Rw3G027360

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002715
Physical Location & Seq
Forward (+)
38766567 .. 38769385
2819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027360.1

Sequence Viewer

Length: 1362 bp
ATGTCACCGCTATATCAAACGATGGAAAACATACTTGCTTCATTCCAAGGAACTCTAAAAGTAGAAGTGCTAAGTGCAGTAGTGCCTTTATTAATGTTCCTGTCTATGGGTCTGACTGGCTTAGTTCTTGCCCACCCAAAGTACCAACTAGTCCCCAGAGCTACTTTCACATTTCTAAGCAAGCTGGTCTTTGTTGTCTTCTTGCCCTGCTTGATCTTCACATATCTTGGCCCAACAATCACTCTCAAAAACTTGCTGCTCTGGTGGTTCATTCCTGTAAATGTTGTTCTAAGTACCGCCATTGGCTGCGTATTGGGGTTGCTGGTGGCGCTAATCTGTAGGCCTCCCCGCGACTACTTTCGATTCACCATAATAATGACTGCGTTTGGAAACACAGGTAATCTGCCCCTTGCAATTGTTACCTCTATTTGTCAAAGTGATGATAACCCTTTCGGTTCTGATTGTTACGACTATGGTGTGTCATATGTATCATTTTCCCAATGGGTTTCTGTTGTTCTCGTTTACACACTTGTTTACCATATGATGGAGCCCCCATTAAGGGATCAAAGTCTACTCGTTGAGGAAGAAGAAGATGATGAAGATGAGATATTTGAGAAAATTATGTCTGGTCCAAATTATGAAGATGAAAATCATCTGAGTAGGCCTCTTCTTGTTGAGGCTGAATGGCCTGGAATTGAAGATAAAGAAACTGAGCACTGTAAGACACCCTTTATTGCAAGACTTTTTACTACTAGAAGCTCCTCTGCTCGACTTTCAGGAACATCCAACTACAGTTCCAAGTGTTTGGCTGAGCCTAGAATGGTAAGGAAGTTCAGATCAGTAGTGGGGATGACACCAATTCATTACATTCTTCAGCCCCCAACAATTGCTTCCCTCTTGGCTATAATCATTGGGATGGTCCCCACGTTGAAATCAATTGTTTACGATGACGATGCAGTTCTTTCATTTATTACAGACAGCTTAGAAATTCTATCCGAGGCTATGGTGCCTTCAGCTATGCTTGTTCTTGGAGGATCGCTTGCTCAAGGACCAAATGAATCCAATCTCGGAGTTCGAACTACAGTTGGGATTATTATTGCAAGACTTTTTATACTTCCTTTGATTGGAATTGGTGTCATTCATTTGGCCGATAAATGGAATATTCTAATAGCCGGAGATCAAATGTTCCGGTTGGTACTTTTGCTGCAGTACACAATGCCAAGTGCCATCTTGTTAGGAGCAATTGCAAGCTTGAGGGGTTATGCAGTTAAGGAGGCTTCAGCACTGCTCTTCTGGCAGCATGTTGTTGCTGTTTTGTCCATTTCATTCTATATAATTGTCTACTTCTATATCTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

453

Amino Acids

50.38

Weight (kDa)

5.15

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 27 - 445 3.3e-86 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1006
AccB7I CCANNNNNTGG 1 cut(s) 544
AccI GTMKAC 2 cut(s) 571, 1341
AccII CGCG 1 cut(s) 351
AciI CCGC 3 cut(s) 8, 297, 349
AclWI GGATC 2 cut(s) 570, 1042
AcoI YGGCCR 1 cut(s) 1146
AcsI RAATTY 1 cut(s) 987
AcuI CTGAAG 3 cut(s) 857, 996, 1263
AfaI GTAC 4 cut(s) 143, 295, 1197, 1211
AfiI CCNNNNNNNGG 6 cut(s) 106, 544, 558, 559, 1124, 1155
AgsI TTSAA 2 cut(s) 698, 931
AhlI ACTAGT 1 cut(s) 148
AjnI CCWGG 1 cut(s) 688
AjuI GAANNNNNNNTTGG 2 cut(s) 874, 906
AluBI AGCT 6 cut(s) 161, 184, 759, 981, 1016, 1251
AluI AGCT 6 cut(s) 161, 184, 759, 981, 1016, 1251
Alw21I GWGCWC 1 cut(s) 717
AlwI GGATC 2 cut(s) 570, 1042
AoxI GGCC 5 cut(s) 229, 341, 662, 686, 1146
ApeKI GCWGC 4 cut(s) 256, 306, 1204, 1297
ApoI RAATTY 1 cut(s) 987
AseI ATTAAT 1 cut(s) 92
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 4 cut(s) 230, 629, 919, 1049
AsuHPI GGTGA 1 cut(s) 358
AsuII TTCGAA 1 cut(s) 1075
AvaII GGWCC 3 cut(s) 629, 919, 1049
BanI GGYRCC 1 cut(s) 1006
BanII GRGCYC 1 cut(s) 552
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 4 cut(s) 243, 293, 1191, 1309
BccI CCATC 4 cut(s) 16, 538, 910, 1235
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 1 cut(s) 690
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 3 cut(s) 149, 753, 816
BfmI CTRYAG 4 cut(s) 337, 790, 1080, 1205
BfoI RGCGCY 1 cut(s) 332
BisI GCNGC 4 cut(s) 257, 307, 1205, 1298
BlpI GCTNAGC 1 cut(s) 810
BlsI GCNGC 4 cut(s) 258, 308, 1206, 1299
Bme1390I CCNGG 1 cut(s) 690
Bme18I GGWCC 3 cut(s) 629, 919, 1049
BmgT120I GGNCC 4 cut(s) 230, 629, 919, 1049
BmiI GGNNCC 3 cut(s) 549, 921, 1008
BmrFI CCNGG 1 cut(s) 690
BmsI GCATC 1 cut(s) 943
BpiI GAAGAC 1 cut(s) 190
BplI GAGNNNNNCTC 2 cut(s) 649, 681
Bpu1102I GCTNAGC 1 cut(s) 810
Bpu14I TTCGAA 1 cut(s) 1075
BpuEI CTTGAG 2 cut(s) 1029, 1273
BsaBI GATNNNNATC 1 cut(s) 648
BsaJI CCNNGG 2 cut(s) 46, 996
BsaWI WCCGGW 1 cut(s) 1188
Bsc4I CCNNNNNNNGG 6 cut(s) 106, 544, 558, 559, 1124, 1155
Bse1I ACTGG 1 cut(s) 121
Bse8I GATNNNNATC 1 cut(s) 648
BseBI CCWGG 1 cut(s) 690
BseDI CCNNGG 2 cut(s) 46, 996
BseGI GGATG 3 cut(s) 782, 855, 921
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 6 cut(s) 106, 544, 558, 559, 1124, 1155
BseMII CTCAG 3 cut(s) 647, 702, 801
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 1 cut(s) 751
BseXI GCAGC 4 cut(s) 243, 293, 1191, 1309
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 351
BshFI GGCC 5 cut(s) 231, 343, 664, 688, 1148
BshNI GGYRCC 1 cut(s) 1006
BsiHKAI GWGCWC 1 cut(s) 717
BsiSI CCGG 2 cut(s) 1173, 1189
BslFI GGGAC 2 cut(s) 137, 905
BslI CCNNNNNNNGG 6 cut(s) 106, 544, 558, 559, 1124, 1155
BsmFI GGGAC 2 cut(s) 137, 905
BsnI GGCC 5 cut(s) 231, 343, 664, 688, 1148
Bsp119I TTCGAA 1 cut(s) 1075
Bsp1286I GDGCHC 2 cut(s) 552, 717
Bsp143I GATC 5 cut(s) 213, 562, 836, 1034, 1177
Bsp1720I GCTNAGC 1 cut(s) 810
BspACI CCGC 3 cut(s) 8, 297, 349
BspANI GGCC 5 cut(s) 231, 343, 664, 688, 1148
BspCNI CTCAG 3 cut(s) 648, 703, 802
BspFNI CGCG 1 cut(s) 351
BspLI GGNNCC 3 cut(s) 549, 921, 1008
BspMAI CTGCAG 1 cut(s) 1209
BspPI GGATC 2 cut(s) 570, 1042
BspQI GCTCTTC 1 cut(s) 1295
BspT104I TTCGAA 1 cut(s) 1075
BspT107I GGYRCC 1 cut(s) 1006
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 2 cut(s) 46, 996
BssMI GATC 5 cut(s) 213, 562, 836, 1034, 1177
BssT1I CCWWGG 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 690
Bst4CI ACNGT 3 cut(s) 719, 794, 1084
Bst6I CTCTTC 2 cut(s) 672, 1295
BstBI TTCGAA 1 cut(s) 1075
BstC8I GCNNGC 3 cut(s) 182, 1041, 1249
BstDEI CTNAG 8 cut(s) 71, 121, 176, 290, 656, 711, 810, 982
BstF5I GGATG 3 cut(s) 782, 855, 921
BstFNI CGCG 1 cut(s) 351
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 5 cut(s) 216, 565, 839, 1037, 1180
BstMBI GATC 5 cut(s) 213, 562, 836, 1034, 1177
BstMWI GCNNNNNNNGC 2 cut(s) 328, 1294
BstNI CCWGG 1 cut(s) 690
BstNSI RCATGY 1 cut(s) 1304
BstSCI CCNGG 1 cut(s) 688
BstSFI CTRYAG 4 cut(s) 337, 790, 1080, 1205
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 4 cut(s) 243, 293, 1191, 1309
BstV2I GAAGAC 1 cut(s) 190
BstXI CCANNNNNNTGG 1 cut(s) 805
BsuRI GGCC 5 cut(s) 231, 343, 664, 688, 1148
BtsCI GGATG 3 cut(s) 782, 855, 921
BtsI GCAGTG 1 cut(s) 1283
BtsIMutI CAGTG 2 cut(s) 715, 1283
Cac8I GCNNGC 3 cut(s) 182, 1041, 1249
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 4 cut(s) 230, 629, 919, 1049
Csp6I GTAC 4 cut(s) 142, 294, 1196, 1210
CviAII CATG 1 cut(s) 1301
CviQI GTAC 4 cut(s) 142, 294, 1196, 1210
DdeI CTNAG 8 cut(s) 71, 121, 176, 290, 656, 711, 810, 982
DpnI GATC 5 cut(s) 215, 564, 838, 1036, 1179
DpnII GATC 5 cut(s) 213, 562, 836, 1034, 1177
EaeI YGGCCR 1 cut(s) 1146
Eam1104I CTCTTC 2 cut(s) 672, 1295
EarI CTCTTC 2 cut(s) 672, 1295
Eco130I CCWWGG 1 cut(s) 46
Eco147I AGGCCT 2 cut(s) 343, 664
Eco24I GRGCYC 1 cut(s) 552
Eco47I GGWCC 3 cut(s) 629, 919, 1049
Eco57I CTGAAG 3 cut(s) 857, 996, 1263
EcoRII CCWGG 1 cut(s) 688
EcoT14I CCWWGG 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 1 cut(s) 1304
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 713, 745
FaqI GGGAC 2 cut(s) 137, 905
FatI CATG 1 cut(s) 1300
FauI CCCGC 1 cut(s) 356
FauNDI CATATG 2 cut(s) 484, 540
FblI GTMKAC 2 cut(s) 571, 1341
Fnu4HI GCNGC 4 cut(s) 257, 307, 1205, 1298
FokI GGATG 3 cut(s) 769, 862, 928
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 4 cut(s) 257, 307, 1205, 1298
FspBI CTAG 3 cut(s) 149, 753, 816
GlaI GCGC 1 cut(s) 330
GluI GCNGC 4 cut(s) 257, 307, 1205, 1298
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 5 cut(s) 231, 343, 664, 688, 1148
HapII CCGG 2 cut(s) 1173, 1189
HhaI GCGC 1 cut(s) 331
Hin1II CATG 1 cut(s) 1304
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 1249
HinfI GANTC 2 cut(s) 363, 1058
HpaII CCGG 2 cut(s) 1173, 1189
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 6 cut(s) 523, 535, 572, 943, 1212, 1342
Hpy188I TCNGA 6 cut(s) 114, 460, 657, 836, 997, 1070
Hpy188III TCNNGA 1 cut(s) 777
Hpy8I GTNNAC 6 cut(s) 523, 535, 572, 943, 1212, 1342
HpyAV CCTTC 1 cut(s) 1020
HpyCH4III ACNGT 3 cut(s) 719, 794, 1084
HpyCH4IV ACGT 1 cut(s) 926
HpyCH4V TGCA 8 cut(s) 77, 413, 737, 956, 1100, 1207, 1247, 1265
HpyF10VI GCNNNNNNNGC 2 cut(s) 328, 1294
HpyF3I CTNAG 8 cut(s) 71, 121, 176, 290, 656, 711, 810, 982
HpySE526I ACGT 1 cut(s) 926
Hsp92II CATG 1 cut(s) 1304
HspAI GCGC 1 cut(s) 329
Kzo9I GATC 5 cut(s) 213, 562, 836, 1034, 1177
LguI GCTCTTC 1 cut(s) 1295
LmnI GCTCC 3 cut(s) 547, 764, 1238
Lsp1109I GCAGC 4 cut(s) 243, 293, 1191, 1309
LweI GCATC 1 cut(s) 943
MaeI CTAG 3 cut(s) 149, 753, 816
MaeII ACGT 1 cut(s) 926
MaeIII GTNAC 3 cut(s) 3, 418, 464
MalI GATC 5 cut(s) 215, 564, 838, 1036, 1179
MboI GATC 5 cut(s) 213, 562, 836, 1034, 1177
MfeI CAATTG 4 cut(s) 414, 885, 936, 1242
MhlI GDGCHC 2 cut(s) 552, 717
MmeI TCCRAC 1 cut(s) 810
MseI TTAA 3 cut(s) 92, 557, 1269
MslI CAYNNNNRTG 2 cut(s) 374, 914
MspI CCGG 2 cut(s) 1173, 1189
MspR9I CCNGG 1 cut(s) 690
MunI CAATTG 4 cut(s) 414, 885, 936, 1242
MvaI CCWGG 1 cut(s) 690
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 328, 1294
NdeI CATATG 2 cut(s) 484, 540
NdeII GATC 5 cut(s) 213, 562, 836, 1034, 1177
NlaIII CATG 1 cut(s) 1304
NlaIV GGNNCC 3 cut(s) 549, 921, 1008
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 1304
NspV TTCGAA 1 cut(s) 1075
PceI AGGCCT 2 cut(s) 343, 664
PciSI GCTCTTC 1 cut(s) 1295
PfeI GAWTC 2 cut(s) 363, 1058
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 4 cut(s) 258, 308, 1206, 1299
PshBI ATTAAT 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 688
PspGI CCWGG 1 cut(s) 688
PspN4I GGNNCC 3 cut(s) 549, 921, 1008
PspPI GGNCC 4 cut(s) 230, 629, 919, 1049
PsrI GAACNNNNNNTAC 2 cut(s) 270, 302
PstI CTGCAG 1 cut(s) 1209
RsaI GTAC 4 cut(s) 143, 295, 1197, 1211
RsaNI GTAC 4 cut(s) 142, 294, 1196, 1210
RseI CAYNNNNRTG 2 cut(s) 374, 914
SapI GCTCTTC 1 cut(s) 1295
SaqAI TTAA 3 cut(s) 92, 557, 1269
SatI GCNGC 4 cut(s) 257, 307, 1205, 1298
Sau3AI GATC 5 cut(s) 213, 562, 836, 1034, 1177
Sau96I GGNCC 4 cut(s) 230, 629, 919, 1049
ScrFI CCNGG 1 cut(s) 690
SduI GDGCHC 2 cut(s) 552, 717
SetI ASST 9 cut(s) 163, 186, 400, 425, 761, 929, 983, 1018, 1253
SfaNI GCATC 1 cut(s) 943
SfcI CTRYAG 4 cut(s) 337, 790, 1080, 1205
SfuI TTCGAA 1 cut(s) 1075
SinI GGWCC 3 cut(s) 629, 919, 1049
SmiMI CAYNNNNRTG 2 cut(s) 374, 914
SmlI CTYRAG 2 cut(s) 1044, 1252
SmoI CTYRAG 2 cut(s) 1044, 1252
SpeI ACTAGT 1 cut(s) 148
SseBI AGGCCT 2 cut(s) 343, 664
SsiI CCGC 3 cut(s) 8, 297, 349
SspI AATATT 1 cut(s) 1162
SspMI CTAG 3 cut(s) 149, 753, 816
StuI AGGCCT 2 cut(s) 343, 664
StyD4I CCNGG 1 cut(s) 688
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 3 cut(s) 719, 794, 1084
TaiI ACGT 1 cut(s) 929
TaqI TCGA 3 cut(s) 361, 769, 1075
TatI WGTACW 1 cut(s) 1209
TfiI GAWTC 2 cut(s) 363, 1058
Tru1I TTAA 3 cut(s) 92, 557, 1269
Tru9I TTAA 3 cut(s) 92, 557, 1269
TscAI CASTG 2 cut(s) 722, 1290
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 256, 306, 1204, 1297
Tsp45I GTSAC 1 cut(s) 3
TspRI CASTG 2 cut(s) 722, 1290
Van91I CCANNNNNTGG 1 cut(s) 544
VpaK11BI GGWCC 3 cut(s) 629, 919, 1049
VspI ATTAAT 1 cut(s) 92
XapI RAATTY 1 cut(s) 987
XceI RCATGY 1 cut(s) 1304
XmiI GTMKAC 2 cut(s) 571, 1341
XspI CTAG 3 cut(s) 149, 753, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.