MD14G1142000.v1.1

Nuclear pore complex protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007827
Physical Location & Seq
Forward (+)
23465594 .. 23472220
6627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1142000.v1.1.491

Sequence Viewer

Length: 3069 bp
ATGTTCGGTTCTTCTAATCCCTTTGGACAGTCATCTAGCAGCCCGTTTGGGTCCCAATCGGTATTTGGGCAGACCAGCAGCACAAATAACAATCCTTTTGCCCCTAAGCCTTTTGGTAGTACAACCCCATTTGGTGCGCAAACAGGGAGTACCATGTTTGGAGGAACTTCAACTGGTGTATTTGGCGCAGCTCAAGCTTCTTCACCTTTTCCTTCCTCCACAACCTTTGGTGCTGCGTCCTCACCGGCTTTTGGAAATACAACGCCTGCTTTTGGAGCAACTTCTAGTCCTTCACCATCACCTTTTGGTGGTTCATCTGGTTTTGGTCAGAAGCCTGTGTTTGGAGCATTTGGGTCCAATCCTGCTCAAACAAGTCCTTTTGGAAGTACAACTCAACCATCACAGCCAGCATTTGGGAGCAGTGTATTTGGTTCCTCCACGCCATTTGGTGGATCCAGTCAGCCAGCATTTGGTGCTACCACCACCCCAGCTTTTGGTGCTACAAGCACCCCAGCTTTTGGTGCTACAAGCACCCCAGCCTTTGGTGCTACGAGTAGTCCAGCCTTTGGTGCTACAAGTAGTCCAGCCTTTGGTGCTACCACCACCCCAGCTTTTGGTGCTACCACCACCCCCGCTTTTGGTGCTACCAGCACGCCAGCTTTTGGTGCTGCCACCACCCCAGCATTTGGAGCTACAAGTAGCCCGGCCTTTGGTTCCACAACAAGTCCTACATTTGGAAGCACAGGAGCTGCATTTGGGGTGTCGAGTTCTCCCTTGTTTGGATCTGGGGGAGCTTTTGGGGCTTCAAGCACCCCGGCCTTTGGTTCATCTAGCTCGACCTTTGGTACTTCAACCAATCCTGCTTTTGGTGCTTCAAGTGCTCCTGGTTTTGGTTCGTCCAGTACTCCATCGTTCAGCTTTCCATCAACTCCTACCTTTGGCCAATCAAGTTCTGCATTTGGTAGCAGCCAGTTTGCCGCTTCATCGCCTTTTGGAGCACAGAGTTCTCCGTTTGGAGCTCAATCAACAACATTTGGGAACACTGGTGCCTTTGGGCAGTCAGCTTTTGGGGGCCAACAACGCGGGGGCAGTAGAGTGGCTGCATATGCTGCAACTCCTGAACCCGATGGTGGCACTGGGGCTAGTGCTGCTGGGAAATTGGAGTCAATATCAGCAATGCCTGCATATAAAGAAAAAAGTCATGAAGAACTCCGCTGGGAGGATTATCAATTGGGGGATAAAGGTGGTCCAGCTCCTGCTGGTTCGAGTGGCTTTGGCATTACCACCTCACAGCCAAACCCTTTGAATCCTGCACAGGCATTTCAAGCCTCAAGTCCTTTTAATGCCACAACTTCATCTAATTTGTTTGCTCCAAAAACTCCATCCTTCACTAATACAGGCTTTGCAACATCGTCTACACCATTCAGTTCATCAGGTTTTGGGATTTCTACTTCAACTAGTCCTTTTACACCATCACAACCTTCTACAGTTTTCCCGCAAACCTCATCTTCGTCTCTCTTTAGTTCGTCAAGCTCTGCATATTCATTTCCTGCTCCGGCTGCGACAGCTACTTCCACATTTAATTCAAACCCGTTCAATCCCACACCTCCAGCTGCCCAGACAGGTGGTGCTTTTACCAATCCATTTGGGCAGAATACAACATTTTCTCAGCCATTCAGTAACCCTTCTACTGGCTTTGGTTTAACAATCTCCACTGCTGCTGGTGCTACAAGCAGTCAAATGGGTTTTAGTCAACCAGCTCCAACTTTTTCTGTGCCTTTCTCATCTCAGCCAACTCAGAATAGTGGTTTTAACTTCAACAGTTTTAATCAAACCCAATCAGGCAACACAGGTGGTTTGGGAAGTTCAACAGGAATTCTTGGTCAGAATAACTTTGGACAGTTGCCTGCCACTCAAAGCTCTGTAGCTGTACAACCAGTACCTGCTACAAATCCATTTGGAACGCTCCCTGCAATGCCACAGATGTCAATTGGTCGTGGTGGAACTGCACCGTCAATTCAATATGGAATTTCAAGCATGCCTGTCGTAGACAAACCTGCGCCTGTTAAGGTATCATATTTGTTGACCTCTCGACACCTGTCACAAAGGCGGGTCAGGTTACCTGCGAGAAAATATAATCCTAAGAATGATGATCCAAGGTTCCCATTCTTTGTTGACGATGAAGAAACAGATAACACACCCAAGGCGGATGCTCTTTTCCTTCCCAGAGAAAACCCAAGAGCACTCGTCATTCGTCCAAAAGAACAGTGGCCTCAAAGGGTTGGTGCTGAGAAAGCATCCCCATTAAAGGATACTTCGACTCCAGTGCATGAGAATGGAAAAATTCTAGGAGAAGCAGGCTCTGAGGATAAAGATAAAACGCCAAAAGAAAATGGAATTGTGAAAGAGCGGGCGAATCATGTCAAAGTCAACCAGAAACCTAATGGAATTCATGATGATCATTCTGTTCAGAAAGGGGACTCATATATGACACTAAGTGGGCATAGAGCCGGTGAGGCTGCTATTGCATATGAGCATGGTGCTGACATTGAGGCCCTAATGCCAAAGCTCCGGCGTACAGACTATTATACAGAGCCTCGAATCCAAGAATTGGCAGCTAAGGAAAGGTTGGACCCGGGGTTCTGCTGTCGTGTCAAGGACTTTGTGGTTGGACGAGTTGGGTATGGTAGCATCAAGTTCTTTGGGGAGACAGATGTGCGATGCCTTGATCTCGAGGCTCTGGTGCAGTTCAACAATCGGGAGGTGATAGTGTACATGGATGACAATAAGAAACCTCCCGTCGGGCAAGGCCTCAACAAACCTGCTGAGGTTACACTTCTCAACATTACATGCACTGACAAGAAAACAGGACGTCAGTTCACAGAAGGCCCAAAGACTGAGAGATACAAGCAGATGCTGAAGAAGAAGGCAGAGGATCAAGGAGCCGAATTTGTTTCTTATGATGCCTTGAAAGGCGAGTGGAAGTTCAGGGTAAAGCACTTCAGTAAGTACGAGTTGGGAGAGGAAGACGACTGGGATACCGATGCTGCTGCTGCCCAAGACTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000972 GO:0000973 GO:0003674 GO:0003676 GO:0003682 GO:0003712 GO:0003713 GO:0003723 GO:0003729 GO:0005048 GO:0005198 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006999 GO:0008104 GO:0008139 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016032 GO:0016043 GO:0016234 GO:0016604 GO:0017038 GO:0017056 GO:0019219 GO:0019222 GO:0022607 GO:0031080 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0033036 GO:0033120 GO:0033218 GO:0033365 GO:0034397 GO:0034398 GO:0034399 GO:0034504 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0042277 GO:0042405 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044614 GO:0044615 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0046931 GO:0048024 GO:0048026 GO:0048518 GO:0048522 GO:0048583 GO:0050000 GO:0050657 GO:0050658 GO:0050684 GO:0050685 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051276 GO:0051292 GO:0051640 GO:0051641 GO:0051649 GO:0051704 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090304 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1902446 GO:1902680 GO:1903311 GO:1903313 GO:1903506 GO:1903508 GO:1990841 GO:1990904 GO:2000030 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
Nucleoporin2

Protein Analysis

1023

Amino Acids

105.19

Weight (kDa)

8.06

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nup98_GLEBS PF21240 389 - 409 2.6e-06 Nup98, Gle2-binding sequence
Nucleoporin2 PF04096 860 - 1006 2.8e-44 Nup98-96 autopeptidase S59
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2875
Acc16I TGCGCA 1 cut(s) 138
Acc36I ACCTGC 4 cut(s) 1951, 2065, 2131, 2830
AccB1I GGYRCC 1 cut(s) 1046
AccBSI CCGCTC 1 cut(s) 2410
AccI GTMKAC 2 cut(s) 1415, 2049
AccII CGCG 1 cut(s) 1083
AciI CCGC 8 cut(s) 633, 978, 1083, 1213, 1496, 2110, 2207, 2410
AclWI GGATC 5 cut(s) 447, 460, 790, 2147, 2943
AcoI YGGCCR 1 cut(s) 940
AcsI RAATTY 5 cut(s) 1875, 2028, 2343, 2448, 2948
AcuI CTGAAG 2 cut(s) 2939, 2986
AcyI GRCGYC 1 cut(s) 2872
AdeI CACNNNGTG 1 cut(s) 2498
AhlI ACTAGT 1 cut(s) 1457
AjnI CCWGG 1 cut(s) 883
AloI GAACNNNNNNTCC 2 cut(s) 2624, 2656
Alw21I GWGCWC 4 cut(s) 883, 1000, 1021, 2245
Alw26I GTCTC 2 cut(s) 1518, 2702
AlwI GGATC 5 cut(s) 447, 460, 790, 2147, 2943
AlwNI CAGNNNCTG 5 cut(s) 749, 1256, 1943, 2363, 2917
Ama87I CYCGRG 2 cut(s) 2635, 2732
AoxI GGCC 8 cut(s) 705, 816, 940, 1072, 2270, 2553, 2809, 2887
ApoI RAATTY 5 cut(s) 1875, 2028, 2343, 2448, 2948
AspLEI GCGC 3 cut(s) 139, 188, 2062
AspS9I GGNCC 7 cut(s) 51, 354, 1072, 1247, 2554, 2632, 2888
AsuC2I CCSGG 4 cut(s) 704, 815, 2636, 2637
AsuHPI GGTGA 6 cut(s) 195, 234, 285, 291, 2525, 2776
AvaI CYCGRG 2 cut(s) 2635, 2732
AvaII GGWCC 4 cut(s) 51, 354, 1247, 2632
BalI TGGCCA 1 cut(s) 942
BamHI GGATCC 1 cut(s) 452
BanI GGYRCC 1 cut(s) 1046
BanII GRGCYC 1 cut(s) 1021
BbsI GAAGAC 1 cut(s) 3033
Bbv12I GWGCWC 4 cut(s) 883, 1000, 1021, 2245
BbvCI CCTCAGC 1 cut(s) 2826
BccI CCATC 7 cut(s) 304, 406, 916, 931, 1121, 1390, 1480
BcgI CGANNNNNNTGC 6 cut(s) 2026, 2060, 2308, 2342, 3032, 3066
BciT130I CCWGG 1 cut(s) 885
BciVI GTATCC 2 cut(s) 2305, 3031
BclI TGATCA 1 cut(s) 2458
BcnI CCSGG 4 cut(s) 704, 815, 2636, 2637
BcoDI GTCTC 2 cut(s) 1518, 2702
BcuI ACTAGT 1 cut(s) 1457
BfaI CTAG 6 cut(s) 36, 285, 831, 1143, 1458, 2348
BfmI CTRYAG 2 cut(s) 1485, 1923
BfuAI ACCTGC 4 cut(s) 1951, 2065, 2131, 2830
BfuI GTATCC 2 cut(s) 2305, 3031
BglI GCCNNNNNGGC 1 cut(s) 2516
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 5 cut(s) 704, 815, 885, 2636, 2637
Bme18I GGWCC 4 cut(s) 51, 354, 1247, 2632
BmeT110I CYCGRG 2 cut(s) 2635, 2732
BmgT120I GGNCC 7 cut(s) 51, 354, 1072, 1247, 2554, 2632, 2888
BmrFI CCNGG 5 cut(s) 704, 815, 885, 2636, 2637
BmrI ACTGGG 2 cut(s) 1146, 3043
BmsI GCATC 7 cut(s) 2200, 2306, 2700, 2711, 2904, 2953, 3034
BmuI ACTGGG 2 cut(s) 1146, 3043
BoxI GACNNNNGTC 1 cut(s) 2098
BpiI GAAGAC 1 cut(s) 3033
BpmI CTGGAG 2 cut(s) 1593, 2307
Bpu10I CCTNAGC 3 cut(s) 105, 2619, 2826
BpuEI CTTGAG 2 cut(s) 177, 1315
BpuMI CCSGG 4 cut(s) 704, 815, 2636, 2637
BsaHI GRCGYC 1 cut(s) 2872
BsaJI CCNNGG 5 cut(s) 813, 2156, 2202, 2635, 2636
BsaXI ACNNNNNCTCC 2 cut(s) 2305, 2335
Bse118I RCCGGY 2 cut(s) 244, 2510
Bse3DI GCAATG 2 cut(s) 1182, 1980
BseBI CCWGG 1 cut(s) 885
BseDI CCNNGG 5 cut(s) 813, 2156, 2202, 2635, 2636
BseGI GGATG 4 cut(s) 1382, 2215, 2297, 2785
BseMI GCAATG 2 cut(s) 1182, 1980
BseMII CTCAG 7 cut(s) 1682, 1802, 1811, 2280, 2355, 2817, 2889
BseYI CCCAGC 7 cut(s) 487, 511, 535, 607, 679, 1151, 1215
BsgI GTGCAG 3 cut(s) 1296, 1992, 2765
Bsh1236I CGCG 1 cut(s) 1083
BshFI GGCC 8 cut(s) 707, 818, 942, 1074, 2272, 2555, 2811, 2889
BshNI GGYRCC 1 cut(s) 1046
BsiHKAI GWGCWC 4 cut(s) 883, 1000, 1021, 2245
BsiHKCI CYCGRG 2 cut(s) 2635, 2732
BsiSI CCGG 7 cut(s) 245, 704, 815, 1556, 2511, 2572, 2636
BslFI GGGAC 2 cut(s) 37, 2492
BsmAI GTCTC 2 cut(s) 1518, 2702
BsmBI CGTCTC 1 cut(s) 1518
BsmFI GGGAC 2 cut(s) 37, 2492
BsnI GGCC 8 cut(s) 707, 818, 942, 1074, 2272, 2555, 2811, 2889
BsoBI CYCGRG 2 cut(s) 2635, 2732
Bsp1286I GDGCHC 4 cut(s) 883, 1000, 1021, 2245
Bsp1407I TGTACA 2 cut(s) 1930, 2772
Bsp143I GATC 6 cut(s) 452, 782, 2152, 2458, 2728, 2935
BspACI CCGC 8 cut(s) 633, 978, 1083, 1213, 1496, 2110, 2207, 2410
BspANI GGCC 8 cut(s) 707, 818, 942, 1074, 2272, 2555, 2811, 2889
BspCNI CTCAG 7 cut(s) 1681, 1801, 1810, 2281, 2356, 2818, 2890
BspFNI CGCG 1 cut(s) 1083
BspHI TCATGA 2 cut(s) 1201, 2452
BspMI ACCTGC 4 cut(s) 1951, 2065, 2131, 2830
BspPI GGATC 5 cut(s) 447, 460, 790, 2147, 2943
BspT107I GGYRCC 1 cut(s) 1046
BsrBI CCGCTC 1 cut(s) 2410
BsrDI GCAATG 2 cut(s) 1182, 1980
BsrFI RCCGGY 2 cut(s) 244, 2510
BsrGI TGTACA 2 cut(s) 1930, 2772
BssAI RCCGGY 2 cut(s) 244, 2510
BssECI CCNNGG 5 cut(s) 813, 2156, 2202, 2635, 2636
BssMI GATC 6 cut(s) 452, 782, 2152, 2458, 2728, 2935
BssNI GRCGYC 1 cut(s) 2872
BssT1I CCWWGG 2 cut(s) 2156, 2202
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 6 cut(s) 30, 1489, 1823, 1902, 2013, 2268
BstACI GRCGYC 1 cut(s) 2872
BstAPI GCANNNNNTGC 3 cut(s) 473, 1109, 1181
BstAUI TGTACA 2 cut(s) 1930, 2772
BstEII GGTNACC 1 cut(s) 2118
BstF5I GGATG 4 cut(s) 1382, 2215, 2297, 2785
BstFNI CGCG 1 cut(s) 1083
BstHHI GCGC 3 cut(s) 139, 188, 2062
BstKTI GATC 6 cut(s) 455, 785, 2155, 2461, 2731, 2938
BstMAI GTCTC 2 cut(s) 1518, 2702
BstMBI GATC 6 cut(s) 452, 782, 2152, 2458, 2728, 2935
BstNI CCWGG 1 cut(s) 885
BstNSI RCATGY 2 cut(s) 2041, 2853
BstPAI GACNNNNGTC 1 cut(s) 2098
BstPI GGTNACC 1 cut(s) 2118
BstSCI CCNGG 5 cut(s) 702, 813, 883, 2634, 2635
BstSFI CTRYAG 2 cut(s) 1485, 1923
BstUI CGCG 1 cut(s) 1083
BstV2I GAAGAC 1 cut(s) 3033
BstX2I RGATCY 2 cut(s) 452, 782
BstXI CCANNNNNNTGG 1 cut(s) 1625
BstYI RGATCY 2 cut(s) 452, 782
BsuI GTATCC 2 cut(s) 2305, 3031
BsuRI GGCC 8 cut(s) 707, 818, 942, 1074, 2272, 2555, 2811, 2889
BtgZI GCGATG 2 cut(s) 969, 2734
BtsCI GGATG 4 cut(s) 1382, 2215, 2297, 2785
BtsI GCAGTG 2 cut(s) 427, 1713
BtsIMutI CAGTG 7 cut(s) 427, 1041, 1134, 1713, 2273, 2331, 2853
BveI ACCTGC 4 cut(s) 1951, 2065, 2131, 2830
CaiI CAGNNNCTG 5 cut(s) 749, 1256, 1943, 2363, 2917
CciI TCATGA 2 cut(s) 1201, 2452
CfoI GCGC 3 cut(s) 139, 188, 2062
Cfr10I RCCGGY 2 cut(s) 244, 2510
Cfr13I GGNCC 7 cut(s) 51, 354, 1072, 1247, 2554, 2632, 2888
Cfr9I CCCGGG 1 cut(s) 2635
CseI GACGC 1 cut(s) 225
CspCI CAANNNNNGTGG 6 cut(s) 208, 243, 427, 462, 1834, 1869
CviAII CATG 9 cut(s) 154, 1202, 2038, 2330, 2420, 2453, 2537, 2776, 2850
DpnI GATC 6 cut(s) 454, 784, 2154, 2460, 2730, 2937
DpnII GATC 6 cut(s) 452, 782, 2152, 2458, 2728, 2935
DraIII CACNNNGTG 1 cut(s) 2498
EaeI YGGCCR 1 cut(s) 940
EciI GGCGGA 1 cut(s) 2222
Ecl136II GAGCTC 1 cut(s) 1019
Eco130I CCWWGG 2 cut(s) 2156, 2202
Eco147I AGGCCT 1 cut(s) 2811
Eco24I GRGCYC 1 cut(s) 1021
Eco47I GGWCC 4 cut(s) 51, 354, 1247, 2632
Eco53kI GAGCTC 1 cut(s) 1019
Eco57I CTGAAG 2 cut(s) 2939, 2986
Eco88I CYCGRG 2 cut(s) 2635, 2732
Eco91I GGTNACC 1 cut(s) 2118
EcoICRI GAGCTC 1 cut(s) 1019
EcoO109I RGGNCCY 2 cut(s) 51, 2554
EcoO65I GGTNACC 1 cut(s) 2118
EcoRI GAATTC 2 cut(s) 1875, 2448
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 2 cut(s) 2156, 2202
EcoT38I GRGCYC 1 cut(s) 1021
ErhI CCWWGG 2 cut(s) 2156, 2202
Esp3I CGTCTC 1 cut(s) 1518
FaeI CATG 9 cut(s) 157, 1205, 2041, 2333, 2423, 2456, 2540, 2779, 2853
FaqI GGGAC 2 cut(s) 37, 2492
FatI CATG 9 cut(s) 153, 1201, 2037, 2329, 2419, 2452, 2536, 2775, 2849
FauI CCCGC 5 cut(s) 640, 1076, 1503, 2103, 2403
FauNDI CATATG 2 cut(s) 1105, 2530
FbaI TGATCA 1 cut(s) 2458
FblI GTMKAC 2 cut(s) 1415, 2049
FokI GGATG 4 cut(s) 1369, 2222, 2284, 2792
FriOI GRGCYC 1 cut(s) 1021
FspBI CTAG 6 cut(s) 36, 285, 831, 1143, 1458, 2348
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 3 cut(s) 138, 187, 2061
GsaI CCCAGC 7 cut(s) 491, 515, 539, 611, 683, 1155, 1219
GsuI CTGGAG 2 cut(s) 1593, 2307
HaeIII GGCC 8 cut(s) 707, 818, 942, 1074, 2272, 2555, 2811, 2889
HapII CCGG 7 cut(s) 245, 704, 815, 1556, 2511, 2572, 2636
HgaI GACGC 1 cut(s) 225
HhaI GCGC 3 cut(s) 139, 188, 2062
Hin1I GRCGYC 1 cut(s) 2872
Hin1II CATG 9 cut(s) 157, 1205, 2041, 2333, 2423, 2456, 2540, 2779, 2853
Hin6I GCGC 3 cut(s) 137, 186, 2060
HinP1I GCGC 3 cut(s) 137, 186, 2060
HincII GTYRAC 4 cut(s) 1754, 2085, 2176, 2431
HindII GTYRAC 4 cut(s) 1754, 2085, 2176, 2431
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 6 cut(s) 1163, 1306, 2320, 2416, 2480, 2601
HpaII CCGG 7 cut(s) 245, 704, 815, 1556, 2511, 2572, 2636
HphI GGTGA 6 cut(s) 195, 234, 285, 291, 2525, 2776
Hpy166II GTNNAC 8 cut(s) 1416, 1754, 2050, 2085, 2176, 2431, 2773, 2880
Hpy188I TCNGA 5 cut(s) 330, 1800, 1887, 2365, 2472
Hpy188III TCNNGA 6 cut(s) 1118, 1202, 2091, 2453, 2732, 2759
Hpy8I GTNNAC 8 cut(s) 1416, 1754, 2050, 2085, 2176, 2431, 2773, 2880
Hpy99I CGWCG 1 cut(s) 2804
HpyAV CCTTC 8 cut(s) 222, 300, 1396, 1491, 1695, 2231, 2879, 2920
HpyCH4III ACNGT 6 cut(s) 30, 1489, 1823, 1902, 2013, 2268
HpyCH4IV ACGT 1 cut(s) 2872
HpySE526I ACGT 1 cut(s) 2872
Hsp92I GRCGYC 1 cut(s) 2872
Hsp92II CATG 9 cut(s) 157, 1205, 2041, 2333, 2423, 2456, 2540, 2779, 2853
HspAI GCGC 3 cut(s) 137, 186, 2060
KflI GGGWCCC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 2458
Kzo9I GATC 6 cut(s) 452, 782, 2152, 2458, 2728, 2935
LweI GCATC 7 cut(s) 2200, 2306, 2700, 2711, 2904, 2953, 3034
MaeI CTAG 6 cut(s) 36, 285, 831, 1143, 1458, 2348
MaeII ACGT 1 cut(s) 2872
MaeIII GTNAC 4 cut(s) 1679, 2100, 2118, 2830
MalI GATC 6 cut(s) 454, 784, 2154, 2460, 2730, 2937
MbiI CCGCTC 1 cut(s) 2410
MboI GATC 6 cut(s) 452, 782, 2152, 2458, 2728, 2935
MboII GAAGA 8 cut(s) 3, 192, 1217, 1500, 2195, 2932, 2935, 3038
MfeI CAATTG 2 cut(s) 1229, 1989
MflI RGATCY 2 cut(s) 452, 782
MhlI GDGCHC 4 cut(s) 883, 1000, 1021, 2245
MlsI TGGCCA 1 cut(s) 942
MluNI TGGCCA 1 cut(s) 942
MlyI GAGTC 3 cut(s) 1172, 2314, 2474
MmeI TCCRAC 3 cut(s) 1787, 2610, 2650
Mox20I TGGCCA 1 cut(s) 942
MscI TGGCCA 1 cut(s) 942
MseI TTAA 7 cut(s) 1341, 1581, 1703, 1812, 1827, 2067, 2306
MslI CAYNNNNRTG 1 cut(s) 2334
Msp20I TGGCCA 1 cut(s) 942
MspA1I CMGCKG 2 cut(s) 1215, 1613
MspI CCGG 7 cut(s) 245, 704, 815, 1556, 2511, 2572, 2636
MspR9I CCNGG 5 cut(s) 704, 815, 885, 2636, 2637
MunI CAATTG 2 cut(s) 1229, 1989
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 1 cut(s) 1083
NciI CCSGG 4 cut(s) 704, 815, 2636, 2637
NdeI CATATG 2 cut(s) 1105, 2530
NdeII GATC 6 cut(s) 452, 782, 2152, 2458, 2728, 2935
NlaIII CATG 9 cut(s) 157, 1205, 2041, 2333, 2423, 2456, 2540, 2779, 2853
NmuCI GTSAC 1 cut(s) 2100
NsbI TGCGCA 1 cut(s) 138
NspI RCATGY 2 cut(s) 2041, 2853
PaeI GCATGC 1 cut(s) 2041
PaeR7I CTCGAG 1 cut(s) 2732
PagI TCATGA 2 cut(s) 1201, 2452
PceI AGGCCT 1 cut(s) 2811
PfeI GAWTC 3 cut(s) 1306, 2416, 2601
PleI GAGTC 3 cut(s) 1171, 2314, 2474
PpsI GAGTC 3 cut(s) 1171, 2314, 2474
PpuMI RGGWCCY 1 cut(s) 51
PshAI GACNNNNGTC 1 cut(s) 2098
Psp124BI GAGCTC 1 cut(s) 1021
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 883
PspEI GGTNACC 1 cut(s) 2118
PspFI CCCAGC 7 cut(s) 487, 511, 535, 607, 679, 1151, 1215
PspGI CCWGG 1 cut(s) 883
PspPI GGNCC 7 cut(s) 51, 354, 1072, 1247, 2554, 2632, 2888
PspPPI RGGWCCY 1 cut(s) 51
PstNI CAGNNNCTG 5 cut(s) 749, 1256, 1943, 2363, 2917
PsuI RGATCY 2 cut(s) 452, 782
PvuII CAGCTG 1 cut(s) 1613
RseI CAYNNNNRTG 1 cut(s) 2334
SacI GAGCTC 1 cut(s) 1021
SaqAI TTAA 7 cut(s) 1341, 1581, 1703, 1812, 1827, 2067, 2306
Sau3AI GATC 6 cut(s) 452, 782, 2152, 2458, 2728, 2935
Sau96I GGNCC 7 cut(s) 51, 354, 1072, 1247, 2554, 2632, 2888
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 3 cut(s) 1172, 2314, 2474
ScrFI CCNGG 5 cut(s) 704, 815, 885, 2636, 2637
SduI GDGCHC 4 cut(s) 883, 1000, 1021, 2245
SfaNI GCATC 7 cut(s) 2200, 2306, 2700, 2711, 2904, 2953, 3034
SfcI CTRYAG 2 cut(s) 1485, 1923
Sfr274I CTCGAG 1 cut(s) 2732
SinI GGWCC 4 cut(s) 51, 354, 1247, 2632
SlaI CTCGAG 1 cut(s) 2732
SmaI CCCGGG 1 cut(s) 2637
SmiMI CAYNNNNRTG 1 cut(s) 2334
SmlI CTYRAG 3 cut(s) 192, 1330, 2732
SmoI CTYRAG 3 cut(s) 192, 1330, 2732
SpeI ACTAGT 1 cut(s) 1457
SphI GCATGC 1 cut(s) 2041
SseBI AGGCCT 1 cut(s) 2811
SsiI CCGC 8 cut(s) 633, 978, 1083, 1213, 1496, 2110, 2207, 2410
SspMI CTAG 6 cut(s) 36, 285, 831, 1143, 1458, 2348
SstI GAGCTC 1 cut(s) 1021
StuI AGGCCT 1 cut(s) 2811
StyD4I CCNGG 5 cut(s) 702, 813, 883, 2634, 2635
StyI CCWWGG 2 cut(s) 2156, 2202
TaaI ACNGT 6 cut(s) 30, 1489, 1823, 1902, 2013, 2268
TaiI ACGT 1 cut(s) 2875
TaqI TCGA 7 cut(s) 764, 836, 1265, 2092, 2318, 2599, 2733
TatI WGTACW 5 cut(s) 119, 386, 902, 1930, 2772
TauI GCSGC 1 cut(s) 980
TfiI GAWTC 3 cut(s) 1306, 2416, 2601
Tru1I TTAA 7 cut(s) 1341, 1581, 1703, 1812, 1827, 2067, 2306
Tru9I TTAA 7 cut(s) 1341, 1581, 1703, 1812, 1827, 2067, 2306
TscAI CASTG 7 cut(s) 427, 1048, 1141, 1720, 2273, 2331, 2860
TseFI GTSAC 1 cut(s) 2100
Tsp45I GTSAC 1 cut(s) 2100
TspDTI ATGAA 9 cut(s) 303, 816, 972, 1218, 1344, 1419, 1533, 2196, 2441
TspGWI ACGGA 1 cut(s) 999
TspMI CCCGGG 1 cut(s) 2635
TspRI CASTG 7 cut(s) 427, 1048, 1141, 1720, 2273, 2331, 2860
VpaK11BI GGWCC 4 cut(s) 51, 354, 1247, 2632
XapI RAATTY 5 cut(s) 1875, 2028, 2343, 2448, 2948
XceI RCATGY 2 cut(s) 2041, 2853
XcmI CCANNNNNNNNNTGG 3 cut(s) 62, 2265, 2441
XhoI CTCGAG 1 cut(s) 2732
XmaI CCCGGG 1 cut(s) 2635
XmiI GTMKAC 2 cut(s) 1415, 2049
XspI CTAG 6 cut(s) 36, 285, 831, 1143, 1458, 2348
ZraI GACGTC 1 cut(s) 2873
ZrmI AGTACT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.