pycom02g11750

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003343
Physical Location & Seq
Reverse (-)
8326562 .. 8332315
5754 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2334 bp
ATGCGTCAGACGCTTCTTCGCCTTCCCTCCTTTTGCTCTCTCCGCTTCCGGCCTCTGGAGCCCAGTCTCTCCGCGCTTCGCACTGAGAAGAAACGCTACCGCCATTTTGAAATGGCGACGAAGAACGATCCGCCGGCAGATTCTCCGACTTCAGTACTCGAGGACGAGGATGCATGTGAGACAAAGATCGACGTCAAGCTGACTGAGGTGGAGGAGAAATTGCTTGAAGAGCGGGTTAAGGAAGAGGAGTCAGAAACGGAAAAGGGACCAGAGCAGTTGCCCCACCTTAATGACACTCAGTATAACAAGTTGGATGAGCTCCTTACGCAAACTCAGCTGTACTCGCAGTTTTTGCTTGAGAAAATGGATAACATTACACTTGTTGGAGCCAATCAACCGAGTGAAACTGTTGAGGAAAAGAATGTTGAGGAAAAGAAAGGTCGTGGTAGGAAAAGAAAAGCAGCTGCCAATTTCGATAATAGGAAGGCCAAGAAGGCAGTTGAAGCTATGCTTACAAGATCTAAAGAGGGTGTGAAAATTGAAGACGAGAACCTCACTGAAGAACAAAGAATTGAAAAAGAACAAAAGGAACTTGTACCTCTGCTGACTGGTGGAAAGTTGAAGTCTTATCAAATCAAAGGTGTAAAGTGGTTGATCTCGTTATGGCAAAATGGTCTGAATGGGATCCTTGCAGATCAAATGGGACTTGGAAAAACTATCCAAACCATTGGTTTTCTTGCTCATCTAAAAGGGAATGGAATGGATGGGCCCTACTTGGTGATTGCTCCTCTTTCTACTCTTGCGAATTGGGTAAATGAAATTTCAAGGTTCACGCCTTCAATAAATGCTATTGTCTACCATGGTGGCAAGAAACAAAGGGATGAGATACGAAGGAAGCACATGCCTAGTAAAATTGGCCCTAAGTTCCCTATAATTGTTACTTCGTATGAAATTGCAATGGCTGATGCAAGAAGATGTTTAAGACATTACAGTTGGAAATATCTGGTGGTTGATGAAGGTCACAGATTGAAGAACTCAAAGTGCATACTGCTGCGGGAATTGAAACATTTACGTGTAGACAATAAGATTCTGTTGACTGGGACACCTCTACAGAATAATTTGGCAGAGCTTTGGTCATTATTAAACTTCATTTTGCCTGATATATTCTCATCCCATGAAGAATTCGAGTCATGGTTTGATTTGGCAGGAAAGTGCAGTAATGAGGCAATGAAGGAAGAATTGGAAGAGAAGAGAAGGGCTCAAATGGTTGCAAAACTCCATGCCATCTTGCGTCCTTTCCTTCTTCGAAGAATGAAGTCTGATGTTGAGCACATGCTTCCTAGGAAAAAGGAAATCATATTATATGCATGCATGACAGAGCATCAAAAACATTTTCAAGATCTTCTGATCAATAAGACATTGGAGAATTATTTACTTGAGAGGGGAGACCATGTTTGTGGTATGAAGGGAAAGCTTAACAATCTGATGGTCCAACTTCGGAAGAATTGCAATCATCCTGACCTTTTGGAGTCTGCATTTGATGGCTCATATTTCTACCCGCCTGTTGAACAGATTGTTGAACAGTGTGGAAAATTTAGTTTGCTGGACCGGCTGTTAAAGCACCTGTTTGCCCGCAAACACAAAGTTCTTATCTTCTCTCAGTGGACTAAGATTTTGGATATTATGGACTACTATTTTAGTGAGAAAGGATTAGAAGTTTGTAGAATTGACGGCAATGTGAAACTTGAAGATCGGAGAAATCAGATTGCAGTGTTCAACGACGTGGACAGCAATCATAGAATCTTTCTTCTAAGCACCAGAGCTGGTGGACTGGGTATCAACCTTACTGCAGCTGATACTTGTATACTGTACGACAGTGATTGGAACCCTCAAATGGATTTGCAAGCCATGGATAGATGTCACAGAATTGGTCAAACGAAGCCTGTTCATGTTTACAGGTTGGCAACAGCACAATCTATCGAGGGTCGGATTCTGAAAAGAGCTTTTAGCAAGTTGAAGCTTGAACATGTGGTGATTGGGAAAGGGCAATTCCATCAAGATAAAGCGAAGAGTAGTACTGATTTCCTGGAGGAAGAGGATCTTGTAGCATTGCTCCGAGAGGAAGAATCTGCTGAAGACAAGATGATACAGACAGTTATCAGTGATGAAGATTTGGAGAGGGTGTTGGATCGCAGTGATCTAATGGGCAGTCCTGCTGATCATGAAGAAGAGAAGGCCAATGGTGTTGCCGATGTTTTTCCCCTCAAAGGCCCGGGTTGGGAGGTGGTGCTGCCTACTGCAGGTGGAGGCATGCTCTCCACCCTCACTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000786 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006325 GO:0006342 GO:0006344 GO:0006346 GO:0006349 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016043 GO:0016458 GO:0016462 GO:0016569 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031056 GO:0031057 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031400 GO:0031401 GO:0032196 GO:0032197 GO:0032268 GO:0032269 GO:0032270 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034641 GO:0035065 GO:0035067 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044030 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044764 GO:0044815 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0060255 GO:0065007 GO:0070035 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090239 GO:0090241 GO:0090304 GO:0140097 GO:1901360 GO:1901983 GO:1901984 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1905269 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

778

Amino Acids

89.11

Weight (kDa)

6.67

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 206 - 369 4.1e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 210 - 508 9.9e-84 SNF2-related domain
DEAD PF00270 224 - 373 1.8e-07 DEAD/DEAH box helicase
HDA2-3 PF11496 453 - 666 9.3e-06 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 531 - 644 2.8e-25 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2291
AatII GACGTC 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 2291
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 3 cut(s) 855, 1077, 1864
AccII CGCG 1 cut(s) 74
AciI CCGC 8 cut(s) 43, 72, 100, 131, 232, 1054, 1559, 1633
AclWI GGATC 5 cut(s) 122, 679, 692, 2106, 2196
AcsI RAATTY 3 cut(s) 819, 1181, 1592
AcuI CTGAAG 3 cut(s) 135, 579, 2154
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 5 cut(s) 156, 341, 597, 1871, 2077
AfiI CCNNNNNNNGG 5 cut(s) 55, 1894, 2267, 2278, 2300
AflIII ACRYGT 2 cut(s) 1072, 2026
AhlI ACTAGT 1 cut(s) 2327
AjiI CACGTC 1 cut(s) 1783
AjnI CCWGG 1 cut(s) 2085
AjuI GAANNNNNNNTTGG 2 cut(s) 1223, 1255
Alw21I GWGCWC 2 cut(s) 321, 1332
Alw26I GTCTC 3 cut(s) 71, 173, 1440
AlwI GGATC 5 cut(s) 122, 679, 692, 2106, 2196
Ama87I CYCGRG 2 cut(s) 158, 2272
AoxI GGCC 6 cut(s) 50, 486, 767, 916, 2235, 2269
ApaI GGGCCC 1 cut(s) 771
ApeKI GCWGC 5 cut(s) 461, 464, 1051, 1850, 2290
ApoI RAATTY 3 cut(s) 819, 1181, 1592
ArsI GACNNNNNNTTYG 2 cut(s) 1657, 1689
AspA2I CCTAGG 1 cut(s) 1340
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 7 cut(s) 266, 767, 768, 917, 1489, 1606, 2270
AsuC2I CCSGG 2 cut(s) 2273, 2274
AsuHPI GGTGA 2 cut(s) 790, 2044
AsuII TTCGAA 1 cut(s) 1306
AvaI CYCGRG 2 cut(s) 158, 2272
AvaII GGWCC 3 cut(s) 266, 1489, 1606
AvrII CCTAGG 1 cut(s) 1340
BaeGI GKGCMC 1 cut(s) 771
BamHI GGATCC 1 cut(s) 684
BanII GRGCYC 4 cut(s) 63, 321, 771, 1261
BarI GAAGNNNNNNTAC 2 cut(s) 80, 112
BbsI GAAGAC 2 cut(s) 549, 2142
Bbv12I GWGCWC 2 cut(s) 321, 1332
BbvI GCAGC 5 cut(s) 451, 473, 1038, 1862, 2277
BccI CCATC 5 cut(s) 758, 1292, 1480, 1535, 2061
BceAI ACGGC 1 cut(s) 1747
BciT130I CCWGG 1 cut(s) 2087
BclI TGATCA 2 cut(s) 1407, 2218
BcnI CCSGG 2 cut(s) 2273, 2274
BcoDI GTCTC 3 cut(s) 71, 173, 1440
BcuI ACTAGT 1 cut(s) 2327
BfaI CTAG 3 cut(s) 906, 1341, 2328
BfmI CTRYAG 3 cut(s) 1109, 1848, 2298
BfuAI ACCTGC 1 cut(s) 2291
BglI GCCNNNNNGGC 1 cut(s) 494
BglII AGATCT 2 cut(s) 518, 1399
BisI GCNGC 5 cut(s) 462, 465, 1052, 1851, 2291
BlnI CCTAGG 1 cut(s) 1340
BlsI GCNGC 5 cut(s) 463, 466, 1053, 1852, 2292
BmcAI AGTACT 2 cut(s) 156, 2077
Bme1390I CCNGG 3 cut(s) 2087, 2273, 2274
Bme18I GGWCC 3 cut(s) 266, 1489, 1606
BmeT110I CYCGRG 2 cut(s) 158, 2272
BmgBI CACGTC 1 cut(s) 1783
BmgT120I GGNCC 7 cut(s) 266, 767, 768, 917, 1489, 1606, 2270
BmiI GGNNCC 6 cut(s) 60, 267, 388, 686, 769, 1886
BmrFI CCNGG 3 cut(s) 2087, 2273, 2274
BmrI ACTGGG 3 cut(s) 57, 1107, 1841
BmsI GCATC 3 cut(s) 160, 955, 1390
BmuI ACTGGG 3 cut(s) 57, 1107, 1841
BpiI GAAGAC 2 cut(s) 549, 2142
BplI GAGNNNNNCTC 4 cut(s) 1243, 1275, 2298, 2330
BpmI CTGGAG 2 cut(s) 77, 2108
Bpu14I TTCGAA 1 cut(s) 1306
BpuEI CTTGAG 2 cut(s) 377, 1457
BpuMI CCSGG 2 cut(s) 2273, 2274
BsaAI YACGTR 1 cut(s) 1073
BsaHI GRCGYC 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 1440
BsaJI CCNNGG 4 cut(s) 859, 1340, 1908, 2272
BsaXI ACNNNNNCTCC 4 cut(s) 1437, 1467, 2168, 2198
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 1894, 2267, 2278, 2300
Bse118I RCCGGY 2 cut(s) 133, 1608
Bse1I ACTGG 4 cut(s) 63, 613, 1102, 1836
Bse3DI GCAATG 4 cut(s) 963, 1233, 1741, 2108
BseBI CCWGG 1 cut(s) 2087
BseDI CCNNGG 4 cut(s) 859, 1340, 1908, 2272
BseGI GGATG 6 cut(s) 175, 319, 769, 886, 1169, 1513
BseLI CCNNNNNNNGG 5 cut(s) 55, 1894, 2267, 2278, 2300
BseMI GCAATG 4 cut(s) 963, 1233, 1741, 2108
BseMII CTCAG 5 cut(s) 75, 195, 311, 347, 1673
BseNI ACTGG 4 cut(s) 63, 613, 1102, 1836
BseRI GAGGAG 3 cut(s) 227, 260, 777
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 5 cut(s) 451, 473, 1038, 1862, 2277
BsgI GTGCAG 1 cut(s) 1234
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 6 cut(s) 52, 488, 769, 918, 2237, 2271
BsiHKAI GWGCWC 2 cut(s) 321, 1332
BsiHKCI CYCGRG 2 cut(s) 158, 2272
BsiSI CCGG 4 cut(s) 49, 134, 1609, 2273
BslFI GGGAC 3 cut(s) 279, 717, 1114
BslI CCNNNNNNNGG 5 cut(s) 55, 1894, 2267, 2278, 2300
BsmAI GTCTC 3 cut(s) 71, 173, 1440
BsmFI GGGAC 3 cut(s) 279, 717, 1114
BsnI GGCC 6 cut(s) 52, 488, 769, 918, 2237, 2271
Bso31I GGTCTC 1 cut(s) 1440
BsoBI CYCGRG 2 cut(s) 158, 2272
Bsp119I TTCGAA 1 cut(s) 1306
Bsp120I GGGCCC 1 cut(s) 767
Bsp1286I GDGCHC 5 cut(s) 63, 321, 771, 1261, 1332
Bsp19I CCATGG 2 cut(s) 859, 1908
BspACI CCGC 8 cut(s) 43, 72, 100, 131, 232, 1054, 1559, 1633
BspANI GGCC 6 cut(s) 52, 488, 769, 918, 2237, 2271
BspCNI CTCAG 5 cut(s) 76, 196, 310, 346, 1672
BspFNI CGCG 1 cut(s) 74
BspHI TCATGA 1 cut(s) 2221
BspLI GGNNCC 6 cut(s) 60, 267, 388, 686, 769, 1886
BspMAI CTGCAG 2 cut(s) 1852, 2302
BspMI ACCTGC 1 cut(s) 2291
BspPI GGATC 5 cut(s) 122, 679, 692, 2106, 2196
BspQI GCTCTTC 1 cut(s) 222
BspT104I TTCGAA 1 cut(s) 1306
BspTNI GGTCTC 1 cut(s) 1440
BsrBI CCGCTC 1 cut(s) 232
BsrDI GCAATG 4 cut(s) 963, 1233, 1741, 2108
BsrFI RCCGGY 2 cut(s) 133, 1608
BsrI ACTGG 4 cut(s) 63, 613, 1102, 1836
BssAI RCCGGY 2 cut(s) 133, 1608
BssECI CCNNGG 4 cut(s) 859, 1340, 1908, 2272
BssNAI GTATAC 1 cut(s) 1865
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 3 cut(s) 859, 1340, 1908
Bst1107I GTATAC 1 cut(s) 1865
Bst2UI CCWGG 1 cut(s) 2087
Bst4CI ACNGT 6 cut(s) 409, 992, 1584, 1869, 1877, 2155
Bst6I CTCTTC 7 cut(s) 222, 237, 1239, 1244, 2063, 2088, 2223
BstACI GRCGYC 1 cut(s) 192
BstAPI GCANNNNNTGC 1 cut(s) 352
BstBAI YACGTR 1 cut(s) 1073
BstBI TTCGAA 1 cut(s) 1306
BstC8I GCNNGC 5 cut(s) 135, 1369, 1633, 1905, 2312
BstDEI CTNAG 8 cut(s) 84, 204, 297, 333, 921, 1659, 1668, 1811
BstDSI CCRYGG 2 cut(s) 859, 1908
BstENI CCTNNNNNAGG 1 cut(s) 2298
BstF5I GGATG 6 cut(s) 175, 319, 769, 886, 1169, 1513
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 76
BstMAI GTCTC 3 cut(s) 71, 173, 1440
BstNI CCWGG 1 cut(s) 2087
BstNSI RCATGY 6 cut(s) 177, 904, 1336, 1371, 2030, 2314
BstSCI CCNGG 3 cut(s) 2085, 2271, 2272
BstSFI CTRYAG 3 cut(s) 1109, 1848, 2298
BstSLI GKGCMC 1 cut(s) 771
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 5 cut(s) 451, 473, 1038, 1862, 2277
BstV2I GAAGAC 2 cut(s) 549, 2142
BstX2I RGATCY 4 cut(s) 518, 684, 1399, 2098
BstXI CCANNNNNNTGG 2 cut(s) 728, 1457
BstYI RGATCY 4 cut(s) 518, 684, 1399, 2098
BstZ17I GTATAC 1 cut(s) 1865
BsuRI GGCC 6 cut(s) 52, 488, 769, 918, 2237, 2271
BtgI CCRYGG 2 cut(s) 859, 1908
BtrI CACGTC 1 cut(s) 1783
BtsCI GGATG 6 cut(s) 175, 319, 769, 886, 1169, 1513
BtsI GCAGTG 2 cut(s) 1776, 2200
BtsIMutI CAGTG 8 cut(s) 81, 555, 1589, 1667, 1776, 1882, 2167, 2200
BveI ACCTGC 1 cut(s) 2291
Cac8I GCNNGC 5 cut(s) 135, 1369, 1633, 1905, 2312
CciI TCATGA 1 cut(s) 2221
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 2 cut(s) 133, 1608
Cfr13I GGNCC 7 cut(s) 266, 767, 768, 917, 1489, 1606, 2270
Cfr9I CCCGGG 1 cut(s) 2272
CseI GACGC 2 cut(s) 19, 1280
Csp6I GTAC 5 cut(s) 155, 340, 596, 1870, 2076
CviQI GTAC 5 cut(s) 155, 340, 596, 1870, 2076
DdeI CTNAG 8 cut(s) 84, 204, 297, 333, 921, 1659, 1668, 1811
Eam1104I CTCTTC 7 cut(s) 222, 237, 1239, 1244, 2063, 2088, 2223
EarI CTCTTC 7 cut(s) 222, 237, 1239, 1244, 2063, 2088, 2223
EciI GGCGGA 1 cut(s) 120
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 3 cut(s) 859, 1340, 1908
Eco24I GRGCYC 4 cut(s) 63, 321, 771, 1261
Eco31I GGTCTC 1 cut(s) 1440
Eco47I GGWCC 3 cut(s) 266, 1489, 1606
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 3 cut(s) 135, 579, 2154
Eco88I CYCGRG 2 cut(s) 158, 2272
EcoICRI GAGCTC 1 cut(s) 319
EcoNI CCTNNNNNAGG 1 cut(s) 2298
EcoO109I RGGNCCY 1 cut(s) 768
EcoRI GAATTC 1 cut(s) 1181
EcoRII CCWGG 1 cut(s) 2085
EcoT14I CCWWGG 3 cut(s) 859, 1340, 1908
EcoT22I ATGCAT 3 cut(s) 175, 1369, 1373
EcoT38I GRGCYC 4 cut(s) 63, 321, 771, 1261
ErhI CCWWGG 3 cut(s) 859, 1340, 1908
FalI AAGNNNNNCTT 4 cut(s) 495, 527, 2085, 2117
FaqI GGGAC 3 cut(s) 279, 717, 1114
FauI CCCGC 4 cut(s) 225, 1047, 1566, 1640
FbaI TGATCA 2 cut(s) 1407, 2218
FblI GTMKAC 3 cut(s) 855, 1077, 1864
Fnu4HI GCNGC 5 cut(s) 462, 465, 1052, 1851, 2291
FokI GGATG 6 cut(s) 182, 326, 776, 893, 1156, 1500
FriOI GRGCYC 4 cut(s) 63, 321, 771, 1261
Fsp4HI GCNGC 5 cut(s) 462, 465, 1052, 1851, 2291
FspBI CTAG 3 cut(s) 906, 1341, 2328
GlaI GCGC 1 cut(s) 75
GluI GCNGC 5 cut(s) 462, 465, 1052, 1851, 2291
GsuI CTGGAG 2 cut(s) 77, 2108
HaeIII GGCC 6 cut(s) 52, 488, 769, 918, 2237, 2271
HapII CCGG 4 cut(s) 49, 134, 1609, 2273
HgaI GACGC 2 cut(s) 19, 1280
HhaI GCGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 192
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 2 cut(s) 1472, 2018
HinfI GANTC 8 cut(s) 140, 248, 1087, 1187, 1529, 1800, 1990, 2126
HpaII CCGG 4 cut(s) 49, 134, 1609, 2273
HphI GGTGA 2 cut(s) 790, 2044
Hpy166II GTNNAC 9 cut(s) 831, 856, 1078, 1095, 1665, 1786, 1829, 1865, 1954
Hpy188III TCNNGA 5 cut(s) 56, 1397, 1517, 2057, 2222
Hpy8I GTNNAC 9 cut(s) 831, 856, 1078, 1095, 1665, 1786, 1829, 1865, 1954
Hpy99I CGWCG 3 cut(s) 121, 194, 1784
HpyCH4III ACNGT 6 cut(s) 409, 992, 1584, 1869, 1877, 2155
HpyCH4IV ACGT 3 cut(s) 192, 1072, 1782
HpyF3I CTNAG 8 cut(s) 84, 204, 297, 333, 921, 1659, 1668, 1811
HpySE526I ACGT 3 cut(s) 192, 1072, 1782
Hsp92I GRCGYC 1 cut(s) 192
HspAI GCGC 1 cut(s) 74
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Ksp22I TGATCA 2 cut(s) 1407, 2218
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 5 cut(s) 58, 324, 386, 790, 2118
Lsp1109I GCAGC 5 cut(s) 451, 473, 1038, 1862, 2277
LweI GCATC 3 cut(s) 160, 955, 1390
MaeI CTAG 3 cut(s) 906, 1341, 2328
MaeII ACGT 3 cut(s) 192, 1072, 1782
MaeIII GTNAC 3 cut(s) 937, 1019, 1919
MbiI CCGCTC 1 cut(s) 232
MflI RGATCY 4 cut(s) 518, 684, 1399, 2098
MhlI GDGCHC 5 cut(s) 63, 321, 771, 1261, 1332
MlyI GAGTC 3 cut(s) 257, 1196, 1538
MmeI TCCRAC 7 cut(s) 170, 291, 364, 974, 1516, 1967, 2166
Mph1103I ATGCAT 3 cut(s) 175, 1369, 1373
MroNI GCCGGC 1 cut(s) 133
MseI TTAA 7 cut(s) 237, 288, 980, 1142, 1476, 1616, 2332
MslI CAYNNNNRTG 3 cut(s) 288, 1071, 1455
MspA1I CMGCKG 3 cut(s) 337, 464, 1853
MspI CCGG 4 cut(s) 49, 134, 1609, 2273
MspR9I CCNGG 3 cut(s) 2087, 2273, 2274
MvaI CCWGG 1 cut(s) 2087
MvnI CGCG 1 cut(s) 74
NaeI GCCGGC 1 cut(s) 135
NciI CCSGG 2 cut(s) 2273, 2274
NcoI CCATGG 2 cut(s) 859, 1908
NgoMIV GCCGGC 1 cut(s) 133
NlaIV GGNNCC 6 cut(s) 60, 267, 388, 686, 769, 1886
NmuCI GTSAC 2 cut(s) 1019, 1919
NsiI ATGCAT 3 cut(s) 175, 1369, 1373
NspI RCATGY 6 cut(s) 177, 904, 1336, 1371, 2030, 2314
NspV TTCGAA 1 cut(s) 1306
PaeI GCATGC 2 cut(s) 1371, 2314
PaeR7I CTCGAG 1 cut(s) 158
PagI TCATGA 1 cut(s) 2221
PaqCI CACCTGC 1 cut(s) 2291
PciI ACATGT 1 cut(s) 2026
PciSI GCTCTTC 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 135
PfeI GAWTC 5 cut(s) 140, 1087, 1800, 1990, 2126
PfoI TCCNGGA 1 cut(s) 2085
PkrI GCNGC 5 cut(s) 463, 466, 1053, 1852, 2292
PleI GAGTC 3 cut(s) 256, 1195, 1537
PpsI GAGTC 3 cut(s) 256, 1195, 1537
Ppu21I YACGTR 1 cut(s) 1073
PscI ACATGT 1 cut(s) 2026
Psp124BI GAGCTC 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 2085
PspGI CCWGG 1 cut(s) 2085
PspN4I GGNNCC 6 cut(s) 60, 267, 388, 686, 769, 1886
PspOMI GGGCCC 1 cut(s) 767
PspPI GGNCC 7 cut(s) 266, 767, 768, 917, 1489, 1606, 2270
PspXI VCTCGAGB 1 cut(s) 158
PstI CTGCAG 2 cut(s) 1852, 2302
PsuI RGATCY 4 cut(s) 518, 684, 1399, 2098
PvuII CAGCTG 3 cut(s) 337, 464, 1853
RsaI GTAC 5 cut(s) 156, 341, 597, 1871, 2077
RsaNI GTAC 5 cut(s) 155, 340, 596, 1870, 2076
RseI CAYNNNNRTG 3 cut(s) 288, 1071, 1455
SacI GAGCTC 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 7 cut(s) 237, 288, 980, 1142, 1476, 1616, 2332
SatI GCNGC 5 cut(s) 462, 465, 1052, 1851, 2291
Sau96I GGNCC 7 cut(s) 266, 767, 768, 917, 1489, 1606, 2270
ScaI AGTACT 2 cut(s) 156, 2077
SchI GAGTC 3 cut(s) 257, 1196, 1538
ScrFI CCNGG 3 cut(s) 2087, 2273, 2274
SduI GDGCHC 5 cut(s) 63, 321, 771, 1261, 1332
SfaNI GCATC 3 cut(s) 160, 955, 1390
SfcI CTRYAG 3 cut(s) 1109, 1848, 2298
Sfr274I CTCGAG 1 cut(s) 158
SfuI TTCGAA 1 cut(s) 1306
SinI GGWCC 3 cut(s) 266, 1489, 1606
SlaI CTCGAG 1 cut(s) 158
SmaI CCCGGG 1 cut(s) 2274
SmiMI CAYNNNNRTG 3 cut(s) 288, 1071, 1455
SmlI CTYRAG 3 cut(s) 158, 356, 1436
SmoI CTYRAG 3 cut(s) 158, 356, 1436
SpeI ACTAGT 1 cut(s) 2327
SphI GCATGC 2 cut(s) 1371, 2314
SsiI CCGC 8 cut(s) 43, 72, 100, 131, 232, 1054, 1559, 1633
SspMI CTAG 3 cut(s) 906, 1341, 2328
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 3 cut(s) 2085, 2271, 2272
StyI CCWWGG 3 cut(s) 859, 1340, 1908
TaaI ACNGT 6 cut(s) 409, 992, 1584, 1869, 1877, 2155
TaiI ACGT 3 cut(s) 195, 1075, 1785
TaqI TCGA 6 cut(s) 159, 189, 474, 1185, 1306, 1980
TatI WGTACW 3 cut(s) 154, 339, 2075
TfiI GAWTC 5 cut(s) 140, 1087, 1800, 1990, 2126
Tru1I TTAA 7 cut(s) 237, 288, 980, 1142, 1476, 1616, 2332
Tru9I TTAA 7 cut(s) 237, 288, 980, 1142, 1476, 1616, 2332
TscAI CASTG 8 cut(s) 88, 562, 1589, 1667, 1776, 1882, 2167, 2200
TseFI GTSAC 2 cut(s) 1019, 1919
TseI GCWGC 5 cut(s) 461, 464, 1051, 1850, 2290
Tsp45I GTSAC 2 cut(s) 1019, 1919
TspGWI ACGGA 1 cut(s) 272
TspMI CCCGGG 1 cut(s) 2272
TspRI CASTG 8 cut(s) 88, 562, 1589, 1667, 1776, 1882, 2167, 2200
VpaK11BI GGWCC 3 cut(s) 266, 1489, 1606
XagI CCTNNNNNAGG 1 cut(s) 2298
XapI RAATTY 3 cut(s) 819, 1181, 1592
XceI RCATGY 6 cut(s) 177, 904, 1336, 1371, 2030, 2314
XhoI CTCGAG 1 cut(s) 158
XmaI CCCGGG 1 cut(s) 2272
XmaJI CCTAGG 1 cut(s) 1340
XmiI GTMKAC 3 cut(s) 855, 1077, 1864
XspI CTAG 3 cut(s) 906, 1341, 2328
ZraI GACGTC 1 cut(s) 193
ZrmI AGTACT 2 cut(s) 156, 2077
Zsp2I ATGCAT 3 cut(s) 175, 1369, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.