pycom01g15080

Zinc finger protein GIS2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002926
Physical Location & Seq
Reverse (-)
15078364 .. 15080069
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGAGTGTGAATAGCAGTCAAAGCCCAGAACTAGCCAAAAGATACCACAGCAGAGAACGCACATCTTACCGTGATGCTCCATATCCTAGTGAACGCCGCAGTTATAGGCAAGATTGTCTTTGCAATAAATGTAAACGGCCAGGGCATTTTGCAAGGGATTGCCCGAATATGACTGTCTGCAACAACTGTGGACTTCCAGGCCACATTGCAGCAGAATGCAACTCAACAACTATGTGTTGGAACTGCAAGGAGCCTGGACATCTTGCAAACCAATGTTCTAATAATCCAGTCTGCCATATATGTAGTAAGAAAGGCCACCTGGCTCGCGATTGCTCGAACCCTAGTCCTTCATCCCACGATGCAAGGCTCTGCAACAACTGCTACAAACCGGGTCATATTGCTGTCAATTGCACAAACGAGAAGGCTTGCAATAACTGTCGGAAACCTGGACACCTTGCTCGTGACTGCCCCAACGAACCTGTTTGCCACACCTGCAACATATCAGGTCACGTGGCCCGCCATTGCGCCAAGTCAAGCCTAGCCCCAGACATCAGAGGCCCTTTCCGCGACATCACGTGCCGCAACTGCGGGCTGCCAGGCCATATCAGCCGAGAGTGCATATCGATTGTCATCTGCAACAATTGTGGTGGAATGGGTCACCAAGCTTATGAATGCCCTTCTGCGTTGATGATGTACGGTCGCGGCCTACGTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC zf-CCHC_3 zf-CCHC_4

Protein Analysis

239

Amino Acids

26.31

Weight (kDa)

8.87

Isoelectric Point (pI)

69.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 142 - 158 2.2e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 500
Acc36I ACCTGC 1 cut(s) 500
AccII CGCG 3 cut(s) 327, 567, 702
AciI CCGC 6 cut(s) 97, 517, 565, 580, 588, 702
AcoI YGGCCR 1 cut(s) 137
AcvI CACGTG 2 cut(s) 511, 576
AfaI GTAC 1 cut(s) 695
AjnI CCWGG 6 cut(s) 139, 196, 253, 318, 445, 595
AluBI AGCT 1 cut(s) 665
AluI AGCT 1 cut(s) 665
AoxI GGCC 7 cut(s) 137, 199, 313, 513, 556, 598, 703
ApeKI GCWGC 2 cut(s) 209, 592
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 2 cut(s) 514, 557
AsuC2I CCSGG 1 cut(s) 390
AsuHPI GGTGA 1 cut(s) 650
BauI CACGAG 1 cut(s) 459
BbrPI CACGTG 2 cut(s) 511, 576
BbvI GCAGC 2 cut(s) 221, 579
BceAI ACGGC 1 cut(s) 152
BciT130I CCWGG 6 cut(s) 141, 198, 255, 320, 447, 597
BcnI CCSGG 1 cut(s) 390
BfaI CTAG 4 cut(s) 32, 87, 342, 539
BfuAI ACCTGC 1 cut(s) 500
BisI GCNGC 5 cut(s) 97, 210, 580, 593, 703
BlsI GCNGC 5 cut(s) 98, 211, 581, 594, 704
Bme1390I CCNGG 7 cut(s) 141, 198, 255, 320, 390, 447, 597
BmgT120I GGNCC 2 cut(s) 514, 557
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 7 cut(s) 141, 198, 255, 320, 390, 447, 597
BmsI GCATC 2 cut(s) 64, 349
BpuMI CCSGG 1 cut(s) 390
Bsa29I ATCGAT 1 cut(s) 623
BsaAI YACGTR 3 cut(s) 511, 576, 710
BsaBI GATNNNNATC 1 cut(s) 629
BsaJI CCNNGG 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 287
Bse3DI GCAATG 2 cut(s) 204, 520
Bse8I GATNNNNATC 1 cut(s) 629
BseBI CCWGG 6 cut(s) 141, 198, 255, 320, 447, 597
BseCI ATCGAT 1 cut(s) 623
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 1 cut(s) 350
BseJI GATNNNNATC 1 cut(s) 629
BseMI GCAATG 2 cut(s) 204, 520
BseNI ACTGG 1 cut(s) 287
BseXI GCAGC 2 cut(s) 221, 579
Bsh1236I CGCG 3 cut(s) 327, 567, 702
Bsh1285I CGRYCG 1 cut(s) 700
BshFI GGCC 7 cut(s) 139, 201, 315, 515, 558, 600, 705
BshVI ATCGAT 1 cut(s) 623
BsiEI CGRYCG 1 cut(s) 700
BsiSI CCGG 1 cut(s) 389
BsmI GAATGC 2 cut(s) 221, 677
BsnI GGCC 7 cut(s) 139, 201, 315, 515, 558, 600, 705
Bsp68I TCGCGA 1 cut(s) 327
BspACI CCGC 6 cut(s) 97, 517, 565, 580, 588, 702
BspANI GGCC 7 cut(s) 139, 201, 315, 515, 558, 600, 705
BspDI ATCGAT 1 cut(s) 623
BspFNI CGCG 3 cut(s) 327, 567, 702
BspLI GGNNCC 1 cut(s) 252
BspMI ACCTGC 1 cut(s) 500
BsrDI GCAATG 2 cut(s) 204, 520
BsrI ACTGG 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 140
BssSI CACGAG 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 459
Bst2UI CCWGG 6 cut(s) 141, 198, 255, 320, 447, 597
Bst4CI ACNGT 5 cut(s) 71, 175, 188, 437, 698
BstAPI GCANNNNNTGC 1 cut(s) 378
BstBAI YACGTR 3 cut(s) 511, 576, 710
BstC8I GCNNGC 4 cut(s) 325, 427, 517, 590
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 1 cut(s) 350
BstFNI CGCG 3 cut(s) 327, 567, 702
BstHHI GCGC 1 cut(s) 527
BstMCI CGRYCG 1 cut(s) 700
BstMWI GCNNNNNNNGC 8 cut(s) 21, 57, 378, 492, 564, 585, 606, 615
BstNI CCWGG 6 cut(s) 141, 198, 255, 320, 447, 597
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 7 cut(s) 139, 196, 253, 318, 388, 445, 595
BstSNI TACGTA 1 cut(s) 710
BstUI CGCG 3 cut(s) 327, 567, 702
BstV1I GCAGC 2 cut(s) 221, 579
Bsu15I ATCGAT 1 cut(s) 623
BsuRI GGCC 7 cut(s) 139, 201, 315, 515, 558, 600, 705
BsuTUI ATCGAT 1 cut(s) 623
BtsCI GGATG 1 cut(s) 350
BtuMI TCGCGA 1 cut(s) 327
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 4 cut(s) 325, 427, 517, 590
CfoI GCGC 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 514, 557
ClaI ATCGAT 1 cut(s) 623
Csp6I GTAC 1 cut(s) 694
CspCI CAANNNNNGTGG 6 cut(s) 169, 204, 625, 628, 660, 663
CviQI GTAC 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 137
Eco105I TACGTA 1 cut(s) 710
Eco72I CACGTG 2 cut(s) 511, 576
Eco91I GGTNACC 1 cut(s) 656
EcoO109I RGGNCCY 1 cut(s) 557
EcoO65I GGTNACC 1 cut(s) 656
EcoRII CCWGG 6 cut(s) 139, 196, 253, 318, 445, 595
FalI AAGNNNNNCTT 2 cut(s) 102, 134
FauI CCCGC 2 cut(s) 524, 581
Fnu4HI GCNGC 5 cut(s) 97, 210, 580, 593, 703
FokI GGATG 1 cut(s) 337
Fsp4HI GCNGC 5 cut(s) 97, 210, 580, 593, 703
FspBI CTAG 4 cut(s) 32, 87, 342, 539
GlaI GCGC 1 cut(s) 526
GluI GCNGC 5 cut(s) 97, 210, 580, 593, 703
HaeIII GGCC 7 cut(s) 139, 201, 315, 515, 558, 600, 705
HapII CCGG 1 cut(s) 389
HhaI GCGC 1 cut(s) 527
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HindIII AAGCTT 1 cut(s) 663
HpaII CCGG 1 cut(s) 389
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 3 cut(s) 92, 134, 191
Hpy188I TCNGA 2 cut(s) 441, 554
Hpy188III TCNNGA 2 cut(s) 326, 461
Hpy8I GTNNAC 3 cut(s) 92, 134, 191
HpyAV CCTTC 3 cut(s) 357, 415, 687
HpyCH4III ACNGT 5 cut(s) 71, 175, 188, 437, 698
HpyCH4IV ACGT 3 cut(s) 510, 575, 709
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 57, 378, 492, 564, 585, 606, 615
HpySE526I ACGT 3 cut(s) 510, 575, 709
HspAI GCGC 1 cut(s) 525
LmnI GCTCC 2 cut(s) 82, 250
Lsp1109I GCAGC 2 cut(s) 221, 579
LweI GCATC 2 cut(s) 64, 349
MaeI CTAG 4 cut(s) 32, 87, 342, 539
MaeII ACGT 3 cut(s) 510, 575, 709
MaeIII GTNAC 3 cut(s) 461, 506, 656
MfeI CAATTG 2 cut(s) 406, 640
MluCI AATT 2 cut(s) 406, 640
MmeI TCCRAC 2 cut(s) 218, 419
MnlI CCTC 1 cut(s) 548
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 7 cut(s) 141, 198, 255, 320, 390, 447, 597
MunI CAATTG 2 cut(s) 406, 640
Mva1269I GAATGC 2 cut(s) 221, 677
MvaI CCWGG 6 cut(s) 141, 198, 255, 320, 447, 597
MvnI CGCG 3 cut(s) 327, 567, 702
MwoI GCNNNNNNNGC 8 cut(s) 21, 57, 378, 492, 564, 585, 606, 615
NciI CCSGG 1 cut(s) 390
NlaIV GGNNCC 1 cut(s) 252
NmeAIII GCCGAG 1 cut(s) 635
NmuCI GTSAC 3 cut(s) 461, 506, 656
NruI TCGCGA 1 cut(s) 327
PaqCI CACCTGC 1 cut(s) 500
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PctI GAATGC 2 cut(s) 221, 677
PkrI GCNGC 5 cut(s) 98, 211, 581, 594, 704
PmaCI CACGTG 2 cut(s) 511, 576
PmlI CACGTG 2 cut(s) 511, 576
Ppu21I YACGTR 3 cut(s) 511, 576, 710
Psp6I CCWGG 6 cut(s) 139, 196, 253, 318, 445, 595
PspCI CACGTG 2 cut(s) 511, 576
PspEI GGTNACC 1 cut(s) 656
PspGI CCWGG 6 cut(s) 139, 196, 253, 318, 445, 595
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 2 cut(s) 514, 557
RruI TCGCGA 1 cut(s) 327
RsaI GTAC 1 cut(s) 695
RsaNI GTAC 1 cut(s) 694
SatI GCNGC 5 cut(s) 97, 210, 580, 593, 703
Sau96I GGNCC 2 cut(s) 514, 557
ScrFI CCNGG 7 cut(s) 141, 198, 255, 320, 390, 447, 597
SfaNI GCATC 2 cut(s) 64, 349
SnaBI TACGTA 1 cut(s) 710
Sse9I AATT 2 cut(s) 406, 640
SsiI CCGC 6 cut(s) 97, 517, 565, 580, 588, 702
SspMI CTAG 4 cut(s) 32, 87, 342, 539
StyD4I CCNGG 7 cut(s) 139, 196, 253, 318, 388, 445, 595
TaaI ACNGT 5 cut(s) 71, 175, 188, 437, 698
TaiI ACGT 3 cut(s) 513, 578, 712
TaqI TCGA 2 cut(s) 335, 623
TasI AATT 2 cut(s) 406, 640
TauI GCSGC 3 cut(s) 99, 582, 705
TseFI GTSAC 3 cut(s) 461, 506, 656
TseI GCWGC 2 cut(s) 209, 592
Tsp45I GTSAC 3 cut(s) 461, 506, 656
TspDTI ATGAA 2 cut(s) 339, 684
XspI CTAG 4 cut(s) 32, 87, 342, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.