MD17G1281100.v1.1

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002715
Physical Location & Seq
Forward (+)
34074761 .. 34076498
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1281100.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGAGATTTTACCTGCAATGCAAGGAAGCAATCTAAAAGGAGGGTTGCTGCTAAGTGCAGTGCTTCCTCTGCTGAAGCTCCTTTCAATGGCTGCCATTGGTTTTCTTCTTGCCCACCCAAAATACCAACTTGTCCCTAAACTCACTTTCACATTTCTCAGTAAGTTGGTATTTGCTGTCTTTTTACCCTGCTTAATCTTCACAAATCTAGGGCCATCAATTAGTCTAAAGAATTTTGTAAATTGGTGGTTCATTCCTGTTAATGTTGTAATAAGTACGGCAATCGGGTGTGTGTTGGGTTTGCTGGTTGCAGTGATTTGTAGGCCTCCACCTCAGTTTTTTCGATTCACCGTGATCATGACTGCGTTCGGAAACACGGGTAATCTCCCTCTTGCAATTGTTGCATCTGTTTGCCATAGTGATGATAACCCTTTCGGTACAGAAGATGACTGTACTGAGGCTGGTGTGGCATATGTTTCTTTTTCCCAATGGGTTTCTGTCATTCTTGTTTACACTCTTGTTTACCATATGATGGAGCCCCCATTGGAGTCTGCTGTTTATGCAGTTGTGGAGGAAGAGGAGAATCATGAGATTGCGGAACTGGGTTCGGATGGGAATGGGAATGGGAATGGGAATGGGAATGGGAATCGAGATGAAAGTGATCTTGCTAGGCCGCTTCTCGTTGAGGCTGAATGGCCTGGAATTGAAGATAAGGAAACTGAACATTGCAAGACACCCTTTATTGCAAGGCTTTTTAATAGCAGCAATGGTGTACTTTCACACAATAGTCTTTCGGAACTTGGTGAGGAGGAGGAGGAGGGAGGTTATCCGAGAAGTACCAAGCCTAGTAAGTGTCTGGCAGAGCCTAGGATCGTGAGGAAATTCAGATATGTTGCTTGGAGGACACCAATTCATCGCATTCTTCAGCCGCCAACAATTGCTTTTCTGTTGGCCGTTGTCATTGGCATGATTCCAACATTGAAATCTCTTGTGTTTGATGATGACCAAGTCCTTTCGTTCCTCACAGACAGCTTGGAAATTGTAGCGGAGGCCATGGTGCCTTCGGCTGTGCTTGTTCTTGGAGGAATGCTAGCTGAAGGTCCCAACAAATCCAATCTAGGAATGAGAACTACGATTGGGATTATTGTTGCAAGGCTTTTGGTACTTCCTTTAATTGGGATTGGTGTGGTTCTATTGTCGGATAAATTGAACCTTTTGGTAGCCGATGATCAATTGTATCGGTTCGTTCTTTTGCTGCAATACACCACACCTTCTGCCATATTGTTAGGAGCAGTTGCAAGCTTGAGGGGTTATGCAGTCAGAGAGGCTTCTGCTCTCCTCTTCTGGCAGCATATTGTTGCCGTCTTCTCCCTCTCGCTCTATATAATTGTCTACTTCTACATTCTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

471

Amino Acids

51.4

Weight (kDa)

5.13

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 21 - 462 4.2e-89 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 532
AccI GTMKAC 1 cut(s) 1392
AciI CCGC 4 cut(s) 596, 674, 929, 1046
AclWI GGATC 1 cut(s) 878
AcoI YGGCCR 1 cut(s) 951
AcsI RAATTY 2 cut(s) 232, 881
AcuI CTGAAG 3 cut(s) 95, 908, 1116
AfaI GTAC 6 cut(s) 277, 439, 454, 774, 838, 1164
AfiI CCNNNNNNNGG 3 cut(s) 88, 532, 1175
AgsI TTSAA 4 cut(s) 87, 707, 982, 1210
AjnI CCWGG 1 cut(s) 697
AluBI AGCT 4 cut(s) 79, 1032, 1094, 1302
AluI AGCT 4 cut(s) 79, 1032, 1094, 1302
AlwI GGATC 1 cut(s) 878
AoxI GGCC 6 cut(s) 212, 323, 671, 695, 951, 1050
ApeKI GCWGC 5 cut(s) 49, 92, 762, 1255, 1348
ApoI RAATTY 2 cut(s) 232, 881
AspA2I CCTAGG 1 cut(s) 866
AspS9I GGNCC 2 cut(s) 212, 1100
AsuHPI GGTGA 2 cut(s) 340, 815
AsuNHI GCTAGC 1 cut(s) 1090
AvaII GGWCC 1 cut(s) 1100
AvrII CCTAGG 1 cut(s) 866
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 1357
BbvI GCAGC 5 cut(s) 36, 79, 774, 1242, 1360
BccI CCATC 3 cut(s) 223, 526, 605
BceAI ACGGC 3 cut(s) 294, 938, 1346
BciT130I CCWGG 1 cut(s) 699
BclI TGATCA 2 cut(s) 354, 1228
BfaI CTAG 6 cut(s) 209, 669, 846, 867, 1091, 1118
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 7 cut(s) 50, 93, 674, 763, 929, 1256, 1349
BlnI CCTAGG 1 cut(s) 866
BlsI GCNGC 7 cut(s) 51, 94, 675, 764, 930, 1257, 1350
Bme1390I CCNGG 1 cut(s) 699
Bme18I GGWCC 1 cut(s) 1100
BmgT120I GGNCC 2 cut(s) 212, 1100
BmiI GGNNCC 3 cut(s) 537, 1059, 1102
BmrFI CCNGG 1 cut(s) 699
BmrI ACTGGG 1 cut(s) 611
BmsI GCATC 1 cut(s) 413
BmtI GCTAGC 1 cut(s) 1094
BmuI ACTGGG 1 cut(s) 611
BpiI GAAGAC 1 cut(s) 1357
BpuEI CTTGAG 1 cut(s) 1324
BsaJI CCNNGG 2 cut(s) 866, 1053
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 532, 1175
Bse1I ACTGG 1 cut(s) 606
Bse3DI GCAATG 3 cut(s) 24, 724, 772
BseBI CCWGG 1 cut(s) 699
BseDI CCNNGG 2 cut(s) 866, 1053
BseGI GGATG 1 cut(s) 616
BseLI CCNNNNNNNGG 3 cut(s) 88, 532, 1175
BseMI GCAATG 3 cut(s) 24, 724, 772
BseMII CTCAG 3 cut(s) 172, 347, 447
BseNI ACTGG 1 cut(s) 606
BseRI GAGGAG 6 cut(s) 593, 821, 824, 827, 830, 1328
BseXI GCAGC 5 cut(s) 36, 79, 774, 1242, 1360
BsgI GTGCAG 1 cut(s) 78
BshFI GGCC 6 cut(s) 214, 325, 673, 697, 953, 1052
BshNI GGYRCC 1 cut(s) 1057
BslFI GGGAC 2 cut(s) 119, 1086
BslI CCNNNNNNNGG 3 cut(s) 88, 532, 1175
BsmFI GGGAC 2 cut(s) 119, 1086
BsmI GAATGC 2 cut(s) 918, 1092
BsnI GGCC 6 cut(s) 214, 325, 673, 697, 953, 1052
Bsp1286I GDGCHC 1 cut(s) 540
Bsp143I GATC 4 cut(s) 354, 661, 870, 1228
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 4 cut(s) 596, 674, 929, 1046
BspANI GGCC 6 cut(s) 214, 325, 673, 697, 953, 1052
BspCNI CTCAG 3 cut(s) 171, 346, 448
BspHI TCATGA 2 cut(s) 357, 586
BspLI GGNNCC 3 cut(s) 537, 1059, 1102
BspMI ACCTGC 1 cut(s) 22
BspOI GCTAGC 1 cut(s) 1094
BspPI GGATC 1 cut(s) 878
BspT107I GGYRCC 1 cut(s) 1057
BsrDI GCAATG 3 cut(s) 24, 724, 772
BsrI ACTGG 1 cut(s) 606
BssECI CCNNGG 2 cut(s) 866, 1053
BssMI GATC 4 cut(s) 354, 661, 870, 1228
BssT1I CCWWGG 2 cut(s) 866, 1053
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 2 cut(s) 352, 452
Bst6I CTCTTC 2 cut(s) 570, 1346
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 1092, 1300
BstDEI CTNAG 4 cut(s) 53, 158, 333, 456
BstDSI CCRYGG 1 cut(s) 1053
BstF5I GGATG 1 cut(s) 616
BstKTI GATC 4 cut(s) 357, 664, 873, 1231
BstMBI GATC 4 cut(s) 354, 661, 870, 1228
BstMWI GCNNNNNNNGC 4 cut(s) 70, 401, 467, 560
BstNI CCWGG 1 cut(s) 699
BstSCI CCNGG 1 cut(s) 697
BstV1I GCAGC 5 cut(s) 36, 79, 774, 1242, 1360
BstV2I GAAGAC 1 cut(s) 1357
BsuRI GGCC 6 cut(s) 214, 325, 673, 697, 953, 1052
BtgI CCRYGG 1 cut(s) 1053
BtgZI GCGATG 1 cut(s) 899
BtsCI GGATG 1 cut(s) 616
BtsI GCAGTG 2 cut(s) 66, 318
BtsIMutI CAGTG 2 cut(s) 66, 318
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 1092, 1300
CciI TCATGA 2 cut(s) 357, 586
Cfr13I GGNCC 2 cut(s) 212, 1100
Csp6I GTAC 6 cut(s) 276, 438, 453, 773, 837, 1163
CviAII CATG 4 cut(s) 358, 587, 967, 1054
CviQI GTAC 6 cut(s) 276, 438, 453, 773, 837, 1163
DdeI CTNAG 4 cut(s) 53, 158, 333, 456
DpnI GATC 4 cut(s) 356, 663, 872, 1230
DpnII GATC 4 cut(s) 354, 661, 870, 1228
EaeI YGGCCR 1 cut(s) 951
Eam1104I CTCTTC 2 cut(s) 570, 1346
EarI CTCTTC 2 cut(s) 570, 1346
Eco130I CCWWGG 2 cut(s) 866, 1053
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 1100
Eco57I CTGAAG 3 cut(s) 95, 908, 1116
EcoO109I RGGNCCY 1 cut(s) 1100
EcoRII CCWGG 1 cut(s) 697
EcoT14I CCWWGG 2 cut(s) 866, 1053
EcoT38I GRGCYC 1 cut(s) 540
ErhI CCWWGG 2 cut(s) 866, 1053
FaeI CATG 4 cut(s) 361, 590, 970, 1057
FalI AAGNNNNNCTT 2 cut(s) 722, 754
FaqI GGGAC 2 cut(s) 119, 1086
FatI CATG 4 cut(s) 357, 586, 966, 1053
FauNDI CATATG 2 cut(s) 472, 528
FbaI TGATCA 2 cut(s) 354, 1228
FblI GTMKAC 1 cut(s) 1392
Fnu4HI GCNGC 7 cut(s) 50, 93, 674, 763, 929, 1256, 1349
FokI GGATG 1 cut(s) 623
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 7 cut(s) 50, 93, 674, 763, 929, 1256, 1349
FspBI CTAG 6 cut(s) 209, 669, 846, 867, 1091, 1118
GluI GCNGC 7 cut(s) 50, 93, 674, 763, 929, 1256, 1349
HaeIII GGCC 6 cut(s) 214, 325, 673, 697, 953, 1052
Hin1II CATG 4 cut(s) 361, 590, 970, 1057
HindIII AAGCTT 1 cut(s) 1300
HinfI GANTC 5 cut(s) 345, 548, 583, 646, 970
HphI GGTGA 2 cut(s) 340, 815
Hpy166II GTNNAC 4 cut(s) 511, 523, 773, 1393
Hpy188I TCNGA 7 cut(s) 371, 610, 796, 831, 887, 1201, 1322
Hpy188III TCNNGA 4 cut(s) 358, 587, 650, 874
Hpy8I GTNNAC 4 cut(s) 511, 523, 773, 1393
HpyAV CCTTC 3 cut(s) 1071, 1091, 1281
HpyCH4III ACNGT 2 cut(s) 352, 452
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 401, 467, 560
HpyF3I CTNAG 4 cut(s) 53, 158, 333, 456
Hsp92II CATG 4 cut(s) 361, 590, 970, 1057
Ksp22I TGATCA 2 cut(s) 354, 1228
Kzo9I GATC 4 cut(s) 354, 661, 870, 1228
LmnI GCTCC 3 cut(s) 84, 535, 1289
Lsp1109I GCAGC 5 cut(s) 36, 79, 774, 1242, 1360
LweI GCATC 1 cut(s) 413
MaeI CTAG 6 cut(s) 209, 669, 846, 867, 1091, 1118
MalI GATC 4 cut(s) 356, 663, 872, 1230
MboI GATC 4 cut(s) 354, 661, 870, 1228
MboII GAAGA 8 cut(s) 98, 190, 455, 587, 719, 914, 1333, 1357
MfeI CAATTG 3 cut(s) 396, 936, 1232
MhlI GDGCHC 1 cut(s) 540
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 2 cut(s) 998, 1179
MseI TTAA 4 cut(s) 194, 261, 756, 1172
MslI CAYNNNNRTG 2 cut(s) 420, 965
MspR9I CCNGG 1 cut(s) 699
MunI CAATTG 3 cut(s) 396, 936, 1232
Mva1269I GAATGC 2 cut(s) 918, 1092
MvaI CCWGG 1 cut(s) 699
MwoI GCNNNNNNNGC 4 cut(s) 70, 401, 467, 560
NcoI CCATGG 1 cut(s) 1053
NdeI CATATG 2 cut(s) 472, 528
NdeII GATC 4 cut(s) 354, 661, 870, 1228
NheI GCTAGC 1 cut(s) 1090
NlaIII CATG 4 cut(s) 361, 590, 970, 1057
NlaIV GGNNCC 3 cut(s) 537, 1059, 1102
PagI TCATGA 2 cut(s) 357, 586
PceI AGGCCT 1 cut(s) 325
PctI GAATGC 2 cut(s) 918, 1092
PfeI GAWTC 4 cut(s) 345, 583, 646, 970
PflFI GACNNNGTC 1 cut(s) 1007
PflMI CCANNNNNTGG 1 cut(s) 532
PkrI GCNGC 7 cut(s) 51, 94, 675, 764, 930, 1257, 1350
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 1100
Psp5II RGGWCCY 1 cut(s) 1100
Psp6I CCWGG 1 cut(s) 697
PspGI CCWGG 1 cut(s) 697
PspN4I GGNNCC 3 cut(s) 537, 1059, 1102
PspPI GGNCC 2 cut(s) 212, 1100
PspPPI RGGWCCY 1 cut(s) 1100
PsyI GACNNNGTC 1 cut(s) 1007
RsaI GTAC 6 cut(s) 277, 439, 454, 774, 838, 1164
RsaNI GTAC 6 cut(s) 276, 438, 453, 773, 837, 1163
RseI CAYNNNNRTG 2 cut(s) 420, 965
SaqAI TTAA 4 cut(s) 194, 261, 756, 1172
SatI GCNGC 7 cut(s) 50, 93, 674, 763, 929, 1256, 1349
Sau3AI GATC 4 cut(s) 354, 661, 870, 1228
Sau96I GGNCC 2 cut(s) 212, 1100
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 1 cut(s) 699
SduI GDGCHC 1 cut(s) 540
SfaNI GCATC 1 cut(s) 413
SinI GGWCC 1 cut(s) 1100
SmiMI CAYNNNNRTG 2 cut(s) 420, 965
SmlI CTYRAG 1 cut(s) 1303
SmoI CTYRAG 1 cut(s) 1303
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 4 cut(s) 596, 674, 929, 1046
SspMI CTAG 6 cut(s) 209, 669, 846, 867, 1091, 1118
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 697
StyI CCWWGG 2 cut(s) 866, 1053
TaaI ACNGT 2 cut(s) 352, 452
TaqI TCGA 2 cut(s) 343, 649
TatI WGTACW 2 cut(s) 452, 772
TauI GCSGC 2 cut(s) 676, 931
TfiI GAWTC 4 cut(s) 345, 583, 646, 970
Tru1I TTAA 4 cut(s) 194, 261, 756, 1172
Tru9I TTAA 4 cut(s) 194, 261, 756, 1172
TscAI CASTG 2 cut(s) 66, 318
TseI GCWGC 5 cut(s) 49, 92, 762, 1255, 1348
TspDTI ATGAA 3 cut(s) 241, 669, 902
TspRI CASTG 2 cut(s) 66, 318
Tth111I GACNNNGTC 1 cut(s) 1007
Van91I CCANNNNNTGG 1 cut(s) 532
VpaK11BI GGWCC 1 cut(s) 1100
XapI RAATTY 2 cut(s) 232, 881
XmaJI CCTAGG 1 cut(s) 866
XmiI GTMKAC 1 cut(s) 1392
XspI CTAG 6 cut(s) 209, 669, 846, 867, 1091, 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.