pycom01g22870

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
Physical Location & Seq
Forward (+)
20322960 .. 20323232
273 bp
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UTR
Exon/CDS
Intron
pycom01g22870.1

Sequence Viewer

Length: 273 bp
ATGGGTGATGAGAATGAGGCCCGGAACTACAGCTACAGCTTAGAGGTTGGAGCGAATGGCAGGAAACTCATATGGGAGGGGACACCACGAAGTGTCCGTGATAGCCACCGGAAAGTCAGGGATAGCCACGATGGTCTCATTATCCAACGAAACATGGCTCTATTCTTCTCCGGAGGCGACAGGAAGGAGCTGAAGCTGAGAGTTACCGGGAGGATATGGAAGGAACAGCAAAATCAAGATTCTGGGGTGTGCATACCAAACCTGTGTAGCTAA

Protein Analysis

91

Amino Acids

10.29

Weight (kDa)

9.14

Isoelectric Point (pI)

22.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sina_TRAF PF03145 1 - 69 9.8e-23 Sina, TRAF-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 170
AcuI CTGAAG 1 cut(s) 212
AdeI CACNNNGTG 1 cut(s) 92
AluBI AGCT 5 cut(s) 33, 39, 190, 196, 270
AluI AGCT 5 cut(s) 33, 39, 190, 196, 270
Alw26I GTCTC 1 cut(s) 140
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 19
AsuC2I CCSGG 2 cut(s) 22, 208
AsuHPI GGTGA 1 cut(s) 17
BccI CCATC 1 cut(s) 125
BcnI CCSGG 2 cut(s) 22, 208
BcoDI GTCTC 1 cut(s) 140
BfmI CTRYAG 2 cut(s) 28, 34
Bme1390I CCNGG 2 cut(s) 22, 208
BmgT120I GGNCC 1 cut(s) 19
BmrFI CCNGG 2 cut(s) 22, 208
BpuMI CCSGG 2 cut(s) 22, 208
BsaI GGTCTC 1 cut(s) 140
BsaWI WCCGGW 2 cut(s) 108, 170
BseAI TCCGGA 1 cut(s) 170
BseMII CTCAG 1 cut(s) 188
BshFI GGCC 1 cut(s) 20
BsiSI CCGG 4 cut(s) 22, 109, 171, 207
BslFI GGGAC 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 94
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 1 cut(s) 140
Bsp13I TCCGGA 1 cut(s) 170
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 189
BspEI TCCGGA 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 140
BstDEI CTNAG 2 cut(s) 40, 197
BstMAI GTCTC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 20, 206
BstSFI CTRYAG 2 cut(s) 28, 34
BsuRI GGCC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 19
CviAII CATG 1 cut(s) 154
CviJI RGCY 9 cut(s) 20, 33, 39, 105, 126, 158, 190, 196, 270
CviKI_1 RGCY 9 cut(s) 20, 33, 39, 105, 126, 158, 190, 196, 270
DdeI CTNAG 2 cut(s) 40, 197
DraIII CACNNNGTG 1 cut(s) 92
Eco31I GGTCTC 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 212
FaeI CATG 1 cut(s) 157
FaiI YATR 5 cut(s) 71, 73, 155, 217, 254
FaqI GGGAC 1 cut(s) 94
FatI CATG 1 cut(s) 153
FauNDI CATATG 1 cut(s) 71
HaeIII GGCC 1 cut(s) 20
HapII CCGG 4 cut(s) 22, 109, 171, 207
Hin1II CATG 1 cut(s) 157
HinfI GANTC 1 cut(s) 239
HpaII CCGG 4 cut(s) 22, 109, 171, 207
HphI GGTGA 1 cut(s) 17
Hpy188III TCNNGA 2 cut(s) 171, 236
HpyAV CCTTC 2 cut(s) 178, 214
HpyCH4V TGCA 1 cut(s) 252
HpyF3I CTNAG 2 cut(s) 40, 197
Hsp92II CATG 1 cut(s) 157
Kpn2I TCCGGA 1 cut(s) 170
LmnI GCTCC 2 cut(s) 50, 187
LpnPI CCDG 8 cut(s) 35, 46, 103, 122, 166, 184, 220, 228
MaeIII GTNAC 1 cut(s) 202
MboII GAAGA 1 cut(s) 157
MmeI TCCRAC 2 cut(s) 28, 169
MnlI CCTC 5 cut(s) 10, 37, 70, 167, 204
MroI TCCGGA 1 cut(s) 170
MspI CCGG 4 cut(s) 22, 109, 171, 207
MspR9I CCNGG 2 cut(s) 22, 208
NciI CCSGG 2 cut(s) 22, 208
NdeI CATATG 1 cut(s) 71
NlaIII CATG 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PfeI GAWTC 1 cut(s) 239
PspPI GGNCC 1 cut(s) 19
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
Sau96I GGNCC 1 cut(s) 19
ScrFI CCNGG 2 cut(s) 22, 208
SetI ASST 7 cut(s) 35, 41, 48, 192, 198, 264, 272
SfcI CTRYAG 2 cut(s) 28, 34
StyD4I CCNGG 2 cut(s) 20, 206
TfiI GAWTC 1 cut(s) 239
TspGWI ACGGA 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.