MD14G1086600.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003398
Physical Location & Seq
Forward (+)
10720558 .. 10722298
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1086600.v1.1.491

Sequence Viewer

Length: 789 bp
ATGTTAGCCTCGTCTCCTCCTTTGTTTTCAACCATTGGATGGCCCTTAGATCAAGATTCTATTAGCCATGAGTACCACAACTACTTCTACAGCAACGACATTACTACTACTAATGATGATCAAACTGCAGACTCATTCCTTCATTTACTTCCATGTCATCACCCACAAGTTGACCTTGATCGTTACACACCATCCACAACTGTCACCGGTGACTACTCCAGCGTTTGTCCGATGGCAAAGAAACTCAACCACAATGCTAGTGAGCGTGATCGTCGCAAGAAAATCAGTAACTTGTATGCCTCATTGCGTTCACTCCTTCCTGCAGATCAAACGAAAAAACTAAGCATTCCGAATACAATTTCGCGTGCACTGAAATACATACCGGAACTCCAAAAGCAAGTGAAGGGACTGAACCGAAAGAGGGAAGAACTCTTATCAAGAGCTTCTAAGCAAGAAGATGCAATGCATGAGGAGAAAAAAATAAAAACCACAGCTCGGAGTTCACAATCTGCAGTTTCAACCTACCGTCTTAACGATAGAGAAGTAGCAATTCAAATATCCACCTTTAAGACCCACAACAATCTATTATCTGAGATCTTACAACATCTGGAGGAAGAGGGGCTTGAACTAGAAAATGCTTCTTTCTTTGAGTCCTATGGAGGCAGGGTTTTCTATAATTTACATCTTCAGGTGGACAGAACATACAGATTAGAGTGTGAGAAAGAAGCTTATGTCCTTCTATGCATGACAAAAGGAAAGAGCCATTCCCATAAGAATTTTGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0006357 GO:0006873 GO:0006875 GO:0006879 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010104 GO:0010106 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0019219 GO:0019222 GO:0019725 GO:0023051 GO:0030003 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034248 GO:0034250 GO:0042221 GO:0042493 GO:0042592 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0043565 GO:0044212 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0046685 GO:0046686 GO:0046916 GO:0048518 GO:0048522 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051171 GO:0051173 GO:0051193 GO:0051194 GO:0051252 GO:0051254 GO:0051716 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0060255 GO:0065007 GO:0065008 GO:0070297 GO:0071496 GO:0071731 GO:0080090 GO:0090575 GO:0097159 GO:0097366 GO:0098771 GO:0140110 GO:1900376 GO:1900378 GO:1900704 GO:1900706 GO:1901363 GO:1901698 GO:1901700 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990641 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

263

Amino Acids

30.17

Weight (kDa)

6.87

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 80 - 131 5.3e-13 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 39
AccII CGCG 1 cut(s) 364
AcsI RAATTY 1 cut(s) 775
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 3 cut(s) 39, 421, 495
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 5 cut(s) 30, 519, 554, 626, 782
AluBI AGCT 3 cut(s) 443, 494, 728
AluI AGCT 3 cut(s) 443, 494, 728
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 1 cut(s) 18
Alw44I GTGCAC 1 cut(s) 366
AoxI GGCC 1 cut(s) 41
ApaLI GTGCAC 1 cut(s) 366
ApoI RAATTY 1 cut(s) 775
AsiGI ACCGGT 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 42
AsuHPI GGTGA 3 cut(s) 152, 196, 221
BaeGI GKGCMC 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 370
BccI CCATC 3 cut(s) 33, 199, 226
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 3 cut(s) 258, 629, 787
BfmI CTRYAG 4 cut(s) 88, 126, 321, 510
BglII AGATCT 1 cut(s) 594
BmgT120I GGNCC 1 cut(s) 42
BmsI GCATC 1 cut(s) 448
BpmI CTGGAG 2 cut(s) 202, 629
BsaWI WCCGGW 2 cut(s) 206, 382
BsaXI ACNNNNNCTCC 4 cut(s) 372, 402, 651, 681
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 421, 495
Bse118I RCCGGY 1 cut(s) 206
Bse3DI GCAATG 2 cut(s) 302, 468
BseGI GGATG 2 cut(s) 44, 191
BseLI CCNNNNNNNGG 3 cut(s) 39, 421, 495
BseMI GCAATG 2 cut(s) 302, 468
BseMII CTCAG 1 cut(s) 582
BseRI GAGGAG 2 cut(s) 6, 485
BseSI GKGCMC 1 cut(s) 370
Bsh1236I CGCG 1 cut(s) 364
BshFI GGCC 1 cut(s) 43
BshTI ACCGGT 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 2 cut(s) 207, 383
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 3 cut(s) 39, 421, 495
BsmAI GTCTC 1 cut(s) 18
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 420
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 370
Bsp143I GATC 6 cut(s) 49, 118, 178, 268, 325, 594
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 583
BspFNI CGCG 1 cut(s) 364
BspMAI CTGCAG 3 cut(s) 130, 325, 514
BsrDI GCAATG 2 cut(s) 302, 468
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssMI GATC 6 cut(s) 49, 118, 178, 268, 325, 594
Bst4CI ACNGT 2 cut(s) 202, 527
Bst6I CTCTTC 1 cut(s) 609
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 4 cut(s) 46, 341, 447, 591
BstF5I GGATG 2 cut(s) 44, 191
BstFNI CGCG 1 cut(s) 364
BstKTI GATC 6 cut(s) 52, 121, 181, 271, 328, 597
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 6 cut(s) 49, 118, 178, 268, 325, 594
BstSFI CTRYAG 4 cut(s) 88, 126, 321, 510
BstSLI GKGCMC 1 cut(s) 370
BstUI CGCG 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 594
BstYI RGATCY 1 cut(s) 594
BsuRI GGCC 1 cut(s) 43
BtsCI GGATG 2 cut(s) 44, 191
BtsIMutI CAGTG 1 cut(s) 368
Cac8I GCNNGC 1 cut(s) 366
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 73
CspAI ACCGGT 1 cut(s) 206
CviAII CATG 4 cut(s) 68, 153, 467, 745
CviJI RGCY 8 cut(s) 8, 43, 66, 443, 494, 622, 728, 762
CviKI_1 RGCY 8 cut(s) 8, 43, 66, 443, 494, 622, 728, 762
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 4 cut(s) 46, 341, 447, 591
DpnI GATC 6 cut(s) 51, 120, 180, 270, 327, 596
DpnII GATC 6 cut(s) 49, 118, 178, 268, 325, 594
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
Eco57I CTGAAG 1 cut(s) 671
EcoT22I ATGCAT 2 cut(s) 468, 746
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 4 cut(s) 71, 156, 470, 748
FalI AAGNNNNNCTT 4 cut(s) 159, 191, 606, 638
FaqI GGGAC 1 cut(s) 420
FatI CATG 4 cut(s) 67, 152, 466, 744
FbaI TGATCA 1 cut(s) 118
FokI GGATG 2 cut(s) 51, 178
FspBI CTAG 3 cut(s) 258, 629, 787
GsuI CTGGAG 2 cut(s) 202, 629
HaeIII GGCC 1 cut(s) 43
HapII CCGG 2 cut(s) 207, 383
Hin1II CATG 4 cut(s) 71, 156, 470, 748
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 3 cut(s) 56, 131, 650
HpaII CCGG 2 cut(s) 207, 383
HphI GGTGA 3 cut(s) 152, 196, 221
Hpy166II GTNNAC 5 cut(s) 172, 311, 368, 503, 694
Hpy188I TCNGA 4 cut(s) 231, 351, 498, 592
Hpy188III TCNNGA 3 cut(s) 53, 438, 608
Hpy8I GTNNAC 5 cut(s) 172, 311, 368, 503, 694
Hpy99I CGWCG 1 cut(s) 276
HpyAV CCTTC 4 cut(s) 149, 326, 397, 746
HpyCH4III ACNGT 2 cut(s) 202, 527
HpyCH4V TGCA 7 cut(s) 128, 323, 368, 461, 466, 512, 744
HpyF3I CTNAG 4 cut(s) 46, 341, 447, 591
Hsp92II CATG 4 cut(s) 71, 156, 470, 748
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 6 cut(s) 49, 118, 178, 268, 325, 594
LpnPI CCDG 7 cut(s) 220, 232, 333, 396, 593, 649, 674
LweI GCATC 1 cut(s) 448
MaeI CTAG 3 cut(s) 258, 629, 787
MaeIII GTNAC 4 cut(s) 182, 202, 209, 287
MalI GATC 6 cut(s) 51, 120, 180, 270, 327, 596
MboI GATC 6 cut(s) 49, 118, 178, 268, 325, 594
MboII GAAGA 4 cut(s) 437, 467, 626, 677
MflI RGATCY 1 cut(s) 594
MhlI GDGCHC 1 cut(s) 370
MluCI AATT 4 cut(s) 357, 549, 676, 775
MlyI GAGTC 2 cut(s) 125, 659
MnlI CCTC 8 cut(s) 19, 27, 310, 414, 463, 604, 610, 653
Mph1103I ATGCAT 2 cut(s) 468, 746
MseI TTAA 2 cut(s) 531, 567
MspI CCGG 2 cut(s) 207, 383
Mva1269I GAATGC 1 cut(s) 345
MvnI CGCG 1 cut(s) 364
NdeII GATC 6 cut(s) 49, 118, 178, 268, 325, 594
NlaIII CATG 4 cut(s) 71, 156, 470, 748
NmuCI GTSAC 2 cut(s) 202, 209
NsiI ATGCAT 2 cut(s) 468, 746
PctI GAATGC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 56
PflMI CCANNNNNTGG 1 cut(s) 39
PinAI ACCGGT 1 cut(s) 206
PleI GAGTC 2 cut(s) 125, 658
PpsI GAGTC 2 cut(s) 125, 658
PspPI GGNCC 1 cut(s) 42
PstI CTGCAG 3 cut(s) 130, 325, 514
PsuI RGATCY 1 cut(s) 594
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
SaqAI TTAA 2 cut(s) 531, 567
Sau3AI GATC 6 cut(s) 49, 118, 178, 268, 325, 594
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 2 cut(s) 125, 659
SduI GDGCHC 1 cut(s) 370
SetI ASST 7 cut(s) 177, 445, 496, 524, 566, 693, 730
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 4 cut(s) 88, 126, 321, 510
SgrAI CRCCGGYG 1 cut(s) 206
Sse9I AATT 4 cut(s) 357, 549, 676, 775
SspMI CTAG 3 cut(s) 258, 629, 787
TaaI ACNGT 2 cut(s) 202, 527
TasI AATT 4 cut(s) 357, 549, 676, 775
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 2 cut(s) 531, 567
Tru9I TTAA 2 cut(s) 531, 567
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 2 cut(s) 202, 209
Tsp45I GTSAC 2 cut(s) 202, 209
TspDTI ATGAA 1 cut(s) 131
TspRI CASTG 1 cut(s) 375
Van91I CCANNNNNTGG 1 cut(s) 39
VneI GTGCAC 1 cut(s) 366
XapI RAATTY 1 cut(s) 775
XspI CTAG 3 cut(s) 258, 629, 787
Zsp2I ATGCAT 2 cut(s) 468, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.