pycom01g23740
NAC Family

Nascent polypeptide-associated complex subunit alpha-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006523
Physical Location & Seq
Forward (+)
20978626 .. 20980007
1382 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23740.1

Sequence Viewer

Length: 657 bp
ATGTCACCGGCTCCACTGGTTCAGCGGGAAGCCGCCGCAGCCGACGAGGTCTCCTCCGTCGAGCAGCTTCTCAAGAGCTCTAATCCGAACAAGGAGGAAGACGAAGTGGTGGTCGAGGACGTGAAGGACGACAATGACGAGGACGAGGACGATGACGAGGACGACGATGATAAGGAAGACGACGCTCAAGGTGGAAGTGAGGCTTCGAAGCAGAGTAGAAGTGAGAAGAAGAGTCGAAAGGCAATGTTGAAGCTTGGGATGAAGCCTGTTACCGGCGTTAGCAGGGTTACCATCAAAAGGACCAAAAACATACTATTTTTCATCTCGAAACCTGATGTGTTCAAGAGTCCAAACTCTGACGCCTATGTCATATTTGGCGAGGCCAAGATCGAGGATTTGAGCTCCCAGCTTCAGACACAGGCTGCTCAGCAGTTCAGGATGCCAGACATGAGTTCTGTGATGGGGAAACCAGAGATTTCGGCAGCAGCTGCTGGAGCTCAGGATGAAGAGGAGGAAGAGGTTGATGAAACCGGGGTGGAGCCTAGGGACATTGATTTGGTCATGACACAGGCTGGGGTATCGAGGAGCAAGGCCGTCAAGGCGCTCAAGACTCACAGCGGGGACATTGTCAGCGCTATCATGGAGCTGACCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAC

Protein Analysis

219

Amino Acids

23.74

Weight (kDa)

4.38

Isoelectric Point (pI)

56.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAC PF01849 75 - 130 3.7e-21 NAC domain
UBA_HYPK PF19026 178 - 217 1.9e-12 HYPK UBA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
AciI CCGC 4 cut(s) 25, 33, 36, 618
AcuI CTGAAG 1 cut(s) 395
AcyI GRCGYC 1 cut(s) 360
AfeI AGCGCT 1 cut(s) 634
AfiI CCNNNNNNNGG 2 cut(s) 272, 297
AgsI TTSAA 2 cut(s) 250, 343
AjiI CACGTC 1 cut(s) 121
AluBI AGCT 8 cut(s) 67, 78, 253, 402, 409, 488, 497, 646
AluI AGCT 8 cut(s) 67, 78, 253, 402, 409, 488, 497, 646
Alw21I GWGCWC 3 cut(s) 80, 404, 499
Alw26I GTCTC 1 cut(s) 55
AlwNI CAGNNNCTG 2 cut(s) 488, 491
Aor51HI AGCGCT 1 cut(s) 634
AoxI GGCC 2 cut(s) 381, 591
ApeKI GCWGC 6 cut(s) 38, 64, 422, 482, 485, 488
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AspA2I CCTAGG 1 cut(s) 542
AspLEI GCGC 2 cut(s) 604, 635
AspS9I GGNCC 1 cut(s) 300
AsuC2I CCSGG 1 cut(s) 532
AsuII TTCGAA 1 cut(s) 206
AvaII GGWCC 1 cut(s) 300
AvrII CCTAGG 1 cut(s) 542
BanII GRGCYC 3 cut(s) 80, 404, 499
BbsI GAAGAC 2 cut(s) 105, 183
Bbv12I GWGCWC 3 cut(s) 80, 404, 499
BbvI GCAGC 6 cut(s) 50, 76, 409, 475, 494, 497
BccI CCATC 2 cut(s) 299, 454
BceAI ACGGC 1 cut(s) 578
BcnI CCSGG 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 543
BfoI RGCGCY 2 cut(s) 605, 636
BglI GCCNNNNNGGC 1 cut(s) 599
BisI GCNGC 8 cut(s) 33, 36, 39, 65, 423, 483, 486, 489
BlnI CCTAGG 1 cut(s) 542
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 8 cut(s) 34, 37, 40, 66, 424, 484, 487, 490
Bme1390I CCNGG 1 cut(s) 532
Bme18I GGWCC 1 cut(s) 300
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 300
BmiI GGNNCC 2 cut(s) 12, 540
BmrFI CCNGG 1 cut(s) 532
BmsI GCATC 1 cut(s) 429
BpiI GAAGAC 2 cut(s) 105, 183
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpmI CTGGAG 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 498
Bpu1102I GCTNAGC 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 3 cut(s) 56, 171, 590
BpuMI CCSGG 1 cut(s) 532
BsaHI GRCGYC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 2 cut(s) 531, 542
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 297
Bse118I RCCGGY 2 cut(s) 7, 272
Bse1I ACTGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 531, 542
BseGI GGATG 3 cut(s) 264, 444, 508
BseLI CCNNNNNNNGG 2 cut(s) 272, 297
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 2 cut(s) 440, 512
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 3 cut(s) 43, 524, 598
BseXI GCAGC 6 cut(s) 50, 76, 409, 475, 494, 497
BseYI CCCAGC 2 cut(s) 405, 572
BshFI GGCC 2 cut(s) 383, 593
BsiHKAI GWGCWC 3 cut(s) 80, 404, 499
BsiSI CCGG 3 cut(s) 8, 273, 531
BslFI GGGAC 2 cut(s) 560, 635
BslI CCNNNNNNNGG 2 cut(s) 272, 297
BsmAI GTCTC 1 cut(s) 55
BsmFI GGGAC 2 cut(s) 560, 635
BsnI GGCC 2 cut(s) 383, 593
Bso31I GGTCTC 1 cut(s) 55
Bsp119I TTCGAA 1 cut(s) 206
Bsp1286I GDGCHC 3 cut(s) 80, 404, 499
Bsp143I GATC 1 cut(s) 387
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 4 cut(s) 25, 33, 36, 618
BspANI GGCC 2 cut(s) 383, 593
BspCNI CTCAG 2 cut(s) 439, 511
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 12, 540
BspT104I TTCGAA 1 cut(s) 206
BspTNI GGTCTC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 2 cut(s) 7, 272
BsrI ACTGG 1 cut(s) 21
BssAI RCCGGY 2 cut(s) 7, 272
BssECI CCNNGG 2 cut(s) 531, 542
BssMI GATC 1 cut(s) 387
BssNI GRCGYC 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 542
Bst6I CTCTTC 3 cut(s) 224, 501, 510
BstACI GRCGYC 1 cut(s) 360
BstAPI GCANNNNNTGC 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 206
BstDEI CTNAG 2 cut(s) 426, 498
BstEII GGTNACC 1 cut(s) 286
BstF5I GGATG 3 cut(s) 264, 444, 508
BstH2I RGCGCY 2 cut(s) 605, 636
BstHHI GCGC 2 cut(s) 604, 635
BstKTI GATC 1 cut(s) 390
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 1 cut(s) 387
BstMWI GCNNNNNNNGC 4 cut(s) 38, 488, 494, 599
BstPI GGTNACC 1 cut(s) 286
BstSCI CCNGG 1 cut(s) 530
BstV1I GCAGC 6 cut(s) 50, 76, 409, 475, 494, 497
BstV2I GAAGAC 2 cut(s) 105, 183
BsuRI GGCC 2 cut(s) 383, 593
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 3 cut(s) 264, 444, 508
BtsIMutI CAGTG 1 cut(s) 14
CaiI CAGNNNCTG 2 cut(s) 488, 491
CciI TCATGA 1 cut(s) 561
CfoI GCGC 2 cut(s) 604, 635
Cfr10I RCCGGY 2 cut(s) 7, 272
Cfr13I GGNCC 1 cut(s) 300
CseI GACGC 2 cut(s) 191, 368
CviAII CATG 3 cut(s) 448, 562, 640
DdeI CTNAG 2 cut(s) 426, 498
DpnI GATC 1 cut(s) 389
DpnII GATC 1 cut(s) 387
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
Eam1104I CTCTTC 3 cut(s) 224, 501, 510
EarI CTCTTC 3 cut(s) 224, 501, 510
Ecl136II GAGCTC 3 cut(s) 78, 402, 497
Eco130I CCWWGG 1 cut(s) 542
Eco24I GRGCYC 3 cut(s) 80, 404, 499
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 300
Eco47III AGCGCT 1 cut(s) 634
Eco53kI GAGCTC 3 cut(s) 78, 402, 497
Eco57I CTGAAG 1 cut(s) 395
Eco91I GGTNACC 1 cut(s) 286
EcoICRI GAGCTC 3 cut(s) 78, 402, 497
EcoO65I GGTNACC 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 542
EcoT38I GRGCYC 3 cut(s) 80, 404, 499
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 3 cut(s) 451, 565, 643
FaiI YATR 7 cut(s) 311, 366, 371, 449, 563, 641, 655
FalI AAGNNNNNCTT 2 cut(s) 187, 219
FaqI GGGAC 2 cut(s) 560, 635
FatI CATG 3 cut(s) 447, 561, 639
FauI CCCGC 2 cut(s) 18, 611
Fnu4HI GCNGC 8 cut(s) 33, 36, 39, 65, 423, 483, 486, 489
FokI GGATG 3 cut(s) 271, 451, 515
FriOI GRGCYC 3 cut(s) 80, 404, 499
Fsp4HI GCNGC 8 cut(s) 33, 36, 39, 65, 423, 483, 486, 489
FspBI CTAG 1 cut(s) 543
GlaI GCGC 2 cut(s) 603, 634
GluI GCNGC 8 cut(s) 33, 36, 39, 65, 423, 483, 486, 489
GsaI CCCAGC 2 cut(s) 409, 576
GsuI CTGGAG 1 cut(s) 513
HaeII RGCGCY 2 cut(s) 605, 636
HaeIII GGCC 2 cut(s) 383, 593
HapII CCGG 3 cut(s) 8, 273, 531
HgaI GACGC 2 cut(s) 191, 368
HhaI GCGC 2 cut(s) 604, 635
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 3 cut(s) 451, 565, 643
Hin6I GCGC 2 cut(s) 602, 633
HinP1I GCGC 2 cut(s) 602, 633
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 3 cut(s) 232, 346, 610
HpaII CCGG 3 cut(s) 8, 273, 531
Hpy188I TCNGA 3 cut(s) 87, 358, 414
Hpy188III TCNNGA 7 cut(s) 73, 325, 343, 436, 500, 562, 607
Hpy99I CGWCG 4 cut(s) 47, 62, 167, 185
HpyAV CCTTC 1 cut(s) 118
HpyCH4IV ACGT 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 488, 494, 599
HpyF3I CTNAG 2 cut(s) 426, 498
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 3 cut(s) 451, 565, 643
HspAI GCGC 2 cut(s) 602, 633
Kzo9I GATC 1 cut(s) 387
LmnI GCTCC 6 cut(s) 16, 407, 494, 538, 585, 643
Lsp1109I GCAGC 6 cut(s) 50, 76, 409, 475, 494, 497
LweI GCATC 1 cut(s) 429
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 3 cut(s) 3, 268, 286
MalI GATC 1 cut(s) 389
MboI GATC 1 cut(s) 387
MboII GAAGA 6 cut(s) 110, 188, 238, 241, 518, 527
MhlI GDGCHC 3 cut(s) 80, 404, 499
MlyI GAGTC 3 cut(s) 241, 355, 604
MspA1I CMGCKG 3 cut(s) 25, 488, 618
MspI CCGG 3 cut(s) 8, 273, 531
MspR9I CCNGG 1 cut(s) 532
MwoI GCNNNNNNNGC 4 cut(s) 38, 488, 494, 599
NciI CCSGG 1 cut(s) 532
NdeII GATC 1 cut(s) 387
NlaIII CATG 3 cut(s) 451, 565, 643
NlaIV GGNNCC 2 cut(s) 12, 540
NmuCI GTSAC 1 cut(s) 3
NspV TTCGAA 1 cut(s) 206
PagI TCATGA 1 cut(s) 561
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 135, 162
PflFI GACNNNGTC 2 cut(s) 47, 626
PkrI GCNGC 8 cut(s) 34, 37, 40, 66, 424, 484, 487, 490
PleI GAGTC 3 cut(s) 240, 354, 604
PpsI GAGTC 3 cut(s) 240, 354, 604
Psp124BI GAGCTC 3 cut(s) 80, 404, 499
PspEI GGTNACC 1 cut(s) 286
PspFI CCCAGC 2 cut(s) 405, 572
PspN4I GGNNCC 2 cut(s) 12, 540
PspPI GGNCC 1 cut(s) 300
PstNI CAGNNNCTG 2 cut(s) 488, 491
PsyI GACNNNGTC 2 cut(s) 47, 626
PvuII CAGCTG 1 cut(s) 488
SacI GAGCTC 3 cut(s) 80, 404, 499
SatI GCNGC 8 cut(s) 33, 36, 39, 65, 423, 483, 486, 489
Sau3AI GATC 1 cut(s) 387
Sau96I GGNCC 1 cut(s) 300
SchI GAGTC 3 cut(s) 241, 355, 604
ScrFI CCNGG 1 cut(s) 532
SduI GDGCHC 3 cut(s) 80, 404, 499
SfaNI GCATC 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 206
SinI GGWCC 1 cut(s) 300
SmlI CTYRAG 3 cut(s) 71, 186, 605
SmoI CTYRAG 3 cut(s) 71, 186, 605
SsiI CCGC 4 cut(s) 25, 33, 36, 618
SspMI CTAG 1 cut(s) 543
SstI GAGCTC 3 cut(s) 80, 404, 499
StyD4I CCNGG 1 cut(s) 530
StyI CCWWGG 1 cut(s) 542
TaiI ACGT 1 cut(s) 123
TaqI TCGA 7 cut(s) 60, 114, 206, 235, 326, 390, 581
TauI GCSGC 2 cut(s) 35, 38
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 6 cut(s) 38, 64, 422, 482, 485, 488
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 4 cut(s) 275, 310, 519, 540
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 1 cut(s) 21
Tth111I GACNNNGTC 2 cut(s) 47, 626
VpaK11BI GGWCC 1 cut(s) 300
XmaJI CCTAGG 1 cut(s) 542
XspI CTAG 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.