Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008968

Physical Location & Seq

Genomic Coordinates Reverse (-)

5042030 .. 5042896

Gene Structure 867 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1071900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 867 bp

ATGACGTTAATACTTATTATTTTTCTCCAGAAGTTCCAGATCCTTCTACGCCCTTCTTTCTTCTTTATGTGGTCACTCTCCCCTCTTAAATCCTCCCCCTCCAAATCCCCAAACCCTCATCCTGCAATTTTCCCAAACCATTCTGATTCTTCGTCTTCCCAAATCCCTCTCAAAGTTTCTCTCTTTTTTCGTTCTCAATCCCGAAAAAACCCAGAGAATTTCATGGATTCCGAAGCTGAGTTCTCTTCCTTCTTCGACTGGCTCACGAGGAGGGATCGAGCCCGAGAGCTCTCTGTGCTCATGCCCTTAATATTAGGTGCAGCCGCGGCGGCCTCCGCCGCGACACCCGACACGGAAGATCCAGATGGAGGAAACCCCGAAAGACGAACCCCGCGGGAGCGAATAGTCCTCATCGACCCTTTCAGCCAACGCGTGGCGGTGATCGAAGGTGGTTCGGACCTGGACTCGCTGCTCCAGGGGCTGGGTGGCAAGGACGGTCAGCCGCCGGCGTCGAAGGCGTCGATCGACGCCATGCCGAGTGTGGAGATTGGCGAGGCGGATGGTGGGTCCGAGTGCGCGATTTGCTTGGAGCAGTTTGAGGTGGACGGCGTGGCGAAGGAGATGCCTTGCAATCACAGGTTTCATGGGGGTTGTATAGAGCAGTGGCTGACGCTTCATGGGTCGTGCCCGGTTTGCAGGTTTACGATGCCGGTTGAGGAGGAGGAGGTGGTAAAGAAGAGCGAAGAAACGGGCGGAGAGGGAAGGAGGGTCGACGGAGAGATTAGGGTCAGGGTGTTTGTTCACAATCGCAGGAGAGCAAGTGAGGGTCCAGATCAAGCCAGTGATGAGGATACGATGCAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009987 GO:0010026 GO:0010033 GO:0010154 GO:0010498 GO:0016043 GO:0016049 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0022412 GO:0022414 GO:0030154 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032989 GO:0033554 GO:0035966 GO:0035967 GO:0036211 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048468 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0051603 GO:0051716 GO:0051787 GO:0051788 GO:0051865 GO:0060560 GO:0061458 GO:0061630 GO:0061635 GO:0061659 GO:0065007 GO:0065008 GO:0070647 GO:0070887 GO:0071218 GO:0071310 GO:0071629 GO:0071704 GO:0071840 GO:0090376 GO:0090378 GO:0090558 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

289

Amino Acids

31.79

Weight (kDa)

5.05

Isoelectric Point (pI)

71.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	191 - 233	8.1e-15	Ring finger domain
zf-RING_11	PF17123	191 - 219	2.2e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	191 - 232	3.4e-09	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	192 - 232	1.4e-07	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	207 - 233	5.3e-06	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26800
fragaria_vesca	FvH4_4g31250
malus_domestica	MD13G1071200.v1.1 MD16G1071900.v1.1
prunus_persica	Prupe.1G275700_v2.0.a1
pyrus_communis	pycom13g06390 pycom16g06220
rosa_chinensis	RchiOBHm_Chr4g0439691
rosa_laevigata	RLG00000002010 RLG00000006246 RLG00000020345
rosa_multiflora	Rmu_sc0010226.1_g000019
rosa_roxburghii	Rroxscaffold_5G00380630
rosa_rugosa	Rorug03G0296200
rosa_samantha	Rh4AG265900 Rh4BG272000 Rh4BG383500 Rh4CG398200 Rh4DG378300 Rh5AG454500
rosa_wichuraiana	Rw2G029670 Rw4G031720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	687
AccB7I	CCANNNNNTGG	2 cut(s)	433, 481
AccI	GTMKAC	1 cut(s)	771
AccII	CGCG	5 cut(s)	326, 341, 394, 432, 578
AclWI	GGATC	3 cut(s)	34, 282, 353
AcsI	RAATTY	1 cut(s)	217
AcyI	GRCGYC	3 cut(s)	509, 518, 528
AfiI	CCNNNNNNNGG	3 cut(s)	368, 433, 481
AflIII	ACRYGT	1 cut(s)	430
AjnI	CCWGG	2 cut(s)	459, 474
AluBI	AGCT	2 cut(s)	236, 289
AluI	AGCT	2 cut(s)	236, 289
Alw21I	GWGCWC	2 cut(s)	291, 300
AlwI	GGATC	3 cut(s)	34, 282, 353
AlwNI	CAGNNNCTG	2 cut(s)	481, 667
Ama87I	CYCGRG	1 cut(s)	282
AoxI	GGCC	1 cut(s)	330
ApeKI	GCWGC	2 cut(s)	320, 469
ApoI	RAATTY	1 cut(s)	217
AspLEI	GCGC	1 cut(s)	578
AspS9I	GGNCC	3 cut(s)	457, 567, 827
AsuC2I	CCSGG	1 cut(s)	689
AsuHPI	GGTGA	1 cut(s)	451
AvaI	CYCGRG	1 cut(s)	282
AvaII	GGWCC	3 cut(s)	457, 567, 827
BaeGI	GKGCMC	1 cut(s)	689
BanII	GRGCYC	2 cut(s)	283, 291
BauI	CACGAG	1 cut(s)	265
BbsI	GAAGAC	1 cut(s)	147
Bbv12I	GWGCWC	2 cut(s)	291, 300
BbvI	GCAGC	2 cut(s)	332, 456
BccI	CCATC	2 cut(s)	359, 554
BceAI	ACGGC	1 cut(s)	622
BcgI	CGANNNNNNTGC	2 cut(s)	604, 638
BciT130I	CCWGG	2 cut(s)	461, 476
BciVI	GTATCC	1 cut(s)	844
BcnI	CCSGG	1 cut(s)	689
BfuAI	ACCTGC	1 cut(s)	687
BfuI	GTATCC	1 cut(s)	844
BglI	GCCNNNNNGGC	1 cut(s)	329
BisI	GCNGC	7 cut(s)	321, 324, 327, 330, 339, 470, 503
BlsI	GCNGC	7 cut(s)	322, 325, 328, 331, 340, 471, 504
Bme1390I	CCNGG	3 cut(s)	461, 476, 689
Bme18I	GGWCC	3 cut(s)	457, 567, 827
BmeT110I	CYCGRG	1 cut(s)	282
BmgT120I	GGNCC	3 cut(s)	457, 567, 827
BmiI	GGNNCC	2 cut(s)	568, 828
BmrFI	CCNGG	3 cut(s)	461, 476, 689
BmsI	GCATC	3 cut(s)	612, 696, 846
BpiI	GAAGAC	1 cut(s)	147
BpmI	CTGGAG	2 cut(s)	11, 458
BpuMI	CCSGG	1 cut(s)	689
BsaHI	GRCGYC	3 cut(s)	509, 518, 528
BsaJI	CCNNGG	3 cut(s)	324, 392, 475
BsaXI	ACNNNNNCTCC	2 cut(s)	805, 835
Bsc4I	CCNNNNNNNGG	3 cut(s)	368, 433, 481
Bse118I	RCCGGY	2 cut(s)	505, 709
Bse1I	ACTGG	2 cut(s)	263, 840
BseBI	CCWGG	2 cut(s)	461, 476
BseDI	CCNNGG	3 cut(s)	324, 392, 475
BseGI	GGATG	2 cut(s)	118, 565
BseLI	CCNNNNNNNGG	3 cut(s)	368, 433, 481
BseMII	CTCAG	1 cut(s)	228
BseNI	ACTGG	2 cut(s)	263, 840
BseRI	GAGGAG	4 cut(s)	283, 731, 734, 737
BseSI	GKGCMC	1 cut(s)	689
BseXI	GCAGC	2 cut(s)	332, 456
BseYI	CCCAGC	1 cut(s)	481
BsgI	GTGCAG	1 cut(s)	339
Bsh1236I	CGCG	5 cut(s)	326, 341, 394, 432, 578
Bsh1285I	CGRYCG	1 cut(s)	525
BshFI	GGCC	1 cut(s)	332
BsiEI	CGRYCG	1 cut(s)	525
BsiHKAI	GWGCWC	2 cut(s)	291, 300
BsiHKCI	CYCGRG	1 cut(s)	282
BsiSI	CCGG	3 cut(s)	506, 689, 710
BslI	CCNNNNNNNGG	3 cut(s)	368, 433, 481
BsnI	GGCC	1 cut(s)	332
BsoBI	CYCGRG	1 cut(s)	282
Bsp1286I	GDGCHC	4 cut(s)	283, 291, 300, 689
Bsp143I	GATC	6 cut(s)	39, 274, 358, 441, 522, 832
BspANI	GGCC	1 cut(s)	332
BspCNI	CTCAG	1 cut(s)	229
BspFNI	CGCG	5 cut(s)	326, 341, 394, 432, 578
BspLI	GGNNCC	2 cut(s)	568, 828
BspMI	ACCTGC	1 cut(s)	687
BspPI	GGATC	3 cut(s)	34, 282, 353
BspQI	GCTCTTC	1 cut(s)	731
BsrFI	RCCGGY	2 cut(s)	505, 709
BsrI	ACTGG	2 cut(s)	263, 840
BssAI	RCCGGY	2 cut(s)	505, 709
BssECI	CCNNGG	3 cut(s)	324, 392, 475
BssMI	GATC	6 cut(s)	39, 274, 358, 441, 522, 832
BssNI	GRCGYC	3 cut(s)	509, 518, 528
BssSI	CACGAG	1 cut(s)	265
Bst2BI	CACGAG	1 cut(s)	265
Bst2UI	CCWGG	2 cut(s)	461, 476
Bst4CI	ACNGT	1 cut(s)	497
Bst6I	CTCTTC	2 cut(s)	250, 731
BstACI	GRCGYC	3 cut(s)	509, 518, 528
BstC8I	GCNNGC	1 cut(s)	507
BstDEI	CTNAG	1 cut(s)	237
BstDSI	CCRYGG	2 cut(s)	324, 392
BstF5I	GGATG	2 cut(s)	118, 565
BstFNI	CGCG	5 cut(s)	326, 341, 394, 432, 578
BstHHI	GCGC	1 cut(s)	578
BstKTI	GATC	6 cut(s)	42, 277, 361, 444, 525, 835
BstMBI	GATC	6 cut(s)	39, 274, 358, 441, 522, 832
BstMCI	CGRYCG	1 cut(s)	525
BstMWI	GCNNNNNNNGC	9 cut(s)	295, 326, 329, 335, 338, 478, 515, 582, 693
BstNI	CCWGG	2 cut(s)	461, 476
BstSCI	CCNGG	3 cut(s)	459, 474, 687
BstSLI	GKGCMC	1 cut(s)	689
BstUI	CGCG	5 cut(s)	326, 341, 394, 432, 578
BstV1I	GCAGC	2 cut(s)	332, 456
BstV2I	GAAGAC	1 cut(s)	147
BstX2I	RGATCY	2 cut(s)	39, 358
BstYI	RGATCY	2 cut(s)	39, 358
BsuI	GTATCC	1 cut(s)	844
BsuRI	GGCC	1 cut(s)	332
BtgI	CCRYGG	2 cut(s)	324, 392
BtsCI	GGATG	2 cut(s)	118, 565
BtsI	GCAGTG	1 cut(s)	668
BtsIMutI	CAGTG	2 cut(s)	668, 847
BveI	ACCTGC	1 cut(s)	687
Cac8I	GCNNGC	1 cut(s)	507
CaiI	CAGNNNCTG	2 cut(s)	481, 667
CfoI	GCGC	1 cut(s)	578
Cfr10I	RCCGGY	2 cut(s)	505, 709
Cfr13I	GGNCC	3 cut(s)	457, 567, 827
Cfr42I	CCGCGG	2 cut(s)	327, 395
CseI	GACGC	4 cut(s)	498, 507, 536, 679
CviAII	CATG	5 cut(s)	223, 301, 532, 644, 677
DdeI	CTNAG	1 cut(s)	237
DpnI	GATC	6 cut(s)	41, 276, 360, 443, 524, 834
DpnII	GATC	6 cut(s)	39, 274, 358, 441, 522, 832
Eam1104I	CTCTTC	2 cut(s)	250, 731
EarI	CTCTTC	2 cut(s)	250, 731
EciI	GGCGGA	3 cut(s)	325, 572, 768
Ecl136II	GAGCTC	1 cut(s)	289
Eco24I	GRGCYC	2 cut(s)	283, 291
Eco47I	GGWCC	3 cut(s)	457, 567, 827
Eco53kI	GAGCTC	1 cut(s)	289
Eco88I	CYCGRG	1 cut(s)	282
EcoICRI	GAGCTC	1 cut(s)	289
EcoRII	CCWGG	2 cut(s)	459, 474
EcoT38I	GRGCYC	2 cut(s)	283, 291
FaeI	CATG	5 cut(s)	226, 304, 535, 647, 680
FaiI	YATR	7 cut(s)	68, 224, 302, 533, 645, 656, 678
FatI	CATG	5 cut(s)	222, 300, 531, 643, 676
FauI	CCCGC	2 cut(s)	387, 399
FblI	GTMKAC	1 cut(s)	771
Fnu4HI	GCNGC	7 cut(s)	321, 324, 327, 330, 339, 470, 503
FokI	GGATG	2 cut(s)	105, 572
FriOI	GRGCYC	2 cut(s)	283, 291
Fsp4HI	GCNGC	7 cut(s)	321, 324, 327, 330, 339, 470, 503
GlaI	GCGC	1 cut(s)	577
GluI	GCNGC	7 cut(s)	321, 324, 327, 330, 339, 470, 503
GsaI	CCCAGC	1 cut(s)	485
GsuI	CTGGAG	2 cut(s)	11, 458
HaeIII	GGCC	1 cut(s)	332
HapII	CCGG	3 cut(s)	506, 689, 710
HgaI	GACGC	4 cut(s)	498, 507, 536, 679
HhaI	GCGC	1 cut(s)	578
Hin1I	GRCGYC	3 cut(s)	509, 518, 528
Hin1II	CATG	5 cut(s)	226, 304, 535, 647, 680
Hin6I	GCGC	1 cut(s)	576
HinP1I	GCGC	1 cut(s)	576
HincII	GTYRAC	1 cut(s)	772
HindII	GTYRAC	1 cut(s)	772
HinfI	GANTC	3 cut(s)	146, 227, 464
HpaII	CCGG	3 cut(s)	506, 689, 710
HphI	GGTGA	1 cut(s)	451
Hpy166II	GTNNAC	4 cut(s)	604, 702, 772, 802
Hpy188I	TCNGA	4 cut(s)	145, 232, 457, 571
Hpy188III	TCNNGA	6 cut(s)	28, 37, 201, 265, 362, 830
Hpy8I	GTNNAC	4 cut(s)	604, 702, 772, 802
Hpy99I	CGWCG	4 cut(s)	514, 523, 530, 776
HpyAV	CCTTC	7 cut(s)	53, 63, 259, 440, 508, 610, 756
HpyCH4III	ACNGT	1 cut(s)	497
HpyCH4IV	ACGT	1 cut(s)	5
HpyCH4V	TGCA	5 cut(s)	125, 320, 630, 696, 859
HpyF10VI	GCNNNNNNNGC	9 cut(s)	295, 326, 329, 335, 338, 478, 515, 582, 693
HpyF3I	CTNAG	1 cut(s)	237
HpySE526I	ACGT	1 cut(s)	5
Hsp92I	GRCGYC	3 cut(s)	509, 518, 528
Hsp92II	CATG	5 cut(s)	226, 304, 535, 647, 680
HspAI	GCGC	1 cut(s)	576
KroI	GCCGGC	1 cut(s)	505
KroNI	GCCGGC	1 cut(s)	507
KspI	CCGCGG	2 cut(s)	327, 395
Kzo9I	GATC	6 cut(s)	39, 274, 358, 441, 522, 832
LguI	GCTCTTC	1 cut(s)	731
LmnI	GCTCC	3 cut(s)	397, 477, 589
Lsp1109I	GCAGC	2 cut(s)	332, 456
LweI	GCATC	3 cut(s)	612, 696, 846
MaeII	ACGT	1 cut(s)	5
MaeIII	GTNAC	1 cut(s)	72
MalI	GATC	6 cut(s)	41, 276, 360, 443, 524, 834
MboI	GATC	6 cut(s)	39, 274, 358, 441, 522, 832
MboII	GAAGA	8 cut(s)	52, 141, 147, 237, 244, 368, 748, 755
MflI	RGATCY	2 cut(s)	39, 358
MhlI	GDGCHC	4 cut(s)	283, 291, 300, 689
MluCI	AATT	2 cut(s)	126, 217
MluI	ACGCGT	1 cut(s)	430
MlyI	GAGTC	1 cut(s)	458
MreI	CGCCGGCG	1 cut(s)	505
MroNI	GCCGGC	1 cut(s)	505
MseI	TTAA	3 cut(s)	8, 87, 308
MspA1I	CMGCKG	2 cut(s)	326, 394
MspI	CCGG	3 cut(s)	506, 689, 710
MspR9I	CCNGG	3 cut(s)	461, 476, 689
MvaI	CCWGG	2 cut(s)	461, 476
MvnI	CGCG	5 cut(s)	326, 341, 394, 432, 578
MwoI	GCNNNNNNNGC	9 cut(s)	295, 326, 329, 335, 338, 478, 515, 582, 693
NaeI	GCCGGC	1 cut(s)	507
NciI	CCSGG	1 cut(s)	689
NdeII	GATC	6 cut(s)	39, 274, 358, 441, 522, 832
NgoMIV	GCCGGC	1 cut(s)	505
NlaIII	CATG	5 cut(s)	226, 304, 535, 647, 680
NlaIV	GGNNCC	2 cut(s)	568, 828
NmeAIII	GCCGAG	1 cut(s)	561
NmuCI	GTSAC	1 cut(s)	72
PciSI	GCTCTTC	1 cut(s)	731
PcsI	WCGNNNNNNNCGW	1 cut(s)	518
PdiI	GCCGGC	1 cut(s)	507
PfeI	GAWTC	2 cut(s)	146, 227
PflMI	CCANNNNNTGG	2 cut(s)	433, 481
PkrI	GCNGC	7 cut(s)	322, 325, 328, 331, 340, 471, 504
Ple19I	CGATCG	1 cut(s)	525
PleI	GAGTC	1 cut(s)	458
PpsI	GAGTC	1 cut(s)	458
Psp124BI	GAGCTC	1 cut(s)	291
Psp6I	CCWGG	2 cut(s)	459, 474
PspFI	CCCAGC	1 cut(s)	481
PspGI	CCWGG	2 cut(s)	459, 474
PspN4I	GGNNCC	2 cut(s)	568, 828
PspPI	GGNCC	3 cut(s)	457, 567, 827
PstNI	CAGNNNCTG	2 cut(s)	481, 667
PsuI	RGATCY	2 cut(s)	39, 358
PvuI	CGATCG	1 cut(s)	525
SacI	GAGCTC	1 cut(s)	291
SacII	CCGCGG	2 cut(s)	327, 395
SalI	GTCGAC	1 cut(s)	770
SapI	GCTCTTC	1 cut(s)	731
SaqAI	TTAA	3 cut(s)	8, 87, 308
SatI	GCNGC	7 cut(s)	321, 324, 327, 330, 339, 470, 503
Sau3AI	GATC	6 cut(s)	39, 274, 358, 441, 522, 832
Sau96I	GGNCC	3 cut(s)	457, 567, 827
SchI	GAGTC	1 cut(s)	458
ScrFI	CCNGG	3 cut(s)	461, 476, 689
SduI	GDGCHC	4 cut(s)	283, 291, 300, 689
SfaNI	GCATC	3 cut(s)	612, 696, 846
Sfr303I	CCGCGG	2 cut(s)	327, 395
SgrAI	CRCCGGYG	1 cut(s)	505
SgrBI	CCGCGG	2 cut(s)	327, 395
SinI	GGWCC	3 cut(s)	457, 567, 827
Sse9I	AATT	2 cut(s)	126, 217
SspI	AATATT	1 cut(s)	312
SstI	GAGCTC	1 cut(s)	291
StyD4I	CCNGG	3 cut(s)	459, 474, 687
TaaI	ACNGT	1 cut(s)	497
TaiI	ACGT	1 cut(s)	8
TaqI	TCGA	8 cut(s)	255, 277, 414, 444, 512, 521, 525, 771
TasI	AATT	2 cut(s)	126, 217
TauI	GCSGC	5 cut(s)	326, 329, 332, 341, 505
TfiI	GAWTC	2 cut(s)	146, 227
Tru1I	TTAA	3 cut(s)	8, 87, 308
Tru9I	TTAA	3 cut(s)	8, 87, 308
TscAI	CASTG	2 cut(s)	668, 847
TseFI	GTSAC	1 cut(s)	72
TseI	GCWGC	2 cut(s)	320, 469
Tsp45I	GTSAC	1 cut(s)	72
TspDTI	ATGAA	3 cut(s)	211, 632, 665
TspGWI	ACGGA	2 cut(s)	368, 789
TspRI	CASTG	2 cut(s)	668, 847
Van91I	CCANNNNNTGG	2 cut(s)	433, 481
VpaK11BI	GGWCC	3 cut(s)	457, 567, 827
XapI	RAATTY	1 cut(s)	217
XcmI	CCANNNNNNNNNTGG	2 cut(s)	482, 538
XmiI	GTMKAC	1 cut(s)	771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.