Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004894

Physical Location & Seq

Genomic Coordinates Forward (+)

5206559 .. 5209674

Gene Structure 3116 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1064200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGGGGTTTCTGTTCCTGCCCATGATTCGATCAGTCCCACAATTCATCAGTTTCGCCACCTTTCTTGTCTTTGTGCTTTCTTGCACCACCAGCTTCATTGCAACAGAAGCGTATGATCCACTCGACCCGAACGGAAATATCACCATCAAATGGGATATCTTAAGCTGGACTCCTGATGGCTATGTTGCTGCTGTTACACTTTTTAACTTCCAAAAGTATCGGCACATTCAGGCACCGGGATGGTCGCTAGGGTGGACATGGGCAAAGAAGGAGGTTATATGGAACATGGTGGGAGGACAAGCCACAGAGCAAGGGGATTGTTCAAGATTCAAAACAACACTCCCCCATTGCTGTAAGAAGACTCCTACAGTTGTTGATCTATTGCCCGGAACACCTTACAATCAACAATACACAAATTGTTGCAAAGGAGGGGTACTTAGTTCATGGGTGCAAGATCCAGAAAAGGCTGTAGGTTCTTTTCAAATAAGCGTTGGTCAAGCAGGAACCACAAACAAGACCGTCAGACTGCCTAAAAACTTCACCCTGAACACACCCGGTCCTGGGTATACTTGTGCTCGCGCCAAAGTAGTCAAACCAACTAAATTTATCAGTCCGGATAAAAGGAGAGTCACTCAAGCTATGATGACATGGAATGTGACGTGCACATATTCGCAAATACTGGCTCAGAAAACTCCTACATGTTGTGTCTCGCTCTCATCGTTCTACAACGACACTATAGTGCCCTGCCCAAATTGCTCATGCGGTTGCCAGAGAAACGCAACTCATCCAGGAAGCTGTGTAGAACCAGATTCCCCCTACTTGGCTTCAGTTGTCTCAGCCTCCAACAAGAATAGCTACACGCCTCTAGTTAGATGCACTGCGCACATGTGCCCAATCCGAGTTCACTGGCATGTCAAGGTAAACTACAAACAATACTGGCGAGTGAAGGTTACAGTTACAAACTTCAATTACAGGATGAATTATTCCGATTGGAACATGGTTGTCCAACACCCAAACTTTGACAATCTGACCCAGATTTTCAGCTACAACTACAAGTCGATAACTCCTTATGCGACAATAAATGATACTGGCATGTTATGGGGAATTAAGTTCTACAATGATATTCTGATGCAAGCTGGGCCCCTTGGTAATGTACAGTCAGAGCTGCTTTTCCAAAAGGATGAAGCAACTTTCACATTTGATAAGGGTTGGGCGTTCCCTCGAAGGGTCTATTTCAACGGTGATAACTGTGTGATGCCACCTCCAGATGCCTATCCATGGTTGCCAAATTCTGGTTTCCGGCAGTATGTTTCTCTGCTCACTCTAATCATGACTTGTCTATCGACCATTGCAGTCATGTATGCTTATGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COBRA

Protein Analysis

458

Amino Acids

51.58

Weight (kDa)

8.96

Isoelectric Point (pI)

31.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
COBRA	PF04833	61 - 224	1.1e-67	COBRA-like protein
COB_C	PF25079	233 - 426	1.8e-61	COBRA family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004894)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02210 AT3G29810
fragaria_vesca	FvH4_6g46460 FvH4_6g46530
malus_domestica	MD09G1072800.v1.1 MD17G1064200.v1.1
prunus_persica	Prupe.3G250400_v2.0.a1 Prupe.3G250400_v2.0.a1
pyrus_communis	pycom12433g00030 pycom17g06430
rosa_chinensis	RchiOBHm_Chr2g0153051 RchiOBHm_Chr2g0165011 RchiOBHm_Chr2g0165171 RchiOBHm_Chr5g0001191
rosa_multiflora	Rmu_sc0001097.1_g000020 Rmu_sc0001097.1_g000043
rosa_roxburghii	Rroxscaffold_2G00085970 Rroxscaffold_2G00086120
rosa_rugosa	Rorug02G0512200 Rorug02G0512300 Rorug02G0512900
rosa_samantha	Rh2AG580300 Rh2AG581100 Rh2BG591600 Rh2BG592400 Rh2CG562100 Rh2CG563100 Rh2DG601700 Rh2DG602400
rosa_wichuraiana	Rw2G048170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	882
AccB1I	GGYRCC	1 cut(s)	232
AccB7I	CCANNNNNTGG	2 cut(s)	150, 1292
AccI	GTMKAC	1 cut(s)	566
AccII	CGCG	1 cut(s)	579
AccIII	TCCGGA	1 cut(s)	613
AciI	CCGC	1 cut(s)	762
AclWI	GGATC	2 cut(s)	110, 449
AcsI	RAATTY	2 cut(s)	602, 1288
AcuI	CTGAAG	1 cut(s)	810
AfaI	GTAC	2 cut(s)	435, 1155
AfiI	CCNNNNNNNGG	7 cut(s)	150, 560, 561, 820, 1225, 1278, 1292
AflII	CTTAAG	1 cut(s)	160
AflIII	ACRYGT	2 cut(s)	698, 885
AgsI	TTSAA	5 cut(s)	324, 331, 482, 967, 1237
AjiI	CACGTC	1 cut(s)	660
AjnI	CCWGG	2 cut(s)	559, 787
AleI	CACNNNNGTG	1 cut(s)	737
AluBI	AGCT	8 cut(s)	93, 165, 638, 795, 855, 1044, 1136, 1165
AluI	AGCT	8 cut(s)	93, 165, 638, 795, 855, 1044, 1136, 1165
Alw21I	GWGCWC	2 cut(s)	577, 665
Alw26I	GTCTC	2 cut(s)	712, 838
Alw44I	GTGCAC	1 cut(s)	661
AlwI	GGATC	2 cut(s)	110, 449
Aor13HI	TCCGGA	1 cut(s)	613
AoxI	GGCC	1 cut(s)	1139
ApaI	GGGCCC	1 cut(s)	1143
ApaLI	GTGCAC	1 cut(s)	661
ApeKI	GCWGC	2 cut(s)	188, 1165
ApoI	RAATTY	2 cut(s)	602, 1288
AspLEI	GCGC	2 cut(s)	581, 883
AspS9I	GGNCC	3 cut(s)	557, 1139, 1140
AsuC2I	CCSGG	3 cut(s)	237, 387, 555
AsuHPI	GGTGA	3 cut(s)	133, 532, 1253
AvaII	GGWCC	1 cut(s)	557
BaeGI	GKGCMC	4 cut(s)	665, 744, 893, 1143
BanI	GGYRCC	1 cut(s)	232
BanII	GRGCYC	1 cut(s)	1143
BbsI	GAAGAC	1 cut(s)	365
Bbv12I	GWGCWC	2 cut(s)	577, 665
BbvI	GCAGC	2 cut(s)	175, 1152
BccI	CCATC	3 cut(s)	152, 170, 234
BciT130I	CCWGG	2 cut(s)	561, 789
BcnI	CCSGG	3 cut(s)	237, 387, 555
BcoDI	GTCTC	2 cut(s)	712, 838
BfaI	CTAG	2 cut(s)	248, 866
BfmI	CTRYAG	3 cut(s)	366, 468, 735
BfrI	CTTAAG	1 cut(s)	160
BisI	GCNGC	2 cut(s)	189, 1166
BlsI	GCNGC	2 cut(s)	190, 1167
Bme1390I	CCNGG	5 cut(s)	237, 387, 555, 561, 789
Bme18I	GGWCC	1 cut(s)	557
BmgBI	CACGTC	1 cut(s)	660
BmgT120I	GGNCC	3 cut(s)	557, 1139, 1140
BmiI	GGNNCC	4 cut(s)	234, 505, 1141, 1142
BmrFI	CCNGG	5 cut(s)	237, 387, 555, 561, 789
BmsI	GCATC	4 cut(s)	863, 1119, 1245, 1258
BpiI	GAAGAC	1 cut(s)	365
BplI	GAGNNNNNCTC	2 cut(s)	616, 648
BpmI	CTGGAG	1 cut(s)	1248
BpuEI	CTTGAG	1 cut(s)	618
BpuMI	CCSGG	3 cut(s)	237, 387, 555
BsaBI	GATNNNNATC	1 cut(s)	1272
BsaJI	CCNNGG	3 cut(s)	560, 1144, 1277
BsaWI	WCCGGW	1 cut(s)	613
Bsc4I	CCNNNNNNNGG	7 cut(s)	150, 560, 561, 820, 1225, 1278, 1292
Bse1I	ACTGG	4 cut(s)	684, 911, 941, 1093
Bse3DI	GCAATG	3 cut(s)	96, 346, 1347
Bse8I	GATNNNNATC	1 cut(s)	1272
BseAI	TCCGGA	1 cut(s)	613
BseBI	CCWGG	2 cut(s)	561, 789
BseDI	CCNNGG	3 cut(s)	560, 1144, 1277
BseGI	GGATG	4 cut(s)	245, 784, 981, 1186
BseJI	GATNNNNATC	1 cut(s)	1272
BseLI	CCNNNNNNNGG	7 cut(s)	150, 560, 561, 820, 1225, 1278, 1292
BseMI	GCAATG	3 cut(s)	96, 346, 1347
BseMII	CTCAG	2 cut(s)	698, 849
BseNI	ACTGG	4 cut(s)	684, 911, 941, 1093
BseSI	GKGCMC	4 cut(s)	665, 744, 893, 1143
BseXI	GCAGC	2 cut(s)	175, 1152
BseYI	CCCAGC	1 cut(s)	1136
Bsh1236I	CGCG	1 cut(s)	579
BshFI	GGCC	1 cut(s)	1141
BshNI	GGYRCC	1 cut(s)	232
BsiHKAI	GWGCWC	2 cut(s)	577, 665
BsiSI	CCGG	5 cut(s)	236, 387, 555, 614, 1300
BslFI	GGGAC	1 cut(s)	20
BslI	CCNNNNNNNGG	7 cut(s)	150, 560, 561, 820, 1225, 1278, 1292
BsmAI	GTCTC	2 cut(s)	712, 838
BsmFI	GGGAC	1 cut(s)	20
BsnI	GGCC	1 cut(s)	1141
Bsp120I	GGGCCC	1 cut(s)	1139
Bsp1286I	GDGCHC	5 cut(s)	577, 665, 744, 893, 1143
Bsp13I	TCCGGA	1 cut(s)	613
Bsp1407I	TGTACA	1 cut(s)	1153
Bsp143I	GATC	4 cut(s)	29, 115, 376, 454
Bsp19I	CCATGG	1 cut(s)	1277
BspACI	CCGC	1 cut(s)	762
BspANI	GGCC	1 cut(s)	1141
BspCNI	CTCAG	2 cut(s)	697, 848
BspEI	TCCGGA	1 cut(s)	613
BspFNI	CGCG	1 cut(s)	579
BspHI	TCATGA	1 cut(s)	1329
BspLI	GGNNCC	4 cut(s)	234, 505, 1141, 1142
BspPI	GGATC	2 cut(s)	110, 449
BspT107I	GGYRCC	1 cut(s)	232
BspTI	CTTAAG	1 cut(s)	160
BsrDI	GCAATG	3 cut(s)	96, 346, 1347
BsrGI	TGTACA	1 cut(s)	1153
BsrI	ACTGG	4 cut(s)	684, 911, 941, 1093
BssECI	CCNNGG	3 cut(s)	560, 1144, 1277
BssMI	GATC	4 cut(s)	29, 115, 376, 454
BssNAI	GTATAC	1 cut(s)	567
BssT1I	CCWWGG	2 cut(s)	1144, 1277
Bst1107I	GTATAC	1 cut(s)	567
Bst2UI	CCWGG	2 cut(s)	561, 789
Bst4CI	ACNGT	6 cut(s)	370, 520, 955, 1158, 1241, 1250
BstAFI	CTTAAG	1 cut(s)	160
BstAUI	TGTACA	1 cut(s)	1153
BstC8I	GCNNGC	2 cut(s)	577, 1134
BstDEI	CTNAG	3 cut(s)	437, 684, 835
BstDSI	CCRYGG	1 cut(s)	1277
BstF5I	GGATG	4 cut(s)	245, 784, 981, 1186
BstFNI	CGCG	1 cut(s)	579
BstHHI	GCGC	2 cut(s)	581, 883
BstKTI	GATC	4 cut(s)	32, 118, 379, 457
BstMAI	GTCTC	2 cut(s)	712, 838
BstMBI	GATC	4 cut(s)	29, 115, 376, 454
BstMWI	GCNNNNNNNGC	4 cut(s)	90, 107, 753, 1138
BstNI	CCWGG	2 cut(s)	561, 789
BstNSI	RCATGY	4 cut(s)	702, 889, 914, 1096
BstSCI	CCNGG	5 cut(s)	235, 385, 553, 559, 787
BstSFI	CTRYAG	3 cut(s)	366, 468, 735
BstSLI	GKGCMC	4 cut(s)	665, 744, 893, 1143
BstUI	CGCG	1 cut(s)	579
BstV1I	GCAGC	2 cut(s)	175, 1152
BstV2I	GAAGAC	1 cut(s)	365
BstX2I	RGATCY	1 cut(s)	454
BstYI	RGATCY	1 cut(s)	454
BstZ17I	GTATAC	1 cut(s)	567
BsuRI	GGCC	1 cut(s)	1141
BtgI	CCRYGG	1 cut(s)	1277
BtrI	CACGTC	1 cut(s)	660
BtsCI	GGATG	4 cut(s)	245, 784, 981, 1186
BtsI	GCAGTG	1 cut(s)	876
BtsIMutI	CAGTG	2 cut(s)	876, 904
Cac8I	GCNNGC	2 cut(s)	577, 1134
CciI	TCATGA	1 cut(s)	1329
CfoI	GCGC	2 cut(s)	581, 883
Cfr13I	GGNCC	3 cut(s)	557, 1139, 1140
Csp6I	GTAC	2 cut(s)	434, 1154
CspCI	CAANNNNNGTGG	2 cut(s)	46, 81
CviQI	GTAC	2 cut(s)	434, 1154
DdeI	CTNAG	3 cut(s)	437, 684, 835
DpnI	GATC	4 cut(s)	31, 117, 378, 456
DpnII	GATC	4 cut(s)	29, 115, 376, 454
Eco130I	CCWWGG	2 cut(s)	1144, 1277
Eco24I	GRGCYC	1 cut(s)	1143
Eco32I	GATATC	1 cut(s)	157
Eco47I	GGWCC	1 cut(s)	557
Eco57I	CTGAAG	1 cut(s)	810
EcoO109I	RGGNCCY	1 cut(s)	1140
EcoRII	CCWGG	2 cut(s)	559, 787
EcoRV	GATATC	1 cut(s)	157
EcoT14I	CCWWGG	2 cut(s)	1144, 1277
EcoT38I	GRGCYC	1 cut(s)	1143
ErhI	CCWWGG	2 cut(s)	1144, 1277
FaqI	GGGAC	1 cut(s)	20
FblI	GTMKAC	1 cut(s)	566
Fnu4HI	GCNGC	2 cut(s)	189, 1166
FokI	GGATG	4 cut(s)	252, 771, 988, 1193
FriOI	GRGCYC	1 cut(s)	1143
Fsp4HI	GCNGC	2 cut(s)	189, 1166
FspBI	CTAG	2 cut(s)	248, 866
FspI	TGCGCA	1 cut(s)	882
GlaI	GCGC	2 cut(s)	580, 882
GluI	GCNGC	2 cut(s)	189, 1166
GsaI	CCCAGC	1 cut(s)	1140
GsuI	CTGGAG	1 cut(s)	1248
HaeIII	GGCC	1 cut(s)	1141
HapII	CCGG	5 cut(s)	236, 387, 555, 614, 1300
HhaI	GCGC	2 cut(s)	581, 883
Hin6I	GCGC	2 cut(s)	579, 881
HinP1I	GCGC	2 cut(s)	579, 881
HinfI	GANTC	6 cut(s)	25, 169, 327, 361, 627, 809
HpaII	CCGG	5 cut(s)	236, 387, 555, 614, 1300
HphI	GGTGA	3 cut(s)	133, 532, 1253
Hpy166II	GTNNAC	5 cut(s)	255, 567, 663, 904, 922
Hpy188I	TCNGA	7 cut(s)	524, 687, 899, 988, 1029, 1128, 1162
Hpy188III	TCNNGA	6 cut(s)	173, 324, 458, 614, 1265, 1330
Hpy8I	GTNNAC	5 cut(s)	255, 567, 663, 904, 922
HpyAV	CCTTC	3 cut(s)	262, 940, 1218
HpyCH4III	ACNGT	6 cut(s)	370, 520, 955, 1158, 1241, 1250
HpyCH4IV	ACGT	1 cut(s)	659
HpyCH4V	TGCA	8 cut(s)	84, 101, 423, 451, 663, 876, 1132, 1352
HpyF10VI	GCNNNNNNNGC	4 cut(s)	90, 107, 753, 1138
HpyF3I	CTNAG	3 cut(s)	437, 684, 835
HpySE526I	ACGT	1 cut(s)	659
HspAI	GCGC	2 cut(s)	579, 881
Kpn2I	TCCGGA	1 cut(s)	613
Kzo9I	GATC	4 cut(s)	29, 115, 376, 454
Lsp1109I	GCAGC	2 cut(s)	175, 1152
LweI	GCATC	4 cut(s)	863, 1119, 1245, 1258
MaeI	CTAG	2 cut(s)	248, 866
MaeII	ACGT	1 cut(s)	659
MaeIII	GTNAC	5 cut(s)	193, 628, 655, 949, 955
MalI	GATC	4 cut(s)	31, 117, 378, 456
MboI	GATC	4 cut(s)	29, 115, 376, 454
MboII	GAAGA	1 cut(s)	370
MflI	RGATCY	1 cut(s)	454
MhlI	GDGCHC	5 cut(s)	577, 665, 744, 893, 1143
MluCI	AATT	8 cut(s)	41, 415, 602, 751, 967, 979, 1104, 1288
MlyI	GAGTC	3 cut(s)	163, 355, 636
MmeI	TCCRAC	2 cut(s)	867, 1030
MnlI	CCTC	7 cut(s)	265, 287, 422, 850, 873, 1230, 1272
MroI	TCCGGA	1 cut(s)	613
MseI	TTAA	4 cut(s)	161, 204, 1107, 1372
MslI	CAYNNNNRTG	3 cut(s)	238, 737, 909
MspCI	CTTAAG	1 cut(s)	160
MspI	CCGG	5 cut(s)	236, 387, 555, 614, 1300
MspR9I	CCNGG	5 cut(s)	237, 387, 555, 561, 789
MvaI	CCWGG	2 cut(s)	561, 789
MvnI	CGCG	1 cut(s)	579
MwoI	GCNNNNNNNGC	4 cut(s)	90, 107, 753, 1138
NciI	CCSGG	3 cut(s)	237, 387, 555
NcoI	CCATGG	1 cut(s)	1277
NdeII	GATC	4 cut(s)	29, 115, 376, 454
NlaIV	GGNNCC	4 cut(s)	234, 505, 1141, 1142
NmuCI	GTSAC	2 cut(s)	628, 655
NsbI	TGCGCA	1 cut(s)	882
NspI	RCATGY	4 cut(s)	702, 889, 914, 1096
OliI	CACNNNNGTG	1 cut(s)	737
PagI	TCATGA	1 cut(s)	1329
PciI	ACATGT	2 cut(s)	698, 885
PcsI	WCGNNNNNNNCGW	1 cut(s)	716
PfeI	GAWTC	3 cut(s)	25, 327, 809
PflMI	CCANNNNNTGG	2 cut(s)	150, 1292
PfoI	TCCNGGA	1 cut(s)	787
PkrI	GCNGC	2 cut(s)	190, 1167
PleI	GAGTC	3 cut(s)	163, 355, 635
PpsI	GAGTC	3 cut(s)	163, 355, 635
PscI	ACATGT	2 cut(s)	698, 885
Psp6I	CCWGG	2 cut(s)	559, 787
PspFI	CCCAGC	1 cut(s)	1136
PspGI	CCWGG	2 cut(s)	559, 787
PspN4I	GGNNCC	4 cut(s)	234, 505, 1141, 1142
PspOMI	GGGCCC	1 cut(s)	1139
PspPI	GGNCC	3 cut(s)	557, 1139, 1140
PsuI	RGATCY	1 cut(s)	454
RsaI	GTAC	2 cut(s)	435, 1155
RsaNI	GTAC	2 cut(s)	434, 1154
RseI	CAYNNNNRTG	3 cut(s)	238, 737, 909
SaqAI	TTAA	4 cut(s)	161, 204, 1107, 1372
SatI	GCNGC	2 cut(s)	189, 1166
Sau3AI	GATC	4 cut(s)	29, 115, 376, 454
Sau96I	GGNCC	3 cut(s)	557, 1139, 1140
SchI	GAGTC	3 cut(s)	163, 355, 636
ScrFI	CCNGG	5 cut(s)	237, 387, 555, 561, 789
SduI	GDGCHC	5 cut(s)	577, 665, 744, 893, 1143
SfaNI	GCATC	4 cut(s)	863, 1119, 1245, 1258
SfcI	CTRYAG	3 cut(s)	366, 468, 735
SinI	GGWCC	1 cut(s)	557
SmiMI	CAYNNNNRTG	3 cut(s)	238, 737, 909
SmlI	CTYRAG	2 cut(s)	160, 633
SmoI	CTYRAG	2 cut(s)	160, 633
Sse9I	AATT	8 cut(s)	41, 415, 602, 751, 967, 979, 1104, 1288
SsiI	CCGC	1 cut(s)	762
SspMI	CTAG	2 cut(s)	248, 866
StyD4I	CCNGG	5 cut(s)	235, 385, 553, 559, 787
StyI	CCWWGG	2 cut(s)	1144, 1277
TaaI	ACNGT	6 cut(s)	370, 520, 955, 1158, 1241, 1250
TaiI	ACGT	1 cut(s)	662
TaqI	TCGA	5 cut(s)	28, 123, 1058, 1222, 1343
TasI	AATT	8 cut(s)	41, 415, 602, 751, 967, 979, 1104, 1288
TatI	WGTACW	1 cut(s)	1153
TfiI	GAWTC	3 cut(s)	25, 327, 809
Tru1I	TTAA	4 cut(s)	161, 204, 1107, 1372
Tru9I	TTAA	4 cut(s)	161, 204, 1107, 1372
TscAI	CASTG	2 cut(s)	883, 911
TseFI	GTSAC	2 cut(s)	628, 655
TseI	GCWGC	2 cut(s)	188, 1165
Tsp45I	GTSAC	2 cut(s)	628, 655
TspDTI	ATGAA	5 cut(s)	34, 85, 432, 992, 1197
TspGWI	ACGGA	1 cut(s)	147
TspRI	CASTG	2 cut(s)	883, 911
Van91I	CCANNNNNTGG	2 cut(s)	150, 1292
Vha464I	CTTAAG	1 cut(s)	160
VneI	GTGCAC	1 cut(s)	661
VpaK11BI	GGWCC	1 cut(s)	557
XapI	RAATTY	2 cut(s)	602, 1288
XceI	RCATGY	4 cut(s)	702, 889, 914, 1096
XmiI	GTMKAC	1 cut(s)	566
XspI	CTAG	2 cut(s)	248, 866

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.