Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002167

Physical Location & Seq

Genomic Coordinates Reverse (-)

13897822 .. 13901103

Gene Structure 3282 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1167000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGGCCAGAAGCTCCGATAATCCGTCGTTAGTTCAGAGAACCCATCACAATCAACAAAATGACGACCTATCACAACCCGTTCTCGAACCGCAAACCAATCACACTTCCTCCGCCAACCCCATTCGCTACGTGCTCCGCGAGCAGCGCTTTATCTTCGTCTTCATCGGCATCGCCATCGCCGTTTTCTACTTCACCACCTTCCCGGTACCAAACCCACAATTAGAGTCCGCACAGAACTCAGGTTTTGAATCCGGGTCGAGTTTTGCCGGGCAGGGTATTGATCTGCCGGCCAACCCGGCCCGGAGAGTGCTGTTGGAGACGCGGAATGTGGGCGGGAAGGTGCCGCTGGGGGTGAAGAAGAAGAACATGAGGATTGTTGTGACGGGTGGGGCCGGGTTCGTGGGGAGCCACCTCGTGGACCGTCTGATCGCGAGAGGGGACAGCGTGATCGTGCTTGATAATTTTTTCACTGGGAGGAAGGAGAATTTGGTGCACCACTTCGGGAACCCGAAGTTCGAGCTGATTCGACACGACGTCGTCGAGCCGATTCTGCTGGAAGTTGACCAGATCTACCACCTCGCTTGCCCGGCATCCCCTGTGCATTACAAGCTTCACAAGACGAATGTGGTGGGAACACTGAACATGCTTGGACTGGCAAAAAGGGTTGGCGCACGCTTCTTGCTCACAAGCACCAGTGAGGTTTATGGTGACCCTTTGCAGCACCCTCAGGTCGAGTCCTACTGGGGCAATGTCAACCCCATCGGTGTAAGGAGCTGCTACGATGAGGGAAAACGCACAGCAGAGACTCTGACCATGGACTACCACAGAGGGCTTGGCATTGAGGTGAGGATTGCTCGGATTTTCAATACTTATGGACCGCGTATGTGCATTGATGATGGCCGGGTTGTTAGCAATTTTGTAGCTCAGGCATTAAGGAAGGAGCCATTGACAGTTTATGGAGATGGCAAGCAAACCAGAAGTTTCCAATACGTTTCTGACTTGGTAGAGGGTCTAATGCGGCTGATGGAAGGAAATCATGTGGGACCTTTCAACCTTGGCAACCCTGGCGAATTCACCATGCTAGAGCTTGCTCAGGTTGTGCAAGACACAATAGATCCAAATGCAAAAATAGAGTTCAGACCCAACACAGAAGACGACCCACACAAGAGGAAGCCGGACATTTCGAAGGCGAAAGAGCTTCTGGGGTGGGAGCCCACCGTGTCCCTCCGCAAGGGGCTGCCGCTCATGGTTAACGACTTCCGGCAGCGTATTTTTGGTGACTCCAAGGTCAATGATGCGGGCTCCGCTATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009987 GO:0016043 GO:0016829 GO:0016830 GO:0016831 GO:0022607 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043933 GO:0044085 GO:0044424 GO:0044464 GO:0046983 GO:0048037 GO:0048040 GO:0050662 GO:0051259 GO:0051262 GO:0051287 GO:0065003 GO:0070403 GO:0071840 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

GDP_Man_Dehyd

Protein Analysis

438

Amino Acids

48.71

Weight (kDa)

8.31

Isoelectric Point (pI)

34.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	123 - 382	1.5e-08	RmlD substrate binding domain
Epimerase	PF01370	125 - 353	4.9e-43	NAD dependent epimerase/dehydratase family
3Beta_HSD	PF01073	126 - 273	6.2e-11	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	127 - 414	2.3e-50	GDP-mannose 4,6 dehydratase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47650 AT2G47650 AT3G62830 AT3G62830
fragaria_vesca	FvH4_2g25720 FvH4_2g25720 FvH4_2g25720 FvH4_4g18410
malus_domestica	MD00G1128000.v1.1 MD03G1288200.v1.1 MD11G1309900.v1.1 MD16G1167000.v1.1
prunus_persica	Prupe.1G134000_v2.0.a1 Prupe.8G261400_v2.0.a1 Prupe.8G261400_v2.0.a1
pyrus_communis	pycom16g14100
rosa_chinensis	RchiOBHm_Chr4g0422861 RchiOBHm_Chr6g0294001 RchiOBHm_Chr6g0294011
rosa_laevigata	RLG00000007599 RLG00000011908
rosa_multiflora	Rmu_co8291503.1_g000001 Rmu_sc0000830.1_g000041 Rmu_sc0002321.1_g000009 Rmu_sc0003941.1_g000011 Rmu_sc0004849.1_g000008 Rmu_sc0005126.1_g000004 Rmu_sc0007876.1_g000002
rosa_roxburghii	Rroxscaffold_5G00364720 Rroxscaffold_7G00173730
rosa_rugosa	Rorug04G0181900 Rorug06G0240000
rosa_samantha	Rh4AG242300 Rh4BG245900 Rh4CG256200 Rh4DG240900 Rh6AG353100 Rh6BG360000 Rh6CG367300 Rh6DG353700 Rh6DG353800
rosa_wichuraiana	Rw4G020870 Rw6G030760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1286
AatII	GACGTC	1 cut(s)	537
Acc65I	GGTACC	1 cut(s)	205
AccB1I	GGYRCC	2 cut(s)	205, 340
AccB7I	CCANNNNNTGG	1 cut(s)	415
AccBSI	CCGCTC	1 cut(s)	1243
AccII	CGCG	4 cut(s)	138, 322, 431, 880
AclWI	GGATC	1 cut(s)	1109
AcoI	YGGCCR	3 cut(s)	3, 288, 898
AcsI	RAATTY	2 cut(s)	484, 1070
AcyI	GRCGYC	1 cut(s)	534
AdeI	CACNNNGTG	1 cut(s)	415
AfaI	GTAC	1 cut(s)	207
AfeI	AGCGCT	1 cut(s)	146
AfiI	CCNNNNNNNGG	2 cut(s)	415, 1231
AgsI	TTSAA	3 cut(s)	248, 865, 1051
AhdI	GACNNNNNGTC	1 cut(s)	533
AjnI	CCWGG	1 cut(s)	1063
AjuI	GAANNNNNNNTTGG	2 cut(s)	470, 502
AluBI	AGCT	7 cut(s)	12, 520, 610, 774, 923, 1087, 1198
AluI	AGCT	7 cut(s)	12, 520, 610, 774, 923, 1087, 1198
Alw21I	GWGCWC	2 cut(s)	135, 495
Alw26I	GTCTC	2 cut(s)	311, 797
Alw44I	GTGCAC	1 cut(s)	491
AlwI	GGATC	1 cut(s)	1109
Aor51HI	AGCGCT	1 cut(s)	146
AoxI	GGCC	5 cut(s)	3, 288, 297, 390, 898
ApaLI	GTGCAC	1 cut(s)	491
ApeKI	GCWGC	5 cut(s)	142, 718, 774, 1237, 1264
ApoI	RAATTY	2 cut(s)	484, 1070
Asp718I	GGTACC	1 cut(s)	205
AspLEI	GCGC	2 cut(s)	147, 671
AspS9I	GGNCC	5 cut(s)	298, 390, 418, 875, 1043
AsuC2I	CCSGG	8 cut(s)	203, 253, 268, 296, 301, 394, 587, 902
AsuHPI	GGTGA	6 cut(s)	184, 364, 719, 856, 1066, 1289
AsuII	TTCGAA	1 cut(s)	1184
AvaII	GGWCC	3 cut(s)	418, 875, 1043
AxyI	CCTNAGG	1 cut(s)	726
BaeGI	GKGCMC	1 cut(s)	495
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	2 cut(s)	205, 340
BanII	GRGCYC	2 cut(s)	1215, 1304
BauI	CACGAG	1 cut(s)	413
BbsI	GAAGAC	2 cut(s)	151, 1158
Bbv12I	GWGCWC	2 cut(s)	135, 495
BbvI	GCAGC	5 cut(s)	154, 730, 761, 1224, 1276
BccI	CCATC	6 cut(s)	51, 182, 767, 890, 956, 1018
BceAI	ACGGC	1 cut(s)	164
BcgI	CGANNNNNNTGC	2 cut(s)	157, 191
BciT130I	CCWGG	1 cut(s)	1065
BcnI	CCSGG	8 cut(s)	203, 253, 268, 296, 301, 394, 587, 902
BcoDI	GTCTC	2 cut(s)	311, 797
BfaI	CTAG	1 cut(s)	1082
BfoI	RGCGCY	1 cut(s)	148
BglI	GCCNNNNNGGC	1 cut(s)	296
BglII	AGATCT	1 cut(s)	567
BisI	GCNGC	8 cut(s)	143, 344, 719, 775, 1019, 1238, 1241, 1265
BlsI	GCNGC	8 cut(s)	144, 345, 720, 776, 1020, 1239, 1242, 1266
Bme1390I	CCNGG	9 cut(s)	203, 253, 268, 296, 301, 394, 587, 902, 1065
Bme18I	GGWCC	3 cut(s)	418, 875, 1043
BmeRI	GACNNNNNGTC	1 cut(s)	533
BmgT120I	GGNCC	5 cut(s)	298, 390, 418, 875, 1043
BmiI	GGNNCC	9 cut(s)	207, 342, 391, 407, 506, 942, 1044, 1212, 1303
BmrFI	CCNGG	9 cut(s)	203, 253, 268, 296, 301, 394, 587, 902, 1065
BmrI	ACTGGG	2 cut(s)	480, 751
BmsI	GCATC	3 cut(s)	177, 599, 1285
BmuI	ACTGGG	2 cut(s)	480, 751
BpiI	GAAGAC	2 cut(s)	151, 1158
BplI	GAGNNNNNCTC	2 cut(s)	838, 870
Bpu10I	CCTNAGC	2 cut(s)	924, 1092
Bpu14I	TTCGAA	1 cut(s)	1184
BpuMI	CCSGG	8 cut(s)	203, 253, 268, 296, 301, 394, 587, 902
BsaAI	YACGTR	1 cut(s)	130
BsaHI	GRCGYC	1 cut(s)	534
BsaJI	CCNNGG	4 cut(s)	813, 1054, 1063, 1284
BsaXI	ACNNNNNCTCC	2 cut(s)	92, 122
Bsc4I	CCNNNNNNNGG	2 cut(s)	415, 1231
Bse118I	RCCGGY	1 cut(s)	286
Bse1I	ACTGG	4 cut(s)	475, 657, 693, 746
Bse21I	CCTNAGG	1 cut(s)	726
Bse3DI	GCAATG	1 cut(s)	754
BseBI	CCWGG	1 cut(s)	1065
BseDI	CCNNGG	4 cut(s)	813, 1054, 1063, 1284
BseGI	GGATG	1 cut(s)	590
BseLI	CCNNNNNNNGG	2 cut(s)	415, 1231
BseMI	GCAATG	1 cut(s)	754
BseMII	CTCAG	4 cut(s)	252, 740, 938, 1106
BseNI	ACTGG	4 cut(s)	475, 657, 693, 746
BseSI	GKGCMC	1 cut(s)	495
BseXI	GCAGC	5 cut(s)	154, 730, 761, 1224, 1276
BseYI	CCCAGC	1 cut(s)	346
Bsh1236I	CGCG	4 cut(s)	138, 322, 431, 880
BshFI	GGCC	5 cut(s)	5, 290, 299, 392, 900
BshNI	GGYRCC	2 cut(s)	205, 340
BsiHKAI	GWGCWC	2 cut(s)	135, 495
BslFI	GGGAC	3 cut(s)	452, 1056, 1207
BslI	CCNNNNNNNGG	2 cut(s)	415, 1231
BsmAI	GTCTC	2 cut(s)	311, 797
BsmBI	CGTCTC	1 cut(s)	311
BsmFI	GGGAC	3 cut(s)	452, 1056, 1207
BsnI	GGCC	5 cut(s)	5, 290, 299, 392, 900
Bsp119I	TTCGAA	1 cut(s)	1184
Bsp1286I	GDGCHC	4 cut(s)	135, 495, 1215, 1304
Bsp143I	GATC	5 cut(s)	280, 426, 447, 567, 1114
Bsp19I	CCATGG	1 cut(s)	813
Bsp68I	TCGCGA	1 cut(s)	431
BspANI	GGCC	5 cut(s)	5, 290, 299, 392, 900
BspCNI	CTCAG	4 cut(s)	251, 739, 937, 1105
BspFNI	CGCG	4 cut(s)	138, 322, 431, 880
BspLI	GGNNCC	9 cut(s)	207, 342, 391, 407, 506, 942, 1044, 1212, 1303
BspPI	GGATC	1 cut(s)	1109
BspT104I	TTCGAA	1 cut(s)	1184
BspT107I	GGYRCC	2 cut(s)	205, 340
BsrBI	CCGCTC	1 cut(s)	1243
BsrDI	GCAATG	1 cut(s)	754
BsrFI	RCCGGY	1 cut(s)	286
BsrI	ACTGG	4 cut(s)	475, 657, 693, 746
BssAI	RCCGGY	1 cut(s)	286
BssECI	CCNNGG	4 cut(s)	813, 1054, 1063, 1284
BssMI	GATC	5 cut(s)	280, 426, 447, 567, 1114
BssNI	GRCGYC	1 cut(s)	534
BssSI	CACGAG	1 cut(s)	413
BssT1I	CCWWGG	3 cut(s)	813, 1054, 1284
Bst2BI	CACGAG	1 cut(s)	413
Bst2UI	CCWGG	1 cut(s)	1065
Bst4CI	ACNGT	3 cut(s)	422, 952, 1219
BstACI	GRCGYC	1 cut(s)	534
BstBAI	YACGTR	1 cut(s)	130
BstBI	TTCGAA	1 cut(s)	1184
BstC8I	GCNNGC	7 cut(s)	140, 288, 583, 673, 968, 1089, 1300
BstDEI	CTNAG	4 cut(s)	238, 726, 924, 1092
BstDSI	CCRYGG	1 cut(s)	813
BstEII	GGTNACC	1 cut(s)	707
BstENI	CCTNNNNNAGG	1 cut(s)	1229
BstF5I	GGATG	1 cut(s)	590
BstFNI	CGCG	4 cut(s)	138, 322, 431, 880
BstH2I	RGCGCY	1 cut(s)	148
BstHHI	GCGC	2 cut(s)	147, 671
BstKTI	GATC	5 cut(s)	283, 429, 450, 570, 1117
BstMAI	GTCTC	2 cut(s)	311, 797
BstMBI	GATC	5 cut(s)	280, 426, 447, 567, 1114
BstMWI	GCNNNNNNNGC	8 cut(s)	139, 144, 296, 550, 587, 607, 1065, 1304
BstNI	CCWGG	1 cut(s)	1065
BstNSI	RCATGY	1 cut(s)	646
BstPI	GGTNACC	1 cut(s)	707
BstSCI	CCNGG	9 cut(s)	201, 251, 266, 294, 299, 392, 585, 900, 1063
BstSLI	GKGCMC	1 cut(s)	495
BstUI	CGCG	4 cut(s)	138, 322, 431, 880
BstV1I	GCAGC	5 cut(s)	154, 730, 761, 1224, 1276
BstV2I	GAAGAC	2 cut(s)	151, 1158
BstX2I	RGATCY	2 cut(s)	567, 1114
BstYI	RGATCY	2 cut(s)	567, 1114
Bsu36I	CCTNAGG	1 cut(s)	726
BsuRI	GGCC	5 cut(s)	5, 290, 299, 392, 900
BtgI	CCRYGG	1 cut(s)	813
BtgZI	GCGATG	2 cut(s)	154, 160
BtsCI	GGATG	1 cut(s)	590
BtsIMutI	CAGTG	3 cut(s)	468, 635, 700
BtuMI	TCGCGA	1 cut(s)	431
Cac8I	GCNNGC	7 cut(s)	140, 288, 583, 673, 968, 1089, 1300
CfoI	GCGC	2 cut(s)	147, 671
Cfr10I	RCCGGY	1 cut(s)	286
Cfr13I	GGNCC	5 cut(s)	298, 390, 418, 875, 1043
CseI	GACGC	1 cut(s)	328
Csp6I	GTAC	1 cut(s)	206
CspCI	CAANNNNNGTGG	2 cut(s)	563, 598
CviAII	CATG	6 cut(s)	367, 643, 814, 1037, 1078, 1246
CviQI	GTAC	1 cut(s)	206
DdeI	CTNAG	4 cut(s)	238, 726, 924, 1092
DpnI	GATC	5 cut(s)	282, 428, 449, 569, 1116
DpnII	GATC	5 cut(s)	280, 426, 447, 567, 1114
DraIII	CACNNNGTG	1 cut(s)	415
DrdI	GACNNNNNNGTC	1 cut(s)	1286
DriI	GACNNNNNGTC	1 cut(s)	533
DseDI	GACNNNNNNGTC	1 cut(s)	1286
EaeI	YGGCCR	3 cut(s)	3, 288, 898
Eam1105I	GACNNNNNGTC	1 cut(s)	533
EciI	GGCGGA	1 cut(s)	100
Eco130I	CCWWGG	3 cut(s)	813, 1054, 1284
Eco24I	GRGCYC	2 cut(s)	1215, 1304
Eco47I	GGWCC	3 cut(s)	418, 875, 1043
Eco47III	AGCGCT	1 cut(s)	146
Eco81I	CCTNAGG	1 cut(s)	726
Eco91I	GGTNACC	1 cut(s)	707
EcoNI	CCTNNNNNAGG	1 cut(s)	1229
EcoO109I	RGGNCCY	1 cut(s)	1043
EcoO65I	GGTNACC	1 cut(s)	707
EcoRI	GAATTC	1 cut(s)	1070
EcoRII	CCWGG	1 cut(s)	1063
EcoT14I	CCWWGG	3 cut(s)	813, 1054, 1284
EcoT38I	GRGCYC	2 cut(s)	1215, 1304
ErhI	CCWWGG	3 cut(s)	813, 1054, 1284
Esp3I	CGTCTC	1 cut(s)	311
FaeI	CATG	6 cut(s)	370, 646, 817, 1040, 1081, 1249
FaqI	GGGAC	3 cut(s)	452, 1056, 1207
FatI	CATG	6 cut(s)	366, 642, 813, 1036, 1077, 1245
FauI	CCCGC	2 cut(s)	326, 1291
Fnu4HI	GCNGC	8 cut(s)	143, 344, 719, 775, 1019, 1238, 1241, 1265
FokI	GGATG	1 cut(s)	577
FriOI	GRGCYC	2 cut(s)	1215, 1304
Fsp4HI	GCNGC	8 cut(s)	143, 344, 719, 775, 1019, 1238, 1241, 1265
FspBI	CTAG	1 cut(s)	1082
GlaI	GCGC	2 cut(s)	146, 670
GluI	GCNGC	8 cut(s)	143, 344, 719, 775, 1019, 1238, 1241, 1265
GsaI	CCCAGC	1 cut(s)	350
HaeII	RGCGCY	1 cut(s)	148
HaeIII	GGCC	5 cut(s)	5, 290, 299, 392, 900
HgaI	GACGC	1 cut(s)	328
HhaI	GCGC	2 cut(s)	147, 671
Hin1I	GRCGYC	1 cut(s)	534
Hin1II	CATG	6 cut(s)	370, 646, 817, 1040, 1081, 1249
Hin6I	GCGC	2 cut(s)	145, 669
HinP1I	GCGC	2 cut(s)	145, 669
HincII	GTYRAC	3 cut(s)	562, 754, 1252
HindII	GTYRAC	3 cut(s)	562, 754, 1252
HindIII	AAGCTT	1 cut(s)	608
HinfI	GANTC	7 cut(s)	224, 248, 523, 547, 734, 805, 1280
HpaI	GTTAAC	1 cut(s)	1252
HphI	GGTGA	6 cut(s)	184, 364, 719, 856, 1066, 1289
Hpy166II	GTNNAC	5 cut(s)	418, 493, 562, 754, 1252
Hpy188I	TCNGA	7 cut(s)	16, 36, 426, 810, 858, 997, 1139
Hpy188III	TCNNGA	3 cut(s)	83, 430, 502
Hpy8I	GTNNAC	5 cut(s)	418, 493, 562, 754, 1252
Hpy99I	CGWCG	4 cut(s)	28, 536, 539, 542
HpyAV	CCTTC	6 cut(s)	208, 331, 472, 931, 1022, 1180
HpyCH4III	ACNGT	3 cut(s)	422, 952, 1219
HpyCH4IV	ACGT	3 cut(s)	129, 534, 990
HpyCH4V	TGCA	6 cut(s)	493, 601, 718, 888, 1102, 1124
HpyF10VI	GCNNNNNNNGC	8 cut(s)	139, 144, 296, 550, 587, 607, 1065, 1304
HpyF3I	CTNAG	4 cut(s)	238, 726, 924, 1092
HpySE526I	ACGT	3 cut(s)	129, 534, 990
Hsp92I	GRCGYC	1 cut(s)	534
Hsp92II	CATG	6 cut(s)	370, 646, 817, 1040, 1081, 1249
HspAI	GCGC	2 cut(s)	145, 669
KpnI	GGTACC	1 cut(s)	209
KroI	GCCGGC	1 cut(s)	286
KroNI	GCCGGC	1 cut(s)	288
KspAI	GTTAAC	1 cut(s)	1252
Kzo9I	GATC	5 cut(s)	280, 426, 447, 567, 1114
LmnI	GCTCC	7 cut(s)	17, 138, 405, 771, 940, 1210, 1307
Lsp1109I	GCAGC	5 cut(s)	154, 730, 761, 1224, 1276
LweI	GCATC	3 cut(s)	177, 599, 1285
MaeI	CTAG	1 cut(s)	1082
MaeII	ACGT	3 cut(s)	129, 534, 990
MaeIII	GTNAC	3 cut(s)	379, 707, 1277
MalI	GATC	5 cut(s)	282, 428, 449, 569, 1116
MbiI	CCGCTC	1 cut(s)	1243
MboI	GATC	5 cut(s)	280, 426, 447, 567, 1114
MboII	GAAGA	6 cut(s)	145, 151, 367, 370, 373, 1163
MflI	RGATCY	2 cut(s)	567, 1114
MhlI	GDGCHC	4 cut(s)	135, 495, 1215, 1304
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	5 cut(s)	218, 460, 484, 913, 1070
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	4 cut(s)	233, 743, 799, 1274
MmeI	TCCRAC	1 cut(s)	294
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	1 cut(s)	286
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	932, 1251
MslI	CAYNNNNRTG	1 cut(s)	842
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	346
MspR9I	CCNGG	9 cut(s)	203, 253, 268, 296, 301, 394, 587, 902, 1065
MvaI	CCWGG	1 cut(s)	1065
MvnI	CGCG	4 cut(s)	138, 322, 431, 880
MwoI	GCNNNNNNNGC	8 cut(s)	139, 144, 296, 550, 587, 607, 1065, 1304
NaeI	GCCGGC	1 cut(s)	288
NciI	CCSGG	8 cut(s)	203, 253, 268, 296, 301, 394, 587, 902
NcoI	CCATGG	1 cut(s)	813
NdeII	GATC	5 cut(s)	280, 426, 447, 567, 1114
NgoMIV	GCCGGC	1 cut(s)	286
NlaIII	CATG	6 cut(s)	370, 646, 817, 1040, 1081, 1249
NlaIV	GGNNCC	9 cut(s)	207, 342, 391, 407, 506, 942, 1044, 1212, 1303
NmuCI	GTSAC	3 cut(s)	379, 707, 1277
NruI	TCGCGA	1 cut(s)	431
NspI	RCATGY	1 cut(s)	646
NspV	TTCGAA	1 cut(s)	1184
PcsI	WCGNNNNNNNCGW	3 cut(s)	135, 177, 537
PdiI	GCCGGC	1 cut(s)	288
PfeI	GAWTC	3 cut(s)	248, 523, 547
PflFI	GACNNNGTC	1 cut(s)	536
PflMI	CCANNNNNTGG	1 cut(s)	415
PkrI	GCNGC	8 cut(s)	144, 345, 720, 776, 1020, 1239, 1242, 1266
PleI	GAGTC	4 cut(s)	232, 742, 799, 1274
PpsI	GAGTC	4 cut(s)	232, 742, 799, 1274
Ppu21I	YACGTR	1 cut(s)	130
PpuMI	RGGWCCY	1 cut(s)	1043
Psp5II	RGGWCCY	1 cut(s)	1043
Psp6I	CCWGG	1 cut(s)	1063
PspEI	GGTNACC	1 cut(s)	707
PspFI	CCCAGC	1 cut(s)	346
PspGI	CCWGG	1 cut(s)	1063
PspN4I	GGNNCC	9 cut(s)	207, 342, 391, 407, 506, 942, 1044, 1212, 1303
PspPI	GGNCC	5 cut(s)	298, 390, 418, 875, 1043
PspPPI	RGGWCCY	1 cut(s)	1043
PsuI	RGATCY	2 cut(s)	567, 1114
PsyI	GACNNNGTC	1 cut(s)	536
RruI	TCGCGA	1 cut(s)	431
RsaI	GTAC	1 cut(s)	207
RsaNI	GTAC	1 cut(s)	206
RseI	CAYNNNNRTG	1 cut(s)	842
SaqAI	TTAA	2 cut(s)	932, 1251
SatI	GCNGC	8 cut(s)	143, 344, 719, 775, 1019, 1238, 1241, 1265
Sau3AI	GATC	5 cut(s)	280, 426, 447, 567, 1114
Sau96I	GGNCC	5 cut(s)	298, 390, 418, 875, 1043
SchI	GAGTC	4 cut(s)	233, 743, 799, 1274
ScrFI	CCNGG	9 cut(s)	203, 253, 268, 296, 301, 394, 587, 902, 1065
SduI	GDGCHC	4 cut(s)	135, 495, 1215, 1304
SfaNI	GCATC	3 cut(s)	177, 599, 1285
SfiI	GGCCNNNNNGGCC	1 cut(s)	296
SfuI	TTCGAA	1 cut(s)	1184
SinI	GGWCC	3 cut(s)	418, 875, 1043
SmiMI	CAYNNNNRTG	1 cut(s)	842
Sse9I	AATT	5 cut(s)	218, 460, 484, 913, 1070
SspMI	CTAG	1 cut(s)	1082
StyD4I	CCNGG	9 cut(s)	201, 251, 266, 294, 299, 392, 585, 900, 1063
StyI	CCWWGG	3 cut(s)	813, 1054, 1284
TaaI	ACNGT	3 cut(s)	422, 952, 1219
TaiI	ACGT	3 cut(s)	132, 537, 993
TaqI	TCGA	7 cut(s)	84, 257, 516, 526, 540, 732, 1184
TasI	AATT	5 cut(s)	218, 460, 484, 913, 1070
TauI	GCSGC	3 cut(s)	346, 1021, 1243
TfiI	GAWTC	3 cut(s)	248, 523, 547
Tru1I	TTAA	2 cut(s)	932, 1251
Tru9I	TTAA	2 cut(s)	932, 1251
TscAI	CASTG	3 cut(s)	475, 642, 700
TseFI	GTSAC	3 cut(s)	379, 707, 1277
TseI	GCWGC	5 cut(s)	142, 718, 774, 1237, 1264
Tsp45I	GTSAC	3 cut(s)	379, 707, 1277
TspDTI	ATGAA	1 cut(s)	151
TspGWI	ACGGA	1 cut(s)	12
TspRI	CASTG	3 cut(s)	475, 642, 700
Tth111I	GACNNNGTC	1 cut(s)	536
Van91I	CCANNNNNTGG	1 cut(s)	415
VneI	GTGCAC	1 cut(s)	491
VpaK11BI	GGWCC	3 cut(s)	418, 875, 1043
XagI	CCTNNNNNAGG	1 cut(s)	1229
XapI	RAATTY	2 cut(s)	484, 1070
XceI	RCATGY	1 cut(s)	646
XcmI	CCANNNNNNNNNTGG	1 cut(s)	830
XspI	CTAG	1 cut(s)	1082
ZraI	GACGTC	1 cut(s)	535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.