Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006660

Physical Location & Seq

Genomic Coordinates Reverse (-)

9499795 .. 9502710

Gene Structure 2916 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1130200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGGTCATGGCCTCGGTGCAGCAGGTGACCCTGATCCCCGTTGACGGCGACAGTAACGGCGTTGACGCCCGCAACCCCACGTCAGCCGACACCGGTGGTGATGACTCCAGCAAGGCGCCGAGGCTGCCACGCTGGACGCGGCAGGAGATTCTCGTGCTGATAAACGGTAAGCGGTATGCGGAGAGCCGGGGCGGGGTGCGGGCACCCAGGTCGGACTTCGGGTCGGGTCAGTTGGAGCCCAAATGGGCGGCGGTGTCGAACTATTGTAAGAAGCACGGGGTGAGCCGGGAGCCGGTCCAGTGCCGGAAGCGGTGGAGCAATTTGGCGGGGGACTTCAAGAAGATCAGGGAGTGGGAGGTGCAGAGGAAGGACGAAACGGAGTCGTATTGGGTGATGAGGAACGACCTGAGGAGAGAGAGGAAGCTGCCGGGGTTTTTCGATAAGGAAGTTTACGATATAATGGAGGCGGTTCCAGGTACTCCGATTACGCTGGCTTTGCCGGGTCCGAACCGGGTGGAGTCCGAGGAGGTGAAGGAGGGGACGGCGGAGGAGGCTGAGAACTTGTTCGATAGTATACAGAGGCCGACTGAGGAGGGGTTGTTTTCGGATGACGAGTCTGGTCGGAGCCCCGAAAAGGAAGTCCGGTTCAAGGAGGGTCCGGGGATTTCAGCTCCCTTGCCAATATCAGAGAAGGCGTACAAACCAACACCACAAGGACATCAAGAACCTCAAGCACATCAAGGACAAGGTGCAGCAGGCCCAAAACAGCCAGCTTCGATCCCAGAGATTGGATCTACCTCTCAAGACCGGAAGCGGAAGCGTTTTGCAATGGAAGGAGACGAGGAAGGATCCACTCTGGAGAACCAGTTGATTGATGTCATGGAAAGGAACGGCAAAATGCTGGGTGACTACCTCCATGCTCAGAACTCCCATTCTCAACTTGACCGGGAGCAGAGGAAAGAGCATTCCGATAACCTGGTTGCCGTTCTTAACAAGCTTGCAGATGCATTCATGCGAATCGCTGAGAAATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002237 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0042221 GO:0042802 GO:0042803 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0071216 GO:0071219 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-bind_4

Protein Analysis

345

Amino Acids

38.52

Weight (kDa)

5.73

Isoelectric Point (pI)

44.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-bind_4	PF13837	44 - 132	1.3e-10	Myb/SANT-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006660)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33550
fragaria_vesca	FvH4_4g22720 FvH4_4g22720
malus_domestica	MD13G1130000.v1.1 MD16G1130200.v1.1
prunus_persica	Prupe.1G216100_v2.0.a1
pyrus_communis	pycom13g11310 pycom16g11090
rosa_chinensis	RchiOBHm_Chr2g0112661 RchiOBHm_Chr4g0428941
rosa_laevigata	RLG00000007112
rosa_multiflora	Rmu_sc0000816.1_g000016 Rmu_sc0001700.1_g000023 Rmu_sc0007311.1_g000003
rosa_roxburghii	Rroxscaffold_3G00266460 Rroxscaffold_4G00298040 Rroxscaffold_5G00370610
rosa_rugosa	Rorug04G0230900 Rorug04G0230900
rosa_samantha	Rh1DG133600 Rh3CG251300 Rh4BG293200 Rh4CG309100 Rh4DG290300 Rh6CG208200
rosa_wichuraiana	Rw4G024910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	13
Acc36I	ACCTGC	1 cut(s)	13
AccB1I	GGYRCC	2 cut(s)	115, 202
AccB7I	CCANNNNNTGG	1 cut(s)	788
AccI	GTMKAC	1 cut(s)	574
AccII	CGCG	1 cut(s)	139
AclWI	GGATC	5 cut(s)	28, 772, 799, 843, 856
AcyI	GRCGYC	2 cut(s)	66, 116
AdeI	CACNNNGTG	1 cut(s)	280
AfaI	GTAC	2 cut(s)	478, 698
AfiI	CCNNNNNNNGG	6 cut(s)	44, 193, 292, 634, 649, 788
AgeI	ACCGGT	1 cut(s)	92
AgsI	TTSAA	2 cut(s)	337, 649
AhdI	GACNNNNNGTC	1 cut(s)	220
AjiI	CACGTC	1 cut(s)	81
AjnI	CCWGG	3 cut(s)	206, 472, 975
AloI	GAACNNNNNNTCC	2 cut(s)	629, 661
AluBI	AGCT	4 cut(s)	424, 671, 773, 997
AluI	AGCT	4 cut(s)	424, 671, 773, 997
Alw26I	GTCTC	1 cut(s)	831
AlwI	GGATC	5 cut(s)	28, 772, 799, 843, 856
AoxI	GGCC	3 cut(s)	9, 581, 757
ApeKI	GCWGC	4 cut(s)	19, 124, 424, 752
ArsI	GACNNNNNNTTYG	2 cut(s)	624, 656
AsiGI	ACCGGT	1 cut(s)	92
Asp700I	GAANNNNTTC	1 cut(s)	563
AspLEI	GCGC	1 cut(s)	118
AspS9I	GGNCC	4 cut(s)	295, 503, 656, 758
AsuC2I	CCSGG	7 cut(s)	188, 287, 429, 501, 512, 660, 947
AsuHPI	GGTGA	6 cut(s)	37, 110, 292, 403, 541, 917
AvaII	GGWCC	3 cut(s)	295, 503, 656
AxyI	CCTNAGG	1 cut(s)	407
BaeGI	GKGCMC	1 cut(s)	205
BamHI	GGATCC	1 cut(s)	848
BanI	GGYRCC	2 cut(s)	115, 202
BanII	GRGCYC	2 cut(s)	240, 629
BauI	CACGAG	1 cut(s)	152
BbvI	GCAGC	4 cut(s)	31, 111, 411, 764
BceAI	ACGGC	5 cut(s)	61, 73, 558, 907, 968
BcgI	CGANNNNNNTGC	2 cut(s)	192, 226
BciT130I	CCWGG	3 cut(s)	208, 474, 977
BcnI	CCSGG	7 cut(s)	188, 287, 429, 501, 512, 660, 947
BcoDI	GTCTC	1 cut(s)	831
BfoI	RGCGCY	1 cut(s)	119
BfuAI	ACCTGC	1 cut(s)	13
BisI	GCNGC	6 cut(s)	20, 125, 140, 249, 425, 753
BlsI	GCNGC	6 cut(s)	21, 126, 141, 250, 426, 754
Bme18I	GGWCC	3 cut(s)	295, 503, 656
BmeRI	GACNNNNNGTC	1 cut(s)	220
BmgBI	CACGTC	1 cut(s)	81
BmgT120I	GGNCC	4 cut(s)	295, 503, 656, 758
BmiI	GGNNCC	9 cut(s)	117, 204, 237, 291, 471, 504, 626, 657, 850
BmsI	GCATC	1 cut(s)	994
BpmI	CTGGAG	2 cut(s)	91, 878
BpuEI	CTTGAG	2 cut(s)	714, 786
BpuMI	CCSGG	7 cut(s)	188, 287, 429, 501, 512, 660, 947
BsaHI	GRCGYC	2 cut(s)	66, 116
BsaJI	CCNNGG	7 cut(s)	12, 119, 187, 206, 428, 522, 659
BsaWI	WCCGGW	3 cut(s)	92, 642, 807
BsaXI	ACNNNNNCTCC	4 cut(s)	584, 614, 851, 881
Bsc4I	CCNNNNNNNGG	6 cut(s)	44, 193, 292, 634, 649, 788
Bse118I	RCCGGY	2 cut(s)	92, 292
Bse1I	ACTGG	2 cut(s)	298, 865
Bse21I	CCTNAGG	1 cut(s)	407
Bse3DI	GCAATG	1 cut(s)	834
BseBI	CCWGG	3 cut(s)	208, 474, 977
BseDI	CCNNGG	7 cut(s)	12, 119, 187, 206, 428, 522, 659
BseGI	GGATG	1 cut(s)	613
BseLI	CCNNNNNNNGG	6 cut(s)	44, 193, 292, 634, 649, 788
BseMI	GCAATG	1 cut(s)	834
BseMII	CTCAG	5 cut(s)	398, 546, 579, 935, 1014
BseNI	ACTGG	2 cut(s)	298, 865
BseRI	GAGGAG	4 cut(s)	424, 539, 563, 605
BseSI	GKGCMC	1 cut(s)	205
BseXI	GCAGC	4 cut(s)	31, 111, 411, 764
BseYI	CCCAGC	1 cut(s)	901
BsgI	GTGCAG	3 cut(s)	38, 380, 771
Bsh1236I	CGCG	1 cut(s)	139
BshFI	GGCC	3 cut(s)	11, 583, 759
BshNI	GGYRCC	2 cut(s)	115, 202
BshTI	ACCGGT	1 cut(s)	92
BslFI	GGGAC	2 cut(s)	344, 553
BslI	CCNNNNNNNGG	6 cut(s)	44, 193, 292, 634, 649, 788
BsmAI	GTCTC	1 cut(s)	831
BsmBI	CGTCTC	1 cut(s)	831
BsmFI	GGGAC	2 cut(s)	344, 553
BsmI	GAATGC	2 cut(s)	964, 1007
BsnI	GGCC	3 cut(s)	11, 583, 759
Bsp1286I	GDGCHC	3 cut(s)	205, 240, 629
Bsp143I	GATC	5 cut(s)	33, 342, 777, 791, 848
BspANI	GGCC	3 cut(s)	11, 583, 759
BspCNI	CTCAG	5 cut(s)	399, 547, 580, 934, 1015
BspFNI	CGCG	1 cut(s)	139
BspLI	GGNNCC	9 cut(s)	117, 204, 237, 291, 471, 504, 626, 657, 850
BspMI	ACCTGC	1 cut(s)	13
BspPI	GGATC	5 cut(s)	28, 772, 799, 843, 856
BspT107I	GGYRCC	2 cut(s)	115, 202
BsrDI	GCAATG	1 cut(s)	834
BsrFI	RCCGGY	2 cut(s)	92, 292
BsrI	ACTGG	2 cut(s)	298, 865
BssAI	RCCGGY	2 cut(s)	92, 292
BssECI	CCNNGG	7 cut(s)	12, 119, 187, 206, 428, 522, 659
BssMI	GATC	5 cut(s)	33, 342, 777, 791, 848
BssNAI	GTATAC	1 cut(s)	575
BssNI	GRCGYC	2 cut(s)	66, 116
BssSI	CACGAG	1 cut(s)	152
Bst1107I	GTATAC	1 cut(s)	575
Bst2BI	CACGAG	1 cut(s)	152
Bst2UI	CCWGG	3 cut(s)	208, 474, 977
Bst4CI	ACNGT	2 cut(s)	53, 167
BstACI	GRCGYC	2 cut(s)	66, 116
BstC8I	GCNNGC	6 cut(s)	70, 201, 492, 757, 771, 999
BstDEI	CTNAG	5 cut(s)	407, 555, 588, 921, 1023
BstEII	GGTNACC	1 cut(s)	25
BstF5I	GGATG	1 cut(s)	613
BstFNI	CGCG	1 cut(s)	139
BstH2I	RGCGCY	1 cut(s)	119
BstHHI	GCGC	1 cut(s)	118
BstKTI	GATC	5 cut(s)	36, 345, 780, 794, 851
BstMAI	GTCTC	1 cut(s)	831
BstMBI	GATC	5 cut(s)	33, 342, 777, 791, 848
BstMWI	GCNNNNNNNGC	3 cut(s)	124, 496, 551
BstNI	CCWGG	3 cut(s)	208, 474, 977
BstPI	GGTNACC	1 cut(s)	25
BstSLI	GKGCMC	1 cut(s)	205
BstUI	CGCG	1 cut(s)	139
BstV1I	GCAGC	4 cut(s)	31, 111, 411, 764
BstX2I	RGATCY	2 cut(s)	791, 848
BstYI	RGATCY	2 cut(s)	791, 848
BstZ17I	GTATAC	1 cut(s)	575
Bsu36I	CCTNAGG	1 cut(s)	407
BsuRI	GGCC	3 cut(s)	11, 583, 759
BtrI	CACGTC	1 cut(s)	81
BtsCI	GGATG	1 cut(s)	613
BtsIMutI	CAGTG	1 cut(s)	305
BveI	ACCTGC	1 cut(s)	13
Cac8I	GCNNGC	6 cut(s)	70, 201, 492, 757, 771, 999
CfoI	GCGC	1 cut(s)	118
Cfr10I	RCCGGY	2 cut(s)	92, 292
Cfr13I	GGNCC	4 cut(s)	295, 503, 656, 758
CseI	GACGC	2 cut(s)	74, 145
CsiI	ACCWGGT	1 cut(s)	975
Csp6I	GTAC	2 cut(s)	477, 697
CspAI	ACCGGT	1 cut(s)	92
CviAII	CATG	4 cut(s)	7, 880, 917, 1012
CviQI	GTAC	2 cut(s)	477, 697
DdeI	CTNAG	5 cut(s)	407, 555, 588, 921, 1023
DinI	GGCGCC	1 cut(s)	117
DpnI	GATC	5 cut(s)	35, 344, 779, 793, 850
DpnII	GATC	5 cut(s)	33, 342, 777, 791, 848
DraIII	CACNNNGTG	1 cut(s)	280
DriI	GACNNNNNGTC	1 cut(s)	220
Eam1105I	GACNNNNNGTC	1 cut(s)	220
EciI	GGCGGA	1 cut(s)	560
Eco24I	GRGCYC	2 cut(s)	240, 629
Eco47I	GGWCC	3 cut(s)	295, 503, 656
Eco81I	CCTNAGG	1 cut(s)	407
Eco91I	GGTNACC	1 cut(s)	25
EcoO65I	GGTNACC	1 cut(s)	25
EcoRII	CCWGG	3 cut(s)	206, 472, 975
EcoT22I	ATGCAT	1 cut(s)	1009
EcoT38I	GRGCYC	2 cut(s)	240, 629
EgeI	GGCGCC	1 cut(s)	117
EheI	GGCGCC	1 cut(s)	117
Esp3I	CGTCTC	1 cut(s)	831
FaeI	CATG	4 cut(s)	10, 883, 920, 1015
FaiI	YATR	7 cut(s)	8, 177, 458, 575, 881, 918, 1013
FaqI	GGGAC	2 cut(s)	344, 553
FatI	CATG	4 cut(s)	6, 879, 916, 1011
FauI	CCCGC	4 cut(s)	77, 185, 192, 319
FblI	GTMKAC	1 cut(s)	574
Fnu4HI	GCNGC	6 cut(s)	20, 125, 140, 249, 425, 753
FokI	GGATG	1 cut(s)	620
FriOI	GRGCYC	2 cut(s)	240, 629
Fsp4HI	GCNGC	6 cut(s)	20, 125, 140, 249, 425, 753
GlaI	GCGC	1 cut(s)	117
GluI	GCNGC	6 cut(s)	20, 125, 140, 249, 425, 753
GsaI	CCCAGC	1 cut(s)	905
GsuI	CTGGAG	2 cut(s)	91, 878
HaeII	RGCGCY	1 cut(s)	119
HaeIII	GGCC	3 cut(s)	11, 583, 759
HgaI	GACGC	2 cut(s)	74, 145
HhaI	GCGC	1 cut(s)	118
Hin1I	GRCGYC	2 cut(s)	66, 116
Hin1II	CATG	4 cut(s)	10, 883, 920, 1015
Hin6I	GCGC	1 cut(s)	116
HinP1I	GCGC	1 cut(s)	116
HincII	GTYRAC	2 cut(s)	43, 64
HindII	GTYRAC	2 cut(s)	43, 64
HindIII	AAGCTT	1 cut(s)	995
HinfI	GANTC	6 cut(s)	104, 148, 380, 518, 614, 1017
HphI	GGTGA	6 cut(s)	37, 110, 292, 403, 541, 917
Hpy166II	GTNNAC	4 cut(s)	43, 64, 451, 575
Hpy188I	TCNGA	9 cut(s)	214, 483, 507, 523, 607, 624, 688, 924, 970
Hpy188III	TCNNGA	4 cut(s)	337, 722, 803, 857
Hpy8I	GTNNAC	4 cut(s)	43, 64, 451, 575
HpyAV	CCTTC	5 cut(s)	361, 526, 685, 827, 839
HpyCH4III	ACNGT	2 cut(s)	53, 167
HpyCH4IV	ACGT	1 cut(s)	80
HpyCH4V	TGCA	6 cut(s)	19, 361, 752, 827, 1001, 1007
HpyF10VI	GCNNNNNNNGC	3 cut(s)	124, 496, 551
HpyF3I	CTNAG	5 cut(s)	407, 555, 588, 921, 1023
HpySE526I	ACGT	1 cut(s)	80
Hsp92I	GRCGYC	2 cut(s)	66, 116
Hsp92II	CATG	4 cut(s)	10, 883, 920, 1015
HspAI	GCGC	1 cut(s)	116
KasI	GGCGCC	1 cut(s)	115
Kzo9I	GATC	5 cut(s)	33, 342, 777, 791, 848
LmnI	GCTCC	6 cut(s)	235, 289, 315, 624, 676, 949
Lsp1109I	GCAGC	4 cut(s)	31, 111, 411, 764
LweI	GCATC	1 cut(s)	994
MabI	ACCWGGT	1 cut(s)	975
MaeII	ACGT	1 cut(s)	80
MaeIII	GTNAC	3 cut(s)	25, 53, 905
MalI	GATC	5 cut(s)	35, 344, 779, 793, 850
MboI	GATC	5 cut(s)	33, 342, 777, 791, 848
MboII	GAAGA	1 cut(s)	352
MflI	RGATCY	2 cut(s)	791, 848
MhlI	GDGCHC	3 cut(s)	205, 240, 629
MluCI	AATT	2 cut(s)	319, 1028
Mly113I	GGCGCC	1 cut(s)	116
MlyI	GAGTC	4 cut(s)	98, 389, 527, 623
MmeI	TCCRAC	3 cut(s)	192, 213, 602
Mph1103I	ATGCAT	1 cut(s)	1009
MroXI	GAANNNNTTC	1 cut(s)	563
MseI	TTAA	1 cut(s)	990
Mva1269I	GAATGC	2 cut(s)	964, 1007
MvaI	CCWGG	3 cut(s)	208, 474, 977
MvnI	CGCG	1 cut(s)	139
MwoI	GCNNNNNNNGC	3 cut(s)	124, 496, 551
NarI	GGCGCC	1 cut(s)	116
NciI	CCSGG	7 cut(s)	188, 287, 429, 501, 512, 660, 947
NdeII	GATC	5 cut(s)	33, 342, 777, 791, 848
NlaIII	CATG	4 cut(s)	10, 883, 920, 1015
NlaIV	GGNNCC	9 cut(s)	117, 204, 237, 291, 471, 504, 626, 657, 850
NmeAIII	GCCGAG	1 cut(s)	144
NmuCI	GTSAC	2 cut(s)	25, 905
NsiI	ATGCAT	1 cut(s)	1009
PaqCI	CACCTGC	1 cut(s)	13
PctI	GAATGC	2 cut(s)	964, 1007
PdmI	GAANNNNTTC	1 cut(s)	563
PfeI	GAWTC	2 cut(s)	148, 1017
PflMI	CCANNNNNTGG	1 cut(s)	788
PinAI	ACCGGT	1 cut(s)	92
PkrI	GCNGC	6 cut(s)	21, 126, 141, 250, 426, 754
PleI	GAGTC	4 cut(s)	98, 388, 526, 622
PluTI	GGCGCC	1 cut(s)	119
PpsI	GAGTC	4 cut(s)	98, 388, 526, 622
Psp6I	CCWGG	3 cut(s)	206, 472, 975
PspEI	GGTNACC	1 cut(s)	25
PspFI	CCCAGC	1 cut(s)	901
PspGI	CCWGG	3 cut(s)	206, 472, 975
PspN4I	GGNNCC	9 cut(s)	117, 204, 237, 291, 471, 504, 626, 657, 850
PspPI	GGNCC	4 cut(s)	295, 503, 656, 758
PsuI	RGATCY	2 cut(s)	791, 848
RsaI	GTAC	2 cut(s)	478, 698
RsaNI	GTAC	2 cut(s)	477, 697
SaqAI	TTAA	1 cut(s)	990
SatI	GCNGC	6 cut(s)	20, 125, 140, 249, 425, 753
Sau3AI	GATC	5 cut(s)	33, 342, 777, 791, 848
Sau96I	GGNCC	4 cut(s)	295, 503, 656, 758
SchI	GAGTC	4 cut(s)	98, 389, 527, 623
SduI	GDGCHC	3 cut(s)	205, 240, 629
SexAI	ACCWGGT	1 cut(s)	975
SfaNI	GCATC	1 cut(s)	994
SfoI	GGCGCC	1 cut(s)	117
SgrAI	CRCCGGYG	1 cut(s)	92
SinI	GGWCC	3 cut(s)	295, 503, 656
SmlI	CTYRAG	2 cut(s)	729, 801
SmoI	CTYRAG	2 cut(s)	729, 801
Sse9I	AATT	2 cut(s)	319, 1028
SspDI	GGCGCC	1 cut(s)	115
TaaI	ACNGT	2 cut(s)	53, 167
TaiI	ACGT	1 cut(s)	83
TaqI	TCGA	4 cut(s)	257, 438, 567, 776
TasI	AATT	2 cut(s)	319, 1028
TauI	GCSGC	2 cut(s)	142, 251
TfiI	GAWTC	2 cut(s)	148, 1017
Tru1I	TTAA	1 cut(s)	990
Tru9I	TTAA	1 cut(s)	990
TscAI	CASTG	1 cut(s)	305
TseFI	GTSAC	2 cut(s)	25, 905
TseI	GCWGC	4 cut(s)	19, 124, 424, 752
Tsp45I	GTSAC	2 cut(s)	25, 905
TspDTI	ATGAA	1 cut(s)	1000
TspGWI	ACGGA	1 cut(s)	392
TspRI	CASTG	1 cut(s)	305
Van91I	CCANNNNNTGG	1 cut(s)	788
VpaK11BI	GGWCC	3 cut(s)	295, 503, 656
XmiI	GTMKAC	1 cut(s)	574
XmnI	GAANNNNTTC	1 cut(s)	563
Zsp2I	ATGCAT	1 cut(s)	1009

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.