Rw7G019960

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005219
Physical Location & Seq
Reverse (-)
22315127 .. 22318450
3324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G019960.1

Sequence Viewer

Length: 444 bp
ATGCACACAGGATGCACGTTCAACCATCGCTATGTAAAGTCAAATCCCCGGGAAGTAGAGAATGCCACTTGGATGCTTACAGTCTTCCATTGTTTTGGTCAATACTTCTGCCTCCATTTTGAAGCCTTCCAGCTTGGCATGGCTCCTGTTTATATGGCTTTTCTACGTTTTATGGGTGATGAGAATGAGGCACGGAACTACAGCTACAGCCTCGAGGTTGGAGCAAATGGCAGGAAACTCATATGGGAGGGCACCCCAAGAAGTGTCCGTGATAGCCACCGGAAGGTAAGGGATAGCCATGACGGTCTCATTATTCAACGAAACATGGCTTTATTTTTCTCTGGAGGAGATAGGAAGGAGCTTAAGCTGAGAGTTACCGGGAGGATATGGAAGGAACAGCAAAATCCAGATGCTGGAGTGTGCATACCAAACTTATGTAGCTAA

Protein Analysis

147

Amino Acids

17.05

Weight (kDa)

8.9

Isoelectric Point (pI)

27.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sina_TRAF PF03145 29 - 126 2.2e-35 Sina, TRAF-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 413
AfiI CCNNNNNNNGG 2 cut(s) 283, 413
AflII CTTAAG 1 cut(s) 362
AgsI TTSAA 3 cut(s) 22, 122, 317
AluBI AGCT 5 cut(s) 133, 204, 361, 367, 441
AluI AGCT 5 cut(s) 133, 204, 361, 367, 441
Alw26I GTCTC 1 cut(s) 311
AlwNI CAGNNNCTG 1 cut(s) 413
Ama87I CYCGRG 2 cut(s) 48, 212
AsuC2I CCSGG 3 cut(s) 49, 50, 379
AsuHPI GGTGA 1 cut(s) 188
AvaI CYCGRG 2 cut(s) 48, 212
BaeGI GKGCMC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 76
BccI CCATC 1 cut(s) 33
BcnI CCSGG 3 cut(s) 49, 50, 379
BcoDI GTCTC 1 cut(s) 311
BfmI CTRYAG 2 cut(s) 199, 205
BfrI CTTAAG 1 cut(s) 362
Bme1390I CCNGG 3 cut(s) 49, 50, 379
BmeT110I CYCGRG 2 cut(s) 48, 212
BmiI GGNNCC 2 cut(s) 144, 253
BmrFI CCNGG 3 cut(s) 49, 50, 379
BmsI GCATC 3 cut(s) 2, 63, 400
BpiI GAAGAC 1 cut(s) 76
BpmI CTGGAG 2 cut(s) 363, 435
BpuMI CCSGG 3 cut(s) 49, 50, 379
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 47, 48
BsaWI WCCGGW 1 cut(s) 279
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 413
BseDI CCNNGG 2 cut(s) 47, 48
BseGI GGATG 2 cut(s) 17, 78
BseLI CCNNNNNNNGG 2 cut(s) 283, 413
BseMII CTCAG 1 cut(s) 359
BseRI GAGGAG 1 cut(s) 360
BseSI GKGCMC 1 cut(s) 254
BshNI GGYRCC 1 cut(s) 251
BsiHKCI CYCGRG 2 cut(s) 48, 212
BsiSI CCGG 3 cut(s) 49, 280, 378
BslI CCNNNNNNNGG 2 cut(s) 283, 413
BsmAI GTCTC 1 cut(s) 311
BsmI GAATGC 1 cut(s) 67
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 2 cut(s) 48, 212
Bsp1286I GDGCHC 1 cut(s) 254
BspCNI CTCAG 1 cut(s) 360
BspLI GGNNCC 2 cut(s) 144, 253
BspT107I GGYRCC 1 cut(s) 251
BspTI CTTAAG 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 311
BssECI CCNNGG 2 cut(s) 47, 48
Bst4CI ACNGT 2 cut(s) 82, 305
BstAFI CTTAAG 1 cut(s) 362
BstDEI CTNAG 1 cut(s) 368
BstF5I GGATG 2 cut(s) 17, 78
BstMAI GTCTC 1 cut(s) 311
BstSCI CCNGG 3 cut(s) 47, 48, 377
BstSFI CTRYAG 2 cut(s) 199, 205
BstSLI GKGCMC 1 cut(s) 254
BstV2I GAAGAC 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 95
BtgZI GCGATG 1 cut(s) 11
BtsCI GGATG 2 cut(s) 17, 78
CaiI CAGNNNCTG 1 cut(s) 413
Cfr9I CCCGGG 1 cut(s) 48
CviAII CATG 3 cut(s) 139, 299, 325
DdeI CTNAG 1 cut(s) 368
Eco31I GGTCTC 1 cut(s) 311
Eco88I CYCGRG 2 cut(s) 48, 212
FaeI CATG 3 cut(s) 142, 302, 328
FatI CATG 3 cut(s) 138, 298, 324
FauNDI CATATG 1 cut(s) 242
FokI GGATG 2 cut(s) 24, 85
GsuI CTGGAG 2 cut(s) 363, 435
HapII CCGG 3 cut(s) 49, 280, 378
Hin1II CATG 3 cut(s) 142, 302, 328
HpaII CCGG 3 cut(s) 49, 280, 378
HphI GGTGA 1 cut(s) 188
Hpy188III TCNNGA 2 cut(s) 342, 407
HpyAV CCTTC 4 cut(s) 136, 277, 349, 385
HpyCH4III ACNGT 2 cut(s) 82, 305
HpyCH4IV ACGT 2 cut(s) 17, 166
HpyCH4V TGCA 3 cut(s) 4, 15, 423
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 2 cut(s) 17, 166
Hsp92II CATG 3 cut(s) 142, 302, 328
LmnI GCTCC 3 cut(s) 148, 221, 358
LpnPI CCDG 9 cut(s) 62, 143, 159, 217, 293, 327, 391, 399, 420
LweI GCATC 3 cut(s) 2, 63, 400
MaeII ACGT 2 cut(s) 17, 166
MaeIII GTNAC 1 cut(s) 373
MboII GAAGA 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 7 cut(s) 122, 181, 208, 221, 241, 338, 375
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 2 cut(s) 30, 71
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 3 cut(s) 49, 280, 378
MspR9I CCNGG 3 cut(s) 49, 50, 379
Mva1269I GAATGC 1 cut(s) 67
NciI CCSGG 3 cut(s) 49, 50, 379
NdeI CATATG 1 cut(s) 242
NlaIII CATG 3 cut(s) 142, 302, 328
NlaIV GGNNCC 2 cut(s) 144, 253
PaeR7I CTCGAG 1 cut(s) 212
PctI GAATGC 1 cut(s) 67
PflMI CCANNNNNTGG 1 cut(s) 413
PspN4I GGNNCC 2 cut(s) 144, 253
PspXI VCTCGAGB 1 cut(s) 212
PsrI GAACNNNNNNTAC 2 cut(s) 188, 220
PstNI CAGNNNCTG 1 cut(s) 413
RseI CAYNNNNRTG 2 cut(s) 30, 71
SaqAI TTAA 1 cut(s) 363
ScrFI CCNGG 3 cut(s) 49, 50, 379
SduI GDGCHC 1 cut(s) 254
SetI ASST 9 cut(s) 20, 135, 169, 206, 219, 288, 363, 369, 443
SfaNI GCATC 3 cut(s) 2, 63, 400
SfcI CTRYAG 2 cut(s) 199, 205
Sfr274I CTCGAG 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 212
SmaI CCCGGG 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 30, 71
SmlI CTYRAG 2 cut(s) 212, 362
SmoI CTYRAG 2 cut(s) 212, 362
StyD4I CCNGG 3 cut(s) 47, 48, 377
TaaI ACNGT 2 cut(s) 82, 305
TaiI ACGT 2 cut(s) 20, 169
TaqI TCGA 1 cut(s) 213
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TspGWI ACGGA 2 cut(s) 208, 257
TspMI CCCGGG 1 cut(s) 48
Van91I CCANNNNNTGG 1 cut(s) 413
Vha464I CTTAAG 1 cut(s) 362
XhoI CTCGAG 1 cut(s) 212
XmaI CCCGGG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.