Rh7CG247700

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0005219
Physical Location & Seq
Reverse (-)
23266176 .. 23267404
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG247700.1

Sequence Viewer

Length: 390 bp
ATGGACTTGGAGAGCATAGAGTGTGTTTCATCCTCAGATGGTCTTGATGAGGATGAGATCCATCAACACCTTCACAACCCACAACATCCTTCTCATCAACACCACCATGATTTTTCTTCTTCCAAGCCCAGAAATAATGTTACTAACACCGCCGGAATTCCGGGGCCCACCGCCATTGCTCCGGCCACCAGCGTTCATGAGTTGCTGGAATGTCCGGTGTGTACGAATTCCATGTACCCACCGATTCATCAGTGTCACAATGGACACACACTGTGTTCTACCTGTAAAACAAGGGTCCACAATCGCTGCCCCACTTGTAGGCAGGAGCTTGGAGATATTAGGTGTTTAGCATTGGAGAAGGTGGCTGAGTCCCTGGAGTTACCCTGCTAG

Protein Analysis

129

Amino Acids

14.24

Weight (kDa)

5.84

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sina_RING PF21362 71 - 106 9.7e-14 E3 ubiquitin-protein ligase sina/sinah, RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 150, 171
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 2 cut(s) 156, 226
AdeI CACNNNGTG 1 cut(s) 273
AfaI GTAC 2 cut(s) 223, 236
AfiI CCNNNNNNNGG 1 cut(s) 318
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 1 cut(s) 328
AluI AGCT 1 cut(s) 328
AlwI GGATC 1 cut(s) 52
AoxI GGCC 2 cut(s) 164, 183
ApaI GGGCCC 1 cut(s) 168
ApeKI GCWGC 1 cut(s) 306
ApoI RAATTY 2 cut(s) 156, 226
AspS9I GGNCC 3 cut(s) 164, 165, 295
AsuC2I CCSGG 1 cut(s) 162
AvaII GGWCC 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 293
BccI CCATC 2 cut(s) 32, 69
BciT130I CCWGG 1 cut(s) 374
BcnI CCSGG 1 cut(s) 162
BfaI CTAG 1 cut(s) 388
BisI GCNGC 1 cut(s) 307
BlsI GCNGC 1 cut(s) 308
Bme1390I CCNGG 2 cut(s) 162, 374
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 3 cut(s) 164, 165, 295
BmiI GGNNCC 3 cut(s) 165, 166, 296
BmrFI CCNGG 2 cut(s) 162, 374
BpuMI CCSGG 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 161, 372
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 161, 372
BseGI GGATG 3 cut(s) 29, 58, 85
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 2 cut(s) 48, 357
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 1 cut(s) 293
BshFI GGCC 2 cut(s) 166, 185
BsiSI CCGG 4 cut(s) 153, 161, 182, 215
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 355
BsnI GGCC 2 cut(s) 166, 185
Bsp120I GGGCCC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 1 cut(s) 57
BspACI CCGC 2 cut(s) 150, 171
BspANI GGCC 2 cut(s) 166, 185
BspCNI CTCAG 2 cut(s) 47, 358
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 3 cut(s) 165, 166, 296
BspPI GGATC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 174
BssECI CCNNGG 2 cut(s) 161, 372
BssMI GATC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 273
BstDEI CTNAG 2 cut(s) 34, 366
BstF5I GGATG 3 cut(s) 29, 58, 85
BstKTI GATC 1 cut(s) 60
BstMBI GATC 1 cut(s) 57
BstNI CCWGG 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 160, 372
BstSLI GKGCMC 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 293
BstX2I RGATCY 1 cut(s) 57
BstYI RGATCY 1 cut(s) 57
BsuRI GGCC 2 cut(s) 166, 185
BtsCI GGATG 3 cut(s) 29, 58, 85
BtsIMutI CAGTG 2 cut(s) 257, 269
CciI TCATGA 1 cut(s) 196
Cfr13I GGNCC 3 cut(s) 164, 165, 295
Csp6I GTAC 2 cut(s) 222, 235
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 3 cut(s) 107, 197, 232
CviJI RGCY 5 cut(s) 127, 166, 185, 328, 365
CviKI_1 RGCY 5 cut(s) 127, 166, 185, 328, 365
CviQI GTAC 2 cut(s) 222, 235
DdeI CTNAG 2 cut(s) 34, 366
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
DraIII CACNNNGTG 1 cut(s) 273
EaeI YGGCCR 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 164
EcoRI GAATTC 2 cut(s) 156, 226
EcoRII CCWGG 1 cut(s) 372
EcoT38I GRGCYC 1 cut(s) 168
FaeI CATG 3 cut(s) 110, 200, 235
FaiI YATR 4 cut(s) 17, 108, 198, 233
FaqI GGGAC 1 cut(s) 355
FatI CATG 3 cut(s) 106, 196, 231
Fnu4HI GCNGC 1 cut(s) 307
FokI GGATG 3 cut(s) 16, 65, 72
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 307
FspBI CTAG 1 cut(s) 388
GluI GCNGC 1 cut(s) 307
HaeIII GGCC 2 cut(s) 166, 185
HapII CCGG 4 cut(s) 153, 161, 182, 215
Hin1II CATG 3 cut(s) 110, 200, 235
HinfI GANTC 2 cut(s) 244, 368
HpaII CCGG 4 cut(s) 153, 161, 182, 215
Hpy166II GTNNAC 2 cut(s) 222, 298
Hpy188I TCNGA 1 cut(s) 37
Hpy188III TCNNGA 2 cut(s) 44, 197
Hpy8I GTNNAC 2 cut(s) 222, 298
HpyAV CCTTC 3 cut(s) 80, 99, 352
HpyCH4III ACNGT 1 cut(s) 273
HpyF3I CTNAG 2 cut(s) 34, 366
Hsp92II CATG 3 cut(s) 110, 200, 235
Kzo9I GATC 1 cut(s) 57
LmnI GCTCC 2 cut(s) 184, 325
Lsp1109I GCAGC 1 cut(s) 293
MaeI CTAG 1 cut(s) 388
MaeIII GTNAC 3 cut(s) 139, 254, 378
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 2 cut(s) 108, 111
MflI RGATCY 1 cut(s) 57
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 2 cut(s) 156, 226
MlyI GAGTC 1 cut(s) 377
MnlI CCTC 2 cut(s) 43, 43
MslI CAYNNNNRTG 1 cut(s) 105
MspI CCGG 4 cut(s) 153, 161, 182, 215
MspR9I CCNGG 2 cut(s) 162, 374
MvaI CCWGG 1 cut(s) 374
NciI CCSGG 1 cut(s) 162
NdeII GATC 1 cut(s) 57
NlaIII CATG 3 cut(s) 110, 200, 235
NlaIV GGNNCC 3 cut(s) 165, 166, 296
NmuCI GTSAC 1 cut(s) 254
PagI TCATGA 1 cut(s) 196
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 1 cut(s) 308
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 3 cut(s) 165, 166, 296
PspOMI GGGCCC 1 cut(s) 164
PspPI GGNCC 3 cut(s) 164, 165, 295
PsuI RGATCY 1 cut(s) 57
RsaI GTAC 2 cut(s) 223, 236
RsaNI GTAC 2 cut(s) 222, 235
RseI CAYNNNNRTG 1 cut(s) 105
SatI GCNGC 1 cut(s) 307
Sau3AI GATC 1 cut(s) 57
Sau96I GGNCC 3 cut(s) 164, 165, 295
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 162, 374
SduI GDGCHC 1 cut(s) 168
SetI ASST 5 cut(s) 72, 284, 330, 344, 363
SinI GGWCC 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 105
Sse9I AATT 2 cut(s) 156, 226
SsiI CCGC 2 cut(s) 150, 171
SspMI CTAG 1 cut(s) 388
StyD4I CCNGG 2 cut(s) 160, 372
TaaI ACNGT 1 cut(s) 273
TasI AATT 2 cut(s) 156, 226
TfiI GAWTC 1 cut(s) 244
TscAI CASTG 2 cut(s) 257, 276
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 1 cut(s) 306
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 3 cut(s) 18, 185, 236
TspRI CASTG 2 cut(s) 257, 276
VpaK11BI GGWCC 1 cut(s) 295
XapI RAATTY 2 cut(s) 156, 226
XspI CTAG 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.