTCGAA 1 cut(s) 409
Bsp1286I GDGCHC 3 cut(s) 206, 384, 500
Bsp143I GATC 6 cut(s) 49, 55, 103, 256, 270, 313
BspACI CCGC 1 cut(s) 311
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 358
BspMAI CTGCAG 1 cut(s) 115
BspPI GGATC 2 cut(s) 50, 321
BspT104I TTCGAA 1 cut(s) 409
BsrDI GCAATG 3 cut(s) 290, 299, 455
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 6 cut(s) 49, 55, 103, 256, 270, 313
BssNAI GTATAC 1 cut(s) 134
Bst1107I GTATAC 1 cut(s) 134
Bst4CI ACNGT 3 cut(s) 156, 321, 352
BstBI TTCGAA 1 cut(s) 409
BstDEI CTNAG 3 cut(s) 159, 225, 441
BstF5I GGATG 3 cut(s) 44, 119, 179
BstFNI CGCG 1 cut(s) 358
BstKTI GATC 6 cut(s) 52, 58, 106, 259, 273, 316
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 6 cut(s) 49, 55, 103, 256, 270, 313
BstMWI GCNNNNNNNGC 3 cut(s) 301, 388, 515
BstNSI RCATGY 1 cut(s) 522
BstSCI CCNGG 2 cut(s) 216, 375
BstSFI CTRYAG 1 cut(s) 111
BstUI CGCG 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 500
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BstZ17I GTATAC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 43
BtsCI GGATG 3 cut(s) 44, 119, 179
Cfr13I GGNCC 2 cut(s) 42, 82
CviAII CATG 3 cut(s) 461, 519, 736
DdeI CTNAG 3 cut(s) 159, 225, 441
DpnI GATC 6 cut(s) 51, 57, 105, 258, 272, 315
DpnII GATC 6 cut(s) 49, 55, 103, 256, 270, 313
Ecl136II GAGCTC 2 cut(s) 382, 498
Eco24I GRGCYC 2 cut(s) 384, 500
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 82
Eco53kI GAGCTC 2 cut(s) 382, 498
EcoICRI GAGCTC 2 cut(s) 382, 498
EcoRV GATATC 1 cut(s) 592
EcoT38I GRGCYC 2 cut(s) 384, 500
FaeI CATG 3 cut(s) 464, 522, 739
FaqI GGGAC 1 cut(s) 359
FatI CATG 3 cut(s) 460, 518, 735
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 1 cut(s) 489
FokI GGATG 3 cut(s) 51, 106, 166
FriOI GRGCYC 2 cut(s) 384, 500
Fsp4HI GCNGC 1 cut(s) 489
FspBI CTAG 2 cut(s) 246, 707
GluI GCNGC 1 cut(s) 489
GsuI CTGGAG 2 cut(s) 190, 675
HaeIII GGCC 1 cut(s) 43
HapII CCGG 2 cut(s) 218, 377
Hin1II CATG 3 cut(s) 464, 522, 739
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 2 cut(s) 116, 647
HpaII CCGG 2 cut(s) 218, 377
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 4 cut(s) 160, 345, 495, 589
Hpy188III TCNNGA 3 cut(s) 53, 432, 654
Hpy8I GTNNAC 1 cut(s) 134
Hpy99I CGWCG 1 cut(s) 264
HpyAV CCTTC 3 cut(s) 137, 652, 751
HpyCH4III ACNGT 3 cut(s) 156, 321, 352
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 4 cut(s) 113, 455, 460, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 301, 388, 515
HpyF3I CTNAG 3 cut(s) 159, 225, 441
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 3 cut(s) 464, 522, 739
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 6 cut(s) 49, 55, 103, 256, 270, 313
LmnI GCTCC 6 cut(s) 209, 379, 387, 421, 495, 503
LpnPI CCDG 7 cut(s) 129, 220, 231, 321, 390, 639, 671
Lsp1109I GCAGC 1 cut(s) 500
MaeI CTAG 2 cut(s) 246, 707
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 321, 725
MalI GATC 6 cut(s) 51, 57, 105, 258, 272, 315
MboI GATC 6 cut(s) 49, 55, 103, 256, 270, 313
MboII GAAGA 4 cut(s) 280, 481, 577, 630
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 3 cut(s) 206, 384, 500
MluCI AATT 7 cut(s) 405, 546, 552, 577, 601, 673, 680
MlyI GAGTC 2 cut(s) 125, 656
MseI TTAA 5 cut(s) 234, 404, 528, 722, 760
MspI CCGG 2 cut(s) 218, 377
MspR9I CCNGG 2 cut(s) 218, 377
Mva1269I GAATGC 1 cut(s) 339
MvnI CGCG 1 cut(s) 358
MwoI GCNNNNNNNGC 3 cut(s) 301, 388, 515
NciI CCSGG 2 cut(s) 218, 377
NdeII GATC 6 cut(s) 49, 55, 103, 256, 270, 313
NlaIII CATG 3 cut(s) 464, 522, 739
NmuCI GTSAC 1 cut(s) 725
NspI RCATGY 1 cut(s) 522
NspV TTCGAA 1 cut(s) 409
PctI GAATGC 1 cut(s) 339
PflMI CCANNNNNTGG 1 cut(s) 39
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 1 cut(s) 490
PleI GAGTC 2 cut(s) 124, 655
PpsI GAGTC 2 cut(s) 124, 655
PshBI ATTAAT 1 cut(s) 404
Psp124BI GAGCTC 2 cut(s) 384, 500
PspPI GGNCC 2 cut(s) 42, 82
PstI CTGCAG 1 cut(s) 115
PsuI RGATCY 1 cut(s) 55
SacI GAGCTC 2 cut(s) 384, 500
SaqAI TTAA 5 cut(s) 234, 404, 528, 722, 760
SatI GCNGC 1 cut(s) 489
Sau3AI GATC 6 cut(s) 49, 55, 103, 256, 270, 313
Sau96I GGNCC 2 cut(s) 42, 82
SchI GAGTC 2 cut(s) 125, 656
ScrFI CCNGG 2 cut(s) 218, 377
SduI GDGCHC 3 cut(s) 206, 384, 500
SfcI CTRYAG 1 cut(s) 111
SfuI TTCGAA 1 cut(s) 409
SinI GGWCC 1 cut(s) 82
Sse9I AATT 7 cut(s) 405, 546, 552, 577, 601, 673, 680
SsiI CCGC 1 cut(s) 311
SspI AATATT 1 cut(s) 758
SspMI CTAG 2 cut(s) 246, 707
SstI GAGCTC 2 cut(s) 384, 500
StyD4I CCNGG 2 cut(s) 216, 375
TaaI ACNGT 3 cut(s) 156, 321, 352
TaiI ACGT 1 cut(s) 375
TaqI TCGA 2 cut(s) 409, 645
TasI AATT 7 cut(s) 405, 546, 552, 577, 601, 673, 680
Tru1I TTAA 5 cut(s) 234, 404, 528, 722, 760
Tru9I TTAA 5 cut(s) 234, 404, 528, 722, 760
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 1 cut(s) 488
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 1 cut(s) 288
Van91I CCANNNNNTGG 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 82
VspI ATTAAT 1 cut(s) 404
XapI RAATTY 2 cut(s) 552, 601
XceI RCATGY 1 cut(s) 522
XmiI GTMKAC 1 cut(s) 133
XspI CTAG 2 cut(s) 246, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.