pycom01g23750
NAC Family

Nascent polypeptide-associated complex subunit alpha-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006523
Physical Location & Seq
Forward (+)
20981072 .. 20982294
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23750.4

Sequence Viewer

Length: 681 bp
ATGTCACCGGCTCCACTGGTTCAGCCCGAGGCCGCGGCCGCGGCCGCCGAAGAGGTTGTCTCCGCCGACCAGCTTCTCAAGAACACTAATCAGAACAAGGAAGAAGAAGAAGTCGTGGTCGAGGACGTCAAGGACGACGACAAGGACGAGGACGAGGACGATGAGGACGAGGACGAGGACGACGATGATGATAAGGAAGACGCCGGTCAAGGTGGAAATGAGTCATCGAAACAGAGCAGAAGCGAGAAGAAGAGTCGGAAGGCAATGCTGAAGCTGGGTATGAAGCCTGTTACTGGTATTAGCAGGGTTACAATCAAGAAGACGAAAAACATACTATTTGTCATCTCGAAACCCGATGCCTTCAAGAGTCCAAACTCCGATACCTATGTGATATTTGGCGAGGCCAAGATTGAGGATTTGAGCTCTCAGCTGCAGACACAGGCTGCCCAGCAGTTCAGGATGCCGGACATGAGTTCTGTGATGGGGAAACCAGAGATTTCTGGAGCAGCTGCCGGACCTCAGGCAGATGAAGAAGAGGAAGAGGTTGATGAAACCGGGGTGGAACCTAAGGACATTGATTTGGTCATGACACAGGCCGGGGTGTCAAGGAGCAAGGCTGTCAAGGCTCTCAAGACTCACAGCGGGGACATTGTCAGCGCTATCATGGAGCTCACCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAC

Protein Analysis

227

Amino Acids

24.49

Weight (kDa)

4.31

Isoelectric Point (pI)

55.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 129
AccII CGCG 2 cut(s) 35, 41
AciI CCGC 7 cut(s) 33, 35, 39, 41, 45, 63, 642
AcoI YGGCCR 2 cut(s) 36, 42
AcuI CTGAAG 1 cut(s) 290
AcyI GRCGYC 2 cut(s) 126, 201
AfeI AGCGCT 1 cut(s) 658
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 1 cut(s) 364
AluBI AGCT 6 cut(s) 73, 274, 423, 430, 509, 670
AluI AGCT 6 cut(s) 73, 274, 423, 430, 509, 670
Alw21I GWGCWC 2 cut(s) 425, 672
Alw26I GTCTC 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 26
Aor51HI AGCGCT 1 cut(s) 658
AoxI GGCC 5 cut(s) 30, 36, 42, 402, 594
ApeKI GCWGC 4 cut(s) 430, 443, 506, 509
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 515
AsuC2I CCSGG 2 cut(s) 556, 598
AsuHPI GGTGA 1 cut(s) 664
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 515
AxyI CCTNAGG 2 cut(s) 519, 567
BaeI ACNNNNGTAYC 2 cut(s) 372, 405
BanII GRGCYC 2 cut(s) 425, 672
BbsI GAAGAC 2 cut(s) 204, 326
Bbv12I GWGCWC 2 cut(s) 425, 672
BbvI GCAGC 4 cut(s) 417, 430, 496, 518
BccI CCATC 1 cut(s) 475
BcnI CCSGG 2 cut(s) 556, 598
BcoDI GTCTC 1 cut(s) 64
BfmI CTRYAG 1 cut(s) 431
BfoI RGCGCY 1 cut(s) 660
BisI GCNGC 9 cut(s) 33, 36, 39, 42, 45, 431, 444, 507, 510
BlsI GCNGC 9 cut(s) 34, 37, 40, 43, 46, 432, 445, 508, 511
Bme1390I CCNGG 2 cut(s) 556, 598
Bme18I GGWCC 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 515
BmiI GGNNCC 2 cut(s) 12, 564
BmrFI CCNGG 2 cut(s) 556, 598
BmsI GCATC 2 cut(s) 346, 450
BoxI GACNNNNGTC 1 cut(s) 204
BpiI GAAGAC 2 cut(s) 204, 326
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 1 cut(s) 522
BpuEI CTTGAG 2 cut(s) 62, 614
BpuMI CCSGG 2 cut(s) 556, 598
BsaHI GRCGYC 2 cut(s) 126, 201
BsaJI CCNNGG 5 cut(s) 27, 33, 39, 555, 597
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse118I RCCGGY 2 cut(s) 7, 203
Bse1I ACTGG 2 cut(s) 21, 298
Bse21I CCTNAGG 2 cut(s) 519, 567
Bse3DI GCAATG 1 cut(s) 270
BseDI CCNNGG 5 cut(s) 27, 33, 39, 555, 597
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 2 cut(s) 440, 533
BseNI ACTGG 2 cut(s) 21, 298
BseX3I CGGCCG 2 cut(s) 36, 42
BseXI GCAGC 4 cut(s) 417, 430, 496, 518
BseYI CCCAGC 2 cut(s) 274, 447
Bsh1236I CGCG 2 cut(s) 35, 41
Bsh1285I CGRYCG 2 cut(s) 39, 45
BshFI GGCC 5 cut(s) 32, 38, 44, 404, 596
BsiEI CGRYCG 2 cut(s) 39, 45
BsiHKAI GWGCWC 2 cut(s) 425, 672
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 6 cut(s) 8, 204, 464, 513, 555, 597
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 5 cut(s) 32, 38, 44, 404, 596
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 425, 672
BspACI CCGC 7 cut(s) 33, 35, 39, 41, 45, 63, 642
BspANI GGCC 5 cut(s) 32, 38, 44, 404, 596
BspCNI CTCAG 2 cut(s) 439, 532
BspFNI CGCG 2 cut(s) 35, 41
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 12, 564
BspMAI CTGCAG 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 2 cut(s) 7, 203
BsrI ACTGG 2 cut(s) 21, 298
BssAI RCCGGY 2 cut(s) 7, 203
BssECI CCNNGG 5 cut(s) 27, 33, 39, 555, 597
BssNI GRCGYC 2 cut(s) 126, 201
Bst6I CTCTTC 4 cut(s) 45, 245, 528, 534
BstACI GRCGYC 2 cut(s) 126, 201
BstDEI CTNAG 3 cut(s) 426, 519, 567
BstDSI CCRYGG 2 cut(s) 33, 39
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 2 cut(s) 35, 41
BstH2I RGCGCY 1 cut(s) 660
BstHHI GCGC 1 cut(s) 659
BstMAI GTCTC 1 cut(s) 64
BstMCI CGRYCG 2 cut(s) 39, 45
BstMWI GCNNNNNNNGC 4 cut(s) 38, 41, 44, 623
BstPAI GACNNNNGTC 1 cut(s) 204
BstSCI CCNGG 2 cut(s) 554, 596
BstSFI CTRYAG 1 cut(s) 431
BstUI CGCG 2 cut(s) 35, 41
BstV1I GCAGC 4 cut(s) 417, 430, 496, 518
BstV2I GAAGAC 2 cut(s) 204, 326
BstZI CGGCCG 2 cut(s) 36, 42
Bsu36I CCTNAGG 2 cut(s) 519, 567
BsuRI GGCC 5 cut(s) 32, 38, 44, 404, 596
BtgI CCRYGG 2 cut(s) 33, 39
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 14
CciI TCATGA 1 cut(s) 585
CciNI GCGGCCGC 2 cut(s) 36, 42
CfoI GCGC 1 cut(s) 659
Cfr10I RCCGGY 2 cut(s) 7, 203
Cfr13I GGNCC 1 cut(s) 515
Cfr42I CCGCGG 2 cut(s) 36, 42
CseI GACGC 1 cut(s) 209
CviAII CATG 3 cut(s) 469, 586, 664
DdeI CTNAG 3 cut(s) 426, 519, 567
EaeI YGGCCR 2 cut(s) 36, 42
EagI CGGCCG 2 cut(s) 36, 42
Eam1104I CTCTTC 4 cut(s) 45, 245, 528, 534
EarI CTCTTC 4 cut(s) 45, 245, 528, 534
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 2 cut(s) 423, 670
EclXI CGGCCG 2 cut(s) 36, 42
Eco24I GRGCYC 2 cut(s) 425, 672
Eco47I GGWCC 1 cut(s) 515
Eco47III AGCGCT 1 cut(s) 658
Eco52I CGGCCG 2 cut(s) 36, 42
Eco53kI GAGCTC 2 cut(s) 423, 670
Eco57I CTGAAG 1 cut(s) 290
Eco81I CCTNAGG 2 cut(s) 519, 567
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 2 cut(s) 423, 670
EcoT38I GRGCYC 2 cut(s) 425, 672
FaeI CATG 3 cut(s) 472, 589, 667
FaiI YATR 6 cut(s) 281, 332, 387, 470, 587, 665
FaqI GGGAC 1 cut(s) 659
FatI CATG 3 cut(s) 468, 585, 663
FauI CCCGC 1 cut(s) 635
Fnu4HI GCNGC 9 cut(s) 33, 36, 39, 42, 45, 431, 444, 507, 510
FokI GGATG 1 cut(s) 472
FriOI GRGCYC 2 cut(s) 425, 672
Fsp4HI GCNGC 9 cut(s) 33, 36, 39, 42, 45, 431, 444, 507, 510
GlaI GCGC 1 cut(s) 658
GluI GCNGC 9 cut(s) 33, 36, 39, 42, 45, 431, 444, 507, 510
GsaI CCCAGC 2 cut(s) 278, 451
GsuI CTGGAG 1 cut(s) 522
HaeII RGCGCY 1 cut(s) 660
HaeIII GGCC 5 cut(s) 32, 38, 44, 404, 596
HapII CCGG 6 cut(s) 8, 204, 464, 513, 555, 597
HgaI GACGC 1 cut(s) 209
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 2 cut(s) 126, 201
Hin1II CATG 3 cut(s) 472, 589, 667
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HinfI GANTC 4 cut(s) 221, 253, 367, 634
HpaII CCGG 6 cut(s) 8, 204, 464, 513, 555, 597
HphI GGTGA 1 cut(s) 664
Hpy188I TCNGA 3 cut(s) 93, 258, 379
Hpy188III TCNNGA 8 cut(s) 79, 316, 346, 364, 457, 501, 586, 631
Hpy99I CGWCG 2 cut(s) 140, 185
HpyAV CCTTC 2 cut(s) 253, 370
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 1 cut(s) 433
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 41, 44, 623
HpyF3I CTNAG 3 cut(s) 426, 519, 567
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 2 cut(s) 126, 201
Hsp92II CATG 3 cut(s) 472, 589, 667
HspAI GCGC 1 cut(s) 657
KspI CCGCGG 2 cut(s) 36, 42
LmnI GCTCC 4 cut(s) 16, 503, 609, 667
Lsp1109I GCAGC 4 cut(s) 417, 430, 496, 518
LweI GCATC 2 cut(s) 346, 450
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 3 cut(s) 3, 289, 307
MhlI GDGCHC 2 cut(s) 425, 672
MlyI GAGTC 4 cut(s) 230, 262, 376, 628
MmeI TCCRAC 1 cut(s) 236
MseI TTAA 1 cut(s) 679
MspA1I CMGCKG 5 cut(s) 35, 41, 430, 509, 642
MspI CCGG 6 cut(s) 8, 204, 464, 513, 555, 597
MspR9I CCNGG 2 cut(s) 556, 598
MvnI CGCG 2 cut(s) 35, 41
MwoI GCNNNNNNNGC 4 cut(s) 38, 41, 44, 623
NciI CCSGG 2 cut(s) 556, 598
NlaIII CATG 3 cut(s) 472, 589, 667
NlaIV GGNNCC 2 cut(s) 12, 564
NmuCI GTSAC 1 cut(s) 3
NotI GCGGCCGC 2 cut(s) 36, 42
PagI TCATGA 1 cut(s) 585
PcsI WCGNNNNNNNCGW 4 cut(s) 132, 144, 165, 180
PflFI GACNNNGTC 1 cut(s) 650
PkrI GCNGC 9 cut(s) 34, 37, 40, 43, 46, 432, 445, 508, 511
PleI GAGTC 4 cut(s) 229, 261, 375, 628
PpsI GAGTC 4 cut(s) 229, 261, 375, 628
PshAI GACNNNNGTC 1 cut(s) 204
Psp124BI GAGCTC 2 cut(s) 425, 672
PspFI CCCAGC 2 cut(s) 274, 447
PspN4I GGNNCC 2 cut(s) 12, 564
PspPI GGNCC 1 cut(s) 515
PstI CTGCAG 1 cut(s) 435
PsyI GACNNNGTC 1 cut(s) 650
PvuII CAGCTG 2 cut(s) 430, 509
SacI GAGCTC 2 cut(s) 425, 672
SacII CCGCGG 2 cut(s) 36, 42
SaqAI TTAA 1 cut(s) 679
SatI GCNGC 9 cut(s) 33, 36, 39, 42, 45, 431, 444, 507, 510
Sau96I GGNCC 1 cut(s) 515
SchI GAGTC 4 cut(s) 230, 262, 376, 628
ScrFI CCNGG 2 cut(s) 556, 598
SduI GDGCHC 2 cut(s) 425, 672
SfaNI GCATC 2 cut(s) 346, 450
SfcI CTRYAG 1 cut(s) 431
Sfr303I CCGCGG 2 cut(s) 36, 42
SgrBI CCGCGG 2 cut(s) 36, 42
SinI GGWCC 1 cut(s) 515
SmlI CTYRAG 2 cut(s) 77, 629
SmoI CTYRAG 2 cut(s) 77, 629
SsiI CCGC 7 cut(s) 33, 35, 39, 41, 45, 63, 642
SstI GAGCTC 2 cut(s) 425, 672
StyD4I CCNGG 2 cut(s) 554, 596
TaiI ACGT 1 cut(s) 129
TaqI TCGA 3 cut(s) 120, 227, 347
TauI GCSGC 5 cut(s) 35, 38, 41, 44, 47
Tru1I TTAA 1 cut(s) 679
Tru9I TTAA 1 cut(s) 679
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 430, 443, 506, 509
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 3 cut(s) 296, 543, 564
TspRI CASTG 1 cut(s) 21
Tth111I GACNNNGTC 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 515
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.