MD15G1310800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004173
Physical Location & Seq
Forward (+)
31237566 .. 31238207
642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1310800.v1.1.491

Sequence Viewer

Length: 642 bp
ATGACACCGGAGGTAGTGCTGCAGGTCGGCCTTCTGATCCTAACCCTAGCGATGTTCTTCGCCATACACAACCTCTCCAGAAACGTCCTGACCAAGCTCCGAACCAACAACCGAGCCAACCTCCAATCGACCCGCCACTTCGTCCACGGGTCGCATCTCCTGACCCGAGCCCGATCCACTCCTCACAAGGCCCAATCTCAGGCCCACGCCAAAAACGCCCTGACCGAGGCCGAGAAGGCGCTGTCTCTGTCGCCCAGGGACCCGGGCCCACACATCCTCAAAGCCCTGTCGCTCGATCTCTTGGGGCACAAAACGTCGGCGCTGAAATCGCTCGACGCGGCTCTGTCGCCGCCTTGCGTGAAGTCGCTCTCGGAGAAGGAGCGCGGGGACGCTTTGGTGAAGAGGGCCGAGTTGAAGCTCGGGCTGAACCGGAGGCGCCGGGTGGACTCGGCGGTTGAAGACCTGGTGGATGCGGTCAAGCTGGGTCGGGCGGAGGGGGATGCGACGGCATTTTGTCTTTTGGGTCAGTGTTACGAGTGGAAGGGCATGAGGGATGAAGCCATAGACGCATTTGAGCGGGCCTTGACGGTTGATTCCGGGTCGGATTTGGCCAGTCAAGGCTTGGATCGCTTAGGATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_2 TPR_8

Protein Analysis

214

Amino Acids

23.19

Weight (kDa)

9.4

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_1 PF00515 168 - 199 7.2e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 435
AccBSI CCGCTC 1 cut(s) 577
AccII CGCG 2 cut(s) 338, 384
AciI CCGC 8 cut(s) 133, 338, 350, 384, 452, 473, 491, 577
AclWI GGATC 4 cut(s) 31, 168, 630, 633
AcoI YGGCCR 1 cut(s) 609
AcyI GRCGYC 1 cut(s) 436
AfiI CCNNNNNNNGG 2 cut(s) 199, 226
AgsI TTSAA 2 cut(s) 415, 458
AjnI CCWGG 2 cut(s) 254, 462
AluBI AGCT 3 cut(s) 97, 418, 481
AluI AGCT 3 cut(s) 97, 418, 481
Alw26I GTCTC 1 cut(s) 249
AlwI GGATC 4 cut(s) 31, 168, 630, 633
Ama87I CYCGRG 3 cut(s) 165, 262, 419
AoxI GGCC 8 cut(s) 28, 189, 201, 228, 265, 405, 579, 609
ApaI GGGCCC 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 19
AspLEI GCGC 4 cut(s) 241, 322, 384, 438
AspS9I GGNCC 7 cut(s) 190, 202, 259, 265, 266, 405, 579
AsuC2I CCSGG 4 cut(s) 263, 264, 440, 598
AsuHPI GGTGA 1 cut(s) 409
AvaI CYCGRG 3 cut(s) 165, 262, 419
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 2 cut(s) 269, 309
BalI TGGCCA 1 cut(s) 611
BamHI GGATCC 1 cut(s) 635
BanI GGYRCC 1 cut(s) 435
BanII GRGCYC 2 cut(s) 172, 269
BbsI GAAGAC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 6
BceAI ACGGC 1 cut(s) 522
BciT130I CCWGG 2 cut(s) 256, 464
BcnI CCSGG 4 cut(s) 263, 264, 440, 598
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 2 cut(s) 47, 640
BfmI CTRYAG 1 cut(s) 20
BfoI RGCGCY 3 cut(s) 242, 323, 439
BfuAI ACCTGC 1 cut(s) 13
BglI GCCNNNNNGGC 1 cut(s) 236
BisI GCNGC 3 cut(s) 20, 339, 350
BlsI GCNGC 3 cut(s) 21, 340, 351
Bme1390I CCNGG 6 cut(s) 256, 263, 264, 440, 464, 598
Bme18I GGWCC 1 cut(s) 259
BmeT110I CYCGRG 3 cut(s) 165, 262, 419
BmgT120I GGNCC 7 cut(s) 190, 202, 259, 265, 266, 405, 579
BmiI GGNNCC 5 cut(s) 260, 261, 267, 437, 637
BmrFI CCNGG 6 cut(s) 256, 263, 264, 440, 464, 598
BmsI GCATC 3 cut(s) 163, 460, 490
BpiI GAAGAC 1 cut(s) 465
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 61
Bpu10I CCTNAGC 1 cut(s) 631
BpuMI CCSGG 4 cut(s) 263, 264, 440, 598
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 5 cut(s) 145, 225, 254, 255, 262
BsaWI WCCGGW 2 cut(s) 7, 429
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 226
Bse1I ACTGG 1 cut(s) 612
BseBI CCWGG 2 cut(s) 256, 464
BseDI CCNNGG 5 cut(s) 145, 225, 254, 255, 262
BseGI GGATG 4 cut(s) 273, 475, 505, 559
BseLI CCNNNNNNNGG 2 cut(s) 199, 226
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 612
BseRI GAGGAG 1 cut(s) 171
BseSI GKGCMC 2 cut(s) 269, 309
BseXI GCAGC 1 cut(s) 6
BseYI CCCAGC 1 cut(s) 481
Bsh1236I CGCG 2 cut(s) 338, 384
BshFI GGCC 8 cut(s) 30, 191, 203, 230, 267, 407, 581, 611
BshNI GGYRCC 1 cut(s) 435
BsiHKCI CYCGRG 3 cut(s) 165, 262, 419
BsiSI CCGG 5 cut(s) 8, 263, 430, 439, 597
BslFI GGGAC 2 cut(s) 272, 401
BslI CCNNNNNNNGG 2 cut(s) 199, 226
BsmAI GTCTC 1 cut(s) 249
BsmFI GGGAC 2 cut(s) 272, 401
BsnI GGCC 8 cut(s) 30, 191, 203, 230, 267, 407, 581, 611
BsoBI CYCGRG 3 cut(s) 165, 262, 419
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 3 cut(s) 172, 269, 309
Bsp143I GATC 5 cut(s) 36, 173, 295, 625, 635
BspACI CCGC 8 cut(s) 133, 338, 350, 384, 452, 473, 491, 577
BspANI GGCC 8 cut(s) 30, 191, 203, 230, 267, 407, 581, 611
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 2 cut(s) 338, 384
BspLI GGNNCC 5 cut(s) 260, 261, 267, 437, 637
BspMAI CTGCAG 1 cut(s) 24
BspMI ACCTGC 1 cut(s) 13
BspPI GGATC 4 cut(s) 31, 168, 630, 633
BspT107I GGYRCC 1 cut(s) 435
BsrBI CCGCTC 1 cut(s) 577
BsrI ACTGG 1 cut(s) 612
BssECI CCNNGG 5 cut(s) 145, 225, 254, 255, 262
BssMI GATC 5 cut(s) 36, 173, 295, 625, 635
BssNI GRCGYC 1 cut(s) 436
Bst2UI CCWGG 2 cut(s) 256, 464
Bst4CI ACNGT 1 cut(s) 589
Bst6I CTCTTC 1 cut(s) 395
BstACI GRCGYC 1 cut(s) 436
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 2 cut(s) 198, 631
BstDSI CCRYGG 1 cut(s) 145
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 4 cut(s) 273, 475, 505, 559
BstFNI CGCG 2 cut(s) 338, 384
BstH2I RGCGCY 3 cut(s) 242, 323, 439
BstHHI GCGC 4 cut(s) 241, 322, 384, 438
BstKTI GATC 5 cut(s) 39, 176, 298, 628, 638
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 5 cut(s) 36, 173, 295, 625, 635
BstMWI GCNNNNNNNGC 5 cut(s) 215, 236, 328, 566, 627
BstNI CCWGG 2 cut(s) 256, 464
BstSCI CCNGG 6 cut(s) 254, 261, 262, 438, 462, 596
BstSFI CTRYAG 1 cut(s) 20
BstSLI GKGCMC 2 cut(s) 269, 309
BstUI CGCG 2 cut(s) 338, 384
BstV1I GCAGC 1 cut(s) 6
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 1 cut(s) 635
BstYI RGATCY 1 cut(s) 635
BsuRI GGCC 8 cut(s) 30, 191, 203, 230, 267, 407, 581, 611
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 65
BtsCI GGATG 4 cut(s) 273, 475, 505, 559
BtsIMutI CAGTG 1 cut(s) 533
BveI ACCTGC 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 579
CfoI GCGC 4 cut(s) 241, 322, 384, 438
Cfr13I GGNCC 7 cut(s) 190, 202, 259, 265, 266, 405, 579
Cfr9I CCCGGG 1 cut(s) 262
CseI GACGC 3 cut(s) 344, 398, 575
CsiI ACCWGGT 1 cut(s) 462
CviAII CATG 1 cut(s) 547
DdeI CTNAG 2 cut(s) 198, 631
DinI GGCGCC 1 cut(s) 437
DpnI GATC 5 cut(s) 38, 175, 297, 627, 637
DpnII GATC 5 cut(s) 36, 173, 295, 625, 635
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
EciI GGCGGA 1 cut(s) 506
Eco24I GRGCYC 2 cut(s) 172, 269
Eco47I GGWCC 1 cut(s) 259
Eco88I CYCGRG 3 cut(s) 165, 262, 419
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 254, 462
EcoT38I GRGCYC 2 cut(s) 172, 269
EgeI GGCGCC 1 cut(s) 437
EheI GGCGCC 1 cut(s) 437
FaeI CATG 1 cut(s) 550
FaiI YATR 3 cut(s) 65, 548, 563
FaqI GGGAC 2 cut(s) 272, 401
FatI CATG 1 cut(s) 546
FauI CCCGC 3 cut(s) 140, 377, 570
Fnu4HI GCNGC 3 cut(s) 20, 339, 350
FokI GGATG 4 cut(s) 260, 482, 512, 566
FriOI GRGCYC 2 cut(s) 172, 269
Fsp4HI GCNGC 3 cut(s) 20, 339, 350
FspBI CTAG 2 cut(s) 47, 640
GlaI GCGC 4 cut(s) 240, 321, 383, 437
GluI GCNGC 3 cut(s) 20, 339, 350
GsaI CCCAGC 1 cut(s) 485
GsuI CTGGAG 1 cut(s) 61
HaeII RGCGCY 3 cut(s) 242, 323, 439
HaeIII GGCC 8 cut(s) 30, 191, 203, 230, 267, 407, 581, 611
HapII CCGG 5 cut(s) 8, 263, 430, 439, 597
HgaI GACGC 3 cut(s) 344, 398, 575
HhaI GCGC 4 cut(s) 241, 322, 384, 438
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 1 cut(s) 550
Hin6I GCGC 4 cut(s) 239, 320, 382, 436
HinP1I GCGC 4 cut(s) 239, 320, 382, 436
HinfI GANTC 2 cut(s) 446, 593
HpaII CCGG 5 cut(s) 8, 263, 430, 439, 597
HphI GGTGA 1 cut(s) 409
Hpy166II GTNNAC 2 cut(s) 145, 445
Hpy188I TCNGA 4 cut(s) 36, 101, 373, 604
Hpy188III TCNNGA 3 cut(s) 78, 88, 160
Hpy8I GTNNAC 2 cut(s) 145, 445
Hpy99I CGWCG 3 cut(s) 319, 338, 508
HpyAV CCTTC 4 cut(s) 41, 229, 370, 535
HpyCH4III ACNGT 1 cut(s) 589
HpyCH4IV ACGT 2 cut(s) 84, 314
HpyCH4V TGCA 1 cut(s) 22
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 236, 328, 566, 627
HpyF3I CTNAG 2 cut(s) 198, 631
HpySE526I ACGT 2 cut(s) 84, 314
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 1 cut(s) 550
HspAI GCGC 4 cut(s) 239, 320, 382, 436
KasI GGCGCC 1 cut(s) 435
KflI GGGWCCC 1 cut(s) 259
Kzo9I GATC 5 cut(s) 36, 173, 295, 625, 635
LmnI GCTCC 2 cut(s) 102, 379
Lsp1109I GCAGC 1 cut(s) 6
LweI GCATC 3 cut(s) 163, 460, 490
MabI ACCWGGT 1 cut(s) 462
MaeI CTAG 2 cut(s) 47, 640
MaeII ACGT 2 cut(s) 84, 314
MaeIII GTNAC 1 cut(s) 530
MalI GATC 5 cut(s) 38, 175, 297, 627, 637
MbiI CCGCTC 1 cut(s) 577
MboI GATC 5 cut(s) 36, 173, 295, 625, 635
MboII GAAGA 3 cut(s) 49, 412, 470
MflI RGATCY 1 cut(s) 635
MhlI GDGCHC 3 cut(s) 172, 269, 309
MlsI TGGCCA 1 cut(s) 611
MluNI TGGCCA 1 cut(s) 611
Mly113I GGCGCC 1 cut(s) 436
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 582
Mox20I TGGCCA 1 cut(s) 611
MscI TGGCCA 1 cut(s) 611
Msp20I TGGCCA 1 cut(s) 611
MspI CCGG 5 cut(s) 8, 263, 430, 439, 597
MspR9I CCNGG 6 cut(s) 256, 263, 264, 440, 464, 598
MvaI CCWGG 2 cut(s) 256, 464
MvnI CGCG 2 cut(s) 338, 384
MwoI GCNNNNNNNGC 5 cut(s) 215, 236, 328, 566, 627
NarI GGCGCC 1 cut(s) 436
NciI CCSGG 4 cut(s) 263, 264, 440, 598
NdeII GATC 5 cut(s) 36, 173, 295, 625, 635
NlaIII CATG 1 cut(s) 550
NlaIV GGNNCC 5 cut(s) 260, 261, 267, 437, 637
NmeAIII GCCGAG 3 cut(s) 256, 428, 433
PasI CCCWGGG 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 1 cut(s) 593
PkrI GCNGC 3 cut(s) 21, 340, 351
PleI GAGTC 1 cut(s) 440
PluTI GGCGCC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 440
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 2 cut(s) 254, 462
PspFI CCCAGC 1 cut(s) 481
PspGI CCWGG 2 cut(s) 254, 462
PspN4I GGNNCC 5 cut(s) 260, 261, 267, 437, 637
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 7 cut(s) 190, 202, 259, 265, 266, 405, 579
PspPPI RGGWCCY 1 cut(s) 259
PstI CTGCAG 1 cut(s) 24
PsuI RGATCY 1 cut(s) 635
SatI GCNGC 3 cut(s) 20, 339, 350
Sau3AI GATC 5 cut(s) 36, 173, 295, 625, 635
Sau96I GGNCC 7 cut(s) 190, 202, 259, 265, 266, 405, 579
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 6 cut(s) 256, 263, 264, 440, 464, 598
SduI GDGCHC 3 cut(s) 172, 269, 309
SexAI ACCWGGT 1 cut(s) 462
SfaNI GCATC 3 cut(s) 163, 460, 490
SfcI CTRYAG 1 cut(s) 20
SfoI GGCGCC 1 cut(s) 437
SinI GGWCC 1 cut(s) 259
SmaI CCCGGG 1 cut(s) 264
SsiI CCGC 8 cut(s) 133, 338, 350, 384, 452, 473, 491, 577
SspDI GGCGCC 1 cut(s) 435
SspMI CTAG 2 cut(s) 47, 640
StyD4I CCNGG 6 cut(s) 254, 261, 262, 438, 462, 596
TaaI ACNGT 1 cut(s) 589
TaiI ACGT 2 cut(s) 87, 317
TaqI TCGA 3 cut(s) 128, 294, 333
TaqII GACCGA 1 cut(s) 239
TauI GCSGC 2 cut(s) 341, 352
TfiI GAWTC 1 cut(s) 593
TscAI CASTG 1 cut(s) 533
TseI GCWGC 1 cut(s) 19
TspDTI ATGAA 1 cut(s) 570
TspMI CCCGGG 1 cut(s) 262
TspRI CASTG 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 259
XagI CCTNNNNNAGG 1 cut(s) 224
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XmaI CCCGGG 1 cut(s) 262
XspI CTAG 2 cut(s) 47, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.