pycom01g02800

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006630
Physical Location & Seq
Forward (+)
2287676 .. 2288704
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGAGAGGTGGTGTATTTATTCATACTTGTCCAAGCATAAACCATTTCATCCAATTGCAGAATGATGAAGAAAGGCACAACGACCCTAACTTTCATACAAACTTTCCGGATAAGCTTCTTCTGAATATACTCACCAAGGTTGTTTCCTGCTCCTTTCGTTCCCTATATTCAACAAAAATGGTGTGCAAGAACAGCATTCAAAGATTTGAAAAGGTCGACCCGCTAACTTCATGGAGGAGACATGAAGAAGTGTATAAGTTCTTGGAACGATGCATTGAGTGCAACAATCCCGAGTATTTTGAACTCGAAATTGAGTCTTTGAAGATGATGATCTCCAAGGGTCATCAAGCATCAACGTACGTCTATGATGCAATCTTGGTGTGCGAGGGTGGGGATGAAAAAGAGAAAGGCCTTAACCTTCTAGAAAGAGCTCAGCAATTTTTTTGGCCAATTAGGGTAGACAAGGAAGTTAGAGGTAGAATAATAAAACTTCACGAGGACGGTGCTACCGTCAAAGCCTGCAGTGACTGTGAAAGTGCACAAGGTCATTTCACAGTTCGACCCCATTGGGATTTCTGGTGCCATGGCAAATTCAGTAGCTGCAATTCGTATTTGTTTTTTTTTCCTTTACTTTTCTTCAAATTTAGCAAGGTTCTAAAAGATGGCCTAGCACCTAGGCGGCTAGCTATTATATTTCGTGTAAATAAGTGTCTGTTTACATTTAAAATATACATAATTTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

249

Amino Acids

29.16

Weight (kDa)

8.59

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 72 - 146 1.6e-09 Plant tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 579
AccI GTMKAC 2 cut(s) 216, 459
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 2 cut(s) 221, 679
AcoI YGGCCR 1 cut(s) 446
AcsI RAATTY 2 cut(s) 590, 641
AfaI GTAC 1 cut(s) 359
AgsI TTSAA 6 cut(s) 171, 200, 209, 302, 322, 640
AluBI AGCT 4 cut(s) 115, 431, 600, 686
AluI AGCT 4 cut(s) 115, 431, 600, 686
Alw21I GWGCWC 2 cut(s) 433, 541
Alw26I GTCTC 1 cut(s) 232
Alw44I GTGCAC 1 cut(s) 537
AlwNI CAGNNNCTG 2 cut(s) 528, 600
Ama87I CYCGRG 1 cut(s) 290
Aor13HI TCCGGA 1 cut(s) 106
AoxI GGCC 3 cut(s) 409, 446, 664
ApaLI GTGCAC 1 cut(s) 537
ApeKI GCWGC 1 cut(s) 600
ApoI RAATTY 2 cut(s) 590, 641
AspA2I CCTAGG 1 cut(s) 674
AsuHPI GGTGA 1 cut(s) 124
AsuNHI GCTAGC 1 cut(s) 682
AvaI CYCGRG 1 cut(s) 290
AvrII CCTAGG 1 cut(s) 674
BaeGI GKGCMC 1 cut(s) 541
BalI TGGCCA 1 cut(s) 448
BanI GGYRCC 1 cut(s) 579
BanII GRGCYC 1 cut(s) 433
BauI CACGAG 1 cut(s) 494
Bbv12I GWGCWC 2 cut(s) 433, 541
BbvI GCAGC 1 cut(s) 587
BccI CCATC 1 cut(s) 656
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 4 cut(s) 422, 668, 675, 683
BfmI CTRYAG 1 cut(s) 520
BisI GCNGC 2 cut(s) 601, 680
BlnI CCTAGG 1 cut(s) 674
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 2 cut(s) 602, 681
BmeT110I CYCGRG 1 cut(s) 290
BmiI GGNNCC 1 cut(s) 581
BmsI GCATC 3 cut(s) 260, 358, 359
BmtI GCTAGC 1 cut(s) 686
Bpu1102I GCTNAGC 1 cut(s) 432
BsaBI GATNNNNATC 1 cut(s) 329
BsaJI CCNNGG 4 cut(s) 135, 336, 583, 674
BsaWI WCCGGW 1 cut(s) 106
Bse8I GATNNNNATC 1 cut(s) 329
BseAI TCCGGA 1 cut(s) 106
BseDI CCNNGG 4 cut(s) 135, 336, 583, 674
BseGI GGATG 2 cut(s) 48, 400
BseJI GATNNNNATC 1 cut(s) 329
BseMII CTCAG 1 cut(s) 446
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 1 cut(s) 587
BshFI GGCC 3 cut(s) 411, 448, 666
BshNI GGYRCC 1 cut(s) 579
BsiHKAI GWGCWC 2 cut(s) 433, 541
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 1 cut(s) 107
BsiWI CGTACG 1 cut(s) 357
BsmAI GTCTC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 3 cut(s) 411, 448, 666
BsoBI CYCGRG 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 433, 541
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 1 cut(s) 330
Bsp1720I GCTNAGC 1 cut(s) 432
Bsp19I CCATGG 1 cut(s) 583
BspACI CCGC 2 cut(s) 221, 679
BspANI GGCC 3 cut(s) 411, 448, 666
BspCNI CTCAG 1 cut(s) 445
BspEI TCCGGA 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 581
BspMAI CTGCAG 1 cut(s) 524
BspOI GCTAGC 1 cut(s) 686
BspT107I GGYRCC 1 cut(s) 579
BssECI CCNNGG 4 cut(s) 135, 336, 583, 674
BssMI GATC 1 cut(s) 330
BssSI CACGAG 1 cut(s) 494
BssT1I CCWWGG 4 cut(s) 135, 336, 583, 674
Bst2BI CACGAG 1 cut(s) 494
Bst4CI ACNGT 4 cut(s) 503, 511, 530, 556
BstAPI GCANNNNNTGC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 520, 684
BstDEI CTNAG 1 cut(s) 432
BstDSI CCRYGG 1 cut(s) 583
BstF5I GGATG 2 cut(s) 48, 400
BstKTI GATC 1 cut(s) 333
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 1 cut(s) 330
BstMWI GCNNNNNNNGC 2 cut(s) 192, 279
BstSFI CTRYAG 1 cut(s) 520
BstSLI GKGCMC 1 cut(s) 541
BstV1I GCAGC 1 cut(s) 587
BsuRI GGCC 3 cut(s) 411, 448, 666
BtgI CCRYGG 1 cut(s) 583
BtsCI GGATG 2 cut(s) 48, 400
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 529
Cac8I GCNNGC 2 cut(s) 520, 684
CaiI CAGNNNCTG 2 cut(s) 528, 600
Csp6I GTAC 1 cut(s) 358
CviAII CATG 3 cut(s) 231, 242, 584
CviJI RGCY 9 cut(s) 115, 411, 431, 448, 518, 600, 666, 682, 686
CviKI_1 RGCY 9 cut(s) 115, 411, 431, 448, 518, 600, 666, 682, 686
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 1 cut(s) 432
DpnI GATC 1 cut(s) 332
DpnII GATC 1 cut(s) 330
DraI TTTAAA 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 446
Ecl136II GAGCTC 1 cut(s) 431
Eco130I CCWWGG 4 cut(s) 135, 336, 583, 674
Eco147I AGGCCT 1 cut(s) 411
Eco24I GRGCYC 1 cut(s) 433
Eco53kI GAGCTC 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 431
EcoT14I CCWWGG 4 cut(s) 135, 336, 583, 674
EcoT22I ATGCAT 1 cut(s) 275
EcoT38I GRGCYC 1 cut(s) 433
ErhI CCWWGG 4 cut(s) 135, 336, 583, 674
FaeI CATG 3 cut(s) 234, 245, 587
FatI CATG 3 cut(s) 230, 241, 583
FauI CCCGC 1 cut(s) 228
FblI GTMKAC 2 cut(s) 216, 459
Fnu4HI GCNGC 2 cut(s) 601, 680
FokI GGATG 2 cut(s) 35, 407
FriOI GRGCYC 1 cut(s) 433
Fsp4HI GCNGC 2 cut(s) 601, 680
FspBI CTAG 4 cut(s) 422, 668, 675, 683
GluI GCNGC 2 cut(s) 601, 680
HaeIII GGCC 3 cut(s) 411, 448, 666
HapII CCGG 1 cut(s) 107
Hin1II CATG 3 cut(s) 234, 245, 587
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 1 cut(s) 314
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 4 cut(s) 217, 460, 539, 717
Hpy188I TCNGA 1 cut(s) 123
Hpy188III TCNNGA 4 cut(s) 107, 290, 422, 494
Hpy8I GTNNAC 4 cut(s) 217, 460, 539, 717
HpyAV CCTTC 1 cut(s) 428
HpyCH4III ACNGT 4 cut(s) 503, 511, 530, 556
HpyCH4IV ACGT 2 cut(s) 356, 360
HpyCH4V TGCA 8 cut(s) 58, 186, 273, 282, 371, 522, 539, 603
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 279
HpyF3I CTNAG 1 cut(s) 432
HpySE526I ACGT 2 cut(s) 356, 360
Hsp92II CATG 3 cut(s) 234, 245, 587
Kpn2I TCCGGA 1 cut(s) 106
Kzo9I GATC 1 cut(s) 330
LmnI GCTCC 1 cut(s) 155
LpnPI CCDG 4 cut(s) 120, 160, 532, 562
Lsp1109I GCAGC 1 cut(s) 587
LweI GCATC 3 cut(s) 260, 358, 359
MaeI CTAG 4 cut(s) 422, 668, 675, 683
MaeII ACGT 2 cut(s) 356, 360
MaeIII GTNAC 1 cut(s) 524
MalI GATC 1 cut(s) 332
MboI GATC 1 cut(s) 330
MboII GAAGA 5 cut(s) 80, 110, 257, 334, 628
MfeI CAATTG 1 cut(s) 53
MhlI GDGCHC 2 cut(s) 433, 541
MlsI TGGCCA 1 cut(s) 448
MluCI AATT 8 cut(s) 53, 309, 437, 450, 590, 604, 641, 735
MluNI TGGCCA 1 cut(s) 448
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 4 cut(s) 228, 379, 467, 490
Mox20I TGGCCA 1 cut(s) 448
Mph1103I ATGCAT 1 cut(s) 275
MroI TCCGGA 1 cut(s) 106
MscI TGGCCA 1 cut(s) 448
MseI TTAA 2 cut(s) 414, 723
Msp20I TGGCCA 1 cut(s) 448
MspI CCGG 1 cut(s) 107
MunI CAATTG 1 cut(s) 53
Mva1269I GAATGC 1 cut(s) 195
MwoI GCNNNNNNNGC 2 cut(s) 192, 279
NcoI CCATGG 1 cut(s) 583
NdeII GATC 1 cut(s) 330
NheI GCTAGC 1 cut(s) 682
NlaIII CATG 3 cut(s) 234, 245, 587
NlaIV GGNNCC 1 cut(s) 581
NmuCI GTSAC 1 cut(s) 524
NsiI ATGCAT 1 cut(s) 275
PceI AGGCCT 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PctI GAATGC 1 cut(s) 195
Pfl23II CGTACG 1 cut(s) 357
PkrI GCNGC 2 cut(s) 602, 681
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
Psp124BI GAGCTC 1 cut(s) 433
PspLI CGTACG 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 581
PstI CTGCAG 1 cut(s) 524
PstNI CAGNNNCTG 2 cut(s) 528, 600
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SacI GAGCTC 1 cut(s) 433
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 2 cut(s) 414, 723
SatI GCNGC 2 cut(s) 601, 680
Sau3AI GATC 1 cut(s) 330
SchI GAGTC 1 cut(s) 323
SduI GDGCHC 2 cut(s) 433, 541
SfaNI GCATC 3 cut(s) 260, 358, 359
SfcI CTRYAG 1 cut(s) 520
Sse9I AATT 8 cut(s) 53, 309, 437, 450, 590, 604, 641, 735
SseBI AGGCCT 1 cut(s) 411
SsiI CCGC 2 cut(s) 221, 679
SspMI CTAG 4 cut(s) 422, 668, 675, 683
SstI GAGCTC 1 cut(s) 433
StuI AGGCCT 1 cut(s) 411
StyI CCWWGG 4 cut(s) 135, 336, 583, 674
TaaI ACNGT 4 cut(s) 503, 511, 530, 556
TaiI ACGT 2 cut(s) 359, 363
TaqI TCGA 3 cut(s) 216, 306, 559
TasI AATT 8 cut(s) 53, 309, 437, 450, 590, 604, 641, 735
TauI GCSGC 1 cut(s) 682
Tru1I TTAA 2 cut(s) 414, 723
Tru9I TTAA 2 cut(s) 414, 723
TscAI CASTG 1 cut(s) 529
TseFI GTSAC 1 cut(s) 524
TseI GCWGC 1 cut(s) 600
Tsp45I GTSAC 1 cut(s) 524
TspDTI ATGAA 8 cut(s) 11, 37, 81, 83, 219, 258, 411, 729
TspRI CASTG 1 cut(s) 529
VneI GTGCAC 1 cut(s) 537
XapI RAATTY 2 cut(s) 590, 641
XbaI TCTAGA 1 cut(s) 421
XmaJI CCTAGG 1 cut(s) 674
XmiI GTMKAC 2 cut(s) 216, 459
XspI CTAG 4 cut(s) 422, 668, 675, 683
Zsp2I ATGCAT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.