pycom02g05960

DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004053
Physical Location & Seq
Reverse (-)
4017822 .. 4019667
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1077 bp
ATGGAAGCCGGCAATTCGACCGTTGCCGTGTCAAGGTTGAAGAGGGAAGATTGCGAGCGCCCCAAAAACGACTCTCGCTTCTCCAATTGGAAGATATTAGTTGGCCCTTTTGATTGGGAAGACCATTCGCTGGGCAAAGAAGGAGCTGAAAGATACAGAGTTCATAACCTCCCCAAAAAGGAGAGTCCCGGTGTCTATGAACTTGGGGTTTCCGGTTGTAGCAGCCTAGGCCGTGAGAGTGGGAAGCTTGACCCAGGCTGCGTAGTCCCGGTGTACCTTGGACAAGCTGATAATGCGAGGACTCGACTTCAGCAATACGGTCGTTCAGGTGCTCATTTGGGCAATGGCAGTTCAACTGATCATCCTACTGACTGTGTCCGAAGGGAAATGGGGCGCGGATTGTTTGAGGACATACTGTCAAGAGGCTACCCCATTGTGTATAGATGGGCTCCTATGGAAAACAAAAGCGACGCACTGAAAACAGAAAATCAACTGCTTGATACATATGATTATGCATGGAATAGAACCAATAATGGAGCAAGGCGCCCTGATGATGTCCATCGAAAACTCAAGAATATATCTTCAAGCACTACGCAACGTACTGACATTGCCCAGGAGCTTCTTCCTTCCAGTCAGAAGAAAGTGGGCATCAGAATTGAGTCAAGCAAACCAATTTCAACGGGTGACAAATTCGGTGCTTATGATGACAGGGAGATCCACAACTCCCCTTGTCGGGTCTTCAAGCTCGGCAGAGCACAGCCTAGGTTGGTTTCGAATAGAAGCATTACTGAGGAGAATACTAGTATAATTTGTGGGGCGGATGTAGGAGATGGTTCTGTTTGCAGAACAGCACCAGTTCCCGGAAGACAGAGGTGTGCTGAACACAAAGGGATGAGGGCTAAGGGGTCAACCAGAGTTGGGATATCAAACAGCAATGTCGACTCACAGTACCGCTTTATCAGCAACCATGATTCAGTAGAGCCTTGCGGATTCATCTTGGCTGATGGATCGCCTTGTGGAAGGCATCCTATTCAAGGGCGGAAAAGGTGTAATCAGCATAAAGGGAGAAGAATTTAG

Protein Analysis

359

Amino Acids

39.8

Weight (kDa)

9.34

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIY_YIG_domain PF19239 27 - 186 1.2e-72 GIY-YIG domain-containing protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 543
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 1 cut(s) 939
AccII CGCG 1 cut(s) 396
AciI CCGC 5 cut(s) 396, 818, 952, 987, 1039
AclWI GGATC 2 cut(s) 709, 1015
AcsI RAATTY 2 cut(s) 689, 1071
AcuI CTGAAG 1 cut(s) 293
AcyI GRCGYC 1 cut(s) 544
AfaI GTAC 3 cut(s) 275, 601, 950
AfiI CCNNNNNNNGG 8 cut(s) 33, 130, 178, 732, 733, 860, 918, 1034
AgsI TTSAA 6 cut(s) 40, 354, 585, 678, 742, 1034
AhdI GACNNNNNGTC 1 cut(s) 415
AhlI ACTAGT 1 cut(s) 800
AjnI CCWGG 2 cut(s) 253, 612
AluBI AGCT 5 cut(s) 146, 247, 287, 619, 745
AluI AGCT 5 cut(s) 146, 247, 287, 619, 745
Alw21I GWGCWC 2 cut(s) 334, 757
AlwI GGATC 2 cut(s) 709, 1015
AoxI GGCC 2 cut(s) 103, 229
ApeKI GCWGC 2 cut(s) 222, 258
ApoI RAATTY 2 cut(s) 689, 1071
AspA2I CCTAGG 2 cut(s) 226, 761
AspLEI GCGC 3 cut(s) 60, 396, 546
AspS9I GGNCC 1 cut(s) 104
AsuC2I CCSGG 3 cut(s) 189, 269, 861
AsuHPI GGTGA 1 cut(s) 695
AsuII TTCGAA 1 cut(s) 773
AvrII CCTAGG 2 cut(s) 226, 761
BanI GGYRCC 1 cut(s) 543
BanII GRGCYC 1 cut(s) 451
BbsI GAAGAC 3 cut(s) 126, 730, 871
Bbv12I GWGCWC 2 cut(s) 334, 757
BbvI GCAGC 2 cut(s) 234, 245
BccI CCATC 4 cut(s) 438, 567, 824, 998
BceAI ACGGC 2 cut(s) 11, 216
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 2 cut(s) 255, 614
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 3 cut(s) 189, 269, 861
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 3 cut(s) 227, 762, 801
BfoI RGCGCY 2 cut(s) 61, 547
BisI GCNGC 2 cut(s) 223, 259
BlnI CCTAGG 2 cut(s) 226, 761
BlsI GCNGC 2 cut(s) 224, 260
Bme1390I CCNGG 5 cut(s) 189, 255, 269, 614, 861
BmeRI GACNNNNNGTC 1 cut(s) 415
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 2 cut(s) 450, 545
BmrFI CCNGG 5 cut(s) 189, 255, 269, 614, 861
BmsI GCATC 2 cut(s) 657, 1033
BpiI GAAGAC 3 cut(s) 126, 730, 871
Bpu10I CCTNAGC 1 cut(s) 900
Bpu14I TTCGAA 1 cut(s) 773
BpuEI CTTGAG 1 cut(s) 554
BpuMI CCSGG 3 cut(s) 189, 269, 861
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 544
BsaJI CCNNGG 5 cut(s) 226, 253, 277, 612, 761
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 8 cut(s) 33, 130, 178, 732, 733, 860, 918, 1034
Bse118I RCCGGY 1 cut(s) 8
Bse1I ACTGG 2 cut(s) 630, 854
Bse3DI GCAATG 3 cut(s) 349, 606, 940
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 2 cut(s) 255, 614
BseDI CCNNGG 5 cut(s) 226, 253, 277, 612, 761
BseGI GGATG 4 cut(s) 361, 826, 897, 1024
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 8 cut(s) 33, 130, 178, 732, 733, 860, 918, 1034
BseMI GCAATG 3 cut(s) 349, 606, 940
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 2 cut(s) 630, 854
BseRI GAGGAG 1 cut(s) 806
BseXI GCAGC 2 cut(s) 234, 245
BseYI CCCAGC 1 cut(s) 130
Bsh1236I CGCG 1 cut(s) 396
Bsh1285I CGRYCG 2 cut(s) 21, 322
BshFI GGCC 2 cut(s) 105, 231
BshNI GGYRCC 1 cut(s) 543
BsiEI CGRYCG 2 cut(s) 21, 322
BsiHKAI GWGCWC 2 cut(s) 334, 757
BsiSI CCGG 5 cut(s) 9, 189, 213, 269, 861
BslFI GGGAC 2 cut(s) 171, 251
BslI CCNNNNNNNGG 8 cut(s) 33, 130, 178, 732, 733, 860, 918, 1034
BsmFI GGGAC 2 cut(s) 171, 251
BsnI GGCC 2 cut(s) 105, 231
Bsp119I TTCGAA 1 cut(s) 773
Bsp1286I GDGCHC 3 cut(s) 334, 451, 757
Bsp143I GATC 3 cut(s) 358, 714, 1007
BspACI CCGC 5 cut(s) 396, 818, 952, 987, 1039
BspANI GGCC 2 cut(s) 105, 231
BspCNI CTCAG 1 cut(s) 781
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 2 cut(s) 450, 545
BspPI GGATC 2 cut(s) 709, 1015
BspT104I TTCGAA 1 cut(s) 773
BspT107I GGYRCC 1 cut(s) 543
BsrDI GCAATG 3 cut(s) 349, 606, 940
BsrFI RCCGGY 1 cut(s) 8
BsrI ACTGG 2 cut(s) 630, 854
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 5 cut(s) 226, 253, 277, 612, 761
BssMI GATC 3 cut(s) 358, 714, 1007
BssNI GRCGYC 1 cut(s) 544
BssT1I CCWWGG 3 cut(s) 226, 277, 761
Bst2UI CCWGG 2 cut(s) 255, 614
Bst4CI ACNGT 5 cut(s) 22, 320, 374, 417, 948
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 544
BstBI TTCGAA 1 cut(s) 773
BstC8I GCNNGC 2 cut(s) 10, 56
BstDEI CTNAG 2 cut(s) 789, 900
BstENI CCTNNNNNAGG 1 cut(s) 1032
BstF5I GGATG 4 cut(s) 361, 826, 897, 1024
BstFNI CGCG 1 cut(s) 396
BstH2I RGCGCY 2 cut(s) 61, 547
BstHHI GCGC 3 cut(s) 60, 396, 546
BstKTI GATC 3 cut(s) 361, 717, 1010
BstMBI GATC 3 cut(s) 358, 714, 1007
BstMCI CGRYCG 2 cut(s) 21, 322
BstMWI GCNNNNNNNGC 3 cut(s) 228, 293, 960
BstNI CCWGG 2 cut(s) 255, 614
BstSCI CCNGG 5 cut(s) 187, 253, 267, 612, 859
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 2 cut(s) 234, 245
BstV2I GAAGAC 3 cut(s) 126, 730, 871
BstX2I RGATCY 1 cut(s) 714
BstYI RGATCY 1 cut(s) 714
BsuRI GGCC 2 cut(s) 105, 231
BtsCI GGATG 4 cut(s) 361, 826, 897, 1024
BtsIMutI CAGTG 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 10, 56
CfoI GCGC 3 cut(s) 60, 396, 546
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 1 cut(s) 104
CseI GACGC 1 cut(s) 479
Csp6I GTAC 3 cut(s) 274, 600, 949
CviAII CATG 2 cut(s) 516, 968
CviQI GTAC 3 cut(s) 274, 600, 949
DdeI CTNAG 2 cut(s) 789, 900
DinI GGCGCC 1 cut(s) 545
DpnI GATC 3 cut(s) 360, 716, 1009
DpnII GATC 3 cut(s) 358, 714, 1007
DriI GACNNNNNGTC 1 cut(s) 415
Eam1104I CTCTTC 1 cut(s) 35
Eam1105I GACNNNNNGTC 1 cut(s) 415
EarI CTCTTC 1 cut(s) 35
EciI GGCGGA 2 cut(s) 833, 1054
Eco130I CCWWGG 3 cut(s) 226, 277, 761
Eco24I GRGCYC 1 cut(s) 451
Eco32I GATATC 1 cut(s) 924
Eco57I CTGAAG 1 cut(s) 293
EcoNI CCTNNNNNAGG 1 cut(s) 1032
EcoRII CCWGG 2 cut(s) 253, 612
EcoRV GATATC 1 cut(s) 924
EcoT14I CCWWGG 3 cut(s) 226, 277, 761
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 451
EgeI GGCGCC 1 cut(s) 545
EheI GGCGCC 1 cut(s) 545
ErhI CCWWGG 3 cut(s) 226, 277, 761
FaeI CATG 2 cut(s) 519, 971
FaqI GGGAC 2 cut(s) 171, 251
FatI CATG 2 cut(s) 515, 967
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 1 cut(s) 939
Fnu4HI GCNGC 2 cut(s) 223, 259
FokI GGATG 4 cut(s) 348, 833, 904, 1011
FriOI GRGCYC 1 cut(s) 451
Fsp4HI GCNGC 2 cut(s) 223, 259
FspBI CTAG 3 cut(s) 227, 762, 801
GlaI GCGC 3 cut(s) 59, 395, 545
GluI GCNGC 2 cut(s) 223, 259
GsaI CCCAGC 1 cut(s) 134
HaeII RGCGCY 2 cut(s) 61, 547
HaeIII GGCC 2 cut(s) 105, 231
HapII CCGG 5 cut(s) 9, 189, 213, 269, 861
HgaI GACGC 1 cut(s) 479
HhaI GCGC 3 cut(s) 60, 396, 546
Hin1I GRCGYC 1 cut(s) 544
Hin1II CATG 2 cut(s) 519, 971
Hin6I GCGC 3 cut(s) 58, 394, 544
HinP1I GCGC 3 cut(s) 58, 394, 544
HincII GTYRAC 2 cut(s) 909, 940
HindII GTYRAC 2 cut(s) 909, 940
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 7 cut(s) 71, 184, 301, 659, 941, 971, 990
HpaII CCGG 5 cut(s) 9, 189, 213, 269, 861
HphI GGTGA 1 cut(s) 695
Hpy166II GTNNAC 3 cut(s) 274, 909, 940
Hpy188I TCNGA 3 cut(s) 380, 636, 653
Hpy188III TCNNGA 2 cut(s) 420, 571
Hpy8I GTNNAC 3 cut(s) 274, 909, 940
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 4 cut(s) 134, 375, 636, 1014
HpyCH4III ACNGT 5 cut(s) 22, 320, 374, 417, 948
HpyCH4IV ACGT 1 cut(s) 598
HpyCH4V TGCA 2 cut(s) 515, 843
HpyF10VI GCNNNNNNNGC 3 cut(s) 228, 293, 960
HpyF3I CTNAG 2 cut(s) 789, 900
HpySE526I ACGT 1 cut(s) 598
Hsp92I GRCGYC 1 cut(s) 544
Hsp92II CATG 2 cut(s) 519, 971
HspAI GCGC 3 cut(s) 58, 394, 544
KasI GGCGCC 1 cut(s) 543
KroI GCCGGC 1 cut(s) 8
KroNI GCCGGC 1 cut(s) 10
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 3 cut(s) 358, 714, 1007
LmnI GCTCC 4 cut(s) 143, 454, 536, 616
Lsp1109I GCAGC 2 cut(s) 234, 245
LweI GCATC 2 cut(s) 657, 1033
MaeI CTAG 3 cut(s) 227, 762, 801
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 1 cut(s) 683
MalI GATC 3 cut(s) 360, 716, 1009
MboI GATC 3 cut(s) 358, 714, 1007
MboII GAAGA 9 cut(s) 52, 59, 103, 131, 573, 614, 649, 730, 876
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 1 cut(s) 714
MhlI GDGCHC 3 cut(s) 334, 451, 757
MluCI AATT 7 cut(s) 13, 85, 654, 672, 689, 807, 1071
Mly113I GGCGCC 1 cut(s) 544
MlyI GAGTC 5 cut(s) 65, 193, 295, 668, 935
MnlI CCTC 8 cut(s) 36, 179, 291, 400, 416, 784, 864, 888
Mph1103I ATGCAT 1 cut(s) 517
MroNI GCCGGC 1 cut(s) 8
MspI CCGG 5 cut(s) 9, 189, 213, 269, 861
MspR9I CCNGG 5 cut(s) 189, 255, 269, 614, 861
MunI CAATTG 1 cut(s) 85
MvaI CCWGG 2 cut(s) 255, 614
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 3 cut(s) 228, 293, 960
NaeI GCCGGC 1 cut(s) 10
NarI GGCGCC 1 cut(s) 544
NciI CCSGG 3 cut(s) 189, 269, 861
NdeI CATATG 1 cut(s) 505
NdeII GATC 3 cut(s) 358, 714, 1007
NgoMIV GCCGGC 1 cut(s) 8
NlaIII CATG 2 cut(s) 519, 971
NlaIV GGNNCC 2 cut(s) 450, 545
NmeAIII GCCGAG 1 cut(s) 726
NmuCI GTSAC 1 cut(s) 683
NsiI ATGCAT 1 cut(s) 517
NspV TTCGAA 1 cut(s) 773
PdiI GCCGGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 971, 990
PflFI GACNNNGTC 1 cut(s) 374
PflMI CCANNNNNTGG 1 cut(s) 130
PfoI TCCNGGA 1 cut(s) 859
PkrI GCNGC 2 cut(s) 224, 260
PleI GAGTC 5 cut(s) 65, 192, 295, 667, 935
PluTI GGCGCC 1 cut(s) 547
PpsI GAGTC 5 cut(s) 65, 192, 295, 667, 935
Psp6I CCWGG 2 cut(s) 253, 612
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 2 cut(s) 253, 612
PspN4I GGNNCC 2 cut(s) 450, 545
PspPI GGNCC 1 cut(s) 104
PsuI RGATCY 1 cut(s) 714
PsyI GACNNNGTC 1 cut(s) 374
RsaI GTAC 3 cut(s) 275, 601, 950
RsaNI GTAC 3 cut(s) 274, 600, 949
SalI GTCGAC 1 cut(s) 938
SatI GCNGC 2 cut(s) 223, 259
Sau3AI GATC 3 cut(s) 358, 714, 1007
Sau96I GGNCC 1 cut(s) 104
SchI GAGTC 5 cut(s) 65, 193, 295, 668, 935
ScrFI CCNGG 5 cut(s) 189, 255, 269, 614, 861
SduI GDGCHC 3 cut(s) 334, 451, 757
SfaNI GCATC 2 cut(s) 657, 1033
SfoI GGCGCC 1 cut(s) 545
SfuI TTCGAA 1 cut(s) 773
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
SpeI ACTAGT 1 cut(s) 800
Sse9I AATT 7 cut(s) 13, 85, 654, 672, 689, 807, 1071
SsiI CCGC 5 cut(s) 396, 818, 952, 987, 1039
SspDI GGCGCC 1 cut(s) 543
SspMI CTAG 3 cut(s) 227, 762, 801
StyD4I CCNGG 5 cut(s) 187, 253, 267, 612, 859
StyI CCWWGG 3 cut(s) 226, 277, 761
TaaI ACNGT 5 cut(s) 22, 320, 374, 417, 948
TaiI ACGT 1 cut(s) 601
TaqI TCGA 5 cut(s) 17, 304, 562, 773, 939
TasI AATT 7 cut(s) 13, 85, 654, 672, 689, 807, 1071
TfiI GAWTC 2 cut(s) 971, 990
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 1 cut(s) 683
TseI GCWGC 2 cut(s) 222, 258
Tsp45I GTSAC 1 cut(s) 683
TspDTI ATGAA 3 cut(s) 152, 213, 982
TspRI CASTG 1 cut(s) 480
Tth111I GACNNNGTC 1 cut(s) 374
Van91I CCANNNNNTGG 1 cut(s) 130
XagI CCTNNNNNAGG 1 cut(s) 1032
XapI RAATTY 2 cut(s) 689, 1071
XmaJI CCTAGG 2 cut(s) 226, 761
XmiI GTMKAC 1 cut(s) 939
XspI CTAG 3 cut(s) 227, 762, 801
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.